Organization
Faculty of Environmental, Life, Natural Science and Technology Professor
Position
Professor
Homepage
External link
IMAMURA Koreyoshi
Degree
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Doctor of Engineering ( 1999.6 Kyoto University )
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Master of Engineering ( Kyoto University )
Research Interests
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material physical chemistry
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Biochemical engineering
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Drying technology
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Food Technology
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Chemical Engineering
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食品工学
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化学工学
Research Areas
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Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering / protein, hydrophobic drug, enzyme reaction
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Life Science / Pharmaceutical analytical chemistry and physicochemistry / amorphous solid dispersion, glass transition
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Life Science / Food sciences / food engineering
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Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Transport phenomena and unit operations / drying, amorphous sugar, solid dispersion
Education
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Kyoto University
- 1997
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Kyoto University 工学研究科 化学工学
- 1997
Country： Japan
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Kyoto University
- 1992
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Kyoto University 工学部 化学工学科
- 1992
Country： Japan
Research History
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Okayama University Faculty of Environmental, Life, Natural Science and Technology Professor PhD
2023.4
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Okayama University Faculty of Natural Science and Technology Professor PhD
2021.4 - 2023.3
Country：Japan
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Okayama University Graduate School of Natural Science and Technology Professor
2012.6 - 2021.3
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Okayama University Graduate School of Natural Science and Technology Associate Professor
2005.4 - 2012.5
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Okayama University Faculty of Engineering associate professor
2003.12 - 2005.3
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- Cleaning of solid surfaces using radical
1998
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Okayama University Faculty of Engineering assistant professor
1997.4 - 2003.11
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- Adsorption of protein, peptide, &
1997
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- Stabilization of protein by sugar
1995
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oxidizing reaction
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amino acidly auto solid surfaces
Professional Memberships
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日本生物工学会
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日本農芸化学会
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日本食品工学会
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化学工学会
Committee Memberships
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化学工学会男女共同参画委員会 副委員長
2023.4
Committee type：Academic society
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Japan Society for Food Engineering director
2017.7
Committee type：Academic society
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日本生物工学会 西日本支部会計
1999
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日本食品工学会 理事
1906.1
Papers
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Evaluating the effects of electrolytes on the interaction forces between alumina surfaces in polyacrylic acid solutions using atomic force microscopy Reviewed
Naoto Kishimoto, Ryota Kaji, Katsumi Tsuchiya, Koreyoshi Imamura, Naoyuki Ishida
ADVANCED POWDER TECHNOLOGY 36 ( 10 ) 2025.10
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Chika Araga, Kaito Fukushima, Haruna Sato, Nao Honda, Takato Hasegawa, Koichi Nakaso, Naoyuki Ishida, Koreyoshi Imamura
Drying Technology 43 ( 6 ) 1108 - 1116 2025.4
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Pre-folding purification procedures for inclusion body-derived non-tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding. Reviewed International journal
Shuichiro Kimura, Wataru Yamamoto, Ai Miyamoto, Koreyoshi Imamura, Junichiro Futami
Biotechnology progress e3532 2025.1
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Avoidance of crystallization of hydrophobic drugs in an amorphous solid dispersion during spray-drying and storage Reviewed
Yamamoto, Rina, Imanaka, H, Ishida, Naoyuki, Imamura, Koreyoshi
Drying Technology 42 ( 13 ) 2022 - 2031 2024.11
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Nitta, Yuna, Sato, H, Yamamoto, Rina, Imanaka, Hiroyuki, Ishida, Naoyuki, Imamura, K
Drying Technology 42 ( 2 ) 227 - 237 2024.1
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Water sorption and glass-to-rubber transition of amorphous sugar matrices, vacuum foam- and spray-dried from alcohols Reviewed
Koji Takeda, Shinta Miyazaki, Takashi Okamoto, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
JOURNAL OF FOOD ENGINEERING 349 2023.7
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Evaluation of interaction forces between hydrophobic surfaces in surfactant solutions by atomic force microscopy Reviewed
Shota Kage, Koreyoshi Imamura, Naoyuki Ishida
Colloid and Polymer Science 301 ( 7 ) 775 - 782 2023.7
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Evaluation of Hydrophobic Attraction between Polystyrene Layer and Silanated Silica Surface by Atomic Force Microscopy Reviewed
Yuta Okuda, Koreyoshi Imamura, Naoyuki Ishida
Chemistry Letters 52 ( 12 ) 878 - 882 2023
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Kinetics of Enzymatic Reactions at the Solid/Liquid Interface in Nanofluidic Channels Reviewed
Koki Yamamoto, Kyojiro Morikawa, Hiroyuki Imanaka, Koreyoshi Imamura, Takehiko Kitamori
Analytical Chemistry 2022.10
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Extraordinary high preservation of the dispersion state of Au nanoparticles during freeze-thawing and freeze-drying with gum arabic Reviewed
Miki Kadowaki, Tsutashi Matsuura, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Colloids and Surfaces A: Physicochemical and Engineering Aspects 639 2022.4
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Comparison of improvements of aqueous dissolution of structurally analogous hydrophobic drugs by amorphous solid dispersion Invited Reviewed
Takashi Okamoto, Kayoko Yamamoto, Takanari Sekitoh, Akiho Fujioka, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Colloids and Surfaces A: Physicochemical and Engineering Aspects 632 127744 - 127744 2022.1
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Akiho Fujioka, Rina Yamamoto, Olivier Tramis, Hiroaki Ishida, Tsutomu Ono, Naoyuki Ishida, Koreyoshi Imamura
Drying Technology 1 - 13 2021.12
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Foaming characteristics of sugar- and polyvinylpyrrolidone-alcohol solutions during vacuum foam drying: A rheological approach Reviewed
Olivier Tramis, Akiho Fujioka, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Colloids and Surfaces A: Physicochemical and Engineering Aspects 627 127174 - 127174 2021.10
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Inhibiting Au nanoparticle aggregation in freeze-thawing by presence of various additives Reviewed
Miki Kadowaki, Hidetaka Yokota, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Advanced Powder Technology 32 ( 10 ) 3517 - 3524 2021.10
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Direct measurements of interaction forces of bovine serum albumin and lysozyme with stainless steel by atomic force microscopy Reviewed
Hikaru Kawaguchi, Hiroyuki Imanaka, Koreyoshi Imamura, Naoyuki Ishida
Colloids and Surfaces A: Physicochemical and Engineering Aspects 627 127137 - 127137 2021.10
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Takanari Sekitoh, Takashi Okamoto, Akiho Fujioka, Olivier Tramis, Koji Takeda, Tsutashi Matsuura, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Drying Technology 39 ( 14 ) 2065 - 2074 2021.9
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Crystallization characteristics of amorphous trehalose dried from alcohol Reviewed
Takanari Sekitoh, Takashi Okamoto, Akiho Fujioka, Tomohiko Yoshioka, Shinji Terui, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Journal of Food Engineering 292 110325 - 110325 2021.3
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Direct measurement of interaction force between hydrophilic silica surfaces in triblock copolymer solutions with salt by atomic force microscopy Reviewed
Takumi Deguchi, Takato Nakahara, Koreyoshi Imamura, Naoyuki Ishida
Advanced Powder Technology 32 ( 1 ) 30 - 36 2021.1
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Olivier Tramis, Akiho Fujioka, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Drying Technology 1 - 11 2020.9
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Study on the Stabilization Technique by Encapsulation of Biomolecules Such Food Proteins Invited Reviewed
Koreyoshi IMAMURA
Japan Journal of Food Engineering 21 ( 3 ) 95 - 111 2020.9
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A suitable and effective stepwise oxidative refolding procedure for highly‐cationic tetrameric avidin in nucleic acid free conditions Reviewed International journal
Shuichiro Kimura, Koreyoshi Imamura, Junichiro Futami
Biotechnology Progress 36 ( 5 ) e3031 2020.9
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Hidetaka Yokota, Miki Kadowaki, Tsutashi Matsuura, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Langmuir 36 ( 24 ) 6698 - 6705 2020.6
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Immobilization of surface non-affinitive protein onto a metal surface by an external electric field Reviewed
Olivier Tramis, Ryosuke Iizuka, Hajime Nakao, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Journal of Bioscience and Bioengineering 129 ( 3 ) 348 - 353 2020.3
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Picoliter enzyme reactor on a nanofluidic device exceeding the bulk reaction rate Reviewed
Koki Yamamoto, Kyojiro Morikawa, Hiroyuki Imanaka, Koreyoshi Imamura, Takehiko Kitamori
The Analyst 145 ( 17 ) 5801 - 5807 2020
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Pico-liter protein digestion and separation using nanofluidic device Reviewed
Kyojiro Morikawa, Koki Yamamoto, Hiroki Sano, Yutaka Kaoze, Hisashi Shimizu, Hiroyuki Imanaka, Koreyoshi Imamura, Takehiko Kitamori
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 1125 - 1126 2020
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Controlling the drying process in vacuum foam drying under low vacuum conditions by inducing foaming by needle stimulation of the solution Reviewed
Fumihiro Hidaka, Tomo Satoh, Akiho Fujioka, Koji Takeda, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Drying Technology 37 ( 12 ) 1520 - 1527 2019.9
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Physical Stability of an Amorphous Sugar Matrix Dried From Methanol as an Amorphous Solid Dispersion Carrier and the Influence of Heat Treatment Reviewed
Koji Takeda, Takanari Sekitoh, Akiho Fujioka,, Kayoko Yamamoto, Takashi Okamoto, Tsutashi Matsuura, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Journal of Pharmaceutical Sciences 108 ( 6 ) 2056 - 2062 2019.6
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Nanofluidic enzyme reactor exceeding limit of bulk reaction rate Reviewed
Koki Yamamoto, Kyojiro Morikawa, Koreyoshi Imamura, Hiroyuki Imanaka, Takehiko Kitamori
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 113 - 114 2019
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Adsorption characteristics of various proteins on a metal surface in the presence of an external electric potential Reviewed
