Updated on 2025/07/03

写真a

 
SUZUKI Nobuhiro
 
Organization
Scheduled update Professor
Position
Professor
Profile

Dr. Nobuhiro SUZUKI received his M. S. (1985) in phytopathology and Ph. D (1989) in virology from Tohoku University in Sendai, Japan. Dr. SUZUKI currently serves as a full professor of the Institute of Plant Stress and Resources, formerly Institute of Plant Sciences and Bioresouces at Okayama University and as an Editor/Board member of Virus Research, Frontiers in Virology, Journal of General Plant Pathology, Virology Journal, Biology, Journal of Virology, and Virology. He has also been Guest Editors to PLoS Pathogens, PNAS, and mBio.

Suzuki Laboratory focuses on characterization of diverse viruses infecting phytopathogenic fungi and exploration of their interplays. Recent achievements include the discovery of a neo-virus lifestyle exhibited by a (+)ssRNA virus and an unrelated dsRNA virus in a plant pathogenic fungus. Prior to coming to Kurashiki, Okayama Prefecture, he was a visiting fellow of the Center for Agricultural Biotechnology at the University of Maryland Biotechnology Institute for four years (1997-2001) to study molecular biology of hypoviruses in the laboratory of Professor Donald L. Nuss. Before visiting UMBI, he served as an assistant professor and a lecturer of the Biotechnology Institute at the Akita Prefectural College of Agriculture for 11 years (1988-1998) where he conducted a project on molecular characterization of rice dwarf phytoreovirus, a member of the family Reoviridae. He received awards from the Japanese Phytopathological Society of Japan and Japanese Society for Virology for his outstanding achievements in plant virology.

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Degree

  • 博士(農学) ( 東北大学 )

Research Interests

  • plant virus

  • RNA silencing

  • fungal virus

  • Virology

  • ウイルス学

  • biocontrol

  • antiviral defense

Research Areas

  • Environmental Science/Agriculture Science / Plant protection science

Education

  • Tohoku University   農学研究科   農学

    - 1989

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    Country: Japan

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  • Tohoku University    

    - 1989

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  • Tohoku University   農学部   農学科

    - 1983

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    Country: Japan

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  • Tohoku University    

    - 1983

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Research History

  • Swiss Federal Research Institute WSL   Forest Health & Biotic Interactions   WSL Fellow

    2019.6 - 2019.11

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    Country:Switzerland

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  • Institute of Plant Science and Resources   Group of Plant/Microbe Interactions   Professor

    2007.4

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  • Research Institute for Bioresources   Group of Plant/Microbe Interactions   Associate Professor

    2001.4 - 2007.3

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  • University of Maryland Biotechnology Institute   Center for Agricultural Biotechnology   Visiting Associate Professor

    1998.4 - 2001.3

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  • University of Maryland Biotechnology Institute   Center for Agricultural Biotechnology   Visiting Scientist

    1996.4 - 1997.3

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  • Akita Prefectural College of Agriculture   Biotechnology Institute,   Lecturer

    1994.4 - 1998.3

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  • National Agriculture Research Organization   Visiting Scientist

    1994.4 - 1996.3

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  • Akita Prefectural College of Agriculture   Biotechnology Institute   Assistant Professor

    1988.5 - 1990.3

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Professional Memberships

Committee Memberships

  • mBio   Editor  

    2025.4   

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  • International Committee on Taxonomy of Viruses (ICTV)   ICTV Executive Committee Member  

    2020.10   

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  • Biology -Basel-   Editor  

    2019.1   

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  • PLoS Pathogens   Guest Editor  

    2017.1   

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  • Journal of Virology   Editorial Board Member  

    2016.1   

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  • Virology   Editorial Board Member  

    2015.1   

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  • Proceedings of the National Academy of Science, U. S. A.   Guest Editor  

    2014.1 - 2015.6   

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  • Frontiers in Virology   Editor  

    2011.1   

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  • Virus Research   Editor  

    2010.12   

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  • Viruses   Editorial Board Member  

    2022.9   

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    Committee type:Other

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  • The Japanese Society for Virology   Board of Directors  

    2022.1   

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  • Editor   Archives of Virology  

    2022.1   

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  • ICTV Study Group of the family Totiviridae   Study Group Chair  

    2020.1   

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  • Journal of General Plant Pathology   sub-Editor-in-Chief  

    2020.1 - 2021.12   

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  • The Japanese Society for Virology   Scientific Program Committee Member  

    2019.4 - 2020.3   

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  • Virology Journal   Editor  

    2019.1   

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  • The Phytopathological Society of Japan   Plant Virus Taxonomy Committee Chair  

    2018.4   

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  • The Phytopathological Society of Japan   Board of Directors  

    2018.1   

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  • The Japanese Society for Virology   Screening Committee Member  

    2018.1 - 2019.12   

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  • Frontiers in Cellular and Infection Microbiology   Guest Editor  

    2018.1 - 2019.12   

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  • mBio   Guest Editor  

    2017.1 - 2017.12   

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  • Journal of General Plant Pathology   Editor  

    2016.1 - 2020.12   

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  • The Japanese Society for Virology   Board of Directors  

    2016.1 - 2019.12   

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  • The Japanese Society for Virology   Board Member  

    2015.1   

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  • ICTV Study Group of the family Partitivirus   Study Group Member  

    2015.1 - 2020.12   

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  • ICTV Study Group of the family Hypoviridae   Study Group Chair  

    2015.1 - 2020.12   

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  • The Japanese Society for Virology   Scientific Program Committee Member  

    2013.4 - 2017.3   

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  • ICTV Study Group of the family Reoviridae   Study Group Member  

    2012.1   

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  • ICTV Study Group of the family Chrysoviridae and Quadriviridae   Study Group Member  

    2012   

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  • ICTV Study Group of the family Partitiviridae   Study Group Chair  

    2011.1 - 2014.12   

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  • The Japanese Society for Virology   Editor to UIRUS  

    2010.1 - 2013.12   

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  • 日本ウイルス学会   広報委員会委員  

    2009 - 2012   

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    Committee type:Academic society

    日本ウイルス学会

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  • Virus Research   Editorial Board Member  

    2007.12 - 2010.11   

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  • The Phytopathological Society of Japan   PSJ Plant Virus Disease Committee Member  

    2004.4   

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  • The Phytopathological Society of Japan   Plant Virus taxonomy Committee Member  

    2004.4 - 2018.3   

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  • ICTV Study Group of the family Hypoviridae   Study Group Member  

    2000.1 - 2014.12   

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Papers

  • RNA editing of genomic neighbors controls antiviral response in fungi

    Shinji Honda, Ayumi Yokoyama, Nobuhiro Suzuki

    Cell Host & Microbe   2025.3

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.chom.2025.02.016

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  • Argonaute-independent, Dicer-dependent antiviral defense against RNA viruses. Reviewed International journal

    Yukiyo Sato, Hideki Kondo, Nobuhiro Suzuki

    Proceedings of the National Academy of Sciences of the United States of America   121 ( 25 )   e2322765121   2024.6

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Antiviral RNA interference (RNAi) is conserved from yeasts to mammals. Dicer recognizes and cleaves virus-derived double-stranded RNA (dsRNA) and/or structured single-stranded RNA (ssRNA) into small-interfering RNAs, which guide effector Argonaute to homologous viral RNAs for digestion and inhibit virus replication. Thus, Argonaute is believed to be essential for antiviral RNAi. Here, we show Argonaute-independent, Dicer-dependent antiviral defense against dsRNA viruses using Cryphonectria parasitica (chestnut blight fungus), which is a model filamentous ascomycetous fungus and hosts a variety of viruses. The fungus has two dicer-like genes (dcl1 and dcl2) and four argonaute-like genes (agl1 to agl4). We prepared a suite of single to quadruple agl knockout mutants with or without dcl disruption. We tested these mutants for antiviral activities against diverse dsRNA viruses and ssRNA viruses. Although both DCL2 and AGL2 worked as antiviral players against some RNA viruses, DCL2 without argonaute was sufficient to block the replication of other RNA viruses. Overall, these results indicate the existence of a Dicer-alone defense and different degrees of susceptibility to it among RNA viruses. We discuss what determines the great difference in susceptibility to the Dicer-only defense.

    DOI: 10.1073/pnas.2322765121

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  • Replication of single viruses across the kingdoms, Fungi, Plantae, and Animalia. Reviewed International journal

    Paul Telengech, Kiwamu Hyodo, Hiroaki Ichikawa, Ryusei Kuwata, Hideki Kondo, Nobuhiro Suzuki

    Proceedings of the National Academy of Sciences of the United States of America   121 ( 25 )   e2318150121   2024.6

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    It is extremely rare that a single virus crosses host barriers across multiple kingdoms. Based on phylogenetic and paleovirological analyses, it has previously been hypothesized that single members of the family Partitiviridae could cross multiple kingdoms. Partitiviridae accommodates members characterized by their simple bisegmented double-stranded RNA genome; asymptomatic infections of host organisms; the absence of an extracellular route for entry in nature; and collectively broad host range. Herein, we show the replicability of single fungal partitiviruses in three kingdoms of host organisms: Fungi, Plantae, and Animalia. Betapartitiviruses of the phytopathogenic fungusRosellinia necatrix could replicate in protoplasts of the carrot (Daucus carota), Nicotiana benthamiana and Nicotiana tabacum, in some cases reaching a level detectable by agarose gel electrophoresis. Moreover, betapartitiviruses showed more robust replication than the tested alphapartitiviruses. One of the fungal betapartitiviruses, RnPV18, could persistently and stably infect carrot plants regenerated from virion-transfected protoplasts. Both alpha- and betapartitiviruses, although with different host preference, could replicate in two insect cell lines derived from the fall armyworm Spodoptera frugiperda and the fruit fly Drosophila melanogaster. Our results indicate the replicability of single partitiviruses in members of three kingdoms and provide insights into virus adaptation, host jumping, and evolution.

    DOI: 10.1073/pnas.2318150121

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  • Three-Layered Complex Interactions among Capsidless (+)ssRNA Yadokariviruses, dsRNA Viruses, and a Fungus Reviewed

    Yukiyo Sato, Sakae Hisano, Carlos José López-Herrera, Hideki Kondo, Nobuhiro Suzuki

    mBio   2022.8

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    A capsidless (+)ssRNA virus YkV1 (family Yadokariviridae ) highjacks the capsid of an unrelated dsRNA virus YnV1 (proposed family “ Yadonushiviridae ”) in a phytopathogenic ascomycete, while YkV1 trans -enhances YnV1 replication. Herein, we identified the dsRNA virus partners of three yadokariviruses (YkV3, YkV4a, and YkV4b) with genome organization different from YkV1 as being different from YnV1 at the suborder level.

    DOI: 10.1128/mbio.01685-22

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  • Mycovirus Diversity and Evolution Revealed/Inferred from Recent Studies. International journal

    Hideki Kondo, Leticia Botella, Nobuhiro Suzuki

    Annual review of phytopathology   2022.5

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    High-throughput virome analyses with various fungi, from cultured or uncultured sources, have led to the discovery of diverse viruses with unique genome structures and even neo-lifestyles. Examples in the former category include splipalmiviruses and ambiviruses. Splipalmiviruses, related to yeast narnaviruses, have multiple positive-sense (+) single-stranded (ss) RNA genomic segments that separately encode the RNA-dependent RNA polymerase motifs, the hallmark of RNA viruses (members of the kingdom Orthornavirae). Ambiviruses appear to have an undivided ssRNA genome of 3∼5 kb with two large open reading frames (ORFs) separated by intergenic regions. Another narna-like virus group has two fully overlapping ORFs on both strands of a genomic segment that span more than 90% of the genome size. New virus lifestyles exhibited by mycoviruses include the yado-kari/yado-nushi nature characterized by the partnership between the (+)ssRNA yadokarivirus and an unrelated dsRNA virus (donor of the capsid for the former) and the hadaka nature of capsidless 10-11 segmented (+)ssRNA accessible by RNase in infected mycelial homogenates. Furthermore, dsRNA polymycoviruses with phylogenetic affinity to (+)ssRNA animal caliciviruses have been shown to be infectious as dsRNA-protein complexes or deproteinized naked dsRNA. Many previous phylogenetic gaps have been filled by recently discovered fungal and other viruses, which have provided interesting evolutionary insights. Phylogenetic analyses and the discovery of natural and experimental cross-kingdom infections suggest that horizontal virus transfer may have occurred and continue to occur between fungi and other kingdoms. Expected final online publication date for the Annual Review of Phytopathology, Volume 60 is August 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

    DOI: 10.1146/annurev-phyto-021621-122122

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  • Proof of concept of the Yadokari nature: A capsidless replicase-encoding but replication-dependent positive-sense single-stranded RNA virus hosted by an unrelated double-stranded RNA virus Reviewed

    Subha Das, Md Mahfuz Alam, Rui Zhang, Sakae Hisano, Nobuhiro Suzuki

    Journal of Virology   95 ( 17 )   e00467-21   2021.9

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/JVI.00467-21

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  • Establishment of Neurospora crassa as a model organism for fungal virology Reviewed

    Shinji Honda, Ana Eusebio-Cope, Shuhei Miyashita, Ayumi Yokoyama, Annisa Aulia, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki

    Nature Communications   11 ( 1 )   5627   2020.12

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-020-19355-y

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  • Hadaka virus 1: A capsidless eleven-segmented positive-sense single-stranded RNA virus from a phytopathogenic fungus, Fusarium oxysporum Reviewed International journal

    Yukiyo Sato, Wajeeha Shamsi, Atif Jamal, Muhammad Faraz Bhatti, Hideki Kondo, Nobuhiro Suzuki, Reed B. Wickner

    mBio   11 ( 3 )   e0045-20   2020.5

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    DOI: 10.1128/mBio.00450-20

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  • Dicer functions transcriptionally and posttranscriptionally in a multilayer antiviral defense. Reviewed

    Andika IB, Kondo H, Suzuki N

    Proceedings of the National Academy of Sciences of the United States of America   116 ( 6 )   2274 - 2281   2019.2

  • SAGA complex mediates the transcriptional up-regulation of antiviral RNA silencing Reviewed

    Ida Bagus Andika, Atif Jamal, Hideki Kondo, Nobuhiro Suzuki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 17 )   E3499 - E3506   2017.4

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    DOI: 10.1073/pnas.1701196114

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  • Harnessing host ROS-generating machinery for the robust genome replication of a plant RNA virus Reviewed

    Kiwamu Hyodo, Kenji Hashimoto, Kazuyuki Kuchitsu, Nobuhiro Suzuki, Tetsuro Okuno

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 7 )   E1282 - E1290   2017.2

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    DOI: 10.1073/pnas.1610212114

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  • A capsidless ssRNA virus hosted by an unrelated dsRNA virus Reviewed

    Rui Zhang, Sakae Hisano, Akio Tani, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    Nature Microbiology   1 ( 1 )   2016.1

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    DOI: 10.1038/nmicrobiol.2015.1

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  • Highly activated RNA silencing via strong induction of dicer by one virus can interfere with the replication of an unrelated virus Reviewed

    Sotaro Chiba, Nobuhiro Suzuki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   112 ( 35 )   E4911 - E4918   2015.9

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    DOI: 10.1073/pnas.1509151112

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  • Mycoreovirus genome rearrangements associated with RNA silencing deficiency Reviewed

    Ana Eusebio-Cope, Nobuhiro Suzuki

    NUCLEIC ACIDS RESEARCH   43 ( 7 )   3802 - 3813   2015.4

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    DOI: 10.1093/nar/gkv239

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  • A capsidless (+)RNA yadokarivirus hosted by a dsRNA virus is infectious as particles, cDNA, and dsRNA. International journal

    Muhammad Fadli, Sakae Hisano, Guy Novoa, José R Castón, Hideki Kondo, Nobuhiro Suzuki

    Journal of virology   99 ( 3 )   e0216624   2025.3

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    Capsidless yadokariviruses (members of the order Yadokarivirales) with (+)RNA genomes divert the capsid of their partner icosahedral double-stranded RNA (dsRNA) viruses in different families of the order Ghabrivirales into the replication site. A yadokarivirus, AfSV2, has been reported from a German strain of the ascomycete fungus Aspergillus foetidus coinfected by two dsRNA viruses, a victorivirus (AfSV1, family Pseudototiviridae) and an alternavirus (AfFV, family Alternaviridae). Here, we identified AfSV1 as the partner of AfSV2 in a Japanese A. foetidus strain after showing the infectiousness of AfSV2 in three forms: virus particles (heterocapsid), transforming full-length complementary DNA (cDNA), and purified replicated form (RF) dsRNA that is believed to be inactive as a translational template. Virion transfection of virus-free A. foetidus protoplasts resulted in the generation of two strains infected either by AfSV1 alone or by both AfSV1 and AfSV2. Transformants with AfSV2 full-length cDNA launched AfSV2 infection only in the presence of AfSV1, but not those with AfSV2 RNA-directed RNA polymerase mutant cDNA. The purified fractions containing AfSV2 RF dsRNA also launched infection when transfected into protoplasts infected by AfSV1. Treatment with dsRNA-specific RNase III, but not with proteinase K, S1 nuclease, or DNase I, abolished the infectivity of AfSV2 RF dsRNA. Furthermore, we confirmed the infectiousness of gel-purified AfSV2 RF dsRNA in the presence of AfSV1. Taken together, our results show the unique infectious entity of AfSV2 and the expansion of yadokarivirus partners in the family Pseudototiviridae and provide interesting evolutionary insights.IMPORTANCEThe viral phylum Pisuviricota accommodates members with both double-stranded RNA (dsRNA) and (+)RNA genomes. Some members of the second group display peculiar virus lifestyles. These include (+)RNA yadkariviruses, which are capsidless and highjack the capsid of their partner dsRNA viruses in the order Ghabrivirales of a different phylum Duplornaviricota. We identified the partner dsRNA virus (AfSV1, a victorivirus) of a yadokarivirus (AfSV2) from the ascomycete Aspergillus foetidus. AfSV2 is infectious in the presence of AfSV1 in three forms: purified particles, transforming full-length complementary DNA, and, surprisingly, the purified replicative form dsRNA. These combined results expand yadokarivirus partner viruses to the family Pseudototiviridae and provide evidence for AfSV2 as a unique infectious entity as well as interesting evolutionary insights.

    DOI: 10.1128/jvi.02166-24

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  • Evidence for the replication of a plant rhabdovirus in its arthropod mite vector. International journal

    Hideki Kondo, Miki Fujita, Paul Telengech, Kazuyuki Maruyam, Kiwamu Hyodo, Aline Daniele Tassi, Ronald Ochoa, Ida Bagus Andika, Nobuhiro Suzuki

    Virus research   351   199522 - 199522   2025.1

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    Transmission of plant viruses that replicate in the insect vector is known as persistent-propagative manner. However, it remains unclear whether such virus-vector relationships also occur between plant viruses and other biological vectors such as arthropod mites. In this study, we investigated the possible replication of orchid fleck virus (OFV), a segmented plant rhabdovirus, within its mite vector (Brevipalpus californicus s.l.) using quantitative RT-qPCR, western blotting and next-generation sequencing. Time-course RT-qPCR and western blot analyses showed an increasing OFV accumulation pattern in mites after virus acquisition. Since OFV genome expression requires the transcription of polyadenylated mRNAs, polyadenylated RNA fractions extracted from the viruliferous mite samples and OFV-infected plant leaves were used for RNA-seq analysis. In the mite and plant datasets, a large number of sequence reads were aligned to genomic regions of OFV RNA1 and RNA2 corresponding to transcribed viral gene mRNAs. This includes the short polyadenylated transcripts originating from the leader and trailer regions at the ends of the viral genome, which are believed to play a crucial role in viral transcription/replication. In contrast, a low number of reads were mapped to the non-transcribed regions (gene junctions). These results strongly suggested that OFV gene expression occurs both in mites and plants. Additionally, deep sequencing revealed the accumulation of OFV-derived small RNAs in mites, although their size profiles differ from those found in plants. Taken together, our results indicated that OFV replicates within a mite vector and is targeted by the RNA-silencing mechanism.

    DOI: 10.1016/j.virusres.2024.199522

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  • Changes to virus taxonomy and the ICTV Statutes ratified by the International Committee on Taxonomy of Viruses (2024) Reviewed

    Peter Simmonds, Evelien M. Adriaenssens, Elliot J. Lefkowitz, Hanna M. Oksanen, Stuart G. Siddell, Francisco Murilo Zerbini, Poliane Alfenas-Zerbini, Frank O. Aylward, Donald M. Dempsey, Bas E. Dutilh, Juliana Freitas-Astúa, María Laura García, R. Curtis Hendrickson, Holly R. Hughes, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Arcady R. Mushegian, Judit Penzes, Alejandro Reyes Muñoz, David L. Robertson, Simon Roux, Luisa Rubino, Sead Sabanadzovic, Donald B. Smith, Nobuhiro Suzuki, Dann Turner, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani

    Archives of Virology   169 ( 11 )   2024.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    This article reports changes to virus taxonomy and taxon nomenclature that were approved and ratified by the International Committee on Taxonomy of Viruses (ICTV) in April 2024. The entire ICTV membership was invited to vote on 203 taxonomic proposals that had been approved by the ICTV Executive Committee (EC) in July 2023 at the 55th EC meeting in Jena, Germany, or in the second EC vote in November 2023. All proposals were ratified by online vote. Taxonomic additions include one new phylum (Ambiviricota), one new class, nine new orders, three new suborders, 51 new families, 18 new subfamilies, 820 new genera, and 3547 new species (excluding taxa that have been abolished). Proposals to complete the process of species name replacement to the binomial (genus + species epithet) format were ratified. Currently, a total of 14,690 virus species have been established.

    DOI: 10.1007/s00705-024-06143-y

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    Other Link: https://link.springer.com/article/10.1007/s00705-024-06143-y/fulltext.html

  • Virus Research: 40 years and still going strong Invited Reviewed

    Ben Berkhout, Esteban Domingo, Nobuhiro Suzuki

    Virus Research   350   199493 - 199493   2024.11

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.virusres.2024.199493

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  • Ancient environmental microbiomes and the cryosphere Reviewed

    Alexander D. Williams, Vivian W. Leung, Julian W. Tang, Nishimura Hidekazu, Nobuhiro Suzuki, Andrew C. Clarke, David A. Pearce, Tommy Tsan-Yuk Lam

    Trends in Microbiology   2024.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.tim.2024.09.010

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  • New lineages of RNA viruses from clinical isolates of Rhizopus microsporus revealed by fragmented and primer-ligated dsRNA sequencing (FLDS) analysis. Reviewed International journal

    Wasiatus Sa'diyah, Yan-Jie Zhao, Yuto Chiba, Hideki Kondo, Nobuhiro Suzuki, Sayaka Ban, Takashi Yaguchi, Syun-Ichi Urayama, Daisuke Hagiwara

    mSphere   9   e0034524   2024.7

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    Rhizopus microsporus is a species in the order Mucorales that is known to cause mucormycosis, but it is poorly understood as a host of viruses. Here, we examined 25 clinical strains of R. microsporus for viral infection with a conventional double-stranded RNA (dsRNA) assay using agarose gel electrophoresis (AGE) and the recently established fragmented and primer-ligated dsRNA sequencing (FLDS) protocol. By AGE, five virus-infected strains were detected. Then, full-length genomic sequences of 12 novel RNA viruses were revealed by FLDS, which were related to the families Mitoviridae, Narnaviridae, and Endornaviridae, ill-defined groups of single-stranded RNA (ssRNA) viruses with similarity to the established families Virgaviridae and Phasmaviridae, and the proposed family "Ambiguiviridae." All the characterized viruses, except a potential phasmavirid with a negative-sense RNA genome, had positive-sense RNA genomes. One virus belonged to a previously established species within the family Mitoviridae, whereas the other 11 viruses represented new species or even new genera. These results show that the fungal pathogen R. microsporus harbors diverse RNA viruses and extend our understanding of the diversity of RNA viruses in the fungal order Mucorales, division Mucoromycota. Identifying RNA viruses from clinical isolates of R. microsporus may expand the repertoire of natural therapeutic agents for mucormycosis in the future.IMPORTANCEThe diversity of mycoviruses in fungal hosts in the division Mucoromycota has been underestimated, mainly within the species Rhizopus microsporus. Only five positive-sense RNA genomes had previously been discovered in this species. Because current sequencing methods poorly complete the termini of genomes, we used fragmented and primer-ligated double-stranded RNA sequencing to acquire the full-length genomes. Eleven novel mycoviruses were detected in this study, including the first negative-sense RNA genome reported in R. microsporus. Our findings extend the understanding of the viral diversity in clinical strains of Mucoromycota, may provide insights into the pathogenesis and ecology of this fungus, and may offer therapeutic options.

