2024/03/07 更新

写真a

スズキ ノブヒロ
鈴木 信弘
SUZUKI Nobuhiro
所属
資源植物科学研究所 教授
職名
教授
プロフィール

Dr. Nobuhiro SUZUKI received his M. S. (1985) in phytopathology and Ph. D (1989) in virology from Tohoku University in Sendai, Japan. Dr. SUZUKI currently serves as a full professor of the Institute of Plant Stress and Resources, formerly Institute of Plant Sciences and Bioresouces at Okayama University and as an Editor/Board member of Virus Research, Frontiers in Virology, Journal of General Plant Pathology, Virology Journal, Biology, Journal of Virology, and Virology. He has also been Guest Editors to PLoS Pathogens, PNAS, and mBio.

Suzuki Laboratory focuses on characterization of diverse viruses infecting phytopathogenic fungi and exploration of their interplays. Recent achievements include the discovery of a neo-virus lifestyle exhibited by a (+)ssRNA virus and an unrelated dsRNA virus in a plant pathogenic fungus. Prior to coming to Kurashiki, Okayama Prefecture, he was a visiting fellow of the Center for Agricultural Biotechnology at the University of Maryland Biotechnology Institute for four years (1997-2001) to study molecular biology of hypoviruses in the laboratory of Professor Donald L. Nuss. Before visiting UMBI, he served as an assistant professor and a lecturer of the Biotechnology Institute at the Akita Prefectural College of Agriculture for 11 years (1988-1998) where he conducted a project on molecular characterization of rice dwarf phytoreovirus, a member of the family Reoviridae. He received awards from the Japanese Phytopathological Society of Japan and Japanese Society for Virology for his outstanding achievements in plant virology.

外部リンク

学位

  • 博士(農学) ( 東北大学 )

研究キーワード

  • 植物ウイルス

  • RNAサイレンシング

  • 菌類ウイルス

  • Virology

  • ウイルス学

  • 生物防除

  • ウイルス防御

研究分野

  • 環境・農学 / 植物保護科学

学歴

  • 東北大学    

    - 1989年

      詳細を見る

    国名: 日本国

    researchmap

  • 東北大学   Graduate School, Division of Agriculture  

    - 1989年

      詳細を見る

  • 東北大学   Faculty of Agriculture  

    - 1983年

      詳細を見る

    国名: 日本国

    researchmap

  • 東北大学   Faculty of Agriculture   Agronomy

    - 1983年

      詳細を見る

経歴

  • Swiss Federal Research Institute WSL   Forest Health & Biotic Interactions   WSL フェロー

    2019年6月 - 2019年11月

      詳細を見る

    国名:スイス連邦

    researchmap

  • 岡山大学資源植物科学研究所 教授   植物・微生物相互作用グループ   教授

    2007年4月 - 現在

      詳細を見る

  • 岡山大学資源生物科学研究所   植物・微生物相互作用グル ープ   助教授

    2001年4月 - 2007年3月

      詳細を見る

  • メリーランド州立大学生物工学研究所   農生物工学研究所   客員助教授

    1998年4月 - 2001年3月

      詳細を見る

  • メリーランド州立大学生物工学研究所   農生物工学研究所   客員研究員

    1996年4月 - 1997年3月

      詳細を見る

  • 秋田県立大学短期大学部   生物工学研究所   講師

    1994年4月 - 1998年3月

      詳細を見る

  • 農林水産省農業研究センター   客員研究員

    1994年4月 - 1996年3月

      詳細を見る

  • 秋田県立大学短期大学部   生物工学研究所   助手

    1988年5月 - 1990年3月

      詳細を見る

▼全件表示

所属学協会

委員歴

  • 国際ウイルス分類委員会   国際ウイルス分類委員会 役員  

    2020年10月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • Biology -Basel-   Editor  

    2019年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • PLoS Pathogens   Guest Editor  

    2017年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • Journal of Virology   Editorial Board Member  

    2016年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • Virology   Editorial Board Member  

    2015年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • Proceedings of the National Academy of Science, U. S. A.   Guest Editor  

    2014年1月 - 2015年6月   

      詳細を見る

    団体区分:学協会

    researchmap

  • Frontiers in Virology   Editor  

    2011年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • Virus Research   Editor  

    2010年12月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • Viruses   Editorial Board Member  

    2022年9月 - 現在   

      詳細を見る

    団体区分:その他

    researchmap

  • 日本ウイルス学会   理事  

    2022年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • Editor   Archives of Virology  

    2022年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • ICTV Study Group of the family Totiviridae   Study Group Chair  

    2020年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • Journal of General Plant Pathology   sub-Editor-in-Chief  

    2020年1月 - 2021年12月   

      詳細を見る

    団体区分:学協会

    researchmap

  • 日本ウイルス学会   学術集会プログラム委員  

    2019年4月 - 2020年3月   

      詳細を見る

  • Virology Journal   Editor  

    2019年1月 - 現在   

      詳細を見る

  • 日本植物病理学会   植物ウイルス分類委員会委員長  

    2018年4月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • 日本植物病理学会   評議委員  

    2018年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • 日本ウイルス学会   選考委員  

    2018年1月 - 2019年12月   

      詳細を見る

    団体区分:学協会

    researchmap

  • Frontiers in Cellular and Infection Microbiology   Guest Editor  

    2018年1月 - 2019年12月   

      詳細を見る

    団体区分:学協会

    researchmap

  • mBio   Guest Editor  

    2017年1月 - 2017年12月   

      詳細を見る

    団体区分:学協会

    researchmap

  • Journal of General Plant Pathology   Editor  

    2016年1月 - 2020年12月   

      詳細を見る

    団体区分:学協会

    researchmap

  • 日本ウイルス学会   理事  

    2016年1月 - 2019年12月   

      詳細を見る

  • 日本ウイルス学会   評議委員  

    2015年1月 - 現在   

      詳細を見る

  • ICTV Study Group of the family Partitivirus   Study Group Member  

    2015年1月 - 2020年12月   

      詳細を見る

    団体区分:学協会

    researchmap

  • ICTV Study Group of the family Hypoviridae   Study Group Chair  

    2015年1月 - 2020年12月   

      詳細を見る

    団体区分:学協会

    researchmap

  • 日本ウイルス学会   学術集会プログラム委員  

    2013年4月 - 2017年3月   

      詳細を見る

  • ICTV Study Group of the family Reoviridae   Study Group Member  

    2012年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • ICTV Study Group of the family Chrysoviridae and Quadriviridae   Study Group Member  

    2012年 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • ICTV Study Group of the family Partitiviridae   Study Group Chair  

    2011年1月 - 2014年12月   

      詳細を見る

    団体区分:学協会

    researchmap

  • 日本ウイルス学会   日本ウイルス学会会誌「ウイルス」編集委員  

    2010年1月 - 2013年12月   

      詳細を見る

  • 日本ウイルス学会   広報委員会委員  

    2009年 - 2012年   

      詳細を見る

    団体区分:学協会

    日本ウイルス学会

    researchmap

  • Virus Research   Editorial Board Member  

    2007年12月 - 2010年11月   

      詳細を見る

    団体区分:学協会

    researchmap

  • 日本植物病理学会   植物ウイルス病研究会委員  

    2004年4月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • 日本植物病理学会   植物ウイルス分類委員会委員  

    2004年4月 - 2018年3月   

      詳細を見る

    団体区分:学協会

    researchmap

  • ICTV Study Group of the family Hypoviridae   Study Group Member  

    2000年1月 - 2014年12月   

      詳細を見る

    団体区分:学協会

    researchmap

▼全件表示

 

論文

  • Proof of concept of the Yadokari nature: A capsidless replicase-encoding but replication-dependent positive-sense single-stranded RNA virus hosted by an unrelated double-stranded RNA virus 査読

    Subha Das, Md Mahfuz Alam, Rui Zhang, Sakae Hisano, Nobuhiro Suzuki

    Journal of Virology   95 ( 17 )   e00467-21   2021年9月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We previously proposed a new virus lifestyle or yadokari/yadonushi nature exhibited by a positive-sense single-stranded RNA (ssRNA) virus, yadokari virus 1 (YkV1), and an unrelated double-stranded RNA (dsRNA) virus, yadonushi virus 1 (YnV1) in a phytopathogenic ascomycete, Rosellinia necatrix. We have proposed that YkV1 diverts the YnV1 capsid to trans-encapsidate YkV1 RNA and RNA-dependent RNA polymerase (RdRp) and replicate in the heterocapsid. However, it remains uncertain whether YkV1 replicates using its own RdRp and whether YnV1 capsid copackages both YkV1 and YnV1 components. To address these questions, we first took advantage of the reverse genetics tools available for YkV1. Mutations in the GDD RdRp motif, one of the two identifiable functional motifs in the YkV1 polyprotein, abolished its replication competency. Mutations were also introduced in the conserved 2A-like peptide motif, hypothesized to cleave the YkV1 polyprotein cotranslationally. Interestingly, the replication proficiency of YkV1 mutants in the host fungus agreed with the cleavage activity of the 2A-like peptide tested using a baculovirus expression system. Cesium chloride equilibrium density gradient centrifugation allowed for the separation of particles, with a subset of YnV1 capsids solely packaging YkV1 dsRNA and RdRp. These results provide proof of concept that a capsidless positive-sense ssRNA [(1)ssRNA] virus is hosted by an unrelated dsRNA virus. IMPORTANCE Viruses typically encode their own capsids that encase their genomes. However, a capsidless positive-sense single-stranded RNA [(1)ssRNA] virus, YkV1, depends on an unrelated double-stranded RNA (dsRNA) virus, YnV1, for encapsidation and replication. We previously showed that YkV1 highjacks the capsid of YnV1 for trans-encapsidation of its own RNA and RdRp. YkV1 was hypothesized to divert the heterocapsid as the replication site, as is commonly observed for dsRNA viruses. Herein, mutational analyses showed that the RdRp and 2A-like domains of the YkV1 polyprotein are important for its replication. The active RdRp must be cleaved by a 2A-like peptide from the C-proximal protein. Cesium chloride equilibrium density gradient centrifugation allowed for the separation of particles, with YnV1 capsids solely packaging YkV1 dsRNA and RdRp. This study provides proof of concept of a virus neo-lifestyle where a (1)ssRNA virus snatches capsids from an unrelated dsRNA virus to replicate with its own RdRp, thereby mimicking the typical dsRNA virus lifestyle.

    DOI: 10.1128/JVI.00467-21

    Scopus

    PubMed

    researchmap

  • Establishment of Neurospora crassa as a model organism for fungal virology 査読

    Shinji Honda, Ana Eusebio-Cope, Shuhei Miyashita, Ayumi Yokoyama, Annisa Aulia, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki

    Nature Communications   11 ( 1 )   5627   2020年12月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The filamentous fungus Neurospora crassa is used as a model organism for genetics, developmental biology and molecular biology. Remarkably, it is not known to host or to be susceptible to infection with any viruses. Here, we identify diverse RNA viruses in N. crassa and other Neurospora species, and show that N. crassa supports the replication of these viruses as well as some viruses from other fungi. Several encapsidated double-stranded RNA viruses and capsid-less positive-sense single-stranded RNA viruses can be experimentally introduced into N. crassa protoplasts or spheroplasts. This allowed us to examine viral replication and RNAi-mediated antiviral responses in this organism. We show that viral infection upregulates the transcription of RNAi components, and that Dicer proteins (DCL-1, DCL-2) and an Argonaute (QDE-2) participate in suppression of viral replication. Our study thus establishes N. crassa as a model system for the study of host-virus interactions.

    DOI: 10.1038/s41467-020-19355-y

    Scopus

    PubMed

    researchmap

  • Hadaka virus 1: A capsidless eleven-segmented positive-sense single-stranded RNA virus from a phytopathogenic fungus, Fusarium oxysporum 査読 国際誌

    Yukiyo Sato, Wajeeha Shamsi, Atif Jamal, Muhammad Faraz Bhatti, Hideki Kondo, Nobuhiro Suzuki, Reed B. Wickner

    mBio   11 ( 3 )   e0045-20   2020年5月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The search for viruses infecting fungi, or mycoviruses, has extended our knowledge about the diversity of RNA viruses, as exemplified by the discovery of polymycoviruses, a phylogenetic group of multisegmented RNA viruses with unusual forms. The genomic RNAs of known polymycoviruses, which show a phylogenetic affinity for animal positive-sense single-stranded RNA [(+)RNA] viruses such as caliciviruses, are comprised of four conserved segments with an additional zero to four segments. The double-stranded form of polymycovirus genomic RNA is assumed to be associated with a virally encoded protein (proline-alanine-serine-rich protein [PASrp]) in either of two manners: a capsidless colloidal form or a filamentous encapsidated form. Detailed molecular characterizations of polymycoviruses, however, have been conducted for only a few strains. Here, a novel polymyco-related virus named Hadaka virus 1 (HadV1), from the phytopathogenic fungus Fusarium oxysporum, was characterized. The genomic RNA of HadV1 consisted of an 11-segmented positive-sense RNA with highly conserved terminal nucleotide sequences. HadV1 shared the three conserved segments with known polymycoviruses but lacked the PASrp-encoding segment. Unlike the known polymycoviruses and encapsidated viruses, HadV1 was not pelleted by conventional ultracentrifugation, possibly due to the lack of PASrp. This result implied that HadV1 exists only as a soluble form with naked RNA. Nevertheless, the 11 genomic segments of HadV1 have been stably maintained through host subculturing and conidiation. Taken together, the results of this study revealed a virus with a potential novel virus lifestyle, carrying many genomic segments without typical capsids or PASrp-associated forms. IMPORTANCE Fungi collectively host various RNA viruses. Examples include encapsidated double-stranded RNA (dsRNA) viruses with diverse numbers of genomic segments (from 1 to 12) and capsidless viruses with nonsegmented (+)RNA genomes. Recently, viruses with unusual intermediate features of an infectious entity between encapsidated dsRNA viruses and capsidless (+)RNA viruses were found. They are called polymycoviruses, which typically have four to eight dsRNA genomic segments associated with one of the virus-encoded proteins and are phylogenetically distantly related to animal (+)RNA caliciviruses. Here, we identified a novel virus phylogenetically related to polymycoviruses, from the phytopathogenic fungus Fusarium oxysporum. The virus, termed Hadaka virus 1 (HadV1), has 11 (+)RNA genomic segments, the largest number in known (+)RNA viruses. Nevertheless, HadV1 lacked a typical structural protein of polymycoviruses and was not pelleted by standard ultracentrifugation, implying an unusual capsidless nature of HadV1. This study reveals a potential novel lifestyle of multisegmented RNA viruses.

    DOI: 10.1128/mBio.00450-20

    Scopus

    PubMed

    researchmap

  • Dicer functions transcriptionally and posttranscriptionally in a multilayer antiviral defense. 査読

    Andika IB, Kondo H, Suzuki N

    Proceedings of the National Academy of Sciences of the United States of America   116 ( 6 )   2274 - 2281   2019年2月

  • SAGA complex mediates the transcriptional up-regulation of antiviral RNA silencing 査読

    Ida Bagus Andika, Atif Jamal, Hideki Kondo, Nobuhiro Suzuki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 17 )   E3499 - E3506   2017年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Pathogen recognition and transcriptional activation of defense-related genes are crucial steps in cellular defense responses. RNA silencing (RNAi) functions as an antiviral defense in eukaryotic organisms. Several RNAi-related genes are known to be transcriptionally up-regulated upon virus infection in some host organisms, but little is known about their induction mechanism. A phytopathogenic ascomycete, Cryphonectria parasitica (chestnut blight fungus), provides a particularly advantageous system to study RNAi activation, because its infection by certain RNA viruses induces the transcription of dicer-like 2 (dcl2) and argonaute-like 2 (agl2), two major RNAi players. To identify cellular factors governing activation of antiviral RNAi in C. parasitica, we developed a screening protocol entailing multiple transformations of the fungus with cDNA of a hypovirus mutant lacking the RNAi suppressor (CHV1-Delta p69), a reporter construct with a GFP gene driven by the dcl2 promoter, and a random mutagenic construct. Screening for GFP-negative colonies allowed the identification of sgf73, a component of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, a well-known transcriptional coactivator. Knockout of other SAGA components showed that the histone acetyltransferase module regulates transcriptional induction of dcl2 and agl2, whereas histone deubiquitinase mediates regulation of agl2 but not dcl2. Interestingly, full-scale induction of agl2 and dcl2 by CHV1 Delta p69 required both DCL2 and AGL2, whereas that by another RNA virus, mycoreovirus 1, required only DCL2, uncovering additional roles for DCL2 and AGL2 in viral recognition and/or RNAi activation. Overall, these results provide insight into the mechanism of RNAi activation.

    DOI: 10.1073/pnas.1701196114

    Web of Science

    researchmap

  • Harnessing host ROS-generating machinery for the robust genome replication of a plant RNA virus 査読

    Kiwamu Hyodo, Kenji Hashimoto, Kazuyuki Kuchitsu, Nobuhiro Suzuki, Tetsuro Okuno

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 7 )   E1282 - E1290   2017年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    As sessile organisms, plants have to accommodate to rapid changes in their surrounding environment. Reactive oxygen species (ROS) act as signaling molecules to transduce biotic and abiotic stimuli into plant stress adaptations. It is established that a respiratory burst oxidase homolog B of Nicotiana benthamiana (NbRBOHB) produces ROS in response to microbe-associated molecular patterns to inhibit pathogen infection. Plant viruses are also known as causative agents of ROS induction in infected plants; however, the function of ROS in plant-virus interactions remains obscure. Here, we show that the replication of red clover necrotic mosaic virus (RCNMV), a plant positive-strand RNA [(+) RNA] virus, requires NbRBOHB-mediated ROS production. The RCNMV replication protein p27 plays a pivotal role in this process, redirecting the subcellular localization of NbRBOHB and a subgroup II calcium-dependent protein kinase of N. benthamiana (NbCDPKiso2) from the plasma membrane to the p27-containing intracellular aggregate structures. p27 also induces an intracellular ROS burst in an RBOH-dependent manner. NbCDPKiso2 was shown to be an activator of the p27-triggered ROS accumulations and to be required for RCNMV replication. Importantly, this RBOH-derived ROS is essential for robust viral RNA replication. The need for RBOHderived ROS was demonstrated for the replication of another (+) RNA virus, brome mosaic virus, suggesting that this characteristic is true for plant (+) RNA viruses. Collectively, our findings revealed a hitherto unknown viral strategy whereby the host ROS-generating machinery is diverted for robust viral RNA replication.

    DOI: 10.1073/pnas.1610212114

    Web of Science

    PubMed

    researchmap

  • A capsidless ssRNA virus hosted by an unrelated dsRNA virus 査読

    Rui Zhang, Sakae Hisano, Akio Tani, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    Nature Microbiology   1 ( 1 )   2016年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nature Publishing Group  

    Viruses typically encode the capsid that encases their genome, while satellite viruses do not encode a replicase and depend on a helper virus for their replication 1. Here, we report interplay between two RNA viruses, yado-nushi virus 1 (YnV1) and yado-kari virus 1 (YkV1), in a phytopathogenic fungus, Rosellinia necatrix2. YkV1 has a close phylogenetic affinity to positive-sense, single-stranded (+)ssRNA viruses such as animal caliciviruses3, while YnV1 has an undivided double-stranded (ds) RNA genome with a resemblance to fungal totiviruses4. Virion transfection and infectious full-length cDNA transformation has shown that YkV1 depends on YnV1 for viability, although it probably encodes functional RNA-dependent RNA polymerase (RdRp). Immunological and molecular analyses have revealed trans-encapsidation of not only YkV1 RNA but also RdRp by the capsid protein of the other virus (YnV1), and enhancement of YnV1 accumulation by YkV1. This study demonstrates interplay in which the capsidless (+)ssRNA virus (YkV1), hijacks the capsid protein of the dsRNA virus (YnV1), and replicates as if it were a dsRNA virus.

    DOI: 10.1038/nmicrobiol.2015.1

    Web of Science

    Scopus

    PubMed

    researchmap

  • Highly activated RNA silencing via strong induction of dicer by one virus can interfere with the replication of an unrelated virus 査読

    Sotaro Chiba, Nobuhiro Suzuki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   112 ( 35 )   E4911 - E4918   2015年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Viruses often coinfect single host organisms in nature. Depending on the combination of viruses in such coinfections, the interplay between them may be synergistic, apparently neutral with no effect on each other, or antagonistic. RNA silencing is responsible for many cases of interference or cross-protection between viruses, but such antagonistic interactions are usually restricted to closely related strains of the same viral species. In this study, we present an unprecedented example of RNA silencing-mediated one-way interference between unrelated viruses in a filamentous model fungus, Cryphonectria parasitica. The replication of Rosellinia necatrix victorivirus 1 (RnVV1; Totiviridae) was strongly impaired by coinfection with the prototypic member of the genus Mycoreovirus (MyRV1) or a mutant of the prototype hypovirus (Cryphonectria hypovirus 1, CHV1) lacking the RNA silencing suppressor (CHV1-Delta p69). This interference was associated with marked transcriptional induction of key genes in antiviral RNA silencing, dicer-like 2 (dcl2) and argonaute-like 2 (agl2), following MyRV1 or CHV1-Delta p69 infection. Interestingly, the inhibition of RnVV1 replication was reproduced when the levels of dcl2 and agl2 transcripts were elevated by transgenic expression of a hairpin construct of an endogenous C. parasitica gene. Disruption of dcl2 completely abolished the interference, whereas that of agl2 did not always lead to its abolishment, suggesting more crucial roles of dcl2 in antiviral defense. Taken altogether, these results demonstrated the susceptible nature of RnVV1 to the antiviral silencing in C. parasitica activated by distinct viruses or transgene-derived double-stranded RNAs and provide insight into the potential for broad-spectrum virus control mediated by RNA silencing.

    DOI: 10.1073/pnas.1509151112

    Web of Science

    PubMed

    researchmap

  • Mycoreovirus genome rearrangements associated with RNA silencing deficiency 査読

    Ana Eusebio-Cope, Nobuhiro Suzuki

    NUCLEIC ACIDS RESEARCH   43 ( 7 )   3802 - 3813   2015年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Mycoreovirus 1 (MyRV1) has 11 double-stranded RNA genome segments (S1 to S11) and confers hypovirulence to the chestnut blight fungus, Cryphonectria parasitica. MyRV1 genome rearrangements are frequently generated by a multifunctional protein, p29, encoded by a positive-strand RNA virus, Cryphonectria hypovirus 1. One of its functional roles is RNA silencing suppression. Here, we explored a possible link between MyRV1 genome rearrangements and the host RNA silencing pathway using wild-type (WT) and mutant strains of both MyRV1 and the host fungus. Host strains included deletion mutants of RNA silencing components such as dicer-like (dcl) and argonaute-like (agl) genes, while virus strains included an S4 internal deletion mutant MyRV1/S4ss. Consequently, intragenic rearrangements with nearly complete duplication of the three largest segments, i.e. S1, S2 and S3, were observed even more frequently in the RNA silencing-deficient strains Delta dcl2 and Delta agl2 infected with MyRV1/S4ss, but not with any other viral/host strain combinations. An interesting difference was noted between genome rearrangement events in the two host strains, i.e. generation of the rearrangement required prolonged culture for Delta agl2 in comparison with Delta dcl2. These results suggest a role for RNA silencing that suppresses genome rearrangements of a dsRNA virus.

    DOI: 10.1093/nar/gkv239

    Web of Science

    PubMed

    researchmap

  • Exploration of the yadokari/yadonushi nature of YkV3 and RnMBV3 in the original host and a model filamentous fungus. 国際誌

    Yukiyo Sato, Sakae Hisano, Nobuhiro Suzuki

    Virus research   334   199155 - 199155   2023年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The yadokari/yadonushi nature is a recently discovered virus lifestyle; "yadokari" refers to the ability of capsidless positive-sense (+) RNA viruses (yadokariviruses) to utilize the capsids of phylogenetically distant double-stranded RNA (dsRNA) viruses possibly as the replication site, while "yadonushi" refers to the ability of dsRNA viruses to provide capsids to yadokariviruses. This virus-virus interaction, however, has been only studied with limited pathosystems. Here, we established a new study model with a capsidless (+)RNA yadokarivirus YkV3 (family Yadokariviridae) and its capsid donor RnMBV3 (family Megabirnaviridae) in the original host fungus Rosellinia necatrix and a model filamentous fungal host Cryphonectria parasitica. YkV3 has a simple genome structure with one open reading frame of 4305 nucleotides encoding a single polyprotein with an RNA-dependent RNA polymerase and a 2A-like self-cleavage peptide domain. Reverse genetics of YkV3 in R. necatrix showed that YkV3 tolerates a nucleotide substitution in the extreme 5'-terminus. The insertion of two termination codons immediately downstream of the 2A-like cleavage site abolished YkV3 viability, suggesting the importance of the C-terminal portion of the polyprotein of unknown function. Transfection of RnMBV3 and YkV3 into an RNA silencing-deficient mutant Δdcl2 of C. parasitica showed the replication competency of both viruses. Comparison between the wild-type and Δdcl2 strains of C. parasitica in virus accumulation suggested that RnMBV3 and YkV3 are susceptible to RNA silencing in C. parasitica. Taken together, we have established a platform to further explore the yadokari/yadonushi nature using genetically manipulable host fungal and virus strains.

    DOI: 10.1016/j.virusres.2023.199155

    PubMed

    researchmap

  • Continued mycovirus discovery expanding our understanding of virus lifestyles, symptom expression, and host defense. 国際誌

    Yukiyo Sato, Nobuhiro Suzuki

    Current opinion in microbiology   75   102337 - 102337   2023年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    High-throughput sequencing technologies have greatly expanded the RNA virome in general and have led to an exponential increase in new fungal viruses, also known as mycoviruses. Mycoviruses are omnipresent in fungi and usually induce symptomless infections. Some mycoviruses infecting fungi pathogenic to plants, insects, and mammals are known to modify host virulence positively and negatively and attract particular interests. In addition, fungal viruses continue to provide intriguing research materials and themes that lead to discoveries of peculiar viruses as infectious entities and insights into virus evolution and diversity. In this review, we outline the diversity and neolifestyle of recently discovered fungal RNA viruses, and phenotypic alterations induced by them. Furthermore, we discuss recent advances in research regarding the fungal antiviral defense and viral counterdefense, which are closely associated with host phenotype alterations. We hope that this article will enhance understanding of the interesting and growing fungal virology field.

    DOI: 10.1016/j.mib.2023.102337

    PubMed

    researchmap

  • Changes to virus taxonomy and the ICTV Statutes ratified by the International Committee on Taxonomy of Viruses (2023)

    Francisco Murilo Zerbini, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas‐Zerbini, Donald M. Dempsey, Bas E. Dutilh, María Laura García, R. Curtis Hendrickson, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lamber, Małgorzata Łobocka, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne‐Mieke Vandamme, Arvind Varsani

    168 ( 7 )   175   2023年6月

     詳細を見る

  • Sequence and phylogenetic analysis of a novel alphaendornavirus, the first virus described from the oomycete plant pathogen Phytophthora heveae

    Milica Raco, Thomas Jung, Marilia Horta Jung, Nguyen Minh Chi, Leticia Botella, Nobuhiro Suzuki

    Archives of Virology   168 ( 6 )   2023年5月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    Abstract

    Here, we report the discovery and complete genome sequence of a novel virus, designated as “Phytophthora heveae alphaendornavirus 1” (PhAEV1), from a single isolate of the plant pathogenic oomycete Phytophthora heveae (kingdom Stramenipila) isolated from a tropical evergreen lowland rainforest in northern Vietnam. PhAEV1 was detected by both cellulose affinity chromatography of dsRNA and high-throughput sequencing of total RNA, and its presence and sequence were confirmed by RT-PCR and Sanger sequencing. The PhAEV1 genome, 12,820 nucleotides (nt) in length, was predicted to encode a single large polyprotein with the catalytic core domain of viral (superfamily 1) RNA helicase (HEL, amino acid [aa] positions 1,287-1,531), glycosyltransferase (GT, aa positions ca. 2,800-3,125), and RNA-directed RNA polymerase (RdRp, aa positions 3,875-4,112). PhAEV1 is the most similar to Phytophthora cactorum alphaendornavirus 3, sharing 39.4% and 39.1% nt and aa sequence identity, respectively. In addition to the first 5′-terminal AUG codon, three additional in-frame methionine codons were found in close proximity (nt 14-16, 96-98, and 176-178), suggesting potential additional translation initiation sites. Conserved RdRp motifs (A-E) similar to those detected in related endornaviruses were identified in PhAEV1, as well as in several previously described alphaendornaviruses from other Phytophthora species in which these motifs had not been identified previously. Phylogenetic analysis showed that PhAEV1 clusters with members of the genus Alphaendornavirus in the family Endornaviridae and is basal to two other alphaendornaviruses described from another oomycete, Phytophthora cactorum. PhAEV1 is the first virus reported in P. heveae.

    DOI: 10.1007/s00705-023-05786-7

    researchmap

    その他リンク: https://link.springer.com/article/10.1007/s00705-023-05786-7/fulltext.html

  • Virus taxonomy and the role of the International Committee on Taxonomy of Viruses (ICTV)

    Stuart G. Siddell, Donald B. Smith, Evelien Adriaenssens, Poliane Alfenas-Zerbini, Bas E. Dutilh, Maria Laura Garcia, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Elliot J. Lefkowitz, Małgorzata Łobocka, Arcady R. Mushegian, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani, F. Murilo Zerbini

    Journal of General Virology   104 ( 5 )   2023年5月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology Society  

    The taxonomy of viruses is developed and overseen by the International Committee on Taxonomy of Viruses (ICTV), which scrutinizes, approves and ratifies taxonomic proposals, and maintains a list of virus taxa with approved names (https://ictv.global). The ICTV has approximately 180 members who vote by simple majority. Taxon-specific Study Groups established by the ICTV have a combined membership of over 600 scientists from the wider virology community; they provide comprehensive expertise across the range of known viruses and are major contributors to the creation and evaluation of taxonomic proposals. Proposals can be submitted by anyone and will be considered by the ICTV irrespective of Study Group support. Thus, virus taxonomy is developed from within the virology community and realized by a democratic decision-making process. The ICTV upholds the distinction between a virus or replicating genetic element as a physical entity and the taxon category to which it is assigned. This is reflected by the nomenclature of the virus species taxon, which is now mandated by the ICTV to be in a binomial format (genus + species epithet) and is typographically distinct from the names of viruses. Classification of viruses below the rank of species (such as, genotypes or strains) is not within the remit of the ICTV. This article, authored by the ICTV Executive Committee, explains the principles of virus taxonomy and the organization, function, processes and resources of the ICTV, with the aim of encouraging greater understanding and interaction among the wider virology community.

    DOI: 10.1099/jgv.0.001840

    researchmap

  • Structural features of T-DNA that induce transcriptional gene silencing during agroinfiltration

    Emi Iida, Kazunori Kuriyama, Midori Tabara, Atsushi Takeda, Nobuhiro Suzuki, Hiromitsu Moriyama, Toshiyuki Fukuhara

    2023年4月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Research Square Platform {LLC}  

    <jats:title>Abstract</jats:title>
    <jats:p><jats:italic>Agrobacterium tumefaciens</jats:italic> (<jats:italic>Rhizobium radiobacter</jats:italic>) is used for the transient expression of foreign genes by the agroinfiltration method, but the introduction of foreign genes often induces transcriptional and/or post-transcriptional gene silencing (TGS and/or PTGS). In this study, we characterized the structural features of T-DNA that induce TGS during agroinfiltration. When <jats:italic>A. tumefaciens</jats:italic>cells harboring an empty T-DNA plasmid containing the cauliflower mosaic virus (CaMV) 35S promoter were infiltrated into the leaves<jats:italic> </jats:italic>of <jats:italic>Nicotiana benthamiana</jats:italic> line 16c with a GFP gene over-expressed under the control of the same promoter, no small interfering RNAs (siRNAs) were derived from the GFP sequence. However, siRNAs derived from the CaMV 35S promoter were detected, indicating that TGS against the GFP gene was induced. When the GFP gene was inserted into the T-DNA plasmid, PTGS against the GFP gene was induced whereas TGS against the CaMV 35S promoter was suppressed. In other words, depending on the combination of promoter and coding sequences on T-DNA and the host nuclear genome, either or both TGS and/or PTGS could be induced by agroinfiltration. We also showed the importance of terminator sequences in T-DNA for gene silencing and the possible involvement of three siRNA-producing Dicers in the induction of TGS. These results are valuable for controlling gene expression by agroinfiltration.</jats:p>

    DOI: 10.21203/rs.3.rs-2796710/v1

    researchmap

  • Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan

    Hideki Kondo, Hitomi Sugahara, Miki Fujita, Kiwamu Hyodo, Ida Bagus Andika, Hiroshi Hisano, Nobuhiro Suzuki

    Pathogens   12 ( 3 )   358 - 358   2023年2月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    Many aphid-borne viruses are important pathogens that affect wheat crops worldwide. An aphid-transmitted closterovirus named wheat yellow leaf virus (WYLV) was found to have infected wheat plants in Japan in the 1970s; however, since then, its viral genome sequence and occurrence in the field have not been investigated. We observed yellowing leaves in the 2018/2019 winter wheat-growing season in an experimental field in Japan where WYLV was detected five decades ago. A virome analysis of those yellow leaf samples lead to the discovery of a closterovirus together with a luteovirus (barley yellow dwarf virus PAV variant IIIa). The complete genomic sequence of this closterovirus, named wheat closterovirus 1 isolate WL19a (WhCV1-WL19a), consisted of 15,452 nucleotides harboring nine open reading frames. Additionally, we identified another WhCV1 isolate, WL20, in a wheat sample from the winter wheat-growing season of 2019/2020. A transmission test indicated that WhCV1-WL20 was able to form typical filamentous particles and transmissible by oat bird-cherry aphid (Rhopalosiphum pad). Sequence and phylogenetic analyses showed that WhCV1 was distantly related to members of the genus Closterovirus (family Closteroviridae), suggesting that the virus represents a novel species in the genus. Furthermore, the characterization of WhCV1-WL19a-derived small RNAs using high-throughput sequencing revealed highly abundant 22-nt-class small RNAs potentially derived from the 3′-terminal end of the WhCV1 negative-strand genomic RNA, indicating that this terminal end of the WhCV1 genome is likely particularly targeted for the synthesis of viral small RNAs in wheat plants. Our results provide further knowledge on closterovirus diversity and pathogenicity and suggest that the impact of WhCV1 on wheat production warrants further investigations.

    DOI: 10.3390/pathogens12030358

    researchmap

  • Four principles to establish a universal virus taxonomy

    Peter Simmonds, Evelien M. Adriaenssens, F. Murilo Zerbini, Nicola G. A. Abrescia, Pakorn Aiewsakun, Poliane Alfenas-Zerbini, Yiming Bao, Jakub Barylski, Christian Drosten, Siobain Duffy, W. Paul Duprex, Bas E. Dutilh, Santiago F. Elena, Maria Laura García, Sandra Junglen, Aris Katzourakis, Eugene V. Koonin, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Elliot J. Lefkowitz, Małgorzata Łobocka, Cédric Lood, Jennifer Mahony, Jan P. Meier-Kolthoff, Arcady R. Mushegian, Hanna M. Oksanen, Minna M. Poranen, Alejandro Reyes-Muñoz, David L. Robertson, Simon Roux, Luisa Rubino, Sead Sabanadzovic, Stuart Siddell, Tim Skern, Donald B. Smith, Matthew B. Sullivan, Nobuhiro Suzuki, Dann Turner, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani, Nikos Vasilakis

    PLOS Biology   21 ( 2 )   e3001922 - e3001922   2023年2月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

    A universal taxonomy of viruses is essential for a comprehensive view of the virus world and for communicating the complicated evolutionary relationships among viruses. However, there are major differences in the conceptualisation and approaches to virus classification and nomenclature among virologists, clinicians, agronomists, and other interested parties. Here, we provide recommendations to guide the construction of a coherent and comprehensive virus taxonomy, based on expert scientific consensus. Firstly, assignments of viruses should be congruent with the best attainable reconstruction of their evolutionary histories, i.e., taxa should be monophyletic. This fundamental principle for classification of viruses is currently included in the International Committee on Taxonomy of Viruses (ICTV) code only for the rank of species. Secondly, phenotypic and ecological properties of viruses may inform, but not override, evolutionary relatedness in the placement of ranks. Thirdly, alternative classifications that consider phenotypic attributes, such as being vector-borne (e.g., “arboviruses”), infecting a certain type of host (e.g., “mycoviruses,” “bacteriophages”) or displaying specific pathogenicity (e.g., “human immunodeficiency viruses”), may serve important clinical and regulatory purposes but often create polyphyletic categories that do not reflect evolutionary relationships. Nevertheless, such classifications ought to be maintained if they serve the needs of specific communities or play a practical clinical or regulatory role. However, they should not be considered or called taxonomies. Finally, while an evolution-based framework enables viruses discovered by metagenomics to be incorporated into the ICTV taxonomy, there are essential requirements for quality control of the sequence data used for these assignments. Combined, these four principles will enable future development and expansion of virus taxonomy as the true evolutionary diversity of viruses becomes apparent.

    DOI: 10.1371/journal.pbio.3001922

    researchmap

  • ICTV Virus Taxonomy Profile: Hadakaviridae. 招待 査読

    Sato, Y, Turina, M, Chiba, S, Okada, R. A, Bhatti, M. F, Kotta-Loizou, I, Coutts, R. H. A, Kondo, H, Sabanadzovic, S, Suzuki, N, ICTV Repor, Consortium

    Journal of General Virology   104   001820   2023年2月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    researchmap

  • Capsid structure of a fungal dsRNA megabirnavirus reveals its previously unidentified surface architecture 査読

    Wang, H, Salaipeth, L, Miyazaki, N, Suzuki, N, Okamoto, K

    PLoS Pathogens   19   e1011162 - e1011162   2023年2月

     詳細を見る

    記述言語:英語  

    DOI: 10.1371/journal.ppat.1011162

    researchmap

  • ICTV Virus Taxonomy Profile: Yadokariviridae 2023

    Yukiyo Sato, Subha Das, Leonardo Velasco, Massimo Turina, Hideki Osaki, Ioly Kotta-Loizou, Robert H. A. Coutts, Hideki Kondo, Sead Sabanadzovic, Nobuhiro Suzuki

    Journal of General Virology   104 ( 1 )   2023年1月

     詳細を見る

    担当区分:責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology Society  

    The family Yadokariviridae, with the genera Alphayadokarivirus and Betayadokarivirus, includes capsidless non-segmented positive-sense (+) RNA viruses that hijack capsids from phylogenetically distant double-stranded RNA viruses. Yadokarivirids likely replicate inside the hijacked heterocapsids using their own RNA-directed RNA polymerase, mimicking dsRNA viruses despite their phylogenetic placement in a (+) RNA virus lineage. Yadokarivirids can have negative or positive impacts on their host fungi, through interactions with the capsid donor dsRNA viruses. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) report on the family Yadokariviridae, which is available at ictv.global/report/yadokariviridae.