Ei Ei Htwe, Yuhi Nakama, Yuko Yamamoto, Hiroshi Tanaka, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
Colloids and Surfaces B: Biointerfaces 166 262 - 268 2018.6
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Hydrophobic Attraction Measured between Asymmetric Hydrophobic Surfaces Reviewed
Naoyuki Ishida, Kohei Matsuo, Koreyoshi Imamura, Vincent S. J. Craig
Langmuir 34 ( 12 ) 3588 - 3596 2018.3
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Influence of an external electric field on removal of protein fouling on a stainless steel surface by proteolytic enzymes Reviewed
Ei Ei Htwe, Yuhi Nakama, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
COLLOIDS AND SURFACES B-BIOINTERFACES 159 118 - 124 2017.11
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Characteristics of proteinaceous additives in stabilizing enzymes during freeze-thawing and -drying Reviewed
Takanori Shimizu, Tamayo Korehisa, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 81 ( 4 ) 687 - 697 2017.4
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Surfactant-Free Solid Dispersions of Hydrophobic Drugs in an Amorphous Sugar Matrix Dried from an Organic Solvent Reviewed
Koji Takeda, Yuto Gotoda, Daichi Hirota, Fumihiro Hidaka, Tomo Sato, Tsutashi Matsuura, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
MOLECULAR PHARMACEUTICS 14 ( 3 ) 791 - 798 2017.3
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Adsorption of lysozyme on base metal surfaces in the presence of an external electric potential Reviewed
Ei Ei Htwe, Yuhi Nakama, Hiroshi Tanaka, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
COLLOIDS AND SURFACES B-BIOINTERFACES 147 9 - 16 2016.11
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Molecular design of proteinaceous cushion for sensitive biomolecular interaction detection system
Hiroyuki Imanaka, Koki Dare, Koreyoshi Imamura
NEW BIOTECHNOLOGY 33 S72 - S72 2016.7
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Surfactant-free solid dispersion of fat-soluble flavour in an amorphous sugar matrix Reviewed
Tomo Satoh, Fumihiro Hidaka, Kento Miyake, Natsuki Yoshiyama, Koji Takeda, Tsutashi Matsuura, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
FOOD CHEMISTRY 197 1136 - 1142 2016.4
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The use of a proteinaceous "cushion" with a polystyrene-binding peptide tag to control the orientation and function of a target peptide adsorbed to a hydrophilic polystyrene surface Reviewed
Hiroyuki Imanaka, Daisuke Yamadzumi, Keisuke Yanagita, Naoyuki Ishida, Kazuhiro Nakanishi, Koreyoshi Imamura
BIOTECHNOLOGY PROGRESS 32 ( 2 ) 527 - 534 2016.3
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Improvement of Biomolecular Interaction Detection Sensitivity through Molecular Design of Cushion Protein
Imanaka Hiroyuki, Date Koki, Matoba Haruka, Ishida Naoyuki, Imamura Koreyoshi
Abstract of annual meeting of the Surface Science of Japan 36 37 - 37 2016
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Effect of surface hydrophobicity on short-range hydrophobic attraction between silanated silica surfaces Reviewed
Yuhei Soga, Hiroyuki Imanaka, Koreyoshi Imamura, Naoyuki Ishida
ADVANCED POWDER TECHNOLOGY 26 ( 6 ) 1729 - 1733 2015.11
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Inhibitory effects of additives and heat treatment on the crystallization of freeze-dried sugar Reviewed
Kohshi Kinugawa, Mitsunori Kinuhata, Ryo Kagotani, Hiroyuki Imanaka, Naoyuki Ishida, Mizuki Kitamatsu, Kazuhiro Nakanishi, Koreyoshi Imamura
JOURNAL OF FOOD ENGINEERING 155 37 - 44 2015.6
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Influence of sugar surfactant structure on the encapsulation of oil droplets in an amorphous sugar matrix during freeze-drying Reviewed
Shota Nakayama, Yoshifumi Kimura, Sayuri Miki, Jun Oshitani, Takashi Kobayashi, Shuji Adachi, Tsutashi Matsuura, Hiroyuki Imanaka, Naoyuki Ishida, Hiroko Tada, Kazuhiro Nakanishi, Koreyoshi Imamura
FOOD RESEARCH INTERNATIONAL 70 143 - 149 2015.4
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Effect of Lipid Amount on Surfactant-free Solid Lipid Nanoparticle Formation by Hot Homogenization Reviewed
Jun Oshitani, Natsumi Murakami, Mikio Yoshida, Naoyuki Ishida, Koreyoshi Imamura, Hideki Ichikawa
CHEMISTRY LETTERS 43 ( 7 ) 1011 - 1013 2014.7
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Characteristics of Sugar Surfactants in Stabilizing Proteins During Freeze-Thawing and Freeze-Drying Reviewed
Koreyoshi Imamura, Katsuyuki Murai, Tamayo Korehisa, Noriyuki Shimizu, Ryo Yamahira, Tsutashi Matsuura, Hiroko Tada, Hiroyuki Imanaka, Naoyuki Ishida, Kazuhiro Nakanishi
JOURNAL OF PHARMACEUTICAL SCIENCES 103 ( 6 ) 1628 - 1637 2014.6
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Stabilization of Protein by Sugar Surfactant
IMAMURA Koreyoshi
Oleoscience 14 ( 11 ) 487 - 493 2014
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Effect of Surface Hydrophobicity on Short-Range Hydrophobic Attraction between Silanated Silica Surfaces Reviewed
Soga Yuuhei, Imanaka Hiroyuki, Imamura Koreyoshi, Ishida Naoyuki
Journal of the Society of Powder Technology, Japan 51 ( 5 ) 343 - 348 2014
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Shigemori Yojiro, Yoshida Kaori, Imamura Koreyoshi, Takahashi Yuichiro, Imanaka Hiroyuki
Seibutsu Butsuri 54 ( 1 ) S207 2014
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Matsushita Runa, Ishida Naoyuki, Imamura Koreyoshi, Imanaka Hiroyuki
Seibutsu Butsuri 54 ( 1 ) S209 2014
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Improving the physical stability of freeze-dried amorphous sugar matrices by compression at several hundreds MPa Reviewed
Ryo Kagotani, Kohshi Kinugawa, Mayo Nomura, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
JOURNAL OF PHARMACEUTICAL SCIENCES 102 ( 7 ) 2187 - 2197 2013.7
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Improvement on the freeze-thaw stability of corn starch gel by the polysaccharide from leaves of Corchorus olitorius L. Reviewed
Eiji Yamazaki, Toru Sago, Yoshiaki Kasubuchi, Kazuhito Imamura, Toshio Matsuoka, Osamu Kurita, Hironobu Nambu, Yasuki Matsumura
Carbohydrate Polymers 94 ( 1 ) 555 - 560 2013.4
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Characteristics of amorphous matrices composed of different types of sugars in encapsulating emulsion oil droplets during freeze-drying Reviewed
Koreyoshi Imamura, Yoshifumi Kimura, Shota Nakayama, Miki Sayuri, Seiji Ogawa, Tatsuya Hoshino, Jun Oshitani, Takashi Kobayashi, Shuji Adachi, Tsutashi Matsuura, Hiroyuki Imanaka, Naoyuki Ishida, Kazuhiro Nakanishi
FOOD RESEARCH INTERNATIONAL 51 ( 1 ) 201 - 207 2013.4
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On the preparation of indoxyl red from indican and some new characteristics Reviewed
Jingyuan Song, Mizuki Kitamatsu, Koreyoshi Imamura, Hitoshi Ohmori, Kouji Watanabe, Kazuhiro Nakanishi
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 23 ( 3 ) 627 - 629 2013.2
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The Discoidin Domain of Bacillus circulans beta-Galactosidase Plays an Essential Role in Repressing Galactooligosaccharide Production Reviewed
Jingyuan Song, Hiroyuki Imanaka, Koreyoshi Imamura, Masashi Minoda, Shotaro Yamaguchi, Kazuhiro Nakanishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77 ( 1 ) 73 - 79 2013.1
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Impact of compression, physical aging, and freezing rate on the crystallization characteristics of an amorphous sugar matrix Reviewed
Koreyoshi Imamura, Kohshi Kinugawa, Ryo Kagotani, Mayo Nomura, Kazuhiro Nakanishi
JOURNAL OF FOOD ENGINEERING 112 ( 4 ) 313 - 318 2012.10
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Heterogeneity of the state and functionality of water molecules sorbed in an amorphous sugar matrix Reviewed
Koreyoshi Imamura, Ryo Kagotani, Mayo Nomura, Kohshi Kinugawa, Kazuhiro Nakanishi
CARBOHYDRATE RESEARCH 351 108 - 113 2012.4
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Rod-shaped Hydroxyapatite Nanoparticle Formation by Maturation with AOT Reviewed
Jun Oshitani, Nobuhide Kataoka, Mikio Yoshida, Kuniaki Gotoh, Koreyoshi Imamura
CHEMISTRY LETTERS 40 ( 10 ) 1085 - 1086 2011.10
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Cloning and Expression of a beta-Galactosidase Gene of Bacillus circulans Reviewed
Jingyuan Song, Hiroyuki Imanaka, Koreyoshi Imamura, Masashi Minoda, Toru Katase, Yukiko Hoshi, Shotaro Yamaguchi, Kazuhiro Nakanishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 75 ( 6 ) 1194 - 1197 2011.6
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Rod-shaped Hydroxyapatite Nanoparticle Formation Using AOT and Water without Oil Reviewed
Jun Oshitani, Nobuhide Kataoka, Mikio Yoshida, Kuniaki Gotoh, Koreyoshi Imamura, Hidekazu Tanaka
CHEMISTRY LETTERS 40 ( 4 ) 400 - 401 2011.4
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Influence of compression on water sorption, glass transition, and enthalpy relaxation behavior of freeze-dried amorphous sugar matrices Reviewed
Koreyoshi Imamura, Ryo Kagotani, Mayo Nomura, Kazuhiro Tanaka, Kohshi Kinugawa, Kazuhiro Nakanishi
INTERNATIONAL JOURNAL OF PHARMACEUTICS 408 ( 1-2 ) 76 - 83 2011.4
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Causes of the Production of Multiple Forms of beta-Galactosidase by Bacillus circulans Reviewed
Jingyuan Song, Kiriko Abe, Hiroyuki Imanaka, Koreyoshi Imamura, Masashi Minoda, Shotaro Yamaguchi, Kazuhiro Nakanishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 75 ( 2 ) 268 - 278 2011.2
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Multiple States of Sorbed Water Molecules in Amorphous Sugar Matrix andtheir Different Impacts on Physical Properties of the Matrix Invited Reviewed
Koreyoshi Imamura
japan journal of food engineering 12 ( 1 ) 1 - 9 2011
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Water Sorption, Glass Transition, and Protein-Stabilizing Behavior of an Amorphous Sucrose Matrix Combined With Various Materials Reviewed
Koreyoshi Imamura, Toru Yokoyama, Atsushi Fukushima, Mitsunori Kinuhata, Kazuhiro Nakanishi
JOURNAL OF PHARMACEUTICAL SCIENCES 99 ( 11 ) 4669 - 4677 2010.11
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Development of a highly efficient indigo dyeing method using indican with an immobilized beta-glucosidase from Aspergillus niger Reviewed
Jingyuan Song, Hiroyuki Imanaka, Koreyoshi Imamura, Kouichi Kajitani, Kazuhiro Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 110 ( 3 ) 281 - 287 2010.9
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Influences of properties of protein and adsorption surface on removal kinetics of protein adsorbed on metal surface by H2O2-electrolysis treatment Reviewed
Koreyoshi Imamura, Manabu Oshita, Masumi Iwai, Toshiki Kuroda, Ippei Watanabe, Takaharu Sakiyama, Kazuhiro Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 345 ( 2 ) 474 - 480 2010.5
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Cultivation characteristics and gene expression profiles of Aspergillus oryzae by membrane-surface liquid culture, shaking-flask culture, and agar-plate culture Reviewed
Hiroyuki Imanaka, Soukichi Tanaka, Bin Feng, Koreyoshi Imamura, Kazuhiro Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 109 ( 3 ) 267 - 273 2010.3
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Impacts of Compression on Crystallization Behavior of Freeze-Dried Amorphous Sucrose Reviewed
Koreyoshi Imamura, Mayo Nomura, Kazuhiro Tanaka, Nobuhide Kataoka, Jun Oshitani, Hiroyuki Imanaka, Kazuhiro Nakanishi
JOURNAL OF PHARMACEUTICAL SCIENCES 99 ( 3 ) 1452 - 1463 2010.3
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低温度領域における糖類アモルファスマトリクスのタンパク質安定化作用と糖分子の束縛状態
今村 維克, 横山 徹, 大山 健一, 絹畠 光倫, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2010 584 - 584 2010
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各種糖類アモルファスマトリクスに収着した水の状態とガラス転移温度との関係
籠谷 亮, 衣川 耕史, 野村 真世, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2010 583 - 583 2010
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Streptmyces mobaraensis由来epsilon-Lysine acylaseによるN-epsilon-Lauroyl-L-Lysineの効率的合成
内田 達也, 田口 友造, 今中 洋行, 今村 維克, 是石 真友子, 中西 一弘
化学工学会 研究発表講演要旨集 2010 1003 - 1003 2010
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國方 俊暢, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2010 997 - 997 2010
