研究者詳細 - 木村　幸敬

論文 - 木村　幸敬

分割表示 109 件中 21 - 40 件目／全件表示 >>

Chemical conversion and liquid-liquid extraction of 5-hydroxymethylfurfural from fructose by slug flow microreactor 査読

Toshinori Shimanouchi, Yoshitaka Kataoka, Tatsuya Tanifuji, Yukitaka Kimura, Satoko Fujioka, Koichi Terasaka

AIChE Journal 62 ( 6 ) 2135 - 2143 2016年6月

掲載種別：研究論文（学術雑誌）

A dehydration of fructose in the water/methyl isobuthyl ketone (MIBK) biphasic system can yield 5-hydroxymethylfurfural (HMF) to be successfully extracted into the organic MIBK phase. The HMF production and yield in MIBK phase was discussed by using a simplified model taking into consideration of the slug flow. The extraction resistance of HMF across the interface between water and MIBK depended on the line velocity and the flow rate ratio. It was likely that the velocity field generated in the slug flow contributed to an increase in the mass transfer of HMF.

DOI： 10.1002/aic.15201

Surface properties of woody thin boards composed of commercially available lignin and cellulose: Relationship between the orientation of lignin and water repellency 査読

Toshinori Shimanouchi, Tomoya Kamba, Wei Yang, Satoka Aoyagi, Yukitaka Kimura

Applied Surface Science 347 406 - 413 2015年8月

担当区分：責任著者掲載種別：研究論文（学術雑誌）

Woody thin boards were prepared from lignin, cellulose, and water by compression molding at 180°C and 25 MPa for 10 min. Boards with higher contact angles gave lower values of relative permittivity on their surface. Attenuated-total reflection Fourier transfer infrared spectroscopy suggested that more lignin existed on the surface of the boards with the high contact angle, which was also supported by scanning electron microscopy and atomic force microscopy. Our findings thus revealed that the orientation of lignin at the surface resulted in increased hydrophobicity of the surface and contributed to the enhancement of water repellency.

DOI： 10.1016/j.apsusc.2015.03.139

Elevating the fuel properties of Humulus lupulus, Plumeria alba and Calophyllum inophyllum L. through wet torrefaction 査読

Wei Yang, Toshinori Shimanouchi, Miki Iwamura, Yuki Takahashi, Ryota Mano, Kohei Takashima, Tatsuya Tanifuji, Yukitaka Kimura

Fuel 146 88 - 94 2015年4月

担当区分：責任著者掲載種別：研究論文（学術雑誌）

Wet torrefaction is an effective process to improve the fuel properties of a biomass. However, different biomasses have different component weight ratios and it is possible that not all biomasses are suitable for wet torrefaction treatment. Here we conducted wet torrefaction using three types of biomass with different component weight ratios: Humulus lupulus (HL, the common hop), Plumeria alba (PA, an evergreen shrub) and Calophyllum inophyllum L. (CIL, an evergreen tree). The fuel properties of the obtained solid fuels were characterized. We found that lignin made the main contribution to the solid fuel yield. The reactivity of cellulose and hemicellulose in each biomass was affected by the biomass species and the component weight ratio of the biomass. The wet torrefaction was observed to efficiently elevate the fuel properties of carbon content, atomic H/C and O/C ratios, higher heating value (HHV) and hydrophobicity of all three types of biomass. The HHVs of the solid fuels prepared at 260 °C are comparable to those of commercial coals such as Northumerland No. 81/2 Sem. Anth. Coal, Jhanjra Bonbahal Seam Coal-R-VII, and German Braunkohole lignite. These solid fuels could be co-combusted with German Braunkohole lignite without a significant change in the combustion properties of German Braunkohole lignite because of their similar atomic H/C and O/C ratios as well as HHVs. At the wet torrefaction temperature of 260 °C, the solid fuel delivered from CIL had lower HHV compared to those from HL and PA although CIL contained the highest lignin content, which has a higher HHV than those of cellulose and hemicellulose. Scanning electron microscopy images of the solid fuels revealed that wet torrefaction was able to completely destroy the biomass surface and create numerous pores and cracks on the solid fuels surface, indicating that the solid fuels have the potential to be used as a source of carbon materials such as activated carbon in addition to their use as bio-fuels.