    DOI: 10.1099/jgv.0.001826

    researchmap

  • The simplest RNA replicons, viroids: A tribute to Ricardo Flores 招待

    Vicente Pallas, Francesco Di Serio, Nobuhiro Suzuki

    323   198996 - 198996   2023年1月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.virusres.2022.198996

    researchmap

  • ICTV Virus Taxonomy Profile: Spinareoviridae 2022 査読

    Jelle Matthijnssens, Houssam Attoui, Krisztián Bányai, Corina P. D. Brussaard, Pranav Danthi, Mariana del Vas, Terence S. Dermody, Roy Duncan, Qín Fāng, Reimar Johne, Peter P. C. Mertens, Fauziah Mohd Jaafar, John T. Patton, Takahide Sasaya, Nobuhiro Suzuki, Taiyun Wei

    Journal of General Virology   103 ( 11 )   2022年11月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1099/jgv.0.001781

    researchmap

  • Recent changes to virus taxonomy ratified by the International Committee on Taxonomy of Viruses (2022) 査読

    Peter J. Walker, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Donald M. Dempsey, Bas E. Dutilh, María Laura García, R. Curtis Hendrickson, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Hanna M. Oksanen, Richard J. Orton, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani, Francisco Murilo Zerbini

    Archives of Virology   167 ( 11 )   2429 - 2440   2022年11月

     詳細を見る

  • Identification of novel totiviruses from the ascomycetous fungus Geotrichum candidum. 国際誌

    Haris Ahmed Khan, Hideki Kondo, Sabitree Shahi, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of virology   2022年10月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s00705-022-05611-7

    PubMed

    researchmap

  • ICTV Virus Taxonomy Profile: Sedoreoviridae 2022 査読

    Jelle Matthijnssens, Houssam Attoui, Krisztián Bányai, Corina P. D. Brussaard, Pranav Danthi, Mariana del Vas, Terence S. Dermody, Roy Duncan, Qín Fāng, Reimar Johne, Peter P. C. Mertens, Fauziah Mohd Jaafar, John T. Patton, Takahide Sasaya, Nobuhiro Suzuki, Taiyun Wei

    Journal of General Virology   103 ( 10 )   2022年10月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1099/jgv.0.001782

    researchmap

  • Three-Layered Complex Interactions among Capsidless (+)ssRNA Yadokariviruses, dsRNA Viruses, and a Fungus 査読

    Yukiyo Sato, Sakae Hisano, Carlos José López-Herrera, Hideki Kondo, Nobuhiro Suzuki

    mBio   2022年8月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1128/mbio.01685-22

    researchmap

  • A Transfectable Fusagravirus from a Japanese Strain of Cryphonectria carpinicola with Spherical Particles. 査読 国際誌

    Subha Das, Sakae Hisano, Ana Eusebio-Cope, Hideki Kondo, Nobuhiro Suzuki

    Viruses   14 ( 8 )   2022年8月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/v14081722

    PubMed

    researchmap

  • Mycovirus Hunting Revealed the Presence of Diverse Viruses in a Single Isolate of the Phytopathogenic Fungus Diplodia seriata From Pakistan 査読

    Haris Ahmed Khan, Paul Telengech, Hideki Kondo, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Frontiers in Cellular and Infection Microbiology   12   2022年6月

     詳細を見る

    担当区分:責任著者   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fcimb.2022.913619

    researchmap

  • Mycovirus Diversity and Evolution Revealed/Inferred from Recent Studies. 招待 国際誌

    Hideki Kondo, Leticia Botella, Nobuhiro Suzuki

    Annual review of phytopathology   2022年5月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1146/annurev-phyto-021621-122122

    PubMed

    researchmap

  • A novel deltapartitivirus from red clover. 査読 国際誌

    Paul Telengech, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki

    Archives of virology   167 ( 4 )   1201 - 1204   2022年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s00705-022-05372-3

    PubMed

    researchmap

  • Differentiating between viruses and virus species by writing their names correctly 査読

    Francisco Murilo Zerbini, Stuart G. Siddell, Arcady R. Mushegian, Peter J. Walker, Elliot J. Lefkowitz, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Bas E. Dutilh, María Laura García, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani

    Archives of Virology   167 ( 4 )   1231 - 1234   2022年4月

     詳細を見る

  • Plant viruses and viroids in Japan 招待 査読

    Shin-ichi Fuji, Tomofumi Mochizuki, Mitsuru Okuda, Shinya Tsuda, Satoshi Kagiwada, Ken-Taro Sekine, Masashi Ugaki, Keiko T. Natsuaki, Masamichi Isogai, Tetsuo Maoka, Minoru Takeshita, Nobuyuki Yoshikawa, Kazuyuki Mise, Takahide Sasaya, Hideki Kondo, Kenji Kubota, Yasuyuki Yamaji, Toru Iwanami, Kazusato Ohshima, Kappei Kobayashi, Tatsuji Hataya, Teruo Sano, Nobuhiro Suzuki

    88 ( 2 )   105 - 127   2022年3月

     詳細を見る

    担当区分:責任著者   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s10327-022-01051-y

    researchmap

    その他リンク: https://link.springer.com/article/10.1007/s10327-022-01051-y/fulltext.html

  • A novel victorivirus from the phytopathogenic fungus Neofusicoccum parvum 査読

    Haris Ahmed Khan, Yukiyo Sato, Hideki Kondo, Atif Jamal, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of Virology   2022年2月

     詳細を見る

  • A new tetra-segmented splipalmivirus with divided RdRP domains from Cryphonectria naterciae, a fungus found on chestnut and cork oak trees in Europe 査読

    Yukiyo Sato, Sabitree Shahi, Paul Telengech, Sakae Hisano, Carolina Cornejo, Daniel Rigling, Hideki Kondo, Nobuhiro Suzuki

    Virus Research   307   198606 - 198606   2022年1月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.virusres.2021.198606

    Scopus

    researchmap

  • Assessment of mycoviral diversity in Pakistani fungal isolates revealed infection by 11 novel viruses of a single strain of Fusarium mangiferae isolate SP1. 国際誌

    Haris Ahmed Khan, Wajeeha Shamsi, Atif Jamal, Memoona Javaied, Mashal Sadiq, Tehsin Fatma, Aqeel Ahmed, Maleeha Arshad, Mubashra Waseem, Samra Babar, Midhat Mustafa Dogar, Nasar Virk, Hussnain Ahmed Janjua, Hideki Kondo, Nobuhiro Suzuki, Muhammad Faraz Bhatti

    The Journal of general virology   102 ( 12 )   2021年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    An extensive screening survey was conducted on Pakistani filamentous fungal isolates for the identification of viral infections. A total of 396 fungal samples were screened, of which 36 isolates were found double-stranded (ds) RNA positive with an overall frequency of 9% when analysed by a classical dsRNA isolation method. One of 36 dsRNA-positive strains, strain SP1 of a plant pathogenic fungus Fusarium mangiferae, was subjected to virome analysis. Next-generation sequencing and subsequent completion of the entire genome sequencing by a classical Sanger sequencing method showed the SP1 strain to be co-infected by 11 distinct viruses, at least seven of which should be described as new taxa at the species level according to the ICTV (International Committee on Taxonomy of Viruses) species demarcation criteria. The newly identified F. mangiferae viruses (FmVs) include two partitivirids, one betapartitivirus (FmPV1) and one gammapartitivirus (FmPV2); six mitovirids, three unuamitovirus (FmMV2, FmMV4, FmMV6), one duamitovirus (FmMV5), and two unclassified mitovirids (FmMV1, FmMV3); and three botourmiavirids, two magoulivirus (FmBOV1, FmBOV3) and one scleroulivirus (FmBOV2). The number of coinfecting viruses is among the largest ones of fungal coinfections. Their molecular features are thoroughly described here. This represents the first large virus survey in the Indian sub-continent.

    DOI: 10.1099/jgv.0.001690

    PubMed

    researchmap

  • Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand 査読

    Sokty Neang, Santiti Bincader, Sansern Rangsuwan, Pisut Keawmanee, Soriya Rin, Lakha Salaipeth, Subha Das, Hideki Kondo, Nobuhiro Suzuki, Ikuo Sato, Daigo Takemoto, Chainarong Rattanakreetakul, Ratiya Pongpisutta, Masao Arakawa, Sotaro Chiba

    Viruses   13 ( 11 )   2269 - 2269   2021年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    Partitiviruses are one of the most prevalent double-stranded RNA viruses that have been identified mostly in filamentous fungi and plants. Partitiviruses generally infect host fungi asymptomatically but infrequently exert significant effect(s) on morphology and virulence, thus being considered a potential source of biological control agents against pathogenic fungi. In this study, we performed a screening for mycoviruses of a collection of Thai isolates of rice fungal pathogen Rhizoctonia oryzae-sativae, a causal agent of rice aggregated sheath spot disease. As a result, 36% of tested isolates carried potentially viral double-stranded RNAs with sizes ranging from 2 to 3 kbp. By conventional cDNA library construction and RNA-seq, we determined six new alphapartitiviruses that infected three isolates: tentatively named Rhizoctonia oryzae-sativae partitivirus 1 to 6 (RosPV1-6). Furthermore, RT-PCR detection of each virus revealed their omnipresent nature in different R. oryzae-sativae isolates. Although virus-curing of basidiomycetous fungi is generally difficult, our repeated attempts successfully obtained virus-free (for RosPV1, RosPV2, and uncharacterized partitiviruses), isogenic strain of R. oryzae-sativae TSS190442. The virus-cured strain showed slightly faster colony growth on the synthetic media and severe symptom development on the rice sheath compared to its virus-infected counterpart. Overall, this study shed light on the distribution of partitiviruses in R. oryzae-sativae in a paddy environment and exemplified a virus-curing protocol that may be applicable for other basidiomycetous fungi.

    DOI: 10.3390/v13112269

    Scopus

    researchmap

  • A new double-stranded rna mycovirus in cryphonectria naterciae is able to cross the species barrier and is deleterious to a new host 査読

    Carolina Cornejo, Sakae Hisano, Helena Bragança, Nobuhiro Suzuki, Daniel Rigling

    Journal of Fungi   7 ( 10 )   861   2021年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cryphonectria is a fungal genus associated with economically significant disease of trees. Herein we characterized a novel double-stranded RNA virus from the fungal species Cryphonectria naterciae, a species unexplored as a virus host. De novo assembly of RNA-seq data and Sanger sequencing of RACE (rapid amplification of cDNA ends) clones gave the complete, non-segmented genome (10,164 bp) of the virus termed Cryphonectria naterciae fusagravirus (CnFGV1) that was phylogenetically placed within the previously proposed viral family Fusagraviridae. Of 31 fieldcollected strains of C. naterciae, 40% tested CnFGV1-positive. Cocultivation resulted in within-species transmission of CnFGV1 to virus-free strains of C. naterciae. Comparison of the mycelium phenotype and the growth rate of CnFGV1-infected and virus-free isogenic strains revealed frequent sectoring and growth reduction in C. naterciae upon virus infection. Co-culturing also led to cross-species transmission of CnFGV1 to Cryphonectria carpinicola and Cryphonectria radicalis, but not to Cryphonectria parasitica. The virus-infected C. naterciae and the experimentally infected Cryphonectria spp. readily transmitted CnFGV1 through asexual spores to the next generation. CnFGV1 strongly reduced conidiation and in some cases vegetative growth of C. carpinicola, which is involved in the European hornbeam disease. This study is the first report of a fusagravirus in the family Cryphonectriaceae and lays the groundwork for assessing a hypovirulence effect of CnFGV1 against the hornbeam decline in Europe.

    DOI: 10.3390/jof7100861

    Scopus

    researchmap

  • Changes to virus taxonomy and to the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2021) 査読

    Peter J. Walker, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Andrew J. Davison, Donald M. Dempsey, Bas E. Dutilh, María Laura García, Balázs Harrach, Robert L. Harrison, R. Curtis Hendrickson, Sandra Junglen, Nick J. Knowles, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Max L. Nibert, Hanna M. Oksanen, Richard J. Orton, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Dooerslaer, Anne-Mieke Vandamme, Arvind Varsani, Francisco Murilo Zerbini

    Archives of Virology   166 ( 9 )   2633 - 2648   2021年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s00705-021-05156-1

    Scopus

    PubMed

    researchmap

    その他リンク: https://link.springer.com/article/10.1007/s00705-021-05156-1/fulltext.html

  • A second capsidless hadakavirus strain with 10 positive-sense single-stranded RNA genomic segments from Fusarium nygamai 査読

    Haris Ahmed Khan, Yukiyo Sato, Hideki Kondo, Atif Jamal, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of Virology   166   2711 - 2722   2021年7月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s00705-021-05176-x

    researchmap

    その他リンク: https://link.springer.com/article/10.1007/s00705-021-05176-x/fulltext.html

  • In-tree behavior of diverse viruses harbored in the chestnut blight fungus, Cryphonectria parasitica 査読

    Nobuhiro Suzuki, Carolina Cornejo, Annisa Aulia, Sabitree Shahi, Bradley I. Hillman, Daniel Rigling

    Journal of Virology   95 ( 6 )   e01962-20   2021年3月

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The ascomycete Cryphonectria parasitica causes destructive chestnut blight. Biological control of the fungus by virus infection (hypovirulence) has been shown to be an effective control strategy against chestnut blight in Europe. To provide biocontrol effects, viruses must be able to induce hypovirulence and spread efficiently in chestnut trees. Field studies using living trees to date have focused on a selected family of viruses called hypoviruses, especially prototypic hypovirus CHV1, but there are now known to be many other viruses that infect C. parasitica. Here, we tested seven different viruses for their hypovirulence induction, biocontrol potential, and transmission properties between two vegetatively compatible but molecularly distinguishable fungal strains in trees. The test included cytosolically and mitochondrially replicating viruses with positive-sense single-stranded RNA or double-stranded RNA genomes. The seven viruses showed different in planta behaviors and were classified into four groups. Group I, including CHV1, had great biocontrol potential and could protect trees by efficiently spreading and converting virulent to hypovirulent cankers in the trees. Group II could induce high levels of hypovirulence but showed much smaller biocontrol potential, likely because of inefficient virus transmission. Group III showed poor performance in hypovirulence induction and biocontrol, while efficiently being transmitted in the infected trees. Group IV could induce hypovirulence and spread efficiently but showed poor biocontrol potential. Nuclear and mitochondrial genotyping of fungal isolates obtained from the treated cankers confirmed virus transmission between the two fungal strains in most isolates. These results are discussed in view of dynamic interactions in the tripartite pathosystem. IMPORTANCE The ascomycete Cryphonectria parasitica causes destructive chestnut blight, which is controllable by hypovirulence-conferring viruses infecting the fungus. The tripartite chestnut/C. parasitica/virus pathosystem involves the dynamic interactions of their genetic elements, i.e., virus transmission and lateral transfer of nuclear and mitochondrial genomes between fungal strains via anastomosis occurring in trees. Here, we tested diverse RNA viruses for their hypovirulence induction, biocontrol potential, and transmission properties between two vegetatively compatible but molecularly distinguishable fungal strains in live chestnut trees. The tested viruses, which are different in genome type (single-stranded or double-stranded RNA) and organization, replication site (cytosol or mitochondria), virus form (encapsidated or capsidless) and/or symptomatology, have been unexplored in the aforementioned aspects under controlled conditions. This study showed intriguing different in-tree behaviors of the seven viruses and suggested that to exert significant biocontrol effects, viruses must be able to induce hypovirulence and spread efficiently in the fungus infecting the chestnut trees.

    DOI: 10.1128/JVI.01962-20

    Scopus

    PubMed

    researchmap

  • Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range 査読

    Sabitree Shahi, Sotaro Chiba, Hideki Kondo, Nobuhiro Suzuki

    Virology   554   55 - 65   2021年2月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cryphonectria nitschkei chrysovirus 1 (CnCV1), was described earlier from an ascomycetous fungus, Cryphonectria nitschkei strain OB5/11, collected in Japan; its partial sequence was reported a decade ago. Complete sequencing of the four genomic dsRNA segments revealed molecular features similar to but distinct from previously reported members of the family Chrysoviridae. Unique features include the presence of a mini-cistron preceding the major large open reading frame in each genomic segment. Common features include the presence of CAA repeats in the 5′-untranslated regions and conserved terminal sequences. CnCV1-OB5/11 could be laterally transferred to C. nitschkei and its relatives C. radicalis and C. naterciae via coculturing, virion transfection and protoplast fusion, but not to fungal species other than the three species mentioned above, even within the genus Cryphonectria, suggesting a very narrow host range. Phenotypic comparison of a few sets of CnCV1-infected and -free isogenic strains showed symptomless infection in new hosts.

    DOI: 10.1016/j.virol.2020.11.011

    Scopus

    PubMed

    researchmap

  • Identification of an RNA silencing suppressor encoded by a symptomless fungal hypovirus, Cryphonectria hypovirus 4 査読

    Annisa Aulia, Kiwamu Hyodo, Sakae Hisano, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

    Biology   10 ( 2 )   1 - 16   2021年2月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Previously, we have reported the ability of a symptomless hypovirus Cryphonectria hypovirus 4 (CHV4) of the chestnut blight fungus to facilitate stable infection by a co-infecting mycoreovirus 2 (MyRV2)—likely through the inhibitory effect of CHV4 on RNA silencing (Aulia et al., Virology, 2019). In this study, the N-terminal portion of the CHV4 polyprotein, termed p24, is identified as an autocatalytic protease capable of suppressing host antiviral RNA silencing. Using a bacterial expression system, CHV4 p24 is shown to cleave autocatalytically at the di-glycine peptide (Gly214-Gly215) of the polyprotein through its protease activity. Transgenic expression of CHV4 p24 in Cryphonectria parasitica suppresses the induction of one of the key genes of the antiviral RNA silencing, dicer-like 2, and stabilizes the infection of RNA silencing-susceptible virus MyRV2. This study shows functional similarity between CHV4 p24 and its homolog p29, encoded by the symptomatic prototype hypovirus CHV1.

    DOI: 10.3390/biology10020100

    Scopus

    researchmap

  • Biochemical characterization of the dicing activity of Dicer-like 2 in the model filamentous fungus Neurospora crassa 査読

    Midori Tabara, Hisashi Koiwa, Nobuhiro Suzuki, Toshiyuki Fukuhara

    Fungal Genetics and Biology   146   103488   2021年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Dicing of double-stranded RNA (dsRNA) into small RNA is an essential process to trigger transcriptional and post-transcriptional gene silencing. Using cell-free extracts of the model filamentous fungus Neurospora crassa, we successfully detected the dicing activity of one of two N. crassa Dicers NcDCL2. The predominant 23-nucleotide (nt) cleavage product was always detected from 30-nt to 130-nt dsRNA substrates, and additional products of approximately 18 to 28 nt were occasionally produced. The enzymatic properties of NcDCL2 are different from those of insect and plant small interfering RNA (siRNA)-producing Dicers, Drosophila melanogaster Dicer-2 and Arabidopsis thaliana DCL3 and DCL4 (AtDCL3 and AtDCL4). Whereas AtDCL3 and AtDCL4 preferentially cleave short and long dsRNAs, respectively, NcDCL2 cleaved both short and long dsRNAs. These results suggest that N. crassa has a single siRNA-producing Dicer NcDCL2, which is a prototype of plant siRNA-producing Dicers with distinct functions in diverse RNA silencing pathways. The dicing assay reported here is convenient to detect and biochemically characterize the dicing activities of both plant and fungal Dicers, and is likely applicable to other organisms.

    DOI: 10.1016/j.fgb.2020.103488

    Scopus

    PubMed

    researchmap

  • A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix 査読

    Juan M. Arjona-López, Paul Telengech, Nobuhiro Suzuki, Carlos J. López-Herrera

    Fungal Biology   125 ( 1 )   69 - 76   2021年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Two isolates of Rosellinia necatrix (Rn118-8 and Rn480) have previously obtained from diseased avocado trees in commercial orchards of the coastal area in southern Spain. Rn118-8 and Rn480 have weak virulence on avocado plants, and are infected by R. necatrix hypovirus 2 (RnHV2). In this work, the possible biological effects of the hypovirus on R. necatrix were tested. First, RnHV2 was transmitted from each of Rn118-8 and Rn480 to a highly virulent, RnHV2-free isolate of R. necatrix (Rn400) through hyphal anastomosis, using zinc compounds which attenuate the mycelial incompatibility reactions and allow for horizontal virus transfer between vegetatively incompatible fungal strains. Next, we carried out an analysis of growth rate in vitro and a virulence test of these newly infected strains in avocado plants. We obtained five strains of Rn400 infected by RnHV2 after horizontal transmission, and showed some of them to have lower colony growth in vitro and lower virulence on avocado plants compared with virus-free Rn400. These results suggest that R. necatrix isolates infected by RnHV2 could be used as novel virocontrol agents to combat avocado white root rot.

    DOI: 10.1016/j.funbio.2020.10.007

    Scopus

    PubMed

    researchmap

  • Identification of a Novel Quinvirus in the Family Betaflexiviridae That Infects Winter Wheat. 査読 国際誌

    Hideki Kondo, Naoto Yoshida, Miki Fujita, Kazuyuki Maruyama, Kiwamu Hyodo, Hiroshi Hisano, Tetsuo Tamada, Ida Bagus Andika, Nobuhiro Suzuki

    Frontiers in microbiology   12   715545   2021年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Yellow mosaic disease in winter wheat is usually attributed to the infection by bymoviruses or furoviruses; however, there is still limited information on whether other viral agents are also associated with this disease. To investigate the wheat viromes associated with yellow mosaic disease, we carried out de novo RNA sequencing (RNA-seq) analyses of symptomatic and asymptomatic wheat-leaf samples obtained from a field in Hokkaido, Japan, in 2018 and 2019. The analyses revealed the infection by a novel betaflexivirus, which tentatively named wheat virus Q (WVQ), together with wheat yellow mosaic virus (WYMV, a bymovirus) and northern cereal mosaic virus (a cytorhabdovirus). Basic local alignment search tool (BLAST) analyses showed that the WVQ strains (of which there are at least three) were related to the members of the genus Foveavirus in the subfamily Quinvirinae (family Betaflexiviridae). In the phylogenetic tree, they form a clade distant from that of the foveaviruses, suggesting that WVQ is a member of a novel genus in the Quinvirinae. Laboratory tests confirmed that WVQ, like WYMV, is potentially transmitted through the soil to wheat plants. WVQ was also found to infect rye plants grown in the same field. Moreover, WVQ-derived small interfering RNAs accumulated in the infected wheat plants, indicating that WVQ infection induces antiviral RNA silencing responses. Given its common coexistence with WYMV, the impact of WVQ infection on yellow mosaic disease in the field warrants detailed investigation.

    DOI: 10.3389/fmicb.2021.715545

    PubMed

    researchmap

  • Molecular Characterization of a Novel Polymycovirus From Penicillium janthinellum With a Focus on Its Genome-Associated PASrp 査読

    Yukiyo Sato, Atif Jamal, Hideki Kondo, Nobuhiro Suzuki

    Frontiers in Microbiology   11   e0045-20   2020年10月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The genus Polymycovirus of the family Polymycoviridae accommodates fungal RNA viruses with different genomic segment numbers (four, five, or eight). It is suggested that four members form no true capsids and one forms filamentous virus particles enclosing double-stranded RNA (dsRNA). In both cases, viral dsRNA is associated with a viral protein termed “proline-alanine-serine-rich protein” (PASrp). These forms are assumed to be the infectious entity. However, the detailed molecular characteristics of PASrps remain unclear. Here, we identified a novel five-segmented polymycovirus, Penicillium janthinellum polymycovirus 1 (PjPmV1), and characterized its purified fraction form in detail. The PjPmV1 had five dsRNA segments associated with PASrp. Density gradient ultracentrifugation of the PASrp-associated PjPmV1 dsRNA revealed its uneven structure and a broad fractionation profile distinct from that of typical encapsidated viruses. Moreover, PjPmV1-PASrp interacted in vitro with various nucleic acids in a sequence-non-specific manner. These PjPmV1 features are discussed in view of the diversification of genomic segment numbers of the genus Polymycovirus.

    DOI: 10.3389/fmicb.2020.592789

    Scopus

    researchmap

  • Coinfection of Rosellinia necatrix by a partitivirus and a virga-like virus is associated with hypovirulence 査読

    Juan M. Arjona-López, Paul Telengech, Nobuhiro Suzuki, Carlos J. López-Herrera

    European Journal of Plant Pathology   158 ( 1 )   111 - 119   2020年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Many Mediteranean isolates of Rosellinia necatrix, causing root rot in avocado, have previously been screened for viruses. Among them is isolate Rn459 that has been shown to be infected by at least three viruses such as Rosellinia necatrix partitivirus 10 (RnPV10), Rosellinia necatrix hypovirus 2 (RnHV2), Rosellinia necatrix fusagravirus 1 (RnFGV1), and Rosellinia necatrix virga-like virus (RnVLV). Here, we attempted to eliminate the viruses by hyphal tip cultures to examine their effect on colony growth in vitro and virulence on avocado plants. The obtained fungal strain termed, Rn459_PV10F/VLVF, which was confirmed to be cured of RnPV10 and RnVLV, but still retaining RnHV2, manifested a phenotype different from the original Rn459. Colony growth comparison showed that Rn459_PV10F/VLVF grew faster than the original Rn459 isolate and the virulence on avocado plants of this Rn459_PV10F/VLVF strain was higher than the original Rn459 strain. These combined results suggest that RnPV10 and RnVLV, alone or together, contribute to confer hypovirulence on the R. necatrix isolates.

    DOI: 10.1007/s10658-020-02058-x

    Scopus

    researchmap

  • Dicer monitoring in a model filamentous fungus host, Cryphonectria parasitica 査読

    Annisa Aulia, Midori Tabara, Paul Telengech, Toshiyuki Fukuhara, Nobuhiro Suzuki

    Current Research in Virological Science   100001 - 100001   2020年7月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier {BV}  

    DOI: 10.1016/j.crviro.2020.100001

    researchmap

  • Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix 査読

    Paul Telengech, Sakae Hisano, Cyrus Mugambi, Kiwamu Hyodo, Juan Manuel Arjona-López, Carlos José López-Herrera, Satoko Kanematsu, Hideki Kondo, Nobuhiro Suzuki

    Frontiers in Microbiology   11   1064   2020年6月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report the characterization of a total of 20 partitiviruses from 16 R. necatrix strains belonging to 15 new species, for which “Rosellinia necatrix partitivirus 11–Rosellinia necatrix partitivirus 25” were proposed, and 5 previously reported species. The newly identified partitiviruses have been taxonomically placed in two genera, Alphapartitivirus, and Betapartitivirus. Some partitiviruses were transfected into reference strains of the natural host, R. necatrix, and an experimental host, Cryphonectria parasitica, using purified virions. A comparative analysis of resultant transfectants revealed interesting differences and similarities between the RNA accumulation and symptom induction patterns of R. necatrix and C. parasitica. Other interesting findings include the identification of a probable reassortment event and a quintuple partitivirus infection of a single fungal strain. These combined results provide a foundation for further studies aimed at elucidating mechanisms that underly the differences observed.

    DOI: 10.3389/fmicb.2020.01064

    Scopus

    researchmap

  • Virome Analysis of Aphid Populations That Infest the Barley Field: The Discovery of Two Novel Groups of Nege/Kita-Like Viruses and Other Novel RNA Viruses 査読 国際誌

    Hideki Kondo, Miki Fujita, Hiroshi Hisano, Kiwamu Hyodo, Ida Bagus Andika, Nobuhiro Suzuki

    Frontiers in Microbiology   11   509   2020年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aphids (order Hemiptera) are important insect pests of crops and are also vectors of many plant viruses. However, little is known about aphid-infecting viruses, particularly their diversity and relationship to plant viruses. To investigate the aphid viromes, we performed deep sequencing analyses of the aphid transcriptomes from infested barley plants in a field in Japan. We discovered virus-like sequences related to nege/kita-, flavi-, tombus-, phenui-, mononega-, narna-, chryso-, partiti-, and luteoviruses. Using RT-PCR and sequence analyses, we determined almost complete sequences of seven nege/kitavirus-like virus genomes; one of which was a variant of the Wuhan house centipede virus (WHCV-1). The other six seem to belong to four novel viruses distantly related to Wuhan insect virus 9 (WhIV-9) or Hubei nege-like virus 4 (HVLV-4). We designated the four viruses as barley aphid RNA virus 1 to 4 (BARV-1 to -4). Moreover, some nege/kitavirus-like sequences were found by searches on the transcriptome shotgun assembly (TSA) libraries of arthropods and plants. Phylogenetic analyses showed that BARV-1 forms a clade with WHCV-1 and HVLV-4, whereas BARV-2 to -4 clustered with WhIV-9 and an aphid virus, Aphis glycines virus 3. Both virus groups (tentatively designated as Centivirus and Aphiglyvirus, respectively), together with arthropod virus-like TSAs, fill the phylogenetic gaps between the negeviruses and kitaviruses lineages. We also characterized the flavi/jingmen-like and tombus-like virus sequences as well as other RNA viruses, including six putative novel viruses, designated as barley aphid RNA viruses 5 to 10. Interestingly, we also discovered that some aphid-associated viruses, including nege/kita-like viruses, were present in different aphid species, raising a speculation that these viruses might be distributed across different aphid species with plants being the reservoirs. This study provides novel information on the diversity and spread of nege/kitavirus-related viruses and other RNA viruses that are associated with aphids.

    DOI: 10.3389/fmicb.2020.00509

    Scopus

    PubMed

    researchmap

  • Editorial: Frontiers in Fungal Virus Research 査読

    Liying Sun, Nobuhiro Suzuki, Daohong Jiang, Massimo Turina, Jiatao Xie

    Frontiers in Cellular and Infection Microbiology   9   456   2020年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fcimb.2019.00456

    Scopus

    PubMed

    researchmap

  • Structure and assembly of double-stranded RNA mycoviruses 査読

    Carlos P. Mata, Javier M. Rodríguez, Nobuhiro Suzuki, José R. Castón

    Advances in Virus Research   108   213 - 247   2020年1月

     詳細を見る

    記述言語:英語   掲載種別:論文集(書籍)内論文  

    Mycoviruses are a diverse group that includes ssRNA, dsRNA, and ssDNA viruses, with or without a protein capsid, as well as with a complex envelope. Most mycoviruses are transmitted by cytoplasmic interchange and are thought to lack an extracellular phase in their infection cycle. Structural analysis has focused on dsRNA mycoviruses, which usually package their genome in a 120-subunit T = 1 icosahedral capsid, with a capsid protein (CP) dimer as the asymmetric unit. The atomic structure is available for four dsRNA mycovirus from different families: Saccharomyces cerevisiae virus L-A (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). Their capsids show structural variations of the same framework, with asymmetric or symmetric CP dimers respectively for ScV-L-A and PsV-F, dimers of similar domains of a single CP for PcV, or of two different proteins for RnQV1. The CP dimer is the building block, and assembly proceeds through dimers of dimers or pentamers of dimers, in which the genome is packed as ssRNA by interaction with CP and/or viral polymerase. These capsids remain structurally undisturbed throughout the viral cycle. The T = 1 capsid participates in RNA synthesis, organizing the viral polymerase (1–2 copies) and a single loosely packaged genome segment. It also acts as a molecular sieve, to allow the passage of viral transcripts and nucleotides, but to prevent triggering of host defense mechanisms. Due to the close mycovirus-host relationship, CP evolved to allocate peptide insertions with enzyme activity, as reflected in a rough outer capsid surface.

    DOI: 10.1016/bs.aivir.2020.08.001

    Scopus

    PubMed

    researchmap

  • Neo-virology: The raison d'etre of viruses 査読 国際誌

    Tokiko Watanabe, Nobuhiro Suzuki, Keizo Tomonaga, Hirofumi Sawa, Yoshiharu Matsuura, Yasushi Kawaguchi, Hideki Takahashi, Keizo Nagasaki, Yoshihiro Kawaoka

    Virus Research   274   197751 - 197751   2019年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Given that approximately 10 virus particles exist on Earth and all of them are parasitic in living organisms, it is not hard to imagine how virus infection might affect the physiology of hosts and their ecosystems. However, traditional virology research tends to focus on viral pathogenicity or the individual pathogenic viruses; hence, the significance of viruses and viral-mediated processes in the global ecosystem has been poorly understood. To identify the previously unrecognized “raison d'etre of viruses” in nature, we established a research community, designated as the ‘Neo-virology’ consortium. In this consortium, we define a virus as a component of the global ecosystem and our aim is to elucidate its key roles in host organisms, that is, the intra-host ecosystem. 31

    DOI: 10.1016/j.virusres.2019.197751

    Web of Science

    Scopus

    PubMed

    researchmap

  • ICTV Virus Taxonomy Profile: Megabirnaviridae. 査読 国際誌

    Sato, Y, Miyazaki, N, Kanematsu, S, Ghabrial, S. A, Hillman, B. I, Suzuki, N, ICTV Report, Consortium

    Journal of General Virology   100 ( 9 )   269 - 1270   2019年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1099/jgv.0.001297

    Web of Science

    PubMed

    researchmap

  • Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae 査読

    Shuhei Ohkita, Yui Lee, Quyet Nguyen, Kenichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

    Virology   534   25 - 35   2019年8月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5’ terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.

    DOI: 10.1016/j.virol.2019.05.015

    Web of Science

    Scopus

    PubMed

    researchmap

  • Isolation and characterization of a novel mycovirus infecting an edible mushroom, Grifola frondosa 査読

    Akiko Komatsu, Hideki Kondo, Masayuki Sato, Atsushi Kurahashi, Kozo Nishibori, Nobuhiro Suzuki, Fumihiro Fujimori

    Mycoscience   60 ( 4 )   211 - 220   2019年7月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    Grifola frondosa (Maitake mushroom) is an important cultivated mushroom due to its medicinal and nutrient values. In this study, we isolated and characterized a novel partitivirus (named Grifola frondosa partitivirus 1, GfPV1) infecting a standard G. frondosa strain Gf-N2. This virus has a two-segmented dsRNA genome (dsRNA1 and dsRNA2) with nucleotide lengths of 2.3 and 2.2 kbp, respectively. The coding strand of dsRNA1 and dsRNA2 segments carries single open reading frame encoding RNA-dependent RNA polymerase (RdRp) and a coat protein (CP), respectively. BLAST searches and phylogenetic analyses showed that GfPV1 is most closely related to a betapartitivirus, Lentinula edodes partitivirus 1 (RdRp <70% and CP <60% amino acid sequence identities), but the sequence divergence suggests that GfPV1 is classifiable as a new member of the genus Betapartitivirus, family Partitiviridae. The presence of GfPV1 does not affect colony morphology and fruiting body development of G. frondosa. This is the first report investigating the effects of a mycovirus infection on the colony morphology and fruiting body development of G. frondosa. Interestingly, GfPV1 accumulations markedly decreased along with the fruiting body maturation stages, suggesting the inhibition of virus multiplication during sexual phase of the G. frondosa life cycle.

    DOI: 10.1016/j.myc.2019.01.005

    Web of Science

    Scopus

    researchmap

  • A symptomless hypovirus, CHV4, facilitates stable infection of the chestnut blight fungus by a coinfecting reovirus likely through suppression of antiviral RNA silencing 査読

    Annisa Aulia, Ida Bagus Andika, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

    Virology   533   99 - 107   2019年7月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier {BV}  

    Field-collected US strain C18 of Cryphonectria parasitica, the chestnut blight fungus, was earlier reported to be infected by a double-stranded RNA virus, mycoreovirus 2 (MyRV2). Next-generation sequencing has revealed co-infection of C18 by a positive-strand RNA virus, hypovirus 4 (CHV4). The current molecular and genetic analyses showed interesting commensal interactions between the two viruses. CHV4 facilitated the stable infection and enhanced vertical transmission of MyRV2, which was readily lost during subculturing and showed reduced vertical transmission in single infections. Deletion of a key antiviral RNA silencing gene, dcl2, in isolate C18 increased stability of MyRV2 in single infections. The ability of CHV4 to facilitate stable infection with MyRV2 appears to be associated with the inhibitory effect of CHV4 on RNA silencing via compromising the induction of transcriptional up-regulation of dcl2. These results suggest that natural infection of isolate C18 by MyRV2 in the field was facilitated by CHV4 co-infection.

    DOI: 10.1016/j.virol.2019.05.004

    Web of Science

    Scopus

    PubMed

    researchmap

  • Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes) 査読

    Yu Hsin Lin, Miki Fujita, Sotaro Chiba, Kiwamu Hyodo, Ida Bagus Andika, Nobuhiro Suzuki, Hideki Kondo

    Virology   533   125 - 136   2019年7月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    There is still limited information on the diversity of (−)ssRNA viruses that infect fungi. Here, we have discovered two novel (−)ssRNA mycoviruses in the shiitake mushroom (Lentinula edodes). The first virus has a monopartite RNA genome and relates to that of mymonaviruses (Mononegavirales), especially to Hubei rhabdo-like virus 4 from arthropods and thus designated as Lentinula edodes negative-strand RNA virus 1. The second virus has a putative bipartite RNA genome and is related to the recently discovered bipartite or tripartite phenui-like viruses (Bunyavirales) associated with plants and ticks, and designated as Lentinula edodes negative-strand RNA virus 2 (LeNSRV2). LeNSRV2 is likely the first segmented (−)ssRNA virus known to infect fungi. Its smaller RNA segment encodes a putative nucleocapsid and a plant MP-like protein using a potential ambisense coding strategy. These findings enhance our understanding of the diversity, evolution and spread of (−)ssRNA viruses in fungi.

    DOI: 10.1016/j.virol.2019.05.008

    Web of Science

    Scopus

    PubMed

    researchmap

  • Novel victorivirus from a pakistani isolate of alternaria alternata lacking a typical translational stop/restart sequence signature 査読

    Atif Jamal, Yukiyo Sato, Sabitree Shahi, Wajeeha Shamsi, Hideki Kondo, Nobuhiro Suzuki

    Viruses   11 ( 6 )   577   2019年6月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI} {AG  

    The family Totiviridae currently contains five genera Totivirus, Victorivirus, Leishmavirus, Trichomonasvirus, and Giardiavirus. Members in this family generally have a set of two-open reading frame (ORF) elements in their genome with the 5’-proximalORF (ORF1) encoding a capsid protein (CP) and the 3’-proximal one (ORF2) for RNA-dependent RNA polymerase (RdRp). How the downstream open reading frames (ORFs) are expressed is genus-specific. All victoriviruses characterized thus far appear to use the stop/restart translation mechanism, allowing for the expression of two separate protein products from bicitronic genome-sized viral mRNA, while the totiviruses use a -1 ribosomal frame-shifting that leads to a fusion product of CP and RdRp. We report the biological and molecular characterization of a novel victorivirus termed Alternaria alternata victorivirus 1 (AalVV1) isolated from Alternaria alternata in Pakistan. The phylogenetic and molecular analyses showed AalVV1 to be distinct from previously reported victoriviruses. AalVV1 appears to have a sequence signature required for the -1 frame-shifting at the ORF1/2 junction region, rather than a stop/restart key mediator. By contrast, SDS–polyacrylamide gel electrophoresis and peptide mass fingerprinting analyses of purified virion preparations suggested the expression of two protein products, not a CP-RdRp fusion product. How these proteins are expressed is discussed in this study. Possible effects of infection by this virus were tested in two fungal species: A. alternata and RNA silencing proficient and deficient strains of Cryphonectria parasitica, a model filamentous fungus. AalVV1 showed symptomless infection in all of these fungal strains, even in the RNA silencing deficient C. parasitica strain.