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  • Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses. Reviewed International journal

    Michael Louie R Urzo, Timothy D Guinto, Ana Eusebio-Cope, Bernard O Budot, Mary Jeanie T Yanoria, Gilda B Jonson, Masao Arakawa, Hideki Kondo, Nobuhiro Suzuki

    Viruses   16 ( 7 )   2024.7

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    Rice sheath blight, caused by the soil-borne fungus Rhizoctonia solani (teleomorph: Thanatephorus cucumeris, Basidiomycota), is one of the most devastating phytopathogenic fungal diseases and causes yield loss. Here, we report on a very high prevalence (100%) of potential virus-associated double-stranded RNA (dsRNA) elements for a collection of 39 fungal strains of R. solani from the rice sheath blight samples from at least four major rice-growing areas in the Philippines and a reference isolate from the International Rice Research Institute, showing different colony phenotypes. Their dsRNA profiles suggested the presence of multiple viral infections among these Philippine R. solani populations. Using next-generation sequencing, the viral sequences of the three representative R. solani strains (Ilo-Rs-6, Tar-Rs-3, and Tar-Rs-5) from different rice-growing areas revealed the presence of at least 36 viruses or virus-like agents, with the Tar-Rs-3 strain harboring the largest number of viruses (at least 20 in total). These mycoviruses or their candidates are believed to have single-stranded RNA or dsRNA genomes and they belong to or are associated with the orders Martellivirales, Hepelivirales, Durnavirales, Cryppavirales, Ourlivirales, and Ghabrivirales based on their coding-complete RNA-dependent RNA polymerase sequences. The complete genome sequences of two novel RNA viruses belonging to the proposed family Phlegiviridae and family Mitoviridae were determined.

    DOI: 10.3390/v16071152

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  • Exploration of the yadokari/yadonushi nature of YkV3 and RnMBV3 in the original host and a model filamentous fungus. Reviewed International journal

    Yukiyo Sato, Sakae Hisano, Nobuhiro Suzuki

    Virus research   334   199155 - 199155   2023.6

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    The yadokari/yadonushi nature is a recently discovered virus lifestyle; "yadokari" refers to the ability of capsidless positive-sense (+) RNA viruses (yadokariviruses) to utilize the capsids of phylogenetically distant double-stranded RNA (dsRNA) viruses possibly as the replication site, while "yadonushi" refers to the ability of dsRNA viruses to provide capsids to yadokariviruses. This virus-virus interaction, however, has been only studied with limited pathosystems. Here, we established a new study model with a capsidless (+)RNA yadokarivirus YkV3 (family Yadokariviridae) and its capsid donor RnMBV3 (family Megabirnaviridae) in the original host fungus Rosellinia necatrix and a model filamentous fungal host Cryphonectria parasitica. YkV3 has a simple genome structure with one open reading frame of 4305 nucleotides encoding a single polyprotein with an RNA-dependent RNA polymerase and a 2A-like self-cleavage peptide domain. Reverse genetics of YkV3 in R. necatrix showed that YkV3 tolerates a nucleotide substitution in the extreme 5'-terminus. The insertion of two termination codons immediately downstream of the 2A-like cleavage site abolished YkV3 viability, suggesting the importance of the C-terminal portion of the polyprotein of unknown function. Transfection of RnMBV3 and YkV3 into an RNA silencing-deficient mutant Δdcl2 of C. parasitica showed the replication competency of both viruses. Comparison between the wild-type and Δdcl2 strains of C. parasitica in virus accumulation suggested that RnMBV3 and YkV3 are susceptible to RNA silencing in C. parasitica. Taken together, we have established a platform to further explore the yadokari/yadonushi nature using genetically manipulable host fungal and virus strains.

    DOI: 10.1016/j.virusres.2023.199155

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  • Continued mycovirus discovery expanding our understanding of virus lifestyles, symptom expression, and host defense. Reviewed International journal

    Yukiyo Sato, Nobuhiro Suzuki

    Current opinion in microbiology   75   102337 - 102337   2023.6

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    High-throughput sequencing technologies have greatly expanded the RNA virome in general and have led to an exponential increase in new fungal viruses, also known as mycoviruses. Mycoviruses are omnipresent in fungi and usually induce symptomless infections. Some mycoviruses infecting fungi pathogenic to plants, insects, and mammals are known to modify host virulence positively and negatively and attract particular interests. In addition, fungal viruses continue to provide intriguing research materials and themes that lead to discoveries of peculiar viruses as infectious entities and insights into virus evolution and diversity. In this review, we outline the diversity and neolifestyle of recently discovered fungal RNA viruses, and phenotypic alterations induced by them. Furthermore, we discuss recent advances in research regarding the fungal antiviral defense and viral counterdefense, which are closely associated with host phenotype alterations. We hope that this article will enhance understanding of the interesting and growing fungal virology field.

    DOI: 10.1016/j.mib.2023.102337

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  • Changes to virus taxonomy and the ICTV Statutes ratified by the International Committee on Taxonomy of Viruses (2023)

    Francisco Murilo Zerbini, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas‑Zerbini, Donald M. Dempsey, Bas E. Dutilh, María Laura García, R. Curtis Hendrickson, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne‑Mieke Vandamme, Arvind Varsani

    Archives of Virology   168 ( 7 )   175   2023.6

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    DOI: 10.1007/s00705-023-05797-4

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  • ICTV Virus Taxonomy Profile: Hypoviridae 2023 Reviewed

    Sotaro Chiba, Leonardo Velasco, María A. Ayllón, Nobuhiro Suzuki, Shin-Yi Lee-Marzano, Lying Sun, Sead Sabanadzovic, Massimo Turina

    Journal of General Virology   104 ( 5 )   001848   2023.5

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    Hypoviridae is a family of capsidless viruses with positive-sense RNA genomes of 7.3–18.3 kb that possess either a single large open reading frame (ORF) or two ORFs. The ORFs appear to be translated from genomic RNA by non-canonical mechanisms, i.e. internal ribosome entry site- and stop/restart translation. This family includes the genera Alphahypovirus, Betahypovirus, Gammahypovirus, Deltahypovirus, Epsilonhypovirus, Zetahypovirus, Thetahypovirus and Etahypovirus. Hypovirids have been detected in ascomycetous and basidiomycetous filamentous fungi and are considered to replicate in host, Golgi apparatus-derived, lipid vesicles that contain virus dsRNA as the replicative form. Some hypovirids induce hypovirulence to host fungi, while others do not. This is a summary of the ICTV report on the family Hypoviridae, which is available at www.ictv.global/report/hypoviridae.

    DOI: 10.1099/jgv.0.001848

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  • Sequence and phylogenetic analysis of a novel alphaendornavirus, the first virus described from the oomycete plant pathogen Phytophthora heveae Reviewed

    Milica Raco, Thomas Jung, Marilia Horta Jung, Nguyen Minh Chi, Leticia Botella, Nobuhiro Suzuki

    Archives of Virology   168 ( 6 )   2023.5

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    Abstract

    Here, we report the discovery and complete genome sequence of a novel virus, designated as “Phytophthora heveae alphaendornavirus 1” (PhAEV1), from a single isolate of the plant pathogenic oomycete Phytophthora heveae (kingdom Stramenipila) isolated from a tropical evergreen lowland rainforest in northern Vietnam. PhAEV1 was detected by both cellulose affinity chromatography of dsRNA and high-throughput sequencing of total RNA, and its presence and sequence were confirmed by RT-PCR and Sanger sequencing. The PhAEV1 genome, 12,820 nucleotides (nt) in length, was predicted to encode a single large polyprotein with the catalytic core domain of viral (superfamily 1) RNA helicase (HEL, amino acid [aa] positions 1,287-1,531), glycosyltransferase (GT, aa positions ca. 2,800-3,125), and RNA-directed RNA polymerase (RdRp, aa positions 3,875-4,112). PhAEV1 is the most similar to Phytophthora cactorum alphaendornavirus 3, sharing 39.4% and 39.1% nt and aa sequence identity, respectively. In addition to the first 5′-terminal AUG codon, three additional in-frame methionine codons were found in close proximity (nt 14-16, 96-98, and 176-178), suggesting potential additional translation initiation sites. Conserved RdRp motifs (A-E) similar to those detected in related endornaviruses were identified in PhAEV1, as well as in several previously described alphaendornaviruses from other Phytophthora species in which these motifs had not been identified previously. Phylogenetic analysis showed that PhAEV1 clusters with members of the genus Alphaendornavirus in the family Endornaviridae and is basal to two other alphaendornaviruses described from another oomycete, Phytophthora cactorum. PhAEV1 is the first virus reported in P. heveae.

    DOI: 10.1007/s00705-023-05786-7

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  • Virus taxonomy and the role of the International Committee on Taxonomy of Viruses (ICTV) Reviewed

    Stuart G. Siddell, Donald B. Smith, Evelien Adriaenssens, Poliane Alfenas-Zerbini, Bas E. Dutilh, Maria Laura Garcia, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Elliot J. Lefkowitz, Małgorzata Łobocka, Arcady R. Mushegian, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani, F. Murilo Zerbini

    Journal of General Virology   104 ( 5 )   2023.5

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    The taxonomy of viruses is developed and overseen by the International Committee on Taxonomy of Viruses (ICTV), which scrutinizes, approves and ratifies taxonomic proposals, and maintains a list of virus taxa with approved names (https://ictv.global). The ICTV has approximately 180 members who vote by simple majority. Taxon-specific Study Groups established by the ICTV have a combined membership of over 600 scientists from the wider virology community; they provide comprehensive expertise across the range of known viruses and are major contributors to the creation and evaluation of taxonomic proposals. Proposals can be submitted by anyone and will be considered by the ICTV irrespective of Study Group support. Thus, virus taxonomy is developed from within the virology community and realized by a democratic decision-making process. The ICTV upholds the distinction between a virus or replicating genetic element as a physical entity and the taxon category to which it is assigned. This is reflected by the nomenclature of the virus species taxon, which is now mandated by the ICTV to be in a binomial format (genus + species epithet) and is typographically distinct from the names of viruses. Classification of viruses below the rank of species (such as, genotypes or strains) is not within the remit of the ICTV. This article, authored by the ICTV Executive Committee, explains the principles of virus taxonomy and the organization, function, processes and resources of the ICTV, with the aim of encouraging greater understanding and interaction among the wider virology community.

    DOI: 10.1099/jgv.0.001840

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  • Structural features of T-DNA that induce transcriptional gene silencing during agroinfiltration Reviewed

    Emi Iida, Kazunori Kuriyama, Midori Tabara, Atsushi Takeda, Nobuhiro Suzuki, Hiromitsu Moriyama, Toshiyuki Fukuhara

    2023.4

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    <jats:title>Abstract</jats:title>
    <jats:p><jats:italic>Agrobacterium tumefaciens</jats:italic> (<jats:italic>Rhizobium radiobacter</jats:italic>) is used for the transient expression of foreign genes by the agroinfiltration method, but the introduction of foreign genes often induces transcriptional and/or post-transcriptional gene silencing (TGS and/or PTGS). In this study, we characterized the structural features of T-DNA that induce TGS during agroinfiltration. When <jats:italic>A. tumefaciens</jats:italic>cells harboring an empty T-DNA plasmid containing the cauliflower mosaic virus (CaMV) 35S promoter were infiltrated into the leaves<jats:italic> </jats:italic>of <jats:italic>Nicotiana benthamiana</jats:italic> line 16c with a GFP gene over-expressed under the control of the same promoter, no small interfering RNAs (siRNAs) were derived from the GFP sequence. However, siRNAs derived from the CaMV 35S promoter were detected, indicating that TGS against the GFP gene was induced. When the GFP gene was inserted into the T-DNA plasmid, PTGS against the GFP gene was induced whereas TGS against the CaMV 35S promoter was suppressed. In other words, depending on the combination of promoter and coding sequences on T-DNA and the host nuclear genome, either or both TGS and/or PTGS could be induced by agroinfiltration. We also showed the importance of terminator sequences in T-DNA for gene silencing and the possible involvement of three siRNA-producing Dicers in the induction of TGS. These results are valuable for controlling gene expression by agroinfiltration.</jats:p>

    DOI: 10.21203/rs.3.rs-2796710/v1

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  • Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan Reviewed

    Hideki Kondo, Hitomi Sugahara, Miki Fujita, Kiwamu Hyodo, Ida Bagus Andika, Hiroshi Hisano, Nobuhiro Suzuki

    Pathogens   12 ( 3 )   358 - 358   2023.2

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    Many aphid-borne viruses are important pathogens that affect wheat crops worldwide. An aphid-transmitted closterovirus named wheat yellow leaf virus (WYLV) was found to have infected wheat plants in Japan in the 1970s; however, since then, its viral genome sequence and occurrence in the field have not been investigated. We observed yellowing leaves in the 2018/2019 winter wheat-growing season in an experimental field in Japan where WYLV was detected five decades ago. A virome analysis of those yellow leaf samples lead to the discovery of a closterovirus together with a luteovirus (barley yellow dwarf virus PAV variant IIIa). The complete genomic sequence of this closterovirus, named wheat closterovirus 1 isolate WL19a (WhCV1-WL19a), consisted of 15,452 nucleotides harboring nine open reading frames. Additionally, we identified another WhCV1 isolate, WL20, in a wheat sample from the winter wheat-growing season of 2019/2020. A transmission test indicated that WhCV1-WL20 was able to form typical filamentous particles and transmissible by oat bird-cherry aphid (Rhopalosiphum pad). Sequence and phylogenetic analyses showed that WhCV1 was distantly related to members of the genus Closterovirus (family Closteroviridae), suggesting that the virus represents a novel species in the genus. Furthermore, the characterization of WhCV1-WL19a-derived small RNAs using high-throughput sequencing revealed highly abundant 22-nt-class small RNAs potentially derived from the 3′-terminal end of the WhCV1 negative-strand genomic RNA, indicating that this terminal end of the WhCV1 genome is likely particularly targeted for the synthesis of viral small RNAs in wheat plants. Our results provide further knowledge on closterovirus diversity and pathogenicity and suggest that the impact of WhCV1 on wheat production warrants further investigations.

    DOI: 10.3390/pathogens12030358

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  • Four principles to establish a universal virus taxonomy Reviewed

    Peter Simmonds, Evelien M. Adriaenssens, F. Murilo Zerbini, Nicola G. A. Abrescia, Pakorn Aiewsakun, Poliane Alfenas-Zerbini, Yiming Bao, Jakub Barylski, Christian Drosten, Siobain Duffy, W. Paul Duprex, Bas E. Dutilh, Santiago F. Elena, Maria Laura García, Sandra Junglen, Aris Katzourakis, Eugene V. Koonin, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Elliot J. Lefkowitz, Małgorzata Łobocka, Cédric Lood, Jennifer Mahony, Jan P. Meier-Kolthoff, Arcady R. Mushegian, Hanna M. Oksanen, Minna M. Poranen, Alejandro Reyes-Muñoz, David L. Robertson, Simon Roux, Luisa Rubino, Sead Sabanadzovic, Stuart Siddell, Tim Skern, Donald B. Smith, Matthew B. Sullivan, Nobuhiro Suzuki, Dann Turner, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani, Nikos Vasilakis

    PLOS Biology   21 ( 2 )   e3001922 - e3001922   2023.2

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    A universal taxonomy of viruses is essential for a comprehensive view of the virus world and for communicating the complicated evolutionary relationships among viruses. However, there are major differences in the conceptualisation and approaches to virus classification and nomenclature among virologists, clinicians, agronomists, and other interested parties. Here, we provide recommendations to guide the construction of a coherent and comprehensive virus taxonomy, based on expert scientific consensus. Firstly, assignments of viruses should be congruent with the best attainable reconstruction of their evolutionary histories, i.e., taxa should be monophyletic. This fundamental principle for classification of viruses is currently included in the International Committee on Taxonomy of Viruses (ICTV) code only for the rank of species. Secondly, phenotypic and ecological properties of viruses may inform, but not override, evolutionary relatedness in the placement of ranks. Thirdly, alternative classifications that consider phenotypic attributes, such as being vector-borne (e.g., “arboviruses”), infecting a certain type of host (e.g., “mycoviruses,” “bacteriophages”) or displaying specific pathogenicity (e.g., “human immunodeficiency viruses”), may serve important clinical and regulatory purposes but often create polyphyletic categories that do not reflect evolutionary relationships. Nevertheless, such classifications ought to be maintained if they serve the needs of specific communities or play a practical clinical or regulatory role. However, they should not be considered or called taxonomies. Finally, while an evolution-based framework enables viruses discovered by metagenomics to be incorporated into the ICTV taxonomy, there are essential requirements for quality control of the sequence data used for these assignments. Combined, these four principles will enable future development and expansion of virus taxonomy as the true evolutionary diversity of viruses becomes apparent.

    DOI: 10.1371/journal.pbio.3001922

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  • ICTV Virus Taxonomy Profile: Hadakaviridae. Invited Reviewed

    Sato, Y, Turina, M, Chiba, S, Okada, R. A, Bhatti, M. F, Kotta-Loizou, I, Coutts, R. H. A, Kondo, H, Sabanadzovic, S, Suzuki, N, ICTV Repor, Consortium

    Journal of General Virology   104   001820   2023.2

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  • Capsid structure of a fungal dsRNA megabirnavirus reveals its previously unidentified surface architecture Reviewed

    Wang, H, Salaipeth, L, Miyazaki, N, Suzuki, N, Okamoto, K

    PLoS Pathogens   19   e1011162 - e1011162   2023.2

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    DOI: 10.1371/journal.ppat.1011162

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  • ICTV Virus Taxonomy Profile: Yadokariviridae 2023 Reviewed

    Yukiyo Sato, Subha Das, Leonardo Velasco, Massimo Turina, Hideki Osaki, Ioly Kotta-Loizou, Robert H. A. Coutts, Hideki Kondo, Sead Sabanadzovic, Nobuhiro Suzuki

    Journal of General Virology   104 ( 1 )   001826   2023.1

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    The family Yadokariviridae, with the genera Alphayadokarivirus and Betayadokarivirus, includes capsidless non-segmented positive-sense (+) RNA viruses that hijack capsids from phylogenetically distant double-stranded RNA viruses. Yadokarivirids likely replicate inside the hijacked heterocapsids using their own RNA-directed RNA polymerase, mimicking dsRNA viruses despite their phylogenetic placement in a (+) RNA virus lineage. Yadokarivirids can have negative or positive impacts on their host fungi, through interactions with the capsid donor dsRNA viruses. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) report on the family Yadokariviridae, which is available at ictv.global/report/yadokariviridae.

    DOI: 10.1099/jgv.0.001826

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  • The simplest RNA replicons, viroids: A tribute to Ricardo Flores Invited

    Vicente Pallas, Francesco Di Serio, Nobuhiro Suzuki

    Virus Research   323   198996 - 198996   2023.1

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    DOI: 10.1016/j.virusres.2022.198996

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  • ICTV Virus Taxonomy Profile: Spinareoviridae 2022 Reviewed

    Jelle Matthijnssens, Houssam Attoui, Krisztián Bányai, Corina P. D. Brussaard, Pranav Danthi, Mariana del Vas, Terence S. Dermody, Roy Duncan, Qín Fāng, Reimar Johne, Peter P. C. Mertens, Fauziah Mohd Jaafar, John T. Patton, Takahide Sasaya, Nobuhiro Suzuki, Taiyun Wei

    Journal of General Virology   103 ( 11 )   2022.11

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    Spinareoviridae is a large family of icosahedral viruses that are usually regarded as non-enveloped with segmented (9–12 linear segments) dsRNA genomes of 23–29 kbp. Spinareovirids have a broad host range, infecting animals, fungi and plants. Some have important pathogenic potential for humans (e.g. Colorado tick fever virus), livestock (e.g. avian orthoreoviruses), fish (e.g. aquareoviruses) and plants (e.g. rice ragged stunt virus and rice black streaked dwarf virus). This is a summary of the ICTV Report on the family Spinareoviridae, which is available at ictv.global/report/spinareoviridae.

    DOI: 10.1099/jgv.0.001781

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  • Recent changes to virus taxonomy ratified by the International Committee on Taxonomy of Viruses (2022) Reviewed

    Peter J. Walker, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Donald M. Dempsey, Bas E. Dutilh, María Laura García, R. Curtis Hendrickson, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Hanna M. Oksanen, Richard J. Orton, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani, Francisco Murilo Zerbini

    Archives of Virology   167 ( 11 )   2429 - 2440   2022.11

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    DOI: 10.1007/s00705-022-05516-5

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  • Identification of novel totiviruses from the ascomycetous fungus Geotrichum candidum. International journal

    Haris Ahmed Khan, Hideki Kondo, Sabitree Shahi, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of virology   2022.10

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    Mycoviruses are widely distributed across the kingdom Fungi, including ascomycetous yeast strains of the class Saccharomycetes. Geotrichum candidum is an important fungal pathogen belonging to Saccharomycetes and has a diverse host range. Here, we report the characterization of four new classical totiviruses from two distinct Geotrichum candidum strains from Pakistan. The four identified viruses were tentatively named "Geotrichum candidum totivirus 1, 2, 3a, and 3b" (GcTV1-3b). The complete dsRNA genomes of the identified totiviruses are 4621, 4592, 4576, and 4576 bp in length, respectively. All totivirus genomes have two open reading frames, encoding a capsid protein (CP) and an RNA-dependent RNA polymerase (RdRP), respectively. The downstream RdRP domain is assumed to be expressed as a CP-RdRP fusion product via -1 frameshifting mediated by a heptameric slippery site. Sequence comparisons and phylogenetic analysis showed that each of the discovered viruses belongs to a new species of the genus Totivirus in the family Totiviridae, with GcTV1 and GcTV3 (a and b strains) clustering in one subgroup and GcTV2 in another subgroup.

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  • ICTV Virus Taxonomy Profile: Sedoreoviridae 2022 Reviewed

    Jelle Matthijnssens, Houssam Attoui, Krisztián Bányai, Corina P. D. Brussaard, Pranav Danthi, Mariana del Vas, Terence S. Dermody, Roy Duncan, Qín Fāng (方勤), Reimar Johne, Peter P. C. Mertens, Fauziah Mohd Jaafar, John T. Patton, Takahide Sasaya (笹谷孝英), Nobuhiro Suzuki (鈴木信弘), Taiyun Wei (魏太云)

    Journal of General Virology   103 ( 10 )   2022.10

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    Sedoreoviridae is a large family of icosahedral viruses that are usually regarded as non-enveloped with segmented (10–12 linear segments) dsRNA genomes of 18–26 kbp. Sedoreovirids have a broad host range, infecting mammals, birds, crustaceans, arthropods, algae and plants. Some of them have important pathogenic potential for humans (e.g. rotavirus A), livestock (e.g. bluetongue virus) and plants (e.g. rice dwarf virus). This is a summary of the ICTV Report on the family Sedoreoviridae, which is available at ictv.global/report/sedoreoviridae.