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オートトランスポーターを用いたペプチド表層提示系の検討
今中 洋行, 植田 久子, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2010 165 - 165 2010
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Development of peptide immobilization method using PS-tagged cushion protein
Hiroyuki Imanaka, Daisuke Yamazumi, Toshinobu Kunikata, Koreyoshi Imamura, Kazuhiro Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 108 S161 - S161 2009.11
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Formation characteristics of calcium phosphate deposits on a metal surface by H2O2-electrolysis reaction under various conditions Reviewed
Kazuaki Kanamoto, Koreyoshi Imamura, Nobuhide Kataoka, Jun Oshitani, Hiroyuki Imanaka, Kazuhiro Nakanishi
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 350 ( 1-3 ) 79 - 86 2009.10
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Temperature Scanning FTIR Analysis of Secondary Structures of Proteins Embedded in Amorphous Sugar Matrix Reviewed
Koreyoshi Imamura, Ken-Ichi Ohyama, Toru Yokoyama, Yoshinobu Maruyama, Nakanishi Kazuhiro
JOURNAL OF PHARMACEUTICAL SCIENCES 98 ( 9 ) 3088 - 3098 2009.9
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Purification, Characterization, Molecular Cloning, and Expression of a New Aminoacylase from Streptomyces mobaraensis That Can Hydrolyze N-(Middle/Long)-chain-fatty-acyl-L-amino Acids as Well as N-Short-chain-acyl-L-amino acids Reviewed
Mayuko Koreishi, Yasuyuki Nakatani, Manami Ooi, Hiroyuki Imanaka, Koreyoshi Imamura, Kazuhiro Nakanishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 73 ( 9 ) 1940 - 1947 2009.9
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Efficient N epsilon-lauroyl-L-lysine production by recombinant epsilon-lysine acylase from Streptomyces mobaraensis Reviewed
Mayuko Koreishi, Ryoko Kawasaki, Hiroyuki Imanaka, Koreyoshi Imamura, Yasuaki Takakura, Kazuhiro Nakanishi
JOURNAL OF BIOTECHNOLOGY 141 ( 3-4 ) 160 - 165 2009.5
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糖類アモルファス材料の水分収着特性とタンパク質安定化作用に及ぼす高圧処理の影響
今村 維克, 野村 真世, 木村 佳文, 伊久 珠代, 絹畠 光倫, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2009 779 - 779 2009
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クッションタンパク質を利用したバイオ分子固定化技術の開発と応用
麻那古 秀友, 山隅 大輔, 大塚 隆尚, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2009 1060 - 1060 2009
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PS-tag連結タンパク質を利用したバイオ分子間相互作用解析
今中 洋行, 上崎 英範, 國方 俊暢, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2009 934 - 934 2009
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糖類アモルファスマトリクスにおける糖-高分子間相互作用の形成特性
今村 維克, 横山 徹, 大山 健一, 野村 真世, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2009 608 - 608 2009
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今中 洋行, 植田 久子, 山下 麻衣, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2009 606 - 606 2009
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Recent advances in controlled immobilization of proteins onto the surface of the solid substrate and its possible application to proteomics Reviewed
Kazuhiro Nakanishi, Takaharu Sakiyama, Yoichi Kumada, Koreyoshi Imamura, Hiroyuki Imanaka
Current Proteomics 5 ( 3 ) 161 - 175 2008.10
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Adsorption Characteristics of Various Proteins to a Titanium Surface Reviewed
Koreyoshi Imamura, Miyuki Shimomura, Shungo Nagai, Megumi Akamatsu, Kazuhiro Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 106 ( 3 ) 273 - 278 2008.9
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True density analysis of a freeze-dried amorphous sugar matrix Reviewed
Koreyoshi Imamura, Yoshinobu Maruyama, Kazuhiro Tanaka, Tohru Yokoyama, Hiroyuki Imanaka, Kazuhiro Nakanishi
JOURNAL OF PHARMACEUTICAL SCIENCES 97 ( 7 ) 2789 - 2797 2008.7
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Characteristics of hydrogen bond formation between sugar and polymer in freeze-dried mixtures under different rehumidification conditions and its impact on the glass transition temperature Reviewed
Koreyoshi Imamura, Yoko Asano, Yoshinobu Maruyama, Tohru Yokoyama, Mayo Nomura, Seiji Ogawa, Kazuhiro Nakanishi
JOURNAL OF PHARMACEUTICAL SCIENCES 97 ( 3 ) 1301 - 1312 2008.3
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Temperature scanning FTIR analysis of interactions between sugar and polymer additive in amorphous sugar-polymer mixtures Reviewed
Koreyoshi Imamura, Ken-Ichi Ohyama, Toru Yokoyama, Yoshinobu Maruyama, Hiroyuki Imanaka, Kazuhiro Nakanishi
JOURNAL OF PHARMACEUTICAL SCIENCES 97 ( 1 ) 519 - 528 2008.1
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Fourier self-deconvolution analysis of hydrogen bonding states of polyvinylpyrrolidone in an amorphous sugar matrix below and above the glass transition temperature Reviewed
Koreyoshi Imamura, Ken-ichi Ohyama, Kazuko Tani, Toru Yokoyama, Yoshinobu Maruyama, Hiroyuki Imanaka, Kazuhiro Nakanishi
SPECTROSCOPY LETTERS 41 ( 6 ) 305 - 312 2008
-
Factors Affecting Performance of Cleaning Technique for Metal Surfaces Based on Electrolysi of Hydrogen Peroxide, H2O2-electrolysis Invited Reviewed
IMAMURA Koreyoshi
NIHON SHOKUHIN KOUGAKUKAISHI (Japan Journal of Food Engineering) 9 ( 4 ) 229 - 238 2008
-
今村 維克, 野村 真世, 横山 徹, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2008 339 - 339 2008
-
田中 創吉, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2008 378 - 378 2008
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親和性ペプチドを利用したポリスチレンおよびガラス表面への蛋白質固定化とその利用
今中 洋行, 上崎 英範, 石村 遼太, 熊田 陽一, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2008 345 - 345 2008
-
山隅 大輔, 柳田 圭介, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2008 418 - 418 2008
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糖類アモルファスマトリクスの包括安定化作用および物理的諸特性に及ぼす高圧処理の影響
今村 維克, 野村 真世, 田中 一宏, 木村 佳文, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2008 175 - 175 2008
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今中 洋行, 前川 真光, 今村 維克, 近藤 英作, 中西 一弘
化学工学会 研究発表講演要旨集 2008 955 - 955 2008
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TPR domain of Ser/Thr phosphatase of Aspergillus oryzae shows no auto-inhibitory effect on the dephosphorylation activity Reviewed
Bin Feng, Chun-Hui Zhao, Soukichi Tanaka, Hiroyuki Imanaka, Koreyoshi Imamura, Kazuhiro Nakanishi
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 41 ( 3 ) 281 - 285 2007.8
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Enzymatic synthesis of beta-lactam antibiotics and N-fatty-acylated amino compounds by the acyl-transfer reaction catalyzed by penicillin V acylase from Streptomyces mobaraensis Reviewed
Mayuko Koreishi, Kazuha Tani, Yuuichi Ise, Hiroyuki Imanaka, Koreyoshi Imamura, Kazuhiro Nakanishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 71 ( 6 ) 1582 - 1586 2007.6
-
Cloning and characterization of penicillin V acylase from Streptomyces mobaraensis Reviewed
Demin Zhang, Mayuko Koreishi, Hiroyuki Imanaka, Koreyoshi Imamura, Kazuhiro Nakanishi
JOURNAL OF BIOTECHNOLOGY 128 ( 4 ) 788 - 800 2007.3
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Protein-protein interaction analysis using an affinity peptide tag and hydrophilic polystyrene plate Reviewed
Yoichi Kumada, Chunhui Zhao, Ryota Ishimura, Hiroyuki Imanaka, Koreyoshi Imamura, Kazuhiro Nakanishi
JOURNAL OF BIOTECHNOLOGY 128 ( 2 ) 354 - 361 2007.2
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Adsorption characteristics of oligopeptides composed of acidic and basic amino acids on titanium surface Reviewed
Koreyoshi Imamura, Yuuki Kawasaki, Takeshi Nagayasu, Takaharu Sakiyama, Kazuhiro Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 103 ( 1 ) 7 - 12 2007.1
-
Development of a one-step ELISA method using an affinity peptide tag specific to a hydrophilic polystyrene surface Reviewed
Yoichi Kumada, Shigeo Katoh, Hiroyuki Imartaka, Koreyoshi Imamura, Kazuhiro Nakanishi
JOURNAL OF BIOTECHNOLOGY 127 ( 2 ) 288 - 299 2007.1
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オートトランスポーターを利用した異種タンパク質の大腸菌細胞表層提示系の構築
今中 洋行, 山下 麻衣, 盛永 鈴香, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2007 417 - 417 2007
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熊田 陽一, Zhao Chunhui, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2007 427 - 427 2007
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糖-高分子複合アモルファスマトリクスにおける分子間相互作用と物理的安定性
今村 維克, 浅野 容子, 大山 健一, 丸山 佳伸, 崎山 高明, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2007 368 - 368 2007
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過酸化水素-電気分解における金属表面でのハイドロキシアパタイト相の形成とその特性評価
金本 和明, 今村 維克, 片岡 信秀, 押谷 潤, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2007 126 - 126 2007
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H2O2-電気分解による金属表面および水溶液中の有機物質の分解・除去
今村 維克, 黒田 俊樹, 小崎 正晴, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2007 702 - 702 2007
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オートトランスポーターを用いた大腸菌細胞表層提示系に及ぼすシグナルペプチドの影響
今中 洋行, 辰本 渉, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2007 842 - 842 2007
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大腸菌由来Cysteine synthaseの複合体形成特性
今中 洋行, 森賀 雄大, 趙 春暉, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2007 841 - 841 2007
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過酸化水素-電気分解により生成したハイドロキシアパタイトの特性評価および骨芽様細胞の増殖・分化特性
今村 維克, 金本 和明, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2007 990 - 990 2007
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Identification of genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture Reviewed
Bin Feng, Masakazu Morita, Hiroyuki Imanaka, Koreyoshi Imamura, Kazuhlro Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 102 ( 5 ) 470 - 473 2006.11
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Effects of the supporting electrolyte on the kinetics of the removal of proteins adsorbed on a stainless steel surface by H2O2-electrolysis Reviewed
Koreyoshi Imamura, Ippei Watanabe, Takaharu Sakiyama, Kazuhiro Nakanishi
LANGMUIR 22 ( 16 ) 7035 - 7040 2006.8
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Temperature scanning FTIR analysis of hydrogen bonding states of various saccharides in amorphous matrixes below and above their glass transition temperatures Reviewed
Koreyoshi Imamura, Keisuke Sakaura, Ken-ichi Ohyama, Atsushi Fukushima, Hiroyuki Imanaka, Takaharu Sakiyama, Kazuhiro Nakanishi
JOURNAL OF PHYSICAL CHEMISTRY B 110 ( 31 ) 15094 - 15099 2006.8
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Cloning, overexpression, purification, and characterization of O-acetylserine sulfhydrylase-B from Escherichia coli Reviewed
C Zhao, Y Kumada, H Imanaka, K Imamura, K Nakanishi
PROTEIN EXPRESSION AND PURIFICATION 47 ( 2 ) 607 - 613 2006.6
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Protease susceptibility of beta-lactoglobulin adsorbed on stainless steel surface as evidence of contribution of its specific segment to adsorption Reviewed
Takaharu Sakiyama, Atsuko Aya, Mari Embutsu, Koreyoshi Imamura, Kazuhiro Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 101 ( 5 ) 434 - 439 2006.5
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Screening and characterization of affinity peptide tags specific to polystyrene supports for the orientated immobilization of proteins Reviewed
Y Kumada, Y Tokunaga, H Imanaka, K Imamura, T Sakiyama, S Katoh, K Nakanishi
BIOTECHNOLOGY PROGRESS 22 ( 2 ) 401 - 405 2006.3
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On the interaction site of serine acetyltransferase in the cysteine synthase complex from Escherichia coli Reviewed
CH Zhao, Y Moriga, B Feng, Y Kumada, H Imanaka, K Imamura, K Nakanishi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 341 ( 4 ) 911 - 916 2006.3
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A novel acylase from Streptomyces mobaraensis that efficiently catalyzes hydrolysis/synthesis of capsaicins as well as N-Acyl-L-amino acids and N-acyl-peptides Reviewed
M Koreishi, DM Zhang, H Imanaka, K Imamura, S Adachi, R Matsuno, K Nakanishi
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 54 ( 1 ) 72 - 78 2006.1
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今村 維克, 丸山 佳伸, 横山 徹, 大山 健一, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2006 413 - 413 2006
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ポリスチレン親和性ペプチドを利用したタンパク質間相互作用の分析
熊田 陽一, 石村 遼太, 上崎 英範, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2006 996 - 996 2006