    DOI: 10.3390/v11060577

    Web of Science

    Scopus

    PubMed

    researchmap

  • Molecular and biological characterization of a novel botybirnavirus identified from a Pakistani isolate of Alternaria alternata 査読 国際誌

    Wajeeha Shamsi, Yukiyo Sato, Atif Jamal, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki, Muhammad Faraz Bhatti

    Virus Research   263   119 - 128   2019年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier {BV}  

    DOI: 10.1016/j.virusres.2019.01.006

    Web of Science

    PubMed

    researchmap

  • "Integration of viral sequences into eukaryotic host genomes: legacy of ancient infections". 査読

    Tomonaga K, Suzuki N, Berkhout B

    Virus research   262   1   2019年3月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.virusres.2018.12.012

    Scopus

    PubMed

    researchmap

  • A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes. 査読

    Kondo H, Chiba S, Maruyama K, Andika IB, Suzuki N

    Virus research   262   37 - 47   2019年3月

  • Hijacking a host scaffold protein, RACK1, for replication of a plant RNA virus. 査読

    Hyodo K, Suzuki N, Okuno T

    The New phytologist   221 ( 2 )   935 - 945   2019年1月

  • Investigation of Host Range of and Host Defense against a Mitochondrially Replicating Mitovirus 査読

    Sabitree Shahi, Ana Eusebio-Cope, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

    Journal of Virology   93 ( 6 )   2019年1月

     詳細を見る

    出版者・発行元:American Society for Microbiology  

    DOI: 10.1128/JVI.01503-18

    Web of Science

    PubMed

    researchmap

  • The biological attributes, genome architecture and packaging of diverse multi-component fungal viruses 査読 国際誌

    Yukiyo Sato, Jos{\'{e, R Cast{\'{o } }n, Nobuhiro Suzuki

    Current Opinion in Virology   33   55 - 65   2018年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier {BV}  

    DOI: 10.1016/j.coviro.2018.07.009

    Web of Science

    PubMed

    researchmap

  • ICTV Virus Taxonomy Profile: Quadriviridae. 査読

    Chiba S, Castón JR, Ghabrial SA, Suzuki N, Ictv Report, Consortium

    The Journal of general virology   99 ( 11 )   1480 - 1481   2018年11月

  • Novel Mitoviruses and a Unique Tymo-Like Virus in Hypovirulent and Virulent Strains of the Fusarium Head Blight Fungus, Fusarium boothii 査読

    Mizutani Y, Abraham A, Uesaka K, Kondo H, Suga H, Suzuki N, Chiba S

    Viruses   10 ( 11 )   2018年10月

  • Capsid Structure of dsRNA Fungal Viruses. 査読

    Luque D, Mata CP, Suzuki N, Ghabrial SA, Castón JR

    Viruses   10 ( 9 )   2018年9月

  • ICTV virus taxonomy profile: Hypoviridae 査読

    Nobuhiro Suzuki, Said A. Ghabrial, Kook-Hyung Kim, Michael Pearson, Shin-Yi L. Marzano, Hajime Yaegashi, Jiatao Xie, Lihua Guo, Hideki Kondo, Igor Koloniuk, Bradley I. Hillman, Elliot J. Lefkowitz, Andrew J. Davison, Stuart G. Siddell, Sead Sabanadzovic, Donald B. Smith, Richard J. Orton, Peter Simmonds, ICTV Report Consortium

    Journal of General Virology   99 ( 5 )   615 - 616   2018年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology Society  

    The Hypoviridae, comprising one genus, Hypovirus, is a family of capsidless viruses with positive-sense, ssRNA genomes of 9.1–12.7 kb that possess either a single large ORF or two ORFs. The ORFs appear to be translated from genomic RNA by non-canonical mechanisms, i.e. internal ribosome entry site-mediated and stop/restart translation. Hypoviruses have been detected in ascomycetous or basidiomycetous filamentous fungi, and are considered to be replicated in host Golgi-derived, lipid vesicles that contain their dsRNA as a replicative form. Some hypoviruses induce hypovirulence to host fungi, while others do not. This is a summary of the current ICTV report on the taxonomy of the Hypoviridae, which is available at www.ictv.global/report/hypoviridae.

    DOI: 10.1099/jgv.0.001055

    Web of Science

    Scopus

    PubMed

    researchmap

  • Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus, Rosellinia necatrix: insights into evolutionary biology of fungal viruses 査読

    Juan Manuel Arjona-Lopez, Paul Telengech, Atif Jamal, Sakae Hisano, Hideki Kondo, Mery Dafny Yelin, Isabel Arjona-Girona, Satoko Kanematsu, Carlos José Lopez-Herrera, Nobuhiro Suzuki

    Environmental Microbiology   20 ( 4 )   1464 - 1483   2018年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Blackwell Publishing Ltd  

    To reveal mycovirus diversity, we conducted a search of as-yet-unexplored Mediterranean isolates of the phytopathogenic ascomycete Rosellinia necatrix for virus infections. Of seventy-nine, eleven fungal isolates tested RNA virus-positive, with many showing coinfections, indicating a virus incidence of 14%, which is slightly lower than that (approximately 20%) previously reported for extensive surveys of over 1000 Japanese R. necatrix isolates. All viral sequences were fully or partially characterized by Sanger and next-generation sequencing. These sequences appear to represent isolates of various new species spanning at least 6 established or previously proposed families such as Partiti-, Hypo-, Megabirna-, Yado-kari-, Fusagra- and Fusarividae, as well as a newly proposed family, Megatotiviridae. This observation greatly expands the diversity of R. necatrix viruses, because no hypo-, fusagra- or megatotiviruses were previously reported from R. necatrix. The sequence analyses showed a rare horizontal gene transfer event of the 2A-like protease domain between a dsRNA (phlegivirus) and a positive-sense, single-stranded RNA virus (hypovirus). Moreover, many of the newly detected viruses showed the closest relation to viruses reported from fungi other than R. necatrix, such as Fusarium spp., which are sympatric to R. necatrix. These combined results imply horizontal virus transfer between these soil-inhabitant fungi.

    DOI: 10.1111/1462-2920.14065

    Web of Science

    Scopus

    PubMed

    researchmap

  • First evidence for internal ribosomal entry sites in diverse fungal virus genomes 査読

    Sotaro Chiba, Atif Jamal, Nobuhiro Suzuki

    mBio   9 ( 2 )   2018年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    In contrast to well-established internal ribosomal entry site (IRES)-mediated translational initiation in animals and plants, no IRESs were established in fungal viral or cellular RNAs. To identify IRES elements in mycoviruses, we developed a luciferase-based dual-reporter detection system in Cryphonectria parasitica, a model filamentous fungus for virus-host interactions. A bicistronic construct entails a codon-optimized Renilla and firefly luciferase (ORluc and OFluc, respectively) gene, between which potential IRES sequences can be inserted. In this system, ORluc serves as an internal control, while OFluc represents IRES activity. Virus sequences in the 5= untranslated regions (UTRs) of the genomes of diverse positive-sense singlestranded RNA and double-stranded RNA (dsRNA) viruses were analyzed. The results show relatively high IRES activities for Cryphonectria hypovirus 1 (CHV1) and CHV2 and faint but measurable activity for CHV3. The weak IRES signal of CHV3 may be explained by its monocistronic nature, differing from the bicistronic nature of CHV1 and CHV2. This would allow these three hypoviruses to have similar rates of translation of replication-associated protein per viral mRNA molecule. The importance of 24 5=-proximal codons of CHV1 as well as the 5= UTR for IRES function was confirmed. Furthermore, victoriviruses and chrysoviruses tested IRES positive, whereas mycoreoviruses, partitiviruses, and quadriviruses showed similar Fluc activities as the negative controls. Overall, this study represents the first development of an IRES identification system in filamentous fungi based on the codon-optimized dual-luciferase assay and provides evidence for IRESs in filamentous fungi. IMPORTANCE Cap-independent, internal ribosomal entry site (IRES)-mediated translational initiation is often used by virus mRNAs and infrequently by cellular mRNAs in animals and plants. However, no IRESs have been established in fungal virus RNAs or cellular RNAs in filamentous fungi. Here, we report the development of a dualluciferase assay system and measurement of the IRES activities of fungal RNA viruses in a model filamentous fungal host, Cryphonectria parasitica. Viruses identified as IRES positive include hypoviruses (positive-sense RNA viruses, members of the expanded Picornavirus supergroup), totiviruses (nonsegmented dsRNA viruses), and chrysoviruses (tetrasegmented dsRNA viruses). No IRES activities were observed in the 5= untranslated regions of mycoreoviruses (11-segmented dsRNA viruses), quadriviruses (tetrasegmented dsRNA viruses), or partitiviruses (bisegmented dsRNA viruses). This study provides the first evidence for IRES activities in diverse RNA viruses in filamentous fungi and is a first step toward identifying trans-acting host factors and cis-regulatory viral RNA elements.

    DOI: 10.1128/mBio.02350-17

    Web of Science

    Scopus

    PubMed

    researchmap

  • A fungal Argonaute interferes with RNA interference. 査読 国際誌

    Nguyen Q, Iritani A, Ohkita S, Vu BV, Yokoya K, Matsubara A, Ikeda KI, Suzuki N, Nakayashiki H

    Nucleic acids research   46 ( 5 )   2495 - 2508   2018年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/nar/gkx1301

    PubMed

    researchmap

  • A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in an unrelated dsRNA virus: Taxonomic and evolutionary considerations 査読

    Sakae Hisano, Rui Zhang, Md. Iqbal Faruk, Hideki Kondo, Nobuhiro Suzuki

    Virus Research   244   75 - 83   2018年1月

     詳細を見る

    記述言語:英語   出版者・発行元:Elsevier B.V.  

    Recent studies illustrate that fungi as virus hosts provides a unique platform for hunting viruses and exploring virus/virus and virus/host interactions. Such studies have revealed a number of as-yet-unreported viruses and virus/virus interactions. Among them is a unique intimate relationship between a (+)ssRNA virus, yado-kari virus (YkV1) and an unrelated dsRNA virus, yado-nushi virus (YnV1). YkV1 dsRNA, a replicated form of YkV1, and RNA-dependent RNA polymerase, are trans-encapsidated by the capsid protein of YnV1. While YnV1 can complete its replication cycle, YkV1 relies on YnV1 for its viability. We previously proposed a model in which YkV1 diverts YnV1 capsids as the replication sites. YkV1 is neither satellite virus nor satellite RNA, because YkV1 appears to encode functional RdRp and enhances YnV1 accumulation. This represents a unique mutualistic virus/virus interplay and similar relations in other virus/host fungus systems are detectable. We propose to establish the family Yadokariviridae that accommodates YkV1 and recently discovered viruses phylogenetically related to YkV1. This article overviews what is known and unknown about the YkV1/YnV1 interactions. Also discussed are the YnV1 Phytoreo_S7 and YkV1 2A-like domains that may have been captured via horizontal transfer during the course of evolution and are conserved across extant diverse RNA viruses. Lastly, evolutionary scenarios are envisioned for YkV1 and YnV1.

    DOI: 10.1016/j.virusres.2017.11.006

    Web of Science

    Scopus

    PubMed

    researchmap

  • ICTV virus taxonomy profile: Partitiviridae 査読

    Eeva J. Vainio, Sotaro Chiba, Said A. Ghabrial, Edgar Maiss, Marilyn Roossinck, Sead Sabanadzovic, Nobuhiro Suzuki, Jiatao Xie, Max Nibert, Elliot J. Lefkowitz, Andrew J. Davison, Stuart G. Siddell, Donald B. Smith, Richard J. Orton, Peter Simmonds, ICTV Report Consortium

    Journal of General Virology   99 ( 1 )   17 - 18   2018年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology Society  

    The Partitiviridae is a family of small, isometric, non-enveloped viruses with bisegmented double-stranded (ds) RNA genomes of 3-4.8 kbp. The two genome segments are individually encapsidated. The family has five genera, with characteristic hosts for members of each genus: either plants or fungi for genera Alphapartitivirus and Betapartitivirus, fungi for genus Gammapartitivirus, plants for genus Deltapartitivirus and protozoa for genus Cryspovirus. Partitiviruses are transmitted intracellularly via seeds (plants), oocysts (protozoa) or hyphal anastomosis, cell division and sporogenesis (fungi)
    there are no known natural vectors. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Partitiviridae, which is available at www.ictv.global/report/partitiviridae.

    DOI: 10.1099/jgv.0.000985

    Web of Science

    Scopus

    PubMed

    researchmap

  • Viruses of Plant-Interacting Fungi 査読

    Bradley I. Hillman, Aulia Annisa, Nobuhiro Suzuki

    Advances in Virus Research   100   99 - 116   2018年1月

     詳細を見る

    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Academic Press Inc.  

    Plant-associated fungi are infected by viruses at the incidence rates from a few % to over 90%. Multiple viruses often coinfect fungal hosts, and occasionally alter their phenotypes, but most of the infections are asymptomatic. Phenotypic alterations are grouped into two types: harmful or beneficial to the host fungi. Harmful interactions between viruses and hosts include hypovirulence and/or debilitation that are documented in a number of phytopathogenic fungi, exemplified by the chestnut blight, white root rot, and rapeseed rot fungi. Beneficial interactions are observed in a limited number of plant endophytic and pathogenic fungi where heat tolerance and virulence are enhanced, respectively. Coinfections of fungi provided a platform for discoveries of interesting virus/virus interactions that include synergistic, as in the case for those in plants, and unique antagonistic and mutualistic interactions between unrelated RNA viruses. Also discussed here are coinfection-induced genome rearrangements and frequently observed coinfections by the simplest positive-strand RNA virus, the mitoviruses.

    DOI: 10.1016/bs.aivir.2017.10.003

    Web of Science

    Scopus

    PubMed

    researchmap

  • Acquisition of functions on the outer capsid surface during evolution of double-stranded RNA fungal viruses 査読

    Carlos P. Mata, Daniel Luque, Josue Gomez-Blanco, Javier M. Rodriguez, Jose M. Gonzalez, Nobuhiro Suzuki, Said A. Ghabrial, Jose L. Carrascosa, Benes L. Truss, Jose R. Caston

    PLOS PATHOGENS   13 ( 12 )   e1006755   2017年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Unlike their counterparts in bacterial and higher eukaryotic hosts, most fungal viruses are transmitted intracellularly and lack an extracellular phase. Here we determined the cryo-EM structure at 3.7 angstrom resolution of Rosellinia necatrix quadrivirus 1 (RnQV1), a fungal double-stranded (ds) RNA virus. RnQV1, the type species of the family Quadriviridae, has a multipartite genome consisting of four monocistronic segments. Whereas most dsRNA virus capsids are based on dimers of a single protein, the similar to 450-angstrom-diameter, T = 1 RnQV1 capsid is built of P2 and P4 protein heterodimers, each with more than 1000 residues. Despite a lack of sequence similarity between the two proteins, they have a similar a-helical domain, the structural signature shared with the lineage of the dsRNA bluetongue virus-like viruses. Domain insertions in P2 and P4 preferential sites provide additional functions at the capsid outer surface, probably related to enzyme activity. The P2 insertion has a fold similar to that of gelsolin and profilin, two actin-binding proteins with a function in cytoskeleton metabolism, whereas the P4 insertion suggests protease activity involved in cleavage of the P2 383-residue C-terminal region, absent in the mature viral particle. Our results indicate that the intimate virus-fungus partnership has altered the capsid genome-protective and/or receptor-binding functions. Fungal virus evolution has tended to allocate enzyme activities to the virus capsid outer surface.

    DOI: 10.1371/journal.ppat.1006755

    Web of Science

    PubMed

    researchmap

  • A possible occurrence of genome reassortment among bipartite rhabdoviruses 査読

    Hideki Kondo, Keisuke Hirota, Kazuyuki Maruyama, Ida Bagus An'dika, Nobuhiro Suzuki

    VIROLOGY   508   18 - 25   2017年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Orchid fleck virus (OFV) represents a rhabdovirus with a unique bipartite genome. OFV genetic diversity at the whole genome level has not been described. Using the partial genome sequence of RNA1, we have determined that several OFV isolates derived from orchids in Japan belong to two genetically distant subgroups: subgroup I, the members of which are distributed worldwide but previously not known in Asia, and subgroup II, which is commonly distributed in Japan. However, complete genome sequence analysis of a novel Japanese subgroup I isolate revealed that although its RNA1 sequence differs considerably from those of subgroup II isolates, its RNA2 sequence is almost identical to them. Based on phylogenetic and recombination analyses, the genome reassortment events were predicted to occur between OFV subgroups including other unseen strains. Our data show that genome reassortment contributes to the genetic diversities of the bipartite rhabdoviruses and its occurrence may be geographically constrained.

    DOI: 10.1016/j.virol.2017.04.027

    Web of Science

    researchmap

  • Roles of superoxide anion and hydrogen peroxide during replication of two unrelated plant RNA viruses in Nicotiana benthamiana 査読

    Kiwamu Hyodo, Nobuhiro Suzuki, Kazuyuki Mise, Tetsuro Okuno

    Plant Signaling and Behavior   12 ( 6 )   e1338223   2017年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Taylor and Francis Inc.  

    Reactive oxygen species (ROS), including superoxide anion (O2 −), hydrogen peroxide (H2O2), and hydroxyl radical, act as signaling molecules to transduce biotic and abiotic stimuli into stress adaptations in plants. A respiratory burst oxidase homolog B of Nicotiana benthamiana (NbRBOHB) is responsible for O2 − production to inhibit pathogen infection during plant innate immunity. RBOH-derived O2 − can be immediately converted into H2O2 by the action of superoxide dismutase. Interestingly, we recently showed that red clover necrotic mosaic virus (RCNMV), a plant positive-strand RNA [(+)RNA] virus, hijacks the host's ROS-generating machinery during infection. An RCNMV replication protein associates with NbRBOHB and triggers intracellular ROS bursts. These bursts are required for robust viral RNA replication. However, what types of ROS are required for viral replication is currently unknown. Here, we found that RCNMV replication was sensitive to an O2 − scavenger but insensitive to an H2O2 scavenger. Interestingly, replication of another plant (+)RNA virus, brome mosaic virus, was sensitive to both types of scavengers. These results indicate a virus-specific pattern requirement of O2 − and H2O2 for (+)RNA virus replication and suggest a conserved nature of the roles of ROS in (+)RNA virus replication.

    DOI: 10.1080/15592324.2017.1338223

    Web of Science

    Scopus

    PubMed

    researchmap

  • Heterodimers as the Structural Unit of the T=1 Capsid of the Fungal Double-Stranded RNA Rosellinia necatrix Quadrivirus 1 査読

    Daniel Luque, Carlos P. Mata, Fernando Gonzalez-Camacho, Jose M. Gonzalez, Josue Gomez-Blanco, Carlos Alfonso, German Rivas, Wendy M. Havens, Satoko Kanematsu, Nobuhiro Suzuki, Said A. Ghabrial, Benes L. Trus, Jose R. Caston

    JOURNAL OF VIROLOGY   90 ( 24 )   11220 - 11230   2016年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Most double-stranded RNA (dsRNA) viruses are transcribed and replicated in a specialized icosahedral capsid with a T = 1 lattice consisting of 60 asymmetric capsid protein (CP) dimers. These capsids help to organize the viral genome and replicative complex( es). They also act as molecular sieves that isolate the virus genome from host defense mechanisms and allow the passage of nucleotides and viral transcripts. Rosellinia necatrix quadrivirus 1 (RnQV1), the type species of the family Quadriviridae, is a dsRNA fungal virus with a multipartite genome consisting of four monocistronic segments (segments 1 to 4). dsRNA-2 and dsRNA-4 encode two CPs (P2 and P4, respectively), which coassemble into similar to 450-angstrom-diameter capsids. We used three-dimensional cryo-electron microscopy combined with complementary biophysical techniques to determine the structures of RnQV1 virion strains W1075 and W1118. RnQV1 has a quadripartite genome, and the capsid is based on a single-shelled T = 1 lattice built of P2-P4 dimers. Whereas the RnQV1-W1118 capsid is built of full-length CP, P2 and P4 of RnQV1-W1075 are cleaved into several polypeptides, maintaining the capsid structural organization. RnQV1 heterodimers have a quaternary organization similar to that of homodimers of reoviruses and other dsRNA mycoviruses. The RnQV1 capsid is the first T = 1 capsid with a heterodimer as an asymmetric unit reported to date and follows the architectural principle for dsRNA viruses that a 120-subunit capsid is a conserved assembly that supports dsRNA replication and organization.
    IMPORTANCE
    Given their importance to health, members of the family Reoviridae are the basis of most structural and functional studies and provide much of our knowledge of dsRNA viruses. Analysis of bacterial, protozoal, and fungal dsRNA viruses has improved our understanding of their structure, function, and evolution, as well. Here, we studied a dsRNA virus that infects the fungus Rosellinia necatrix, an ascomycete that is pathogenic to a wide range of plants. Using three-dimensional cryo-electron microscopy and analytical ultracentrifugation analysis, we determined the structure and stoichiometry of Rosellinia necatrix quadrivirus 1 (RnQV1). The RnQV1 capsid is a T = 1 capsid with 60 heterodimers as the asymmetric units. The large amount of genetic information used by RnQV1 to construct a simple T = 1 capsid is probably related to the numerous virus-host and virus-virus interactions that it must face in its life cycle, which lacks an extracellular phase.

    DOI: 10.1128/JVI.01013-16

    Web of Science

    PubMed

    researchmap

  • Reprint of “Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi” 査読

    Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki

    Virus Research   219   39 - 50   2016年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier B.V.  

    The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. Powdery mildew fungi are important and widely studied obligate phytopathogenic agents, but there has been no report on mycoviruses infecting these fungi. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least ten totivirus (genus Totivirus)-like sequences, termed red clover powdery mildew-associated totiviruses (RPaTVs). The majority of these sequences shared moderate amino acid sequence identity with each other (&lt
    44%) and with other known totiviruses (&lt
    59%). Nine of these identified sequences (RPaTV1a, 1b and 2–8) resembled the genome of the prototype totivirus, Saccharomyces cerevisiae virus-L-A (ScV-L-A) in that they contained two overlapping open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA dependent RNA polymerase (RdRp), while one sequence (RPaTV9) showed similarity to another totivirus, Ustilago maydis virus H1 (UmV-H1) that encodes a single polyprotein (CP-RdRp fusion). Similar to yeast totiviruses, each ScV-L-A-like RPaTV contains a –1 ribosomal frameshift site downstream of a predicted pseudoknot structure in the overlapping region of these ORFs, suggesting that the RdRp is translated as a CP-RdRp fusion. Moreover, several ScV-L-A-like sequences were also found by searches of the transcriptome shotgun assembly (TSA) libraries from rust fungi, plants and insects. Phylogenetic analyses show that nine ScV-L-A-like RPaTVs along with ScV-L-A-like sequences derived from TSA libraries are clustered with most established members of the genus Totivirus, while one RPaTV forms a new distinct clade with UmV-H1, possibly establishing an additional genus in the family. Taken together, our results indicate the presence of diverse, novel totiviruses in the powdery mildew fungus populations infecting red clover plants in the field.

    DOI: 10.1016/j.virusres.2016.05.011

    Web of Science

    Scopus

    PubMed

    researchmap

  • The world of diverse viruses in the kingdom Fungi Foreword 査読

    Nobuhiro Suzuki

    VIRUS RESEARCH   219   1 - 1   2016年7月

     詳細を見る

    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.virusres.2016.05.022

    Web of Science

    PubMed

    researchmap

  • Characterization of a new megabirnavirus that confers hypovirulence with the aid of a co-infecting partitivirus to the host fungus, Rosellinia necatrix 査読

    Atsuko Sasaki, Hitoshi Nakamura, Nobuhiro Suzuki, Satoko Kanernatsu

    VIRUS RESEARCH   219   73 - 82   2016年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    A new virus termed Rosellinia necatrix megabirnavirus 2 (RnMBV2) was molecularly and biologically characterized. RnMBV2 was originally harbored in isolate W8 of R. necatrix co-infected with the previously reported virus Rosellinia necatrix partitivirus 1 (RnPV1). RnMBV2 has molecular features similar and different from precedent megabirnaviruses, Rosellinia necatrix megabirnavirus 1 (RnMBV1) and Sclerotinia sclerotiorum megabirnavirus 1 (SsMBV1). The two genomic segments of RnMBV2 (9.0-kbp dsRNA1 and 8.0-kbp dsRNA2) each possess two open reading frames (ORF1 and 2 on dsRNA1 and ORF3 and 4 on dsRNA2), with a well conserved 5'-long untranslated region (UTR) of 1.7-1.8 kb between the segments, and relatively short 3'-UTR. The RnMBV2 dsRNA1-coded capsid protein (CP) and RNA-dependent RNA polymerase (RdRp) show higher sequence identity to those of SsMBV1 than to those of RnMBV1, whereas the RnMBV2 ORF3-coded protein is more closely related to the counterpart of RnMBV1. No significant amino acid sequence similarity was detected among ORF4-coded sequences of the three megabirnaviruses. Virion transfection and co-culturing allowed for single and double infection of mycelial incompatible isolates W37 and W97 by RnMBV2 and/or RnPV1. Their comparative analyses showed RnMBV2 to be able to confer hypovirulence with the aid of a co-infecting RnPV1, while the individual viruses exhibited asymptomatic infections. Interestingly, RnPV1 accumulation appeared to be increased in co-infected fungal strain with two segments of RnMBV2 relative to singly infected fungal strains. Furthermore, the dispensability of RnMBV2 dsRNA2 was demonstrated to be similar to that of the other two megabirnaviruses. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.virusres.2015.12.009

    Web of Science

    PubMed

    researchmap

  • A novel betapartitivirus RnPV6 from Rosellinia necatrix tolerates host RNA silencing but is interfered by its defective RNAs 査読

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    VIRUS RESEARCH   219   62 - 72   2016年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The family Partitiviridae comprises of five genera with bi-segmented dsRNA genomes that accommodate members infecting plants, fungi or protists. All partitiviruses with only a few exceptions cause asymptomatic infections. We report the characterization of a novel betapartitivirus termed Rosellinia necatrix partitivirus 6 (RnPV6) from a field isolate of a plant pathogenic fungus, white root rot fungus. RnPV6 has typical partitivirus features: dsRNA1 and dsRNA2 are 2462 and 2499 bps in length encoding RNA dependent RNA polymerase and capsid protein. Purified particles are spherical with a diameter of 30 nm. Taking advantage of infectivity as virions, RnPV6 was introduced into a model filamentous fungal host, chestnut blight fungus to investigate virus/host interactions. Unlike other partitiviruses tested previously, RnPV6 induced profound phenotypic alterations with symptoms characterized by a reduced growth rate and enhanced pigmentation and was tolerant to host RNA silencing. In addition, a variety of defective RNAs derived from dsRNA1 appear after virion transfection. These sub-viral RNAs were shown to interfere with RnPV6 replication, at least for that of cognate segment dsRNA1. Presence of these sub-viral elements resulted in reduced symptom expression by RnPV6, suggesting their nature as defective-interfering RNAs. The features of RnPV6 are similar to but distinct from those of a previously reported alphapartitivirus, Rosellinia necatrix partitivirus 2 that is susceptible to RNA silencing. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.virusres.2015.10.017

    Web of Science

    PubMed

    researchmap

  • The victorivirus Helminthosporium victoriae virus 1905 is the primary cause of disease/hypovirulence in its natural host and a heterologous host (Reprinted from Virus Res., vol 213, pg 238-245, 2016) 査読

    Jiatao Xie, Wendy M. Havens, Yu-Hsin Lin, Nobuhiro Suzuki, Said A. Ghabrial

    VIRUS RESEARCH   219   100 - 107   2016年7月

     詳細を見る

    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    A transmissible disease of the plant pathogenic fungus Helminthosporium victoriae, the causal agent of Victoria blight of oats, was reported more than 50 years ago. Diseased, but not normal, isolates, of H. victoriae contain two distinct viruses designated according to their sedimentation values as victorivirus Helminthosporium victoriae virus 190S (HvV190S) and chrysovirus Helminthosporium victoriae 145S (HvV145S). Although a viral etiology of the disease was previously proposed, conclusive evidence was lacking. Here we present unequivocal evidence based on transfecting virus-free H. victoriae protoplasts with purified virus particles showing that HvV190S is essential for disease development. Furthermore, we show an expansion of the host range of HvV190S to include Cryphonectria parasitica and we also show similarity in a subset of phenotypic traits between HvV190S-infected RNA silencing deficient mutant (Delta dcl-2) of C. parasitica and a strain of H. victoriae. In virulence assays on detached American chestnut branches and Red Delicious apple fruits, HvV190S-infected C parasitica strain Delta dcl-2 was markedly less virulent than wild type and virus-free Delta dcl-2 C parasitica strains. Furthermore, the hypovirulent HvV190S-infected C parasitica Delta dcl-2 strain exhibited strong antifungal activity in dual culture with the plant pathogenic fungus Sclerotinia sclerotiorum. No such inhibitory activity was observed in comparable dual cultures with wild type and virus-free Delta dcl-2 C. parasitica strains. The discovery that infection with HvV190S induced a hypovirulent phenotype in a heterologous plant pathogenic host is very significant since it might be possible to convert other economically important plant pathogenic fungi to hypovirulence using HvV190S. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.virusres.2016.05.010

    Web of Science

    researchmap

  • Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi 査読

    Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki

    VIRUS RESEARCH   213   353 - 364   2016年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. Powdery mildew fungi are important and widely studied obligate phytopathogenic agents, but there has been no report on mycoviruses infecting these fungi. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least ten totivirus (genus Totivirus)-like sequences, termed red clover powdery mildew-associated totiviruses (RPaTVs). The majority of these sequences shared moderate amino acid sequence identity with each other (&lt;44%) and with other known totiviruses (&lt;59%). Nine of these identified sequences (RPaTV1a, 1b and 2-8) resembled the genome of the prototype totivirus, Saccharomyces cerevisiae virus-L-A (ScV-L-A) in that they contained two overlapping open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA dependent RNA polymerase (RdRp), while one sequence (RPaTV9) showed similarity to another totivirus, Ustilago maydis virus H1 (UmV-H1) that encodes a single polyprotein (CP-RdRp fusion). Similar to yeast totiviruses, each ScV-L-A-like RPaTV contains a-1 ribosomal frameshift site downstream of a predicted pseudoknot structure in the overlapping region of these ORFs, suggesting that the RdRp is translated as a CP-RdRp fusion. Moreover, several ScV-L-A-like sequences were also found by searches of the transcriptome shotgun assembly (TSA) libraries from rust fungi, plants and insects. Phylogenetic analyses show that nine ScV-L-A-like RPaTVs along with ScV-L-A-like sequences derived from TSA libraries are clustered with most established members of the genus Totivirus, while one RPaTV forms a new distinct Glade with UmV-H1, possibly establishing an additional genus in the family. Taken together, our results indicate the presence of diverse, novel totiviruses in the powdery mildew fungus populations infecting red clover plants in the field. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.virusres.2015.11.015

    Web of Science

    PubMed

    researchmap

  • The victorivirus Helminthosporium victoriae virus 190S is the primary cause of disease/hypovirulence in its natural host and a heterologous host 査読

    Jiatao Xie, Wendy M. Havens, Yu-Hsin Lin, Nobuhiro Suzuki, Said A. Ghabrial

    VIRUS RESEARCH   213   238 - 245   2016年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    A transmissible disease of the plant pathogenic fungus Helminthosporium victoriae, the causal agent of Victoria blight of oats, was reported more than 50 years ago. Diseased, but not normal, isolates, of H. victoriae contain two distinct viruses designated according to their sedimentation values as victorivirus Helminthosporium victoriae virus 190S (HvV190S) and chrysovirus Helminthosporium victoriae 145S (HvV145S). Although a viral etiology of the disease was previously proposed, conclusive evidence was lacking. Here we present unequivocal evidence based on transfecting virus-free H. victoriae protoplasts with purified virus particles showing that HvV190S is essential for disease development. Furthermore, we show an expansion of the host range of HvV190S to include Cryphonectria parasitica and we also show similarity in a subset of phenotypic traits between HvV190S-infected RNA silencing deficient mutant (Delta dcl-2) of C parasitica and a strain of H. victoriae. In virulence assays on detached American chestnut branches and Red Delicious apple fruits, HvV190S-infected C parasitica strain Delta dcl-2 was markedly less virulent than wild type and virus-free Delta dcl-2 C parasitica strains. Furthermore, the hypovirulent HvV190S-infected C parasitica Delta dcl-2 strain exhibited strong antifungal activity in dual culture with the plant pathogenic fungus Sclerotinia sclerotiorum. No such inhibitory activity was observed in-comparable dual cultures with wild type and virus-free Delta dcl-2 C. parasitica strains. The discovery that infection with HvV190S induced a hypovirulent phenotype in a heterologous plant pathogenic host is very significant since it might be possible to convert other economically important plant pathogenic fungi to hypovirulence using HvV190S. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.virusres.2015.12.011

    Web of Science

    PubMed

    researchmap

  • Large-scale multiplex polymerase chain reaction assay for diagnosis of viral reactivations after allogeneic hematopoietic stem cell transplantation 査読

    Natsuko Inazawa, Tsukasa Hori, Naoki Hatakeyama, Masaki Yamamoto, Yuko Yoto, Masanori Nojima, Nobuhiro Suzuki, Norio Shimizu, Hiroyuki Tsutsumi

    JOURNAL OF MEDICAL VIROLOGY   87 ( 8 )   1427 - 1435   2015年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Viral reactivations following hematopoietic stem cell transplantation are thought to result from the breakdown of both cell-mediated and humoral immunity. As a result, many viruses could be reactivated individually or simultaneously. Using a multiplex polymerase chain reaction (PCR), we prospectively examined many kinds of viral DNAs at a time in 105 patients who underwent allogeneic hematopoietic stem cell transplantation. In total, 591 whole blood samples were collected weekly from pre- to 42 days post-transplantation and the following 13 viruses were tested; herpes simplex virus 1 (HSV-1), HSV-2, varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpes virus 6 (HHV-6), HHV-7, HHV-8, adenovirus, BK virus (BKV), JC virus (JCV), parvovirus B19, and hepatitis B virus (HBV). Several viral DNAs were detected in 12 patients before hematopoietic stem cell transplantation. The detection rate gradually increased after transplantation and peaked at 21 days. The most frequently detected virus was HHV-6 (n=63; 60.0%), followed by EBV (n=11; 10.5%), CMV (n=11; 10.5%), and HHV-7 (n=9; 8.6%). Adenovirus and HBV were each detected in one patient (1.0%). Detection of HHV-6 DNA was significantly more common among patients undergoing cord blood transplantation or with steroid treatment. EBV DNA tended to be more common in patients treated with anti-thymocyte globulin. Multiplex PCR was useful for detecting many viral reactivations after hematopoietic stem cell transplantation, simultaneously. Cord blood transplantation, steroid treatment, or anti-thymocyte globulin use was confirmed to be risk factors after transplantation. J. Med. Virol. 87:1427-1435, 2015. (c) 2015 Wiley Periodicals, Inc.

    DOI: 10.1002/jmv.24161

    Web of Science

    researchmap

  • Megabirnavirus structure reveals a putative 120-subunit capsid formed by asymmetrical dimers with distinctive large protrusions 査読

    Naoyuki Miyazaki, Lakha Salaipeth, Satoko Kanematsu, Kenji Iwasaki, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   96 ( 8 )   2435 - 2441   2015年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Rosellinia necatrix megabirnavirus 1 (RnMBV1) W779 is a bi-segmented dsRNA virus and a strain of the type species Rosellinia necatrix megabirnavirus 1 of the family Megabirnaviridae. RnMBV1 causes severe reduction of both mycelial growth of Rosellinia necatrix in synthetic medium and fungal virulence to plant hosts, and thus has strong potential for virocontrol (biological control using viruses) of white rot. The structure of RnMBV1 was examined by cryo-electron microscopy and three-dimensional reconstruction at 15.7 angstrom resolution. The diameter of the RnMBV1 capsid was 520 angstrom, and the capsid was composed of 60 asymmetrical dimers in the T=1 (so-called T=2) lattice that is well conserved among dsRNA viruses. However, RnMBV1 has putatively 120 large protrusions with a width of similar to 45 angstrom and a height of similar to 50 angstrom on the virus surface, making it distinguishable from the other dsRNA viruses.

    DOI: 10.1099/vir.0.000182

    Web of Science

    PubMed

    researchmap

  • Different Dicer-like protein components required for intracellular and systemic antiviral silencing in Arabidopsis thaliana 査読

    Ida Bagus Andika, Kazuyuki Maruyama, Liying Sun, Hideki Kondo, Tetsuo Tamada, Nobuhiro Suzuki

    PLANT SIGNALING & BEHAVIOR   10 ( 8 )   e1039214   2015年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS INC  

    Eukaryotes employ RNA silencing as an innate defense system against invading viruses. Dicer proteins play the most crucial role in initiating this antiviral pathway as they recognize and process incoming viral nucleic acids into small interfering RNAs. Generally, 2 successive infection stages constitute viral infection in plants. First, the virus multiplies in initially infected cells or organs after viral transmission and then the virus subsequently spreads systemically through the vasculature to distal plant tissues or organs. Thus, antiviral silencing in plants must cope with both local and systemic invasion of viruses. In a recent study using 2 sets of different experiments, we clearly demonstrated the differential requirement for Dicer-like 4 (DCL4) and DCL2 proteins in the inhibition of intracellular and systemic infection by potato virus X in Arabidopsis thaliana. Taken together with the results of other studies, here we further discuss the functional specificity of DCL proteins in the antiviral silencing pathway.

    DOI: 10.1080/15592324.2015.1039214

    Web of Science

    PubMed

    researchmap

  • Cymbidium chlorotic mosaic virus, a new sobemovirus isolated from a spring orchid (Cymbidium goeringii) in Japan 査読

    Hideki Kondo, Shogo Takemoto, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY   160 ( 8 )   2099 - 2104   2015年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    Cymbidium chlorotic mosaic virus (CyCMV), isolated from a spring orchid (Cymbidium goeringii), was characterized molecularly. CyCMV isometric virions comprise a single, positive-strand RNA genome of 4,083 nucleotides and 30-kDa coat protein. The virus genome contains five overlapping open reading frames with a genomic organization similar to that of sobemoviruses. BLAST searches and phylogenetic analysis revealed that CyCMV is most closely related to papaya lethal yellowing virus, a proposed dicot-infecting sobemovirus (58.8 % nucleotide sequence identity), but has a relatively distant relationship to monocot-infecting sobemoviruses, with only modest sequence identities. This suggests that CyCMV is a new monocot-infecting member of the floating genus Sobemovirus.

    DOI: 10.1007/s00705-015-2460-9

    Web of Science

    PubMed

    researchmap

  • Editorial: Viruses threatening stable production of cereal crops 査読

    Nobuhiro Suzuki, Takahide Sasaya, Il-Ryong Choi

    FRONTIERS IN MICROBIOLOGY   6   470   2015年5月

     詳細を見る

    記述言語:英語   出版者・発行元:FRONTIERS RESEARCH FOUNDATION  

    DOI: 10.3389/fmicb.2015.00470

    Web of Science

    PubMed

    researchmap

  • 50-plus years of fungal viruses 査読

    Said A. Ghabrial, Jose R. Caston, Daohong Jiang, Max L. Nibert, Nobuhiro Suzuki

    VIROLOGY   479   356 - 368   2015年5月

     詳細を見る

    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Mycoviruses are widespread in all major taxa of fungi. They are transmitted intracellularly during cell division, sporogenesis, and/or cell-to-cell fusion (hyphal anastomosis), and thus their life cycles generally lack an extracellular phase. Their natural host ranges are limited to individuals within the same or closely related vegetative compatibility groups, although recent advances have established expanded experimental host ranges for some mycoviruses. Most known mycoviruses have dsRNA genomes packaged in isometric particles, but an increasing number of positive- or negative-strand ssRNA and ssDNA viruses have been isolated and characterized. Although many mycoviruses do not have marked effects on their hosts, those that reduce the virulence of their phytopathogenic fungal hosts are of considerable interest for development of novel biocontrol strategies. Mycoviruses that infect endophytic fungi and those that encode killer toxins are also of special interest. Structural analyses of mycoviruses have promoted better understanding of vieus assembly, function, and evolution. (C) 2015 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2015.02.034

    Web of Science

    PubMed

    researchmap

  • The chestnut blight fungus for studies on virus/host and virus/virus interactions: From a natural to a model host 査読

    Ana Eusebio-Cope, Liying Sun, Toru Tanaka, Sotaro Chiba, Shin Kasahara, Nobuhiro Suzuki

    VIROLOGY   477   164 - 175   2015年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The chestnut blight fungus, Cryphonectria parasitica, is an important plant pathogenic ascomycete. The fungus hosts a wide range of viruses and now has been established as a model filamentous fungus for studying virus/host and virus/virus interactions. This is based on the development of methods for artificial virus introduction and elimination, host genome manipulability, available host genome sequence with annotations, host mutant strains, and molecular tools. Molecular tools include subcellular distribution markers, gene expression reporters, and vectors with regulatable promoters that have been long available for unicellular organisms, cultured cells, individuals of animals and plants, and certain filamentous fungi. A comparison with other filamentous fungi such as Neurospora crassa has been made to establish clear advantages and disadvantages of C parasitica as a virus host. In addition, a few recent studies on RNA silencing vs. viruses in this fungus are introduced. (C) 2014 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2014.09.024

    Web of Science

    PubMed

    researchmap

  • Differential contributions of plant Dicer-like proteins to antiviral defences against potato virus X in leaves and roots 査読

    Ida Bagus Andika, Kazuyuki Maruyama, Liying Sun, Hideki Kondo, Tetsuo Tamada, Nobuhiro Suzuki

    PLANT JOURNAL   81 ( 5 )   781 - 793   2015年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Members of the plant Dicer-like (DCL) protein family are the critical components of the RNA-silencing pathway that mediates innate antiviral defence. The distinct antiviral role of each individual DCL protein has been established with mostly based on observations of aerial parts of plants. Thus, although the roots are closely associated with the life cycle of many plant viruses, little is known about the antiviral activities of DCL proteins in roots. We observed that antiviral silencing strongly inhibits potato virus X (PVX) replication in roots of some susceptible Solanaceae species. Silencing of the DCL4 homolog in Nicotiana benthamiana partially elevated PVX replication levels in roots. In Arabidopsis thaliana, which was originally considered a non-host plant of PVX, high levels of PVX accumulation in inoculated leaves were achieved by inactivation of DCL4, while in the upper leaves and roots, it required the additional inactivation of DCL2. In transgenic A.thaliana carrying the PVX amplicon with a green fluorescent protein (GFP) gene insertion in the chromosome (AMP243 line), absence of DCL4 enabled high levels of PVX-GFP accumulation in various aerial organs but not in the roots, suggesting that DCL4 is critical for intracellular antiviral silencing in shoots but not in roots, where it can be functionally compensated by other DCL proteins. Together, the high level of functional redundancies among DCL proteins may contribute to the potent antiviral activities against PVX replication in roots.
    Significance StatementThis study demonstrates the differential contributions of DCL proteins between leaves and roots.