    DOI: 10.1099/jgv.0.001782

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  • A Transfectable Fusagravirus from a Japanese Strain of Cryphonectria carpinicola with Spherical Particles. Reviewed International journal

    Subha Das, Sakae Hisano, Ana Eusebio-Cope, Hideki Kondo, Nobuhiro Suzuki

    Viruses   14 ( 8 )   2022.8

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    A novel dsRNA virus (Cryphonectria carpinicola fusagravirus 1, CcFGV1), isolated from a Japanese strain (JS13) of Cryphonectria carpinicola, was thoroughly characterized. The biological comparison of a set of isogenic CcFGV1-infected and -free (JS13VF) strains indicated asymptomatic infection by CcFGV1. The sequence analysis showed that the virus has a two open reading frame (ORF) genome of 9.6 kbp with the RNA-directed RNA polymerase domain encoded by ORF2. The N-terminal sequencing and peptide mass fingerprinting showed an N-terminally processed or degraded product (150 kDa) of the 5'-proximal ORF1-encoded protein (1462 amino acids) to make up the CcFGV1 spherical particles of ~40 nm in diameter. Interestingly, a portion of CcFGV1 dsRNA co-fractionated with a host protein of 70 kDa. The purified CcFGV1 particles were used to transfect protoplasts of JS13VF as well as the standard strain of an experimental model filamentous fungal host Cryphonectria parasitica. CcFGV1 was confirmed to be associated with asymptomatic infection of both fungi. RNA silencing was shown to target the virus in C. parasitica, resulting in reduced CcFGV1 accumulation by comparing the CcFGV1 content between RNA silencing-competent and -deficient strains. These results indicate the transfectability of spherical particles of a fusagravirus associated with asymptomatic infection.

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  • Mycovirus Hunting Revealed the Presence of Diverse Viruses in a Single Isolate of the Phytopathogenic Fungus Diplodia seriata From Pakistan Reviewed

    Haris Ahmed Khan, Paul Telengech, Hideki Kondo, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Frontiers in Cellular and Infection Microbiology   12   2022.6

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    Diplodia seriata in the family Botryosphaeriaceae is a cosmopolitan phytopathogenic fungus and is responsible for causing cankers, fruit rot and leaf spots on economically important plants. In this study, we characterized the virome of a single Pakistani strain (L3) of D. seriata. Several viral-like contig sequences were obtained via a previously conducted next-generation sequencing analysis. Multiple infection of the L3 strain by eight RNA mycoviruses was confirmed through RT-PCR using total RNA samples extracted from this strain; the entire genomes were determined via Sanger sequencing of RT-PCR and RACE clones. A BLAST search and phylogenetic analyses indicated that these eight mycoviruses belong to seven different viral families. Four identified mycoviruses belong to double-stranded RNA viral families, including Polymycoviridae, Chrysoviridae, Totiviridae and Partitiviridae, and the remaining four identified mycoviruses belong to single-stranded RNA viral families, i.e., Botourmiaviridae, and two previously proposed families “Ambiguiviridae” and “Splipalmiviridae”. Of the eight, five mycoviruses appear to represent new virus species. A morphological comparison of L3 and partially cured strain L3ht1 suggested that one or more of the three viruses belonging to Polymycoviridae, “Splipalmiviridae” and “Ambiguiviridae” are involved in the irregular colony phenotype of L3. To our knowledge, this is the first report of diverse virome characterization from D. seriata.

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  • A novel deltapartitivirus from red clover. Reviewed International journal

    Paul Telengech, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki

    Archives of virology   167 ( 4 )   1201 - 1204   2022.4

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    The family Partitiviridae has five genera, among which is the genus Deltapartitivirus. We report here the complete genome sequence of a deltapartitivirus from red clover, termed "red clover cryptic virus 3" (RCCV3). RCCV3 has a bisegmented double-stranded (ds) RNA genome. dsRNA1 and dsRNA2 are 1580 and 1589 nucleotides (nt) in length and are predicted to encode an RNA-directed RNA polymerase (RdRP) and a capsid protein (CP), respectively. The RCCV3 RdRP shares the highest sequence identity with the RdRP of a previously reported deltapartitivirus, Medicago sativa deltapartitivirus 1 (MsDPV1) (76.5%), while the RCCV3 CP shows 50% sequence identity to the CP of MsDPV1. RdRP- and CP-based phylogenetic trees place RCCV3 into a clade of deltapartitiviruses. The sequence and phylogenetic analyses clearly indicate that RCCV3 represents a new species in the genus Deltapartitivirus. RCCV3 was detectable in all three tested cultivars of red clover.

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  • Differentiating between viruses and virus species by writing their names correctly Reviewed

    Francisco Murilo Zerbini, Stuart G. Siddell, Arcady R. Mushegian, Peter J. Walker, Elliot J. Lefkowitz, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Bas E. Dutilh, María Laura García, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani

    Archives of Virology   167 ( 4 )   1231 - 1234   2022.4

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    DOI: 10.1007/s00705-021-05323-4

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  • Plant viruses and viroids in Japan Invited Reviewed

    Shin-ichi Fuji, Tomofumi Mochizuki, Mitsuru Okuda, Shinya Tsuda, Satoshi Kagiwada, Ken-Taro Sekine, Masashi Ugaki, Keiko T. Natsuaki, Masamichi Isogai, Tetsuo Maoka, Minoru Takeshita, Nobuyuki Yoshikawa, Kazuyuki Mise, Takahide Sasaya, Hideki Kondo, Kenji Kubota, Yasuyuki Yamaji, Toru Iwanami, Kazusato Ohshima, Kappei Kobayashi, Tatsuji Hataya, Teruo Sano, Nobuhiro Suzuki

    Journal of General Plant Pathology   88 ( 2 )   105 - 127   2022.3

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    Abstract

    An increasing number of plant viruses and viroids have been reported from all over the world due largely to metavirogenomics approaches with technological innovation. Herein, the official changes of virus taxonomy, including the establishment of megataxonomy and amendments of the codes of virus classification and nomenclature, recently made by the International Committee on Taxonomy of Viruses were summarized. The continued efforts of the plant virology community of Japan to index all plant viruses and viroids occurring in Japan, which represent 407 viruses, including 303 virus species and 104 unclassified viruses, and 25 viroids, including 20 species and 5 unclassified viroids, as of October 2021, were also introduced. These viruses and viroids are collectively classified into 81 genera within 26 families of 3 kingdoms (Shotokuvirae, Orthornavirae, Pararnavirae) across 2 realms (Monodnaviria and Riboviria). This review also overviewed how Japan’s plant virus/viroid studies have contributed to advance virus/viroid taxonomy.

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  • A novel victorivirus from the phytopathogenic fungus Neofusicoccum parvum Reviewed

    Haris Ahmed Khan, Yukiyo Sato, Hideki Kondo, Atif Jamal, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of Virology   2022.2

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    DOI: 10.1007/s00705-021-05304-7

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  • A new tetra-segmented splipalmivirus with divided RdRP domains from Cryphonectria naterciae, a fungus found on chestnut and cork oak trees in Europe Reviewed

    Yukiyo Sato, Sabitree Shahi, Paul Telengech, Sakae Hisano, Carolina Cornejo, Daniel Rigling, Hideki Kondo, Nobuhiro Suzuki

    Virus Research   307   198606 - 198606   2022.1

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    DOI: 10.1016/j.virusres.2021.198606

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  • Assessment of mycoviral diversity in Pakistani fungal isolates revealed infection by 11 novel viruses of a single strain of Fusarium mangiferae isolate SP1. International journal

    Haris Ahmed Khan, Wajeeha Shamsi, Atif Jamal, Memoona Javaied, Mashal Sadiq, Tehsin Fatma, Aqeel Ahmed, Maleeha Arshad, Mubashra Waseem, Samra Babar, Midhat Mustafa Dogar, Nasar Virk, Hussnain Ahmed Janjua, Hideki Kondo, Nobuhiro Suzuki, Muhammad Faraz Bhatti

    The Journal of general virology   102 ( 12 )   2021.12

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    An extensive screening survey was conducted on Pakistani filamentous fungal isolates for the identification of viral infections. A total of 396 fungal samples were screened, of which 36 isolates were found double-stranded (ds) RNA positive with an overall frequency of 9% when analysed by a classical dsRNA isolation method. One of 36 dsRNA-positive strains, strain SP1 of a plant pathogenic fungus Fusarium mangiferae, was subjected to virome analysis. Next-generation sequencing and subsequent completion of the entire genome sequencing by a classical Sanger sequencing method showed the SP1 strain to be co-infected by 11 distinct viruses, at least seven of which should be described as new taxa at the species level according to the ICTV (International Committee on Taxonomy of Viruses) species demarcation criteria. The newly identified F. mangiferae viruses (FmVs) include two partitivirids, one betapartitivirus (FmPV1) and one gammapartitivirus (FmPV2); six mitovirids, three unuamitovirus (FmMV2, FmMV4, FmMV6), one duamitovirus (FmMV5), and two unclassified mitovirids (FmMV1, FmMV3); and three botourmiavirids, two magoulivirus (FmBOV1, FmBOV3) and one scleroulivirus (FmBOV2). The number of coinfecting viruses is among the largest ones of fungal coinfections. Their molecular features are thoroughly described here. This represents the first large virus survey in the Indian sub-continent.

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  • Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand Reviewed

    Sokty Neang, Santiti Bincader, Sansern Rangsuwan, Pisut Keawmanee, Soriya Rin, Lakha Salaipeth, Subha Das, Hideki Kondo, Nobuhiro Suzuki, Ikuo Sato, Daigo Takemoto, Chainarong Rattanakreetakul, Ratiya Pongpisutta, Masao Arakawa, Sotaro Chiba

    Viruses   13 ( 11 )   2269 - 2269   2021.11

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    DOI: 10.3390/v13112269

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  • A new double-stranded rna mycovirus in cryphonectria naterciae is able to cross the species barrier and is deleterious to a new host Reviewed

    Carolina Cornejo, Sakae Hisano, Helena Bragança, Nobuhiro Suzuki, Daniel Rigling

    Journal of Fungi   7 ( 10 )   861   2021.10

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    DOI: 10.3390/jof7100861

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  • Changes to virus taxonomy and to the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2021) Reviewed

    Peter J. Walker, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Andrew J. Davison, Donald M. Dempsey, Bas E. Dutilh, María Laura García, Balázs Harrach, Robert L. Harrison, R. Curtis Hendrickson, Sandra Junglen, Nick J. Knowles, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Max L. Nibert, Hanna M. Oksanen, Richard J. Orton, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Dooerslaer, Anne-Mieke Vandamme, Arvind Varsani, Francisco Murilo Zerbini

    Archives of Virology   166 ( 9 )   2633 - 2648   2021.9

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    DOI: 10.1007/s00705-021-05156-1

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  • A second capsidless hadakavirus strain with 10 positive-sense single-stranded RNA genomic segments from Fusarium nygamai Reviewed

    Haris Ahmed Khan, Yukiyo Sato, Hideki Kondo, Atif Jamal, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of Virology   166   2711 - 2722   2021.7

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    DOI: 10.1007/s00705-021-05176-x

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  • In-tree behavior of diverse viruses harbored in the chestnut blight fungus, Cryphonectria parasitica Reviewed

    Nobuhiro Suzuki, Carolina Cornejo, Annisa Aulia, Sabitree Shahi, Bradley I. Hillman, Daniel Rigling

    Journal of Virology   95 ( 6 )   e01962-20   2021.3

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    DOI: 10.1128/JVI.01962-20

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  • Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range Reviewed

    Sabitree Shahi, Sotaro Chiba, Hideki Kondo, Nobuhiro Suzuki

    Virology   554   55 - 65   2021.2

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    DOI: 10.1016/j.virol.2020.11.011

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  • Identification of an RNA silencing suppressor encoded by a symptomless fungal hypovirus, Cryphonectria hypovirus 4 Reviewed

    Annisa Aulia, Kiwamu Hyodo, Sakae Hisano, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

    Biology   10 ( 2 )   1 - 16   2021.2

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    DOI: 10.3390/biology10020100

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  • Biochemical characterization of the dicing activity of Dicer-like 2 in the model filamentous fungus Neurospora crassa Reviewed

    Midori Tabara, Hisashi Koiwa, Nobuhiro Suzuki, Toshiyuki Fukuhara

    Fungal Genetics and Biology   146   103488   2021.1

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    DOI: 10.1016/j.fgb.2020.103488

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  • A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix Reviewed

    Juan M. Arjona-López, Paul Telengech, Nobuhiro Suzuki, Carlos J. López-Herrera

    Fungal Biology   125 ( 1 )   69 - 76   2021.1

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    DOI: 10.1016/j.funbio.2020.10.007

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  • Identification of a Novel Quinvirus in the Family Betaflexiviridae That Infects Winter Wheat. Reviewed International journal

    Hideki Kondo, Naoto Yoshida, Miki Fujita, Kazuyuki Maruyama, Kiwamu Hyodo, Hiroshi Hisano, Tetsuo Tamada, Ida Bagus Andika, Nobuhiro Suzuki

    Frontiers in microbiology   12   715545   2021

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    Yellow mosaic disease in winter wheat is usually attributed to the infection by bymoviruses or furoviruses; however, there is still limited information on whether other viral agents are also associated with this disease. To investigate the wheat viromes associated with yellow mosaic disease, we carried out de novo RNA sequencing (RNA-seq) analyses of symptomatic and asymptomatic wheat-leaf samples obtained from a field in Hokkaido, Japan, in 2018 and 2019. The analyses revealed the infection by a novel betaflexivirus, which tentatively named wheat virus Q (WVQ), together with wheat yellow mosaic virus (WYMV, a bymovirus) and northern cereal mosaic virus (a cytorhabdovirus). Basic local alignment search tool (BLAST) analyses showed that the WVQ strains (of which there are at least three) were related to the members of the genus Foveavirus in the subfamily Quinvirinae (family Betaflexiviridae). In the phylogenetic tree, they form a clade distant from that of the foveaviruses, suggesting that WVQ is a member of a novel genus in the Quinvirinae. Laboratory tests confirmed that WVQ, like WYMV, is potentially transmitted through the soil to wheat plants. WVQ was also found to infect rye plants grown in the same field. Moreover, WVQ-derived small interfering RNAs accumulated in the infected wheat plants, indicating that WVQ infection induces antiviral RNA silencing responses. Given its common coexistence with WYMV, the impact of WVQ infection on yellow mosaic disease in the field warrants detailed investigation.

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  • Molecular Characterization of a Novel Polymycovirus From Penicillium janthinellum With a Focus on Its Genome-Associated PASrp Reviewed

    Yukiyo Sato, Atif Jamal, Hideki Kondo, Nobuhiro Suzuki

    Frontiers in Microbiology   11   e0045-20   2020.10

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    DOI: 10.3389/fmicb.2020.592789

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  • Coinfection of Rosellinia necatrix by a partitivirus and a virga-like virus is associated with hypovirulence Reviewed

    Juan M. Arjona-López, Paul Telengech, Nobuhiro Suzuki, Carlos J. López-Herrera

    European Journal of Plant Pathology   158 ( 1 )   111 - 119   2020.9

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    DOI: 10.1007/s10658-020-02058-x

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  • Dicer monitoring in a model filamentous fungus host, Cryphonectria parasitica Reviewed

    Annisa Aulia, Midori Tabara, Paul Telengech, Toshiyuki Fukuhara, Nobuhiro Suzuki

    Current Research in Virological Science   100001 - 100001   2020.7

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    DOI: 10.1016/j.crviro.2020.100001

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  • Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix Reviewed

    Paul Telengech, Sakae Hisano, Cyrus Mugambi, Kiwamu Hyodo, Juan Manuel Arjona-López, Carlos José López-Herrera, Satoko Kanematsu, Hideki Kondo, Nobuhiro Suzuki

    Frontiers in Microbiology   11   1064   2020.6

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  • Virome Analysis of Aphid Populations That Infest the Barley Field: The Discovery of Two Novel Groups of Nege/Kita-Like Viruses and Other Novel RNA Viruses Reviewed International journal

    Hideki Kondo, Miki Fujita, Hiroshi Hisano, Kiwamu Hyodo, Ida Bagus Andika, Nobuhiro Suzuki

    Frontiers in Microbiology   11   509   2020.4

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    DOI: 10.3389/fmicb.2020.00509

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  • Editorial: Frontiers in Fungal Virus Research Reviewed

    Liying Sun, Nobuhiro Suzuki, Daohong Jiang, Massimo Turina, Jiatao Xie

    Frontiers in Cellular and Infection Microbiology   9   456   2020.1

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    DOI: 10.3389/fcimb.2019.00456

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  • Structure and assembly of double-stranded RNA mycoviruses Reviewed

    Carlos P. Mata, Javier M. Rodríguez, Nobuhiro Suzuki, José R. Castón

    Advances in Virus Research   108   213 - 247   2020.1

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    DOI: 10.1016/bs.aivir.2020.08.001

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  • Neo-virology: The raison d'etre of viruses Reviewed International journal

    Tokiko Watanabe, Nobuhiro Suzuki, Keizo Tomonaga, Hirofumi Sawa, Yoshiharu Matsuura, Yasushi Kawaguchi, Hideki Takahashi, Keizo Nagasaki, Yoshihiro Kawaoka

    Virus Research   274   197751 - 197751   2019.12

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    DOI: 10.1016/j.virusres.2019.197751

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  • ICTV Virus Taxonomy Profile: Megabirnaviridae. Reviewed International journal

    Sato, Y, Miyazaki, N, Kanematsu, S, Ghabrial, S. A, Hillman, B. I, Suzuki, N, ICTV Report, Consortium

    Journal of General Virology   100 ( 9 )   269 - 1270   2019.9

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    DOI: 10.1099/jgv.0.001297

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  • Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae Reviewed

    Shuhei Ohkita, Yui Lee, Quyet Nguyen, Kenichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

    Virology   534   25 - 35   2019.8

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    DOI: 10.1016/j.virol.2019.05.015

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  • Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes) Reviewed

    Yu Hsin Lin, Miki Fujita, Sotaro Chiba, Kiwamu Hyodo, Ida Bagus Andika, Nobuhiro Suzuki, Hideki Kondo

    Virology   533   125 - 136   2019.7

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    DOI: 10.1016/j.virol.2019.05.008

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  • Isolation and characterization of a novel mycovirus infecting an edible mushroom, Grifola frondosa Reviewed

    Akiko Komatsu, Hideki Kondo, Masayuki Sato, Atsushi Kurahashi, Kozo Nishibori, Nobuhiro Suzuki, Fumihiro Fujimori

    Mycoscience   60 ( 4 )   211 - 220   2019.7

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    DOI: 10.1016/j.myc.2019.01.005

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  • A symptomless hypovirus, CHV4, facilitates stable infection of the chestnut blight fungus by a coinfecting reovirus likely through suppression of antiviral RNA silencing Reviewed

    Annisa Aulia, Ida Bagus Andika, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

    Virology   533   99 - 107   2019.7

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    DOI: 10.1016/j.virol.2019.05.004

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  • Novel victorivirus from a pakistani isolate of alternaria alternata lacking a typical translational stop/restart sequence signature Reviewed

    Atif Jamal, Yukiyo Sato, Sabitree Shahi, Wajeeha Shamsi, Hideki Kondo, Nobuhiro Suzuki

    Viruses   11 ( 6 )   577   2019.6

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    DOI: 10.3390/v11060577

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  • Molecular and biological characterization of a novel botybirnavirus identified from a Pakistani isolate of Alternaria alternata Reviewed International journal

    Wajeeha Shamsi, Yukiyo Sato, Atif Jamal, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki, Muhammad Faraz Bhatti

    Virus Research   263   119 - 128   2019.4

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    DOI: 10.1016/j.virusres.2019.01.006

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  • A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes. Reviewed

    Kondo H, Chiba S, Maruyama K, Andika IB, Suzuki N

    Virus research   262   37 - 47   2019.3

  • "Integration of viral sequences into eukaryotic host genomes: legacy of ancient infections". Reviewed

    Tomonaga K, Suzuki N, Berkhout B

    Virus research   262   1   2019.3

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    DOI: 10.1016/j.virusres.2018.12.012

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  • Hijacking a host scaffold protein, RACK1, for replication of a plant RNA virus. Reviewed

    Hyodo K, Suzuki N, Okuno T

    The New phytologist   221 ( 2 )   935 - 945   2019.1

  • Investigation of Host Range of and Host Defense against a Mitochondrially Replicating Mitovirus Reviewed

    Sabitree Shahi, Ana Eusebio-Cope, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

    Journal of Virology   93 ( 6 )   2019.1

  • The biological attributes, genome architecture and packaging of diverse multi-component fungal viruses Reviewed International journal

    Yukiyo Sato, Jos{\'{e, R Cast{\'{o } }n, Nobuhiro Suzuki

    Current Opinion in Virology   33   55 - 65   2018.12

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    DOI: 10.1016/j.coviro.2018.07.009

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  • ICTV Virus Taxonomy Profile: Quadriviridae. Reviewed

    Chiba S, Castón JR, Ghabrial SA, Suzuki N, Ictv Report, Consortium

    The Journal of general virology   99 ( 11 )   1480 - 1481   2018.11

  • Novel Mitoviruses and a Unique Tymo-Like Virus in Hypovirulent and Virulent Strains of the Fusarium Head Blight Fungus, Fusarium boothii Reviewed

    Mizutani Y, Abraham A, Uesaka K, Kondo H, Suga H, Suzuki N, Chiba S

    Viruses   10 ( 11 )   2018.10

  • Capsid Structure of dsRNA Fungal Viruses. Reviewed

    Luque D, Mata CP, Suzuki N, Ghabrial SA, Castón JR

    Viruses   10 ( 9 )   2018.9

  • ICTV virus taxonomy profile: Hypoviridae Reviewed

    Nobuhiro Suzuki, Said A. Ghabrial, Kook-Hyung Kim, Michael Pearson, Shin-Yi L. Marzano, Hajime Yaegashi, Jiatao Xie, Lihua Guo, Hideki Kondo, Igor Koloniuk, Bradley I. Hillman, Elliot J. Lefkowitz, Andrew J. Davison, Stuart G. Siddell, Sead Sabanadzovic, Donald B. Smith, Richard J. Orton, Peter Simmonds, ICTV Report Consortium

    Journal of General Virology   99 ( 5 )   615 - 616   2018.5

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  • Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus, Rosellinia necatrix: insights into evolutionary biology of fungal viruses Reviewed

    Juan Manuel Arjona-Lopez, Paul Telengech, Atif Jamal, Sakae Hisano, Hideki Kondo, Mery Dafny Yelin, Isabel Arjona-Girona, Satoko Kanematsu, Carlos José Lopez-Herrera, Nobuhiro Suzuki

    Environmental Microbiology   20 ( 4 )   1464 - 1483   2018.4

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  • Erratum: A fungal Argonaute interferes with RNA interference (Nucleic acids research (2018) 46 5 (2495-2508))

    Quyet Nguyen, Akihide Iritani, Shuhei Ohkita, Ba V. Vu, Kana Yokoya, Ai Matsubara, Ken-Ichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

    Nucleic acids research   46 ( 5 )   2698   2018.3

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  • First evidence for internal ribosomal entry sites in diverse fungal virus genomes Reviewed

    Sotaro Chiba, Atif Jamal, Nobuhiro Suzuki

    mBio   9 ( 2 )   2018.3

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    DOI: 10.1128/mBio.02350-17

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  • A fungal Argonaute interferes with RNA interference. Reviewed International journal

    Nguyen Q, Iritani A, Ohkita S, Vu BV, Yokoya K, Matsubara A, Ikeda KI, Suzuki N, Nakayashiki H

    Nucleic acids research   46 ( 5 )   2495 - 2508   2018.3

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    Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein-sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2, one of the three AGO genes in the fungus Pyricularia oryzae (Magnaporthe oryzae) interferes with RNAi. Gene knockout (KO) studies revealed that MoAGO1 and MoAGO3 additively or redundantly played roles in hairpin RNA- and retrotransposon (MAGGY)-triggered RNAi while, surprisingly, the KO mutants of MoAGO2 (Δmoago2) showed elevated levels of gene silencing. Consistently, transcript levels of MAGGY and mycoviruses were drastically reduced in Δmoago2, supporting the idea that MoAGO2 impeded RNAi against the parasitic elements. Deep sequencing analysis revealed that repeat- and mycovirus-derived small interfering RNAs were mainly associated with MoAGO2 and MoAGO3, and their populations were very similar based on their size distribution patterns and positional base preference. Site-directed mutagenesis studies indicated that sRNA binding but not slicer activity of MoAGO2 was essential for the ability to diminish the efficacy of RNAi. Overall, these results suggest a possible interplay between distinct sRNA-mediated gene regulation pathways through a competition for sRNA.