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柳田 圭介, 中泉 雅人, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2006 1002 - 1002 2006
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放線菌 Streptomyces mobaraensis 由来アシラーゼによる転移反応とその応用
是石 真友子, 谷 和葉, 伊勢 雄一, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2006 949 - 949 2006
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高度な物理的安定性とタンパク質安定化作用を兼ね備えた糖類アモルファスマトリクスの創製
横山 徹, 丸山 佳伸, 大山 健一, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2006 995 - 995 2006
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Purification and characterization of a novel aminoacylase from Streptomyces mobaraensis Reviewed
M Koreishi, F Asayama, H Imanaka, K Imamura, M Kadota, T Tsuno, K Nakanishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 69 ( 10 ) 1914 - 1922 2005.10
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A novel epsilon-lysine acylase from Streptomyces mobaraensis for synthesis of N epsilon-acyl-L-lysines Reviewed
M Koreishi, R Kawasaki, H Imanaka, K Imamura, K Nakanishi
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY 82 ( 9 ) 631 - 637 2005.9
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Adsorption characteristics of various organic substances on the surfaces of tantalum, titanium, and zirconium Reviewed
T Nagayasu, K Imamura, K Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 286 ( 2 ) 462 - 470 2005.6
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Zhao Chunhui, Imanaka Hiroyuki, Imamura Koreyoshi, Nakanishi Kazuhiro
2005 271 - 271 2005
-
金属表面に吸着した各種タンパク質のH2O2-電気分解処理における脱離特性と脱離促進因子
今村 維克, 尾下 学, 岩井 真澄, 金本 知明, 今中 洋行, 中西 一弘
化学工学会 研究発表講演要旨集 2005 294 - 294 2005
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放線菌 Streptomyces mobaraensis 由来新規アシラーゼの精製・特性解析および合成反応
是石 真友子, 川崎 涼子, 今中 洋行, 今村 維克, 中西 一弘, 丹尾 式希
化学工学会 研究発表講演要旨集 2005 270 - 270 2005
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膜面液体培養法を用いた遺伝子組換え麹カビによるNuclease S1生産
森田 正和, 安達 昇, 奥村 敦, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2005 242 - 242 2005
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温度走査フーリエ変換赤外分光分析による糖-高分子複合アモルファスマトリクスにおける分子間相互作用の解析
大山 健一, 谷 加寿子, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2005 293 - 293 2005
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長安 武司, 今中 洋行, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2005 292 - 292 2005
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ポリスチレン親和性ペプチド融合タンパク質の吸着特性とその応用
徳永 安秀, 熊田 陽一, 今石 大輔, 今中 洋行, 今村 維克, 崎山 高明, 中西 一弘
化学工学会 研究発表講演要旨集 2005f 311 - 311 2005
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Analysis of Differential Gene Expression of Aspergillus oryzae in Membrane-Surface Liquid Culture and Shaking Flask Culture by Subtractive Hybridization
Feng Bin
2005f 294 - 294 2005
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2'-デオキシアデノシン5'-三リン酸(dATP)の酵素合成に関する反応工学的研究
今中 洋行, 矢野 慎太郎, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2005f 214 - 214 2005
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Characteristics of alpha-glucosidase production from recombinant Aspergillus oryzae by membrane-surface liquid culture in comparison with various cultivation methods Reviewed
M Morita, H Shimamura, N Ishida, K Imamura, T Sakiyama, K Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 98 ( 3 ) 200 - 206 2004.9
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Production of nonproteinaceous amino acids using recombinant Escherichia coli cells expressing cysteine synthase and related enzymes with or without the secretion of O-acetyl-L-serine Reviewed
CH Zhao, K Ohno, K Sogoh, K Imamura, T Sakiyama, K Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 97 ( 5 ) 322 - 328 2004.5
-
Factors Affecting Performance of Cleaning Technique for Metal Surfaces Based on Electrolysis of Hydrogen Peroxide, H2O2-electrolysis Invited Reviewed
Koreyoshi Imamura
nihon shokuhin kougakukaishi (japan journal of food engineering) 9 ( 4 ) 229 - 238 2004
-
Production of Non-Proteinaceous Amino Acids using Recombinant E. coli Cells Reviewed
Nakanishi Kazuhiro, Zhao Chunhui, Imamura Koreyoshi, Ohno Katsuhiro
Asian Pacific Confederation of Chemical Engineering congress program and abstracts 2004 815 - 815 2004
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Removal Behavior of Protein Adsorbed on Metal Surface by H2O2-Electrolysis Treatment Reviewed
Imamura Koreyoshi, Watanabe Ippei, Aramoto Yumi, Sakiyama Takaharu, Nakanishi Kazuhiro
Asian Pacific Confederation of Chemical Engineering congress program and abstracts 2004 353 - 353 2004
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Effects of water activity and lipid addition on secondary structure of zein in powder systems Reviewed
Y Mizutani, Y Matsumura, K Imamura, K Nakanishi, T Mori
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 51 ( 1 ) 229 - 235 2003.1
-
温度走査FTIRによる各種糖類アモルファスマトリクスにおける相互作用の解析
今村 維克, 坂浦 啓介, 福島 厚志, 大山 健一, 谷 加寿子, 崎山 高明, 中西 一弘
化学工学会 研究発表講演要旨集 2003 688 - 688 2003
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ステンレス表面上の耐熱性アミノペプチダーゼの付着特性と活性発現
長安 武司, 山越 憲吾, 吉岡 千里, 松本 訓智, 大西 宏輔, 今村 維克, 崎山 高明, 中西 一弘
化学工学会 研究発表講演要旨集 2003 682 - 682 2003
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膜面液体培養法(MSLC)と振盪フラスコ培養(SFC)を用いたα-glucosidase生産性の差についての考察
森田 正和, 今村 維克, 崎山 高明, 中西 一弘
化学工学会 研究発表講演要旨集 2003 681 - 681 2003
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天然由来の高分子電解質を素材とした複合ゲルの調製とその膨潤特性
崎山 高明, 今村 維克, 中西 一弘
化学工学会 研究発表講演要旨集 2003 995 - 995 2003
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Isolation of a Novel Acylase from Streptomyces mobaraensis for Synthesis of Useful Amide Compounds
Zhang Demin, Koreishi Mayuko, Imamura Koreyoshi, Sakiyama Takaharu, NAKANISHI Kazuhiro
化学工学会 研究発表講演要旨集 2003 136 - 136 2003
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ZHAO Chunhui, OHNO Katsuhiro, SOGOH Kohji, IMAMURA Koreyoshi, SAKIYAMA Takaharu, NAKANISHI Kazuhiro
化学工学会 研究発表講演要旨集 2003 685 - 685 2003
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Enzymatic synthesis of N-acyl-L-amino acids in a glycerol-water system using acylase I from pig kidney Reviewed
E Wada, M Handa, K Imamura, T Sakiyama, S Adachi, R Matsuno, K Nakanishi
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY 79 ( 1 ) 41 - 46 2002.1
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Enzymatic modification of beta-lactoglobulin with N-fatty-acyl-dipeptide by transglutaminase from Streptomyces mobaraense Reviewed
E Wada, H Masuda, K Imamura, T Sakiyama, S Adachi, R Matsuno, K Nakanishi
BIOTECHNOLOGY LETTERS 23 ( 17 ) 1367 - 1372 2001.9
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Role of sucrose-LDH hydrogen bond for thermal stabilizing effect of sucrose on freeze-dried LDH Reviewed
T Suzuki, K Imamura, H Fujimoto, M Okazaki
DRYING TECHNOLOGY 17 ( 7-8 ) 1429 - 1439 1999
-
Relation between thermal stabilizing effect of sucrose on LDH and sucrose-LDH hydrogen bond Reviewed
T Suzuki, K Imamura, H Fujimoto, M Okazaki
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 31 ( 4 ) 565 - 570 1998.8
-
Thermal stabilization of freeze-dried enzymes by sugars Reviewed
T Suzuki, K Imamura, K Yamamoto, T Satoh, M Okazaki
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 30 ( 4 ) 609 - 613 1997.8
-
Stabilizing effect on freeze-dried proteins by amorphous matrices of sugars Reviewed
T Suzuki, K Imamura, K Yamamoto, M Okazaki
DRYING '96, VOL B 1261 - 1266 1996
-
A New Method for Measurement of Powder Characteristics Based on Reentrainment Phenomena Reviewed
Hiroaki Masuda, Shuji Matsusaka, Koreyoshi Imamura
KONA Powder and Particle Journal 12 133 - 143 1994
-
A New Method for the Measurement of Powder Characteristics Based on the Reentrainment Phenomena.
MASUDA Hiroaki, MATSUSAKA Shuji, IMAMURA Koreyoshi
Journal of the Research Association of Powder Technology, Japan 29 ( 12 ) 897 - 905 1992
MISC
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パッキングのコントロールにより,難結晶性蛋白質の結晶化を促進する結晶化法
小坂恵, 山田秀徳, 二見淳一郎, 多田宏子, 今村維克, 玉田太郎
日本細胞生物学会大会(Web) 71st 2019
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パッキング相互作用に着目したタンパク質結晶化法
小坂恵, 山田秀徳, 二見淳一郎, 多田宏子, 今村維克, 玉田太郎
日本結晶学会年会講演要旨集 2019 2019
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難結晶性蛋白質のパッキングをコントロールする結晶化法の開発と評価
小坂恵, 山田秀徳, 二見淳一郎, 多田宏子, 今村維克, 玉田太郎
日本結晶学会年会講演要旨集 2018 2018
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小坂恵, 山田秀徳, 二見淳一郎, 多田宏子, 今村維克, 玉田太郎
日本結晶学会年会講演要旨集 2017 107 2017.11
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小坂恵, 山田秀徳, 二見淳一郎, 多田宏子, 今村維克, 玉田太郎
日本生化学会大会(Web) 90th ROMBUNNO.3P‐0100 (WEB ONLY) 2017
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Sugar surfactantの応用 : 油滴包括およびタンパク質安定化
今村 維克, 石田 尚之, 今中 洋行
日本食品工学会誌 = Japan journal of food engineering 17 ( 3 ) A - 7-11 2016.9
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( 28 ) 67 - 76 2015
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Matoba Haruka, Ishida Naoyuki, Imamura Koreyoshi, Imanaka Hiroyuki
67 190 - 190 2015
-
Abo Hirotaka, Hara Hirofumi, Umetsu Mitsuo, Ishida Naoyuki, Imamura Koreyoshi, Imanaka Hiroyuki
67 319 - 319 2015
-
標的遺伝子の5’末端周辺配列が大腸菌発現系に及ぼす影響
安保紘高, 原啓文, 石田尚之, 今村維克, 今中洋行
日本農芸化学会大会講演要旨集(Web) 2015 2015
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Abo Hirotaka, Miki Syunya, Hara Hirofumi, Yamazoe Atsushi, Hosoyama Akira, Tsuchikane Keiko, Imamura Koreyoshi, Imanaka Hiroyuki
66 111 - 111 2014
-
Utilization of Hydrophilic Polystyrene Affinity Peptide for Functional Biomolecular Immobilization and Effective Site Specific Peptide Binder Screening
H. Imanaka, R. Matsushita, T. Takimoto, T. Kunikata, K. Imamura, K. Nakanishi
BIOPOLYMERS 100 ( 3 ) 270 - 270 2013.5
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3P-180 Screening of novel gold affinity peptide and its application on protein immobilization
Shigemori Yojiro, Ishida Naoyuki, Imamura Koreyoshi, Takahashi Yuichiro, Imanaka Hiroyuki
65 233 - 233 2013
-
Matsushita Runa, Ishida Naoyuki, Imamura Koreyoshi, Imanaka Hiroyuki
65 123 - 123 2013
-
Kawasaki Kazuki, Matsumoto Kohei, Imanaka Hiroyuki, Imamura Koreyoshi, Kondou Eisaku
64 195 - 195 2012
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4Cp02 Screening of NFkB(p50) affinity peptides by adopting functional biomoleculer immobilization
Takimoto Takayuki, Miyahara Tetsuya, Imamura Koreyosi, Imanaka Hiroyuki, Kondou Eisaku, Nakanishi Kazuhiro
64 195 - 195 2012
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Takimoto Takayuki, Miyahara Tetsuya, Imanaka Hiroyuki, Imamura Koreyoshi, Kondo Eisaku, Nakanishi Kazuhiro
63 145 - 145 2011
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Imanaka Hiroyuki, Taguchi Yuzo, Uchida Tatsuya, Kumon Kazuki, Kuroki Kentaro, Imamura Koreyoshi, Nakanishi Kazuhiro
63 58 - 58 2011
-
Development of a highly efficient indigo dyeing method using indican with an immobilized beta-glucosidase from Aspergillus niger
Jingyuan Song, Hiroyuki Imanaka, Koreyoshi Imamura, Kouichi Kajitani, Kazuhiro Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 110 ( 3 ) 281 - 287 2010.9
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IMAMURA Koreyoshi
Calorimetry and thermal analysis. 37 ( 4 ) 154 - 161 2010.8
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Imanaka Hiroyuki, Tanaka Soukichi, Feng Bin, Imamura Koreyoshi, Nakanishi Kazuhiro
Memoirs of the Faculty of Engineering, Okayama University 44 73 - 83 2010.1
-
IMANAKA Hiroyuki, UEDA Hisako, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
22 34 - 34 2010
-
3P-1055 Multiformity of β-Galactosidase Produced from Bacillus circulans :
SONG Jingyuan, IMANAKA Hiroyuki, IMAMURA Koreyoshi, MINODA Masashi, YAMAGUCHI Shoutaro, NAKANISHI Kazuhiro
22 71 - 71 2010
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SHIMIZU Yuki, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
22 79 - 79 2010
-
MN-P23 Development of peptide immobilization method using PS-tagged cushion protein(Section X Micro/Nano Technology for Analysis and Cell Manipulation)
Imanaka Hiroyuki, Yamazumi Daisuke, Kunikata Toshinobu, Imamura Koreyoshi, Nakanishi Kazuhiro
Journal of bioscience and bioengineering 108 ( 1 ) S161 2009.11
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Efficient Ethanol Production from Wheat Bran by Enzymatic Saccharification Using Commercially Available Enzyme Products and Fermentation Using Bakers' Yeast
KOREISHI Mayuko, IMANAKA Hiroyuki, IMAMURA Koreyoshi, KARIYAMA Masahiro, NAKANISHI Kazuhiro
Seibutsu-kougaku Kaishi 87 ( 5 ) 216 - 223 2009.5