DOI： 10.1016/j.fuel.2015.01.005

Characterization of the residue and liquid products produced from husks of nuts from carya cathayensis sarg by hydrothermal carbonization 査読

Wei Yang, Toshinori Shimanouchi, Yukitaka Kimura

ACS Sustainable Chemistry and Engineering 3 ( 4 ) 591 - 598 2015年4月

担当区分：責任著者掲載種別：研究論文（学術雑誌）

Hydrothermal carbonization is considered to be an environment-friendly method to pretreat biomass. Therefore, hydrothermal carbonization of the husks of nuts from Carya cathayensis Sarg, at various temperatures and pHs were performed and the fuel properties of the obtained residue, water-soluble products (WSP) and acetone-soluble products (ASP) were characterized. It was found that the residue yield decreased with increasing hydrothermal carbonization temperature while the yield of total soluble products (WSP and ASP) was nearly the same. The residue and total soluble products yields had no large changes in the pH range of 4.0-10.0, but fewer residues and more total soluble products were obtained at pH 13.0. It was confirmed that hemicellulose was more reactive than cellulose and lignin during hydrothermal carbonization and that lignin could be effectively hydrolyzed/decomposed at pH 13.0. The higher heating values (HHVs) of residues were in the ranges of 22.0 to 28.2 MJ kg-1 at temperatures from 180 to 260 °C and pH from 4.0 to 10.0. The HHVs of these residues could be comparable with those of some commercial coals, such as lignite. However, the HHVs of residues decreased when the pH reached to 13.0. The WSP and ASP showed lower HHVs compared with heavy fuel oils, which meant the HHVs of WSP and ASP should be increased before being applied as bio-fuels.

DOI： 10.1021/acssuschemeng.5b00103

Influence of sugar surfactant structure on the encapsulation of oil droplets in an amorphous sugar matrix during freeze-drying 査読

S. Nakayama, Y Kimura, S. Miki, J. Oshitani, T. Kobayashi, S. Adachi, T. Matsuura, H. Imanaka, N. Ishida, H. Tada, K. Nakanishi, K. Imamura

Food Research International 70 143 - 149 2015年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

The encapsulation of O/W emulsion droplets in a freeze-dried amorphous sugar matrix was investigated, focusing on the impact of the molecular structure of the emulsifying surfactant. O/W emulsions, containing various surfactants, were freeze-dried in the presence of a sugar. Thirty types of surfactants, including eighteen different sugar surfactants and ten types of commercially available sugar ester mixtures, were used. Linoleic acid methyl ester and trehalose were used as the oil phase and sugar. The amounts of oil droplets encapsulated in freeze-dried amorphous sugar matrix were analyzed by Fourier transform Infrared spectroscopy. Sugar surfactants were generally superior to the other classes of surfactants for oil droplet encapsulation during freeze-drying, and there was the optimum alkyl chain length of the sugar surfactant. Sugar esters generally exhibited greater oil encapsulation than sugar ethers. Larger sugar head group appeared to result in better encapsulation in the case of sugar esters, but the opposite tendency was found for sugar ethers. A limited combination of sugar surfactants (15% sucrose mono- and 85% di-stearate) resulted in the maximum oil droplet encapsulation efficiency although these surfactants are individually quite poor in the encapsulation and other tested combinations did not improve the encapsulation efficiency relative to their individual effectiveness. (C) 2015 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.foodres.2015.02.003

Characterization of hydrochar prepared from hydrothermal carbonization of peels of Carya cathayensis sarg 査読