    DOI: 10.1111/tpj.12770

    Web of Science

    PubMed

    researchmap

  • Detection and analysis of non-retroviral RNA virus-like elements in plant, fungal, and insect genomes. 査読 国際誌

    Hideki Kondo, Sotaro Chiba, Nobuhiro Suzuki

    Methods in molecular biology (Clifton, N.J.)   1236   73 - 88   2015年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Endogenous non-retroviral RNA like sequences (NRVSs) have been discovered in the genome of a wide range of eukaryotes. These are considered as fossil RNA viral elements integrated into host genomes by as-yet-known mechanisms, and in many cases, those fossils are estimated to be millions-of-years-old. It is likely that the number of NRVS records will increase rapidly due to the growing availability of whole-genome sequences for many kinds of eukaryotes. Discovery of the novel NRVSs and understanding of their phylogenetic relationship with modern viral relatives provide important information on deep evolutionary history of RNA virus-host interactions. In this chapter, therefore, the common strategies for the identification and characterization of endogenous NRVSs from plants, insects, and fungi are described.

    DOI: 10.1007/978-1-4939-1743-3_7

    PubMed

    researchmap

  • Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research 査読

    Max L. Nibert, Said A. Ghabrial, Edgar Maiss, Till Lesker, Eeva J. Vainio, Daohong Jiang, Nobuhiro Suzuki

    VIRUS RESEARCH   188   128 - 141   2014年8月

     詳細を見る

    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Phylogenetic analyses have prompted a taxonomic reorganization of family Partitiviridae (encapsidated, bisegmented dsRNA viruses that infect plants, fungi, or protozoa), the focus of this review. After a brief introduction to partitiviruses, the taxonomic changes are discussed, including replacement of former genera Partitivirus, Alphacryptovirus, and Betacryptovirus, with new genera Alphapartitivirus, Betapartitivirus, Gammapartitivirus, and Deltapartitivirus, as well as redistribution of species among these new genera. To round out the review, other recent progress of note in partitivirus research is summarized, including discoveries of novel partitivirus sequences by metagenomic approaches and mining of sequence databases, determinations of fungal partitivirus particle structures, demonstrations of fungal partitivirus transmission to new fungal host species, evidence for other aspects of partitivirus host interactions and host effects, and identification of other fungal or plant viruses with some similarities to partitiviruses. Some outstanding questions are also discussed. (C) 2014 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.virusres.2014.04.007

    Web of Science

    PubMed

    researchmap

  • A novel single-stranded RNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix, with similarity to hypo-like viruses 査読

    Rui Zhang, Shengxue Liu, Sotaro Chiba, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    FRONTIERS IN MICROBIOLOGY   5   360   2014年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS RESEARCH FOUNDATION  

    Here we report a biological and molecular characterization of a novel positive-sense RNA virus isolated from a field isolate (NW10) of a filamentous phytopathogenic fungus, the white root rot fungus that is designated as Rosellinia necatrix fusarivirus 1 (RnFV1). A recently developed technology using zinc ions allowed us to transfer RnFV1 to two mycelially incompatible Rosellinia necatrix strains. A biological comparison of the virus-free and -recipient isogenic fungal strains suggested that RnFV1 infects latently and thus has no potential as a virocontrol agent. The virus has an undivided positive-sense RNA genome of 6286 nucleotides excluding a poly (A) tail. The genome possesses two non-overlapping open reading frames (ORFs): a large ORF1 that encodes polypeptides with RNA replication functions and a smaller ORF2 that encodes polypeptides of unknown function. A lack of coat protein genes was suggested by the failure of virus particles from infected mycelia. No evidence was obtained by Northern analysis or classical 5'-RACE for the presence of subgenomic RNA for the downstream ORE Sequence similarities were found in amino-acid sequence between RnFV1 putative proteins and counterparts of a previously reported mycovirus, Fusarium graminearum virus 1 (FgV1). Interestingly, several related sequences were detected by BLAST searches of independent transcriptome assembly databases one of which probably represents an entire virus genome. Phylogenetic analysis based on the conserved RNA-dependent RNA polymerase showed that RnFV1, FgV1, and these similar sequences are grouped in a cluster distinct from distantly related hypoviruses. It is proposed that a new taxonomic family termed Fusariviridae be created to include RnFV1 and FgV1.

    DOI: 10.3389/fmicb.2014.00360

    Web of Science

    PubMed

    researchmap

  • Biological properties and expression strategy of rosellinia necatrix megabirnavirus 1 analysed in an experimental host, Cryphonectria parasitica 査読

    Lakha Salaipeth, Sotaro Chiba, Ana Eusebio-Cope, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   95 ( Pt 3 )   740 - 750   2014年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Rosellinia necatrix megabirnavirus 1 (RnMBV1) with a bipartite dsRNA genome (dsRNA1 and dsRNA2) confers hypovirulence to its natural host, the white root rot fungus, and is thus regarded as a potential virocontrol (biocontrol) agent. Each segment has two large ORFs: ORF1 and partially overlapping ORF2 on dsRNA1 encode the major capsid protein (CP) and RNA-dependent RNA polymerase (RdRp), whilst ORF3 and ORF4 on dsRNA2 encode polypeptides with unknown functions. Here, we report the biological and molecular characterization of this virus in the chestnut blight fungus, Cryphonectria parasitica, a filamentous fungus that has been used as a model for mycovirus research. Transfection with purified RnMBV1 particles into an RNA-silencing-defective strain (Delta dcl-2) of C. parasitica and subsequent anastomosis with the WT strain (EP155) resulted in stable persistent infection in both host strains. However, accumulation levels in the two strains were different, being similar to 20-fold higher in Delta dcl-2 than in EP155. Intriguingly, whilst RnMBV1 reduced both virulence and growth rate in Delta dcl-2, it attenuated virulence without affecting significantly other traits in EP155. Western blot analysis using antiserum against recombinant proteins encoded by either ORF1 or ORF2 demonstrated the presence of a 250 kDa protein in purified virion preparations, suggesting that RdRp is expressed as a CP fusion product via a - 1 frameshift. Antiserum against the ORF3-encoded protein allowed the detection of 150, 30 and 23 kDa polypeptides specifically in RnMBV1-infected mycelia. Some properties of an RnMBV1 mutant with genome rearrangements, which occurred after transfection of Delta dcl-2 and EP155, were also presented. This study provides an additional example of C. parasitica serving as a versatile, heterologous fungus for exploring virus host interactions and virus gene expression strategies.

    DOI: 10.1099/vir.0.058164-0

    Web of Science

    PubMed

    researchmap

  • Transcriptional mapping of the messenger and leader RNAs of orchid fleck virus, a bisegmented negative-strand RNA virus 査読

    Hideki Kondo, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    VIROLOGY   452   166 - 174   2014年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The transcriptional strategy of orchid fleck virus (OFV), which has a two-segmented negative-strand RNA genome and resembles plant nucleorhabdoviruses, remains unexplored. In this study, the transcripts of six genes encoded by OFV RNA1 and RNA2 in the poly(A)-enriched RNA fraction from infected plants were molecularly characterized. All of the OFV mRNAs were initiated at a start sequence 3'-UU-5' with one to three non-viral adenine nucleotides which were added at the 5' end of each mRNA, whereas their 3' termini ended with a 5'-AUUUAAA(U/G)AAAA(A)n-3' sequence. We also identified the presence of polyadenylated short transcripts derived from the 3'-terminal leader regions of both genomic and antigenomic strands, providing the first example of plus- and minus-strand leader RNAs in a segmented minus-strand RNA virus. The similarity in the transcriptional strategy between this bipartite OFV and monopartite rhabdoviruses, especially nucleorhabdoviruses (family Rhabdoviridae) is additional support for their close relationship. (C) 2014 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2014.01.007

    Web of Science

    PubMed

    researchmap

  • Genome rearrangement of a mycovirus Rosellinia necatrix megabirnavirus 1 affecting its ability to attenuate virulence of the host fungus 査読

    Satoko Kanematsu, Takeo Shimizu, Lakha Salaipeth, Hajime Yaegashi, Atsuko Sasaki, Tsutae Ito, Nobuhiro Suzuki

    VIROLOGY   450   308 - 315   2014年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Rosellinia necatrix megabirnavirus 1 (RnMBV1) is a bi-segmented double-stranded RNA mycovirus that reduces the virulence of the fungal plant pathogen R. necatrix. We isolated strains of RnMBV1 with genome rearrangements (RnMBV1-RS1) that retained dsRNA1, encoding capsid protein (ORF1) and RNA-dependent RNA polymerase (ORF2), and had a newly emerged segment named dsRNAS1, but with loss of dsRNA2, which contains two ORFs of unknown function. Analyses of two variants of dsRNAS1 revealed that they both originated from dsRNA1 by deletion of ORFI and partial tandem duplication of ORF2, retaining a much shorter 5' untranslated region (UTR). R. necatrix transfected with RnMBV-RS1 virions showed maintenance of virulence on host plants compared with infection with RnMBV1. This suggests that dsRNAS1 is able to be transcribed and packaged, as well as suggesting that dsRNA2, while dispensable for virus replication, is required to reduce the virulence of R. necatrix. (C) 2013 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2013.12.002

    Web of Science

    PubMed

    researchmap

  • Complete genome sequence of Habenaria mosaic virus, a new potyvirus infecting a terrestrial orchid (Habenaria radiata) in Japan 査読

    Hideki Kondo, Takanori Maeda, I. Wayan Gara, Sotaro Chiba, Kazuyuki Maruyama, Tetsuo Tamada, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY   159 ( 1 )   163 - 166   2014年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    The complete genomic sequence of Habenaria mosaic virus (HaMV), which infects terrestrial orchids (Habenaria radiata), has been determined. The genome is composed of 9,499 nucleotides excluding the 3'-terminal poly(A) tail, encoding a large polyprotein of 3,054 amino acids with the genomic features typical of a potyvirus. Putative proteolytic cleavage sites were identified by sequence comparison to those of known potyviruses. The HaMV polyprotein showed 58 % amino acid sequence identity to that encoded by the most closely related potyvirus, tobacco vein banding mosaic virus. Phylogenetic analysis of the polyprotein amino acid sequence and its coding sequences confirmed that HaMV formed a cluster with the chilli veinal mottle virus group, most of which infect solanaceous plants. These results suggest that HaMV is a distinct member of the genus Potyvirus.

    DOI: 10.1007/s00705-013-1784-6

    Web of Science

    PubMed

    researchmap

  • [Diverse double-stranded RNA viruses infecting fungi]. 査読

    Chiba S, Suzuki N

    Uirusu   64 ( 2 )   225 - 238   2014年

     詳細を見る

    記述言語:日本語   出版者・発行元:日本ウイルス学会  

    これまでに報告されている菌類ウイルス(マイコウイルス)の多くは2本鎖RNA (dsRNA)をゲノムに持つ.これは,菌類では回収が比較的容易なウイルス由来dsRNAを指標にして探索が行われてきたことと無関係ではないであろう.現在では,様々な菌類,特に植物病原菌類を対象にスクリーニングが行われ,宿主菌に対し病原性を有する或いは宿主菌の病原力を衰退させるマイコウイルスの分離・同定が盛んに行われている.この背景には,植物病原糸状菌による農作物の病害をマイコウイルスで制する,いわゆるヴァイロコントロール(ウイルスによる生物防除)を目指す研究の世界的流れがある.病原性の有無に拘らず多くのRNAウイルスが分離され,多様性に満ちた菌類のdsRNAウイルスの世界が明らかになりつつある.2014年現在, dsRNAマイコウイルスを包含するウイルス科は,国際ウイルス分類委員会で6科(Reoviridae, Totiviridae, Chrysoviridae, Partitiviridae, Megabirnaviridae, Quadriviridae)承認されている.加えて,未分類ウイルスのグループをすべて含めると,近い将来倍増する見通しである.本稿では,菌類から分離された多種多様なdsRNAウイルスを最近の知見を交えて概説する.

    DOI: 10.2222/jsv.64.225

    PubMed

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: https://jlc.jst.go.jp/DN/JLC/20008436588?from=CiNii

  • [Cryphonectria parasitica as a host of fungal viruses: a tool useful to unravel the mycovirus world]. 査読

    Suzuki N

    Uirusu   64 ( 1 )   11 - 24   2014年

  • Nyamiviridae: Proposal for a new family in the order Mononegavirales 査読

    Jens H. Kuhn, Sadia Bekal, Yingyun Cai, Anna N. Clawson, Leslie L. Domier, Marieke Herrel, Peter B. Jahrling, Hideki Kondo, Kris N. Lambert, Kathie A. Mihindukulasuriya, Norbert Nowotny, Sheli R. Radoshitzky, Urs Schneider, Peter Staeheli, Nobuhiro Suzuki, Robert B. Tesh, David Wang, Lin-Fa Wang, Ralf G. Dietzgen

    ARCHIVES OF VIROLOGY   158 ( 10 )   2209 - 2226   2013年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    Nyamanini virus (NYMV) and Midway virus (MIDWV) are unclassified tick-borne agents that infect land birds and seabirds, respectively. The recent molecular characterization of both viruses confirmed their already known close serological relationship and revealed them to be nonsegmented, single- and negative-stranded RNA viruses that are clearly related to, but quite distinct from, members of the order Mononegavirales (bornaviruses, filoviruses, paramyxoviruses, and rhabdoviruses). A third agent, soybean cyst nematode virus 1 (SbCNV-1, previously named soybean cyst nematode nyavirus), was recently found to be an additional member of this new virus group. Here, we review the current knowledge about all three viruses and propose classifying them as members of a new mononegaviral family, Nyamiviridae.

    DOI: 10.1007/s00705-013-1674-y

    Web of Science

    PubMed

    researchmap

  • Orchid fleck virus structural proteins N and P form intranuclear viroplasm like structures in the absence of viral infection 査読

    Hideki Kondo, Sotaro Chiba, Ida Bagus Andika, Kazuyuki Maruyama, Tetsuo Tamada, Nobuhiro Suzuki

    Journal of Virology   87 ( 13 )   7423 - 7434   2013年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Orchid fleck virus (OFV) has a unique two-segmented negative-sense RNA genome that resembles that of plant nucleorhabdoviruses. In infected plant cells, OFV and nucleorhabdoviruses induce an intranuclear electron-lucent viroplasm that is believed to be the site for virus replication. In this study, we investigated the molecular mechanism by which OFV viroplasms are produced in vivo. Among OFV-encoded proteins, the nucleocapsid protein (N) and the putative phosphoprotein (P) were present in nuclear fractions of OFV-infected Nicotiana benthamiana plants. Transient coexpression of N and P, in the absence of virus infection, was shown to be sufficient for formation of an intranuclear viroplasm-like structure in plant cells. When expressed independently as a fluorescent protein fusion product in uninfected plant cells, N protein accumulated throughout the cell, while P protein accumulated in the nucleus. However, the N protein, when coexpressed with P, was recruited to a subnuclear region to induce a large viroplasm-like focus. Deletion and substitution mutagenesis demonstrated that the P protein contains a nuclear localization signal (NLS). Artificial nuclear targeting of the N-protein mutant was insufficient for formation of viroplasm-like structures in the absence of P. A bimolecular fluorescence complementation assay confirmed interactions between the N and P proteins within subnuclear viroplasm-like foci and interactions of two of the N. benthamiana importin-α homologues with the P protein but not with the N protein. Taken together, our results suggest that viroplasm formation by OFV requires nuclear accumulation of both the N and P proteins, which is mediated by P-NLS, unlike nucleorhabdovirus viroplasm utilizing the NLS on protein N. © 2013, American Society for Microbiology.

    DOI: 10.1128/JVI.00270-13

    Web of Science

    Scopus

    PubMed

    researchmap

  • A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia necatrix, Is Infectious as Particles and Targeted by RNA Silencing 査読

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   87 ( 12 )   6727 - 6738   2013年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    A novel victorivirus, termed Rosellinia necatrix victorivirus 1 (RnVV1), was isolated from a plant pathogenic ascomycete, white root rot fungus Rosellinia necatrix, coinfected with a partitivirus. The virus was molecularly and biologically characterized using the natural and experimental hosts (chestnut blight fungus, Cryphonectria parasitica). RnVV1 was shown to have typical molecular victorivirus attributes, including a monopartite double-stranded RNA genome with two open reading frames (ORFs) encoding capsid protein (CP) and RNA-dependent RNA polymerase (RdRp), a UAAUG pentamer presumed to facilitate the coupled termination/reinitiation for translation of the two ORFs, a spherical particle structure similar to 40 nm in diameter, and moderate levels of CP and RdRp sequence identity (34 to 58%) to those of members of the genus Victorivirus within the family Totiviridae. A reproducible transfection system with purified RnVV1 virions was developed for the two distinct fungal hosts. Transfection assay with purified RnVV1 virions combined with virus elimination by hyphal tipping showed that the effects of RnVV1 on the phenotype of the natural host were negligible. Interestingly, comparison of the RNA silencing-competent (standard strain EP155) and -defective (Delta dcl-2) strains of C. parasitica infected with RnVV1 showed that RNA silencing acted against the virus to repress its replication, which was restored by coinfection with hypovirus or transgenic expression of an RNA silencing suppressor, hypovirus p29. Phenotypic changes were observed in the Delta dcl-2 strain but not in EP155. This is the first reported study on the host range expansion of a Totiviridae member that is targeted by RNA silencing.

    DOI: 10.1128/JVI.00557-13

    Web of Science

    researchmap

  • A second quadrivirus strain from the phytopathogenic filamentous fungus Rosellinia necatrix 査読

    Yu-Hsin Lin, Sakae Hisano, Hajime Yaegashi, Satoko Kanematsu, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY   158 ( 5 )   1093 - 1098   2013年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    We report the biological and molecular characterisation of a second quadrivirus strain termed Rosellinia necatrix quadrivirus 1 strain W1118 (RnQV1-W1118) from the ascomycete white root rot fungus. Commonalities with the first quadrivirus (RnQV1-W1075) include its quadripartite genome structure, spherical particle morphology, sequence heterogeneity in the extreme terminal end, 72-82% sequence identity between the corresponding proteins, and its ability to cause a latent infection. Distinguishing features include different conserved terminal sequences and the degree of susceptibility of two major capsid proteins to proteolytic degradation, which is thought to occur during virion purification. Identification of this second quadrivirus strain strengthens our earlier proposal that "Rosellinia necatrix quadrivirus 1" should be considered the type species of a novel family, "Quadriviridae".

    DOI: 10.1007/s00705-012-1580-8

    Web of Science

    PubMed

    researchmap

  • Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system 査読

    Zoe Sanchez, Akio Tani, Nobuhiro Suzuki, Reiko Kariyama, Hiromi Kumon, Kazuhide Kimbara

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   115 ( 3 )   326 - 331   2013年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    A new multichannel microdevice flow system with stainless steel flow chamber was used for architecture visualization, development monitoring and structural quantification of GFP-labeled Pseudomonas aeruginosa PAO1 live biofilms. Direct in situ investigations using confocal laser scanning microscopy (CLSM) at 72 h revealed structural pattern differences as a result of nutrient concentration gradients. When grown in LB medium, round, dispersed cellular aggregates were formed whereas in 1/3-diluted LB medium, biofilms were mostly flat and compact. However, COMSTAT analyses showed no considerable differences in biomass and thickness between the two LB concentrations. Characterization of time-dependent development of biofilms grown in 1/3-diluted LB medium showed full maturation of colonies by 120 h reaching maximum biomass at 17.1 mu m(3)/mu m(2) and average thickness at 44.4 mu m. Consequent thinning and formation of openings through interior in colonies occurred by 168 h. These results suggest that the new system tested allowed a fast and thick biofilm development on the surface of the stainless steel flow chamber. These findings may provide better estimates of biofilm activity and systematic evaluation of the effects of different parameters on biofilm morphology and development in industrial and biomedical systems. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2012.09.018

    Web of Science

    researchmap

  • Effects of Defective Interfering RNA on Symptom Induction by, and Replication of, a Novel Partitivirus from a Phytopathogenic Fungus, Rosellinia necatrix 査読

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   87 ( 4 )   2330 - 2341   2013年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    A novel mycovirus termed Rosellinia necatrix partitivirus 2 (RnPV2), isolated from a phytopathogenic fungus, Rosellinina necatrix strain W57, was molecularly and biologically characterized in both natural and experimental host fungi. Three double-stranded RNA (dsRNA) segments, dsRNA1, dsRNA2, and defective interfering dsRNA1 (DI-dsRNA1), whose sizes were approximately 2.0, 1.8, and 1.7 kbp, respectively, were detected in W57. While the dsRNA2 sequence, encoding the coat protein, was reported previously, dsRNA1 and DI-dsRNA1 were shown to encode competent and defective (truncated) RNA-dependent RNA polymerase, respectively. Artificial introduction of RnPV2 into an RNA silencing-defective, Dicer-like 2 knockout mutant (Delta dcl-2) of a nonnatural host, Cryphonectria parasitica (chestnut blight fungus), resulted in successful infection by the DI-dsRNA1-carrying and -free RnPV2. The DI-dsRNA1-free RnPV2 strain was characterized by a higher ratio of accumulation of the intact dsRNA1 to dsRNA2, enhanced replication and severer symptom expression, compared with the DI-carrying strain. These findings confirmed the nature of DI-dsRNA1 as a DI-RNA. Both viral strains replicated to higher levels in a Delta dcl-2 mutant than in a wild-type C. parasitica fungal strain (EP155) and induced severe symptoms in the Delta dcl-2 mutant but subtle symptoms in EP155, indicating that the host RNA silencing targets the partitivirus. No obvious phenotypic effects of infection by either virus strain were detected in the natural host fungus. These combined results represent the first example of a partitivirus with DI-RNA that alters viral symptom induction in a host-dependent manner.

    DOI: 10.1128/JVI.02835-12

    Web of Science

    PubMed

    researchmap

  • Evidence for negative-strand RNA virus infection in fungi 査読

    Hideki Kondo, Sotaro Chiba, Kazuhiro Toyoda, Nobuhiro Suzuki

    Virology   435 ( 2 )   201 - 209   2013年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Fungal viruses comprise two groups: a major group of five families with double-stranded RNA genomes and a minor group with positive-sense single-stranded (ss)RNA genomes. Although many fungal viruses have been identified, no negative-stranded (-)ssRNA mycoviruses have been reported. Here we present two lines of evidence suggesting the presence of (-)ssRNA viruses in filamentous fungi based on an exhaustive search using extant (-)ssRNA viruses as queries. This revealed (-)ssRNA virus L protein-like sequences in the genome of a phytopathogenic obligate ascomycete, Erysiphe pisi. A similar search for (-)ssRNA viruses in fungal transcriptome shotgun assembly libraries demonstrated that two independent libraries from Sclerotinia homoeocarpa, another phytopathogenic ascomycete, contained several sequences considered to correspond to the entire mononegavirus L gene and likely originating from an infecting (-)ssRNA virus. These results provide strong evidence for both ancient and extant (-)ssRNA virus infections in fungi. © 2012 Elsevier Inc.

    DOI: 10.1016/j.virol.2012.10.002

    Web of Science

    Scopus

    PubMed

    researchmap

  • Viruses of the white root rot fungus, Rosellinia necatrix. 査読 国際誌

    Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    Advances in virus research   86   177 - 214   2013年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Rosellinia necatrix is a filamentous ascomycete that is pathogenic to a wide range of perennial plants worldwide. An extensive search for double-stranded RNA of a large collection of field isolates led to the detection of a variety of viruses. Since the first identification of a reovirus in this fungus in 2002, several novel viruses have been molecularly characterized that include members of at least five virus families. While some cause phenotypic alterations, many others show latent infections. Viruses attenuating the virulence of a host fungus to its plant hosts attract much attention as agents for virocontrol (biological control using viruses) of the fungus, one of which is currently being tested in experimental fields. Like the Cryphonectria parasitica/viruses, the R. necatrix/viruses have emerged as an amenable system for studying virus/host and virus/virus interactions. Several techniques have recently been developed that enhance the investigation of virus etiology, replication, and symptom induction in this mycovirus/fungal host system.

    DOI: 10.1016/B978-0-12-394315-6.00007-6

    Web of Science

    PubMed

    researchmap

  • A novel quadripartite dsRNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix 査読

    Yu-Hsin Lin, Sotaro Chiba, Akio Tani, Hideki Kondo, Atsuko Sasaki, Satoko Kanematsu, Nobuhiro Suzuki

    VIROLOGY   426 ( 1 )   42 - 50   2012年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Here we report the biological and molecular attributes of a novel dsRNA virus isolated from Rosellinia necatrix, a filamentous phytopathogenic fungus. The virus, termed Rosellinia necatrix quadrivirus 1 (RnQV1), forms rigid spherical particles approximately 45 nm in diameter in infected mycelia. The particles contain 4 dsRNA segments, dsRNA1 to dsRNA4, with a size range of 4.9 to 3.7 kbp, each possessing a single large ORF. A comparison of the virus-infected and -cured isogenic fungal strains suggested that RnQV1 infection has no appreciable phenotypic effects. Phylogenetic analysis using the dsRNA3-encoded RdRp sequence revealed that RnQV1 is more distantly related to quadripartite chrysoviruses than to monopartite totiviruses, and is placed in a distinct group from other mycoviruses. No significant sequence similarities were evident between known proteins and RnQV1 structural proteins shown to be encoded by dsRNA2 or dsRNA4. These suggest that RnQV1 is a novel latent virus, belonging to a new family. (C) 2012 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2012.01.013

    Web of Science

    PubMed

    researchmap

  • Mycoreovirus genome alterations: similarities to and differences from rearrangements reported for other reoviruses 査読

    Toru Tanaka, Ana Eusebio-Cope, Liying Sun, Nobuhiro Suzuki

    FRONTIERS IN MICROBIOLOGY   3   186   2012年

     詳細を見る

    記述言語:英語   出版者・発行元:FRONTIERS RESEARCH FOUNDATION  

    The family Reoviridae is one of the largest virus families with genomes composed of 9-12 double-stranded RNA segments. It includes members infecting organisms from protists to humans. It is well known that reovirus genomes are prone to various types of genome alterations including intragenic rearrangement and reassortment under laboratory and natural conditions. Recently distinct genetic alterations were reported for members of the genus Mycoreovirus, Mycoreovirus 1 (MyRV1), and MyRV3 with 11 (S1-S11) and 12 genome segments (S1-S12), respectively. While MyRV3 S8 is lost during subculturing of infected host fungal strains, MyRV1 rearrangements undergo alterations spontaneously and inducibly. The inducible MyRV1 rearrangements are different from any other previous examples of reovirus rearrangements in their dependence on an unrelated virus factor, a multifunctional protein, p29, encoded by a distinct virus Cryphonectria parasitica hypovirus 1 (CHV1). A total of 5 MyRV1 variants with genome rearranged segments (S1-S3, S6 and S10) are generated in the background of a single viral strain in the presence of CHV1 p29 supplied either transgenically or by coinfection. MyRV1 S4 and S10 are rearranged, albeit very infrequently, in a CHV1 p29 independent fashion. A variant of MyRV1 with substantial deletions in both S4 and S10, generated through a combined reassortment and rearrangement approach, shows comparable replication levels to the wild-type MyRV1. In vivo and in vitro interactions of CHV1 p29 and MyRV1VP9 are implicated in the induction of MyRV1 rearrangements. However, the mechanism underlying p29-mediated rearrangements remains largely unknown. MyRV1 S4 rearrangements spontaneously occurred independently of CHV1 p29. In the absence of reverse genetics systems for mycoreoviruses, molecular and biological characterization of these MyRV1 and MyRV3 variants contribute to functional analyses of the protein products encoded by those rearranged segments.

    DOI: 10.3389/fmicb.2012.00186

    Web of Science

    PubMed

    researchmap

  • Rearrangements of mycoreovirus 1 S1, S2 and S3 induced by the multifunctional protein p29 encoded by the prototypic hypovirus Cryphonectria hypovirus 1 strain EP713 査読

    Toru Tanaka, Liying Sun, Kouhei Tsutani, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   92 ( Pt 8 )   1949 - 1959   2011年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Mycoreovirus 1 (MyRV1), a member of the family Reoviridae possessing a genome consisting of 11 dsRNA segments (S1-S11), infects the chestnut blight fungus and reduces its virulence (hypovirulence). Studies have previously demonstrated reproducible induction of intragenic rearrangements of MyRV1 S6 (S6L: almost full-length duplication) and S10 (S10ss: internal deletion of three-quarters of the ORF), mediated by the multifunctional protein p29 encoded by the prototype hypovirus, Cryphonectria hypovirus 1 (CHV1) strain EP713, of the family Hypoviridae with ssRNA genomes. The current study showed that CHV1 p29 also induced rearrangements of the three largest MyRV1 segments, S1, S2 and S3, which encode structural proteins. These rearranged segments involved in-frame extensions of almost two-thirds of the ORFs (S1L, S2L and S3L, respectively), which is rare for a reovirus rearrangement. MyRV1 variants carrying S1L, S2L or S3L always contained S10ss (MyRV1/S1L+S10ss2, MyRV1/S2L+S10ss2 or MyRV1/S3L+S10ss2). Levels of mRNAs for the rearranged and co-existing unaltered genome segments in fungal colonies infected with each of the MyRV1 variants appeared to be comparable to those for the corresponding normal segments in wild-type MyRV1-infected colonies, suggesting that the rearranged segments were fully competent for packaging and transcription. Protein products of the rearranged segments were detectable in fungal colonies infected with S2L MyRV1/S2L+S10ss2 and S3L MyRV1/S3L+S10ss2, whilst S1L-encoded protein remained undetectable. S1L, S2L and S3L were associated with enhancement of the aerial hyphae growth rate. This study has provided additional examples of MyRV1 intragenic rearrangements induced by p29, and suggests that normal S1, S2 and S3 are required for the symptoms caused by MyRV1.

    DOI: 10.1099/vir.0.031138-0

    Web of Science

    PubMed

    researchmap

  • Widespread endogenization of genome sequences of non-retroviral rna viruses into plant genomes

    Sotaro Chiba, Hideki Kondo, Akio Tani, Daisuke Saisho, Wataru Sakamoto, Satoko Kanematsu, Nobuhiro Suzuki

    PLoS Pathogens   7 ( 7 )   2011年7月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    Non-retroviral RNA virus sequences (NRVSs) have been found in the chromosomes of vertebrates and fungi, but not plants. Here we report similarly endogenized NRVSs derived from plus-, negative-, and double-stranded RNA viruses in plant chromosomes. These sequences were found by searching public genomic sequence databases, and, importantly, most NRVSs were subsequently detected by direct molecular analyses of plant DNAs. The most widespread NRVSs were related to the coat protein (CP) genes of the family Partitiviridae which have bisegmented dsRNA genomes, and included plant- and fungus-infecting members. The CP of a novel fungal virus (Rosellinia necatrix partitivirus 2, RnPV2) had the greatest sequence similarity to Arabidopsis thaliana ILR2, which is thought to regulate the activities of the phytohormone auxin, indole-3-acetic acid (IAA). Furthermore, partitivirus CP-like sequences much more closely related to plant partitiviruses than to RnPV2 were identified in a wide range of plant species. In addition, the nucleocapsid protein genes of cytorhabdoviruses and varicosaviruses were found in species of over 9 plant families, including Brassicaceae and Solanaceae. A replicase-like sequence of a betaflexivirus was identified in the cucumber genome. The pattern of occurrence of NRVSs and the phylogenetic analyses of NRVSs and related viruses indicate that multiple independent integrations into many plant lineages may have occurred. For example, one of the NRVSs was retained in Ar. thaliana but not in Ar. lyrata or other related Camelina species, whereas another NRVS displayed the reverse pattern. Our study has shown that single- and double-stranded RNA viral sequences are widespread in plant genomes, and shows the potential of genome integrated NRVSs to contribute to resolve unclear phylogenetic relationships of plant species. © 2011 Chiba et al.

    DOI: 10.1371/journal.ppat.1002146

    Scopus

    PubMed

    researchmap

  • Rice Dwarf Viruses with Dysfunctional Genomes Generated in Plants Are Filtered Out in Vector Insects: Implications for the Origin of the Virus 査読

    Yingying Pu, Akira Kikuchi, Yusuke Moriyasu, Masatoshi Tomaru, Yan Jin, Haruhisa Suga, Kyoji Hagiwara, Fusamichi Akita, Takumi Shimizu, Osamu Netsu, Nobuhiro Suzuki, Tamaki Uehara-Ichiki, Takahide Sasaya, Taiyun Wei, Yi Li, Toshihiro Omura

    JOURNAL OF VIROLOGY   85 ( 6 )   2975 - 2979   2011年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Rice dwarf virus (RDV), with 12 double-stranded RNA (dsRNA) genome segments (S1 to S12), replicates in and is transmitted by vector insects. The RDV-plant host-vector insect system allows us to examine the evolution, adaptation, and population genetics of a plant virus. We compared the effects of long-term maintenance of RDV on population structures in its two hosts. The maintenance of RDV in rice plants for several years resulted in gradual accumulation of nonsense mutations in S2 and S10, absence of expression of the encoded proteins, and complete loss of transmissibility. RDV maintained in cultured insect cells for 6 years retained an intact protein-encoding genome. Thus, the structural P2 protein encoded by S2 and the nonstructural Pns10 protein encoded by S10 of RDV are subject to different selective pressures in the two hosts, and mutations accumulating in the host plant are detrimental in vector insects. However, one round of propagation in insect cells or individuals purged the populations of RDV that had accumulated deleterious mutations in host plants, with exclusive survival of fully competent RDV. Our results suggest that during the course of evolution, an ancestral form of RDV, of insect virus origin, might have acquired the ability to replicate in a host plant, given its reproducible mutations in the host plant that abolish vector transmissibility and viability in nature.

    DOI: 10.1128/JVI.02147-10

    Web of Science

    PubMed

    researchmap

  • [Mycoviruses and virocontrol]. 査読

    Chiba S, Kondo H, Kanematsu S, Suzuki N

    Uirusu   60 ( 2 )   163 - 176   2010年12月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2222/jsv.60.163

    PubMed

    researchmap

  • Complexity in Influenza Virus Targeted Drug Design: Interaction with Human Sialidases 査読

    Leonard M. G. Chavas, Ryuichi Kato, Nobuhiro Suzuki, Mark von Itzstein, Maretta C. Mann, Robin J. Thomson, Jeffrey C. Dyason, Jennifer McKimm-Breschkin, Paola Fusi, Cristina Tringali, Bruno Venerando, Guido Tettamanti, Eugenio Monti, Soichi Wakatsuki

    JOURNAL OF MEDICINAL CHEMISTRY   53 ( 7 )   2998 - 3002   2010年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    With the global spread of the pandemic H1N1 and the ongoing pandemic potential of the H5N1 subtype, the influenza virus represents one of the most alarming viruses spreading worldwide. The influenza virus sialidase is an effective drug target, and a number of inhibitors are clinically effective against the virus (zanamivir, oseltamivir, peramivir). Here we report structural and biochemical studies of the human cytosolic sialidase Neu2 with influenza virus sialidase-targeting drugs and related compounds.

    DOI: 10.1021/jm100078r

    Web of Science

    PubMed

    researchmap

  • Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction 査読

    Ana Eusebio-Cope, Liying Sun, Bradley I. Hillman, Nobuhiro Suzuki

    VIROLOGY   397 ( 2 )   399 - 408   2010年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Rearrangements of two segments, S6 and S10, of Mycoreovirus 1 (MyRV1), a member of the family Reoviridae, were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssRNA virus, the prototype hypovirus CHV1-EP713 (Sun and Suzuki, RNA 14, 2557-2571, 2008). Here we report the occurrence of rearrangements of MyRV1 S4. albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80-90% deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 Plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. This is the first example of a reovirus variant that carries deletions of over 75% of the ORFs in two genome segments and is still replication-competent. (C) 2009 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2009.11.035

    Web of Science

    PubMed

    researchmap

  • Overexpression of microRNA395c or 395e affects differently the seed germination of arabidopsis thaliana under stress conditions 査読

    Joo Yeol Kim, Hwa Jung Lee, Hyun Ju Jung, Kazuyuki Maruyama, Nobuhiro Suzuki, Hunseung Kang

    Planta   232 ( 6 )   1447 - 1454   2010年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The Arabidopsis genome encodes six members of microRNA395 (miR395) family previously determined to regulate the expression of ATP sulfurylase (APS) and the sulfate transporter SULTR2
    1. However, the mRNA targets for the individual miR395 family members and the biological consequences produced by target gene regulation of each miR395 remain to be identified. In this study, a transgenic approach was employed to determine the mRNA targets for each miR395 family member as well as the role each member plays in plant growth under abiotic stress conditions. Over-expression of miR395c or miR395e retarded and accelerated, respectively, the seed germination of Arabidopsis under high salt or dehydration stress conditions. Despite a single nucleotide difference between miR395c and miR395e, the cleavage of mRNA targets,APS1,APS3, APS4 and SULTR2
    1, was not same in miR395c- and miR395e-overexpressing plants. These results demonstrate that a given miRNA family containing a single nucleotide difference can guide the cleavage of various mRNA targets, thereby acting as a positive or negative regulator of seed germination under stress. © Springer-Verlag 2010.

    DOI: 10.1007/s00425-010-1267-x

    Scopus

    PubMed

    researchmap

  • A Novel Bipartite Double-Stranded RNA Mycovirus from the White Root Rot Fungus Rosellinia necatrix: Molecular and Biological Characterization, Taxonomic Considerations, and Potential for Biological Control 査読

    Sotaro Chiba, Lakha Salaipeth, Yu-Hsin Lin, Atsuko Sasaki, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   83 ( 24 )   12801 - 12812   2009年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    White root rot, caused by the ascomycete Rosellinia necatrix, is a devastating disease worldwide, particularly in fruit trees in Japan. Here we report on the biological and molecular properties of a novel bipartite double-stranded RNA (dsRNA) virus encompassing dsRNA-1 (8,931 bp) and dsRNA-2 (7,180 bp), which was isolated from a field strain of R. necatrix, W779. Besides the strictly conserved 5&apos; (24 nt) and 3&apos; (8 nt) terminal sequences, both segments show high levels of sequence similarity in the long 5&apos; untranslated region of approximately 1.6 kbp. dsRNA-1 and -2 each possess two open reading frames (ORFs) named ORF1 to -4. Although the protein encoded by 3&apos;-proximal ORF2 on dsRNA-1 shows sequence identities of 22 to 32% with RNA-dependent RNA polymerases from members of the families Totiviridae and Chrysoviridae, the remaining three virus-encoded proteins lack sequence similarities with any reported mycovirus proteins. Phylogenetic analysis showed that the W779 virus belongs to a separate clade distinct from those of other known mycoviruses. Purified virions similar to 50 nm in diameter consisted of dsRNA-1 and -2 and a single major capsid protein of 135 kDa, which was shown by peptide mass fingerprinting to be encoded by dsRNA-1 ORF1. We developed a transfection protocol using purified virions to show that the virus was responsible for reduction of virulence and mycelial growth in several host strains. These combined results indicate that the W779 virus is a novel bipartite dsRNA virus with potential for biological control (virocontrol), named Rosellinia necatrix megabirna-virus 1 (RnMBV1), that possibly belongs to a new virus family.