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2Ea05 Investigation of peptide surface display system by autotransporter
UEDA Hisako, YAMASHITA Mai, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
21 59 - 59 2009
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2Ip05 Investigation of Peptide-Protein Interaction Detection System by Cushion Protein
IMANAKA Hiroyuki, KUNIKATA Toshinobu, YAMANOUCHI Mai, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
21 126 - 126 2009
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2Da01 Construction of expression systems of a novel aminoacylase from Streptomyces mobaraensis
OOI Manami, NAKATANI Yasuyuki, KOREISHI Mayuko, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
21 44 - 44 2009
-
3Ga07 Development of rapid and sensitive detection system of human FOXP3-DNA interaction
MAEKAWA Masami, IMANAKA Hiroyuki, IMAMURA Koreyoshi, KONDO Eisaku, NAKANISHI Kazuhiro
20 186 - 186 2008
-
TERAKAWA Shuji, MORI Masashi, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
20 59 - 59 2008
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3Da04 Immobilization of Biomolecules by Cushion Protein on Solid Surfaces
IMANAKA Hiroyuki, YAMAZUMI Daisuke, YANAGITA Keisuke, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
20 126 - 126 2008
-
Annual report of study for food under the supporting program by The Skylark Food Science Institute ( 21 ) 109 - 115 2008
-
Enzymatic synthesis of beta-lactam antibiotics and N-fatty-acylated amino compounds by the acyl-transfer reaction catalyzed by penicillin V acylase from Streptomyces mobaraensis
Mayuko Koreishi, Kazuha Tani, Yuuichi Ise, Hiroyuki Imanaka, Koreyoshi Imamura, Kazuhiro Nakanishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 71 ( 6 ) 1582 - 1586 2007.6
-
KOREISHI Mayuko, ISE Yuuichi, TANI Kazuha, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
19 79 - 79 2007
-
TANAKA Soukichi, FENG Bin, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
19 141 - 141 2007
-
NAKATANI Yasuyuki, KOREISHI Mayuko, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
19 79 - 79 2007
-
TATSUMOTO Wataru, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro
19 77 - 77 2007
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ポリスチレン親和性ペプチド標識タンパク質を用いる迅速免疫測定
中西一弘, 熊田陽一, 今中洋行, 今村維克
日本食品工学会年次大会講演要旨集 8th 2007
-
Stabilization of Proteins during Freeze-drying and Storage by "Sugar Glass"
IMAMURA Koreyoshi
81 ( 949 ) 908 - 912 2006.11
-
Protease susceptibility of beta-lactoglobulin adsorbed on stainless steel surface as evidence of contribution of its specific segment to adsorption
Takaharu Sakiyama, Atsuko Aya, Mari Embutsu, Koreyoshi Imamura, Kazuhiro Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 101 ( 5 ) 434 - 439 2006.5
-
ポリスチレン親和性ペプチド標識タンパク質を用いる迅速免疫測定
熊田陽一, 今中洋行, 今村維克, 加藤滋雄, 中西一弘
化学工学会年会研究発表講演要旨集 71st 2006
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ポリスチレン表面に親和性を示すペプチドタグを付加した酵素の設計と特性解析
今石大輔, 熊田陽一, 今中洋行, 今村維克, 崎山高明, 中西一弘
日本農芸化学会中四国支部講演会講演要旨集 14th (Web) 2006
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Isolation and Characterization of a Thermostable Monoacylglycerol Lipase from Soil Bacterium
Hiroyuki Imanaka, Masashi Mori, Koreyoshi Imamura, Takaharu Sakiyama, Kazuhiro Nakanishi
Proceedings for Asia-Pacific Biochemical Engineering Conference (APBioChEC’05) 2005
-
2E11-4 Orientated immobilization of protein by use of affinity peptide specific to polystyrene
KUMADA Yoichi, IMANAKA Hiroyuki, IMAMURA Koreyoshi, KATOH Shigeo, SAKIYAMA Takaaki, NAKANISHI Kazuhiro
17 153 - 153 2005
-
IMAMURA Koreyoshi
Cryobiology and cryotechnology 51 ( 1 ) 31 - 35 2005
-
ポリスチレン親和性ペプチド融合タンパク質の吸着特性とその応用
徳永安秀, 熊田陽一, 今石大輔, 今中洋行, 今村維克, 崎山高明, 中西一弘
化学工学会秋季大会研究発表講演要旨集(CD-ROM) 37th 2005
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Effects of carboxyl groups on the adsorption behavior of low-molecular-weight substances on a stainless steel surface
T Nagayasu, C Yoshioka, K Imamura, K Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 279 ( 2 ) 296 - 306 2004.11
-
Characteristics of alpha-glucosidase production from recombinant Aspergillus oryzae by membrane-surface liquid culture in comparison with various cultivation methods
M Morita, H Shimamura, N Ishida, K Imamura, T Sakiyama, K Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 98 ( 3 ) 200 - 206 2004.9
-
Use of a novel affinity tag selected with a bacterial random peptide library for improving activity retention of glutathione S-transferase adsorbed on a polystyrene surface
T Sakiyama, S Ueno, K Imamura, K Nakanishi
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 28 ( 4-6 ) 207 - 214 2004.6
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Production of nonproteinaceous amino acids using recombinant Escherichia coli cells expressing cysteine synthase and related enzymes with or without the secretion of O-acetyl-L-serine
CH Zhao, K Ohno, K Sogoh, K Imamura, T Sakiyama, K Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 97 ( 5 ) 322 - 328 2004.5
-
Adsorption characteristics of bovine serum albumin and its peptide fragments on a stainless steel surface
T Sakiyama, J Tomura, K Imamura, K Nakanishi
COLLOIDS AND SURFACES B-BIOINTERFACES 33 ( 2 ) 77 - 84 2004.1
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ランダムペプチドライブラリーから選別した新規アフィニティータグによるポリスチレン表面に吸着したグルタチオンＳトランスフェラーゼの活性保持の改善
J. Mol. Catal. B: Enzymatic 28 (4), pp. 207-214 2004
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ステンレス表面に対する牛血清アルブミンとそのペプチド断片の吸着特性
Colloids Surf. B: Biointerfaces vol. 33(2), pp. 77-84 2004
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システインシンセターゼおよび関連酵素を発現し，O-アセチルセリンを分泌能が異なる組み替え大腸菌を用いた非タンパク性アミノ酸の生産
J. Biosci. Bioeng. 97(5), pp. 322-328 2004
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ステンレス表面に対する低分子物質の吸着におけるカルボキシル基の寄与
Journal of Colloid and Interface Science 279(2), pp. 289-295 2004
-
Contribution of acidic amino residues to the adsorption of peptides onto a stainless steel surface
K Imamura, Y Kawasaki, T Awadzu, T Sakiyama, K Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 267 ( 2 ) 294 - 301 2003.11
-
The kinetics of removal of proteins adsorbed on a stainless steel surface by H2O2-electrolysis and factors affecting its performance
K Imamura, Watanabe, I, M Imada, T Sakiyama, K Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 265 ( 1 ) 49 - 55 2003.9
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放線菌由来新規アシラーゼによるカプサイシン誘導体の合成とその抗酸化性の評価
KOREISHI MAYUKO, CHO TOKUMIN, KAWANAMI KAZUKO, IMAMURA YUKIKATSU, SAKIYAMA TAKAAKI, MATSUMURA YASUO, NAKANISHI KAZUHIRO
日本食品工学会年次大会講演要旨集 4th 117 2003.7
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食品製造プロセスにおける新規洗浄方法の原理とその特性 (特集 食品工場の洗浄・除菌技術)
今村 維克, 崎山 高明, 中西 一弘
ジャパンフ-ドサイエンス 42 ( 6 ) 27 - 33 2003.6
-
Effects of types of sugar on the stabilization of protein in the dried state
K Imamura, T Ogawa, T Sakiyama, K Nakanishi
JOURNAL OF PHARMACEUTICAL SCIENCES 92 ( 2 ) 266 - 274 2003.2
-
Effects of types of sugar on the stabilization of protein in the dried state
K Imamura, T Ogawa, T Sakiyama, K Nakanishi
JOURNAL OF PHARMACEUTICAL SCIENCES 92 ( 2 ) 266 - 274 2003.2
-
Ionization characteristics of polyelectrolyte complex gels: analysis based on their swelling behaviors
T. Sakiyama, T. Tsutsui, E. Masuda, K. Imamura, K. Nakanishi
Macromolecules 36 ( 13 ) 5039 - 5042 2003
-
ペプチドのステンレス表面への吸着に対する酸性アミノ酸残基の寄与
Journal of Colloid and Interface Science 276 ( 2 ) 294 - 301 2003
-
ステンレス表面に吸着したタンパク質のH2O2－電気分解による脱離の速度論と処理効果に及ぼす諸因子の影響
Journal of Colloid and Interface Science 265 ( 1 ) 49 - 55 2003
-
酵素法による脂質付加食品素材物質の大量合成法の開発と機能性の解明
中西 一弘, 今村 維克, 安達 修二
年報 2003 271 - 277 2003
-
In situ analysis of the removal behavior of protein adsorbed on a titanium surface by H2O2-electrolysis treatment
K Imamura, T Sakiyama, K Nakanishi
LANGMUIR 18 ( 21 ) 8033 - 8039 2002.10
-
In situ analysis of the removal behavior of protein adsorbed on a titanium surface by H2O2-electrolysis treatment
K Imamura, T Sakiyama, K Nakanishi
LANGMUIR 18 ( 21 ) 8033 - 8039 2002.10
-
Water sorption and glass transition behaviors of freeze-dried sucrose-dextran mixtures
K Imamura, A Fukushima, K Sakaura, T Sugita, T Sakiyama, K Nakanishi
JOURNAL OF PHARMACEUTICAL SCIENCES 91 ( 10 ) 2175 - 2181 2002.10
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A Novel Cleaning Method Using Electrolysis of Hydrogen Peroxide
IMAMURA Koreyoshi, NAKANISHI Kazuhiro
77 ( 899 ) 800 - 805 2002.9
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粉末系におけるゼインの二次構造に及ぼす水分活性と脂質添加の影響
MIZUTANI YUKIKO, MATSUMURA YASUO, IMAMURA TADAKATSU, NAKANISHI KAZUHIRO, MORI TOMOHIKO
日本食品科学工学会大会講演集 49th 152 2002.8
-
Removal of proteinaceous soils using hydroxyl radicals generated by the electrolysis of hydrogen peroxide
K Imamura, Y Tada, H Tanaka, T Sakiyama, K Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 250 ( 2 ) 409 - 414 2002.6
-
Removal of proteinaceous soils using hydroxyl radicals generated by the electrolysis of hydrogen peroxide
K Imamura, Y Tada, H Tanaka, T Sakiyama, K Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 250 ( 2 ) 409 - 414 2002.6
-
食品製造プロセスで発生する汚れの新規洗浄方法 (特集:食品製造装置の洗浄殺菌)
中西 一弘, 崎山 高明, 今村 維克
食品工業 45 ( 2 ) 18 - 24 2002.1
-
Adsorption behavior of methylene blue and its congeners on a stainless steel surface
K Imamura, E Ikeda, T Nagayasu, T Sakiyama, K Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 245 ( 1 ) 50 - 57 2002.1
-
Adsorption behavior of methylene blue and its congeners on a stainless steel surface
K Imamura, E Ikeda, T Nagayasu, T Sakiyama, K Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 245 ( 1 ) 50 - 57 2002.1
-
Influences of the Suspension Method of Thermolysin in Organic Solvents on Enzyme Activity
Keizo Tanaka, Koreyoshi Imamura, Takaharu SAkiyama, Kazuhiro Nakanishi
Japan Journal of Food Engineering 3 ( 1 ) 15 - 23 2002
-
Water sorption and glass transition behaviors of freeze-dried sucrose-dextran mixtures
Koreyoshi Imamura, Atsushi Fukushima, Keisuke Sakaura, Takuo Sugita, Takaharu Sakiyama, Kazuhiro Nakanishi
Journal of Pharmaceutical Sciences 91 ( 10 ) 2175 - 2181 2002
-
S219 Prospects for micro-bioreactor systems with respect to reactor and biocatalyst designs
Nakanishi Kazuhiro, Sakiyama Takaharu, Imamura Koreyoshi
14 21 - 21 2002
-
308 Analysis and control of adsorption behavior of enzyme onto a solid surface
Ohnishi Kousuke, Imamura Koreyoshi, Sakiyama Takaharu, Nakanishi Kazuhiro
14 28 - 28 2002
-
306 Water sorption and glass transition of freeze-dried disaccharidepolymer mixtures
Fukushima Atsushi, Sakaura Keisuke, Sugita Takuo, Imamura Koreyoshi, Sakiyama Takaharu, Nakanishi kazuhiro
14 28 - 28 2002
-
310 Removal of protein adsorbed on a metal surface by H_2O_<2^->electrolysis treatment
Imamura Koreyoshi, Watanabe Ippei, Sakiyama Takaharu, Nakanishi Kazuhiro
14 29 - 29 2002
-
309 Basic study on the control of enzyme binging onto polystyrene surfaces
Ueno Shunsuke, Sakiyama Takaharu, Imamura Koreyoshi, Nakanishi Kazuhiro
14 29 - 29 2002
-
549 Various substrate specificities of a novel acylase from Streptomyces sp.
Koreishi Mayuko, Zhang Demin, Takayanagi Eiko, Hata Kazuki, Imamura Koreyoshi, Sakiyama Takaharu, Nakanishi Kazuhiro
14 85 - 85 2002
-
Evaluation of hydration states of protein in freeze-dried amorphous sugar matrix
K Imamura, M Iwai, T Ogawa, T Sakiyama, K Nakanishi
JOURNAL OF PHARMACEUTICAL SCIENCES 90 ( 12 ) 1955 - 1963 2001.12
-
Cleaning of a stainless steel surface fouled with protein using a UV-H2O2 technique
K Imamura, Y Tada, H Tanaka, T Sakiyama, A Tanaka, Y Yamada, K Nakanishi
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 34 ( 7 ) 869 - 877 2001.7
-
Increase in the stability of serine acetyltransferase from Escherichia coli against cold inactivation and proteolysis by forming a bienzyme complex
K Mino, K Imamura, T Sakiyama, N Eisaki, A Matsuyama, K Nakanishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 65 ( 4 ) 865 - 874 2001.4
-
On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon
K Nakanishi, T Sakiyama, K Imamura
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 91 ( 3 ) 233 - 244 2001.3
-
UV/H2O2処理を利用したステンレス表面に付着したタンパク質の洗浄
34 ( 7 ) 869 - 877 2001
-
凍結乾燥によって得られた糖類アモリファスマトリクスにおけるタンパク質の水和状態の評価
Journal of Pharmacentical Sciences 91(12), 1955-1964 2001
-
Wada Eiko, Masuda Haruki, Imamura Koreyoshi, Sakiyama Takaharu, Adachi Shuji, Matsuno Ryuichi, Nakanishi Kazuhiro
13 158 - 158 2001
-
Purification of capsaicin-hydrolyzing enzyme from Streptomyces sp.
