Faculty Profiles - FUJIWARA Toshiyoshi

MISC - FUJIWARA Toshiyoshi

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Down-regulation of nuclear protein ICBP90 by p53/p21Cip1/WAF1-dependent DNA-damage checkpoint signals contributes to cell cycle arrest at G1/S transition.

Arima, Y, Hirota, T, Bronner, C, Mousli, M, Fujiwara, T, Niwa, S, Ishikawa, H, Saya, H

Genes Cells 2004

Quantitative analusis of p53-targeted gene expression and visualization of p53 trascriptional activity following intratumoral administration of adenoviral p53 in vivo

Ohtani S, Kagawa S, Tango Y, Umeoka T, Tokunaga N, Tsunemitsu Y, Jack A. Roth, Taya Y, Tanaka N, Fujiwara T

Molecular Cancer Therapeutics 2004

Telomerase-Specific Repliccation-Selective Virotherapy for Human Cancer

Kawashima T, Kagawa S, Kobayashi N, Shirakiya Y, Umeoka T, Teraishi F, Taki M, Kyo S, Tanaka N, Fujiwara T

Clinical Cancer Research 2004

進行非小細胞肺癌に対してp53遺伝子治療を施行し奏効が得られた1例

香川俊輔, 藤原俊義, 大谷彰一郎, 田中紀章, 岡山大病院, 遺伝子, 細胞治療センタ

癌と化学療法 2004

化学療法 3 切除不能再発胃癌患者に対するIFP療法

松岡順治, 西崎正彦, 香川俊輔, 藤原俊義, 合地明, 猶本良夫, 田中紀章, 大, 消化器腫瘍外

日本癌治療学会誌 2004

遺伝子治療胃癌腹膜播種モデルにおけるBax遺伝子の抗腫瘍効果

香川俊輔, 藤原俊義, 常光洋輔, 徳永尚之, 大谷彰一郎, 梅岡達生, 西崎正彦, 田中紀章, 岡山大遺伝子, 細胞治療センタ

日本癌治療学会誌 2004

Late resistance to adenoviral p53-mediated apoptosis caused by decreased expression of Coxsackie-adenovirus receptors in human lung cancer cells

Tango Y, Taki M, Shirakiya Y, Ohtani S, Tokunaga N, Tsunemitsu Y, Kagawa S, Tani T, Tanaka N, Fujiwara T

Cancer Sci 2004

Visualization of Intrathoracically Disseminated Solid Tumors in Mice with Optical Imaging by Telomerase-Specific Amplification of a Transferred Green Fluorescent Protein Gene

Umeoka T, Kawashima T, Kagawa S, Teraishi F, Taki M, Nishizaki M, Kyo S, Nagai K, Urata Y, Tanaka N, Fujiwara T

Cancer Reaserch 2004

A novel method for gene delivery and expression in esophageal epithelium with fibrin glues containing replication-deficient adenovirus vector

F Teraishi, T Umeoka, T Saito, T Tsukagoshi, N Tanaka, T Fujiwara

SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES 17 ( 11 ) 1845 - 1848 2003.11

Language：English Publisher：SPRINGER-VERLAG

Background: Gene transfer to the esophageal epithelium holds the potential for the therapy of malignant as well as premalignant lesions in the upper gastrointestinal tract. Replication-deficient recombinant adenoviruses represent an efficient means of introducing genes in vivo into cells in a variety of organs. The majority of in vivo studies utilize direct submucosal injection for delivery of the viral vectors into the locoregional area of the gut; transferring genes into epithelial cells, however, is difficult because viruses are retained in the subepithelial space. To establish the efficient method for gene transfer into the epithelial cells, we have developed a multiluminal spray catheter that can be passed through the accessory channel of an endoscope, and we have evaluated the feasibility of fibrin glues as a vehicle of recombinant adenoviruses in a porcine model.
Methods: The fibrinogen solution and the thrombion solution containing an E1/E3 deleted recombinant adenovirus expressing the bacterial lacZ gene (Ad-lacZ) were endoscopically sprayed on the porcine esophagus through the catheter attached to the dual-barrel syringe. Twenty-four hours after gene delivery, beta-galactosidase activity of the esophagus was determined under the microscope following X-gal staining.
Results: The fibrin glue could be locally sprayed on the porcine esophagus by using the multichannel catheter through the endoscope. Attachment of the fibrin glue comtining Ad-lacZ caused strong beta-galactosidase staining on epithelial cells in the mucosal surface, but not in the basal cell layer.
Conclusion: Endoscopic local delivery of recombinant adenoviruses in aerosolized fibrin glues through a multiluminal catheter could provide an optimal technique for gene transfer into epithelial cells in the mucosal surfece, which may have important implications for the treatment of human esophageal premalignant diseases.