    DOI: 10.1128/JVI.01830-09

    Web of Science

    PubMed

    researchmap

  • Characteristics of Hemophagocytic Lymphohistiocytosis in Neonates: A Nationwide Survey in Japan 査読

    Nobuhiro Suzuki, Akira Morimoto, Shouichi Ohga, Kazuko Kudo, Yasushi Ishida, Eiichi Ishii

    JOURNAL OF PEDIATRICS   155 ( 2 )   235 - 238   2009年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MOSBY-ELSEVIER  

    Objective To assess the etiology, prognosis, and appropriate treatment of hemophagocytic lymphohistiocytosis (HLH) in neonates.
    Study design We collected information on neonates in whom HLH was diagnosed between 1997 and 2007 from participating members of the Japanese Society of Pediatric Hematology.
    Results HLH was diagnosed in 20 patients within 4 weeks after birth. Of the diagnostic criteria for HLH-2004, the incidence of fever was quite low in preterm infants, and hypertriglyceridemia and neutropenia were uncommon. Familial HLH (n = 6) or severe combined immunodeficiency-associated HLH (n = 1) was diagnosed in 7 patients, and 2 of them have survived. Herpes simplex virus-associated HLH was diagnosed in 6 patients, and 2 of them have survived. The overall survival rate for the 20 patients was 40%.
    Conclusions HLH is rare in neonates and has a poor prognosis. Early diagnosis and immediate treatment are required when considering the possibility of herpes simplex virus-associated or familial HLH. (J Pediatr 2009; 155:235-8).

    DOI: 10.1016/j.jpeds.2009.02.050

    Web of Science

    PubMed

    researchmap

  • Coupled termination/reinitiation for translation of the downstream open reading frame B of the prototypic hypovirus CHV1-EP713 査読

    Li-hua Guo, Liying Sun, Sotaro Chiba, Hiroyuki Araki, Nobuhiro Suzuki

    NUCLEIC ACIDS RESEARCH   37 ( 11 )   3645 - 3659   2009年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Cryphonectria hypovirus 1 (CHV1), associated with the picorna-like superfamily, infects the chestnut blight fungus and attenuates the virulence of the host fungus. The genomic RNA of the virus has two continuous open reading frames, A and B, separated by the pentanucleotide UAAUG. We present here evidence suggesting that ORF B is translated from genome-sized virus mRNA by a coupled termination/reinitiation mechanism mediated by the pentamer. In the coupled translation, the overlapping UAA and AUG triplets serve as the stop codon of ORF A and the initiator of ORF B, respectively. This was established by the use of a luciferase assay with a basic construct containing the ORF A sequence and the firefly luciferase gene while retaining the pentamer between the two coding sequences. The proportion of ribosomes reinitiating translation after terminating was determined to be 2.54.4 by three independent assay systems in fungal and insect cells. Use of a series of mutant constructs identified two sequence elements, the pentamer and the p40 sequence, that affect the efficiency of coupled translation and virus replication. Together, these results provide the first example of coupled translation facilitated by the pentanucleotide UAAUG in the kingdom Fungi. The mechanism by which the preceding p40-coding sequence promotes reinitiation is discussed.

    DOI: 10.1093/nar/gkp224

    Web of Science

    PubMed

    researchmap

  • Occurrence of the African subgroup (Ia) of BK polyomavirus in younger Japanese children 査読

    Kaori Tanaka, Tsukasa Hori, Naoki Hatakeyama, Masaki Yamamoto, Rumiko Takayama, Yuko Yoto, Nobuhiro Suzuki, Toshiaki Hayashi, Yukiho Ikeda, Hiroshi Ikeda, Tadao Ishida, Hiroyuki Tsutsumi

    MICROBIOLOGY AND IMMUNOLOGY   53 ( 6 )   319 - 322   2009年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    BK polyomavirus (BKV) is ubiquitous among humans, usually infecting them asymptomatically during childhood. BKV persists in renal tissue of individuals and their progeny are excreted in urine, particularly in immunocompromised patients. JC virus, another human polyomavirus, has been considered to be transmitted from parents to children during prolonged cohabitation. However, BKV has been supposed to be transmitted not only within but also outside the family. In the present study, to clarify this possibility, we analyzed phylogenetically 35 BKV which were excreted in the urine by Japanese children and adults undergoing stem cell transplantation. Subtypes I, III and IV were detected in 15, two and one children and in 15, one and one adults, respectively. Among 15 subtype I isolates from children, three, four and eight belonged to subgroups Ia, Ib-1 and Ic, respectively. All the three children from whom Ia was detected were less than 9 years old. In contrast in the adults, three subtype I belonged to Ib-1 and the other 12 to Ic. These findings may reflect the recent transmission of BKV Ia strains to Japanese children.

    DOI: 10.1111/j.1348-0421.2009.00129.x

    Web of Science

    PubMed

    researchmap

  • Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica 査読

    Ana Eusebio-Cope, Nobuhiro Suzuki, Hamid Sadeghi-Garmaroodi, Masatoki Taga

    FUNGAL GENETICS AND BIOLOGY   46 ( 4 )   342 - 351   2009年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The karyotypes of nine strains including three transformants of the chestnut blight fungus Cryphonectria parasitica were analyzed by pulsed-field gel electrophoresis (PFGE) and cytology using a fluorescence microscope. Cytology of the mitotic metaphase showed n = 9 for both standard strain EP155 and field strain GH2 infected by Cryphonectria hypovirus 3. Chromosomes were morphologically characterized by size, heterochromatic segment, and constriction. PFGE resolved 5 or 6 chromosomal DNA bands ranging from 3.3 Mbp to 9.7 Mbp, but accurate determination of the chromosome number was hampered by clumping of some bands. Banding profiles in PFGE were similar among the strains except for GH2, in which a chromosome translocation was detected by Southern blot analysis. By integrating the data from cytology and PFGE, the genome size of C parasitica was estimated to be ca. 50 Mbp. This is the first report of a cytological karyotype in the order Diaporthales. (C) 2009 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.fgb.2009.01.005

    Web of Science

    PubMed

    researchmap

  • Intragenic rearrangements of a mycoreovirus induced by the multifunctional protein p29 encoded by the prototypic hypovirus CHV1-EP713 査読

    Liying Sun, Nobuhiro Suzuki

    RNA-A PUBLICATION OF THE RNA SOCIETY   14 ( 12 )   2557 - 2571   2008年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT  

    Mycoreovirus 1 (MyRV1), a member of the Reoviridae family possessing a genome consisting of 11 dsRNA segments (S1-S11), and the prototype hypovirus (CHV1-EP713) of the Hypoviridae family, which is closely related to the monopartite picorna-like superfamily with a ssRNA genome, infect the chestnut blight fungus and cause virulence attenuation and distinct phenotypic alterations in the host. Here, we present evidence for reproducible induction of intragenic rearrangements of MyRV1 S6 and S10, mediated by the multifunctional protein p29 encoded by CHV1. S6 and S10 underwent an almost full-length ORF duplication (S6L) and an internal deletion of three-fourths of the ORF (S10ss). No significant influence on symptom induction in the fungal host was associated with the S6L rearrangement. In contrast, S10-encoded VP10, while nonessential for MyRV1 replication, was shown to contribute to virulence reduction and reduced growth of aerial mycelia. Furthermore, p29 was found to copurify with MyRV1 genomic RNA and bind to VP9 in vitro and in vivo, suggesting direct interactions of p29 with the MyRV1 replication machinery. This study provides the first example of a viral factor involved in RNA genome rearrangements of a different virus and shows its usefulness as a probe into the mechanism of replication and symptom expression of a heterologous virus.

    DOI: 10.1261/rna.1125408

    Web of Science

    researchmap

  • Characterization of mutants of the chestnut blight fungus (Cryphonectria parasitica) with unusual hypovirus symptoms 査読

    Md. Iqbal Faruk, Masatoshi Izumimoto, Nobuhiro Suzuki

    JOURNAL OF GENERAL PLANT PATHOLOGY   74 ( 6 )   425 - 433   2008年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER TOKYO  

    The type virus of the family Hypoviridae, Cryphonectria hypovirus 1 strain EP713 (CHV1-EP713), infects Cryphonectria parasitica, the filamentous causal fungus of chestnut blight, and reduces its virulence. This pathosystem serves as a model to study fungus-mycovirus interactions. We previously developed a genetic screening protocol for host factors associated with symptom induction by CHV1-EP713 and its mutants. In the procedure the standard field fungal isolate EP155 was transformed by cDNA from a mild hypovirus mutant Cys(72), launching virus infection, and mutagenized by random plasmid insertion with pHygR conferring hygromycin resistance. We now report an extension of the study to characterize different mutant strains, with different phenotypes than their parental strain TCys(72)-1. TCys(72)-1 is moderately reduced in pigmentation and sporulation compared to the uninfected wild-type strain EP155. Mutants sfb1, sfb2 and k202 were characterized biologically and molecularly in comparison to the previously isolated mutant (namA) and the parental strain. These mutants harbored one (sfb1) or more copies (sfb2 and k202) of the mutagenic plasmid, pHygR. The three mutants had similar biological attributes; that is, vegetative growth rate, conidiation and virulence (assay on apples) was reduced on potato dextrose agar media, relative to the parental strain and pigmentation was the same or slightly increased. Interestingly, viral dsRNA accumulation levels were apparently unaltered in these mutants. The screening method was efficient for mining fungal mutants with unusual hypovirus symptoms. Further, characterization of the mutants provides interesting insights into symptom induction by the hypovirus.

    DOI: 10.1007/s10327-008-0121-5

    Web of Science

    researchmap

  • A host factor involved in hypovirus symptom expression in the chestnut blight fungus, Cryphonectria parasitica 査読

    M. Iqbal Faruk, Ana Eusebio-Cope, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   82 ( 2 )   740 - 754   2008年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    The prototype hypovirus CRV1-EP713 causes virulence attenuation and severe suppression of asexual sporulation and pigmentation in its host, the chestnut blight fungus, Cryphonectria parasitica. We identified a factor associated with symptom induction in C. parasitica using a transformation of C. parasitica strain EP155 with a full-length cDNA clone from a mild mutant virus strain, Cys(72). This was accomplished by using mutagenesis of the transformant fungal strain TCys(72)-1 by random integration of plasmid pHygR, conferring hygromycin resistance. The mutant, nam4 (after nami-gata, meaning wave shaped), showed an irregular fungal morphology with reduced conidiation and pigmentation while retaining similar levels of virulence and virus accumulation relative to TCys(72)-1- or Cys (72)-infected strain EP155. However, the colony morphology of virus-cured namA (VC-namA) was indistinguishable from those of EP155 and virus-cured TCys(72)-1 [VC-TCys(72)-1]. The phenotypic difference between VC-namA and VC-TCys(72)-1 was found only when these strains infected with the wild type or certain mutant CHV1-EP713 strains but not when infected with Mycoreoviras 1. Sequence analysis of inverse-PCR-amplified genomic DNA fragments and cDNA identified the insertion site of the mutagenic plasmid in exon 8 of the nam-1 gene. NAM-1, comprising 1,257 amino acids, shows sequence similarities to counterparts from other filamentous fungi and possesses the CorA domain that is conserved in a class of Mg2+ transporters from prokaryotes and eukaryotes. Complementation assays using the wild-type and mutant alleles and targeted disruption of nam-1 showed that nam-1 with an extension of the pHygR-derived sequence contributed to the altered phenotype in the namA mutant. The molecular mechanism underlying virus-specific fungal symptom modulation in VC-namA is discussed.

    DOI: 10.1128/JVI.02015-07

    Web of Science

    researchmap

  • Baculovirus expression of the 11 mycoreovirus-1 genome segments and identification of the guanylyltransferase-encoding segment

    S. Supyani, Bradley I. Hillman, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   88   342 - 350   2007年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    The type member Mycoreovirus 1 (MyRV-1) of a newly described genus, Mycoreovirus, isolated from a hypovirulent strain 9B21 of the chestnut blight fungus, has a genome composed of 11 dsRNA segments (S1-S11). All of the segments have single ORFs on their capped, positive-sense strands. Infection of insect cells by baculovirus recombinants carrying full-length cDNAs of S1-S1 1 resulted in overexpression of protein products of the expected sizes, based on their deduced amino acid sequences. This expression system was utilized to identify the S3-encoded protein (VP3) as a guanylyltransferase by an autoguanylylation assay, in which only VP3 was radiolabelled with [alpha-(32) P]GTP. A series of progressive N-terminal and C-terminal deletion mutants was made to localize the autoguanylylation-active site of VP3 to aa residues 133-667. Within this region, a sequence stretch (aa 170-250) with relatively high sequence similarity to homologues of two other mycoreoviruses and two coltivinuses was identified. Site-directed mutagenesis of conserved aa residues revealed that H233, H242, Y243, F244 and F246, but not K172 or K202, play critical roles in guanylyltransferase activities. Together with broader sequence alignments of 'turreted' reoviruses, these results supported the a/vxxHx(8)Hyf/lvf motif, originally noted for orthoreovirus and aquareoviruses, as an active site for guanylyltransferases of viruses within the Orthoreovirus, Aquareovirus, Cypovirus, Oryzavirus, Fijivirus, Coltivirus and Mycoreovirus genera, as well as for the proposed Dinovernavirus genus.

    DOI: 10.1099/vir.0.82318-0

    Web of Science

    researchmap

  • Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713 査読

    Liying Sun, Donald L. Nuss, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   87   3703 - 3714   2006年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Infection of the chestnut blight fungus, Cryphonectria parasitica, by the prototypic hypovirus Cryphonectria hypovirus 1-EP713 (CHV1-EP713) or by the type member, Mycoreovirus 1-Cp9B21 (MyRV1-Cp9B21), of a novel genus (Mycoreovirus) of the family Reoviridae results in hypovirulence, but with a different spectrum of phenotypic changes. The former virus depresses pigmentation and conidiation dramatically, whilst the latter virus has little effect on these processes. This study showed that double infection by the two viruses resulted in a phenotype similar to that of CHV1-EP713 singly infected colonies, but with further decreased levels of host conidiation and vegetative growth and increased levels of MyRV1-Cp9B21 genomic dsRNA accumulation (twofold) and vertical transmission (sixfold). In contrast, CHV1-EP713 RNA accumulation was not altered by MyRV1-Cp9B21 infection. It was also found that the papain-like cysteine protease p29, encoded by CHV1-EP713 ORF A, contributes to the phenotypic alterations and transactivation of MyRV1-Cp9B21 replication and transmission. Chromosomally expressed p29 was able to increase MyRV1-Cp9B21 vertical transmission by more than twofold and genomic RNA accumulation by 80 %. Transactivation was abolished by Cys -&gt; Gly mutations at p29 residues 70 and 72 located within the previously identified symptom-determinant domain required for suppression of host pigmentation and sporulation and p29-mediated in trans enhancement of homologous Delta p29 mutant virus RNA replication. Transactivation was not altered by Ser substitutions at the p29 protease catalytic residue Cys(162). These results indicated a link between p29-mediated enhancement of heterologous virus accumulation and transmission and p29-mediated host symptom expression. The role of p29 as a suppressor of RNA silencing is discussed.

    DOI: 10.1099/vir0.82213-0

    Web of Science

    researchmap

  • Pns4 of rice dwarf virus is a phosphoprotein, is localized around the viroplasm matrix, and forms minitubules 査読

    T. Wei, A. Kikuchi, N. Suzuki, T. Shimizu, K. Hagiwara, H. Chen, T. Omura

    Archives of Virology   151 ( 9 )   1701 - 1712   2006年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Rice dwarf virus (RDV), a member of the family Reoviridae, has a 12-segmented dsRNA genome. Seven segments, designated S1, S2, S3, S5, S7, S8, and S9, encode structural proteins, while the remainder encode nonstructural proteins. One of the nonstructural proteins, Pns4, which is encoded by S4, was characterized. Pns4 was a phosphorylatable substrate in a phosphorylation assay in vivo
    it associated with large cytoplasmic fibrils and formed novel minitubules in infected cultured cells of its leafhopper insect vector, as revealed by immunofluorescence and immunoelectron microscopy. Early in infection, Pns4 was detected at the periphery of the viroplasm, and it was then observed on amorphous or fibrillar inclusions, which were identified as bundles of minitubules, at later stages of infection. Since viroplasms are believed to be the site of RDV replication, the intracellular location of Pns4 suggests that this protein might be involved in the process of assembly of the RDV virion. © Springer-Verlag 2006.

    DOI: 10.1007/s00705-006-0757-4

    Scopus

    PubMed

    researchmap

  • The spread of Rice dwarf virus among cells of its insect vector exploits virus-induced tubular structures 査読

    Taiyun Wei, Akira Kikuchi, Yusuke Moriyasu, Nobuhiro Suzuki, Takumi Shimizu, Kyoji Hagiwara, Hongyan Chen, Mami Takahashi, Tamaki Ichiki-Uehara, Toshihiro Omura

    JOURNAL OF VIROLOGY   80 ( 17 )   8593 - 8602   2006年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Various cytopathological structures, known as inclusion bodies, are formed upon infection of cultured leafhopper cells by Rice dwarf virus, a member of the family Reoviridae. These structures include tubules of approximately 85 nm in diameter which are composed of the nonstructural viral protein Pns10 and contain viral particles. Such tubular structures were produced in heterologous non-host insect cells that expressed PnsIO of the virus. These tubules, when associated with actin-based filopodia, were able to protrude from the surface of cells and to penetrate neighboring cells. A binding assay in vitro revealed the specific binding of Pns10 to actin. Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus, even in the presence of neutralizing antibodies. However, treatment of host cells with drugs that inhibited the elongation of actin filaments abolished the extension of PnsIO tubules from the surface of cells, with a significant simultaneous decrease in the extent of infection of neighboring cells. These results together revealed a previously undescribed aspect of the intercellular spread of Rice dwarf virus, wherein the virus exploits tubules composed of a nonstructural viral protein and actin-based filopodia to move into neighboring cells.

    DOI: 10.1128/JVI.00537-06

    Web of Science

    researchmap

  • Pns12 protein of Rice dwarf virus is essential for formation of viroplasms and nucleation of viral-assembly complexes 査読

    Taiyun Wei, Takumi Shimizu, Kyoji Hagiwara, Akira Kikuchi, Yusuke Moriyasu, Nobuhiro Suzuki, Hongyan Chen, Toshihiro Omura

    Journal of General Virology   87 ( 2 )   429 - 438   2006年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cytoplasmic inclusion bodies, known as viroplasms or viral factories, are assumed to be the sites of replication of members of the family Reoviridae. Immunocytochemical and biochemical analyses were carried out to characterize the poorly understood viroplasms of the phytoreovirus Rice dwarf virus (RDV). Within 6 h of inoculation of cells, viroplasms, namely discrete cytoplasmic inclusions, were formed that contained the non-structural proteins Pns6, Pns11 and Pns12 of RDV, which appeared to be the constituents of the inclusions. Formation of similar inclusions in non-host insect cells upon expression of Pns12 in a baculovirus system and the association of molecules of Pns12 in vitro suggested that the inclusions observed in RDV-infected cells were composed basically of Pns12. Core proteins P1, P3, P5 and P7 and core virus particles were identified in the interior region of the inclusions. In contrast accumulation of the outer capsid proteins P2, P8 and P9 and of intact virus particles was evident in the peripheral regions of the inclusions. These observations suggest that core particles were constructed inside the inclusions, whereas outer capsid proteins were assembled at the periphery of the inclusions. Viral inclusions were shown to be the sites of viral RNA synthesis by labelling infected cells with 5-bromouridine 5′-tri phosphate. The number of viroplasms decreased with time post-inoculation as their sizes increased, suggesting that inclusions might fuse with one another during the virus-propagation process. Our results are consistent with a model, proposed for vertebrate reoviruses, in which viroplasms play a pivotal role in virus assembly. © 2006 SGM.

    DOI: 10.1099/vir.0.81425-0

    Scopus

    PubMed

    researchmap

  • Complete genome sequence of Mycoreovirus-1/Cp9B21, a member of a novel genus within the family Reoviridae, isolated from the chestnut blight fungus Cryphonectria parasitica 査読

    N Suzuki, S Supyani, K Maruyama, BI Hillman

    JOURNAL OF GENERAL VIROLOGY   85   3437 - 3448   2004年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Mycoreovirus 1 (MYRV-1) is the type species of the newly described genus Mycoreovirus of the large virus family Reoviridae. The virus was isolated from a hypovirulent strain (9B21) of the chestnut blight fungus, Cryphonectria parasitica. A previous study showed that double-shelled particles introduced to fungal spheroplasts resulted in stably infected colonies. Of the 11 double-stranded RNA genomic segments (S1-S11), the three largest (S1-S3) were sequenced previously and shown to have moderate levels of similarity to the homologous segments of mammal-pathogenic coltiviruses (Eyach virus and Colorado tick fever virus) and another fungus-infecting reovirus, Mycoreovirus 3 of Rosellinia necatrix strain W370 (MYRV-3/RnW370). The sequences of the remaining segments (S4-S11) are reported here. All of the segments have single ORFs on their positive strands and the terminal sequences 5'-GAUCA----GCAGUCA-3' are conserved among currently and previously sequenced segments. Oligo-cap analysis showed that the positive strands of the genomic segments are capped, whereas the negative strands are not. Similarities among the four evolutionarily related viruses include low or moderate levels of amino acid sequence identity (14(.)7-34(.)2%) and isoelectric points among equivalent polypeptides, e.g. proteins encoded by segments S4 and S5 of the four viruses. Phylogenetic analysis indicated that MYRV-1/Cp9B21 is related more closely to MYRV-3/RnW370 than to the coltiviruses. An interesting dissimilarity is found in codon-choice pattern among the four viruses, i.e. MYRV-1/Cp9B21 segments have a lower frequency of [XYG + XYC] than corresponding segments of the other viruses, suggesting a possible adjustment of virus codon usage to their host environments.

    DOI: 10.1099/vir.0.80293-0

    Web of Science

    researchmap

  • A reovirus of the fungus Cryphonectria parasitica that is infectious as particles and related to the Coltivirus genus of animal pathogens 査読

    BI Hillman, S Supyani, H Kondo, N Suzuki

    JOURNAL OF VIROLOGY   78 ( 2 )   892 - 898   2004年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    RNA viruses of filamentous fungi fall into two broad categories, those that contain double-stranded RNA (dsRNA) genomes in rigid particles and those that are more closely related to positive-sense, single-stranded RNA viruses with dsRNA replicative intermediates found within lipid vesicles. Effective infectivity systems have been described for the latter, using RNA transcripts, but not for the former. We report the characterization of a reovirus from Cryphonectria parasitica, the filamentous fungus that causes chestnut blight disease. The virus substantially reduces the virulence of the fungus and results in dramatically altered colony morphology, as well as changes in other associated fungal traits, relative to the virus-free isogenic strain. Virus particles from infected mycelium contained 11 segments of dsRNA and showed characteristics typical of the family Reoviridae. Sequences of the largest three segments revealed that the virus is closely related to the Coltivirus genus of animal pathogens, which includes the human pathogen Colorado tick fever virus. The introduction of purified virus particles into protoplasts from virus-free isolates of the fungus resulted in a newly infected mycelium with the same morphology and virus composition as the original virus-infected isolate. This represents the completion of Koch's postulates for a true dsRNA virus from a filamentous fungus and the description of a definitive fungal member of the family Reoviridae.

    DOI: 10.1128/JVI.78.2.892-898.2004

    Web of Science

    PubMed

    researchmap

  • Viruses of the Chestnut Blight Fungus, Cryphonectria parasitica

    Bradley I. Hillman, Nobuhiro Suzuki

    Advances in Virus Research   63   423 - 472   2004年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S0065-3527(04)63007-7

    Scopus

    PubMed

    researchmap

  • Hypovirus papain-like protease p29 functions in trans to enhance viral double-stranded RNA accumulation and vertical transmission

    N Suzuki, K Maruyama, M Moriyama, DL Nuss

    JOURNAL OF VIROLOGY   77 ( 21 )   11697 - 11707   2003年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    The prototypic hypovirus CHV1-EP713 attenuates virulence (hypovirulence) and alters several physiological processes of the chestnut blight fungus Cryphonectria parasitica. The papain-like protease, p29, and the highly basic protein, p40, derived, respectively, from the N-terminal and C-terminal portions of the CHV1-EP713-encoded open reading frame (ORF) A polyprotein, p69, both contribute to reduced pigmentation and sporulation. The p29 coding region was shown to suppress pigmentation and asexual sporulation in the absence of virus infection in transformed C. parasitica, whereas transformants containing the p40-coding domain exhibited a wild-type, untransformed phenotype. Deletion of either p29 or p40 from the viral genome also results in reduced accumulation of viral RNA. We now show that p29, but not p40, functions in trans to enhance genomic RNA accumulation and vertical transmission of p29 deletion mutant viruses. The frequency of virus transmission through conidia was found to decrease with reduced accumulation of viral genomic double-stranded RNA (dsRNA): from almost 100% for wild-type virus to similar to50% for Deltap29, and 10 to 20% for Deltap69. When expressed from a chromosomally integrated cDNA copy, p29 elevated viral dsRNA accumulation and transmission for Deltap29 mutant virus to the level shown by wild-type virus. Increased viral RNA accumulation levels were also observed for a Deltap69 mutant lacking almost the entire ORF A sequence. Such enhancements were not detected in transgenic fungal colonies expressing p40. Mutation of p29 residues Cys(70) or Cys(72), strictly conserved in hypovirus p29 and potyvirus HC-Pro, resulted in the loss of both p29-mediated suppressive activity in virus-free transgenic C. parasitica and in trans enhancement of RNA accumulation and transmission, suggesting a linkage between these functional activities. These results suggest that p29 is an enhancer of viral dsRNA accumulation and vertical virus transmission through asexual spores.

    DOI: 10.1128/JVI.77.21.11697-11707.2003

    Web of Science

    Scopus

    PubMed

    researchmap

  • Extending chestnut blight hypovirus host range within diaporthales by biolistic delivery of viral cDNA 査読

    A Sasaki, M Onoue, S Kanematsu, K Suzaki, M Miyanishi, N Suzuki, DL Nuss, K Yoshida

    MOLECULAR PLANT-MICROBE INTERACTIONS   15 ( 8 )   780 - 789   2002年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYTOPATHOLOGICAL SOC  

    Biolistic bombardment was used to successfully transform three phytopathogenic fungal species with an infectious cDNA clone of the prototypic hypovirus, CHV1-EP713, a genetic element responsible for the virulence attenuation (hypovirulence) of the chestnut blight fungus, Cryphonectria parasitica. The fungal species included two strains each of C. parasitica and Valsa ceratosperma, as well as one strain of Phomopsis G-type (teleomorph Diaporthe Nitschke); all are members of the order Diaporthales but classified into three different genera. A subset of transformants for each of the fungal species contained CHV1EP713 dsRNA derived from chromosomally integrated viral cDNA. As has been reported for CHV1-EP713 infection of the natural host C. parasitica, biolistic introduction of CHV1-EP713 into the new fungal hosts V. ceratosperma and Phomopsis G-type resulted in altered colony morphology and, more importantly, reduced virulence. These results suggest a potential for hypoviruses as biological control agents in plant-infecting fungal pathogens other than the chestnut blight fungus and closely related species. In addition, the particle delivery technique offers a convenient means of transmitting hypoviruses to potential host fungi that provides new avenues for fundamental mycovirus research and may have practical applications for conferring hypovirulence directly on infected plants in the field.

    DOI: 10.1094/MPMI.2002.15.8.780

    Web of Science

    PubMed

    researchmap

  • Contribution of protein p40 to hypovirus-mediated modulation of fungal host phenotype and viral RNA accumulation

    N Suzuki, DL Nuss

    JOURNAL OF VIROLOGY   76 ( 15 )   7747 - 7759   2002年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    The papain-like protease p29, derived from the N-terminal portion of the hypovirus CHV1-EP713-encoded open reading frame (ORF) A polyprotein, p69, was previously shown to contribute to reduced pigmentation and sporulation by the infected host, the chestnut blight fungus Cryphonectria parasitica, while being dispensable for virus replication and attenuation of fungal virulence (hypovirulence). We now report that deletion of the C-terminal portion of p69, which encodes the highly basic protein p40, resulted in replication-competent mutant viruses that were, however, significantly reduced in RNA accumulation. While the Deltap40 mutants retained the ability to confer hypovirulence, Deltap40-infected fungal strains produced more asexual spores than strains infected with either wild-type CHV1-EP713 or a Deltap29 mutant virus. As observed for Deltap29-infected colonies, pigment production was significantly increased in Deltap40-infected fungal strains relative to that in CHV1-EP713-infected strains. Virus-mediated suppression of laccase production was not affected by p40 deletion. A gain-of-function analysis was employed to map the p40 symptom determinant to the N-terminal domain, encompassing p69 amino acid residues Thr(288) to Arg(312). Evidence that the gain of function was due to the encoded protein rather than the corresponding RNA sequence element was provided by introducing frameshift mutations on either side of the activity determinant domain. Moreover, restoration of symptoms correlated with increased accumulation of viral RNA. These results suggest that p40 indirectly contributes to virus-mediated suppression of fungal pigmentation and conidiation by providing an accessory function in hypovirus RNA amplification. A possible role for p40 in facilitating ORF B expression and the relationship between hypovirus RNA accumulation and symptom expression are discussed.

    DOI: 10.1128/JVI.76.15.7747-7759.2002

    Web of Science

    Scopus

    PubMed

    researchmap

  • Engineering hypoviruses for fundamental and practical applications

    Donald L. Nuss, Baoshan Chen, Lynn M. Geletka, Todd B. Parsley, Nobuhiro Suzuki

    dsRNA Genetic Elements: Concepts and Applications in Agriculture, Forestry, and Medicine   145 - 163   2001年1月

     詳細を見る

    © 2002 by CRC Press LLC. The concept of using a parasite of a parasite for disease control is appealing from both an intellectual and an ecological perspective. The phenomenon of transmissible hypovirulence, in which virulence of the chestnut blight fungus, Cryphonectria parasitica, is attenuated by double-stranded RNA viruses in the family Hypoviridae, is often cited as a classic example of this approach to biological disease control. Progress in the development of an infectious cDNA-based reverse genetics system for hypoviruses has provided the means for engineering these viral agents for both fundamental and practical applications, e.g., as a tool for probing signal transduction processes underlying fungal pathogenesis and for enhanced biocontrol potential. This chapter describes the basic techniques used for manipulating hypovirus genomes and provides specific examples of how they are being applied to identify virus-encoded determinants responsible for altering fungal host phenotype, to gain insights into dispensable and essential elements of viral replication, and to fine tune the interaction between a fungal pathogen and its plant host.

    DOI: 10.1201/9781420039122

    Scopus

    researchmap

  • Essential and dispensable virus-encoded replication elements revealed by efforts to develop hypoviruses as gene expression vectors

    N Suzuki, LM Geletka, DL Nuss

    JOURNAL OF VIROLOGY   74 ( 16 )   7568 - 7577   2000年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    We have investigated whether hypoviruses, viral agents responsible for virulence attenuation (hypovirulence) of the chestnut blight fungus Cryphonectria parasitica, could serve as gene expression vectors, The infectious cDNA clone of the prototypic hypovirus CHV1-EP713 was modified to generate 20 different vector candidates. Although transient expression was achieved for a subset of vectors that contained the green fluorescent protein gene from Aequorea victoria, long-term expression (past day 8) was not observed for any vector construct. analysis of viral RNAs recovered from transfected fungal colonies revealed that the foreign genes were readily deleted from the replicating virus, although small portions of foreign sequences were retained by some vectors after months of replication, However, the results of vector viability and progeny characterization provided unexpected new insights into essential and dispensable elements of hypovirus replication. The N-terminal portion (codons 1 to 24) of the 5'-proximal open reading frame (ORF), ORF ii was found to be required for virus replication, while the remaining 598 codons of this ORF were completely dispensable. Substantial alterations were tolerated in the pentanucleotide UAAUG that contains the ORF A termination codon and the overlapping putative initiation codon of the second of the two hypovirus ORFs, ORF B, Replication competence was maintained following either a frameshift mutation that caused a two-codon extension of ORF A or a modification that produced a single-ORF genomic organization, These results are discussed in terms of determinants of hypovirus replication, the potential utility of hypoviruses as gene expression vectors, and possible mechanisms by which hypoviruses recognize and delete foreign sequences.

    DOI: 10.1128/JVI.74.16.7568-7577.2000

    Web of Science

    Scopus

    PubMed

    researchmap

  • Similarity and divergence among viruses in the genus Furovirus

    Y Shirako, N Suzuki, RC French

    VIROLOGY   270 ( 1 )   201 - 207   2000年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC  

    Nucleotide sequences of RNAs 1 and 2 of a Japanese strain of soil-borne wheat mosaic virus (sBWMV), the type species of the genus Furovirus, and sorghum chlorotic spot virus (SCSV) were determined from cloned cDNA. The relationship among the Japanese and US strains of SBWMV, SCSV, oat golden stripe virus (OGSV), and recently proposed Chinese wheat mosaic and European wheat mosaic viruses (CWMV and EWMV) were examined at the nucleotide and amino acid levels. Pairwise comparisons of genome-encoded proteins among the six viruses showed that the US strains of SBWMV and CWMV were the most closely related pair in RNA 1 and the Japanese strains of SBWMV and EWMV were most closely related in RNA 2. SCSV was most distantly related to the other five viruses. Phylogenetic analysis indicated that there may have been an ancient reassortment between RNAs 1 and 2 of the four wheat-infecting viruses and OGSV, while SCSV was shown to have separated from the rest before the other five viruses diverged. The fact that CWMV and EWMV have almost identical biological properties as well as the sequence similarities to the two strains of SBWMV suggests that they be regarded as strains of SBWMV, considering that SBWMV consists of generically diverged strains. OGSV and SCSV are distinct in biological properties in addition to genetic divergence in the genus Furovirus. (C) 2000 Academic Press.

    DOI: 10.1006/viro.2000.0251

    Web of Science

    Scopus

    PubMed

    researchmap

  • Mapping of a hypovirus p29 protease symptom determinant domain with sequence similarity to potyvirus HC-Pro protease.

    Suzuki, N, Chen, B, Nuss, D. L

    Journal of Virology   73   9478 - 9484   1999年10月

     詳細を見る

    Hypovirus infection of the chestnut blight fungus Cryphonectria parasitica results in a spectrum of phenotypic changes that can include alterations in colony morphology and significant reductions in pigmentation, asexual sporulation, and virulence (hypovirulence). Deletion of 88% [Phe(25) to Pro(243)] of the virus-encoded papain-like protease, p29, in the context of an infectious cDNA clone of the prototypic hypovirus CHV1-EP713 (recombinant virus Ap29) partially relieved virus-mediated suppression of pigmentation and sporulation without altering the level of hypovirulence. We now report mapping of the p29 symptom determinant domain to a region extending from Phe(25) through Gln(73) by a gain-of-function analysis following progressive repair of the Δp29 deletion mutant. This domain was previously shown to share sequence similarity [including conserved cysteine residues Cys(38), Cys(48), Cys(70), and Cys(72)] with the N-terminal portion of the potyvirus-encoded helper component-proteinase (HC-Pro), a multifunctional protein implicated in aphid-mediated transmission, genome amplification, polyprotein processing, long-distance movement, and suppression of posttranscriptional silencing. Substitution of a glycine residue for either Cys(38) or Cys(48) resulted in no qualitative or quantitative changes in virus-mediated symptoms. Unexpectedly, mutation of Cys(70) resulted in a very severe phenotype that included significantly reduced mycelial growth and profoundly altered colony morphology. In contrast, substitution for Cys(72) resulted in a less severe symptom phenotype approaching that observed for Δp29. The finding that p29-mediated symptom expression is influenced by two cysteine residues that are conserved in the potyvirus-encoded HC-Pro raises the possibility that these related viral-papain-like proteases function in their respective fungal and plant hosts by impacting ancestrally related regulatory pathways.

    Scopus

    PubMed

    CiNii Article

    researchmap

  • In vivo and in vitro phosphorylation of rice dwarf phytoreovirus Pns12 cytoplasmic nonstructural protein

    N Suzuki, D Hosokawa, Y Matsuura, A Kikuchi, T Omura

    ARCHIVES OF VIROLOGY   144 ( 7 )   1371 - 1380   1999年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG WIEN  

    In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12, which is encoded by one of the twelve dsRNA genome segments, S12, and comprises 312 amino acids, was investigated. When [P-32]phosphoric acid was incorporated into RDV-infected leafhopper cultured cells, labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody. Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12, a baculovirus recombinant carrying a full-length cDNA of RDV S12. Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host (rice, barley, wheat, leafhopper) cells and non-host (tobacco, spinach, white clover, S. frugiperda, mosquito, mammals) cells as well. Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells, and frequently localized in a slightly electron-dense patch. These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein.

    DOI: 10.1007/s007050050593

    Web of Science

    Scopus

    PubMed

    researchmap

  • The loss of outer capsid protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovirus

    Masatoshi Tomaru, Wakako Maruyama, Akira Kikuchi, Jin Yan, Yafeng Zhu, Nobuhiro Suzuki, Masamichi Isogai, Yuzuru Oguma, Ikuo Kimura, Toshihiro Omura

    Journal of Virology   71 ( 10 )   8019 - 8023   1997年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A transmission-defective (TD) isolate of rice dwarf phytoreovirus lacked the ability to infect cells when derived from the virus-free insect vector Nephotettix cincticeps. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified virus showed that among six structural proteins, the P2 outer capsid protein (encoded by genome segment S2) was absent from the TD isolate, whereas all six proteins were present in the transmission-competent (TC) isolate. P2 was not detected on immunoblots of rice plants infected with the TD isolate. Genome segment S2 and its transcript were detected in both TD and TC isolates. Sequence analysis of the S2 segment of the TD isolate revealed the presence of a termination codon due to a point mutation in the open reading frame, which might explain the absence of P2 in the TD isolate. These results demonstrate that the P2 protein is one of the factors essential for infection by the virus of vector cells and, thus, influences transmissibility by vector insects.

    Scopus

    PubMed

    researchmap

  • Polycistronic (tri- or bicistronic) phytoreoviral segments translatable in both plant and insect cells

    N Suzuki, M Sugawara, DL Nuss, Y Matsuura

    JOURNAL OF VIROLOGY   70 ( 11 )   8155 - 8159   1996年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Genomic segment S12 of rice dwarf virus and segment S9 of wound tumor virus, both members of the genus Phytoreovirus, have small out-of-phase overlapping open reading frames (ORFs). Western blot (immunoblot) analysis revealed that rice dwarf virus S12 mRNA specified translation products from the large ORF and two overlapping small ORFs both in rice plant hosts and in Spodoptera frugiperda insect cells. These results provide the first example of a tricistronic mRNA for a segmented double-stranded RNA virus. Similarly, wound tumor virus S9 mRNA was found to direct the synthesis of protein products from both the large ORF and small out-of-frame ORF in S. frugiperda cells. Results of site-specific and deletion mutagenesis studies were consistent with a leaky scanning translation mechanism for the synthesis of the small ORFs.

    Web of Science

    Scopus

    PubMed

    researchmap

  • Novel NTP binding property of rice dwarf phytoreovirus minor core protein P5.(共著)

    Nobuhiro Suzuki, Tomonobu Kusano, Yoshiharu Matsuura, Toshihiro Omura

    Virology   219 ( 2 )   471 - 474   1996年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Rice dwarf phytoreovirus (RDV) mRNA synthesized from purified virion has a cap structure, m7GpppAm-, which suggests the presence of guanylyltransferase activity in the virion. We attempted to identify the enzyme involved in the cap formation by using a nucleoside triphosphate binding assay. Incubation of virion with [α-32P]GTP resulted in labeling of an 89-kDa protein that had not previously been identified in purified virus preparations. Interestingly this protein also covalently bound UTP and ATP, which is not a property of the known guanylyltransferases. RDV particles catalyzed GTP-PP(i), dGTP-PP(i), ATP-PP(i), and UTP-PP(i) exchange reactions. In SDS-polyacrylamide gel electrophoresis, the 89-kDa protein comigrated with the SE-coded protein, P5, which had been expressed by a baculovirus vector. Moreover, the labeled 89-kDa protein was precipitated by an antiserum against this recombinant RDV P5. Careful reinvestigation of purified virus particles by SDS-polyacrylamide gel electrophoresis and Western blotting analyses showed that they contained a small amount of P5 (&lt;0.5% of the total protein) within the core. These results may suggest that the minor core protein of RDV, which is coded by S5, is a candidate guanylyltransferase, although the biological significance of its ATP and UTP binding activities remains largely unknown.