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  • A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in an unrelated dsRNA virus: Taxonomic and evolutionary considerations Reviewed

    Sakae Hisano, Rui Zhang, Md. Iqbal Faruk, Hideki Kondo, Nobuhiro Suzuki

    Virus Research   244   75 - 83   2018.1

  • ICTV virus taxonomy profile: Partitiviridae Reviewed

    Eeva J. Vainio, Sotaro Chiba, Said A. Ghabrial, Edgar Maiss, Marilyn Roossinck, Sead Sabanadzovic, Nobuhiro Suzuki, Jiatao Xie, Max Nibert, Elliot J. Lefkowitz, Andrew J. Davison, Stuart G. Siddell, Donald B. Smith, Richard J. Orton, Peter Simmonds, ICTV Report Consortium

    Journal of General Virology   99 ( 1 )   17 - 18   2018.1

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    DOI: 10.1099/jgv.0.000985

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  • Viruses of Plant-Interacting Fungi Reviewed

    Bradley I. Hillman, Aulia Annisa, Nobuhiro Suzuki

    Advances in Virus Research   100   99 - 116   2018.1

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    DOI: 10.1016/bs.aivir.2017.10.003

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  • Acquisition of functions on the outer capsid surface during evolution of double-stranded RNA fungal viruses Reviewed

    Carlos P. Mata, Daniel Luque, Josue Gomez-Blanco, Javier M. Rodriguez, Jose M. Gonzalez, Nobuhiro Suzuki, Said A. Ghabrial, Jose L. Carrascosa, Benes L. Truss, Jose R. Caston

    PLOS PATHOGENS   13 ( 12 )   e1006755   2017.12

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  • A possible occurrence of genome reassortment among bipartite rhabdoviruses Reviewed

    Hideki Kondo, Keisuke Hirota, Kazuyuki Maruyama, Ida Bagus An'dika, Nobuhiro Suzuki

    VIROLOGY   508   18 - 25   2017.8

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    DOI: 10.1016/j.virol.2017.04.027

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  • Roles of superoxide anion and hydrogen peroxide during replication of two unrelated plant RNA viruses in Nicotiana benthamiana Reviewed

    Kiwamu Hyodo, Nobuhiro Suzuki, Kazuyuki Mise, Tetsuro Okuno

    Plant Signaling and Behavior   12 ( 6 )   e1338223   2017.6

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  • Heterodimers as the Structural Unit of the T=1 Capsid of the Fungal Double-Stranded RNA Rosellinia necatrix Quadrivirus 1 Reviewed

    Daniel Luque, Carlos P. Mata, Fernando Gonzalez-Camacho, Jose M. Gonzalez, Josue Gomez-Blanco, Carlos Alfonso, German Rivas, Wendy M. Havens, Satoko Kanematsu, Nobuhiro Suzuki, Said A. Ghabrial, Benes L. Trus, Jose R. Caston

    JOURNAL OF VIROLOGY   90 ( 24 )   11220 - 11230   2016.12

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  • Reprint of “Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi” Reviewed

    Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki

    Virus Research   219   39 - 50   2016.7

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    DOI: 10.1016/j.virusres.2016.05.011

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  • The world of diverse viruses in the kingdom Fungi Foreword Reviewed

    Nobuhiro Suzuki

    VIRUS RESEARCH   219   1 - 1   2016.7

  • Characterization of a new megabirnavirus that confers hypovirulence with the aid of a co-infecting partitivirus to the host fungus, Rosellinia necatrix Reviewed

    Atsuko Sasaki, Hitoshi Nakamura, Nobuhiro Suzuki, Satoko Kanernatsu

    VIRUS RESEARCH   219   73 - 82   2016.7

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  • A novel betapartitivirus RnPV6 from Rosellinia necatrix tolerates host RNA silencing but is interfered by its defective RNAs Reviewed

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    VIRUS RESEARCH   219   62 - 72   2016.7

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  • The victorivirus Helminthosporium victoriae virus 1905 is the primary cause of disease/hypovirulence in its natural host and a heterologous host (Reprinted from Virus Res., vol 213, pg 238-245, 2016) Reviewed

    Jiatao Xie, Wendy M. Havens, Yu-Hsin Lin, Nobuhiro Suzuki, Said A. Ghabrial

    VIRUS RESEARCH   219   100 - 107   2016.7

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  • Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi Reviewed

    Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki

    VIRUS RESEARCH   213   353 - 364   2016.2

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  • The victorivirus Helminthosporium victoriae virus 190S is the primary cause of disease/hypovirulence in its natural host and a heterologous host Reviewed

    Jiatao Xie, Wendy M. Havens, Yu-Hsin Lin, Nobuhiro Suzuki, Said A. Ghabrial

    VIRUS RESEARCH   213   238 - 245   2016.2

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  • Megabirnavirus structure reveals a putative 120-subunit capsid formed by asymmetrical dimers with distinctive large protrusions Reviewed

    Naoyuki Miyazaki, Lakha Salaipeth, Satoko Kanematsu, Kenji Iwasaki, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   96 ( 8 )   2435 - 2441   2015.8

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  • Different Dicer-like protein components required for intracellular and systemic antiviral silencing in Arabidopsis thaliana Reviewed

    Ida Bagus Andika, Kazuyuki Maruyama, Liying Sun, Hideki Kondo, Tetsuo Tamada, Nobuhiro Suzuki

    PLANT SIGNALING & BEHAVIOR   10 ( 8 )   e1039214   2015.8

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  • Cymbidium chlorotic mosaic virus, a new sobemovirus isolated from a spring orchid (Cymbidium goeringii) in Japan Reviewed

    Hideki Kondo, Shogo Takemoto, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY   160 ( 8 )   2099 - 2104   2015.8

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  • Large-scale multiplex polymerase chain reaction assay for diagnosis of viral reactivations after allogeneic hematopoietic stem cell transplantation Reviewed

    Natsuko Inazawa, Tsukasa Hori, Naoki Hatakeyama, Masaki Yamamoto, Yuko Yoto, Masanori Nojima, Nobuhiro Suzuki, Norio Shimizu, Hiroyuki Tsutsumi

    JOURNAL OF MEDICAL VIROLOGY   87 ( 8 )   1427 - 1435   2015.8

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  • 50-plus years of fungal viruses Reviewed

    Said A. Ghabrial, Jose R. Caston, Daohong Jiang, Max L. Nibert, Nobuhiro Suzuki

    VIROLOGY   479   356 - 368   2015.5

  • Editorial: Viruses threatening stable production of cereal crops Reviewed

    Nobuhiro Suzuki, Takahide Sasaya, Il-Ryong Choi

    FRONTIERS IN MICROBIOLOGY   6   470   2015.5

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  • The chestnut blight fungus for studies on virus/host and virus/virus interactions: From a natural to a model host Reviewed

    Ana Eusebio-Cope, Liying Sun, Toru Tanaka, Sotaro Chiba, Shin Kasahara, Nobuhiro Suzuki

    VIROLOGY   477   164 - 175   2015.3

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    DOI: 10.1016/j.virol.2014.09.024

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  • Differential contributions of plant Dicer-like proteins to antiviral defences against potato virus X in leaves and roots Reviewed

    Ida Bagus Andika, Kazuyuki Maruyama, Liying Sun, Hideki Kondo, Tetsuo Tamada, Nobuhiro Suzuki

    PLANT JOURNAL   81 ( 5 )   781 - 793   2015.3

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    DOI: 10.1111/tpj.12770

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  • Detection and analysis of non-retroviral RNA virus-like elements in plant, fungal, and insect genomes. Reviewed International journal

    Hideki Kondo, Sotaro Chiba, Nobuhiro Suzuki

    Methods in molecular biology (Clifton, N.J.)   1236   73 - 88   2015

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    Endogenous non-retroviral RNA like sequences (NRVSs) have been discovered in the genome of a wide range of eukaryotes. These are considered as fossil RNA viral elements integrated into host genomes by as-yet-known mechanisms, and in many cases, those fossils are estimated to be millions-of-years-old. It is likely that the number of NRVS records will increase rapidly due to the growing availability of whole-genome sequences for many kinds of eukaryotes. Discovery of the novel NRVSs and understanding of their phylogenetic relationship with modern viral relatives provide important information on deep evolutionary history of RNA virus-host interactions. In this chapter, therefore, the common strategies for the identification and characterization of endogenous NRVSs from plants, insects, and fungi are described.

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  • Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research Reviewed

    Max L. Nibert, Said A. Ghabrial, Edgar Maiss, Till Lesker, Eeva J. Vainio, Daohong Jiang, Nobuhiro Suzuki

    VIRUS RESEARCH   188   128 - 141   2014.8

  • A novel single-stranded RNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix, with similarity to hypo-like viruses Reviewed

    Rui Zhang, Shengxue Liu, Sotaro Chiba, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    FRONTIERS IN MICROBIOLOGY   5   360   2014.7

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  • Biological properties and expression strategy of rosellinia necatrix megabirnavirus 1 analysed in an experimental host, Cryphonectria parasitica Reviewed

    Lakha Salaipeth, Sotaro Chiba, Ana Eusebio-Cope, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   95 ( Pt 3 )   740 - 750   2014.3

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  • Transcriptional mapping of the messenger and leader RNAs of orchid fleck virus, a bisegmented negative-strand RNA virus Reviewed

    Hideki Kondo, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    VIROLOGY   452   166 - 174   2014.3

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  • Genome rearrangement of a mycovirus Rosellinia necatrix megabirnavirus 1 affecting its ability to attenuate virulence of the host fungus Reviewed

    Satoko Kanematsu, Takeo Shimizu, Lakha Salaipeth, Hajime Yaegashi, Atsuko Sasaki, Tsutae Ito, Nobuhiro Suzuki

    VIROLOGY   450   308 - 315   2014.2

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    DOI: 10.1016/j.virol.2013.12.002

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  • Complete genome sequence of Habenaria mosaic virus, a new potyvirus infecting a terrestrial orchid (Habenaria radiata) in Japan Reviewed

    Hideki Kondo, Takanori Maeda, I. Wayan Gara, Sotaro Chiba, Kazuyuki Maruyama, Tetsuo Tamada, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY   159 ( 1 )   163 - 166   2014.1

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  • [Cryphonectria parasitica as a host of fungal viruses: a tool useful to unravel the mycovirus world]. Reviewed

    Suzuki N

    Uirusu   64 ( 1 )   11 - 24   2014

  • [Diverse double-stranded RNA viruses infecting fungi]. Reviewed

    Chiba S, Suzuki N

    Uirusu   64 ( 2 )   225 - 238   2014

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    Most of reported fungal viruses (mycoviruses) have double-stranded RNA (dsRNA) genomes. This may reflect the simple, easy method for mycovirus hunting that entails detection of dsRNAs as a sign of viral infections. There are an increasing number of screens of various fungi, particularly phytopathogenic fungi for viruses pathogenic to host fungi or able to confer hypovirulence to them. This bases on an attractive research field of biological control of fungal plant diseases using viruses (virocontrol), mainly targeting important phytopathogenic fungi. While isolated viruses usually induce asymptomatic symptoms, they show a considerably high level of diversity. As of 2014, fungal dsRNA viruses are classified into six families: Reoviridae, Totiviridae, Chrysoviridae, Partitiviridae, Megabirnaviridae and Quadriviridae. These exclude unassigned mycoviruses which will definitely be placed into distinct families and/or genera. In this review article, dsRNA viruses isolated from the kingdom Fungi including as-yet-unclassified taxa are overviewed. Some recent achievements in the related field are briefly introduced as well.

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  • Nyamiviridae: Proposal for a new family in the order Mononegavirales Reviewed

    Jens H. Kuhn, Sadia Bekal, Yingyun Cai, Anna N. Clawson, Leslie L. Domier, Marieke Herrel, Peter B. Jahrling, Hideki Kondo, Kris N. Lambert, Kathie A. Mihindukulasuriya, Norbert Nowotny, Sheli R. Radoshitzky, Urs Schneider, Peter Staeheli, Nobuhiro Suzuki, Robert B. Tesh, David Wang, Lin-Fa Wang, Ralf G. Dietzgen

    ARCHIVES OF VIROLOGY   158 ( 10 )   2209 - 2226   2013.10

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  • Orchid fleck virus structural proteins N and P form intranuclear viroplasm like structures in the absence of viral infection Reviewed

    Hideki Kondo, Sotaro Chiba, Ida Bagus Andika, Kazuyuki Maruyama, Tetsuo Tamada, Nobuhiro Suzuki

    Journal of Virology   87 ( 13 )   7423 - 7434   2013.7

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  • A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia necatrix, Is Infectious as Particles and Targeted by RNA Silencing Reviewed

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   87 ( 12 )   6727 - 6738   2013.6

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  • A second quadrivirus strain from the phytopathogenic filamentous fungus Rosellinia necatrix Reviewed

    Yu-Hsin Lin, Sakae Hisano, Hajime Yaegashi, Satoko Kanematsu, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY   158 ( 5 )   1093 - 1098   2013.5

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  • Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system Reviewed

    Zoe Sanchez, Akio Tani, Nobuhiro Suzuki, Reiko Kariyama, Hiromi Kumon, Kazuhide Kimbara

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   115 ( 3 )   326 - 331   2013.3

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  • Effects of Defective Interfering RNA on Symptom Induction by, and Replication of, a Novel Partitivirus from a Phytopathogenic Fungus, Rosellinia necatrix Reviewed

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   87 ( 4 )   2330 - 2341   2013.2

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  • Evidence for negative-strand RNA virus infection in fungi Reviewed

    Hideki Kondo, Sotaro Chiba, Kazuhiro Toyoda, Nobuhiro Suzuki

    Virology   435 ( 2 )   201 - 209   2013.1

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    DOI: 10.1016/j.virol.2012.10.002

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  • Viruses of the white root rot fungus, Rosellinia necatrix. Reviewed International journal

    Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    Advances in virus research   86   177 - 214   2013

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    DOI: 10.1016/B978-0-12-394315-6.00007-6

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  • A novel quadripartite dsRNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix Reviewed

    Yu-Hsin Lin, Sotaro Chiba, Akio Tani, Hideki Kondo, Atsuko Sasaki, Satoko Kanematsu, Nobuhiro Suzuki

    VIROLOGY   426 ( 1 )   42 - 50   2012.4

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  • Mycoreovirus genome alterations: similarities to and differences from rearrangements reported for other reoviruses Reviewed

    Toru Tanaka, Ana Eusebio-Cope, Liying Sun, Nobuhiro Suzuki

    FRONTIERS IN MICROBIOLOGY   3   186   2012

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  • Rearrangements of mycoreovirus 1 S1, S2 and S3 induced by the multifunctional protein p29 encoded by the prototypic hypovirus Cryphonectria hypovirus 1 strain EP713 Reviewed

    Toru Tanaka, Liying Sun, Kouhei Tsutani, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   92 ( Pt 8 )   1949 - 1959   2011.8

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    DOI: 10.1099/vir.0.031138-0

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  • Widespread endogenization of genome sequences of non-retroviral rna viruses into plant genomes

    Sotaro Chiba, Hideki Kondo, Akio Tani, Daisuke Saisho, Wataru Sakamoto, Satoko Kanematsu, Nobuhiro Suzuki

    PLoS Pathogens   7 ( 7 )   2011.7

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  • Rice Dwarf Viruses with Dysfunctional Genomes Generated in Plants Are Filtered Out in Vector Insects: Implications for the Origin of the Virus Reviewed

    Yingying Pu, Akira Kikuchi, Yusuke Moriyasu, Masatoshi Tomaru, Yan Jin, Haruhisa Suga, Kyoji Hagiwara, Fusamichi Akita, Takumi Shimizu, Osamu Netsu, Nobuhiro Suzuki, Tamaki Uehara-Ichiki, Takahide Sasaya, Taiyun Wei, Yi Li, Toshihiro Omura

    JOURNAL OF VIROLOGY   85 ( 6 )   2975 - 2979   2011.3

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  • [Mycoviruses and virocontrol]. Reviewed

    Chiba S, Kondo H, Kanematsu S, Suzuki N

    Uirusu   60 ( 2 )   163 - 176   2010.12

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    Viruses are widespread in all major groups of fungi. The transmission of fungal viruses occurs intracellularly during cell division, sporogenesis, and cell fusion. They apparently lack an extracellular route for infection. Recent searches of the collections of field fungal isolates have detected an increasing number of novel viruses and lead to discoveries of novel genome organizations, expression strategies and virion structures. Those findings enhanced our understanding of virus diversity and evolution. The majority of fungal viruses have dsRNA genomes packaged in spherical particles, while ssRNA mycoviruses, possessing or lacking the ability to form particles, have increasingly been reported. This review article discusses the current status of mycovirus studies and virocontrol (biocontrol) of phytopathogenic fungi using viruses that infect them and reduce their virulence. Selected examples of virocontrol-associated systems include the chestnut/chestnut blight/hypovirus and fruit trees/white root rot fungus/mycoviruses. Natural dissemination and artificial introduction of hypovirulent fungal strains efficiently contributed to virocontrol of chestnut blight in European forests. Attempts to control white root rot with hypovirulence-conferring mycoviruses are now being made in Japan.

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  • Complexity in Influenza Virus Targeted Drug Design: Interaction with Human Sialidases Reviewed

    Leonard M. G. Chavas, Ryuichi Kato, Nobuhiro Suzuki, Mark von Itzstein, Maretta C. Mann, Robin J. Thomson, Jeffrey C. Dyason, Jennifer McKimm-Breschkin, Paola Fusi, Cristina Tringali, Bruno Venerando, Guido Tettamanti, Eugenio Monti, Soichi Wakatsuki

    JOURNAL OF MEDICINAL CHEMISTRY   53 ( 7 )   2998 - 3002   2010.4

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  • Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction Reviewed

    Ana Eusebio-Cope, Liying Sun, Bradley I. Hillman, Nobuhiro Suzuki

    VIROLOGY   397 ( 2 )   399 - 408   2010.2

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  • Overexpression of microRNA395c or 395e affects differently the seed germination of arabidopsis thaliana under stress conditions Reviewed

    Joo Yeol Kim, Hwa Jung Lee, Hyun Ju Jung, Kazuyuki Maruyama, Nobuhiro Suzuki, Hunseung Kang

    Planta   232 ( 6 )   1447 - 1454   2010

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    DOI: 10.1007/s00425-010-1267-x

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  • A Novel Bipartite Double-Stranded RNA Mycovirus from the White Root Rot Fungus Rosellinia necatrix: Molecular and Biological Characterization, Taxonomic Considerations, and Potential for Biological Control Reviewed

    Sotaro Chiba, Lakha Salaipeth, Yu-Hsin Lin, Atsuko Sasaki, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   83 ( 24 )   12801 - 12812   2009.12

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    DOI: 10.1128/JVI.01830-09

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  • Characteristics of Hemophagocytic Lymphohistiocytosis in Neonates: A Nationwide Survey in Japan Reviewed

    Nobuhiro Suzuki, Akira Morimoto, Shouichi Ohga, Kazuko Kudo, Yasushi Ishida, Eiichi Ishii

    JOURNAL OF PEDIATRICS   155 ( 2 )   235 - 238   2009.8

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    DOI: 10.1016/j.jpeds.2009.02.050

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  • Occurrence of the African subgroup (Ia) of BK polyomavirus in younger Japanese children Reviewed

    Kaori Tanaka, Tsukasa Hori, Naoki Hatakeyama, Masaki Yamamoto, Rumiko Takayama, Yuko Yoto, Nobuhiro Suzuki, Toshiaki Hayashi, Yukiho Ikeda, Hiroshi Ikeda, Tadao Ishida, Hiroyuki Tsutsumi

    MICROBIOLOGY AND IMMUNOLOGY   53 ( 6 )   319 - 322   2009.6

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    DOI: 10.1111/j.1348-0421.2009.00129.x

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  • Coupled termination/reinitiation for translation of the downstream open reading frame B of the prototypic hypovirus CHV1-EP713 Reviewed

    Li-hua Guo, Liying Sun, Sotaro Chiba, Hiroyuki Araki, Nobuhiro Suzuki

    NUCLEIC ACIDS RESEARCH   37 ( 11 )   3645 - 3659   2009.6

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    DOI: 10.1093/nar/gkp224

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  • Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica Reviewed

    Ana Eusebio-Cope, Nobuhiro Suzuki, Hamid Sadeghi-Garmaroodi, Masatoki Taga

    FUNGAL GENETICS AND BIOLOGY   46 ( 4 )   342 - 351   2009.4

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    DOI: 10.1016/j.fgb.2009.01.005

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  • Intragenic rearrangements of a mycoreovirus induced by the multifunctional protein p29 encoded by the prototypic hypovirus CHV1-EP713 Reviewed

    Liying Sun, Nobuhiro Suzuki

    RNA-A PUBLICATION OF THE RNA SOCIETY   14 ( 12 )   2557 - 2571   2008.12

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    DOI: 10.1261/rna.1125408

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  • Characterization of mutants of the chestnut blight fungus (Cryphonectria parasitica) with unusual hypovirus symptoms Reviewed

    Md. Iqbal Faruk, Masatoshi Izumimoto, Nobuhiro Suzuki

    JOURNAL OF GENERAL PLANT PATHOLOGY   74 ( 6 )   425 - 433   2008.12

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    DOI: 10.1007/s10327-008-0121-5

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  • A host factor involved in hypovirus symptom expression in the chestnut blight fungus, Cryphonectria parasitica Reviewed

    M. Iqbal Faruk, Ana Eusebio-Cope, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   82 ( 2 )   740 - 754   2008.1

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  • Baculovirus expression of the 11 mycoreovirus-1 genome segments and identification of the guanylyltransferase-encoding segment

    S. Supyani, Bradley I. Hillman, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   88   342 - 350   2007.1

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    DOI: 10.1099/vir.0.82318-0

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  • Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713 Reviewed

    Liying Sun, Donald L. Nuss, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   87   3703 - 3714   2006.12

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    DOI: 10.1099/vir0.82213-0

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  • Pns4 of rice dwarf virus is a phosphoprotein, is localized around the viroplasm matrix, and forms minitubules Reviewed

    T. Wei, A. Kikuchi, N. Suzuki, T. Shimizu, K. Hagiwara, H. Chen, T. Omura

    Archives of Virology   151 ( 9 )   1701 - 1712   2006.9

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    DOI: 10.1007/s00705-006-0757-4

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  • The spread of Rice dwarf virus among cells of its insect vector exploits virus-induced tubular structures Reviewed

    Taiyun Wei, Akira Kikuchi, Yusuke Moriyasu, Nobuhiro Suzuki, Takumi Shimizu, Kyoji Hagiwara, Hongyan Chen, Mami Takahashi, Tamaki Ichiki-Uehara, Toshihiro Omura

    JOURNAL OF VIROLOGY   80 ( 17 )   8593 - 8602   2006.9

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    DOI: 10.1128/JVI.00537-06