DOI： 10.1007/s00464-003-8146-5

Web of Science

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RGDファイバー変異を挿入した腫よう特異的増殖可能アデノウイルス(TRAD)の抗腫よう効果の増強

滝正樹, 藤原俊義, 川嶋健, 岸本浩行, 藤原俊哉, 水口裕之, 京哲, 香川俊輔, 西崎正彦

日本癌学会総会記事 62nd 161 2003.8

蛍光イメージングによる癌特異的GFP遺伝子発現の検出: 癌特異的増殖可能アデノウイルスを用いた非侵襲的な生体内癌組織診断

梅岡達生, 藤原俊義, 川嶋健, 常光洋輔, 京哲, 香川俊輔, 西崎正彦, 田中紀章

日本外科学会雑誌 104 183 2003.4

制限増殖型アデノウイルスによる術前・術後補助療法の可能性: テロメラーゼ発現ヒト癌細胞における選択的腫よう融解による抗腫よう効果の検討

川嶋健, 藤原俊義, 滝正樹, 梅岡達生, 京哲, 西崎正彦, 香川俊輔, 田中紀章

日本外科学会雑誌 104 530 - 531 2003.4

癌に対する遺伝子治療の現況と展望 : 非増殖型ベクターから制限増殖型ベクターへ,さらに局所療法から全身療法へ

藤原俊義, 香川俊輔, 西崎正彦, 田中紀章

日本外科学会雑誌 104 191 - 192 2003.4

胃癌細胞に対するBaxアデノウイルスベクターの抗腫瘍効果 : 胃癌腹膜播種に対する補助療法としての可能性

常光洋輔, 香川俊輔, 渡辺貴紀, 藤原俊義, 田中紀章

日本外科学会雑誌 104 252 - 252 2003.4

ヒト末梢血単核球由来の樹状細胞とp53遺伝子あるいはp53蛋白質を用いた新しい免疫療法の開発

徳永尚之, 藤原俊義, 村上敬祥, 西崎正彦, 香川俊輔, 田中紀章

日本外科学会雑誌 104 527 - 527 2003.4

p53遺伝子治療におけるp53標的遺伝子発現の定量的解析とp53転写活性の可視化の試み : 術前アジュバント療法を目指して

大谷彰一郎, 藤原俊義, 梅岡達生, 丹後泰久, 香川俊輔, 西崎正彦, 田中紀章

日本外科学会雑誌 104 258 - 258 2003.4

Ectopic p21(sdi1) gene transfer induces retinoic acid receptor beta expression and sensitizes human cancer cells to retinoid treatment

F Teraishi, Y Kadowaki, Y Tango, T Kawashima, T Umeoka, S Kagawa, N Tanaka, T Fujiwara

INTERNATIONAL JOURNAL OF CANCER 103 ( 6 ) 833 - 839 2003.3

Language：English Publisher：WILEY-LISS

The biological effects of retinoic acid (RA) are mediated by nuclear retinoic acid receptors (RARs) that function as ligand-activated transcriptional factors. The response of human cancer cells to RA is known to be associated with the expression of RARbeta. Recent studies have demonstrated that the loss of RARbeta expression is involved in the development of a variety of human malignancies. We show that recombinant adenovirus-mediated p21(sdi1) gene transfer enhances RARbeta mRNA expression as well as protein expression and induces the sensitivity to all-trans RA (ATRA) in human cancer cells. Semi-quantitative reverse transcription-polymerase chain reaction analysis demonstrated that infection with adenovirus carrying human p21(sdi1) gene (AdSCMV-p21), which encodes a cyclin-dependent kinase inhibitor, induced RARbeta mRNA and protein expression in H1299 human non-small cell lung cancer cells and DLD-1 human colorectal cancer cells. We also found that exogenous introduction of the p21(sdil) gene transcriptionally activated the upstream promoter function of the RARbeta gene. Treatment with I muM of ATRA showed no significant inhibitory effects on the growth of H 1299 and DLD-1 cells; after Ad5CMV-p21 infection, however, cells underwent apoptosis with ATRA treatment at the same concentration, suggesting that p21(sdil) gene transfer sensitized H1299 and DLD-1 cells, presumably, through RARbeta upregulation. We also demonstrated the efficacy of intratumoral injection of AdSCMV-p21 in combination with systemic administration of ATRA in a nude mice xenograft model. Our results indicate that recombinant adenovirus-mediated p21(sdil) gene transfer could be potentially useful for the local induction of RA sensitivity in human premalignant and malignant lesions lacking appropriate RARbeta expression. (C) 2002 Wiley-Liss, Inc.

DOI： 10.1002/ijc.10892

Web of Science

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Protein transduction domains enable isolated islets to efficiently internalize the target protein

T Okitsu, N Kobayashi, T Totsugawa, M Maruyama, H Noguchi, T Watanabe, T Matsumura, T Fujiwara, N Tanaka

TRANSPLANTATION PROCEEDINGS 35 ( 1 ) 479 - 479 2003.2

Successful lentivirus-based delivery of a LacZ gene into human endothelial cells

T Totsugawa, N Kobayashi, M Maruyama, T Okitsu, H Noguchi, T Watanabe, T Matsumura, T Fujiwara, N Tanaka

TRANSPLANTATION PROCEEDINGS 35 ( 1 ) 499 - 500 2003.2

Lentiviral vector mediated gene delivery into non-dividing isolated islet cells

T Okitsu, N Kobayashi, T Totsugawa, M Maruyama, H Noguchi, T Watanabe, T Matsumura, T Fujiwara, N Tanaka

TRANSPLANTATION PROCEEDINGS 35 ( 1 ) 483 - 483 2003.2

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