    DOI: 10.1006/viro.1996.0273

    Web of Science

    Scopus

    PubMed

    researchmap

  • DNA cloning, sequence analysis of segment 7 of rice dwarf virus genome and its high expression in E. coli.(共著) 査読

    Qu, L, Li, Y, Quan, S, Ding, S, Suzuki, N, Chen, Z

    Acta Microbiologica Sinica   36   335 - 343   1996年

     詳細を見る

    記述言語:中国語   掲載種別:研究論文(学術雑誌)  

    researchmap

  • A MAIZE DNA-BINDING FACTOR WITH A BZIP MOTIF IS INDUCED BY LOW-TEMPERATURE

    T KUSANO, T BERBERICH, M HARADA, N SUZUKI, K SUGAWARA

    MOLECULAR & GENERAL GENETICS   248 ( 5 )   507 - 517   1995年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER VERLAG  

    We have isolated a low temperature-induced maize gene, mlip15, via cross hydridization using rice lip19. The longest cDNA isolated comprised 1179 bp and coded for a 135 amino acid bZIP (basic region/leucine zipper) protein. The gene showed 61.4% and 68.9% identity with the rice gene at the DNA and amino acid sequence levels, respectively, and is distinct from other maize genes that code for bZIP proteins. The level of mlip15 transcript was positively regulated by low temperature in the same way as the lip19 transcript. The levels of the transcript were also strongly increased by salt stress and exogenous abscisic acid, and slightly increased by anaerobiosis, but were not affected by heat shock and drought. The mLIP15 protein and truncated derivatives, produced in rabbit reticulocyte lysates or in an Escherichia coli expression system, were able to bind to a fragment of the wheat histone H3 gene promoter. This binding was diminished by addition of a molar excess of the hexamer sequence 5'-ACGTCA-3' found in the promoter and of the G-box-like sequence, but not by the addition of the ocs sequence or a mutated hexamer sequence. The factor bound to a promoter region of the maize Adh1 gene, expression of which is also induced by low temperature. These results lead to the conclusion that mlip15 is a strong candidate for a low temperature-induced transcription factor in maize.

    DOI: 10.1007/BF02423445

    Web of Science

    Scopus

    PubMed

    researchmap

  • イネ萎縮ウイルスゲノムの構造解析

    鈴木 信弘

    日本植物病理學會報   61 ( 3 )   176 - 176   1995年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    DOI: 10.3186/jjphytopath.61.176

    CiNii Article

    CiNii Books

    researchmap

  • Molecular analysis of the rice dwarf virus genome

    Nobuhiro Suzuki

    Seminars in Virology   6 ( 2 )   89 - 95   1995年

     詳細を見る

    記述言語:英語  

    DOI: 10.1006/smvy.1995.0011

    Scopus

    researchmap

  • NUCLEOTIDE-SEQUENCE OF RICE DWARF PHYTOREOVIRUS GENOME SEGMENT-2 - COMPLETION OF SEQUENCE ANALYSES OF RICE DWARF VIRUS

    UYEDA, I, N SUDA, N YAMADA, H KUDO, K MURAO, H SUGA, KIMURA, I, E SHIKATA, Y KITAGAWA, T KUSANO, M SUGAWARA, N SUZUKI

    INTERVIROLOGY   37 ( 1 )   6 - 11   1994年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    The complete nucleotide sequence of rice dwarf phytoreovirus genome segment 2 (S2) was determined to be 3,512 nucleotides long with one open reading frame initiating at nucleotide 15 and terminating at nucleotide 3363. The encoded polypeptide was predicted to have 1,116 residues with a relative molecular weight of 123 kD. Comparison of S2 of two isolates showed they had identical lengths and 97 and 98.3% nucleotide and amino acid sequence identities, respectively. A search of the Swiss-Prot data base (R 22.0) failed to fmd any proteins with significant homology to the S2-encoded protein. Determination of the nucleotide sequence of the S2 has completed the sequence determination of the genome of rice dwarf virus. Homology searches made for proteins encoded by each of the genomic segments showed that the polypeptide encoded by S11 has similarity to histone H1 protein and VP6 of blue tongue virus, indicating it might possess nucleic acid binding properties.

    DOI: 10.1159/000150348

    Web of Science

    Scopus

    PubMed

    researchmap

  • Immunodetection of Rice Dwarf Phytoreoviral Proteins in Both Insect and Plant Hosts

    Nobuhiro Suzuki, Mikiko Sugawara, Tomonobu Kusano, Hajime Mori, Yoshiharu Matsuura

    Virology   202 ( 1 )   41 - 48   1994年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Peptides encoded by truncated (S1 and S2) or full-length (S3 to S11) cDNAs of 11 of the 12 rice dwarf phytoreovirus (RDV) genome segments were expressed in a baculovirus vector system. Antibodies raised against each of the expressed peptides were used as probes to detect the authentic proteins encoded by the RDV open reading frame. Polypeptides identified as gene products of S1 to S11 in both RDV-infected rice leaf and leafhopper (Nephotettix cincticeps) homogenates were the P1 minor core (170 kDa), P2 (130 kDa), P3 major core (110 kDa), Pns4 nonstructural (83 kDa), P5 (89 kDa), Pns6 nonstructural (56 kDa), P7 minor core (58 kDa), P8 outercapsid (43 kDa), Pns9 nonstructural (49 kDa), Pns10 nonstructural (35 kDa), and Pns11a nonstructural (23 kDa) proteins. These molecular masses were in accord with those obtained from previous in vitro translation analysis. The locations of P2 and P5 remain to be determined although both of these are assumed to be outer layer proteins. Quantitative detection showed that accumulation (per gram of total proteins) of the virus-coded proteins in rice leaves is much greater (more than 15 times) than that in leafhoppers and that the content of the individual proteins varies within a sample from rice or leafhopper and also varies among different samples. © 1994 Academic Press. All rights reserved.

    DOI: 10.1006/viro.1994.1320

    Scopus

    PubMed

    researchmap

  • LOW-TEMPERATURE-DEPENDENT EXPRESSION OF A RICE GENE ENCODING A PROTEIN WITH A LEUCINE-ZIPPER MOTIF

    K AGUAN, K SUGAWARA, N SUZUKI, T KUSANO

    MOLECULAR & GENERAL GENETICS   240 ( 1 )   1 - 8   1993年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER VERLAG  

    We have isolated from rice suspension cells three non-sequence-related cDNAs the expression of which is markedly induced by low, non-freezing temperature. Here we further characterize one of the cDNA clones, lip19. Expression of lip19 is positively regulated by low temperature, but not affected by high (40-degrees-C) temperature. Sequencing and primer extension analyses showed that lip19 has a long (552 bp) 5' non-coding sequence followed by a single open reading frame specifying a protein of 148 amino acids. The deduced amino acid sequence of the protein, Lip19, shows at its amino-terminus a conserved basic region followed by a ''leucine-zipper'' domain. The reported sequence most similar to Lip19 is maize OCSBF-1, which is a bZip-type DNA binding protein. The possibility is suggested that Lip19 is a transcriptional factor that is positively controlled by low temperature.

    DOI: 10.1007/BF00276876

    Web of Science

    Scopus

    PubMed

    researchmap

  • Molecular analysis of rice dwarf phytoreovirus segment S1: Interviral homology of the putative RNA-dependent RNA polymerase between plant- and animal-infecting reoviruses

    Nobuhiro Suzuki, Masako Tanimura, Yuzuko Watanabe, Tomonobu Kusano, Yoshichika Kitagawa, Narushi Suda, Hiroshi Kudo, Ichiro Uyeda, Eishiro Shikata

    Virology   190 ( 1 )   240 - 247   1992年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We have determined the complete nucleotide sequence of the largest segment S1 of rice dwarf phytoreovirus (RDV), a member of the family Reoviridae. S1 is 4423 nucleotides long with a segment-specific inverted repeat located adjacent to the conserved termini (5′GGCAAA- - -UGAU3′). A major open reading frame (bases 36 to 4367) on the S1 plus strand, which is preceded by a minicistron (bases 6 to 29), encodes the polypeptide (P1) consisting of 1444 amino acids with a Mr of 164, 142. The sense-strand transcript derived from the full-length S1 cDNA, the minicistron of which was abolished, directed the synthesis of a polypeptide of 170 kDa in addition to smaller polypeptides in wheat germ extracts, and the 170-kDa product comigrated with the minor core protein in SDS-polyacrylamide gel. Thus, P1 is assumed to be localized in the viral core particle. The consensus sequence element conserved in RNA-dependent RNA polymerase is observed in the P1 amino acid sequence predicted from the nucleotide sequence. Based on the dendrogram established from the sequence alignment around the polymerase module region, and sequence identity within the alignment, P1 of plant-infecting RDV was evolutionarily compared with VP1, λ3, and VP1 of three other animal-infecting members of the family, rota-, reo-, and bluetongue viruses. Consequently, RDV S1 was shown to be more closely related to the rotavirus gene segment 1, in terms of molecular evolution, than the animal-infecting members are to one another. © 1992.

    DOI: 10.1016/0042-6822(92)91210-L

    Scopus

    PubMed

    researchmap

  • Rice dwarf phytoreovirus segment S12 transcript is tricistronic in Vitro

    Nobuhiro Suzuki, Mikiko Sugawara, Tomonobu Kusano

    Virology   191 ( 2 )   992 - 995   1992年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Sequence analysis revealed that rice dwarf phytoreovirus segment S12 is 1066 nucleotides long with a small out-of-phase, overlapping open reading frame (ORF) as well as a major ORF. The large ORF (positions 42 to 980) encodes 312 amino acids, while the small one (bases 313 to 591) encodes 92 amino acids with an additional in-frame AUG codon (positions 337-339) 24 nucleotides downstream from the first one. Transcripts from a full-length cDNA directed the in vitro synthesis of three polypeptides of 33 (considered to be translated from the long ORF), 8, and 7 kDa. Alteration of each of the two ATG codons on the small ORF demonstrated their involvement in the generation of the 8- and 7-kDa polypeptides. Although it is still unknown whether these proteins are expressed in vivo, the small ORF is shown to be conserved in S9s of two other members of the genus Phytoreovirus, rice gall dwarf virus and wound tumor virus, suggesting its common, important function. © 1992.

    DOI: 10.1016/0042-6822(92)90279-X

    Scopus

    PubMed

    researchmap

  • ISOLATION OF GENES FOR LOW-TEMPERATURE-INDUCED PROTEINS IN RICE BY A SIMPLE SUBTRACTIVE METHOD

    K AGUAN, K SUGAWARA, N SUZUKI, T KUSANO

    PLANT AND CELL PHYSIOLOGY   32 ( 8 )   1285 - 1289   1991年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC PLANT PHYSIOLOGISTS  

    We have isolated cDNAs that correspond to three distinct low-temperature-induced mRNAs from rice cells using an effective, simple subtraction method. The corresponding genes were designated lip (gene encoding low-temperature-induced protein) -5, -9 and -19. The expression of these genes was slightly stimulated (lip5 and lip19) or unaffected (lip9) by abscisic acid, unlike the expression of the rab16A-D genes which are readily induced by either abscisic acid or high osmotic stress. Time-course analysis revealed that while the lip5 gene was induced 3 h after temperature down-shift, and the lip9 and lip19 genes were induced 6 h after such a shift.

    Web of Science

    Scopus

    researchmap

  • MOLECULAR ANALYSIS OF RICE DWARF PHYTOREOVIRUS SEGMENT-S11 CORRESPONDING TO WOUND TUMOR PHYTOREOVIRUS SEGMENT-S12 査読

    N SUZUKI, M HARADA, T KUSANO

    JOURNAL OF GENERAL VIROLOGY   72   2233 - 2237   1991年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    The complete nucleotide sequence of rice dwarf phytoreovirus (RDV) genome segment S11 was determined. S11 is 1067 nucleotides long. There is an inverted repeat of 10 bp adjacent to the conserved 5'-terminal hexanucleotide (5' GGUAAA 3') and 3'-terminal tetranucleotide (5' UAGU 3') sequences. A single large open reading frame found in the plus strand of S11 begins with the first AUG codon (bases 6 to 8) and extends for 567 bases. Evolutionary relatedness between RDV S11 and wound tumour phytoreovirus S12 based on amino acid sequence similarity (25.8%) was found. In addition to the first AUG triplet, RDV S11 possesses a second in-phase AUG triplet (positions 30 to 32) nearby, which conforms to the Kozak consensus sequence. Two forms of the protein were identified by using an in vitro transcription and translation system in which a tailored full-length cDNA was the initial template. The abolition of the first AUG codon by site-directed mutagenesis resulted in disappearance of the larger translation product. These results strongly suggest that the two products are translated from the first and second AUG codons. Whether the two proteins are expressed in vivo is at present unclear.

    Web of Science

    researchmap

  • MOLECULAR-CLONING AND EXPRESSION OF THIOBACILLUS-FERROOXIDANS CHROMOSOMAL RIBULOSE BISPHOSPHATE CARBOXYLASE GENES IN ESCHERICHIA-COLI

    T KUSANO, K SUGAWARA, C INOUE, N SUZUKI

    CURRENT MICROBIOLOGY   22 ( 1 )   35 - 41   1991年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER VERLAG  

    The genes coding for d-ribulose-1,5-bisphosphate carboxylase (RuBPCase) from an iron-oxidizing bacterium, Thiobacillus ferrooxidans, were cloned into an Escherichia coli plasmid, pUC18. The recombinant plasmid, termed pTR11, contained a 4.0-kb PstI fragment including the entire coding regions for both large and small subunits of RuBPCase. Escherichia coli carrying pTR11 did not show any CO2-fixing activity. However, a derivative plasmid with an appropriate deletion, which was placed under the control of a tac promoter, conferred ribulose bisphosphate-dependent CO2-fixing activity on the host cell. Analysis of gel-filtration chromatography of the RuBPCase synthesized in E. coli revealed that it had a hexadecameric form like the native enzyme of T. ferrooxidans. © 1991 Springer-Verlag New York Inc.

    DOI: 10.1007/BF02106210

    Web of Science

    Scopus

    researchmap

  • Outer capsid protein heterogeneity of rice dwarf phytoreovirus

    N. Suzuki, M. Sugawara

    Journal of General Virology   72 ( 9 )   2239 - 2242   1991年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The 46K outer capsid protein encoded by RNA segment S8 and the 42K polypeptide, previously thought to be the segment S9-encoded structural protein, were isolated from a rice dwarf phytoreovirus purified preparation, and then analysed by peptide mapping and electroblot-ELlSA. Staphylococcus aureus V8 protease peptide mapping patterns of the 42K and 46K proteins were similar. Two monoclonal antibodies (MAbs), obtained after immunization with virus particles dissociated by 0.1% SDS, were each specific for both the 42K and 46K proteins. Furthermore, the MAbs bound common peptide fragments which were generated by digestion of the 42K and 46K proteins with V8 protease or proteinase K. These results strongly suggest that the 42K protein is not a gene product of S9 but a product overlapping with the 46K outer capsid protein. Whether the two proteins are functionally distinct remains to be determined.

    DOI: 10.1099/0022-1317-72-9-2239

    Scopus

    PubMed

    researchmap

  • Molecular analysis of rice dwarf phytoreovirus segment S11 corresponding to wound tumour phytoreovirus segment S12

    N. Suzuki, M. Harada, T. Kusano

    Journal of General Virology   72 ( 9 )   2233 - 2237   1991年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The complete nucleotide sequence of rice dwarf phytoreovirus (RDV) genome segment S11 was determined. S11 is 1067 nucleotides long. There is an inverted repeat of 10 bp adjacent to the conserved 5'terminal hexanucleotide (5' GGUAAA 3') and 3'terminal tetranucleotide (5' UAGU 3') sequences. A single large open reading frame found in the plus strand of S11 begins with the first AUG codon (bases 6 to 8) and extends for 567 bases. Evolutionary relatedness between RDV S11 and wound tumour phytoreovirus S12 based on amino acid sequence similarity (25-8%) was found. In addition to the first AUG triplet, RDV S11 possesses a second in-phase AUG triplet (positions 30 to 32) nearby, which conforms to the Kozak consensus sequence. Two forms of the protein were identified by using an in vitro transcription and translation system in which a tailored full-length cDNA was the initial template. The abolition of the first AUG codon by site-directed mutagenesis resulted in disappearance of the larger translation product. These results strongly suggest that the two products are translated from the first and second AUG codons. Whether the two proteins are expressed in vivo is at present unclear.

    DOI: 10.1099/0022-1317-72-9-2233

    Scopus

    PubMed

    researchmap

  • AN IMPROVED METHOD FOR THE CONSTRUCTION OF HIGH-EFFICIENCY CDNA LIBRARY IN PLASMID OR LAMBDA VECTOR 査読

    K AGUAN, T KUSANO, N SUZUKI, Y KITAGAWA

    NUCLEIC ACIDS RESEARCH   18 ( 4 )   1071 - 1071   1990年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS UNITED KINGDOM  

    DOI: 10.1093/nar/18.4.1071

    Web of Science

    Scopus

    PubMed

    researchmap

  • ISOLATION AND SEROLOGICAL COMPARISON OF VIRUS-CODED PROTEINS OF 3 POTYVIRUSES INFECTING CUCURBITACEOUS PLANTS 査読

    N SUZUKI, Y SHIRAKO, Y EHARA

    INTERVIROLOGY   31 ( 1 )   43 - 49   1990年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    Cylindrical inclusion proteins (CIPs), amorphous inclusion proteins (AIPs) and virus particles were partially purified from Cucurbita maxima leaves infected with zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus 2 (WMV2), and the watermelon mosaic virus 1 strain of papaya ringspot virus (PRSV-W). Antisera to these individual antigens, except AIPs of ZYMV and WMV2, were prepared and used to determine with electroblot-ELISA the serological differentiation indices (SDIs) between homologous and heterologous antigens. The average SDIs between ZYMV and WMV2 CIPs, between ZYMV and PRSV-W CIPs, and between WMV2 and PRSV-W CIPs were 1.5, 3.5, and 2.5, respectively. The SDIs between ZYMV and PRSV-W AIPs and between WMV2 and PRSV-W AIPs were 5 and 3, respectively. The average SDIs between ZYMV and WMV2 capsid proteins (CPs), between ZYMV and PRSV-W CPs, and between WMV2 and PRSV-W CPs were 3, 7, and 8, respectively. These data suggest that the serological relationship between ZYMV and WMV2 is much closer than that between PRSV-W and ZYMV or between PRSV-W and WMV2 and that antigenic determinants of CIP and AIP were conserved more than those of CP among the three potyviruses.

    DOI: 10.1159/000150133

    Web of Science

    Scopus

    PubMed

    researchmap

  • Sequence analysis of rice dwarf phytoreovirus genome segments S4, S5, and S6: Comparison with the equivalent wound tumor virus segments 査読

    Nobuhiro Suzuki, Yuzuko Watanabe, Tomonobu Kusano, Yoshichika Kitagawa

    Virology   179 ( 1 )   446 - 454   1990年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The complete nucleotide sequences of genome segments S4, S5, and S6 of rice dwarf phytoreovirus (RDV) were determined. S4 and S5 consist of 2468 and 2570 base pairs, respectively, S5 thus being larger in size than S4, contrary to the situation suggested by their relative migration in a polyacrylamide gel. S6 is 1699 nucleotides long. The individual segments have segment-specific inverted repeats adjacent to the conserved terminal sequences (5′GGUAAA-UGAU3′ for S4, 5′GGCAAA-UGAU3′ for S5 and S6). S4, S5, and S6 each have single long open reading frames encoding 727, 801, and 509 amino acids, respectively. A low level of amino acid sequence homology was observed between RDV S4 and wound tumor virus (WTV) S4 (22.4%), and between RDV S6 and WTV S6 (20.2%). On the other hand, RDV S5 and WTV S5 show 52.0% amino acid sequence similarity, indicating that S5 is much more conserved than any other segments of RDV and WTV reported so far. Further comparative analyses indicate that the RDV segment shows a greater frequency of usage of codons XYG and XYC, and much less frequent usage of codon XYA than the equivalent WTV segment, this codon preference bias being more conspicuous than expected from the base contents. © 1990.

    DOI: 10.1016/0042-6822(90)90313-G

    Scopus

    PubMed

    researchmap

  • Sequence analysis of the rice dwarf phytoreovirus segment s3 transcript encoding for the major structural core protein of 114 kDa 査読

    Nobuhiro Suzuki, Yuzuko Watanabe, Tomonobu Kusano, Yoshichika Kitagawa

    Virology   179 ( 1 )   455 - 459   1990年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The primary structure of rice dwarf phytoreovirus (RDV) genome segment S3 was determined. RDV S3 consists of 3195 nucleotides. A 14-bp segment-specific inverted repeat is located immediately adjacent to the conserved terminal sequence (5′GGCAAA---UGAU3′). A single long open reading frame encoding for 1019 amino acids with an Mr of 114,289 is also identified. In order to investigate the localization of the predicted polypeptide, we determined the amino acid sequence of the 26-kDa peptide fragment obtained from the structural core protein digested by Staphylococcus aureus V8 protease. The sequence of the fragment was found in the translational product presumed from the nucleotide sequence of RDV S3, indicating that RDV S3 encodes the major structural core protein of 114 kDa. © 1990.

    DOI: 10.1016/0042-6822(90)90314-H

    Scopus

    PubMed

    researchmap

  • A simple method for elimination of non-specific reactions in non-precoated and electroblot enzyme-linked immunosorbent assay procedures used for detection of zucchini yellow mosaic virus 査読

    Suzuki, N, Shirako, Y, Ehara, Y

    Annals of the Phytopathological Society of Japan   56   337 - 341   1990年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    CiNii Article

    CiNii Books

    researchmap

  • NUCLEOTIDE-SEQUENCE OF RICE DWARF VIRUS SEGMENT-5

    N SUZUKI, Y WATANABE, T KUSANO, Y KITAGAWA

    NUCLEIC ACIDS RESEARCH   17 ( 21 )   8858 - 8859   1989年11月

     詳細を見る

    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS UNITED KINGDOM  

    DOI: 10.1093/nar/17.21.8858

    Web of Science

    Scopus

    PubMed

    researchmap

  • DISTRIBUTION OF CYLINDRICAL INCLUSION, AMORPHOUS INCLUSION AND CAPSID PROTEINS OF WATERMELON MOSAIC VIRUS-2 IN SYSTEMICALLY INFECTED PUMPKIN LEAVES 査読

    N SUZUKI, T KUDO, Y SHIRAKO, Y EHARA, T TACHIBANA

    JOURNAL OF GENERAL VIROLOGY   70   1085 - 1091   1989年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Web of Science

    researchmap

  • The use of SDS-polyacrylamide gel electrophoresis to assess purification procedure of three potyviruses infecting cucurbitaceous plants. 査読

    Suzuki, N, Shirako, Y, Ehara, Y

    Annals of the Phytopathological Society of Japan   55 ( 5 )   586 - 593   1989年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:日本植物病理学会  

    ズッキーニ黄斑モザイクウイルス(ZYMV), カボチャモザイクウイルス(WMV2), パパイヤ輪点ウイルスW系統(PRSV-W)それぞれの最適精製法を確立するため, 濃縮法, 抽出用緩衝液, 清澄化剤, 懸濁用緩衝液のウイルス収量および純度に与える影響をSDS-PAGEを用いて調べた。さらに, それら3種のウイルスの理化学的性状を調べ比較した。少量の感染葉を用いて精製法を検討する場合, SDS-PAGEは極めて有効であった。3種のウイルスはTriton X-100による清澄化, 2回の分画遠心, しょ糖密度勾配(20〜50%)遠心により精製され, 収量はともに感染葉100g当り5〜10mgであった。WMV2およびPRSV-W精製試料から, 34Kの外被蛋白(CP)と分子量の異なる(27〜33K)数種のCP分解産物が若干検出された。ZYMV精製試料からは, 33KのCPと28KのCP分解産物が同程度検出された。変性条件下での1.7%アガロースゲル電気泳動の結果, WMV2とPRSV-WのRNAの分子量は等しかったが, ZYMV RNAの分子量はそれらより若干小さかった。

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: http://agriknowledge.affrc.go.jp/RN/2010450878

▼全件表示

書籍等出版物

  • Structure of dsRNA Mycoviruses. Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Caston, J. R, Suzuki, N, Ghabrial, S. A

    Academic Press, Elsevier  2021年3月 

     詳細を見る

  • An introduction to fungal viruses. Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Suzuki, N( 担当: 単著)

    2021年3月 

     詳細を見る

  • Viruses as Infectious Agents: Fungal, invertebrate, bacterial archaeal viruses Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman)

    Suzuki, N, Krell, P, Feiss, M, Prangishvili, M( 担当: 共著)

    Academic Press, Elsevier  2021年3月 

     詳細を見る

  • Megabirnaviruses (Megabirnaviridae). Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Sato, Y, Suzuki, N( 担当: 共著)

    Academic Press, Elsevier  2021年3月 

     詳細を見る

  • Quadriviruses (Quadriviridae). Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Kondo, H, Caston, J. R, Suzuki, N( 担当: 共著)

    Academic Press, Elsevier  2021年3月 

     詳細を見る

  • Yado-kari virus 1 and yado-nushi virus 1 (Unassigned). Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Das, S, Suzuki, N( 担当: 共著)

    Academic Press, Elsevier  2021年3月 

     詳細を見る

  • ネオウイルス学

    河岡, 義裕, 川口, 寧, 高橋, 英樹, 澤, 洋文, 朝長, 啓造, 松浦, 善治, 堀江, 真行, 佐藤, 佳, 鈴木, 信弘, 長崎, 慶三, 中川, 草, 野田, 岳志, 村田, 和義, 牧野, 晶子, 渡辺, 登喜子, 望月, 智弘, 大場, 靖子, 西浦, 博, 岩見, 真吾, 古瀬, 祐気( 担当: 共著 ,  範囲: 「ヤドカリ」「ヤドヌシ」と呼ばれるウイルスの共生関係)

    集英社  2021年3月  ( ISBN:9784087211597

     詳細を見る

    総ページ数:315p   記述言語:日本語

    CiNii Books

    researchmap

  • 植物病理学第2版(眞山滋志・土佐幸雄編)

    鈴木信弘・大木理・上田一郎( 担当: 分担執筆 ,  範囲: ウイルス)

    文永堂  2020年3月 

     詳細を見る

  • Integration of viral sequences into eukaryotic host genomes: legacy of ancient infections

    Tomonaga, K., Suzuki, N., and Berkhout, B.( 担当: 共編者(共編著者))

    Elsevier  2019年3月 

     詳細を見る

  • Advances in Fungal Virus Research

    Suzuki, N.( 担当: 編集)

    Elsevier  2016年 

     詳細を見る

  • Viruses threatening stable production of cereal crops.

    Suzuki, N., Sasaya, T., Choi, I-R.( 担当: 共編者(共編著者))

    Frontiers in Microbiology  2015年 

     詳細を見る

  • The Springer index of viruses Tidona, C. Buchen-Osmond, G. Darai (eds.)

    Suzuki, N., and Kanematsu, S.( 担当: 分担執筆 ,  範囲: The genus Mycoreovirus.)

    2011年  ( ISBN:9780387959184

     詳細を見る

    総ページ数:4 v.   記述言語:英語

    CiNii Books

    researchmap

  • Ninth Report of the International Committee for the Taxonomy of Viruses. A. M. Q. King et al., eds.

    Attoui, H., Mertens, P.P.C., Becnel, J., Belaganahalli, S., Bergoin, M., Brussaard, C.P., Chappell, J.D., Ciarlet, M., del Vas, M., Dermody, T.S., Dormitzer, P.R., Duncan, R., Fcang, Q., Graham, R., Guglielmi, K.M., Harding, R.M., Hillman, B., Makkay, A., Marzachì, C., Matthijnssens, J., Milne, R.G., Mohd Jaafar, F., Mori, H., Noordeloos, A.A., Omura, T., Patton, J.T., Rao, S., Maan, M., Stoltz, D., Suzuki, N., Upadhyaya, N.M., Wei, C. and Zhou, H.( 担当: 分担執筆 ,  範囲: Family Reoviridae.)

    Elsevier, Academic Press  2011年 

     詳細を見る

  • Encyclopedia of Virology 3rd Edition,

    Elsevier  2008年 

     詳細を見る

  • 作物保護の新展開ムバイオサイエンスのかけはしー

    ソフトサイエンス社  2005年 

     詳細を見る

  • Virus Taxonomy: Eighth Report of the International Committee for the Taxonomy of Viruses.

    Academic Press, San Diego.  2005年 

     詳細を見る

  • "Fungal dsRNA elements: concepts and application in agriculture, forestry and medicine."

    CRC Press. Boca Raton, Florida, USA.  2001年 

     詳細を見る

  • Virus Taxonomy: Seventh Report of the International Committee for the Taxonomy of Viruses.

    Academic Press, NY.  2000年 

     詳細を見る

▼全件表示

MISC

  • 宿主液胞型H+-ATPaseのハイジャックによるダイアンソウイルスの増殖戦略

    兵頭究, 首藤丘宇, 近藤秀樹, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022年

     詳細を見る

  • 「ヤドカリ」「ヤドヌシ」と呼ばれるウイルスの共生関係 招待

    鈴木信弘

    ネオウイルス学(河岡義裕編)集英社   2021年3月

     詳細を見る

    担当区分:責任著者  

    researchmap

  • オオムギ圃場に生息するアブラムシのウイルス叢解析

    近藤秀樹, 藤田美貴, 久野裕, 兵頭究, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021年

     詳細を見る

  • 糸状菌の新規キャプシドレスRNAウイルス2種(ポリマイコウイルスとハダカウイルス)の性状比較

    佐藤有希代, SHAMSI W., SHAMSI W., JAMAL A., BHATTI M.F., 近藤秀樹, 鈴木信弘

    日本植物病理学会報   87 ( 1 )   2021年

     詳細を見る

  • ウイルス

    鈴木信弘, 大木理, 上田一郎

    植物病理学第2版(眞山滋志・土佐幸雄編)文永堂   2020年3月

     詳細を見る

    担当区分:責任著者  

    researchmap

  • キャプシドレス11分節RNAウイルスは宿主Fusarium oxysporum株間を伝搬する際に全てのゲノム分節を維持するか?

    佐藤有希代, SHAMSI W., SHAMSI W., JAMAL A., BHATTI M. F., 近藤秀樹, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020年

     詳細を見る

  • 植物ウイルス感染の抗糸状菌植物免疫への影響

    兵頭究, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019年

     詳細を見る

  • 宿主足場タンパク質RACK1のハイジャックによる植物ウイルス増殖機構

    兵頭究, 鈴木信弘, 奥野哲郎

    日本植物病理学会報   85 ( 1 )   2019年

     詳細を見る

  • 宿主足場タンパク質RACK1は植物RNAウイルスの増殖を正に制御する

    兵頭究, 鈴木信弘, 奥野哲郎

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018年

     詳細を見る

  • Frontiers in fungal virology

    Nobuhiro Suzuki

    Journal of General Plant Pathology   83 ( 6 )   419 - 423   2017年11月

     詳細を見る

    掲載種別:書評論文,書評,文献紹介等  

    DOI: 10.1007/s10327-017-0740-9

    Scopus

    researchmap

  • 植物ラブドウイルスの分節化と進化

    近藤秀樹, 鈴木信弘

    日本進化学会大会プログラム・講演要旨集(Web)   19th   2017年

     詳細を見る

  • 温度依存的に蓄積するGrifola frondosa RNA virus1(GfRV1)がマイタケ宿主遺伝子群の発現に及ぼす影響

    小松あき子, 近藤秀樹, 佐藤真之, 鈴木信弘, 藤森文啓

    日本微生物生態学会大会(Web)   2017   ROMBUNNO.O‐019 (WEB ONLY)   2017年

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • 食用キノコの子実体形成にウイルス感染が果たす役割についての研究(3)

    小松あき子, 佐藤真之, 佐藤真之, 佐藤真之, 近藤秀樹, 西堀耕三, 鈴木信弘, 藤森文啓, 藤森文啓

    東京家政大学生活科学研究所研究報告   39   57‐61 - 61   2016年7月

     詳細を見る

    記述言語:日本語   出版者・発行元:東京家政大学生活科学研究所  

    CiNii Article

    CiNii Books

    J-GLOBAL

    researchmap

  • 活性酸素種は植物RNAウイルス増殖を正に制御する

    兵頭究, 鈴木信弘, 奥野哲郎

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016年

     詳細を見る

  • マイタケに感染するマイコウイルスの機能解析研究

    小松あき子, 佐藤真之, 近藤秀樹, 鈴木信弘, 藤森文啓

    日本分子生物学会年会プログラム・要旨集(Web)   39th   ROMBUNNO.1P‐0009 (WEB ONLY)   2016年

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Advances in Fungal Virus Research 招待 査読

    Suzuki, N

    Virus Research   219   1 - 180   2016年

     詳細を見る

    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    researchmap

  • 食用キノコの子実体形成にウイルス感染が果たす役割についての研究(2) (温故知新プロジェクト)

    小松 あき子, 佐藤 真之, 近藤 秀樹, 佐藤 真之, サトウ マサユキ, Sato Masayuki, 近藤 秀樹, コンドウ ヒデキ, Kondo Hideki, 角 真理子, スミ マリコ, Sumi Mariko, 土屋 有紀, ツチヤ ユキ, Tsuchiya Yuki, 倉橋 敦, クラハシ アツシ, Kurahashi Atsushi, 西堀 耕三, ニシボリ コウゾウ, Nishibori Kozo, 鈴木 信弘, スズキ ノブヒロ, Suzuki Nobuhiro, 藤森 文啓, フジモリ フミヒロ, Fujimori Fumihiro

    東京家政大学生活科学研究所研究報告 = Bulletin of Research Institute of Domestic Science, Tokyo Kasei University   38   79 - 84   2015年7月

     詳細を見る

    記述言語:日本語   出版者・発行元:東京家政大学生活科学研究所  

    東京家政大学生活科学研究所研究報告は、本研究所の本年度の活動成果を取りまとめたものです。本研究報告の内容の一部は、別途学会誌等に発表されることがありますのでご了承ください。

    CiNii Article

    CiNii Books

    researchmap

  • 宿主ゲノム上に存在する RNA ウイルス感染記録を紐解く. 招待

    近藤秀樹, 千葉壮太郎, 鈴木信弘

    植物感染生理談話会論文集   50   133 - 142   2015年

     詳細を見る

    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    researchmap

  • 食用キノコの子実体形成にウイルス感染が果たす役割についての研究 (温故知新プロジェクト)

    小松 あき子, 近藤 秀樹, 佐藤 真之, 近藤 秀樹, コンドウ ヒデキ, Kondo Hideki, 佐藤 真之, サトウ マサユキ, Sato Masayuki, 土屋 有紀, ツチヤ ユキ, Tsuchiya Yuki, 倉橋 敦, クラハシ アツシ, Kurahashi Atsushi, 西堀 耕三, ニシボリ コウゾウ, Nishibori Kozo, 鈴木 信弘, スズキ ノブヒロ, Suzuki Nobuhiro, 藤森 文啓, フジモリ フミヒロ, Fujimori Fumihiro

    東京家政大学生活科学研究所研究報告 = Bulletin of Research Institute of Domestic Science, Tokyo Kasei University   37   109 - 114   2014年7月

     詳細を見る

    記述言語:日本語   出版者・発行元:東京家政大学生活科学研究所  

    東京家政大学生活科学研究所研究報告は、本研究所の本年度の活動成果を取りまとめたものです。本研究報告の内容の一部は、別途学会誌等に発表されることがありますのでご了承ください。

    CiNii Article

    CiNii Books

    researchmap

  • マイタケに発見された新規RNAウイルスの性状と生物学的特性に関する研究

    角真理子, 佐藤真之, 小松あき子, 土屋(内田)有紀, 倉橋敦, 近藤秀樹, 鈴木信弘, 西堀耕三, 藤森文啓

    日本分子生物学会年会プログラム・要旨集(Web)   36th   2P-0014 (WEB ONLY)   2013年

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Hypovirus Cysteine Proteases p29 and p48

    Nobuhiro Suzuki

    Handbook of Proteolytic Enzymes   2   2192 - 2195   2013年

     詳細を見る

    記述言語:英語   出版者・発行元:Elsevier Ltd  

    DOI: 10.1016/B978-0-12-382219-2.00492-0

    Scopus

    researchmap

  • 白紋羽病菌とクリ胴枯病菌のマイコウイルス―病原力低下因子とヴァイロコントロール―農作物生産におけるウイルスの有効利用 招待

    鈴木 信弘

    JATAFF ジャーナル   1   4 - 8   2013年

     詳細を見る

    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

    researchmap

  • マイタケに存在する新規partitivirusの性状と生物学的特性に関する研究

    小松あき子, 佐藤真之, 佐藤真之, 佐藤真之, 角真理子, 土屋(内田)有紀, 倉橋敦, 近藤秀樹, 鈴木信弘, 西堀耕三, 藤森文啓, 藤森文啓

    日本分子生物学会年会プログラム・要旨集(Web)   36th   2013年

     詳細を見る

  • 植物ゲノムへ水平伝播された太古のパルティティウイルス配列

    千葉壮太郎, 近藤秀樹, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

    日本植物病理学会報   78 ( 1 )   2012年

     詳細を見る

  • 植物の核ゲノム上に見いだされるマイナス鎖RNAウイルス様配列

    近藤秀樹, 千葉壮太郎, 梅林絵里, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2012   2012年

     詳細を見る

  • Family Reoviridae. 招待

    Attoui, H, Mertens, P.P.C, Becnel, J, Belaganahalli, S, Bergoin, M, Brussaard, C.P, Chappell, J.D, Ciarlet, M, del Vas, M, Dermody, T.S, Dormitzer, P.R, Duncan, R, Fcang, Q, Graham, R, Guglielmi, K.M, Harding, R.M, Hillman, B, Makkay, A, Marzachì, C, Matthijnssens, J, Milne, R.G, Mohd Jaafar, F, Mori, H, Noordeloos, A.A, Omura, T, Patton, J.T, Rao, S, Maan, M, Stoltz, D, Suzuki, N, Upadhyaya, N.M, Wei, C, Zhou, H

    Virus Taxonomy: Ninth Report of the International Committee for the Taxonomy of Viruses.   2011年

     詳細を見る

    記述言語:英語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)   出版者・発行元:Elsevier Academic Press  

    CiNii Article

    researchmap

  • 2La13 MALDI-TOF/MSを用いたイネ種子由来Methylobacterium属細菌の分類法の確立と応用(分類・系統・遺伝学,一般講演)

    谷 明生, 奥村 麻理絵, 金原 和秀, 鈴木 信弘

    日本生物工学会大会講演要旨集   63   198 - 198   2011年

     詳細を見る

    記述言語:日本語   出版者・発行元:日本生物工学会  

    CiNii Article

    CiNii Books

    researchmap

  • 非レトロRNAウイルス由来の植物染色体配列

    近藤秀樹, 千葉壮太郎, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2011   2011年

     詳細を見る

  • 非パラレトロRNAウイルス由来の植物染色体遺伝子

    千葉壮太郎, 近藤秀樹, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

    日本ウイルス学会学術集会プログラム・抄録集   58th   2010年

     詳細を見る

  • サギソウモザイクウイルスRNAの全塩基配列の決定

    丸山和之, 近藤秀樹, 前田孚憲, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2010   2010年

     詳細を見る

  • Viral and host factors involved in symptom induction and replication of RNA viruses infecting phytopathogenic fungi.