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  • Pns12 protein of Rice dwarf virus is essential for formation of viroplasms and nucleation of viral-assembly complexes Reviewed

    Taiyun Wei, Takumi Shimizu, Kyoji Hagiwara, Akira Kikuchi, Yusuke Moriyasu, Nobuhiro Suzuki, Hongyan Chen, Toshihiro Omura

    Journal of General Virology   87 ( 2 )   429 - 438   2006.2

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    DOI: 10.1099/vir.0.81425-0

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  • Complete genome sequence of Mycoreovirus-1/Cp9B21, a member of a novel genus within the family Reoviridae, isolated from the chestnut blight fungus Cryphonectria parasitica Reviewed

    N Suzuki, S Supyani, K Maruyama, BI Hillman

    JOURNAL OF GENERAL VIROLOGY   85   3437 - 3448   2004.11

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    DOI: 10.1099/vir.0.80293-0

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  • A reovirus of the fungus Cryphonectria parasitica that is infectious as particles and related to the Coltivirus genus of animal pathogens Reviewed

    BI Hillman, S Supyani, H Kondo, N Suzuki

    JOURNAL OF VIROLOGY   78 ( 2 )   892 - 898   2004.1

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    DOI: 10.1128/JVI.78.2.892-898.2004

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  • Viruses of the Chestnut Blight Fungus, Cryphonectria parasitica

    Bradley I. Hillman, Nobuhiro Suzuki

    Advances in Virus Research   63   423 - 472   2004

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    DOI: 10.1016/S0065-3527(04)63007-7

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  • Hypovirus papain-like protease p29 functions in trans to enhance viral double-stranded RNA accumulation and vertical transmission

    N Suzuki, K Maruyama, M Moriyama, DL Nuss

    JOURNAL OF VIROLOGY   77 ( 21 )   11697 - 11707   2003.11

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    DOI: 10.1128/JVI.77.21.11697-11707.2003

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  • Extending chestnut blight hypovirus host range within diaporthales by biolistic delivery of viral cDNA Reviewed

    A Sasaki, M Onoue, S Kanematsu, K Suzaki, M Miyanishi, N Suzuki, DL Nuss, K Yoshida

    MOLECULAR PLANT-MICROBE INTERACTIONS   15 ( 8 )   780 - 789   2002.8

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    DOI: 10.1094/MPMI.2002.15.8.780

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  • Contribution of protein p40 to hypovirus-mediated modulation of fungal host phenotype and viral RNA accumulation

    N Suzuki, DL Nuss

    JOURNAL OF VIROLOGY   76 ( 15 )   7747 - 7759   2002.8

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    DOI: 10.1128/JVI.76.15.7747-7759.2002

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  • Engineering hypoviruses for fundamental and practical applications

    Donald L. Nuss, Baoshan Chen, Lynn M. Geletka, Todd B. Parsley, Nobuhiro Suzuki

    dsRNA Genetic Elements: Concepts and Applications in Agriculture, Forestry, and Medicine   145 - 163   2001.1

  • Essential and dispensable virus-encoded replication elements revealed by efforts to develop hypoviruses as gene expression vectors

    N Suzuki, LM Geletka, DL Nuss

    JOURNAL OF VIROLOGY   74 ( 16 )   7568 - 7577   2000.8

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    DOI: 10.1128/JVI.74.16.7568-7577.2000

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  • Similarity and divergence among viruses in the genus Furovirus

    Y Shirako, N Suzuki, RC French

    VIROLOGY   270 ( 1 )   201 - 207   2000.4

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    DOI: 10.1006/viro.2000.0251

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  • Mapping of a hypovirus p29 protease symptom determinant domain with sequence similarity to potyvirus HC-Pro protease

    Nobuhiro Suzuki, Baoshan Chen, Donald L. Nuss

    Journal of Virology   73   9478 - 9484   1999.10

  • In vivo and in vitro phosphorylation of rice dwarf phytoreovirus Pns12 cytoplasmic nonstructural protein

    N Suzuki, D Hosokawa, Y Matsuura, A Kikuchi, T Omura

    ARCHIVES OF VIROLOGY   144 ( 7 )   1371 - 1380   1999

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    DOI: 10.1007/s007050050593

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  • The loss of outer capsid protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovirus

    Masatoshi Tomaru, Wakako Maruyama, Akira Kikuchi, Jin Yan, Yafeng Zhu, Nobuhiro Suzuki, Masamichi Isogai, Yuzuru Oguma, Ikuo Kimura, Toshihiro Omura

    Journal of Virology   71 ( 10 )   8019 - 8023   1997

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  • Polycistronic (tri- or bicistronic) phytoreoviral segments translatable in both plant and insect cells

    N Suzuki, M Sugawara, DL Nuss, Y Matsuura

    JOURNAL OF VIROLOGY   70 ( 11 )   8155 - 8159   1996.11

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  • Novel NTP binding property of rice dwarf phytoreovirus minor core protein P5

    N Suzuki, T Kusano, Y Matsuura, T Omura

    VIROLOGY   219 ( 2 )   471 - 474   1996.5

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    DOI: 10.1006/viro.1996.0273

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  • DNA cloning, sequence analysis of segment 7 of rice dwarf virus genome and its high expression in E. coli.(共著) Reviewed

    Qu, L, Li, Y, Quan, S, Ding, S, Suzuki, N, Chen, Z

    Acta Microbiologica Sinica   36   335 - 343   1996

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  • A MAIZE DNA-BINDING FACTOR WITH A BZIP MOTIF IS INDUCED BY LOW-TEMPERATURE

    T KUSANO, T BERBERICH, M HARADA, N SUZUKI, K SUGAWARA

    MOLECULAR & GENERAL GENETICS   248 ( 5 )   507 - 517   1995.9

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    DOI: 10.1007/BF02423445

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  • Molecular Analysis of the Rice Dwarf Phytoreovirus Genome

    SUZUKI Nobuhiro

    Annals of the Phytopathological Society of Japan   61 ( 3 )   176 - 176   1995.6

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    DOI: 10.3186/jjphytopath.61.176

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  • Molecular analysis of the rice dwarf virus genome

    Nobuhiro Suzuki

    Seminars in Virology   6 ( 2 )   89 - 95   1995

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  • NUCLEOTIDE-SEQUENCE OF RICE DWARF PHYTOREOVIRUS GENOME SEGMENT-2 - COMPLETION OF SEQUENCE ANALYSES OF RICE DWARF VIRUS

    UYEDA, I, N SUDA, N YAMADA, H KUDO, K MURAO, H SUGA, KIMURA, I, E SHIKATA, Y KITAGAWA, T KUSANO, M SUGAWARA, N SUZUKI

    INTERVIROLOGY   37 ( 1 )   6 - 11   1994.1

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    DOI: 10.1159/000150348

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  • Immunodetection of Rice Dwarf Phytoreoviral Proteins in Both Insect and Plant Hosts

    Nobuhiro Suzuki, Mikiko Sugawara, Tomonobu Kusano, Hajime Mori, Yoshiharu Matsuura

    Virology   202 ( 1 )   41 - 48   1994

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    DOI: 10.1006/viro.1994.1320

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  • LOW-TEMPERATURE-DEPENDENT EXPRESSION OF A RICE GENE ENCODING A PROTEIN WITH A LEUCINE-ZIPPER MOTIF

    K AGUAN, K SUGAWARA, N SUZUKI, T KUSANO

    MOLECULAR & GENERAL GENETICS   240 ( 1 )   1 - 8   1993.7

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    DOI: 10.1007/BF00276876

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  • Molecular analysis of rice dwarf phytoreovirus segment S1: Interviral homology of the putative RNA-dependent RNA polymerase between plant- and animal-infecting reoviruses

    Nobuhiro Suzuki, Masako Tanimura, Yuzuko Watanabe, Tomonobu Kusano, Yoshichika Kitagawa, Narushi Suda, Hiroshi Kudo, Ichiro Uyeda, Eishiro Shikata

    Virology   190 ( 1 )   240 - 247   1992

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    DOI: 10.1016/0042-6822(92)91210-L

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  • Rice dwarf phytoreovirus segment S12 transcript is tricistronic in Vitro

    Nobuhiro Suzuki, Mikiko Sugawara, Tomonobu Kusano

    Virology   191 ( 2 )   992 - 995   1992

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    DOI: 10.1016/0042-6822(92)90279-X

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  • ISOLATION OF GENES FOR LOW-TEMPERATURE-INDUCED PROTEINS IN RICE BY A SIMPLE SUBTRACTIVE METHOD

    K AGUAN, K SUGAWARA, N SUZUKI, T KUSANO

    PLANT AND CELL PHYSIOLOGY   32 ( 8 )   1285 - 1289   1991.12

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  • MOLECULAR ANALYSIS OF RICE DWARF PHYTOREOVIRUS SEGMENT-S11 CORRESPONDING TO WOUND TUMOR PHYTOREOVIRUS SEGMENT-S12 Reviewed

    N SUZUKI, M HARADA, T KUSANO

    JOURNAL OF GENERAL VIROLOGY   72   2233 - 2237   1991.9

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  • MOLECULAR-CLONING AND EXPRESSION OF THIOBACILLUS-FERROOXIDANS CHROMOSOMAL RIBULOSE BISPHOSPHATE CARBOXYLASE GENES IN ESCHERICHIA-COLI

    T KUSANO, K SUGAWARA, C INOUE, N SUZUKI

    CURRENT MICROBIOLOGY   22 ( 1 )   35 - 41   1991.1

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    DOI: 10.1007/BF02106210

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  • Outer capsid protein heterogeneity of rice dwarf phytoreovirus

    N. Suzuki, M. Sugawara

    Journal of General Virology   72 ( 9 )   2239 - 2242   1991

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    DOI: 10.1099/0022-1317-72-9-2239

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  • Molecular analysis of rice dwarf phytoreovirus segment S11 corresponding to wound tumour phytoreovirus segment S12

    N. Suzuki, M. Harada, T. Kusano

    Journal of General Virology   72 ( 9 )   2233 - 2237   1991

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    DOI: 10.1099/0022-1317-72-9-2233

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  • AN IMPROVED METHOD FOR THE CONSTRUCTION OF HIGH-EFFICIENCY CDNA LIBRARY IN PLASMID OR LAMBDA VECTOR Reviewed

    K AGUAN, T KUSANO, N SUZUKI, Y KITAGAWA

    NUCLEIC ACIDS RESEARCH   18 ( 4 )   1071 - 1071   1990.2

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    DOI: 10.1093/nar/18.4.1071

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  • ISOLATION AND SEROLOGICAL COMPARISON OF VIRUS-CODED PROTEINS OF 3 POTYVIRUSES INFECTING CUCURBITACEOUS PLANTS Reviewed

    N SUZUKI, Y SHIRAKO, Y EHARA

    INTERVIROLOGY   31 ( 1 )   43 - 49   1990.1

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    DOI: 10.1159/000150133

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  • Sequence analysis of rice dwarf phytoreovirus genome segments S4, S5, and S6: Comparison with the equivalent wound tumor virus segments Reviewed

    Nobuhiro Suzuki, Yuzuko Watanabe, Tomonobu Kusano, Yoshichika Kitagawa

    Virology   179 ( 1 )   446 - 454   1990

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    DOI: 10.1016/0042-6822(90)90313-G

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  • Sequence analysis of the rice dwarf phytoreovirus segment s3 transcript encoding for the major structural core protein of 114 kDa Reviewed

    Nobuhiro Suzuki, Yuzuko Watanabe, Tomonobu Kusano, Yoshichika Kitagawa

    Virology   179 ( 1 )   455 - 459   1990

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    DOI: 10.1016/0042-6822(90)90314-H

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  • A simple method for elimination of non-specific reactions in non-precoated and electroblot enzyme-linked immunosorbent assay procedures used for detection of zucchini yellow mosaic virus Reviewed

    Suzuki, N, Shirako, Y, Ehara, Y

    Annals of the Phytopathological Society of Japan   56   337 - 341   1990

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  • NUCLEOTIDE-SEQUENCE OF RICE DWARF VIRUS SEGMENT-5

    N SUZUKI, Y WATANABE, T KUSANO, Y KITAGAWA

    NUCLEIC ACIDS RESEARCH   17 ( 21 )   8858 - 8859   1989.11

  • DISTRIBUTION OF CYLINDRICAL INCLUSION, AMORPHOUS INCLUSION AND CAPSID PROTEINS OF WATERMELON MOSAIC VIRUS-2 IN SYSTEMICALLY INFECTED PUMPKIN LEAVES Reviewed

    N SUZUKI, T KUDO, Y SHIRAKO, Y EHARA, T TACHIBANA

    JOURNAL OF GENERAL VIROLOGY   70   1085 - 1091   1989.5

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  • The use of SDS-polyacrylamide gel electrophoresis to assess purification procedure of three potyviruses infecting cucurbitaceous plants. Reviewed

    Suzuki, N, Shirako, Y, Ehara, Y

    Annals of the Phytopathological Society of Japan   55 ( 5 )   586 - 593   1989

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    We examined the effects of concentration methods, extraction buffers, clarification reagents, and resuspension buffers on virus yield and purity by using SDS-polyacrylamide gel electrophoresis (SDS-PAGE), so as to establish optimal purification procedures of zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus 2 (WMV2) and the watermelon mosaic virus 1 strain of papaya ringspot virus (PRSV-W). We then investigated the physicochemical properties of the three viruses. SDS-PAGE proved very useful to monitor virus purification in a small scale. The three viruses were purified with similar yields of 5-10 mg per 100 g of infected pumpkin leaves by the respective optimal methods. The procedures basically involved clarification with Triton X-100, differential centrifugation using a 20% sucrose pad, and 20-50% sucrose density gradient centrifugation. From WMV2 and PRSV-W purified preparations, capsid protein (CP) of 34,000 (34K) was predominantly detected in addition to minor degradation products of 27K to 33K, respectively. A breakdown product of 28K was, however, as detectabel as intact CP of 33K from ZYMV purified preparation. The RNAs of WMV2 and PRSV-W, which were a little larger than ZYMV RNA, were indistinguishable in size as evaluated with 1.7% agarose gel electrophoresis in denaturing conditions.

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    Other Link: http://agriknowledge.affrc.go.jp/RN/2010450878

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Books

  • 別種のウイルスから「殻」を借りる:「ヤドカリ」ウイルスのなぞ

    鈴木信弘( Role: Sole author ,  第17巻 第 3号 pp26-29)

    国立科学博物館 Milsil 「ミルシル」  2024.5 

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  • Structure of dsRNA Mycoviruses. Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Caston, J. R, Suzuki, N, Ghabrial, S. A

    Academic Press, Elsevier  2021.3 

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  • An introduction to fungal viruses. Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Suzuki, N.( Role: Sole author)

    2021.3 

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  • Viruses as Infectious Agents: Fungal, invertebrate, bacterial archaeal viruses Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman)

    Suzuki, N, Krell, P, Feiss, M, Prangishvili, M( Role: Joint author)

    Academic Press, Elsevier  2021.3 

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  • Yado-kari virus 1 and yado-nushi virus 1 (Unassigned). Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Das, S, Suzuki, N( Role: Joint author)

    Academic Press, Elsevier  2021.3 

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  • Megabirnaviruses (Megabirnaviridae). Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Sato, Y, Suzuki, N( Role: Joint author)

    Academic Press, Elsevier  2021.3 

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  • Quadriviruses (Quadriviridae). Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Kondo, H, Caston, J. R, Suzuki, N( Role: Joint author)

    Academic Press, Elsevier  2021.3 

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  • ネオウイルス学

    河岡, 義裕, 川口, 寧, 高橋, 英樹, 澤, 洋文, 朝長, 啓造, 松浦, 善治, 堀江, 真行, 佐藤, 佳, 鈴木, 信弘, 長崎, 慶三, 中川, 草, 野田, 岳志, 村田, 和義, 牧野, 晶子, 渡辺, 登喜子, 望月, 智弘, 大場, 靖子, 西浦, 博, 岩見, 真吾, 古瀬, 祐気( Role: Joint author ,  「ヤドカリ」「ヤドヌシ」と呼ばれるウイルスの共生関係)

    集英社  2021.3  ( ISBN:9784087211597

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    Total pages:315p   Language:Japanese

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  • 植物病理学第2版(眞山滋志・土佐幸雄編)

    鈴木信弘・大木理・上田一郎( Role: Contributor ,  ウイルス)

    文永堂  2020.3 

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  • Integration of viral sequences into eukaryotic host genomes: legacy of ancient infections

    ( Role: Joint editor)

    2019.3 

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  • Advances in Fungal Virus Research

    ( Role: Edit)

    2016 

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  • Viruses threatening stable production of cereal crops.

    ( Role: Joint editor)

    2015 

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  • The Springer index of viruses

    Tidona, Christian A, Darai, Gholamreza( Role: Contributor)

    Springer  2011  ( ISBN:9780387959184

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    Total pages:4 v.   Language:English

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  • Ninth Report of the International Committee for the Taxonomy of Viruses. A. M. Q. King et al., eds.

    Attoui, H., Mertens, P.P.C., Becnel, J., Belaganahalli, S., Bergoin, M., Brussaard, C.P., Chappell, J.D., Ciarlet, M., del Vas, M., Dermody, T.S., Dormitzer, P.R., Duncan, R., Fcang, Q., Graham, R., Guglielmi, K.M., Harding, R.M., Hillman, B., Makkay, A., Marzachì, C., Matthijnssens, J., Milne, R.G., Mohd Jaafar, F., Mori, H., Noordeloos, A.A., Omura, T., Patton, J.T., Rao, S., Maan, M., Stoltz, D., Suzuki, N., Upadhyaya, N.M., Wei, C. and Zhou, H.( Role: Contributor ,  Family Reoviridae.)

    Elsevier, Academic Press  2011 

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  • Encyclopedia of Virology 3rd Edition,

    Elsevier  2008 

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  • 作物保護の新展開ムバイオサイエンスのかけはしー

    ソフトサイエンス社  2005 

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  • Virus Taxonomy: Eighth Report of the International Committee for the Taxonomy of Viruses.

    Academic Press, San Diego.  2005 

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  • "Fungal dsRNA elements: concepts and application in agriculture, forestry and medicine."

    CRC Press. Boca Raton, Florida, USA.  2001 

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  • Virus Taxonomy: Seventh Report of the International Committee for the Taxonomy of Viruses.

    Academic Press, NY.  2000 

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MISC

  • Characterization of a closterovirus from wheat plants with yellowing leaf symptoms in Japan

    近藤秀樹, 兵頭究, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2024   2024

  • Replication of single partitiviruses in organisms across three kingdoms: Fungi, Plantae and Animalia

    TELENGECH Paul Kipkemboi, HYODO Kiwamu, ICHIKAWA Hiroaki, KONDO Hideki, SUZUKI Nobuhiro

    日本植物病理学会大会プログラム・講演要旨予稿集   2023   2023

  • Highjack of a host proviral vacuolar-type H+-ATPase by dianthoviruses

    兵頭究, 首藤丘宇, 近藤秀樹, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • The diversity of virus lifestyles in phytopathogenic filamentous fungi.

    佐藤有希代, 佐藤有希代, 近藤秀樹, 鈴木信弘

    日本植物病理学会植物感染生理談話会論文集   ( 56 )   2022

  • 「ヤドカリ」「ヤドヌシ」と呼ばれるウイルスの共生関係 Invited

    鈴木信弘

    ネオウイルス学(河岡義裕編)集英社   2021.3

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  • Virome analysis of aphid populations that infest the barley field

    近藤秀樹, 藤田美貴, 久野裕, 兵頭究, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021

  • Comparison of properties between two novel capsidless mycoviruses: a polymycovirus and a hadakavirus.

    佐藤有希代, SHAMSI W., SHAMSI W., JAMAL A., BHATTI M.F., 近藤秀樹, 鈴木信弘

    日本植物病理学会報   87 ( 1 )   2021

  • ウイルス

    鈴木信弘, 大木理, 上田一郎

    植物病理学第2版(眞山滋志・土佐幸雄編)文永堂   2020.3

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  • Inter-colony horizontal transmission of a capsidless 11-segmented RNA virus in Fusarium oxysporum: Are all segments transmitted?

    佐藤有希代, SHAMSI W., SHAMSI W., JAMAL A., BHATTI M. F., 近藤秀樹, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • 宿主足場タンパク質RACK1のハイジャックによる植物ウイルス増殖機構

    兵頭究, 鈴木信弘, 奥野哲郎

    日本植物病理学会報   85 ( 1 )   2019

  • 植物ウイルス感染の抗糸状菌植物免疫への影響

    兵頭究, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019

  • 宿主足場タンパク質RACK1は植物RNAウイルスの増殖を正に制御する

    兵頭究, 鈴木信弘, 奥野哲郎

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • Frontiers in fungal virology

    Nobuhiro Suzuki

    Journal of General Plant Pathology   83 ( 6 )   419 - 423   2017.11

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    DOI: 10.1007/s10327-017-0740-9

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  • 植物ラブドウイルスの分節化と進化

    近藤秀樹, 鈴木信弘

    日本進化学会大会プログラム・講演要旨集(Web)   19th   2017

  • 温度依存的に蓄積するGrifola frondosa RNA virus1(GfRV1)がマイタケ宿主遺伝子群の発現に及ぼす影響

    小松あき子, 近藤秀樹, 佐藤真之, 鈴木信弘, 藤森文啓

    日本微生物生態学会大会(Web)   2017   ROMBUNNO.O‐019 (WEB ONLY)   2017

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  • 食用キノコの子実体形成にウイルス感染が果たす役割についての研究(3)

    小松あき子, 佐藤真之, 佐藤真之, 佐藤真之, 近藤秀樹, 西堀耕三, 鈴木信弘, 藤森文啓, 藤森文啓

    東京家政大学生活科学研究所研究報告   39   57‐61 - 61   2016.7

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  • 活性酸素種は植物RNAウイルス増殖を正に制御する

    兵頭究, 鈴木信弘, 奥野哲郎

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • Advances in Fungal Virus Research Invited Reviewed

    SUZUKI Nobuhiro

    Virus Research   219   1 - 180   2016

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  • マイタケに感染するマイコウイルスの機能解析研究

    小松あき子, 佐藤真之, 近藤秀樹, 鈴木信弘, 藤森文啓

    日本分子生物学会年会プログラム・要旨集(Web)   39th   ROMBUNNO.1P‐0009 (WEB ONLY)   2016

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  • Functional Analysis of Viral Infection in the Fruit-Body Formation of Edible Mushroom(2)

    Komatsu Akiko, Sato Masayuki, Kondo Hideki, Sumi Mariko, Tsuchiya Yuki, Kurahashi Atsushi, Nishibori Kozo, Suzuki Nobuhiro, Fujimori Fumihiro

    東京家政大学生活科学研究所研究報告 = Bulletin of Research Institute of Domestic Science, Tokyo Kasei University   38   79 - 84   2015.7

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    東京家政大学生活科学研究所研究報告は、本研究所の本年度の活動成果を取りまとめたものです。本研究報告の内容の一部は、別途学会誌等に発表されることがありますのでご了承ください。

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  • 宿主ゲノム上に存在する RNA ウイルス感染記録を紐解く. Invited

    近藤秀樹, 千葉壮太郎, 鈴木信弘

    植物感染生理談話会論文集   50   133 - 142   2015

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  • Functional Analysis of Viral Infection in the Fruit-Body Formation of Edible Mushroom

    Komatsu Akiko, Kondo Hideki, Sato Masayuki, Tsuchiya Yuki, Kurahashi Atsushi, Nishibori Kozo, Suzuki Nobuhiro, Fujimori Fumihiro

    東京家政大学生活科学研究所研究報告 = Bulletin of Research Institute of Domestic Science, Tokyo Kasei University   37   109 - 114   2014.7

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    Language:Japanese   Publisher:東京家政大学生活科学研究所  

