Authorship：Lead author   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

The mouse Harderian gland (HG) is a secretory gland that covers the posterior portion of the eyeball, opening at the base of the nictitating membrane. The HG serves to protect the eye surface from infection with its secretions. Mice open their eyelids at about 2 weeks of age, and the development of the HG primordium mechanically opens the eye by pushing the eyeball from its rear. Therefore, when HG formation is disturbed, the eye exhibits enophthalmos (the slit-eye phenotype), and a line of Fgf10+/− heterozygous loss-of-function mice exhibits slit-eye due to the HG atrophy. However, it has not been clarified how and when HGs degenerate and atrophy in Fgf10+/− mice. In this study, we observed the HGs in embryonic (E13.5 to E19), postnatal (P0.5 to P18) and 74-week-old Fgf10+/− mice. We found that more than half of the Fgf10+/− mice had markedly degenerated HGs, often unilaterally. The degenerated HG tissue had a melanized appearance and was replaced by connective tissue, which was observed by P10. The development of HGs was delayed or disrupted in the similar proportion of Fgf10+/− embryos, as revealed via histology and the loss of HG-marker expression. In situ hybridization showed Fgf10 expression was observed in the Harderian mesenchyme in wild-type as well as in the HG-lacking heterozygote at E19. These results show that the Fgf10 haploinsufficiency causes delayed or defective HG development, often unilaterally from the unexpectedly early neonatal period.

DOI： 10.3390/jdb12020016

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Language：English   Publishing type：Research paper (scientific journal)  

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Language：Japanese   Publishing type：Thesis (other)  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：KARGER  

Culture medium containing dipicolinic acid at final concentrations of 0, 1, 2 and 4 mM were inoculated with the Miyagino strain of Bacillus subtilis natto, the bacteria used for the production of natto, and subjected to stationary culture for 24 hours, after which the activity of nattokinase was investigated using two methods: a standard fibrin plate method, and the synthetic substrate Suc-Ala-Ala-Pro-Phe-pNA. Using the standard fibrin plate method, it was confirmed that nattokinase activity rose to 12.5 FU/ml in the samples containing 2 mM dipicolinic acid, a value 5.6 times greater than that of the sample containing no dipicolinic acid (2.25 FU/ml). Colorimetric measurements using the synthetic substrate showed that the activity level was highest (168.5 nmol/min/ml) with addition of 2 mM dipicolinic acid, about 4.3 times the activity of the sample with no dipicolinic acid (39.6 nmol/min/ml). The effects of dipicolinic acid were also clear for the Naruse strain of Bacillus subtilis natto. Based on the results of comparison tests using DPA and nine structurally related compounds, including nicotinic acid, it appears that dipicolinic acid is capable of enhancing specific nattokinase production to a considerable extent.

DOI： 10.3136/fstr.12.152

Web of Science

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Language：Japanese   Publishing type：Research paper (scientific journal)  

日東菌及び目黒菌体中にジピコリン酸は多量に含まれていることが分かった。ビタミンK生産において最も条件の良い3%glyserolを加えた場合ジピコリン酸含量はコントロールより低くなる。また、日東菌から抽出した物質に抗H.pyroli菌作用が認められた。

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Language：Japanese  

CiNii Article

CiNii Books

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Other Link： http://search.jamas.or.jp/link/ui/2014247472

Language：Japanese   Presentation type：Oral presentation (general)  

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Language：Japanese   Presentation type：Poster presentation  

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Language：English   Presentation type：Poster presentation  

Venue：Cambrige, Ingland  

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Language：English   Presentation type：Poster presentation  

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Language：English   Presentation type：Poster presentation  

Venue：Geneva  

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