    Salaipeth, L, Lin, Y.-H, Eusebio-Cope, A, Tanaka, T, Chiba, S, Suzuki, N

    Proceeding of the International Symposium on Fungal Genetics and Genomics   92 - 94   2009年

     詳細を見る

    記述言語:英語   掲載種別:記事・総説・解説・論説等(国際会議プロシーディングズ)  

    researchmap

  • (286) 土壌伝染性ウイルスのシステインリッチタンパク質は根のRNAサイレンシングを抑制する(平成20年度日本植物病理学会大会講演要旨)

    アンディカ イダ バクス, 近藤 秀樹, 玉田 哲男, 鈴木 信弘

    日本植物病理學會報   74 ( 3 )   243 - 243   2008年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (265) ハイポウイルスの多機能性蛋白質p29により誘発されるマイコレオウイルスのゲノム再編成(平成20年度日本植物病理学会大会講演要旨)

    鈴木 信弘, 孫 麗英

    日本植物病理學會報   74 ( 3 )   237 - 237   2008年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (266) ランえそ斑紋ウイルス(OFV)様粒子の核内での形態形成とViroplasm様構造の関連性(平成20年度日本植物病理学会大会講演要旨)

    近藤 秀樹, 野田 瑞紀, 玉田 哲男, 鈴木 信弘

    日本植物病理學會報   74 ( 3 )   237 - 238   2008年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (32)BNYVV 54 kDA : 読み過ごし領域導入植物のウイルス抵抗性を打破するBNYVV欠失変異株の出現(関西部会講演要旨,平成19年度地域部会講演要旨)

    アンディカ イダ バクス, 近藤 秀樹, 玉田 哲男, 鈴木 信弘

    日本植物病理學會報   74 ( 1 )   64 - 64   2008年2月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (31)ランえそ斑紋ウイルス構造タンパク質Mの病原性はヌクレオキャプシドタンパク質Nにより抑制される(関西部会講演要旨,平成19年度地域部会講演要旨)

    近藤 秀樹, 玉田 哲男, 鈴木 信弘

    日本植物病理學會報   74 ( 1 )   64 - 64   2008年2月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • Fungal viruses 招待

    Ghabrial, S, Suzuki, N

    Encyclopedia of Virology 3rd Edition   2   284 - 291   2008年

     詳細を見る

    記述言語:英語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)   出版者・発行元:Elsevier  

    CiNii Article

    researchmap

  • (229) RNAサイレンシング発動クリ胴枯病菌株の性格付け(平成19年度日本植物病理学会大会講演要旨)

    丸山 和之, 鈴木 信弘

    日本植物病理學會報   73 ( 3 )   236 - 236   2007年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (372) イネ萎縮ウイルスは媒介昆虫細胞間移行にPns10タンパク質から構成される管状構造体を利用する(平成18年度日本植物病理学会大会講演要旨)

    大村 敏博, 菊池 彰, 守安 裕介, 鈴木 信弘, 清水 巧, 萩原 恭二, 高橋 真実, 一木(植原) 珠樹

    日本植物病理學會報   72 ( 4 )   301   2006年11月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (370) イネ萎縮ウイルス感染細胞内におけるウイルス由来Pns4タンパク質の諸性質(平成18年度日本植物病理学会大会講演要旨)

    萩原 恭二, Wei Taiyun, 菊池 彰, 鈴木 信弘, 清水 巧, Chen Hongyan, 大村 敏博

    日本植物病理學會報   72 ( 4 )   300 - 301   2006年11月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (341) ハイポウイルスにーコードされたパパイン様プロテアーゼの更なる機能解析(平成17年度日本植物病理学会大会講演要旨)

    孫 麗英, 丸山 和之, 鈴木 信弘

    日本植物病理學會報   71 ( 3 )   276 - 276   2005年8月

     詳細を見る

    記述言語:英語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • マイコウイルス学の新展開 招待

    鈴木 信弘

    作物保護の新展開—バイオサイエンスのかけはしー(羽柴輝良編)   144 - 157   2005年

     詳細を見る

    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)   出版者・発行元:ソフトサイエンス社  

    CiNii Article

    researchmap

  • Genus Mycoreovirus 招待

    Mertens, P. P. C, Wei, C, Suzuki, N, Hillman, B. I

    Virus Taxonomy: Eighth Report of the International Committee for the Taxonomy of Viruses.   556 - 560   2005年

     詳細を見る

    記述言語:英語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

    researchmap

  • Family Hypoviridae. 招待

    Nuss, D. L, Hillman, B. I, Rigling, D, Suzuki, N

    Virus Taxonomy: Eighth Report of the International Committee for the Taxonomy of Viruses.   597 - 601   2005年

     詳細を見る

    記述言語:英語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)   出版者・発行元:Academic Press  

    CiNii Article

    researchmap

  • (309)ハイポウイルスCHV1-EP713にコードされたパパイン様プロテアーゼp29の機能領域(平成15年度日本植物病理学会大会講演要旨)

    鈴木 信弘, 丸山 和之

    日本植物病理學會報   69 ( 3 )   315 - 316   2003年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • ウイルスプロテアーゼ。

    鈴木信弘

    化学と生物 学会出版センター刊   41, 183-192. ( 5 )   335 - 344   2003年

     詳細を見る

    記述言語:日本語   出版者・発行元:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI: 10.1271/kagakutoseibutsu1962.41.335

    CiNii Article

    CiNii Books

    researchmap

  • (210)BNYVV 54k領域を導入した植物の抵抗性はRNAサイレンシングによって起こる(平成14年度 日本植物病理学会大会講演要旨)

    Andika I.B., 近藤 秀樹, 鈴木 信弘, 玉田 哲男

    日本植物病理學會報   68 ( 2 )   212 - 212   2002年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • ウイルスをもってカビを制す

    鈴木 信弘

    化学と生物 (日本農芸化学会編)   36   524 - 526   1998年

     詳細を見る

    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    researchmap

  • (311)イネ萎縮ファイトレオウイルス非構造蛋白質Pns12のリン酸化と細胞内所在(平成9年度日本植物病理学会大会講演要旨)

    鈴木 信弘, 細川 大二郎, 松浦 善治, 白子 幸男, 菊池 彰, 大村 敏博

    日本植物病理學會報   63 ( 3 )   268 - 268   1997年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (312)組み換えバキュロウイルスにより発現させたイネ萎縮ウイルスの非構造タンパク質Pns4およびPns10の昆虫細胞内における局在(平成9年度日本植物病理学会大会講演要旨)

    細川 大二郎, 鈴木 信弘

    日本植物病理學會報   63 ( 3 )   268 - 269   1997年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • Structure/function analysis of rice swarf phytoreovirus genome and its protein.

    鈴木 信弘

    Virus   47 ( 2 )   145 - 154   1997年

     詳細を見る

    記述言語:日本語   出版者・発行元:日本ウイルス学会  

    植物レオウイルスとしては初めてイネ萎縮ウイルスの全dsRNAゲノムセグメント (S1-S12) の一次構造が明らかになった。それぞれのセグメントは両末端の保存配列, 保存配列に隣接する逆反復配列をもっている。S1-S11は単一のORFを, 一方, S12は3つのORFを保有する。予想される翻訳産物が感染イネおよび感染ヨコバイ中に同定された。S1, S3, S5, S7がコア蛋白質 (P1, P3, P5, P7) を, S2, S8が外殼蛋白質 (P2, P8) を, S4, S6, S9-S12が非構造蛋白質 (Pns4, Pns6, Pns9-Pns12) をコードすることが明らかとなった。P1はRNA依存RNA合成酵素, P5はNTP結合蛋白質 (推定グアニル酸転移酵素), P7は核酸結合蛋白質であり, P1, P7は粒子中でゲノムセグメントと結合していることが明らかとなった。これらのコア蛋白質はウイルスRNAの複製に関与するものと考えられる。非構造蛋白質の機能については不明な点が多いが, Pns4が微細管封入体を構成する核酸結合蛋白質であること, Pns10が鞘状封入体蛋白質であること, Pns12が感染細胞内でリン酸化を受けること等が明らかとなった。感染性核酸-宿主細胞感染系の早急な確立が, 各ゲノムセグメントの機能分担の更なる解明に繋がるものと思われる。

    DOI: 10.2222/jsv.47.145

    CiNii Article

    researchmap

  • (15) 高発現イネ萎縮ウイルス蛋白質のSpodoptera frugiperda細胞からの精製 (東北部会)

    鈴木 信弘, 菅原 美貴子

    日本植物病理學會報   62 ( 6 )   600 - 600   1996年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (318) イネ萎縮ウイルスの非構造タンパク質のオオムギ細胞内における局在 (日本植物病理大会)

    細川 大二郎, 鈴木 信弘

    日本植物病理學會報   62 ( 3 )   339 - 339   1996年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (319) ポリシストロニックに機能するファイトレオウイルスゲノムセグメント (日本植物病理大会)

    鈴木 信弘, 菅原 美貴子, NUSS D.L., 松浦 善治

    日本植物病理學會報   62 ( 3 )   339 - 339   1996年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (291) RT-PCR法によるイネ委縮ウイルスRNAの検出 (日本植物病理大会)

    小林 淳, 近藤 尚志, 勝本 啓介, 鈴木 信弘, 宮嶌 成壽

    日本植物病理學會報   61 ( 3 )   281 - 281   1995年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (292) イネ萎縮ウイルスP5の性状解析 (日本植物病理大会)

    鈴木 信弘, 菅原 美貴子, 草野 友延, 松浦 善治, 大村 敏博

    日本植物病理學會報   61 ( 3 )   281 - 281   1995年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (28) イネ萎縮ウイルスセグメントS5にコードされた構造蛋白質 (東北部会)

    鈴木 信弘, 菅原 美貴子

    日本植物病理學會報   60 ( 6 )   763 - 763   1994年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (248) イネ萎縮ウイルスにコードされた蛋白質の植物および昆虫宿主からの定量的検出 (日本植物病理学会大会)

    鈴木 信弘, 菅原 美貴子, 草野 友延, 森 肇, 松浦 善治

    日本植物病理學會報   60 ( 3 )   382 - 383   1994年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (22) イネ萎縮ウイルス非構造蛋白質の感染イネおよび感染ヨコバイからの検出 (東北部会)

    鈴木 信弘, 菅原 美貴子, 草野 友延, 松浦 善治

    日本植物病理學會報   59 ( 6 )   763 - 764   1993年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (294) イネ萎縮ウイルスセグメントS12の in vitroでのトリシストロニックな発現 (日本植物病理大会)

    鈴木 信弘, 菅原 美貴子, 草野 友延

    日本植物病理學會報   59 ( 3 )   342 - 343   1993年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (243) イネ萎縮ウイルス由来蛋白質の保毒虫からの検出 (日本植物病理大会)

    鈴木 信弘, 森 肇, 菅原 美貴子

    日本植物病理學會報   59 ( 3 )   328 - 329   1993年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • (343) イネ萎縮ウイルス S1 の遺伝子構造解析 : 植物および動物レオウイルスのRNA依存 RNA 合成酵素間のアミノ酸配列相同性 (日本植物病理学会大会)

    鈴木 信弘, 谷村 雅子, 渡部 ゆづ子, 草野 友延, 北川 良親, 須田 成志, 工藤 博司, 上田 一郎, 四方 英四郎

    日本植物病理學會報   58 ( 4 )   630 - 631   1992年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本植物病理学会  

    CiNii Article

    CiNii Books

    researchmap

  • カボチャモザイクウイルス筒状封入体タンパク質, 不定形封入体タンパク質およびキャプシドタンパク質に対するモノクローナル抗体と他のpotyvirusの対応抗原との反応性

    鈴木 信弘, 白子 幸男, 江原 淑夫

    日本植物病理學會報   57 ( 5 )   706 - 710   1991年12月

     詳細を見る

    記述言語:英語   出版者・発行元:日本植物病理学会  

    カボチャモザイクウイルス(WMV2)筒状封入体タンパク質(CIP), 不定形封入体タンパク質(AIP)およびキャプシドタンパク質(CP)に対するモノクローナル抗体(MAb)計6種(CIP1, CIP2; AIP1, AIP2; CP1, CP2)と他のpotyvirusの対応抗原との反応性をelectroblot-ELISAを用いて調べた。各MAbは同一抗原とは強く反応したが, ズッキーニ黄斑モザイクウイルス, パパヤリングスポットウイルス(PRSV-W)の精製対応抗原およびレタスモザイクウイルス, シャガイモウイルス, カブモザイクウイルス, PRSV 5の感染葉中の対応抗原との反応性は認められなかった. 一方, ダイズモザイクウイルス(SoyMV)感染葉からは, CP2によりSoyMV CPが, AIP1およびAIP2によりSoyMV AIP様タンパク質が若干検出された。本研究により, WMV2とSoyMVの血清学的類縁関係が確認された。しかし, WMV2とSoyMV以外の他のウイルスとの血清関係については, より多くのMAbあるいはポリクローナル抗体を用いて調べる必要がある。

    CiNii Article

    CiNii Books

    researchmap

▼全件表示

講演・口頭発表等

  • From neovirology to phytopathology.

    Suzuki, N

    The first Chinese Mycovirus Symposium  2023年11月25日 

     詳細を見る

    開催年月日: 2023年11月24日 - 2023年11月27日

    記述言語:英語   会議種別:口頭発表(基調)  

    researchmap

  • Yadokari/yadonushi nature: A virus in a virus in a fungus in a plant. 招待

    Sato, Y, Suzuki, N

    The Second International Molecular Plant Protection Congress. Turkey.  2023年5月15日 

     詳細を見る

    開催年月日: 2023年5月14日 - 2023年5月21日

    記述言語:英語   会議種別:口頭発表(基調)  

    researchmap

  • Experimental and practical control measures for white root rot in fruit trees in Japan. 招待

    Suzuki, N

    Yangling International Agri-science Forum. 

     詳細を見る

    開催年月日: 2021年10月23日 - 2021年10月24日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Partnership between capsidless (+)ssRNA yadokariviruses and their host dsRNA viruses: Promiscuous or virtuous? 招待

    Suzuki, N

    Awaji International Forum on Immunity and Infection. 

     詳細を見る

    開催年月日: 2021年9月29日 - 2021年10月1日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • A neo-virus-lifestyle exhibited by a (+)RNA virus hosted by an unrelated dsRNA virus in a phytopathogenic fungus. 招待

    Suzuki, N

    The Spring Meeting of the Korean Society of Plant Pathology (KSPP 2018)  2018年4月26日 

     詳細を見る

    開催年月日: 2018年4月26日 - 2018年4月27日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Fungal RNA silencing involved in antiviral defense and RNA recombination. 招待

    Suzuki, N

    ASV 2016 Annual Meeting  2016年6月18日 

     詳細を見る

    開催年月日: 2016年6月18日 - 2016年6月22日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Antiviral defense in the phytopathogenic filamentous ascomycete, Cryphonectria parasitica. 招待

    Suzuki, N

    Swiss Society for Microbiology Annual Assembly and Meeting  2016年6月14日 

     詳細を見る

    開催年月日: 2016年6月13日 - 2016年6月16日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Another nude virus: a capsidless ssRNA virus hosted by an unrelated dsRNA virus. 招待

    Suzuki, N

    International Symposium on Virology and Fungal Genetics  2016年1月15日 

     詳細を見る

    開催年月日: 2016年1月15日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Highly activated RNA silencing via strong induction of dicer by one virus can interfere with the replication of an unrelated virus. 招待

    Chiba, S, Suzuki, N

    BMB2015 Biochemistry and Molecular Biology  2015年12月2日 

     詳細を見る

    開催年月日: 2015年12月1日 - 2015年12月4日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • A new virus lifestyle challenging the virus rules and concepts. 招待

    A new virus, lifestyle challenging the virus rules, concepts

    JICA Africa-ai-Japan Project/JSPS-AASP Sponsored Workshop on Plant Science and Resource Innovative Research Core with Pan African University.  2015年10月19日 

     詳細を見る

    開催年月日: 2015年10月19日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • From virology to plant disease control. 招待

    Suzuki, N

    JSPS Core-to-Core Program Sponsored Workshop on Crop Science and Innovation for Agriculture.  2015年10月15日 

     詳細を見る

    開催年月日: 2015年10月15日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • A new virus life style challenging the virus rules and concepts. 招待

    Zhang, R, Hisano, S, Tani, A, Kondo, H, Kanematsu, S, Suzuki, N

    13th Spanish Virology Congress  2015年6月8日 

     詳細を見る

    開催年月日: 2015年6月7日 - 2015年6月10日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Virus interference in plants and fungal hosts. 招待

    Suzuki N

    9th JKUAT Scientific and Technological Conference 2014  2014年11月13日 

     詳細を見る

    開催年月日: 2014年11月13日 - 2014年11月14日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • The chestnut blight fungus for studies on virus/host and virus/virus interactions: from a natural to a model host. 招待

    Suzuki N

    EMBO Conference Viruses of microbes: Structure and function, from molecules to communities.  2014年7月16日 

     詳細を見る

    開催年月日: 2014年7月14日 - 2014年7月18日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Dual functionality of fungal Dicer in multilayer antiviral defense. 招待

    鈴木 信弘

    International Symposium on Virus Diseases of Important Crops.  2019年9月 

     詳細を見る

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Dicer functions transcriptionally and post-transcriptionally in a multilayer antiviral defense 招待

    Suzuki, N, Andika, I. B, Kondo, H

    European Congress of Virology 2019  2019年5月 

     詳細を見る

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Another nude virus: a capsidless ssRNA virus hosted by an unrelated dsRNA virus. 招待

    Suzuki, N

    13th International dsRNA Virus Symposium  2018年9月 

     詳細を見る

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Viruses as biological control (virocontrol) agents of plant fungal pathogens. 招待

    Suzuki, N., and Kanematsu, S.

    The First International Congress of Biological Control.  2018年5月 

     詳細を見る

    記述言語:英語  

    researchmap

  • A neo-virus-lifestyle exhibited by a (+)RNA virus hosted in an unrelated dsRNA virus 招待

    N. Suzuki.

    The 16th Awaji International Forum on Infection and Immunity.  2017年9月 

     詳細を見る

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Mutualist Interactions between a Capsidless (+) ssRNA Virus and a dsRNA Virus Occurring in a Phytopathogenic Fungus. 招待

    Suzuki, N.

    ASM Microbe 2017  2017年6月 

     詳細を見る

    会議種別:口頭発表(基調)  

    researchmap

  • Virocontrol measures for phylopathogenic fungi. 招待

    Suzuki, N

    Special seminar series for phytopathology.  2023年11月23日 

     詳細を見る

    開催年月日: 2023年11月23日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Possible yadonusi/yadokari nature observed in a Japanese strain NBRC 4031 of Aspergillus foetidus.

    Fadli, M, Hisano, S, Kondo, H, Suzuki, N

    The 42nd Annual Meeting of the American Society for Virology 2023  2023年6月26日 

     詳細を見る

    開催年月日: 2023年6月24日 - 2023年6月28日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • Autophagy promotes the replication of red clover necrotic mosaic virus.

    Hyodo, K, Sudo, K, Kondo, H, Suzuki, N

    The 42nd Annual Meeting of the American Society for Virology 2020. June 24-28, 2023.  2023年6月25日 

     詳細を見る

    開催年月日: 2023年6月24日 - 2023年6月28日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • Replication of single partitiviruses in hosts across three kingdoms; Fungi, Plantae and Animalia

    Telengech, P, Hyodo, K, Ichikawa, H, Kondo, H, Suzuki, N

    The 42nd Annual Meeting of the American Society for Virology 2023  2023年6月25日 

     詳細を見る

    開催年月日: 2023年6月24日 - 2023年6月28日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • Viruses in the causal agents of dry bubble disease: Novel candidates for biological candidates for biological pest management.

    Lóránt Hatvani, Hideki Kondo, Nobuhiro Suzuki, Sándor Kocsubé, Helen Grogan

    Mushroom Days Brabanthallen 's-Hertogenbosch  2023年5月11日 

     詳細を見る

    開催年月日: 2023年5月10日 - 2023年5月12日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • Structure and assembly of fungal dsRNA viruses

    Gil-Cantero1, D, Novoa, G, Martínez-Romero, J. M, Luque, D, Nobuhiro Suzuki, N, Castón, J. R

    8th European Congress of Virology.  2023年5月5日 

     詳細を見る

    開催年月日: 2023年5月4日 - 2023年5月7日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • Mycoviruses: A novel approach for controlling mushroom pathogenic fungi.

    Hatvani, L, Kondo, H, Shahi, S, Suzuki, N, Grogan, H

    Power of Fungi and Mycotoxins in Climate Change  2022年9月16日 

     詳細を見る

    開催年月日: 2022年9月16日 - 2022年9月17日

    記述言語:英語  

    researchmap

  • A new tetra-segmented splipalmivirus with divided RdRP domains from Cryphonectria naterciae, a fungus found on chestnut and cork oak trees in Europe

    Paul Telengech, Yukiyo Sato, Sabitree Shahi, Sakae Hisano, Carolina Cornejo, Daniel Rigling, Hideki Kondo, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium  2022年6月1日 

     詳細を見る

    開催年月日: 2022年5月30日 - 2022年6月2日

    記述言語:英語  

    researchmap

  • A transfectable fusagravirus from Cryphonectria carpinicola with spherical particles

    Sakae Hisano, Subha Das, Hideki Kondo, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium.  2022年6月1日 

     詳細を見る

    開催年月日: 2022年5月30日 - 2022年6月2日

    記述言語:英語  

    researchmap

  • Fusagraviruses in Cryphonectria species

    Carolina Cornejo, Sandra Gaehwiler, Wajeeha Shamsi, Hideki Kondo, Nobuhiro Suzuki, Daniel Rigling

    The fifth International Mycovirus Symposium  2022年6月1日 

     詳細を見る

    開催年月日: 2022年5月30日 - 2022年6月2日

    記述言語:英語  

    researchmap

  • Synonymous codon usage bias in Cryphonectria parasitica viruses: Insights on evolution and host adaptation 招待

    Zeeshan Zahid, Alanna B. Cohen, Nobuhiro Suzuki, Bradley I. Hillman

    The fifth International Mycovirus Symposium  2022年6月1日 

     詳細を見る

    開催年月日: 2022年5月30日 - 2022年6月2日

    記述言語:英語  

    researchmap

  • Partnership between capsidless (+)ssRNA yadokariviruses and their host dsRNA viruses: Promiscuous or virtuous? 招待

    Yukiyo Sato, Sakae Hisano, Carlos Lopez Hererra, Hideki Kondo, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium  2022年5月31日 

     詳細を見る

    開催年月日: 2022年5月30日 - 2022年6月2日

    記述言語:英語  

    researchmap

  • Low-level IRES activity likely contributes to the replication restriction of Cryphonectria hypovirus 1 in Cryphonectria carpinicola 招待

    Sabitree Shahi, Sotaro Chiba, Yoshihiro Takaki, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium  2022年5月31日 

     詳細を見る

    開催年月日: 2022年5月30日 - 2022年6月2日

    記述言語:英語  

    researchmap

  • Argonaute-independent antiviral RNA interference in the chestnut blight fungus, Cryphonectria parasitica.

    Yukiyo Sato, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium. Gargnano,  2022年5月31日 

     詳細を見る

    開催年月日: 2022年5月30日 - 2022年6月2日

    記述言語:英語  

    researchmap

  • Diverse partitiviruses from the phytopathogenic fungus, Rosellinia necatrix.

    Telengech, P, Hisano, S, Micheni, C. M, Hyodo, K, Arjona-Lopez, J.M, Lopez-Herrera, C, Kanematsu, S, Kondo, H, Suzuki, N

    The 40th Annual Meeting of the American Society for Virology 2020. 

     詳細を見る

    開催年月日: 2021年7月19日 - 2021年7月23日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • Partnership between capsidless (+)ssRNA yadokariviruses and their host dsRNA viruses: Promiscuous or virtuous?

    Yukiyo Sato, Sakae Hisano, Subha Das, Carlos Lopez Hererra, Hideki Kondo, Nobuhiro Suzuki

    The 40th Annual Meeting of the American Society for Virology 2020. 

     詳細を見る

    開催年月日: 2021年7月19日 - 2021年7月23日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • Prevalence, diversity and impact of mycoviruses infecting plant pathogenic fungi pf rice in the Philippines.

    Guinto, T, Cope, A, Urzo, M, Budot, B, Yanoria, M. J, Jonson, G, Oliva, R, Kondo, H, Suzuki, N

    Philippine Phytopathological Society Pest Management Council of the Philippines, Inc. PMCP 2021 

     詳細を見る

    開催年月日: 2021年7月6日 - 2021年7月7日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • In-tree behavior of diverse viruses infecting the chestnut blight fungus.

    Suzuki, N, Aulia, A, Shahi, S, Hillman, B. I, Cornerjo, C, Rigling, D

    The 39th Annual Meeting of the American Society for Virology 2020. 

     詳細を見る

    開催年月日: 2020年6月13日 - 2020年6月17日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • 2A-mediated co-translational cleavage is prerequisite for the viability of yado-kari virus 1 hosted by yado-nushi virus 1.

    Das, S, Alam, M, Zhang, R, Hisano, S, Sato, Y, Kondo, H, Suzuki, N

    The 39th Annual Meeting of the American Society for Virology 2020 

     詳細を見る

    開催年月日: 2020年6月13日 - 2020年6月17日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • Maintenance and loss of dispensable genomic segments of a polymyco-like capsidless fungal virus with an eleven-segmented RNA genome during its infection cycle.

    Sato, Y, Shamsi, W, Jamal3, A, Bhatti, M. F, Kondo, H, Suzuki, N

    The 39th Annual Meeting of the American Society for Virology 2020 

     詳細を見る

    開催年月日: 2020年6月13日 - 2020年6月17日

    記述言語:英語   会議種別:口頭発表(一般)  

    researchmap

  • Vigorous induction of dicer-like 2 gene by a virus interferes with an unrelated virus in the model phytopathogenic fungus Cryphonectria parasitica. 招待

    Chiba, S, Suzuki, N

    12th PSJ Plant Virus Disease Workshop:The Interface between plant and fungal viruses II.  2016年3月14日 

     詳細を見る

    開催年月日: 2016年3月14日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • A new virus life style challenging the virus rules and concepts. 招待

    R. Zhang, S. Hisano, A. Tani, H. Kondo, S. Kanematsu, N. Suzuki

    63rd Annual Meeting of the Japanese Society for Virology  2015年11月23日 

     詳細を見る

    開催年月日: 2015年11月22日 - 2015年11月24日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

  • Mycoviruses in C. parasitica and their biological control potential. 招待

    鈴木 信弘

    WSL Colloquium.  2019年10月30日 

     詳細を見る

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    researchmap

▼全件表示

受賞

  • 山陽新聞賞(学術功労)

    2024年1月   山陽新聞社  

     詳細を見る

  • Top Peer Reviewer Award

    2019年9月   Publon (Web of Science Group)  

     詳細を見る

  • 学会賞

    2017年3月   日本植物病理学会  

    鈴木 信弘

     詳細を見る

  • 日本ウイルス学会奨励賞

    1997年  

     詳細を見る

    受賞国:日本国

    researchmap

  • 日本植物病理学会奨励賞

    1995年  

     詳細を見る

    受賞国:日本国

    researchmap

共同研究・競争的資金等の研究

  • Investigation into the potential role of fungal viruses in the dry bubble disease of cultivated mushroom – towards biological pest management

    研究課題/領域番号:Leca-VIR  2022年04月 - 2023年10月

    European Commission (EU) Research Leaders 2025/Marie Skłodowska Curie Fellowship (2022-2025) 

      詳細を見る

    担当区分:連携研究者 

    researchmap

  • マイコイミュニティ研究の最前線とその植物病理学への新展開

    研究課題/領域番号:21H05035  2021年07月 - 2026年03月

    日本学術振興会  科学研究費助成事業 基盤研究(S)  基盤研究(S)

    鈴木 信弘

      詳細を見る

    配分額:188240000円 ( 直接経費:144800000円 、 間接経費:43440000円 )

    researchmap

  • 植物・菌・動物の生物界を跨いだウイルス感染:宿主との攻防と適応戦略

    研究課題/領域番号:21K18222  2021年07月 - 2025年03月

    日本学術振興会  科学研究費助成事業 挑戦的研究(開拓)  挑戦的研究(開拓)

    鈴木 信弘

      詳細を見る

    配分額:25870000円 ( 直接経費:19900000円 、 間接経費:5970000円 )

    researchmap

  • 糸状菌ウイルスのネオ・ライフスタイル

    研究課題/領域番号:16H06436  2016年06月 - 2021年03月

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    鈴木 信弘, 近藤 秀樹

      詳細を見る

    配分額:117520000円 ( 直接経費:90400000円 、 間接経費:27120000円 )

    柱1「裸性を持つであろうポリマイコウイルスの3者生態系における役割の解析」パキスタン産ポリマイコ様ウイルス(HadV1)感染、非感染菌株でフェノーム解析を行った。まず、極めてユニークなHadV1のゲノム配列を決定した。11本の(+)RNA分節を持つことが示され、ポリマイコウイルスに保存されている富PAS(プロリンーアラニンーセリン)タンパク質がHadV1には保存されていないことが判明した。次にHadV1感染Fusarium oxysporumから単胞子分離により、非感染株を複数得た。ウイルス感染株、非感染株を比較したが、ウイルスによる生物学的な性状への影響を観察することができなかった。また、ウイルス実態を調べるた結果、HadV1は、既報のポリマイコウイルスあるいはハイポウイルスとは分画パターンを示し、通常それらのウイルスが沈降する条件での超遠心では沈降しないことが明らかとなり、新規のライフスタイルを持つキャプシドレスウイルスと示唆された。
    柱2「ヤドカリ・ヤドヌシウイルスの4者生態系での役割の解析」 では、YkV1感染性クローンを利用した逆遺伝学とYnV1トランスフェクション法を用いて詳細に解析した。その結果、YkV1の複製にはYkV1 RdRpが必須でそれがポリプロテインから切断されることが必須であることが示された。
    柱3「ネオ・ライフスタイルを持つウイルスの探索と生態学的役割の考察」 では、スペイン産、フィリピン産およびバングラデシュ産植物感染性糸状菌の提供受け、網羅的にウイルス探索を行った。その結果、スペイン産からYkV1と配列類似性を示す複数のウイルス、YkV2, YkV3, YkV4a, YkV4bが見つかったが、YnV1と配列類似性を示すウイルスは見つからなかった。YkV4のパートナーはメガトティウイルスであるこが、示唆された。

    researchmap

  • 植物病原糸状菌の抗ウイルス自然免疫機構

    研究課題/領域番号:25252011  2013年04月 - 2017年03月

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    鈴木 信弘, 中屋敷 均, 近藤 秀樹

      詳細を見る

    配分額:45760000円 ( 直接経費:35200000円 、 間接経費:10560000円 )

    本研究では、「RNA干渉機構(RNAi)」による菌類ウイルスに対する自然免疫機構(複製阻害)を植物病原糸状菌で解析し、ウイルス防御機構の研究における植物・動物界に次ぐ第三極の形成を目指した。その結果、予想しなかった大きな成果が得られた。AGOを必要としないウイルス防御、AGOは本来細胞レベルウイルス防御に関わるRNAiの鍵因子であるが、RNAiに対して抑制的に働くAGOの発見、ウイルスのパターン認識からRNAi誘導に関与する宿主因子探索のスクリーニング系の構築等、の成果は抗ウイルスRNAiのパラダイムシフトをもたらす可能性が非常に高い。上記の成果は、一流専門誌あるいは一般誌に公表した。

    researchmap

  • 絶対寄生菌ウイルスの研究プラットフォーム構築と生物防除への挑戦

    研究課題/領域番号:23K18029  2023年06月 - 2026年03月

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

    近藤 秀樹, 鈴木 信弘

      詳細を見る

    担当区分:研究分担者 

    配分額:6500000円 ( 直接経費:5000000円 、 間接経費:1500000円 )

    researchmap

  • 作物超個体における根圏RNAウイルス叢の実体解明とその生態学的役割

    研究課題/領域番号:23H02214  2023年04月 - 2027年03月

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    近藤 秀樹, 久野 裕, 兵頭 究, 鈴木 信弘

      詳細を見る

    担当区分:研究分担者 

    配分額:18590000円 ( 直接経費:14300000円 、 間接経費:4290000円 )

    researchmap

  • 3つの生物界を跨いだウイルス感染

    研究課題/領域番号:22F22095  2022年07月 - 2025年03月

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, TELENGECH PAUL

      詳細を見る

    配分額:2300000円 ( 直接経費:2300000円 )

    researchmap

  • クリ樹体内での多様なウイルス(感染クリ胴枯病菌)の挙動と生物防除能

    研究課題/領域番号:JPJSBP120  2022年04月 - 2024年03月

    日本学術振興会  二国間交流事業 オープンパートナーシップ共同研究 

      詳細を見る

    担当区分:研究代表者 

    researchmap

  • モデル糸状菌アカパンカビを用いたウイルス研究フロンティア

    研究課題/領域番号:21K19086  2021年07月 - 2023年03月

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

    近藤 秀樹, 鈴木 信弘, 本田 信治

      詳細を見る

    配分額:6500000円 ( 直接経費:5000000円 、 間接経費:1500000円 )

    本課題では、菌類ウイルス学のモデル実験系を構築するため、代表的な実験系糸状菌の一種であるアカパンカビを用いてウイルス学を展開するための基盤整備を進めている。以下に本年度の研究実績を示す。
    柱① アカパンカビの感染性ウイルスを用いたウイルス学:先の報告で見出されたアカパンカビに自然感染する新規ウイルスの性状解析を進を進めている(Hondaら, Nature Comm 2020、未発表)。これまでに、Neurospora crassaのNGSデータでデルタフレキシ及び新奇RNAウイルス(配列断片)が見出された菌株をついて、感染ウイルスのゲノム配列解析を進めている。さらに、Neurospora intermediaのNGS解析で見出されたウイルス様コンテイグ(フザリウイルス、ミトウイルス、デルタフレキシウイルス)について、元菌株のRT-PCRによりその存在確認と部分配列の決定をおこなった。一部ウイルスについては、3'RLM-RACEによる末端配列を進めている。これらのウイルスのRdRP配列に基づく分子系統樹(最尤法)を作成し、系統学的位置付けを検討した。
    柱② ウイルス病徴発現・複製、防御機構/ウイルス反撃に関与する因子の同定と機構解明:N. crassaの抗ウイルスRNAi機構の鍵酵素であるdcl1, dcl2 qde2の単独欠損変異株(dcl1、dcl2, qde2)、二重欠損変異株(dcl1/dcl2, qde2/dcl1, qde2/dcl2)、三重欠損変異体(qde2/dcl1/dcl2)および野生型を用いて、異なるゲノムタイプの二種マイコウイルス(一本鎖RNAウイルスと分節型dsRNAウイルス)の混合感染株をそれぞれ作出した。これらの混合感染株について全RNAを抽出し、小分子RNA分画のRNA-seq解析を行った。現在,得られたRNA-seqの配列データの詳細解析を進めている。

    researchmap

  • マイコイミュニティ研究の最前線とその植物病理学への新展開

    研究課題/領域番号:21H04727  2021年04月 - 2025年03月

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    鈴木 信弘, 近藤 秀樹, 兵頭 究

      詳細を見る

    配分額:41600000円 ( 直接経費:32000000円 、 間接経費:9600000円 )

    researchmap

  • 宿主アカパンカビの抗ウイルス免疫暴走化機序の解明

    研究課題/領域番号:21H02153  2021年04月 - 2024年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    本田 信治, 鈴木 信弘

      詳細を見る

    配分額:17420000円 ( 直接経費:13400000円 、 間接経費:4020000円 )

    本研究代表らは構築したモデル糸状菌アカパンカビのウイルス感染系(Honda et al, Nature Comm, 2020)を用いて、宿主がウイルス感染に対して過剰に免疫応答することで、病気になることを発見した。本研究の目的は、この免疫暴走化に関わる「ウイルス認識機構とその下流因子」、免疫暴走の鍵となる「病的原因遺伝子」を同定し、その分子機序を解明することである。
    これまでの解析により、アカパンカビのDICERであるDCL-2を欠損させると、免疫暴走化が抑制されて病気から回復することを見出している。この結果から、DCL-2が有するdsRNA結合部位がウイルスのゲノムdsRNAを認識し、下流の免疫暴走の鍵となる「病的原因遺伝子」の転写を活性化すると考えた。そこで、研究計画に沿って種々の機能変異DCL-2を有する形質変換株を作製し、解析を行った。その結果、DCL-2のdsRNA結合部位、及び、DICER活性が免疫暴走化に関与することを確認した。
    また、クリ胴枯病菌ではSAGA複合体がウイルス応答遺伝子群の転写活性化に関与していることが報告されていることから(Andika et al, PNAS, 2017)、SAGA複合体を介する転写活性化と免疫暴走化の関係を調査した。その結果、DCL-2欠損と同様に、SAGA複合体の構成因子を欠損させると、免疫暴走化が抑制されることから、アカパンカビとクリ胴枯病菌に共通するウイルス応答機構の存在が示唆された。

    researchmap

  • クリ胴枯病ヴァイロコントロール因子の宿主範囲決定因子

    研究課題/領域番号:21F21093  2021年04月 - 2023年03月

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, SHAHI SABITREE

      詳細を見る

    配分額:2300000円 ( 直接経費:2300000円 )

    researchmap

  • ネオウイルス学:生命の源流から超個体、そしてエコ・スフィアーへ

    研究課題/領域番号:21H00456  2021年04月 - 2022年03月

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    河岡 義裕, 朝長 啓造, 澤 洋文, 松浦 善治, 川口 寧, 渡辺 登喜子, 鈴木 信弘, 高橋 英樹, 長崎 慶三

      詳細を見る

    配分額:3900000円 ( 直接経費:3000000円 、 間接経費:900000円 )

    「ネオウイルス学」領域内の計画研究および公募研究で得られた各班それぞれの研究テーマについて研究成果を取りまとめ、さらに班間、あるいは、「共進化」、「共生」、「多様性」ユニット間の連携テーマの重要性・意義を考慮しつつ、領域全体の研究成果を取りまとめた。業績集を作成し、冊子体として印刷して領域内外で配布することによって、本領域の研究成果を発信した。報告内容は、出来るだけ分かりやすい記述を心がけ、学術分野だけでなく、一般国民にも読んでもらえるような業績集を作成した。また、高校生、大学生、一般の国民に向けた講演会やセミナー、そして領域ホームページとTwitterによって、領域の活動内容や研究成果を報告することによって、国民に向けた研究成果の発信に努めた。また、新しい試みとして「ウイルスおりがみ」を作成した。ウイルスおりがみは、おりがみを折ることで、一般国民にウイルスの面白さを知ってもらうことを目的とした、科学コミュニケーションツールであり、公募研究班の牧野晶子助教(京都大)、デザイン・ユニットCOCHAE、合同会社SHIBUYA PUBLISHING & BOOKSELLERS、三宅文子アート・プランナーらのグループによって作成された。ウイルスおりがみには領域内の研究者による解説も加えられており、ウイルスの基本、構造の面白さ、役に立つウイルス、生き物の中にいる多様なウイルスについて学ぶことができる。ウイルスおりがみの配布の公募をしたところ2288部の応募があり、抽選で500部を無償で配布した。日本デザイン振興会が主催するグッドデザイン賞へウイルスおりがみを出品中である。

    researchmap

  • 作物根圏におけるウイルス叢の多様性とその感染動態から紐解く生態的意義

    研究課題/領域番号:20H02987  2020年04月 - 2023年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    近藤 秀樹, 久野 裕, 鈴木 信弘, 兵頭 究

      詳細を見る

    配分額:17680000円 ( 直接経費:13600000円 、 間接経費:4080000円 )