    東京家政大学生活科学研究所研究報告は、本研究所の本年度の活動成果を取りまとめたものです。本研究報告の内容の一部は、別途学会誌等に発表されることがありますのでご了承ください。

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  • Rosellinia necatrix fusarivirus 1:白紋羽病菌から分離された新規RNAウイルス

    ZHANG Rui, LIU Shengxue, 千葉壮太郎, 近藤秀樹, 兼松聡子, 鈴木信弘

    日本植物病理学会報   80 ( 1 )   2014

  • 植物および昆虫の核ゲノム上に見いだされたベニウイルス様配列

    近藤秀樹, 千葉壮太郎, 鈴木信弘

    日本植物病理学会報   80 ( 1 )   2014

  • マイタケに発見された新規RNAウイルスの性状と生物学的特性に関する研究

    角真理子, 佐藤真之, 小松あき子, 土屋(内田)有紀, 倉橋敦, 近藤秀樹, 鈴木信弘, 西堀耕三, 藤森文啓

    日本分子生物学会年会プログラム・要旨集(Web)   36th   2P-0014 (WEB ONLY)   2013

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  • Hypovirus Cysteine Proteases p29 and p48

    Nobuhiro Suzuki

    Handbook of Proteolytic Enzymes   2   2192 - 2195   2013

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    DOI: 10.1016/B978-0-12-382219-2.00492-0

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  • 白紋羽病菌とクリ胴枯病菌のマイコウイルス―病原力低下因子とヴァイロコントロール―農作物生産におけるウイルスの有効利用 Invited

    鈴木 信弘

    JATAFF ジャーナル   1   4 - 8   2013

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  • マイタケに存在する新規partitivirusの性状と生物学的特性に関する研究

    小松あき子, 佐藤真之, 佐藤真之, 佐藤真之, 角真理子, 土屋(内田)有紀, 倉橋敦, 近藤秀樹, 鈴木信弘, 西堀耕三, 藤森文啓, 藤森文啓

    日本分子生物学会年会プログラム・要旨集(Web)   36th   2013

  • Defective interfering(DI)RNAのRosellinia necatrix partitivirus2複製への影響

    LIU Yu-Hsin, 千葉壮太郎, 近藤秀樹, 兼松聡子, 鈴木信弘

    日本植物病理学会報   79 ( 1 )   2013

  • 植物ゲノムへ水平伝播された太古のパルティティウイルス配列

    千葉壮太郎, 近藤秀樹, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

    日本植物病理学会報   78 ( 1 )   2012

  • 植物の核ゲノム上に見いだされるマイナス鎖RNAウイルス様配列

    近藤秀樹, 千葉壮太郎, 梅林絵里, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2012   2012

  • Family Reoviridae. Invited

    Attoui, H, Mertens, P.P.C, Becnel, J, Belaganahalli, S, Bergoin, M, Brussaard, C.P, Chappell, J.D, Ciarlet, M, del Vas, M, Dermody, T.S, Dormitzer, P.R, Duncan, R, Fcang, Q, Graham, R, Guglielmi, K.M, Harding, R.M, Hillman, B, Makkay, A, Marzachì, C, Matthijnssens, J, Milne, R.G, Mohd Jaafar, F, Mori, H, Noordeloos, A.A, Omura, T, Patton, J.T, Rao, S, Maan, M, Stoltz, D, Suzuki, N, Upadhyaya, N.M, Wei, C, Zhou, H

    Virus Taxonomy: Ninth Report of the International Committee for the Taxonomy of Viruses.   2011

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  • 2La13 Establishment and application of a method for classification of Methylobacterium sp. by MALDI-TOF/MS

    Tani Akio, Okumura Marie, Kimbara Kazuhide, Suzuki Nobuhiro

    63   198 - 198   2011

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  • 非レトロRNAウイルス由来の植物染色体配列

    近藤秀樹, 千葉壮太郎, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2011   2011

  • Cymbidium chlorotic mosaic virusはソベモウイルス属の新規ウイルス種である

    近藤秀樹, 前田孚憲, 鈴木信弘

    日本植物病理学会報   77 ( 1 )   2011

  • 非パラレトロRNAウイルス由来の植物染色体遺伝子

    千葉壮太郎, 近藤秀樹, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

    日本ウイルス学会学術集会プログラム・抄録集   58th   2010

  • サギソウモザイクウイルスRNAの全塩基配列の決定

    丸山和之, 近藤秀樹, 前田孚憲, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2010   2010

  • Viral and host factors involved in symptom induction and replication of RNA viruses infecting phytopathogenic fungi.

    Salaipeth, L, Lin, Y.-H, Eusebio-Cope, A, Tanaka, T, Chiba, S, Suzuki, N

    Proceeding of the International Symposium on Fungal Genetics and Genomics   92 - 94   2009

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  • Potato virus X(PVX)サブゲノムRNAの蓄積はRNAサイレンシングにより根特異的に抑制される

    ANDIKA I. B., 近藤秀樹, 玉田哲男, 鈴木信弘

    日本植物病理学会報   75 ( 1 )   2009

  • (286) Soil-borne Virus-encoded Cysteine-rich Proteins Suppress RNA Silencing in Roots(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Andika I.B., Kondo H., Tamada T., Suzuki N.

    Annals of the Phytopathological Society of Japan   74 ( 3 )   243 - 243   2008.8

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  • (265) Induction of Mycoreovirus 1 Genome Rearrangements by the Multifunctional Protein p29 of the Prototype Hypovirus CHV1-EP713(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Suzuki N., Sun L.-Y.

    Annals of the Phytopathological Society of Japan   74 ( 3 )   237 - 237   2008.8

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  • (266) Assembly of Orchid Fleck Virus-like Particles in Nuclei is Associated with Formation of Viroplasm-like Structure(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Kondo H., Noda M., Tamada T., Suzuki N.

    Annals of the Phytopathological Society of Japan   74 ( 3 )   237 - 238   2008.8

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  • (32)Breakdown of RNA Silencing-mediated Resistance in BNYVV 54 kDa-RTD ORF Transgenic Plants by Internal Deletion of 54 kDa-RTD in the Viral Genome(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Andika I. B., Kondo H., Tamada T., Suzuki N.

    Annals of the Phytopathological Society of Japan   74 ( 1 )   64 - 64   2008.2

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  • (31)The Pathogenicity of M Structural Protein of Orchid Fleck Virus in Nicotiana benthamiana is Suppressed by the Interaction with Nucleocapsid Protein N(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Kondo H., Tamada T., Suzuki N.

    Annals of the Phytopathological Society of Japan   74 ( 1 )   64 - 64   2008.2

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  • Fungal viruses Invited

    Ghabrial, S, Suzuki, N

    Encyclopedia of Virology 3rd Edition   2   284 - 291   2008

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  • (229) Characterization of Strains of the Chestnut Blight Fungus, Cryphonectria Parasitica that are Silenced for Transgenes(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Maruyama K., Suzuki N.

    Annals of the Phytopathological Society of Japan   73 ( 3 )   236 - 236   2007.8

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  • (372) The Spread oi Rice dwarf virus among Cells of Its Insect Vector Exploits Virus-induced Tubular Structures(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Omura T., Wei Taiyun, Kikuchi A., Moriyasu Y., Suzuki N., Shimizu T., Hagiwara K., Chen Hongyan, Takahashi M., Ichiki-Uehara T.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   301   2006.11

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  • (370) Pns4 of Rice dwarf virus Is a Phosphoprotein, Is Localized around the Viroplasm Matrix, and Forms Minitubules(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Hagiwara K., Wei Taiyun, Kikuchi A., Suzuki N., Shimizu T., Chen Hongyan, Omura T.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   300 - 301   2006.11

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  • (341) Further Functional Analysis of the Papain-like Protease Encoded by Cryphonectria Hypovirus 1(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Sun L. Y., Maruyama K., Suzuki N.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   276 - 276   2005.8

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  • マイコウイルス学の新展開 Invited

    SUZUKI Nobuhiro

    144 - 157   2005

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  • Genus Mycoreovirus Invited

    Mertens, P. P. C, Wei, C, Suzuki, N, Hillman, B. I

    Virus Taxonomy: Eighth Report of the International Committee for the Taxonomy of Viruses.   556 - 560   2005

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  • Family Hypoviridae. Invited

    Nuss, D. L, Hillman, B. I, Rigling, D, Suzuki, N

    Virus Taxonomy: Eighth Report of the International Committee for the Taxonomy of Viruses.   597 - 601   2005

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  • Functinal Domains of Papain-like Protease p29 Encoded by the Prototype Hypovirus CHV1-EP713(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Suzuki N., Maruyama K.

    Annals of the Phytopathological Society of Japan   69 ( 3 )   315 - 316   2003.8

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  • ウイルスプロテアーゼ。

    鈴木信弘

    化学と生物 学会出版センター刊   41, 183-192. ( 5 )   335 - 344   2003

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    Language:Japanese   Publisher:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI: 10.1271/kagakutoseibutsu1962.41.335

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  • (210)Resistance to BNYVV in Transgenic Plants with 54 k Protein Coding Domain Is Mediated by the RNA Silencing Mechanism

    Andika I.B., Kondo H., Suzuki N., Tamada T.

    Annals of the Phytopathological Society of Japan   68 ( 2 )   212 - 212   2002.8

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  • ウイルスをもってカビを制す

    鈴木 信弘

    化学と生物 (日本農芸化学会編)   36   524 - 526   1998

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  • (311) Phosphorylation and Intracellular Localization of a Rice Dwarf Phytoreovirus Nonstructural Protein, Pns12(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    SUZUKI N., HOSOKAWA D., MATSUURA Y., SHIRAKO Y., KIKUCHI A., OMURA T.

    Annals of the Phytopathological Society of Japan   63 ( 3 )   268 - 268   1997.6

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  • (312) Immunogold Localization of Rice Dwarf Virus Proteins, Pns4 and Pns10, Expressed by Recombinant Baculoviruses in Insect Cells(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    HOSOKAWA D., SUZUKI N.

    Annals of the Phytopathological Society of Japan   63 ( 3 )   268 - 269   1997.6

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  • Structure/function analysis of rice dwarf phytoreovirus genome and its proteins.

    Suzuki Nobuhiro

    Uirusu   47 ( 2 )   145 - 154   1997

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    Language:Japanese   Publisher:The Japanese Society for Virology  

    DOI: 10.2222/jsv.47.145

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  • Purification of Rice Dwarf Phytoreoviral Proteins Highly Expressed in Spodoptera frugiperda Cells

    SUZUKI N., SUGAWARA M.

    Annals of the Phytopathological Society of Japan   62 ( 6 )   600 - 600   1996.12

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  • Immunogold Localization of Rice Dwarf Phytoreovirus-coded Nonstructural Proteins in Infected Barley Cells

    HOSOKAWA D., SUZUKI N.

    Annals of the Phytopathological Society of Japan   62 ( 3 )   339 - 339   1996.6

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  • Polycistronic Phytoreovirus Genome Segment

    SUZUKI N., SUGAWARA M., NUSS D.L., MATSUURA Y.

    Annals of the Phytopathological Society of Japan   62 ( 3 )   339 - 339   1996.6

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  • Detection of Rice Dwarf Phytoreovirus RNA by RT-PCR

    KOBAYASHI J., KONDOH N., KATSUMOTO K., SUZUKI N., MIYAJIMA S.

    Annals of the Phytopathological Society of Japan   61 ( 3 )   281 - 281   1995.6

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  • Characterization of Rice Dwarf Phytoreovirus P5

    SUZUKI N., SUGAWARA M., KUSANO T., MATSUURA Y., OMURA T.

    Annals of the Phytopathological Society of Japan   61 ( 3 )   281 - 281   1995.6

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  • A Structural Protein Encoded by Rice Dwarf Phytoreovirus Segment S5

    SUZUKI N, SUGAWARA M

    Annals of the Phytopathological Society of Japan   60 ( 6 )   763 - 763   1994.12

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  • Quantitative Detection of Rice Dwarf Phytoreovirus-Coded Proteins in Both Plant and Insect Hosts

    SUZUKI N., MORI H., SUGAWARA M., KUSANO T., MATSUURA Y.

    Annals of the Phytopathological Society of Japan   60 ( 3 )   382 - 383   1994.6

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  • Immunodetection of Rice Dwarf Phytoreovirus Nonstructural Proteins in Both Infected Rice and Leafhopper

    SUZUKI N., SUGAWARA M., KUSANO T., MATSUURA Y.

    Annals of the Phytopathological Society of Japan   59 ( 6 )   763 - 764   1993.12

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  • Rice Dwarf Phytoreovirus S12 Transcript is Tricistronic in vitro

    SUZUKI N., SUGAWARA M., KUSANO T.

    Annals of the Phytopathological Society of Japan   59 ( 3 )   342 - 343   1993.6

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  • Detection of Rice Dwarf Phytoreovirus-Coded Proteins in Viruliferous Leafhoppers

    SUZUKI N., MORI H., SUGAWARA M.

    Annals of the Phytopathological Society of Japan   59 ( 3 )   328 - 329   1993.6

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  • Molecular Analysis of Rice Dwarf Phytoreovirus Segment S1 : Interviral Homo1ogy of the Putative RNA-Dependent RNA Polymerase between Plant- and Animal-Infecting Reoviruses

    SUZUKI S., TANIMURA M., WATANABE Y., KUSANO T., KITAGAWA Y., SUDA N., KUDO H., UYEDA I., SHIKATA E.

    Annals of the Phytopathological Society of Japan   58 ( 4 )   630 - 631   1992.10

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  • Reactivities of Monoclonal Antibodies to Cylindrical Inclusion, Amorphous Inclusion, and Capsid Proteins of Watermelon Mosaic Virus 2 with Corresponding Proteins of Other Potyviruses

    SUZUKI Nobuhiro, SHIRAKO Yukio, EHARA Yoshio

    Annals of the Phytopathological Society of Japan   57 ( 5 )   706 - 710   1991.12

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Presentations

  • From neovirology to phytopathology.

    Suzuki, N

    The first Chinese Mycovirus Symposium  2023.11.25 

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    Event date: 2023.11.24 - 2023.11.27

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  • Yadokari/yadonushi nature: A virus in a virus in a fungus in a plant. Invited

    Sato, Y, Suzuki, N

    The Second International Molecular Plant Protection Congress. Turkey.  2023.5.15 

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    Event date: 2023.5.14 - 2023.5.21

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  • Experimental and practical control measures for white root rot in fruit trees in Japan. Invited

    Suzuki, N

    Yangling International Agri-science Forum. 

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    Event date: 2021.10.23 - 2021.10.24

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  • Partnership between capsidless (+)ssRNA yadokariviruses and their host dsRNA viruses: Promiscuous or virtuous? Invited

    Suzuki, N

    Awaji International Forum on Immunity and Infection. 

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    Event date: 2021.9.29 - 2021.10.1

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  • A neo-virus-lifestyle exhibited by a (+)RNA virus hosted by an unrelated dsRNA virus in a phytopathogenic fungus. Invited

    Suzuki, N

    The Spring Meeting of the Korean Society of Plant Pathology (KSPP 2018)  2018.4.26 

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    Event date: 2018.4.26 - 2018.4.27

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  • Fungal RNA silencing involved in antiviral defense and RNA recombination. Invited

    Suzuki, N

    ASV 2016 Annual Meeting  2016.6.18 

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    Event date: 2016.6.18 - 2016.6.22

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  • Antiviral defense in the phytopathogenic filamentous ascomycete, Cryphonectria parasitica. Invited

    Suzuki, N

    Swiss Society for Microbiology Annual Assembly and Meeting  2016.6.14 

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    Event date: 2016.6.13 - 2016.6.16

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  • Another nude virus: a capsidless ssRNA virus hosted by an unrelated dsRNA virus. Invited

    Suzuki, N

    International Symposium on Virology and Fungal Genetics  2016.1.15 

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    Event date: 2016.1.15

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  • Highly activated RNA silencing via strong induction of dicer by one virus can interfere with the replication of an unrelated virus. Invited

    Chiba, S, Suzuki, N

    BMB2015 Biochemistry and Molecular Biology  2015.12.2 

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    Event date: 2015.12.1 - 2015.12.4

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  • A new virus lifestyle challenging the virus rules and concepts. Invited

    A new virus, lifestyle challenging the virus rules, concepts

    JICA Africa-ai-Japan Project/JSPS-AASP Sponsored Workshop on Plant Science and Resource Innovative Research Core with Pan African University.  2015.10.19 

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    Event date: 2015.10.19

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  • From virology to plant disease control. Invited

    Suzuki, N

    JSPS Core-to-Core Program Sponsored Workshop on Crop Science and Innovation for Agriculture.  2015.10.15 

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    Event date: 2015.10.15

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  • A new virus life style challenging the virus rules and concepts. Invited

    Zhang, R, Hisano, S, Tani, A, Kondo, H, Kanematsu, S, Suzuki, N

    13th Spanish Virology Congress  2015.6.8 

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    Event date: 2015.6.7 - 2015.6.10

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  • Virus interference in plants and fungal hosts. Invited

    Suzuki N

    9th JKUAT Scientific and Technological Conference 2014  2014.11.13 

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    Event date: 2014.11.13 - 2014.11.14

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  • The chestnut blight fungus for studies on virus/host and virus/virus interactions: from a natural to a model host. Invited

    Suzuki N

    EMBO Conference Viruses of microbes: Structure and function, from molecules to communities.  2014.7.16 

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    Event date: 2014.7.14 - 2014.7.18

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  • Dual functionality of fungal Dicer in multilayer antiviral defense. Invited International conference

    SUZUKI Nobuhiro

    International Symposium on Virus Diseases of Important Crops.  2019.9 

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  • Dicer functions transcriptionally and post-transcriptionally in a multilayer antiviral defense Invited International conference

    Suzuki, N, Andika, I. B, Kondo, H

    European Congress of Virology 2019  2019.5 

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  • Another nude virus: a capsidless ssRNA virus hosted by an unrelated dsRNA virus. Invited International conference

    SUZUKI Nobuhiro

    13th International dsRNA Virus Symposium  2018.9 

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  • Viruses as biological control (virocontrol) agents of plant fungal pathogens. Invited

    Suzuki, N., and Kanematsu, S.

    The First International Congress of Biological Control.  2018.5 

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  • A neo-virus-lifestyle exhibited by a (+)RNA virus hosted in an unrelated dsRNA virus Invited

    N. Suzuki.

    The 16th Awaji International Forum on Infection and Immunity.  2017.9 

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  • Mutualist Interactions between a Capsidless (+) ssRNA Virus and a dsRNA Virus Occurring in a Phytopathogenic Fungus. Invited

    Suzuki, N.

    ASM Microbe 2017  2017.6 

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  • Virocontrol measures for phylopathogenic fungi. Invited

    Suzuki, N

    Special seminar series for phytopathology.  2023.11.23 

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    Event date: 2023.11.23

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  • Possible yadonusi/yadokari nature observed in a Japanese strain NBRC 4031 of Aspergillus foetidus.

    Fadli, M, Hisano, S, Kondo, H, Suzuki, N

    The 42nd Annual Meeting of the American Society for Virology 2023  2023.6.26 

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    Event date: 2023.6.24 - 2023.6.28

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  • Autophagy promotes the replication of red clover necrotic mosaic virus.

    Hyodo, K, Sudo, K, Kondo, H, Suzuki, N

    The 42nd Annual Meeting of the American Society for Virology 2020. June 24-28, 2023.  2023.6.25 

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    Event date: 2023.6.24 - 2023.6.28

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  • Replication of single partitiviruses in hosts across three kingdoms; Fungi, Plantae and Animalia

    Telengech, P, Hyodo, K, Ichikawa, H, Kondo, H, Suzuki, N

    The 42nd Annual Meeting of the American Society for Virology 2023  2023.6.25 

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    Event date: 2023.6.24 - 2023.6.28

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  • Viruses in the causal agents of dry bubble disease: Novel candidates for biological candidates for biological pest management.

    Lóránt Hatvani, Hideki Kondo, Nobuhiro Suzuki, Sándor Kocsubé, Helen Grogan

    Mushroom Days Brabanthallen 's-Hertogenbosch  2023.5.11 

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    Event date: 2023.5.10 - 2023.5.12

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  • Structure and assembly of fungal dsRNA viruses

    Gil-Cantero1, D, Novoa, G, Martínez-Romero, J. M, Luque, D, Nobuhiro Suzuki, N, Castón, J. R

    8th European Congress of Virology.  2023.5.5 

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    Event date: 2023.5.4 - 2023.5.7

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  • Mycoviruses: A novel approach for controlling mushroom pathogenic fungi.

    Hatvani, L, Kondo, H, Shahi, S, Suzuki, N, Grogan, H

    Power of Fungi and Mycotoxins in Climate Change  2022.9.16 

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    Event date: 2022.9.16 - 2022.9.17

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  • A new tetra-segmented splipalmivirus with divided RdRP domains from Cryphonectria naterciae, a fungus found on chestnut and cork oak trees in Europe

    Paul Telengech, Yukiyo Sato, Sabitree Shahi, Sakae Hisano, Carolina Cornejo, Daniel Rigling, Hideki Kondo, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium  2022.6.1 

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    Event date: 2022.5.30 - 2022.6.2

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  • A transfectable fusagravirus from Cryphonectria carpinicola with spherical particles

    Sakae Hisano, Subha Das, Hideki Kondo, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium.  2022.6.1 

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    Event date: 2022.5.30 - 2022.6.2

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  • Fusagraviruses in Cryphonectria species

    Carolina Cornejo, Sandra Gaehwiler, Wajeeha Shamsi, Hideki Kondo, Nobuhiro Suzuki, Daniel Rigling

    The fifth International Mycovirus Symposium  2022.6.1 

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    Event date: 2022.5.30 - 2022.6.2

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  • Synonymous codon usage bias in Cryphonectria parasitica viruses: Insights on evolution and host adaptation Invited

    Zeeshan Zahid, Alanna B. Cohen, Nobuhiro Suzuki, Bradley I. Hillman

    The fifth International Mycovirus Symposium  2022.6.1 

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    Event date: 2022.5.30 - 2022.6.2

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  • Partnership between capsidless (+)ssRNA yadokariviruses and their host dsRNA viruses: Promiscuous or virtuous? Invited

    Yukiyo Sato, Sakae Hisano, Carlos Lopez Hererra, Hideki Kondo, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium  2022.5.31 

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    Event date: 2022.5.30 - 2022.6.2

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  • Low-level IRES activity likely contributes to the replication restriction of Cryphonectria hypovirus 1 in Cryphonectria carpinicola Invited

    Sabitree Shahi, Sotaro Chiba, Yoshihiro Takaki, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium  2022.5.31 

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    Event date: 2022.5.30 - 2022.6.2

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  • Argonaute-independent antiviral RNA interference in the chestnut blight fungus, Cryphonectria parasitica.

    Yukiyo Sato, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium. Gargnano,  2022.5.31 

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    Event date: 2022.5.30 - 2022.6.2

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  • Diverse partitiviruses from the phytopathogenic fungus, Rosellinia necatrix.

    Telengech, P, Hisano, S, Micheni, C. M, Hyodo, K, Arjona-Lopez, J.M, Lopez-Herrera, C, Kanematsu, S, Kondo, H, Suzuki, N

    The 40th Annual Meeting of the American Society for Virology 2020. 

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    Event date: 2021.7.19 - 2021.7.23

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  • Partnership between capsidless (+)ssRNA yadokariviruses and their host dsRNA viruses: Promiscuous or virtuous?

    Yukiyo Sato, Sakae Hisano, Subha Das, Carlos Lopez Hererra, Hideki Kondo, Nobuhiro Suzuki

    The 40th Annual Meeting of the American Society for Virology 2020. 

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    Event date: 2021.7.19 - 2021.7.23

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  • Prevalence, diversity and impact of mycoviruses infecting plant pathogenic fungi pf rice in the Philippines.