Koreishi Mayuko, Zhang Demin, Imamura Koreyoshi, Sakiyama Takaharu, Adachi Syuji, Matsuno Ryuichi, Nakanishi Kazuhiro
13 159 - 159 2001
-
pH-Responsive swelling of chitosan/chondroitin sulfate complex gels and its application.
Sakiyama Takaharu, Ikuta Yuji, Imamura Koreyoshi, Nakanishi Kazuhiro
13 282 - 282 2001
-
Cleaning of a stainless steel surface fouled with protein by H_2O_2-electrolysis technique
Imamura Koreyoshi, Tada Yoichiro, Tanaka Hirofumi, Sakiyama Takaharu, Nakanishi Kazuhiro, Tanaka Atsumi, Yamada Yosuke
13 282 - 282 2001
-
Cultivation characteristics of rerecombinant Aspergillus oryzae for production of enzyme
Shimamura Hiroko, Morita Masakazu, Yonashiro Ryota, Imammura Koreyoshi, Sakiyama Takaharu, Ninetoki Toshitaka, Nakanishi Kazuhiro
13 199 - 199 2001
-
バイオ・食品製造プロセスにおける汚れの付着と洗浄 (特集 最近の生物工学の展望)
中西 一弘, 崎山 高明, 今村 維克
ケミカルエンジニヤリング 45 ( 11 ) 854 - 861 2000.11
-
Adsorption behavior of amino acids on a stainless steel surface
K Imamura, T Mimura, M Okamoto, T Sakiyama, K Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 229 ( 1 ) 237 - 246 2000.9
-
Characteristics of serine acetyltransferase from Escherichia coli deleting different lengths of amino acid residues from the C-terminus
K Mino, K Hiraoka, K Imamura, T Sakiyama, N Eisaki, A Matsuyama, K Nakanishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 64 ( 9 ) 1874 - 1880 2000.9
-
Adsorption behavior of amino acids on a stainless steel surface
K Imamura, T Mimura, M Okamoto, T Sakiyama, K Nakanishi
JOURNAL OF COLLOID AND INTERFACE SCIENCE 229 ( 1 ) 237 - 246 2000.9
-
Water sorption and glass transition of amorphous sugars containing BSA
K Imamura, T Suzuki, T Tatsumichi, S Kirii, M Okazaki
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 33 ( 4 ) 657 - 660 2000.8
-
Kinetics and equilibrium for thermolysin-catalyzed syntheses of dipeptide precursors in aqueous/organic biphasic systems
M Miyanaga, M Ohmori, K Imamura, T Sakiyama, K Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 90 ( 1 ) 43 - 51 2000.7
-
Kinetic analysis for synthesis of a dipeptide precursor using an immobilized enzyme in water-immiscible organic solvents
M Miyanaga, K Imamura, T Sakiyama, K Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 90 ( 1 ) 112 - 114 2000.7
-
Development of novel advanced oxidation system using combined UV/H2O2 technique and kinetic analysis for decomposition of dye solutions
K Imamura, A Hiramatsu, M Imada, T Sakiyama, A Tanaka, Y Yamada, K Nakanishi
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 33 ( 2 ) 253 - 261 2000.4
-
膜面液体培養法を用いた海洋植物プランクトンOscillatoria属の培養と特性解明
中西 一弘, 崎山 高明, 今村 維克
財団法人ソルト・サイエンス研究財団助成研究報告集 1998 ( 1 ) 279 - 286 2000.3
-
BSAを含有した糖アモルファスの水分収着およびガス転移
Journal of Chemical Engineering of Japan 33 ( 4 ) 657 - 660 2000
-
紫外線/H2O2処理を利用した新規促進酸化システムの開発と色素水溶液の分解過程の速度論の解析
33 ( 2 ) 253 - 261 2000
-
Mino Koshiki, Imamura Koreyoshi, Sakiyama Takaharu, Eisaki Naoki, Matsuyama Asahi, Nakanishi Kazuhiro, RITE
12 225 - 225 2000
-
Synthesis of capsaicin analogues by aminoacylase.
Koreishi Mayuko, Kamakura Kaoru, Imamura Koreyoshi, Sakiyama Takaharu, Adachi Syuji, Matsuno Ryuichi, Nakanishi Kazuhiro
12 218 - 218 2000
-
Enzymatic synthesis of N-acylamino acids in cosolvents.
Wada Eiko, Handa Masato, Imamura Koreyoshi, Sakiyama Takaharu, Adachi Shuji, Matsuno Ryuichi, Nakanishi Kazuhiro
12 219 - 219 2000
-
Synthesis of nonproteinous amino acid by cysteine synthetase with acetyl-CoA regeneration system.
Ohno Katsuhiro, Mino Koshiki, Imamura Koreyoshi, Sakiyama Takaharu, Eisaki Naoki, Matsuyama Asahi, Nakanishi Kazuhiro
12 218 - 218 2000
-
Maintenance of protein structure in dry state by amorphous sugar
Imamura Koreyoshi, Ogawa Tomohiro, Sakiyama Takaharu, Nakanishi Kazuhiro
12 280 - 280 2000
-
Analysis for partition equilibrium of amino acid derivatives in aqueous/organic biphasic systems
M Miyanaga, K Imamura, K Tanaka, T Sakiyama, K Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 88 ( 6 ) 651 - 658 1999.12
-
Adsorption characteristics of tryptic fragments of bovine beta-lactoglobulin on a stainless steel surface
T Sakiyama, K Tanino, M Urakawa, K Imamura, T Takahashi, T Nagai, K Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 88 ( 5 ) 536 - 541 1999.11
-
Stability of immobilized thermolysin in organic solvents
M Miyanaga, M Ohmori, K Imamura, T Sakiyama, K Nakanishi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 87 ( 4 ) 463 - 472 1999.4
-
食品製造・バイオプロセスにおける洗浄とタンパク性汚れの付着(<特集>殺菌・洗浄のバイオテクノロジー)
中西 一弘, 今村 維克, 崎山 高明
生物工学会誌 : seibutsu-kogaku kaishi 77 ( 6 ) 241 - 245 1999
-
Characteristics and kinetics of cysteine synthetase from Escherichia coli.
Mino Koshiki, Imamura Koreyoshi, Sakiyama Takaharu, Eisaki Naoki, Matsuyama Asahi, Nakanishi Kazuhiro, RITE
11 170 - 170 1999
-
Adsorption behaviors of protein, peptide, and amino acid onto stainless steel surface
Imamura Koreyoshi, Sato Masako, Tomura Junji, Okamoto Makoto, Sakiyama Takaharu, Nakanishi Kazuhiro, Takahashi Tokio, Nagai Tadashi
11 333 - 333 1999
-
Production of pigment from Monascus pilosus k using membrane surface liquid culture
Tsushima Youko, Mino Akira, Imamura Koreyoshi, Sakiyama Takaharu, Nakanishi Kazuhiro, Suga Sadaharu, Danou Yasuhumi
11 220 - 220 1999
-
Purification and characterization of serine acetyltransferases truncated at C-terminal regions.
Hiraoka Kenji, Mino Koshiki, Imamura Koreyoshi, Sakiyama Takaharu, Eisaki Naoki, Matsuyama Asahi, Nakanishi Kazuhiro, RITE
11 167 - 167 1999
-
Ohno Katsuhiro, Mino Koshiki, Yamanoue Tsuyoshi, Imamura Koreyoshi, Sakiyama Takaharu, Eisaki Naoki, Matsuyama Asahi, Nakanishi Kazuhiro, RITE
11 184 - 184 1999
-
Shimamura Hiroko, Ishida Natsuko, Sugita Takuo, Imamura Koreyoshi, Sakiyama Takaharu, Nakanishi Kazuhiro, Ozeki Kenji
11 220 - 220 1999
-
Sugita Takuo, Segawa Takayuki, Shimamura Hiroko, Imamura Koreyoshi, Sakiyama Takaharu, Nakanishi Kazuhiro
11 219 - 219 1999
-
Influence of protein on phase transition of amorphous sugar
K Imamura, T Suzuki, S Kirii, T Tatsumichi, M Okazaki
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 31 ( 3 ) 325 - 329 1998.6
-
Fouling and cleaning of stainless steel surface: Adsorption and desorption behavior of bovine serum albumin and gelatin
T Sakiyama, T Toyomasu, A Nagata, K Imamura, K Nakanishi, T Takahashi, T Nagai
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 31 ( 2 ) 208 - 213 1998.4
-
Performance of protease as a cleaning agent for stainless steel surfaces fouled with protein
T Sakiyama, T Toyomasu, A Nagata, K Imamura, T Takahashi, T Nagai, K Nakanishi
JOURNAL OF FERMENTATION AND BIOENGINEERING 85 ( 3 ) 297 - 301 1998
-
Development of a cylindrical apparatus for membrane-surface liquid culture and production of kojic acid using Aspergillus oryzae NRRL484
Y Wakisaka, T Segawa, K Imamura, T Sakiyama, K Nakanishi
JOURNAL OF FERMENTATION AND BIOENGINEERING 85 ( 5 ) 488 - 494 1998
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Influence of protein on phase transition of amorphous sugar
A. Koreyoshiimamur, Tetsuo Suzuki, Shinya Kirii, Takayuki Tatsumichi, Morio Okazaki
Journal of Chemical Engineering of Japan 31 ( 3 ) 325 - 329 1998
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新規に開発したカビの膜面液体培養法を用いた食品加工用酵素の生産に関する基礎研究
中西 一弘, 崎山 高明, 今村 維克
食に関する助成研究調査報告書 ( 11 ) 51 - 58 1998
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Adsorption of amino acids and poptides onto the surface of stainless steel particles.
Mimura Tomoya, Tanino Kei, Imamura Koreyoshi, Sakiyama Takaharu, Nakanishi Kazuhiro
9 190 - 190 1997
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Cultivation of mold by membrane-surface liquid culture and its scale-up
Segawa Takayuki, Imamura Koreyoshi, Sakiyama Takaharu, Nakanishi Kazuhiro
9 96 - 96 1997
Presentations
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Spray-drying-Based Preparation and Characterization of Particulate and Nano-Fibrous Forms of Amorphous Solid Dispersion
Sato Haruna, Honda Nao, Hasegawa Takato, Araga Chika, Imanaka Hiroyuki, Ishida Naoyuki, Imamura Koreyoshi
Society of Chemical Engineering Japan 55th Autumn Meeting 2024.9.13
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Temperature scanning Fourier transform infrared spectroscopy of conformational changes of a temperature-responsive polymer in binary water/alcohol systems
Takezawa Ayumu, Ishida Naoyuki, Imanaka Hiroyuki, Imamura Koreyoshi
Society of Chemical Engineering Japan 55th Autumn Meeting 2024.9.12
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Preparation and Characterization of Fibrous Forms of Polysaccharides
Imamura Koreyoshi, Fukushima Kaito, Sato Haruna, Araga Chika, Imanaka Hiroyuki, Ishida Naoyuki
Society of Chemical Engineering Japan 55th Autumn Meeting 2024.9.12
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Stability of Proteinaceous Ligands in Storage: Evaluation of Interaction Detection Sensitivity After Immobilization
Watanabe Kota, Imamura Koreyoshi, Imanaka Hiroyuki
Society of Chemical Engineering Japan 55th Autumn Meeting 2024.9.11
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CutA1循環置換体を足場としたキメラ分子設計と機能評価
佐々木 統也, 今村 維克, 今中 洋行
第76回 日本生物工学回 2024.9.10
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Comparison among encapsulation characteristics of amorphous solid dispersion containing different molecular structures of flavors
Takato Hasegawa, Nao Honda, Haruna Sato, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura
25th Annual meeting of Japan Society of Food Engineering 2024.8.20
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Formation Conditions of Nano-Fibers of Drug Carrier Forming Materials by Spray-Drying with a Two-Fluidic Nozzle
N. Honda, C. Araga, T. Hasegawa, H. Sato, H. Imanaka, K. Imamura
39th Annual Meeting of the Academv of Pharmaceutical Science and Technology, Japan (APSTS) 2024.5.24
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Physicochemical Characteristics of Sole-Amorphous-Sugar-Based Solid Dispersion and Avoidance of Crystallization of Hydrophobic Drug
K. Imamura, R. Yamamoto, H. Imanaka, N. Ishida
The 39th Annual Meeting of the Academv of Pharmaceutical Science and Technology, Japan (APSTS) 2024.5.24
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タンパク質性足場 CutA1 循環置換体の設計と特性評価
佐々木統也,今村維克,今中洋行
日本農芸化学会中四国支部第 67 回講演会(例会) 2024.1.27
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CutA1 を利用したタンパク質性分子認識素子の設計と静電的相互作用モデル評価
光成麻弥, 渡邊滉大, 今村維克, 今中洋行
日本農芸化学会中四国支部第 67 回講演会(例会) 2024.1.27
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ペプチドリガンドの配置が生体分子間相互作用に及ぼす影響
渡邊滉大,光成麻弥,今村維克,今中洋行
日本農芸化学会 2023 年度中四国・西日本支部合同大会 2023.9.22
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香気成分のアモルファス固体分散における香料と高分子キャリアの相性の解析・評価
佐藤 春奈, 新田 有菜, 今中 洋行, 石田 尚之, 今村 維克
化学工学会第54回秋季大会 2023.9.12
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糖のみをキャリアとするアモルファス固体分散体における難水溶性薬剤の結晶化抑制
今村 維克, 山本 里奈, 今中 洋行, 石田 尚之
化学工学会第54回秋季大会 2023.9.11
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ナノバイオ界面におけるCutA1を足場としたタンパク質性高次構造体の設計
川上 達磨・ 今村 維克・ 今中 洋行
化学工学会第54回秋季大会 2023.9.11
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金属表面上に構築した高分子鎖層へのタンパク質の多層的捕捉とpH応答的放出挙動
西川 葵, 武澤 歩, 今中 洋行, 石田 尚之, 今村 維克
化学工学会第54回秋季大会 2023.9.11
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タンパク質性分子認識素子の大腸菌表層提示系構築の検討
河合 頼央, 今村 維克, 今中 洋行
第75回 日本生物工学会大会 2023.9.5
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金属表面の高分子コーティングによるタンパク質の三次元固定化
田坂 隆貴, 西川 葵, 今中 洋行, 石田 尚之, 今村 維克
化学工学会岡山大会2022 2022.12.19
Industrial property rights
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人工ナノファイバーを含む経口用組成物、及び人工ナノファイバーの製造方法（追加事項）
今村 維克, 今中 洋行, 佐藤 春奈
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人工ナノファイバーを含む経口用組成物、及び人工ナノファイバーの製造方法
今村 維克, 今中 洋行, 佐藤 春奈
Awards
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study award
2019.8 Japanese Society of Food Engineering Engineering study on the technique for enbedding and stabilizing biomolecules including food proteins
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encouragement award
2007.8 Japanese Society of Food Engineering Developmemt and innovation of novel cleaning technique based on electrolysis of hydrogen peroxide
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encouragement award
2002 Society of Chemical Engineering of Japan Study on advanced cleaning technique utilizing hydroxyl radical
Research Projects
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Development of an evaluation system for the stability of particle dispersions in non-aqueous liquids based on a new perspective
Grant number：21H01639 2021.04 - 2024.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Naoyuki Ishida
Grant amount：\16120000 （ Direct expense: \12400000 、 Indirect expense：\3720000 ）
The interaction forces between solid surfaces in organic solvents were measured using atomic force microscopy (AFM). The correlation between the affinity of solvent molecules to surfaces was evaluated using pulsed nuclear magnetic resonance (NMR) to investigate their influence on particle dispersion stability. It was confirmed that a high affinity between the surface and solvent molecules leads to repulsion between the surfaces due to the formation of a solvation layer. Conversely, low affinity to the solvent molecules results in attraction between the surfaces due to the absence of a solvation layer. Additionally, it was found that the formation of this solvation layer on surfaces coated with linear hydrocarbons is significantly influenced by factors such as chain length and molecular shape, in addition to the affinity between the surface and solvent molecules.