Wei Yang, Toshinori Shimanouchi, Yukitaka Kimura

Desalination and Water Treatment 53 ( 10 ) 2831 - 2838 2015年3月

担当区分：責任著者掲載種別：研究論文（学術雑誌）

Hydrothermal carbonization of peels of Carya cathayensis sarg (PCCS) by subcritical water or acetone- and ethanol-modified subcritical water was carried out at the temperature from 280 to 360°C and coal-like hydrochar was obtained. The hydrochar yield decreased with increasing of treatment temperature, but the higher heating value (HHV) and carbon content of hydrochar were promoted at higher treatment temperature. The HHV of hydrochar was in range of 30–46 MJ/kg with an increase from 52.4 to 127.6%, compared to the original PCCS. The HHVs of hydrochars obtained at 360°C could be comparable with those of heavy fuel oil (42.9 MJ/kg) and diesel oil (45.7 MJ/kg). The O/C and H/C values of hydrochar were similar to those of lignite and subbituminous, except the ash content. Especially, the O/C and H/C values of hydrochars prepared at 360°C could be compatible with those of bituminous. When treated with acetone- or ethanol-modified subcritical water, a synergistic effect of acetone–water or ethanol–water for hydrothermal carbonization was observed; the liquefaction rate with acetone–water or ethanol–water mixture was larger than that of water or acetone or ethanol only. There was no significant change on the O/C and H/C values and HHV on the hydrochar, although more PCCS, was decomposed when using acetone–water or ethanol–water mixture.

DOI： 10.1080/19443994.2014.931537

Preparation of biodegradable foam from walnut shells treated by subcritical water 査読

Wei Yang, Ichiro Shimizu, Tsutomu Ono, Yukitaka Kimura

Journal of Chemical Technology and Biotechnology 90 ( 1 ) 44 - 49 2015年1月

担当区分：責任著者掲載種別：研究論文（学術雑誌）

BACKGROUND: Subcritical water treatment is considered an environment-friendly method to liquefy biomass to prepare valuable chemicals. Several researchers have studied the solid obtained after autohydrolysis treatment for use as reinforcement in biocomposities and was the base pulp and paper technologies. The novelty of this present research is the use of solid in the manufacture of starch foams. RESULTS: Subcritical water was used to extract hemicellulosic carbohydrate from walnut shells at temperatures from 160 to 260°C for 15min and at 200°C for various treatment times. The maximum yield was obtained at 200°C for 15min. The residue of the walnut shells obtained at the maximum carbohydrates extraction yield was used to prepare a biodegradable foam by compounding with corn starch using a technique similar to compression molding. It was found that replacing 20% of the corn starch by the residue had no deleterious effects on the density and morphology, as indicated by scanning electron microscopy. The prepared foams had a dense outer skin and a less dense interior with large, mostly open cells. The flexural strains were similar to that of foamed polystyrene and the flexural modulus and flexural stresses were larger. CONCLUSION: It was considered that the prepared biodegradable foam was a promising material to take the place of plastic products in the future.

DOI： 10.1002/jctb.4451

Investigation of the degradation kinetic parameters and structure changes of microcrystalline cellulose in subcritical water 査読

Wei Yang, Toshinori Shimanouchi, Shengji Wu, Yukitaka Kimura

Energy and Fuels 28 ( 11 ) 6974 - 6980 2014年11月

担当区分：責任著者掲載種別：研究論文（学術雑誌）

Cellulose is the most abundant nature polymer and the main component in all biomass. In this study, the degradation kinetic parameters and structure changes of microcrystalline cellulose (MCC), which served as fundamental studies for degradation of biomass in subcritical water, were investigated at the temperature range from 100 to 300 °C. The yield of the MCC residue began to decrease at 205 °C and reached the lowest value at 275 °C. However, it showed an increase at the temperature higher than 275 °C. The degradation area of MCC (205-275 °C) was separated into zones 1 and 2 with 245 °C as a boundary. The activation energy (E), pre-exponential factor (A), and reaction order (n) of MCC in each zone were 226.5 kJ mol-1, 2.3 × 1023 s-1, and 0.6 (zone 1) and 423.1 kJ mol-1, 9.0 × 1040 s-1, and 0.5 (zone 2), respectively. There showed a breaking point of 245 °C for the Arrhenius plot in the reaction area. The surface morphology of the MCC residue had no significant change below 260 °C, as indicated by scanning electron microscopy (SEM) images. However, it was completely destroyed at the temperature above 275 °C, and MCC residues with strong pore structures were obtained at higher temperatures. The structures of the crystalline region of the MCC residue below 275 °C had no significant change, although 89.2% MCC was degraded at 275 °C. However, they nearly disappeared at higher temperatures with the steeply reducing crystallinity index of MCC at the same time. As for the degradation mechanism of MCC, it was proposed that only the surface of MCC was degraded at 205-245 °C and the hydrogen and glycosidic bonds on the interior of MCC fibrils were destroyed at the temperature range from 245 to 275 °C. Finally, the remaining MCC as well as generated oligomer and monomer were further degraded at higher temperatures.