    Guinto, T, Cope, A, Urzo, M, Budot, B, Yanoria, M. J, Jonson, G, Oliva, R, Kondo, H, Suzuki, N

    Philippine Phytopathological Society Pest Management Council of the Philippines, Inc. PMCP 2021 

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    Event date: 2021.7.6 - 2021.7.7

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  • In-tree behavior of diverse viruses infecting the chestnut blight fungus.

    Suzuki, N, Aulia, A, Shahi, S, Hillman, B. I, Cornerjo, C, Rigling, D

    The 39th Annual Meeting of the American Society for Virology 2020. 

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    Event date: 2020.6.13 - 2020.6.17

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  • 2A-mediated co-translational cleavage is prerequisite for the viability of yado-kari virus 1 hosted by yado-nushi virus 1.

    Das, S, Alam, M, Zhang, R, Hisano, S, Sato, Y, Kondo, H, Suzuki, N

    The 39th Annual Meeting of the American Society for Virology 2020 

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    Event date: 2020.6.13 - 2020.6.17

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  • Maintenance and loss of dispensable genomic segments of a polymyco-like capsidless fungal virus with an eleven-segmented RNA genome during its infection cycle.

    Sato, Y, Shamsi, W, Jamal3, A, Bhatti, M. F, Kondo, H, Suzuki, N

    The 39th Annual Meeting of the American Society for Virology 2020 

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    Event date: 2020.6.13 - 2020.6.17

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  • Vigorous induction of dicer-like 2 gene by a virus interferes with an unrelated virus in the model phytopathogenic fungus Cryphonectria parasitica. Invited

    Chiba, S, Suzuki, N

    12th PSJ Plant Virus Disease Workshop:The Interface between plant and fungal viruses II.  2016.3.14 

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    Event date: 2016.3.14

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  • A new virus life style challenging the virus rules and concepts. Invited

    R. Zhang, S. Hisano, A. Tani, H. Kondo, S. Kanematsu, N. Suzuki

    63rd Annual Meeting of the Japanese Society for Virology  2015.11.23 

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    Event date: 2015.11.22 - 2015.11.24

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  • The World of Fungal Viruses Invited

    Nobuhiro SUZUKI

    Rob Goldbach Lecture Event at Wageningen University  2024.10.31 

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  • Mycoviruses in C. parasitica and their biological control potential. Invited International conference

    SUZUKI Nobuhiro

    WSL Colloquium.  2019.10.30 

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Awards

  • Editor of Distinction Awards 2025

    2025.5   Springer Nature  

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  • Rob Goldbach Lecuture Award

    2024.10   Wageningen University  

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  • Sanyo-Shimbun Scientific Achievement Award

    2024.1   Sanyo-Shimbun  

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  • Top Peer Reviewer Award

    2019.9   Publon (Web of Science Group)  

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  • 学会賞

    2017.3   日本植物病理学会  

    鈴木 信弘

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  • 日本ウイルス学会奨励賞

    1997  

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    Country:Japan

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  • 日本植物病理学会奨励賞

    1995  

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    Country:Japan

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Research Projects

  • Investigation into the potential role of fungal viruses in the dry bubble disease of cultivated mushroom – towards biological pest management

    Grant number:Leca-VIR  2022.04 - 2023.10

    European Commission (EU) Research Leaders 2025/Marie Skłodowska Curie Fellowship (2022-2025) 

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid) 

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  • Frontiers in myco-immunity research and new development in relevant phytopathology

    Grant number:21H05035  2021.07 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

    鈴木 信弘

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    Grant amount:\188240000 ( Direct expense: \144800000 、 Indirect expense:\43440000 )

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  • Virus infection across the three kingdoms Plantae, Fungi, and Animalia: defense/counter-defense and host adaptation

    Grant number:21K18222  2021.07 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Pioneering)

    鈴木 信弘

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    Grant amount:\25870000 ( Direct expense: \19900000 、 Indirect expense:\5970000 )

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  • Neo-lifestyle of fungal viruses

    Grant number:16H06436  2016.06 - 2021.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Suzuki Nobuhiro

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    Grant amount:\117520000 ( Direct expense: \90400000 、 Indirect expense:\27120000 )

    The objective of this project is to establish two neo-lifestyles of fungal viruses and explore their generality and ecological significance. First, we have proved the yadokari/ yadonushi nature in Japanese strains of Rosellinia necatrix and revealed its molecular basis. Capsidless yadokari virus 1 (YkV1) highjacks the capsid of yadnushi virus 1 (YnV1) to replicate in the heterocapsid using its own RNA-dependent RNA polymerase, while in return YkV1 trans-enhances the replication of YnV1. For the hadaka nature, we have shown that hadaka virus 1 (HadV1) from a Pakistani strain of Fusarium oxysporum exists as a capsidless naked form at least in mycelial homogenates that is accessible by RNase A and unable to be pelleted by untracentrifugation. While the yadonushi/yadokari nature has been observed not only foreign fungal strains but also in plants,the hadaka nature has also been detected in other filamentous fungi, suggesting suggesting the generality of the two viral neo-lifestyles.

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  • Antiviral innate immunity in phytopathogenic fungi

    Grant number:25252011  2013.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    SUZUKI Nobuhiro

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    Grant amount:\45760000 ( Direct expense: \35200000 、 Indirect expense:\10560000 )

    The objective of this project is to explore RNA silencing (RNAi) as the antiviral innate defense against fungal viruses in plant pathogenic filamentous fungi. Consequently, a great progress has been made, leading to breakthroughs: 1) Discovery of RNAi mediated antiviral defense that requires Dicer but not AGO in Cryphonectria parasitica, 2) Detection of inhibitory effects on RNA silencing activities against RNA and DNA viruses, and transgenes in Pyricularia oryzae, 3) Development of a screening protocol for host factors required for virus perception through transcription induction of key genes of RNAi upon virus infection in C. parasitica, 4) Identification of the SAGA complex as a transcriptional regulator of fungal RNA silencing. These findings will bring about a paradigm shift in RNAi. Fungi are now established as the third position next to animals and plants in antiviral defense research. Note that most of the obtained data were published in international, high-quality journals.

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  • Platform construction for research into viruses infecting phytopathogenic obligate fungi and challenge for their biological control

    Grant number:23K18029  2023.06 - 2026.03

    JSPS (Japan Society for the Promotion of Science  科学研究費助成事業  挑戦的研究(萌芽)

    近藤 秀樹, 鈴木 信弘

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    Grant amount:\6500000 ( Direct expense: \5000000 、 Indirect expense:\1500000 )

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  • Studies on the rhizospheric virome of crop holobiants and its ecological roles

    Grant number:23H02214  2023.04 - 2027.03

    JSPS (Japan Society for the Promotion of Science  科学研究費助成事業  基盤研究(B)

    近藤 秀樹, 久野 裕, 兵頭 究, 鈴木 信弘

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    Authorship:Coinvestigator(s) 

    Grant amount:\18590000 ( Direct expense: \14300000 、 Indirect expense:\4290000 )

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  • 作物超個体における根圏RNAウイルス叢の実体解明とその生態学的役割

    Grant number:23K26907  2023.04 - 2027.03

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    近藤 秀樹, 久野 裕, 兵頭 究, 鈴木 信弘

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    Grant amount:\18590000 ( Direct expense: \14300000 、 Indirect expense:\4290000 )

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  • Infection across three kingdoms by single viruses

    Grant number:22F22095  2022.07 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows  Grant-in-Aid for JSPS Fellows

    鈴木 信弘, TELENGECH PAUL

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    Grant amount:\2300000 ( Direct expense: \2300000 )

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  • In-tree behavior of diverse viruses harbored in the chestnut blight fungus Cryphonectria parasitica and their biocontrol potential

    Grant number:JPJSBP120  2022.04 - 2024.03

    JSPS  二国間交流事業 オープンパートナーシップ共同研究 

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    Authorship:Principal investigator 

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  • モデル糸状菌アカパンカビを用いたウイルス研究フロンティア

    Grant number:21K19086  2021.07 - 2023.03

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

    近藤 秀樹, 鈴木 信弘, 本田 信治

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    Grant amount:\6500000 ( Direct expense: \5000000 、 Indirect expense:\1500000 )

    本課題では、菌類ウイルス学のモデル実験系を構築するため、代表的な実験系糸状菌の一種であるアカパンカビを用いてウイルス学を展開するための基盤整備を進めている。以下に本年度の研究実績を示す。
    柱① アカパンカビの感染性ウイルスを用いたウイルス学:先の報告で見出されたアカパンカビに自然感染する新規ウイルスの性状解析を進を進めている(Hondaら, Nature Comm 2020、未発表)。これまでに、Neurospora crassaのNGSデータでデルタフレキシ及び新奇RNAウイルス(配列断片)が見出された菌株をついて、感染ウイルスのゲノム配列解析を進めている。さらに、Neurospora intermediaのNGS解析で見出されたウイルス様コンテイグ(フザリウイルス、ミトウイルス、デルタフレキシウイルス)について、元菌株のRT-PCRによりその存在確認と部分配列の決定をおこなった。一部ウイルスについては、3'RLM-RACEによる末端配列を進めている。これらのウイルスのRdRP配列に基づく分子系統樹(最尤法)を作成し、系統学的位置付けを検討した。
    柱② ウイルス病徴発現・複製、防御機構/ウイルス反撃に関与する因子の同定と機構解明:N. crassaの抗ウイルスRNAi機構の鍵酵素であるdcl1, dcl2 qde2の単独欠損変異株(dcl1、dcl2, qde2)、二重欠損変異株(dcl1/dcl2, qde2/dcl1, qde2/dcl2)、三重欠損変異体(qde2/dcl1/dcl2)および野生型を用いて、異なるゲノムタイプの二種マイコウイルス(一本鎖RNAウイルスと分節型dsRNAウイルス)の混合感染株をそれぞれ作出した。これらの混合感染株について全RNAを抽出し、小分子RNA分画のRNA-seq解析を行った。現在,得られたRNA-seqの配列データの詳細解析を進めている。

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  • Frontier in mycoimmunity research and its new developments in phytopathology

    Grant number:21H04727  2021.04 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    鈴木 信弘, 近藤 秀樹, 兵頭 究

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    Grant amount:\41600000 ( Direct expense: \32000000 、 Indirect expense:\9600000 )

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  • 宿主アカパンカビの抗ウイルス免疫暴走化機序の解明

    Grant number:21H02153  2021.04 - 2024.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    本田 信治, 鈴木 信弘

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    Grant amount:\17420000 ( Direct expense: \13400000 、 Indirect expense:\4020000 )

    本研究代表らは構築したモデル糸状菌アカパンカビのウイルス感染系(Honda et al, Nature Comm, 2020)を用いて、宿主がウイルス感染に対して過剰に免疫応答することで、病気になることを発見した。本研究の目的は、この免疫暴走化に関わる「ウイルス認識機構とその下流因子」、免疫暴走の鍵となる「病的原因遺伝子」を同定し、その分子機序を解明することである。
    これまでの解析により、アカパンカビのDICERであるDCL-2を欠損させると、免疫暴走化が抑制されて病気から回復することを見出している。この結果から、DCL-2が有するdsRNA結合部位がウイルスのゲノムdsRNAを認識し、下流の免疫暴走の鍵となる「病的原因遺伝子」の転写を活性化すると考えた。そこで、研究計画に沿って種々の機能変異DCL-2を有する形質変換株を作製し、解析を行った。その結果、DCL-2のdsRNA結合部位、及び、DICER活性が免疫暴走化に関与することを確認した。
    また、クリ胴枯病菌ではSAGA複合体がウイルス応答遺伝子群の転写活性化に関与していることが報告されていることから(Andika et al, PNAS, 2017)、SAGA複合体を介する転写活性化と免疫暴走化の関係を調査した。その結果、DCL-2欠損と同様に、SAGA複合体の構成因子を欠損させると、免疫暴走化が抑制されることから、アカパンカビとクリ胴枯病菌に共通するウイルス応答機構の存在が示唆された。

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  • Host range determinants of a virocontrol agent for the chestnut blight disease

    Grant number:21F21093  2021.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows  Grant-in-Aid for JSPS Fellows

    鈴木 信弘, SHAHI SABITREE

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    Grant amount:\2300000 ( Direct expense: \2300000 )

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  • ネオウイルス学:生命の源流から超個体、そしてエコ・スフィアーへ

    Grant number:21H00456  2021.04 - 2022.03

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    河岡 義裕, 朝長 啓造, 澤 洋文, 松浦 善治, 川口 寧, 渡辺 登喜子, 鈴木 信弘, 高橋 英樹, 長崎 慶三

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    Grant amount:\3900000 ( Direct expense: \3000000 、 Indirect expense:\900000 )

    「ネオウイルス学」領域内の計画研究および公募研究で得られた各班それぞれの研究テーマについて研究成果を取りまとめ、さらに班間、あるいは、「共進化」、「共生」、「多様性」ユニット間の連携テーマの重要性・意義を考慮しつつ、領域全体の研究成果を取りまとめた。業績集を作成し、冊子体として印刷して領域内外で配布することによって、本領域の研究成果を発信した。報告内容は、出来るだけ分かりやすい記述を心がけ、学術分野だけでなく、一般国民にも読んでもらえるような業績集を作成した。また、高校生、大学生、一般の国民に向けた講演会やセミナー、そして領域ホームページとTwitterによって、領域の活動内容や研究成果を報告することによって、国民に向けた研究成果の発信に努めた。また、新しい試みとして「ウイルスおりがみ」を作成した。ウイルスおりがみは、おりがみを折ることで、一般国民にウイルスの面白さを知ってもらうことを目的とした、科学コミュニケーションツールであり、公募研究班の牧野晶子助教(京都大)、デザイン・ユニットCOCHAE、合同会社SHIBUYA PUBLISHING & BOOKSELLERS、三宅文子アート・プランナーらのグループによって作成された。ウイルスおりがみには領域内の研究者による解説も加えられており、ウイルスの基本、構造の面白さ、役に立つウイルス、生き物の中にいる多様なウイルスについて学ぶことができる。ウイルスおりがみの配布の公募をしたところ2288部の応募があり、抽選で500部を無償で配布した。日本デザイン振興会が主催するグッドデザイン賞へウイルスおりがみを出品中である。

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  • 作物根圏におけるウイルス叢の多様性とその感染動態から紐解く生態的意義

    Grant number:20H02987  2020.04 - 2023.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    近藤 秀樹, 久野 裕, 鈴木 信弘, 兵頭 究

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    Grant amount:\17680000 ( Direct expense: \13600000 、 Indirect expense:\4080000 )

    本課題では、ムギ類生存圏(特に根系)におけるウイルス叢の多様性・普遍性や動態を紐解き、さらにそれらの未知なる生態学的役割の一端に迫ることを目指している。以下に本年度の研究実績を示す。
    柱①根圏に存在する主要な菌類・昆虫ウイルス群の宿主探索:初年度に引き続き、ムギ類(オオムギ・コムギ)の地上部・根系、さらにアブラムシ、うどんこ病菌などのRNAseqデータを取得した。得られたデータセットはウイルス配列の確認作業や分子系統解析を進めている。さらに、北海道のコムギサンプルより見出された新規フレキシウイルス(WVQ)の解析の結果,当該圃場に三系統が存在し、ライムギにも感染できること、さらに土壌伝染性ウイルスの可能性が高いことを見出している。根系ウイルス叢のリザーバー探索については、本年度はオオムギ根のRNAseqデータに含まれる微生物リードの抽出と配列相同性解析により、微生物叢の概要を調査した。
    柱②根圏ウイルスの生物界を跨ぐ水平伝搬ポテンシャルと宿主への影響:昨年度より継続中のルテオウイルスなど新規植物ウイルスのゲノム解析を進めており、RT-PCRおよびRLM-RACE解析により全長ゲノムRNA配列を確認・決定した。また、圃場のうどんこ病菌のウイルスについては、今年度はdsRNAを鋳型にした全長cDNAのPCR増幅法の検討を進め、そのアンプリコン配列解析により未同定のゲノム末端配列の解析を進めている。
    柱③根圏ウイルス叢の年次変動とムギ類ウイルスの病原性の(再)評価:核酸抽出を伴わない簡易ウイルス診断法(RT-PCRをベースとする)の条件を設定し、一部圃場サンプル(葉)でルテオウイルス等の検出を進めている。現在、圃場オオムギサンプル(保存している過年度のを含む)について網羅的に検定すすめている。また、アブラムシ媒介性新規植物ウイルスについては、チャンバー内試験にて病原性評価を進めている。

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  • ヤドカリウイルスの新奇生活スタイル(宿借性)の証明

    Grant number:18F18086  2018.04 - 2020.03

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, DAS SUBHA

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    Grant amount:\2300000 ( Direct expense: \2300000 )

    果樹白紋羽病菌のウイルスハンティングの過程で、ユニークなウイルス/ウイルス間相互作用を発見した。本菌から得られるウイルス精製画分からは、ヤドヌシウイルス(YnV1), ヤドカリウイルス(YkV1)が検出された。YnV1は非分節型dsRNAウイルスで、 キャプシド蛋白質(CP)とRNA合 成酵素(RdRp)をコードする。YkV1は RdRpドメインとプロテアーゼドメインをコードするプラス(+ )1本鎖RNAウイルスである。
    具体的に下記の項目を実施し、「ヤドカリウイルスYkV1の宿借性]のコンセプトの確立に努めた。
    1) 昨年作成したYkV1 RdRp GDDモティーフの変異株をさらに増やし、同様の解析を進め、「宿借性」にYkV1 RdRpが必須であることを確認した。
    2) 昨年合成した6つの2A様プロテアーゼ変異株に加え、さらに6つの変異をYkV1感染性cDNA上で変異を導入し、感染性の有無とプロテアーゼ活性を調べた。その結果、バキュロウイルスを用いた昆虫細胞内でのプロテアーゼ活性と宿主菌でのウイルス複製能が一致した。すなわち、プロテアーゼ活性を消失した変異株は、白紋羽病菌での複製能も消失した。興味深いことに、弱いプロテアーゼ活性を有する変異株は、長期間維持すると2A様プロテアーゼが野生型に復帰したウイルス株が出現した。以上の結果は、YkV1の複製には、RdRp領域がポリプロテインから切断される必要があることを強く示唆する。
    3) YkV1/YnV1感染株から塩化セシウム平衡密度勾配遠心法により、粒子を分画した結果、それぞれ単独で粒子化されていることを示唆するデータを得た。

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  • Frontier of research on antiviral immunity in phytopathogenic filamentous fugi

    Grant number:17H01463  2017.04 - 2021.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    Suzuki Nobuhiro

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    Grant amount:\41990000 ( Direct expense: \32300000 、 Indirect expense:\9690000 )

    The objective of this project is to explore RNA silencing (RNAi) against fungal viruses in plant pathogenic filamentous fungi. A great progress has been made, leading to several breakthroughs: 1) Discovery of RNAi mediated antiviral defense that requires Dicer but not AGO in the chestnut blight fungus, 2) Identification of the SAGA complex as a transcriptional regulator of fungal RNA silencing, 3) Discovery of a novel antiviral defense mechanism by which virus-induced symptom expression is alleviated by transcriptional upregulation of many host genes upon virus infection in C. parasitica, and 4) Identification of Dicer and SAGA (universal transcriptional coactivator) as the key transcriptional regulators in the new defense, indicating the dual role of Dicer at the post-transcriptional and transcriptional levels. These findings will bring about a paradigm shift in RNAi. Fungi are now being established as the third position next to animals and plants in antiviral defense research.

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  • Neo-virology: the raison d'etre of viruses

    Grant number:16H06429  2016.06 - 2021.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Kawoaka Yoshihiro

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    Grant amount:\122980000 ( Direct expense: \94600000 、 Indirect expense:\28380000 )

    We have established a new academic field, designated as ‘Neo-virology’, in which we define a virus as a component of the global ecosystem and aim to elucidate its key roles in host organisms and the global ecosystem. We have constructed a system to support research activities, facilitate collaborations among research groups, develop human resources, plan and manage scientific meetings, and support public relations activities, all of which have resulted in accelerating this new field of research. Our efforts have produced numerous noteworthy publications and have helped develop many young investigators, who will continue the growth of Neo-Virology into the future.

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  • 「ネオウイルス学」の国際活動支援

    Grant number:16K21723  2016.06 - 2021.03

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    河岡 義裕, 朝長 啓造, 澤 洋文, 高橋 英樹, 川口 寧, 渡辺 登喜子, 松浦 善治, 鈴木 信弘, 長崎 慶三

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    Grant amount:\46800000 ( Direct expense: \36000000 、 Indirect expense:\10800000 )

    国際活動支援班では、各研究班の海外における研究活動を促進するために、 (1) 国際研究ネットワークの整備および強化、(2) 円滑な海外での研究試料の採取支援・提供、(3) 国際シンポジウムの開催および海外研究者の招聘による人的連携の促進、(4) サンプル収集のための海外への研究者の派遣の支援、(5) 最先端研究を行う先進諸国への若手研究者の中長期的派遣の支援、(6) 国際研究者ネットワークへの積極的な情報発信を行なっている。
    2019年度は、国内で国際学会を4回 [Thermophiles国際学会、第18回あわじ感染と免疫国際フォーラム、日本ウイルス学会学術集会、Asian Mycological Congress 2019]、また、ワークショップを3回 [ウイルス情報解析ワークショップ、Thermophiles Workshop、ネオウイルス学ワークショップ]を共催して、海外から総計10名の研究者を招聘した。招聘した研究者と計画研究班、および公募研究班班員は学会後も共同研究を継続しており、人的連携を構築した。
    また、南米のボリビア共和国に生息する節足動物の採集とウイルス調査を目的として、計画研究班の若手研究者を2名派遣して、サンプルの収集を実施させた。本活動には、計画研究班からさらに2名の研究者も参加して、蚊、ダニを採集した。また、本活動によるガブリエル・レネ・モレノ国立自治大学のJuan Antonio Pereira博士との共同研究に基づいて、計画研究班の若手研究者の研究室に所属する大学院生が、文部科学省の官民協働海外留学創出プロジェクト「トビタテ!留学JAPAN」を活用し、ガブリエル・レネ・モレノ国立自治大学に短期留学を実施し、現地での共同研究を推進した。
    2018年度に引き続き、本領域のホームページの英語版を通して国際社会に本領域の活動を発信している。

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  • A new virus lifestyle: a ss(+)RNA virus hosted by a dsRNA virus

    Grant number:15F15391  2015.11 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows  Grant-in-Aid for JSPS Fellows

    鈴木 信弘, ALAM MD.

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    Grant amount:\2300000 ( Direct expense: \2300000 )

    [yado-kari virus 1のyado-nushi virus1への複製依存性と宿主との相互作用]
    5) 粒子トランスフェクション法によるYnV1単独感染株、混合感染株を作出した。YnV1には3つの変異型が確認されており、それぞれについて単独感染株、混合感染株を作製することに成功した。6) YkV1感染性完全長cDNAの作製し、YnV1単独感染株に導入することでYkV1の「ヤドカリ性」を証明する(RNA Virophageの証明)。7) 感染性クローンが得られたので、各種変異株(RdRpモティーフ、ポリプロテイン切断部位等)を作成し、それらのYkV1複製能への影響を調べた。その結果、RNA依存RNA合成酵素の触媒残基GDDの欠失あるいは置換変異株ではYkV1の複製能は消失した。また、2A-likeプロテアーゼの触媒残基の置換変異株でも同様の傾向が認められた。これは非常に大きな成果と言える。8) 白紋羽病菌と分類上目が異なるクリ胴枯病菌(モデル糸状菌宿主)での複製能を調べた。これまでのところ、陰性の結果が得られた。クリ胴枯病菌の免疫不全株で試したが、陰性であった。

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  • Exploration of infection mechanism and utilization of a mitochondrially replicating virus

    Grant number:15K14663  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    SUZUKI Nobuhiro

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    Grant amount:\4030000 ( Direct expense: \3100000 、 Indirect expense:\930000 )

    Mitoviruses are a unique group of mitochondrially replicating viruses with positive-sense, single stranded RNA genomes. Among them is Cryphonectria mitovirus 1 (CpMV1) which infects one of the three most destructive tree pathogens, chestnut blight fungus. This pathosystem involves two host/parasite interactions (chestnut vs. chestnut blight fungus) and chestnut blight fungus vs. CpMV1). This study represents the first example in which the host range, lateral transmissibility, symptom expression and counter-defense mechanism of a mitovirus was thoroughly analyzed.