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Physico-chemical stabilization of surfactant-free solid dispersion and improvement of aqueous dissolution of water-insoluble drugs
Grant number：19H02499 2019.04 - 2023.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
今村 維克
Grant amount：\16120000 （ Direct expense: \12400000 、 Indirect expense：\3720000 ）
難水溶性薬剤の生体内における溶解性を改善する手法として，水可溶性のキャリアマトリクスに非晶質化した薬剤を分散する技術がある．申請者は，本来は“水と油”の関係にある糖と疎水性薬剤を，両親媒性物質を一切用いることなく，分子レベルで均一に混合できる原理的に新規な固体分散技術（Sole Amorphous Sugar based Amorphous Solid Dispersioin, SAS-SD, “surfactant-free固体分散”から呼称変更）を開発した．このSAS-SD試料において糖は水中で瞬時に溶解するため，疎水性薬剤の対水溶解性を既存の固体分散技術と同等以上に改善することができる．このSAS-SD技術は難水溶性成分の水溶性を高度に改善するが, ガラス転移温度 (Tg) が低く保存安定性に乏しいことが分かっていた．また他の固体分散技術と同様に, 難水溶性成分の溶解時間にも改善の余地が大きかった．そこでいくつかのモデル薬剤と糖を用いてSAS-SDを作成し，そのSAS-SD試料を難水溶性成分の融点以上の温度で熱処理（アニーリング）すると難水溶性成分の対水溶解性, Tgともに改善することが分かった．この難水溶性物質の溶解挙動の変化機構について基礎的な知見を得るためDSC等により固体分散試料の熱特性, 相互作用状態を評価した．その結果，アニーリングすることで糖分子間の相互作用密度が緩和され, 糖分子がより安定な構造をとっているためであることが示唆された．さらに，同じ骨格構造は持つ複数の誘導体（ibuprofen等）をモデル薬剤としてSAS-SD試料を作成し，アニーリング条件と薬剤の対水溶解性，およびモデル薬剤の物理化学的特性の関係について検討した結果，SAS-SDおよびそのアニーリングによる薬剤の対水溶解性の改善度は薬剤の融点を用いて相関できることを見出した．さらにSAS-SDの調製手法・条件の検討を行った．具体的にはSAS-SD試料を真空foam乾燥と噴霧乾燥の二種類の方法で調製し，その物性を比較・検討した．
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Induction of Foaming under Low Vacuum by Needle Stimulation of Solution for Controlling Drying Process in Vacuum Foam Drying
Grant number：15K14206 2015.04 - 2017.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
Imamura Koreyoshi
Grant amount：\3770000 （ Direct expense: \2900000 、 Indirect expense：\870000 ）
“Vacuum foam drying” is a promising drying technique that allows quick and effective desolvation without heating. However, “foaming” occurs randomly even under a sufficiently foamable condition and the timing of the foaming during the drying is hard to be controlled; The heterogeneous foaming results in different drying histories and consequently different characteristics of the dried products. On the other hand, we eventually found that, that, when the vacuum drying was aborted at a certain extent of drying and stimulated by sticking the sample solution with a steel needle, the resurgence of the vacuum drying immediately bring about the foaming at 100% probability. In order to establish the technique to uniform the product qualities obtained by “vacuum foam drying,” the impact of the needle stimulation on the solution foaming was herein investigated under different conditions. The mechanism by which the needle stimulation induces the inevitable foaming was also discussed.
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Heterogeneity of the state and functionality of water molecules sorbed in an amorphous sugar matrix
Grant number：23560908 2011 - 2013
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
IMAMURA Koreyoshi
Grant amount：\5070000 （ Direct expense: \3900000 、 Indirect expense：\1170000 ）
Amorphous materials, comprised of sugar molecules, exhibit high hygroscopicity and the sorbed water exerts a major influence on physical stabilities of the matrix. To date, the water sorption behavior of amorphous sugars has been extensively investigated. However, most studies of the behavior of water, when sorbed to an amorphous sugar matrix, have implicitly assumed that all of the sorbed water molecules are in a single state: It is naturally expected that the random allocation and configuration of sugar molecules would result in heterogeneity of states for the sorbed water. Recently, we demonstrated the heterogeneity of state and functionality of water sorbed in amorphous sugar matrices by combining a Fourier transform IR spectroscopy and Fourier self-deconvolution technique; The sorbed water molecules were classified into five states, and the three of them mainly served to lower the glass transition temperature of an amorphous sugar matrix while the other two are independent.
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Development of Site-Directed Immobilization Method to ConstructHigh-Efficient Bioreactors
Grant number：22560771 2010 - 2012
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
NAKANISHI Kazuhiro, IMAMURA Koreyoshi, IMANAKA Hiroyuki
Grant amount：\4550000 （ Direct expense: \3500000 、 Indirect expense：\1050000 ）
In this study, a method to immobilize enzymes/proteins on the solid surface without suffering from denaturation that might occur upon coming in contact with the solid surface was investigated. Conjugating peptide tags that show a high affinity to silica and polystyrene surfaces, toenzymes enabled a strong, nearly irreversible immobilization without appreciable loss of activity.
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Extraordinarily High Stabilizing Effect of Amorphous Sugar Matrix at Temperatures below Zero-Mobility Temperature
Grant number：20560702 2008 - 2010
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
IMAMURA Koreyoshi
Grant amount：\4420000 （ Direct expense: \3400000 、 Indirect expense：\1020000 ）
An amorphous matrix, comprised of sugar molecules, is known to stabilize labile substances such as proteins against chemical and physical degradations. On the other hand, this study revealed that a critical temperature, T*, exists, below which the disruption of sugar-sugar hydrogen bonds due to increasing temperature was substantially decreased relative to that at temperatures above the T*. In order to determine whether T* is the boundary temperature for achieving minimum molecular mobility, the relationship between T* with the mobility of sugar molecules in an amorphous matrix was investigated based on the structural relaxation behavior of amorphous sugar matrices at different temperatures. Furthermore, the T* values and the storage-stabilities of a protein for several types of sugars were examined and compared.
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親水性ポリスチレン高親和性ペプチドを用いた革新的蛋白質相互作用解析システムの創製
Grant number：19656221 2007 - 2008
日本学術振興会 科学研究費助成事業 萌芽研究
中西 一弘, 今村 維克, 今中 洋行
Grant amount：\3300000 （ Direct expense: \3300000 ）
本年度は、親水性ポリスチレン親和性ペプチドを用いた、1)コアストレプトアビジン(Core-SA)-DNA相互作用解析と2)相互作用に関与する機能性ペプチド部位を連結したクッションタンパク質を用いた相互作用解析を計画したが、両課題共に、初期の予定を達成できた。1)の課題においては、4量体のCore-SAに連結する親和性ペプチド(PSペプチド)の個数を変えて、その固定化特性とオリゴDNAとの相互作用をELISA法で検出した。結果として、4つのPSペプチドを連結したCore-SAは共存する高濃度牛血清アルブミンとの競合に影響されることなく、最も強固に固定化できることを示した。さらに、ビオチン標識オリゴDNAを連結したペプチドタグ連結コアストレプトアビジンは、市販のSA-coated plateよりも高い検出感度を示すことがわかった。一方、2)の課題に関しては,システイン合成酵素を構成するO-acetylserine sulfhydryrase-A(OASS-A)とそのC末端で相互作用することが知られているserine acetyltransferase(SAT)との間の相互作用に着目した。SATのC末端20残基からなるオリゴペプチドを、そのN末端にPSペプチドを連結したクッションタンパク質(RNaseHII)のC末端に連結し、オリゴペプチドのC末端アミノ酸を変化させてOASS-Aとの相互作用をELISA法で検出した結果、C末端アミノ酸がイソロイシンの野生型ペプチドを提示させた場合にのみ、相互作用することがわかった。同様に、Strep-tagIIをクッションタンパク質に提示したが、そのN末端をクッションタンパク質に向けて結合した場合にのみstreo-tactinと強い相互作用を示した。これらの結果から、本研究で検討した、親水性PS特異的親和性ペプチを用いる相互作用解析方法は、極めて高い検出感度と特異性を有することが明らかにされた。
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糖類アモルファスマトリクスのゼロモビリティー温度と不安定物質の半永久的保存
Grant number：18760594 2006 - 2007
日本学術振興会 科学研究費助成事業 若手研究(B)
今村 維克
Grant amount：\3500000 （ Direct expense: \3500000 ）
糖によって形されたアモルファスマトリクスは,タンパク質などの不安定物質を包括すると,その劣化を抑制する働きがある.この糖類アモルファスマトリクスによる包括安定化作用は,マトリクスを構成する糖分子の状態と密接な関係があると考えられる.従って,室温以下の低温域においては,極めて高度な保存性が期待できる.本研究では,昇温過程における糖分子の赤外線吸収(IR)スペクトルを連続的に測定できる温度操作フーリエ変換赤外分光分析(TS-FTIR)により,-20℃からTg前後の温度域における糖分子間相互作用(水素結合)の形成状態を解析した.すなわち,糖水酸基の伸縮振動に起因するIR吸収帯のピーク波数を温度に対してプロットした.その結果,ピーク波数は温度上昇に伴い高波数側にシフトしていくが,Tgより数十℃低いある温度(T*)においてもピーク波数の増加勾配が顕著に小さくなることが分かった.これより,糖分子間水素結合が極めて安定に存在する温度域(<T*)が存在することが示唆された.また,その温度域の上限(T*)はsucroseで顕著に低く,糖の分子量とともに高くなる傾向が見られた.