    本課題では、ムギ類生存圏(特に根系)におけるウイルス叢の多様性・普遍性や動態を紐解き、さらにそれらの未知なる生態学的役割の一端に迫ることを目指している。以下に本年度の研究実績を示す。
    柱①根圏に存在する主要な菌類・昆虫ウイルス群の宿主探索:初年度に引き続き、ムギ類(オオムギ・コムギ)の地上部・根系、さらにアブラムシ、うどんこ病菌などのRNAseqデータを取得した。得られたデータセットはウイルス配列の確認作業や分子系統解析を進めている。さらに、北海道のコムギサンプルより見出された新規フレキシウイルス(WVQ)の解析の結果,当該圃場に三系統が存在し、ライムギにも感染できること、さらに土壌伝染性ウイルスの可能性が高いことを見出している。根系ウイルス叢のリザーバー探索については、本年度はオオムギ根のRNAseqデータに含まれる微生物リードの抽出と配列相同性解析により、微生物叢の概要を調査した。
    柱②根圏ウイルスの生物界を跨ぐ水平伝搬ポテンシャルと宿主への影響:昨年度より継続中のルテオウイルスなど新規植物ウイルスのゲノム解析を進めており、RT-PCRおよびRLM-RACE解析により全長ゲノムRNA配列を確認・決定した。また、圃場のうどんこ病菌のウイルスについては、今年度はdsRNAを鋳型にした全長cDNAのPCR増幅法の検討を進め、そのアンプリコン配列解析により未同定のゲノム末端配列の解析を進めている。
    柱③根圏ウイルス叢の年次変動とムギ類ウイルスの病原性の(再)評価:核酸抽出を伴わない簡易ウイルス診断法(RT-PCRをベースとする)の条件を設定し、一部圃場サンプル(葉)でルテオウイルス等の検出を進めている。現在、圃場オオムギサンプル(保存している過年度のを含む)について網羅的に検定すすめている。また、アブラムシ媒介性新規植物ウイルスについては、チャンバー内試験にて病原性評価を進めている。

    researchmap

  • ヤドカリウイルスの新奇生活スタイル(宿借性)の証明

    研究課題/領域番号:18F18086  2018年04月 - 2020年03月

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, DAS SUBHA

      詳細を見る

    配分額:2300000円 ( 直接経費:2300000円 )

    果樹白紋羽病菌のウイルスハンティングの過程で、ユニークなウイルス/ウイルス間相互作用を発見した。本菌から得られるウイルス精製画分からは、ヤドヌシウイルス(YnV1), ヤドカリウイルス(YkV1)が検出された。YnV1は非分節型dsRNAウイルスで、 キャプシド蛋白質(CP)とRNA合 成酵素(RdRp)をコードする。YkV1は RdRpドメインとプロテアーゼドメインをコードするプラス(+ )1本鎖RNAウイルスである。
    具体的に下記の項目を実施し、「ヤドカリウイルスYkV1の宿借性]のコンセプトの確立に努めた。
    1) 昨年作成したYkV1 RdRp GDDモティーフの変異株をさらに増やし、同様の解析を進め、「宿借性」にYkV1 RdRpが必須であることを確認した。
    2) 昨年合成した6つの2A様プロテアーゼ変異株に加え、さらに6つの変異をYkV1感染性cDNA上で変異を導入し、感染性の有無とプロテアーゼ活性を調べた。その結果、バキュロウイルスを用いた昆虫細胞内でのプロテアーゼ活性と宿主菌でのウイルス複製能が一致した。すなわち、プロテアーゼ活性を消失した変異株は、白紋羽病菌での複製能も消失した。興味深いことに、弱いプロテアーゼ活性を有する変異株は、長期間維持すると2A様プロテアーゼが野生型に復帰したウイルス株が出現した。以上の結果は、YkV1の複製には、RdRp領域がポリプロテインから切断される必要があることを強く示唆する。
    3) YkV1/YnV1感染株から塩化セシウム平衡密度勾配遠心法により、粒子を分画した結果、それぞれ単独で粒子化されていることを示唆するデータを得た。

    researchmap

  • 植物病原糸状菌の抗ウイルス免疫機構研究のフロンティア

    研究課題/領域番号:17H01463  2017年04月 - 2021年03月

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    鈴木 信弘, 近藤 秀樹, 兵頭 究

      詳細を見る

    配分額:41990000円 ( 直接経費:32300000円 、 間接経費:9690000円 )

    1ウイルス由来RNAi抑制蛋白質の作用点の解明を引き続き進めた。GFP蛍光タンパク質をレポーターとして、菌類で機能するRNAi抑制タンパク質の評価法を開発した(Aulia et al., 2020) 。本手法を用いて、ハイポウイルス4(CHV4)にRNAi抑制があること、さらにその活性はシステインプロテアーゼp24にマップされることを明らかにした。このp24の自己切断箇所を生化学的に同定し、p24がすでに同定済みのCHV1 p29同様「dcl2, agl2の転写誘導阻害」に関与することを証明した(Aulia et al., 投稿準備中)。
    p29については、GFP蛍光タンパク質遺伝子をp29遺伝子中央に挿入することで、機能が欠損しないことが判明した。そこで、タグ標識p29を用いて「p29に相互作用する宿主蛋白質の網羅探索」のために免疫沈降試験を行い、相互作用タンパク質の質量分析を行なっている。
    2新規ウイルス防御機構、新規ウイルスによるRNAiへの反撃機構の解析も試みた。1)菌類宿主のDicerは、他の真核生物と同様にウイルスdsRNAの切断を担うが (Chiba & Suzuki, PNAS 2015)、宿主菌の多数の遺伝子の転写亢進にも必須である (Andika et al., PNAS 2017)、2)その宿主菌遺伝子の中には、Dicer遺伝子自身(フィードバック機構)、ウイルス病徴の軽減につながる遺伝子も含まれる、3)1)の転写調節には真核生物に広く保存されているSAGA複合体とダイサーが必須であることを示した (Andika et al., PNAS 2019)。これは、ウイルス複製阻害(RNAi)と転写調節を伴う病徴軽減(第三の抗ウイルス機構)の少なくとも2層のウイルス防御にダイサーが関与する(デュアル機能)ことを示すブレイクスルーである。

    researchmap

  • ネオウイルス学:生命の源流から超個体、そしてエコ・スフィアーへ

    研究課題/領域番号:16H06429  2016年06月 - 2021年03月

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    河岡 義裕, 朝長 啓造, 澤 洋文, 松浦 善治, 川口 寧, 渡辺 登喜子, 鈴木 信弘, 高橋 英樹, 長崎 慶三, 川野 秀一

      詳細を見る

    配分額:122980000円 ( 直接経費:94600000円 、 間接経費:28380000円 )

    (1)計画・公募研究の推進:計画・公募研究を、研究活動支援システム、領域班会議などを通じて積極的にサポートし、計画研究とともにネオウイルス学創成に目がけた研究を推進している。高度情報処理支援として、計画研究班と公募研究班に対して、スーパーコンピュータシステム・シロカネの高速処理体制を構築するとともに、データ解析プログラムの連携・共有、コミュニケーションツールslackを用いたシロカネと解析プログラムの情報共有を行った。
    (2)領域班会議の開催:2019年度は、6月10~12日に兵庫県淡路島において第6回領域班会議、また 11月6日~8日に兵庫県淡路島において、第7回領域班会議を行い、 各計画・公募研究班による未発表データを含めた進捗報告を行った。会議では、活発な議論が展開され、共同研究や技術提供が活性化されるとともに、領域内の研究者間の有機的な連携が強化された。また今年度は、 テレビ会議システムを用いて、月に一度の定例会を行い、各計画・公募研究班の研究進捗の報告などを行なうことによって、領域全体の研究の推進を図った。また領域内でのトレーニングコース(ドライ解析の講習会など)を3回行った。
    (3)広報活動:本領域の研究活動を国民に広く発信するため、ホームページ/フェイスブックページ/ツイッターにおいて、2019年度は27/18/61件の記事を掲載した。フォロワー数は2018年度と比較して、フェイスブックページは206から287名、ツイッターは239から452名に増加した。また2019年度は、領域の研究内容の概説を掲載したニュースレターを2回発行し、また11回のアウトリーチ活動を行なった。

    researchmap

  • 「ネオウイルス学」の国際活動支援

    研究課題/領域番号:16K21723  2016年06月 - 2021年03月

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    河岡 義裕, 朝長 啓造, 澤 洋文, 高橋 英樹, 川口 寧, 渡辺 登喜子, 松浦 善治, 鈴木 信弘, 長崎 慶三

      詳細を見る

    配分額:46800000円 ( 直接経費:36000000円 、 間接経費:10800000円 )

    国際活動支援班では、各研究班の海外における研究活動を促進するために、 (1) 国際研究ネットワークの整備および強化、(2) 円滑な海外での研究試料の採取支援・提供、(3) 国際シンポジウムの開催および海外研究者の招聘による人的連携の促進、(4) サンプル収集のための海外への研究者の派遣の支援、(5) 最先端研究を行う先進諸国への若手研究者の中長期的派遣の支援、(6) 国際研究者ネットワークへの積極的な情報発信を行なっている。
    2019年度は、国内で国際学会を4回 [Thermophiles国際学会、第18回あわじ感染と免疫国際フォーラム、日本ウイルス学会学術集会、Asian Mycological Congress 2019]、また、ワークショップを3回 [ウイルス情報解析ワークショップ、Thermophiles Workshop、ネオウイルス学ワークショップ]を共催して、海外から総計10名の研究者を招聘した。招聘した研究者と計画研究班、および公募研究班班員は学会後も共同研究を継続しており、人的連携を構築した。
    また、南米のボリビア共和国に生息する節足動物の採集とウイルス調査を目的として、計画研究班の若手研究者を2名派遣して、サンプルの収集を実施させた。本活動には、計画研究班からさらに2名の研究者も参加して、蚊、ダニを採集した。また、本活動によるガブリエル・レネ・モレノ国立自治大学のJuan Antonio Pereira博士との共同研究に基づいて、計画研究班の若手研究者の研究室に所属する大学院生が、文部科学省の官民協働海外留学創出プロジェクト「トビタテ!留学JAPAN」を活用し、ガブリエル・レネ・モレノ国立自治大学に短期留学を実施し、現地での共同研究を推進した。
    2018年度に引き続き、本領域のホームページの英語版を通して国際社会に本領域の活動を発信している。

    researchmap

  • 新ウイルスライフスタイル:dsRNAウイルスを宿主とする裸のssRNAウイルス

    研究課題/領域番号:15F15391  2015年11月 - 2018年03月

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, ALAM MD.

      詳細を見る

    配分額:2300000円 ( 直接経費:2300000円 )

    [yado-kari virus 1のyado-nushi virus1への複製依存性と宿主との相互作用]
    5) 粒子トランスフェクション法によるYnV1単独感染株、混合感染株を作出した。YnV1には3つの変異型が確認されており、それぞれについて単独感染株、混合感染株を作製することに成功した。6) YkV1感染性完全長cDNAの作製し、YnV1単独感染株に導入することでYkV1の「ヤドカリ性」を証明する(RNA Virophageの証明)。7) 感染性クローンが得られたので、各種変異株(RdRpモティーフ、ポリプロテイン切断部位等)を作成し、それらのYkV1複製能への影響を調べた。その結果、RNA依存RNA合成酵素の触媒残基GDDの欠失あるいは置換変異株ではYkV1の複製能は消失した。また、2A-likeプロテアーゼの触媒残基の置換変異株でも同様の傾向が認められた。これは非常に大きな成果と言える。8) 白紋羽病菌と分類上目が異なるクリ胴枯病菌(モデル糸状菌宿主)での複製能を調べた。これまでのところ、陰性の結果が得られた。クリ胴枯病菌の免疫不全株で試したが、陰性であった。

    researchmap

  • メタゲノム解析に基づく菌類ウイルス叢の解明と有効利用に関する研究

    研究課題/領域番号:15K07312  2015年04月 - 2018年03月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    近藤 秀樹, 鈴木 信弘

      詳細を見る

    配分額:5070000円 ( 直接経費:3900000円 、 間接経費:1170000円 )

    菌類ウイルスの同定はウイルスの多様性や進化の理解に大きく寄与するが,うどんこ病菌(子嚢菌類)やさび病菌(担子菌類)のような活物寄生菌ではウイルスの存在は長らく不明であった。 本課題では、赤クローバーうどんこ病菌から二本鎖RNAを取得し,次世代シークエンサーによりウイルス叢解析を行った。その結果,赤クローバーうどんこ病菌に9種以上の新規トティウイルスの存在が明らかになった。さらに,類似するトティウイルス様配列をマメ科のさび病菌の転写物ライブラリーに見いだした. これらの成果は,トティウイルスがうどんこ病菌とさび病菌の間を嘗て水平伝搬し,それぞれの宿主と共進化したとことを示唆している.

    researchmap

  • ミトコンドリア感染性ウイルスの感染機構の解明と有効利用

    研究課題/領域番号:15K14663  2015年04月 - 2018年03月

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    鈴木 信弘

      詳細を見る

    配分額:4030000円 ( 直接経費:3100000円 、 間接経費:930000円 )

    ミトウイルスは菌類を宿主とし1本鎖(ss)RNAゲノムを持つ唯一無二のミトコンドリア感染性ウイルスである。このうち、Cryphonetria parasitica mitovirus 1(CpMV1)は世界3大樹病の病原であるクリ胴枯菌(子のう菌)に感染し、複雑な3者間相互作用(クリ樹-子のう菌、子のう菌-ウイルス:2つの宿主・パラサイトの関係が存在)を織りなす。本研究は、このユニークなウイルスの感染機構(宿主範囲、水平伝染搬能、宿主防御機構への対抗手段、病徴発現機構などのウイルス学的命題)の解明した世界最初の例としてなる。

    researchmap

  • 白紋羽病菌のヴァイロコントロールに向けた新規2分節dsRNAの性状解析

    研究課題/領域番号:13F03083  2013年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, JAMAL Atif

      詳細を見る

    配分額:2300000円 ( 直接経費:2300000円 )

    白紋羽菌、Rosellinia necatrixは日本の果樹に重大な被害を齎している植物糸状菌病である。本研究では、白紋羽菌に感染し、その病原力を衰退させる新規ウイルス(Rosellinia necatrix megabirnavirus 1, RnMBV1)のウイルス学的性状とヴァイロコントロール(生物防除)因子としての潜在力を調べ、マイコウイルス学、ヴァイロコントールへの貢献を目指し、以下の成果を記述する。1-1ウイルス粒子トランスフェクション法を利用し、菌細胞質不和合性の複数の白紋羽病菌への感染性ならびに病原力低下効果を確認した。
    1-2 RnMBV1を目が異なるクリ胴枯病菌への感染性ならびに病原力低下効果を確認した。RnMBV1のヴァイロコントロール因子としての可能性を広げる成果である(JAMAL氏が来日する以前に前倒しで実施し、論文掲載にこぎ着けた)。
    1-3ヴァイロコントロール因子の長期安定保存が不可欠であるが、精製RnMBV1粒子は -80Cで1年以上安定に感染性を保持していた。 2-1 RnMBV1ゲノムセグメントはそれぞれ極めて長い(約1.6 kb)の非翻訳領域(ミニシストロンを複数含む)と2つのORFをもつ。5’側ORFがどのような機構で完全長mRNAから翻訳されるかを、リポーター系を確立した。
    2-2 5’非翻訳領域がIRES(内部リボゾームエントリー部位)活性を持つことを確認し、さらにそのコア活性領域をORF1領域近傍の500塩基の絞り込みに成功した。
    2-3感染性RnMBV1核酸の合成を試みたが、成功には至らなかった。

    researchmap

  • 植物ゲノムに存在する非レトロウイルス様配列の挿入メカニズムと病理学的意義

    研究課題/領域番号:24580064  2012年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    近藤 秀樹, 鈴木 信弘, 丸山 和之

      詳細を見る

    配分額:5590000円 ( 直接経費:4300000円 、 間接経費:1290000円 )

    近年,哺乳動物などの核ゲノムに非レトロRNAウイルス様配列(NRVS)が発見され,それらは古のウイルス感染記録と考えられている. 本研究では,植物,菌類,昆虫の核ゲノムで新規NRVSを探索し,配列解析を行った.その結果,プラス鎖RNAウイルスであるベニウイルスの感染記録を植物(ヒヨコマメ)と昆虫(サシガメ)の核ゲノムに見出した.加えて,菌類ではじめてとなるマイナス鎖RNAウイルスの感染記録をうどんこ病菌核ゲノムに発見した.これらの成果はRNAウイルスの起源や進化を考える上で興味深い情報を提供する.さらに,本研究ではこれらのNRSVの挿入メカニズムと生物学的な存在意義について議論を加えた.

    researchmap

  • アカパンカビモデル糸状菌を用いたウイルス/宿主相互作用解析系の確立

    研究課題/領域番号:24658041  2012年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    鈴木 信弘

      詳細を見る

    配分額:4160000円 ( 直接経費:3200000円 、 間接経費:960000円 )

    ウイルス感染の報告例がないアカパンカビ(Neurospora crassa, 子のう菌、糸状菌)で複製可能なウイルスを探索し、ウイルス/宿主相互作用解析のモデル系を確立するのが目的である。クリ胴枯病菌感染性マイコレオウイルス(MyRV1)、白紋羽病菌感染性パルティティウイルス(RnPV2)をアカパンカビの野生型、RNAサイレンシング成分(agl, dcl, rdr)各種変異株菌に導入し、ウイルス複製を確認した。それら宿主成分のウイルス感染への影響を解析中である。今後、アカパンカビの強力な遺伝学、さらには整備された各種研究ツールを利用し、宿主因子の探索を進め、さらに詳細な解析を行う予定である。

    researchmap

  • ハイポウイルスの多機能性蛋白質p29により誘導されるレオウイルスゲノム再編成

    研究課題/領域番号:11F01085  2011年 - 2013年

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, COPE A.E.

      詳細を見る

    配分額:1500000円 ( 直接経費:1500000円 )

    クリ胴枯病菌(Cryphonectria parasitica)は子のう菌の一種でクリ樹を枯死させる植物病原菌である。クリ胴枯病菌は少なくとも5科のメンバーの宿主となっている。本研究では、異種ウイルス(ハイポウイルス)の蛋白質(p29)で誘発可能なレオウイルス(MyRV1)ゲノムセグメントの遺伝子再編成を多方向から解析した。今年度は上記目的のため以下の3項目を実施した(申請書に記載した実施項目3,4の実施状況については昨年度のC-7-1を参照されたい)。
    5p29のmiRNAへの影響
    糸状菌ではsiRNAは同定されているが、miRNAは未だ発見されていない。そこで、miRNAのクローニングを試みた。しかし、それらの同定には至らなかった。但し、これを相殺する成果として、アカパンカビあるいは植物でsiRNAの増幅に関与すると考えられるRNA依存RNA合成酵素遺伝子(rdr1)の破壊株を得るのに成功し、破壊株では再編成誘導が起らないことを明らかにすることに成功した。
    6植物ウイルス由来RNAサイレンシング抑制蛋自質の遣伝子属編成誘導能
    RNAサイレンシング抑制能と遺伝子再編成誘導能との関連が示された場合は、他のRNAサイレンシング抑制蛋白質(例えば、植物ウイルス由来のPotyvirus HC-Pro, cucumovirus 2b, tombusvirus p19)のMyRV1遺伝子再編成誘導能を検定した。しかし、結果は陰性であった。

    researchmap

  • 植物マイナス鎖RNAウイルスが誘導するバイロプラズムの形成機構と感染戦略上の役割

    研究課題/領域番号:21580056  2009年 - 2011年

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    近藤 秀樹, 鈴木 信弘, 丸山 和之, イダ バグスアンディカ

      詳細を見る

    配分額:4810000円 ( 直接経費:3700000円 、 間接経費:1110000円 )

    ランえそ斑紋ウイルス(OFV)はマイナス鎖RNAをゲノムに持ち,感染植物細胞に核内封入体バイロプラズムを誘導する.本研究では,このバイロプラズムの形成機構,特にその誘導に関わるウイルス側因子の同定を中心に検討した.アグロインフィルトレーション法を用いた一過的遺伝子発現実験を行ったところ,ヌクレオキャプシド蛋白質Nと推定リン酸化蛋白質Pを同時発現することで,ウイルス非感染下でもバイロプラズム様構造が誘導されることが示された.

    researchmap

  • 植物マイナス鎖RNAウイルスのゲノム輸送機構に関する研究

    研究課題/領域番号:19580048  2007年 - 2008年

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    近藤 秀樹, 鈴木 信弘

      詳細を見る

    配分額:4420000円 ( 直接経費:3400000円 、 間接経費:1020000円 )

    植物のマイナス鎖RNAウイルスであるOFVをモデルとし、ゲノム輸送に関わると考えられるウイルス因子を解析した。特にウイルスの移行形態とされるvRNP複合体を精製し、構成ウイルス蛋白質を明らかにした。そのうち、ゲノム結合タンパク質であるNの細胞核(複製の場と推定)への輸送にはアクセサリー蛋白質であるPが必要であることを発見した。また、モデル植物における細胞間移行や長距離移行に関する一連の研究も行った。これらの成果は、OFVのゲノム移行輸送機構を理解する上で基礎的な知見となると期待される。

    researchmap

  • 根におけるRNAサイレンシング調節機構の解明とウイルス抵抗性植物創成への応用

    研究課題/領域番号:07F07157  2007年 - 2008年

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 信弘, ANDIKA I.

      詳細を見る

    配分額:2200000円 ( 直接経費:2200000円 )

    本研究では,「根でのRNAサイレンシング活性に関与する宿主因子とそれを低減する因子」を解析した.まず,第一にトランスジーンやウイルスに対するサイレンシング活性が根で低いという現象の一般化を検討した.すなわち,数種のウイルスと宿主植物の組み合わせで調べ,根におけるウイルスのサイレンシング活性抑制効果についても詳細に解析した.次に,トランスジーン,ウイルス由来のsiRKAの根での蓄積量が低かったことから,その産生に関与するDCL群などの宿主因子を中心に,アラビドプシスの葉と根での発現パターンを解析した.さらに,候補となる宿主因子の変異体について,トランスジーンやウイルス等に対する影響を評価した.
    1.[根でのRNAサイレンシングにより影響される複製レベルはウィルスにより異なる]
    トランスジーンに対するサイレンシングが根で低いことは植物種を越えて一般化できるが,ウイルスに対するサイレング効果はウイルス種により異なっていた.
    2.[RNAサイレンシング関連遺伝子の変異体とウイルス感受性]
    PVXをアラビドプシス(非宿主とされていた)のdcl変異体へ接種したところ,DCL2とDCL4の変異体で効率的に全身移行したが,根ではDCL2,DCL4に加えDCL3もPVXの蓄積に影響することが示唆された.
    3.[サイレンシング関連遺伝子変異体における内在型siRNAの蓄積とトランスジーンサイレンシングへの影響]
    現在実施中.
    4.[サイレンシング活性低減要因(遺伝子)の根における強制的発現ラインの作出]
    活性の低減に関わる候補遺伝子BNYVV p14をN.benthamiana根で強制的に発現させた.

    researchmap

  • 宿主防御反応(PTGS)を打破するウイルス蛋白質のクリ胴枯病菌モデル系での解析

    研究課題/領域番号:14360030  2002年 - 2004年

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    鈴木 信弘

      詳細を見る

    配分額:14400000円 ( 直接経費:14400000円 )

    RNAサイレンシング(post-transcriptional gene silencing)は酵母からほ乳類まで広く存在する配列特異的RNA分解機構である。RNAサイレンシングの生物学的意義の一つが分子パラサイト(ウイルス、トランスポゾン、トランスジーン)に対する防御反応との見方が提起されている。一方、ウイルスもその賢知を示すかのように宿主防御と考えられるRNAサイレンシングに対抗するための抑制蛋白質をコードする事が植物ウイルス、動物ウイルスで証明されている。これまでのところ、抑制蛋白質は1本鎖RNAウイルス、1本鎖および2本鎖DNAウイルスで報告されているが、2本鎖RNAウイルスでの報告例はない。本課題は子のう菌であるクリ胴枯病菌PTGS発動株を用いて、クリ胴枯病菌感染性ハイポウイルスの他、植物、動物ウイルス由来のPTGS打破作用を有する蛋白質を同定し、性格付けを行なうことを目的としている。これまでに以下の成果を得た。
    1)抑制蛋白質候補遺伝子を発現しているクリ胴枯病菌に異種ウイルスであるマイコレオウイルス1(MYRV1)を感染させ、その増殖量が高まるかどうかでPTGSサプレッサー活性を持つかどうかを検定した。その結果、ハイポウイルス由来パパイン様プロテアーゼp29を発現しているクリ胴枯病菌では、異種ウイルスであるMYRV1の増殖量が高まったのに対し、ハイポウイルスの複製を高めるp40の発現体ではMYRV1の増殖量変化がなかった。この検定法を用いて、植物ウイルスであると同時に昆虫ウイルスでもある植物レオウイルス(イネ萎縮ウイルス,RDV ; wound tumor virus, WTV)のゲノムセグメント12本の分節2本鎖RNAセグメントにコードされた蛋白質の検定を行った。その結果、MYRV1の複製を高める蛋白質は検出されなかった。
    2)ハイグロマイシン耐性遺伝子(HygR)を標的とするPTGSクリ胴枯病菌株の作出を試みた。既に恒常発現用GPDプロモーター支配下に連結したHygRで形質転換した株を得た。PTGS引き金として働くdsRNAを菌細胞内で合成させるため、完全長逆反復配列Hyg(イントロンを含む)でハイグロマイシン耐性株を二重形質転換を行った。二重形質転換体がPTGSを示すかどうかをハイグロマイシンに対して感受性になるかどうかで調べた。数百系統の形質転換体の内、十数株が感受性を示した。DNA解析を行った結果、二重形質転換体が逆反復配列を保持していることが示された。従って、Hyg感受性株はRNAサイレンシングによって感受性を示すことが示唆された。現在、これらの系統を用いて上記のウイルス蛋白質のRNAサイレンシング抑制活性を解析中である。

    researchmap

  • イネ萎縮ウイルス複製酵素複合体の再構成

    研究課題/領域番号:07856005  1995年

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

      詳細を見る

    配分額:900000円 ( 直接経費:900000円 )

    イネ萎縮ウイルスはファイトレオウイルスんい属するウイルスで、12本の文節2本鎖RNAをゲノムに持つ。昨年全ゲノムの構造解析が完了し(植物レオウイルスとしては最初である)、各ゲノムが4塩基の末端保存配列、逆反復配列、単一のORFから構成されることが示された。末端保存配列、逆反復配列の末端配列ドメインはゲノムセグメントの粒子への詰め込みと複製・転写に関与することが示唆されているが、明らかとなっていない。本研究では末端保存配列と相互作用するであろうウイルス蛋白質、宿主蛋白質の同定、さらにそれら蛋白質と相互作用するシス配列の同定を最終目的に、RNA合成活性を持つRDVコア粒子の再構成を試みた。
    RDVのコア粒子を構成する蛋白質遺伝子全て(S1、S3、S5 S7)を昆虫(夜盗蛾)細胞、Spodoptera frugiperdaで発現させることに成功した。さらに疑似コア粒子と考えられる構造物の部分精製も行った。但し、現在までのところ、この構造物のRNA合成活性は確認されていない。今後、コア粒子様構造物の精製法、鋳型導入法等の検討を要する。
    研究過程でRDV複製に関与するであろう酵素について興味深い発見をした。以下に記す。これまで、P2、P5はアウタ-レイヤー蛋白質と考えられていたが、昨年度の奨励研究Aによりマイナ-コア蛋白質をコードすることが明らかとなった。本研究によりこのP5がGTPと共有結合することが示された。P5はmRNA転写の際のキャッピング酵素、すなわちグアニル酸転移酵素あるることが示唆された。また、P5はGTPのほかにATP,UTPとの結合活性を持っていた。この活性はこれまで報告されているグアニル酸転移酵素とは大きく異なる性質である。ATP,UTP,GTPの結合サイトは同じか?、ATP,UTP結合能の生物学的意義は何か?等、今後の大きな検討課題である。

    researchmap

  • イネ萎縮ウイルスのヨコバイ細胞感染性を支配するウイルス因子の同定

    研究課題/領域番号:06856008  1994年

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

      詳細を見る

    配分額:900000円 ( 直接経費:900000円 )

    1)他の植物レオウイルスで得られた過去の結果と予備実験の結果から、S2、S5がウイルス粒子表面に存在する蛋白質(P2、P5)をコードすること、さらにこれらがウイルス精製過程で脱落するすることが示唆された。まず、これをP2、P5に対する抗体を用いて証明した。
    RDV S2(一部)、S5(完全長)のcDNAをそれぞれ夜盗蛾培養細胞中で発現させた。P2のカルボキシル基末端半分(60 kDa)と89kDaのP5の大量発現が確認された。これらのRDV由来の蛋白質をウサギに免疫し、抗血清を得た。免疫電顕法を用いてこれらの抗体と粒子との反応性を調べた結果、P2血清は粒子と反応したが、P5血清は反応しなかった。次にウエスタン法を用いて精製粒子、精製コア(内殻)粒子との反応性を調べた。P2血清は精製粒子中の130 kD蛋白質と反応したが、コア粒子中には反応する蛋白質は検出されなかった。一方、P5血清は精製粒子中の89kDマイナ-蛋白質と反応した。また、この蛋白質はP5抗体によってコア粒子からも微量検出された。
    これらの結果から、P2は予想された通り粒子の外殻粒子に存在するがP5については外殻蛋白質ではなく内殻粒子を構成する微量成分であることが明らかになった。S5については従来S2と同様に外殻蛋白質をコードすると考えられてきた。しかし、興味深いことにコア蛋白質をコードすることが証明された。植物レオウイルスの中で遺伝子-産物の全ての対応が明らかとなったのはRDVが初めてであり、世界的に評価されうる大きな成果である。
    従って、P2とこれまでに外殻蛋白質と同定されているP8がヨコバイ培養細胞感染性を支配することが示唆された。
    2)ヨコバイ培養細胞株の樹立。
    木村の方法(1987年)によってツマグロヨコバイ卵細胞の株化を試みた。初代培養には成功したが、安定継代株はえられていない。
    今後、安定なヨコバイ培養細胞株を樹立し、P2・P8抗体を用いたRDVの感染中和試験を行う必要がある。また、RDVS2コードのP2を完全な状態で組み換えバキュロウイルス発現夜盗蛾細胞から精製し、その精製P2を用いて完全粒子の再構成系を確立する必要がある。

    researchmap

  • 虫媒伝染性欠損に関与するイネ萎縮ウイルスの同定

    研究課題/領域番号:05856006  1993年

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

      詳細を見る

    配分額:900000円 ( 直接経費:900000円 )

    (1)イネ萎縮ウイルス(RDV)はヨコバイで永続的に媒介され、しかも虫体内で増殖し高率に経卵伝染する。しかし、本ウイルスを数年イネ植物体だけで継代することによって(感染イネを株分けで栽培し続ける)、伝染性が失われるという興味深い現象が見いだされる。本研究ではまず、この伝染性が欠落したRDV感染イネを得た。
    (2)野性型とヨコバイ伝染性欠損型RDVでゲノムセグメントの電気泳動像に違いがあるかどうかまず調べた結果、両者に差は認められなかった。
    (3)RDVゲノムセグメントがコードする蛋白質をバキュロウイルスベクターを用いて発現させ、それらに対する抗体を作製した。
    (4)3)で作製した抗体を用いて野性型、伝染性欠損型感染イネ中のRDV蛋白質の検出を行なった。イネ個体間で検出量に差は認められたが、野性型と欠損型との間では明確な違いは認められなかった。
    これらの結果は、他のレオウイルスで報告されているような特定セグメントの欠失によるのではなく、他のメカニズム(例えば点突然変異)によってRDVのヨコバイ伝染性欠損が起こることを示唆する。

    researchmap

  • ライボザイムを利用したイネ委縮病の防除

    研究課題/領域番号:04856011  1992年

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

      詳細を見る

    配分額:900000円 ( 直接経費:900000円 )

    researchmap

  • イネのロイシンジッパ-型DNA結合タンパク質の遺伝子構造と機能

    研究課題/領域番号:03262215  1991年 - 1993年

    日本学術振興会  科学研究費助成事業 重点領域研究  重点領域研究

    草野 友延, 鈴木 信弘

      詳細を見る

    配分額:1900000円 ( 直接経費:1900000円 )

    我々は,種々の環境ストレスのうち低温(5℃)がイネ植物に及ぼす影響を遺伝子発現のレベルの違いで検出しようと研究を進めてきた。独自に開発したcDNAライブラリ-作成法及びサブトラクション法〔Nucleic Acids Res.18:1071(1990),Plaut Cell Physiol.32:in press(1991)〕により低温処理で特異的に誘導される3種のイネcDNAクロ-ンの分離に成功してきた。これら3種のクロ-ンは、低温処理との関連が示唆されているアブシジン酸の処理あるいは高浸透圧(0.5Mマニト-ル)処理によっては誘導はおこらず,また熱ショク処理によっても誘導されない。更には1度低温で誘導されたものを常温25℃に戻してやると転写レベルでの誘導が全くおこっていない事も確認している。従ってこれらのクロ-ンは低温処理により特異的に発現が誘導されると結論した。
    これらのクロ-ンのうちLip19と命名したものは1323塩基対からなり,1次構造解析及びセンス鎖の同定などより約19,000の分子量のタンパク質をコ-ドし,その遺伝子産物はタンパク質デ-タベ-ス検索の結果トウモロコシ由来のジッパ-モチ-フをもつDNA結合タンパク質であることが明らかになっている。
    ゲノミックサザン法によりlip19遺伝子は1コピ-であることが判明した。λgtloの部分ライブラリ-より,ゲノミッククロ-ンを4kbのEcoRI断片として単離した。物理地図及び1次構造の解析により,lip19は翻訳領域,非翻訳領域を問わず挿入配列をもたないことが明らかとなった。現在,E.ColiのTクポリメラ-ゼ/Tクプロモ-タ-発現系で作らせたLip19を用い,DNA認識配列の同定を行っている。

    researchmap

  • イネ委縮ウイルスmRNAのイネおよび昆虫培養細胞への導入

    研究課題/領域番号:02856013  1990年

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

      詳細を見る

    配分額:900000円 ( 直接経費:900000円 )

    researchmap

  • イネ萎縮ウイルスの遺伝的解析

    研究課題/領域番号:01760048  1989年

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    鈴木 信弘

      詳細を見る

    配分額:900000円 ( 直接経費:900000円 )

    researchmap

▼全件表示

 

担当授業科目

  • ウイルス分子生物学演習 (2023年度) 前期  - その他

  • ウイルス分子生物学演習 (2023年度) 後期  - その他

  • ウイルス分子生物学演習 (2023年度) 後期  - その他

  • ウイルス分子生物学演習 (2023年度) 後期  - その他

  • ウイルス分子生物学演習 (2023年度) 前期  - その他

  • ウイルス分子生物学演習 (2023年度) 前期  - その他

  • ウイルス分子生物学特別演習 (2023年度) 通年  - その他

  • 植物-ウイルス/細菌相互作用 (2023年度) 前期  - 木5~8

  • 植物-ウイルス/細菌相互作用学 (2023年度) 前期  - 木9~12

  • 特別研究 (2023年度) その他  - その他

  • 生物資源科学特別研究 (2023年度) 通年  - その他

  • 発展ウイルス分子生物学 (2023年度) 前期  - その他

  • 発展ウイルス分子生物学 (2023年度) 前期  - その他

  • ウイルス分子生物学演習 (2022年度) 前期  - その他

  • ウイルス分子生物学演習 (2022年度) 後期  - その他

  • ウイルス分子生物学演習 (2022年度) 後期  - その他

  • ウイルス分子生物学演習 (2022年度) 前期  - その他

  • 植物ウイルス学 (2022年度) 第1学期  - 木3,木4

  • 植物-ウイルス/細菌相互作用学 (2022年度) 前期  - 木9~12

  • 生物資源科学特別研究 (2022年度) 通年  - その他

  • 発展ウイルス分子生物学 (2022年度) 前期  - その他

  • ウイルス分子生物学演習 (2021年度) 前期  - その他

  • ウイルス分子生物学演習 (2021年度) 後期  - その他

  • ウイルス分子生物学演習 (2021年度) 後期  - その他

  • ウイルス分子生物学演習 (2021年度) 前期  - その他

  • 植物ウイルス学 (2021年度) 第1学期  - 木3,木4

  • 植物-ウイルス/細菌相互作用学 (2021年度) 前期  - 木9~12

  • 生物資源科学特別研究 (2021年度) 通年  - その他

  • 発展ウイルス分子生物学 (2021年度) 前期  - その他

  • ウイルス分子生物学演習 (2020年度) 前期  - その他

  • ウイルス分子生物学演習 (2020年度) 後期  - その他

  • ウイルス分子生物学演習 (2020年度) 後期  - その他

  • ウイルス分子生物学演習 (2020年度) 前期  - その他

  • 植物ウイルス学 (2020年度) 第1学期  - 木3,木4

  • 植物-ウイルス/細菌相互作用 (2020年度) 前期  - その他

  • 生物資源科学特別研究 (2020年度) 通年  - その他

  • 発展ウイルス分子生物学 (2020年度) 前期  - その他

▼全件表示

 

社会貢献活動

  • ミクロの世界へようこそ、ウイルス学入門

    役割:講師

    岡山大学資源植物科学研究所  Summer Science School SSS2021  2021年8月2日

     詳細を見る

    種別:その他

    researchmap

  • ウイルスと生きる:SARS-CoV-2.

    役割:講師

    岡山大学資源植物科学研究所  Summer Science School online 2020  2020年11月21日

     詳細を見る

    種別:その他

    researchmap

  • 今だから聞けるウイルス学講座「菌類ウイルスに魅せられて」

    役割:講師

    ウイルス学会  湯河原ウイルスキャンプ  2018年6月6日 - 2018年6月7日

     詳細を見る

    種別:講演会

    researchmap

  • 岡山大学先端研究講座 ネオウイルス学:ウイルスと生きる

    役割:講師

    岡山大学  岡山大学先端研究講座  2017年6月17日

     詳細を見る

    種別:講演会

    researchmap

  • 研究室紹介

    役割:講師

    高校生研究所訪問  2017年3月14日

     詳細を見る

    種別:その他

    researchmap

  • ウイルスの有効利用〜ウイルスで病気を制す:「ヴァイロコントール」〜

    役割:情報提供

    岡山大学知恵の見本市2016  2016年11月11日

     詳細を見る

  • これでもウイルス?

    役割:講師

    独立行政法人国立病院機構仙台医療センター  みちのくウイルス塾  2016年7月16日 - 2016年7月17日

     詳細を見る

    種別:講演会

    researchmap

  • 職場体験

    役割:企画

    岡山大学資源植物科学研究所  2013年10月16日 - 2013年10月19日

     詳細を見る

    種別:その他

    researchmap

  • 日本学術振興会(JSPS)サイエンス・ダイアログ プログラム

    役割:情報提供

    日本学術振興会(JSPS)  2013年2月14日

     詳細を見る

    種別:講演会

    researchmap

  • マイコウイルスとヴァイロコントロール

    役割:講師

    日本植物病理学会  平成24年度植物感染生理談話会  2012年8月30日

     詳細を見る

    種別:セミナー・ワークショップ

    researchmap

  • ウイルスいろいろ:目立たないウイルス、植物の守り神となるウイルス

    役割:講師

    独立行政法人国立病院機構仙台医療センター  みちのくウイルス塾  2012年7月14日 - 2012年7月15日

     詳細を見る

    種別:講演会

    researchmap

  • ウイルスいろいろ:役立つウイルス、目立たないウイルス

    役割:講師

    岡山大学資源植物科学研究所  平成24年度 公開講座「ウイルス -摩訶不思議な不完全生命体-」  2012年5月26日

     詳細を見る

    種別:講演会

    researchmap

  • ヴァイロコントロール:悪玉カビを病気にする善玉ウイルス

    役割:講師

    日本ウイルス学会  ウイルス学キャンプ  2010年8月9日 - 2010年8月10日

     詳細を見る

    種別:講演会

    researchmap

  • 病害虫防除の最前線」植物の病気の生物防除=ウイルスをもってカビを制す=

    役割:講師

    山陽放送  吉備創生カレッジ講座  2009年6月27日

     詳細を見る

    種別:講演会

    researchmap

  • 賢いウイルスとその遺伝子

    役割:講師

    岡山大学  岡山大学公開講座  2002年7月

     詳細を見る

    種別:講演会

    researchmap

▼全件表示