DOI： 10.1021/ef501702q

Extraction of reducing sugar with anti-oxidative scavengers from peels of Carya cathayensis sarg.: Use of subcritical water 査読

Toshinori Shimanouchi, Shohei Ueno, Wei Yang, Yukitaka Kimura

Environmental Engineering Research 19 ( 1 ) 41 - 45 2014年3月

担当区分：責任著者掲載種別：研究論文（学術雑誌）

The peels of Carya cathayensis Sarg. (PCCS) were treated under subcritical water conditions (130°C to 280 °C for 0 to 120 min). The extract from PCCS included reducing sugar, proteins, and compounds with radical scavenging activity. Addressing the reducing sugar that is a resource of bioethanol, we could maximize the reducing sugar under the subcritical water (190°C for 60 min) and obtain 0.24 g/g-sample together with 9.7 units/mg-sample of radical scavenging activity. The obtained extract was estimated to correspond to 1 L of bioethanol/100 g-sample. It was therefore considered that the treatment by subcritical water could yield reducing sugar and natural compounds with radical scavenging activity. © 2014 Korean Society of Environmental Engineers.

DOI： 10.4491/eer.2014.19.1.041

Monodisperse polylactide microcapsules with a single aqueous core prepared via spontaneous emulsification and solvent diffusion 査読

Takaichi Watanabe, Yukitaka Kimura, Tsutomu Ono

RSC Advances 4 ( 10 ) 4872 - 4877 2014年

掲載種別：研究論文（学術雑誌）

A simple approach to preparing monodisperse poly(d,l-lactide) (PDLLA) microcapsules with a single aqueous core is developed. The method is based on automatic water-in-oil-in-water double emulsion formation from oil-in-water single emulsion via spontaneous emulsification which voluntarily disperses part of continuous aqueous phase into the dispersed oil phase dissolving oil-soluble amphiphilic diblock copolymer, poly(d,l-lactide)-b-poly(2-dimethylaminoethyl methacylate)(PDLLA-b-PDMAEMA), followed by coalescence of tiny water droplets within the polymer droplets, coupled with quick precipitation of polymers by diluting the emulsion with water. In this study, we have investigated the effect of PDLLA to PDLLA-b-PDMAEMA ratios and flow rates of each solution during preparing the emulsion on the final morphology and the size of the microcapsules. It was found that the polymer blend ratio played a crucial role in determining internal structure of the microcapsules. The microcapsules size decreased with the increment of the flow rate ratios of the continuous phase to the dispersed phase and eventually reached 10 μm, while maintaining narrow size distribution. In addition, we have demonstrated that the microcapsules can encapsulate both hydrophilic and hydrophobic compounds during the formation. © 2014 The Royal Society of Chemistry.

DOI： 10.1039/c3ra44066d

Water / Methyl isobutyl ketone (MIBK) biphasic system in slug flow under high temperature and pressure conditions 査読

Toshinori Shimanouchi, Tatsuya Tanifuji, Satoko Fujioka, Koichi Terasaka, Yukitaka Kimura

Solvent Extraction Research and Development 21 ( 2 ) 201 - 209 2014年

担当区分：責任著者掲載種別：研究論文（学術雑誌）

The clarification of the partition property of substrates in a water / organic biphasic system is useful in the development of a reaction / separation process for value-added materials, especially under high temperature and pressure conditions. In this study, the water / methyl isobuthyl ketone (MIBK) biphasic system was investigated under various temperatures (25 - 190 °C) at 10 MPa. The partitioning behavior of substrates such as furfural derivatives, amino acids, and saccharides, depended on the temperature and the flow rate ratio of MIBK to the water phase. The scale of hydrophobicity (HF) of the water / MIBK biphasic system to determine the partitioning behavior of substrates was estimated based on the partitioning behavior of amino acids. The HF value for the water / MIBK phase flow system of was greater than that for the batch system and comparable to that for conventional aqueous two-phase systems such as polyethylene glycol / dextran and liposome membrane systems. It was probably because an increase of the surface-to-volume ratio and the vortex field in the slug flow contributed to the mass transfer of substrates and their distribution.