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  • Virome of the phytopathogenic fungi: metagenomic analysis and the possible utilization

    Grant number:15K07312  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Kodno Hideki

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    Grant amount:\5070000 ( Direct expense: \3900000 、 Indirect expense:\1170000 )

    The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. However, virus discovery in obligate biotrophs such as powdery mildews (ascomycetes) and rust fungi (basidiomycetes) is still very limited. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least nine novel totivirus (genus Totivirus) in this fungus. Similar totvirus-like sequences are found in public transcriptome shotgun assembly (TSA) libraries of the bean rust fungi. Our data suggests that the horizontal transmission of ancestral totiviruses might have occurred across quite different fungal phyla, between powdery mildews and rust fungi and later evolved within the new host fungus separately.

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  • 白紋羽病菌のヴァイロコントロールに向けた新規2分節dsRNAの性状解析

    Grant number:13F03083  2013.04 - 2015.03

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, JAMAL Atif

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    Grant amount:\2300000 ( Direct expense: \2300000 )

    白紋羽菌、Rosellinia necatrixは日本の果樹に重大な被害を齎している植物糸状菌病である。本研究では、白紋羽菌に感染し、その病原力を衰退させる新規ウイルス(Rosellinia necatrix megabirnavirus 1, RnMBV1)のウイルス学的性状とヴァイロコントロール(生物防除)因子としての潜在力を調べ、マイコウイルス学、ヴァイロコントールへの貢献を目指し、以下の成果を記述する。1-1ウイルス粒子トランスフェクション法を利用し、菌細胞質不和合性の複数の白紋羽病菌への感染性ならびに病原力低下効果を確認した。
    1-2 RnMBV1を目が異なるクリ胴枯病菌への感染性ならびに病原力低下効果を確認した。RnMBV1のヴァイロコントロール因子としての可能性を広げる成果である(JAMAL氏が来日する以前に前倒しで実施し、論文掲載にこぎ着けた)。
    1-3ヴァイロコントロール因子の長期安定保存が不可欠であるが、精製RnMBV1粒子は -80Cで1年以上安定に感染性を保持していた。 2-1 RnMBV1ゲノムセグメントはそれぞれ極めて長い(約1.6 kb)の非翻訳領域(ミニシストロンを複数含む)と2つのORFをもつ。5’側ORFがどのような機構で完全長mRNAから翻訳されるかを、リポーター系を確立した。
    2-2 5’非翻訳領域がIRES(内部リボゾームエントリー部位)活性を持つことを確認し、さらにそのコア活性領域をORF1領域近傍の500塩基の絞り込みに成功した。
    2-3感染性RnMBV1核酸の合成を試みたが、成功には至らなかった。

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  • Development of Neurospora crassa as a model filamentous fungus for studying virus/host interactions.

    Grant number:24658041  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    SUZUKI Nobuhiro

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    As a model system for genetics, biochemistry or developmental biology, Neurospora crassa has greater advantages over any other filamentous fungi due to its even short life cycle time, ease of crossing, and well-established tools and resources represented by a number of gene knockout strains available from the Fungal Genetics Stock Center. This study aimed at screening of previously reported fungal viruses for N. crassa-infectious viruses. We confirmed that N. crassa supports the replication of two heterologous viruses, mycoreovirus 1 (MyRV1) and Rosellinia necatrix partitivirus 2 (RnPV2) from other ascomycetes, Cryphonectrial parasitica and R. necatrix. The two viruses could be maintained not only in the wild-type strain but also RNA silencing defective mutants of N. crassa. It is now being investigated what effects of deletions of RNA silencing-related genes on virus replication are, and how these genes respond to virus infection.

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  • Endogenization process of non-retroviral RNA virus elements into plant genomes and their pathological significance

    Grant number:24580064  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    KONDO Hideki, SUZUKI Nobuhiro, MARUYAMA Kazuyuki

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    Grant amount:\5590000 ( Direct expense: \4300000 、 Indirect expense:\1290000 )

    Endogenous non-retroviral RNA virus-like sequences (NRVSs) have been discovered in the genome of the vertebrates and other eukaryotes. These are considered as fossil RNA viral elements integrated into host genomes by as-yet-known mechanisms. In this study, several novel NRVSs from the genome of the plants, insects and fungi were discovered. The presence of benyvirus-like sequences in the chickpea chromosomes is a second example of plant NRVSs related to positive-sense (+)ssRNA viruses. Benyvirus-related sequences were also found in the chromosomes of blood-sucking insect. In addition, the first (-)RNA virus infection in fungus was evidenced based on a discovery of mononegavirus L-like sequences in the genome of the powdery mildew fungus. Our findings may provide novel insights into the origin and evolution of ssRNA viruses. The possible endogenization process of these NRVSs into the genome and its biological significance on the host were discussed.

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  • ハイポウイルスの多機能性蛋白質p29により誘導されるレオウイルスゲノム再編成

    Grant number:11F01085  2011 - 2013

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, COPE A.E.

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    Grant amount:\1500000 ( Direct expense: \1500000 )

    クリ胴枯病菌(Cryphonectria parasitica)は子のう菌の一種でクリ樹を枯死させる植物病原菌である。クリ胴枯病菌は少なくとも5科のメンバーの宿主となっている。本研究では、異種ウイルス(ハイポウイルス)の蛋白質(p29)で誘発可能なレオウイルス(MyRV1)ゲノムセグメントの遺伝子再編成を多方向から解析した。今年度は上記目的のため以下の3項目を実施した(申請書に記載した実施項目3,4の実施状況については昨年度のC-7-1を参照されたい)。
    5p29のmiRNAへの影響
    糸状菌ではsiRNAは同定されているが、miRNAは未だ発見されていない。そこで、miRNAのクローニングを試みた。しかし、それらの同定には至らなかった。但し、これを相殺する成果として、アカパンカビあるいは植物でsiRNAの増幅に関与すると考えられるRNA依存RNA合成酵素遺伝子(rdr1)の破壊株を得るのに成功し、破壊株では再編成誘導が起らないことを明らかにすることに成功した。
    6植物ウイルス由来RNAサイレンシング抑制蛋自質の遣伝子属編成誘導能
    RNAサイレンシング抑制能と遺伝子再編成誘導能との関連が示された場合は、他のRNAサイレンシング抑制蛋白質(例えば、植物ウイルス由来のPotyvirus HC-Pro, cucumovirus 2b, tombusvirus p19)のMyRV1遺伝子再編成誘導能を検定した。しかし、結果は陰性であった。

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  • Studies on the mechanism of viroplasm formation during plant negative-strand RNA virus infection

    Grant number:21580056  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    KONDO Hideki, SUZUKI Nobuhiro, MARUYAMA Kazuyuki, IDA Bagus andika

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    Grant amount:\4810000 ( Direct expense: \3700000 、 Indirect expense:\1110000 )

    Orchid fleck virus(OFV), a unique two-segmented negative-sense RNA virus, induces an intranuclear electron-lucent viroplasm(inclusion) in infected plant cells. We studied the molecular mechanism by which OFV viroplasms are produced in vivo. Transiently expression examinations by Agrobacterium-infiltration reveled that coexpression of nucleocapsid protein N and the putative phosphoprotein P, in the absence of virus infection, was sufficient for the formation of an intranuclear electron-lucent viroplasm-like structure in Nicotiana benthamiana cells.

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  • Genome transport mechanisms of plant negative-stranded RNA virus

    Grant number:19580048  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    KONDO Hideki, SUZUKI Nobuhiro

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    植物のマイナス鎖RNAウイルスであるOFVをモデルとし、ゲノム輸送に関わると考えられるウイルス因子を解析した。特にウイルスの移行形態とされるvRNP複合体を精製し、構成ウイルス蛋白質を明らかにした。そのうち、ゲノム結合タンパク質であるNの細胞核(複製の場と推定)への輸送にはアクセサリー蛋白質であるPが必要であることを発見した。また、モデル植物における細胞間移行や長距離移行に関する一連の研究も行った。これらの成果は、OFVのゲノム移行輸送機構を理解する上で基礎的な知見となると期待される。

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  • 根におけるRNAサイレンシング調節機構の解明とウイルス抵抗性植物創成への応用

    Grant number:07F07157  2007 - 2008

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, ANDIKA I.

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    Grant amount:\2200000 ( Direct expense: \2200000 )

    本研究では,「根でのRNAサイレンシング活性に関与する宿主因子とそれを低減する因子」を解析した.まず,第一にトランスジーンやウイルスに対するサイレンシング活性が根で低いという現象の一般化を検討した.すなわち,数種のウイルスと宿主植物の組み合わせで調べ,根におけるウイルスのサイレンシング活性抑制効果についても詳細に解析した.次に,トランスジーン,ウイルス由来のsiRKAの根での蓄積量が低かったことから,その産生に関与するDCL群などの宿主因子を中心に,アラビドプシスの葉と根での発現パターンを解析した.さらに,候補となる宿主因子の変異体について,トランスジーンやウイルス等に対する影響を評価した.
    1.[根でのRNAサイレンシングにより影響される複製レベルはウィルスにより異なる]
    トランスジーンに対するサイレンシングが根で低いことは植物種を越えて一般化できるが,ウイルスに対するサイレング効果はウイルス種により異なっていた.
    2.[RNAサイレンシング関連遺伝子の変異体とウイルス感受性]
    PVXをアラビドプシス(非宿主とされていた)のdcl変異体へ接種したところ,DCL2とDCL4の変異体で効率的に全身移行したが,根ではDCL2,DCL4に加えDCL3もPVXの蓄積に影響することが示唆された.
    3.[サイレンシング関連遺伝子変異体における内在型siRNAの蓄積とトランスジーンサイレンシングへの影響]
    現在実施中.
    4.[サイレンシング活性低減要因(遺伝子)の根における強制的発現ラインの作出]
    活性の低減に関わる候補遺伝子BNYVV p14をN.benthamiana根で強制的に発現させた.

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  • Analysis of RNA silencing suppressor activities of viral proteins in a chestnut blight fungus system

    Grant number:14360030  2002 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    SUZUKI Nobuhiro

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    Grant amount:\14400000 ( Direct expense: \14400000 )

    Post transcriptional gene silencing(PTGS), first discovered in plants independently as cosuppresion and RNA-mediated virus resistance, is a sequence-specific RNA degradation system which is now believed to be ubiquitous in eukaryotic organisms from fungi to mammals. Double-stranded RNA is key molecules triggering PTGS and being digested by RNaseIII type nuclease to 22-25 nucleotides (siRNA, a hall mark of PTGS) which guide RISC (RNA inducing silencing complex, AGO family member being a major component) to target RNA and lead to the degradation of the RNA. PTGS is conceptualized as an anti-virus host defense mechanism by several lines of evidence. As its counterattack strategy plant and animal viruses as well encode PTGS suppressors which seem to act in different ways. Most of them are derived from positive and negative strand RNA or DNA viruses. Here in this project dsRNA viruses were subjected to anti-PTGS activity assay in a filamentous fungus, Cryphonectria parasitica (the chestnut blight fungus) and animal. Proteins tested included those encoded by the prototype hypovirus, CHV1-EP713, two plant reoviruses, dicot plant-infecting wound tumor virus(WTV) and monocot plant-infecting rice dwarf virus(RDV), the latter two of witch are also insect (animal)-infecting.
    1)Some RNA silencing suppressors are known to enhance replication of heterologous viruses. Here suppressive activities of candidate proteins of different virus origins were evaluated based on the levels of MYRV1, a member of a newly established genus within the family Reoviridae. By this assay the papain-like proteinase p29 derived from the prototype hypovirus (CHV1), was shown to elevate the replication and vertical transmission levels of MYRV1, indicative the RNA silencing suppressive activity of p29, while no such activity by p40 of CHV1, reported to augment the homologous virus replication, was not found. Viral proteins encoded by plant- and insect-infecting reoviruses, i.e., rice dwarf phytoreovirus and wound tumor virus were tested. However, no elevation of MYRV1 replication was found by those proteins.
    2)Trangenic C parasitica lines silenced for Hygromycin resistance gene(HygR) were attempted to be generated. To this end, C parasitica spheroplasts were transformed with HygR under the control of constitutive promoter. Resulting transgenic lines were doubly transformed with an inverted repeat of HygR with an intron spacer. Out of more than 200 independent transformants, approximately 10-20 showed Hyg succeptible, probably because of RNA silecing triggered by the dsRNA form of HygR. These isolates were being used for RNA silencing suppressor assay in which the candidate proteins as above are examined for the ability to reverting their phenotype to HygR.

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  • イネ萎縮ウイルス複製酵素複合体の再構成

    Grant number:07856005  1995

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

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    Grant amount:\900000 ( Direct expense: \900000 )

    イネ萎縮ウイルスはファイトレオウイルスんい属するウイルスで、12本の文節2本鎖RNAをゲノムに持つ。昨年全ゲノムの構造解析が完了し(植物レオウイルスとしては最初である)、各ゲノムが4塩基の末端保存配列、逆反復配列、単一のORFから構成されることが示された。末端保存配列、逆反復配列の末端配列ドメインはゲノムセグメントの粒子への詰め込みと複製・転写に関与することが示唆されているが、明らかとなっていない。本研究では末端保存配列と相互作用するであろうウイルス蛋白質、宿主蛋白質の同定、さらにそれら蛋白質と相互作用するシス配列の同定を最終目的に、RNA合成活性を持つRDVコア粒子の再構成を試みた。
    RDVのコア粒子を構成する蛋白質遺伝子全て(S1、S3、S5 S7)を昆虫(夜盗蛾)細胞、Spodoptera frugiperdaで発現させることに成功した。さらに疑似コア粒子と考えられる構造物の部分精製も行った。但し、現在までのところ、この構造物のRNA合成活性は確認されていない。今後、コア粒子様構造物の精製法、鋳型導入法等の検討を要する。
    研究過程でRDV複製に関与するであろう酵素について興味深い発見をした。以下に記す。これまで、P2、P5はアウタ-レイヤー蛋白質と考えられていたが、昨年度の奨励研究Aによりマイナ-コア蛋白質をコードすることが明らかとなった。本研究によりこのP5がGTPと共有結合することが示された。P5はmRNA転写の際のキャッピング酵素、すなわちグアニル酸転移酵素あるることが示唆された。また、P5はGTPのほかにATP,UTPとの結合活性を持っていた。この活性はこれまで報告されているグアニル酸転移酵素とは大きく異なる性質である。ATP,UTP,GTPの結合サイトは同じか?、ATP,UTP結合能の生物学的意義は何か?等、今後の大きな検討課題である。

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  • イネ萎縮ウイルスのヨコバイ細胞感染性を支配するウイルス因子の同定

    Grant number:06856008  1994

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

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    Grant amount:\900000 ( Direct expense: \900000 )

    1)他の植物レオウイルスで得られた過去の結果と予備実験の結果から、S2、S5がウイルス粒子表面に存在する蛋白質(P2、P5)をコードすること、さらにこれらがウイルス精製過程で脱落するすることが示唆された。まず、これをP2、P5に対する抗体を用いて証明した。
    RDV S2(一部)、S5(完全長)のcDNAをそれぞれ夜盗蛾培養細胞中で発現させた。P2のカルボキシル基末端半分(60 kDa)と89kDaのP5の大量発現が確認された。これらのRDV由来の蛋白質をウサギに免疫し、抗血清を得た。免疫電顕法を用いてこれらの抗体と粒子との反応性を調べた結果、P2血清は粒子と反応したが、P5血清は反応しなかった。次にウエスタン法を用いて精製粒子、精製コア(内殻)粒子との反応性を調べた。P2血清は精製粒子中の130 kD蛋白質と反応したが、コア粒子中には反応する蛋白質は検出されなかった。一方、P5血清は精製粒子中の89kDマイナ-蛋白質と反応した。また、この蛋白質はP5抗体によってコア粒子からも微量検出された。
    これらの結果から、P2は予想された通り粒子の外殻粒子に存在するがP5については外殻蛋白質ではなく内殻粒子を構成する微量成分であることが明らかになった。S5については従来S2と同様に外殻蛋白質をコードすると考えられてきた。しかし、興味深いことにコア蛋白質をコードすることが証明された。植物レオウイルスの中で遺伝子-産物の全ての対応が明らかとなったのはRDVが初めてであり、世界的に評価されうる大きな成果である。
    従って、P2とこれまでに外殻蛋白質と同定されているP8がヨコバイ培養細胞感染性を支配することが示唆された。
    2)ヨコバイ培養細胞株の樹立。
    木村の方法(1987年)によってツマグロヨコバイ卵細胞の株化を試みた。初代培養には成功したが、安定継代株はえられていない。
    今後、安定なヨコバイ培養細胞株を樹立し、P2・P8抗体を用いたRDVの感染中和試験を行う必要がある。また、RDVS2コードのP2を完全な状態で組み換えバキュロウイルス発現夜盗蛾細胞から精製し、その精製P2を用いて完全粒子の再構成系を確立する必要がある。

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  • 虫媒伝染性欠損に関与するイネ萎縮ウイルスの同定

    Grant number:05856006  1993

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

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    Grant amount:\900000 ( Direct expense: \900000 )

    (1)イネ萎縮ウイルス(RDV)はヨコバイで永続的に媒介され、しかも虫体内で増殖し高率に経卵伝染する。しかし、本ウイルスを数年イネ植物体だけで継代することによって(感染イネを株分けで栽培し続ける)、伝染性が失われるという興味深い現象が見いだされる。本研究ではまず、この伝染性が欠落したRDV感染イネを得た。
    (2)野性型とヨコバイ伝染性欠損型RDVでゲノムセグメントの電気泳動像に違いがあるかどうかまず調べた結果、両者に差は認められなかった。
    (3)RDVゲノムセグメントがコードする蛋白質をバキュロウイルスベクターを用いて発現させ、それらに対する抗体を作製した。
    (4)3)で作製した抗体を用いて野性型、伝染性欠損型感染イネ中のRDV蛋白質の検出を行なった。イネ個体間で検出量に差は認められたが、野性型と欠損型との間では明確な違いは認められなかった。
    これらの結果は、他のレオウイルスで報告されているような特定セグメントの欠失によるのではなく、他のメカニズム(例えば点突然変異)によってRDVのヨコバイ伝染性欠損が起こることを示唆する。

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  • ライボザイムを利用したイネ委縮病の防除

    Grant number:04856011  1992

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

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    Grant amount:\900000 ( Direct expense: \900000 )

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  • イネのロイシンジッパ-型DNA結合タンパク質の遺伝子構造と機能

    Grant number:03262215  1991 - 1993

    日本学術振興会  科学研究費助成事業 重点領域研究  重点領域研究

    草野 友延, 鈴木 信弘

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    Grant amount:\1900000 ( Direct expense: \1900000 )

    我々は,種々の環境ストレスのうち低温(5℃)がイネ植物に及ぼす影響を遺伝子発現のレベルの違いで検出しようと研究を進めてきた。独自に開発したcDNAライブラリ-作成法及びサブトラクション法〔Nucleic Acids Res.18:1071(1990),Plaut Cell Physiol.32:in press(1991)〕により低温処理で特異的に誘導される3種のイネcDNAクロ-ンの分離に成功してきた。これら3種のクロ-ンは、低温処理との関連が示唆されているアブシジン酸の処理あるいは高浸透圧(0.5Mマニト-ル)処理によっては誘導はおこらず,また熱ショク処理によっても誘導されない。更には1度低温で誘導されたものを常温25℃に戻してやると転写レベルでの誘導が全くおこっていない事も確認している。従ってこれらのクロ-ンは低温処理により特異的に発現が誘導されると結論した。
    これらのクロ-ンのうちLip19と命名したものは1323塩基対からなり,1次構造解析及びセンス鎖の同定などより約19,000の分子量のタンパク質をコ-ドし,その遺伝子産物はタンパク質デ-タベ-ス検索の結果トウモロコシ由来のジッパ-モチ-フをもつDNA結合タンパク質であることが明らかになっている。
    ゲノミックサザン法によりlip19遺伝子は1コピ-であることが判明した。λgtloの部分ライブラリ-より,ゲノミッククロ-ンを4kbのEcoRI断片として単離した。物理地図及び1次構造の解析により,lip19は翻訳領域,非翻訳領域を問わず挿入配列をもたないことが明らかとなった。現在,E.ColiのTクポリメラ-ゼ/Tクプロモ-タ-発現系で作らせたLip19を用い,DNA認識配列の同定を行っている。

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  • イネ委縮ウイルスmRNAのイネおよび昆虫培養細胞への導入

    Grant number:02856013  1990

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

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    Grant amount:\900000 ( Direct expense: \900000 )

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  • イネ萎縮ウイルスの遺伝的解析

    Grant number:01760048  1989

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

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    Grant amount:\900000 ( Direct expense: \900000 )

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  • Seminar in Molecular Virology (2024academic year) Prophase  - その他

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  • Plant-Virus/Bacteria Interactions (2024academic year) Prophase  - 木5~8

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  • Plant-Virus/Bacteria Interactions (2023academic year) Prophase  - 木5~8

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Social Activities

  • ミクロの世界へようこそ、ウイルス学入門

    Role(s):Lecturer

    岡山大学資源植物科学研究所  Summer Science School SSS2021  2021.8.2

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  • ウイルスと生きる:SARS-CoV-2.

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    岡山大学資源植物科学研究所  Summer Science School online 2020  2020.11.21

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  • 今だから聞けるウイルス学講座「菌類ウイルスに魅せられて」

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    ウイルス学会  湯河原ウイルスキャンプ  2018.6.6 - 2018.6.7

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  • 岡山大学先端研究講座 ネオウイルス学:ウイルスと生きる

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    岡山大学  岡山大学先端研究講座  2017.6.17

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  • 研究室紹介

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    高校生研究所訪問  2017.3.14

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  • ウイルスの有効利用〜ウイルスで病気を制す:「ヴァイロコントール」〜

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    岡山大学知恵の見本市2016  2016.11.11

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  • これでもウイルス?

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    独立行政法人国立病院機構仙台医療センター  みちのくウイルス塾  2016.7.16 - 2016.7.17

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  • 職場体験

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    岡山大学資源植物科学研究所  2013.10.16 - 2013.10.19

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  • 日本学術振興会(JSPS)サイエンス・ダイアログ プログラム

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    日本学術振興会(JSPS)  2013.2.14

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  • マイコウイルスとヴァイロコントロール

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    日本植物病理学会  平成24年度植物感染生理談話会  2012.8.30

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  • ウイルスいろいろ:目立たないウイルス、植物の守り神となるウイルス

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    独立行政法人国立病院機構仙台医療センター  みちのくウイルス塾  2012.7.14 - 2012.7.15

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  • ウイルスいろいろ:役立つウイルス、目立たないウイルス

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    岡山大学資源植物科学研究所  平成24年度 公開講座「ウイルス -摩訶不思議な不完全生命体-」  2012.5.26

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  • ヴァイロコントロール:悪玉カビを病気にする善玉ウイルス

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    日本ウイルス学会  ウイルス学キャンプ  2010.8.9 - 2010.8.10

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  • 病害虫防除の最前線」植物の病気の生物防除=ウイルスをもってカビを制す=

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    山陽放送  吉備創生カレッジ講座  2009.6.27

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  • 賢いウイルスとその遺伝子

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    岡山大学  岡山大学公開講座  2002.7

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