DOI： 10.15261/serdj.21.201

Subcritical water-Assisted emulsification of oil/water: The effect of the solubility of the organic solvent and surfactants 査読

Toshinori Shimanouchi, Takashi Tange, Yukitaka Kimura

Solvent Extraction Research and Development 21 ( 2 ) 223 - 230 2014年

担当区分：責任著者掲載種別：研究論文（学術雑誌）

The formation of finely monodispersed oil-in-water (O/W) emulsions under high temperature and pressure conditions (240 °C and 10 MPa) depended on the solubility of the oil in water. Fatty acids with large solubilities in water favored the formation of oil droplets in emulsions of less than 100 nm in diameter. The water dissolved in the oil droplets participated throughout the cooling process, followed by the break-down of the oil droplets. In contrast, organic solvents with lower solubilities in water formed oil droplets in emulsions that had at most a diameter of 300 nm.

DOI： 10.15261/serdj.21.223

Subcritical water-assisted emulsification of decane/water: Influence of surfactants 査読

Toshinori Shimanouchi, Takashi Tange, Yukitaka Kimura

Solvent Extraction Research and Development 21 ( 1 ) 103 - 110 2014年

担当区分：責任著者掲載種別：研究論文（学術雑誌）

A finely monodispersed oil-in-water (O/W)emulsion was obtainedunder high temperature and pressureconditions(240 °C and 10 MPa). A coarse emulsion stabilized by Tween 20 was prepared in advance from the decane/water system to be treated under the subcritical conditions. The treated coarse emulsion was thereafter mixed with the aqueous solution including surfactants to improve the monodispersity of the oil droplets. The subcritical condition could supply sufficient energy to produce finely dispersed oil droplets (~233 nm in diameter).

DOI： 10.15261/serdj.21.103

Rapid conversion of glycerol to lactic acid under alkaline hydrothermal conditions, by using a continuous flow reaction system 査読

Toshinori Shimanouchi, Shouhei Ueno, Kazuki Shidahara, Yukitaka Kimura

Chemistry Letters 43 ( 4 ) 535 - 537 2014年

担当区分：責任著者掲載種別：研究論文（学術雑誌）

A rapid conversion of glycerol to lactic acid (lactate) could be successfully achieved under alkaline hydrothermal conditions, by using a continuous flow reaction system. A rapid conversion by a continuous flow-type reaction system made it possible to achieve the reaction under high-temperature conditions without side reactions. The rapid conversion and high reaction yield (reaction: 2min; yield: 90%) resulted from the rapid temperature-shift advantageous for the inhibition of side reactions. © 2014 The Chemical Society of Japan.

DOI： 10.1246/cl.131160

Microfluidic fabrication of monodisperse polylactide microcapsules with tunable structures through rapid precipitation 査読

Takaichi Watanabe, Yukitaka Kimura, Tsutomu Ono

Langmuir 29 ( 46 ) 14082 - 14088 2013年11月

掲載種別：研究論文（学術雑誌）

We describe a versatile and facile route to the continuous production of monodisperse polylactide (PLA) microcapsules with controllable structures. With the combination of microfluidic emulsification, solvent diffusion, and internal phase separation, uniform PLA microcapsules with a perfluorooctyl bromide (PFOB) core were successfully obtained by simply diluting monodisperse ethyl acetate (EA)-in-water emulsion with pure water. Rapid extraction of EA from the droplets into the aqueous phase enabled the solidification of the polymer droplets in a nonequilibrium state during internal phase separation between a concentrated PLA/EA phase and a PFOB phase. Higher-molecular-weight PLA generated structural complexity of the microcapsules, yielding core-shell microcapsules with covered with small PFOB droplets. Removal of the PFOB via freeze drying gave hollow microcapsules with dimpled surfaces. The core-shell ratios and the diameter of these microcapsules could be finely tuned by just adjusting the concentration of PFOB and flow rates on emulsification, respectively. These biocompatible microcapsules with controllable size and structures are potentially applicable in biomedical fields such as drug delivery carriers of many functional molecules. © 2013 American Chemical Society.

DOI： 10.1021/la403883a

Simplified model for extraction of 5-hydroxymethylfurfural from fructose: Use of water/oil biphasic system under high temperature and pressure conditions 査読

Toshinori Shimanouchi, Yoshitaka Kataoka, Masahiro Yasukawa, Tsutomu Ono, Yukitaka Kimura

Solvent Extraction Research and Development 20 205 - 212 2013年

担当区分：責任著者掲載種別：研究論文（学術雑誌）

5-Hydroxymethylfurfural (HMF) was obtained from the dehydration of fructose under high temperature and pressure conditions (180 °C and 10 MPa). To avoid the decomposition of HMF in the bulk aqueous phase, we dehydrated fructose in the water / metyl isobuthyl ketone (MIBK) biphasic system. The HMF could be successfully extracted into the organic MIBK phase without further decomposition of HMF. We present a simplified model taking into consideration of the slug flow with the wall film to maximize the HMF yield in the MIBK phase. It was likely that the slug flow contributed to an increase in the surface-to-volume ratio for the mass transfer of HMF.

DOI： 10.15261/serdj.20.205

Absorption of the indigestible disaccharide, β-1,4-mannobiose, from coconut by the Rat Portal Vein 査読

Hiroyuki Kanatani, Yukitaka Kimura, Masashi Asanoma, Akihiro Nakamura, Motohiko Hirotsuka, Shuji Adachi

Bioscience, Biotechnology and Biochemistry 76 ( 3 ) 575 - 577 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）

The intestinal absorption of β-1,4-mannobiose by rats was investigated. Mannobiose was detected in the portal vein plasma by matrix-assisted laser desorption ionization/time of flight mass spectrometry after its administration to rats. The presence of mannobiose in the rat plasma was confirmed by an experiment using β-mannosidase. These results indicate that mannobiose was directly absorbed through the intestines even without being hydrolyzed.

DOI： 10.1271/bbb.110731

Continuous fabrication of monodisperse polylactide microspheres by droplet-to-particle technology using microfluidic emulsification and emulsion-solvent diffusion 査読

Takaichi Watanabe, Tsutomu Ono, Yukitaka Kimura

Soft Matter 7 ( 21 ) 9894 - 9897 2011年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Monodisperse polylactide (PLA) microspheres were continuously fabricated by microfluidic emulsification and subsequent dilution in water. The diameter was precisely tuned from 6 to 50 μm by changing the flow rate of the fluids in microfluidics or the PLA concentration in the dispersed phase. The use of amphiphilic oil-soluble poly(ethylene glycol)-b-polylactide (o-PEG-PLA) as a matrix resulted in a highly porous microsphere morphology, and the porosity was controlled by blending PLA. Therefore, monodisperse PLA microspheres with the predetermined surface porosity were continuously produced by just enough reagents and energy. © 2011 The Royal Society of Chemistry.

DOI： 10.1039/c1sm05910f

Decomposition and discoloration kinetics of L-ascorbic acid powders in superheated steam 査読

Yuka Horagai, Lan-hsin Hung, Yukitaka Kimura, Shuji Adachi

LWT-FOOD SCIENCE AND TECHNOLOGY 41 ( 10 ) 2113 - 2117 2008年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

The decomposition and discoloration of powdery L-ascorbic acid in superheated steam were observed in the temperature range of 140-180 degrees C. For comparison, the decomposition and discoloration were also measured in the temperature range in hot air and at 180 degrees C in nitrogen. L-Ascorbic acid decomposed faster in superheated steam than in hot air. Discoloration was also more remarkable for the L-ascorbic acid treated in the superheated steam. The Weibull equation was applicable to the decomposition in both superheated steam and hot air. The early stage of discoloration was assumed to be expressed by zeroth-order kinetics. In the latter stage, fading occurred and the process was expressed by first-order kinetics which was modified to level off at a certain value. (C) 2007 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.lwt.2007.11.014

Synthesis of glyceryl ferulate by immobilized ferulic acid esterase 査読

Takemasa Matsuo, Takashi Kobayashi, Yukitaka Kimura, Moriyasu Tsuchiyama, Tadanobu Oh, Tatsuji Sakamoto, Shuji Adachi

BIOTECHNOLOGY LETTERS 30 ( 12 ) 2151 - 2156 2008年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPRINGER

Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL "Amano" from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50 degrees C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25 degrees C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.

DOI： 10.1007/s10529-008-9814-2

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