一方,糖類アモルファスマトリクスの構造エンタルピーの緩和過程を示差走査熱量分析(DSC)により測定し,異なる温度における糖分子のモビリティーを評価・比較した.その結果,上述のT*以下の温度領域では,各糖試料の構造エンタルピーの緩和が認められなかった.さらに,酵素を用いて糖類アモルファスマトリクスで包括したときの失活速度の温度依存性を調べたところ,T*以下では酵素の失活がほとんど生じないことが分かった.以上のことから,T*は糖分子のモビリティーが極端に制限される温度領域の上限値を表しており,この温度以下では極めて高度な包括安定化作用が発現することが明らかとなった. -
Molecular mechanism for adsorption and adsorption of soil components formed on the surface of equipment wall during manufacturing and development of an efficient cleaning method
Grant number：16206076 2004 - 2007
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
NAKANISHI Kazuhiro, IMAMURA Koreyoshi, IMANAKA Hiroyuki
Grant amount：\46800000 （ Direct expense: \36000000 、 Indirect expense：\10800000 ）
In various manufacturing processes, cleaning of the equipment and piping systems used for manufacturing of various products is requisite for maintaining sanitary conditions and/or functions of the equipments. In particular, in the food- and bio-products manufacturing processes, a large amount of energy and detergents are usually consumed in addition to time, since soils or residues of the products strongly interact with the equipment surface. In this study, we studied adsorption mechanisms of various proteins/peptides and low-molecular weight substances as a model soil component on the surfaces of various metal oxides, plastics, and glasses. We clarified the molecular mechanisms for reversible/irreversible adsorptions, states and orientation of the molecules adsorbed, the effects of the pI values of the metal oxide surfaces/solution pHs on the adsorption characteristics, and conformational change of the adsorbed proteins, on the basis of adsorption/desorption experiments, IR spectrum analyses, and molecular calculations. Furthermore, we investigated the effects of various factors such as the kinds of the co-existing salts and their concentrations on the removal rates of the metal oxide surface fouled with proteins, using H2O2-electrolysis cleaning method that was developed by the author's group. Furthermore, we developed a method to immobilize proteins with controlled orientation and structure for various uses in chip-based biotechnology, which was derived in the course of investigation on the adsorption mechanism of proteins.
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高度なタンパク質安定化作用と物理的安定性を兼備した新規糖類アモルファス構造の創製
Grant number：15760558 2003 - 2004
日本学術振興会 科学研究費助成事業 若手研究(B)
今村 維克
Grant amount：\3500000 （ Direct expense: \3500000 ）
目的
高度なタンパク質安定化作用と物理的安定性を併せ持つ新規な糖類アモルファスマトリクスの構築を最終目標として,高度なタンパク質安定化作用を有する少糖と高い物理的安定性を有する多糖を組み合わせた少糖-多糖複合アモルファスの物理特性およびマトリクス内における分子間相互作用について検討した.
実験方法
スクロ-スおよびデキストランを様々な割合(0〜100%)で含む水溶液を調製した.一部の試料溶液には塩を0.1〜100mMになるように添加した.試料溶液を液体窒素で急速凍結して室温で1日凍結乾燥した.その後,さらにP_2O_5を入れた真空デシケータ内(37℃)で3日間絶乾処理することにより,少糖-多糖複合アモルファス試料を得た.少糖-多糖複合アモルファス内の分子間相互作用を解析するため,試料を一定相対湿度(RH 11,23,33%)下で平衡化後,含水率,ガラス転移温度(Tg)を測定した.試料の含水率は重量法およびKarl-Fischer法により測定し,ガラス転移温度は示差走査熱分析により測定した.
結果
スクロースーデキストラン複合アモルファス試料の水分収着特性を解析した結果,複合試料の平衡含水率はスクロースおよびデキストランそれぞれ単体のときの値から算出される理論値よりも低くなることが分かった.これはスクロースとデキストランが共存することでスクロース-デキストラン分子間に相互作用が形成し,それぞれ単体で存在するときよりも水和サイトが減少したことを意味している.一方,スクロース-デキストラン複合アモルファス試料のガラス転移温度に及ぼす塩の影響を調べた結果,リン酸塩の共存によるガラス転移温度の変化は認められなかったが,塩化ナトリウムや塩化アンモニウムはガラス転移温度を低下させることが分かった. -
Adhesion and Removal Mechanisms of Composite Soils Formed on the Equipment Surface During Food Processing
Grant number：13450319 2001 - 2003
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
NAKANISHI Kazuhiro, IMAMURA Koreyoshi, SAKIYAMA Takaharu
Grant amount：\15100000 （ Direct expense: \15100000 ）
In food manufacturing processes, proteinaceous soils derived from foods tend to strongly adhere to the サball surface of equipment/pipes and thus a lot of energy and detergents are usually consumed for cleaning of the equipment. Although it is strongly required to save energy and detergents by optimizing the cleaning conditions, few systematic approaches have been made. In this project, the intended to reveal scientifically the mechanism for adsorption of proteinaceous soils onto the metal surfaces and that for removal. As a model proteinaceous soil, we usually adopted β-lactoglobulin (β-Lg) and sometimes used a composite of the protein and tannic acid. Particles and plates of stainless steel were mostly used as a model surface. The findings obtained in this projects are summarized as follows.
1. Interaction peptide regions of β-Lg that directly interact with a stainless steel surface was analyzed by the two methods : 1) peptide fragments of β-Lg digested with protease were first in contact with the surface and peptides strongly interacting were identified and 2) β-Lg that had been adsorbed on the surface was subjected to digestion with protease and then the kinds and composition of the remaining peptides were analyzed. As a result, we found that peptide regions containing plural, acidic amino, acids are responsible for direct interaction with the surface at a pH region lower than neutral pH. With increasing the number of acidic amino acids of the peptide, affinity as well as the amount of adsorptiotn increased. Adsorption isotherms could be explained by taking into consideration the both reversible and irreversible adsorption modes.
2. We developed a novel cleaning method, in which negative electropotential (around -0.2〜-0.8V vs. Ag/AgCl) is added to the metal surface being in contact with dilute H_2O_2 solution to generate hydroxyl radicals and generated radials could quickly decompose proteinaceous soils adsorbed on the surface. The proteinaceous soil strongly adsorbed on a stainless steel surface there found to be removed completely within 2 min at room temperature while the removal rate by caustic cleaning was much slower. The various factors including coexisting salt concentration, potential, and current on the removal kinetics there analyzed in details.
3. Adsorption mechanism of the composite soil composed of β-Lg and tannic acid was analyzed under different conditions ~ using RAS-IR. The H_2O_2-electrolysis cleaning method was quite effective to remove the soil from the surface. Namely, tannic acid was removed effectively even by caustic cleaning while sufficient removal of β-Lg was possible only by the H_2O_2-electrolysis cleaning method at room temperature. -
高度なタンパク質安定化機能を有する糖類アモルファスマトリクスの創製
Grant number：12750664 2000 - 2001
日本学術振興会 科学研究費助成事業 奨励研究(A)
今村 維克
Grant amount：\2200000 （ Direct expense: \2200000 ）
目的 不安定なタンパク質を安定化する手法の一つとして、タンパク質を糖などの安定化剤とともに凍結乾燥することでタンパク質を糖のアモルファスマトリクスに包埋し、保存中の変性、失活を低減する手法がある。このタンパク質安定化剤としてはスクロースなどの低分子量の糖は高い安定化作用を有するものの、吸湿や熱により容易にコラプスするという欠点がある.一方,デキストランなどの多糖類は安定化作用は極めて低いものの,物理的安定性に優れている。従って,高い安定化作用を有する少糖と高い物理的安定性を有する多糖を組み合わせることで,高度な安定化作用と物理的安定性を併せ持つ新規な糖類アモルファスマトリクスを構築出来る可能性がある.この新規な安定化剤である少糖-多糖複合アモルファスの物理的安定性(ガラス転移温度,Tg)およびタンパク質安定化作用について検討した。
方法 少糖としてsucrose, erythritol,多糖として分子量の異なる2種類のdextran(MW1500, 6000),タンパク質としてlactate dehydrogenase (LDH)を用いた.これらの少糖・多糖を様々な割合(0〜100%)で含む凍結乾燥試料を作成し,凍結乾燥直後のLDHの相対残存活性,一定相対湿度下で平衡化したときのTgを測定した.
結果 sucrose-dextran, erythritol-dextran試料のTgはいずれの相対湿度においてもdextran含有率が40%以上で顕著に上昇することが分かった。一方,凍結乾燥直後の残存酵素活性は,sucrose crythritol単体の場合はいずれも50%であったのに対し,sucrose-dextran試料では最大70%, erythrito1-dextran試料では最大95%となった.これらのことより少糖-多糖複合アモルファスマトリクスの極めて高い有効性が明らかとなった. -
固体表面に対する配向の精密制御されたタンパク質の創製
Grant number：12019251 2000
日本学術振興会 科学研究費助成事業 特定領域研究(A)
中西 一弘, 今村 維克, 崎山 高明
Grant amount：\2000000 （ Direct expense: \2000000 ）
タンパク質(酵素)の固体表面に対する付着は、分析、反応、生体材料設計など生物生体工学の多岐の分野においてみられれ現象であり、それぞれの応用を考える際には付着に対する親和性と配向の制御が重要な鍵となる。そこで、本研究では、タンパク質の固体表面に対する相互作用と直接付着に関与する部位の解明を行うと共に固体表面に対する親和性と配向の制御されたタンパク質の創製を最終目的としている。タンパク質として分子量が小さく、性質や立体構造が明らかにされているウシ膵臓ribonucleaseA(RNaseA)に着目して、固体表面に直接相互作用するタンパク質分子内ペプチド部位の解明と固体表面に対する親和性及び配向の制御された酵素の創製を行うために、変異RNaseAの調製法を確立すると共にその付着挙動を調べた。変異RNaseAとしては、ステンレス表面に対する付着部位と推定されている、^<47>His-Glu-Ser-Leu-Ala-Asp-Val^<57>中のAlaをAspに置換したAla55Asp変異RNaseAとGluとAspのそれぞれあるいは両方をAlaに置換した、Glu55Ala変異RNaseAとAsp55Ala変異RNaseA、及びPS Latexに対し強い付着を示す^<117>Pro-Val-His-Phe-Asp-Ala-Ser-Val^<124>中のPheをAlaに置換したPhe120Ala変異RNaseAを調製した。いずれの場合も変異RNaseA遺伝子をプラスミドpBXRに組み込んだ発現プラスミドを作成し、大腸菌(BL21)内で発現させた。培養大腸菌を回収後に超音波破砕を行い、封入体を回収した。回収した封入体を変性・再生後にイオン交換クロマトグラフィーとゲル濾過により精製した。精製酵素はSDS-PAGE上で均一であることを確認した、精製酵素の収率は10%前後であった。精製酵素のステンレス粒子及びPS Latexに対する付着特性を調べた。予備的検討の結果、予想した通りにAla55Asp変異RNaseAはステンレス粒子に対して野生型RNaseAよりも強い付着力を示したが、Phe120Ala変異RnaseAでは変化がみられなかった。
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固体表面に対する親和性と配向の精密制御されたタンパク質の創製
Grant number：11132250 1999
日本学術振興会 科学研究費助成事業 特定領域研究(A)
中西 一弘, 今村 維克
Grant amount：\2000000 （ Direct expense: \2000000 ）
本研究では、タンパク質と固体表面の直接の相互作用に着目して、固体表面に対する親和性と配向の制御されたタンパク質の創製を最終目的として、1)固体表面に直接相互作用するタンパク質分子内ペプチド部位を特定する方法の確立と、2)ペプチド部位の固体表面に対する親和性の強さとそのアミノ酸組成・長さ・二次構造等との関係を明らかにすることである。本年度はモデルタンパク質として、主として分子量が小さく三次元構造が明らかにされている、ウシ膵臓由来リボヌクレアーゼA(RNaseA)を、固体表面としてステンレス粒子とポリスチレンラテックス(Pslatex)を用いて、固体表面に直接相互作用する部位の同定と相互作用に関する知見を得ることを目的とした。RNaseAをカルボキシメチル化した後、トリプシン処理し、得られたペプチド混合液あるいは合成したペプチドを試験液に溶解し、吸着等温線を測定した。主な成果は次の3点に要約される。
1)RNaseAの各pHにおけるステンレスに対する吸着等温線はいずれもLangmuir型を示したが、高pH領域では、殆ど吸着がみられなかった。Pslatexの場合では吸着等温線はpHに殆ど依存せず、不可逆吸着を示した。
2)トリプシン処理によって得られたRNaseA由来ペプチドを用いて吸着実験を行ったところ、ステンレスに対しては酸性アミノ酸残基を含むペプチド10が、Pslatexに対しては、疎水性アミノ酸残基を多く含むペプチド9が顕著に吸着していた。3)ペプチド9-1の疎水性アミノ酸残基であるIleをGlyに、ペプチド9-3のPheをGlyに、10-1の酸性アミノ酸残基GluとAspをGlyに置換したペプチドを合成し、吸着特性の変化を調べたところ、いずれのペプチドも吸着力は激減した。これらの結果から、ステンレスに対する吸着には酸性アミノ酸残基が、Pslatexに対する吸着には、疎水性アミノ酸残基による疎水相互作用とベンゼン環同士のπ-π相互作用が深く関与していることが示唆された。 -
Clarification of Adhesion Mechanisms of Soils onto the Equipment Wall Surfaces and Its Desorption in Food Manufacturing Process
Grant number：10450287 1998 - 2000
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B).
NAKANISHI Kazuhiro, IMAMURA Koreyosi, SAKIYAMA Takaharu
Grant amount：\14100000 （ Direct expense: \14100000 ）
In this study, we focussed to the following three subjects, i.e., 1) clarification of adsorption characteristics of proteins, peptides, and amino acids, 2) identification of peptide regions in protein molecules that interact with solid surfaces, and 3) cleaning behaviors of the surfaces fouled with proteins using proteolytic enzymes and hydroxyl radicals generated by combined hydrogen peroxide and ultraviolet rays. Adsorption behaviors of β-lactoglobulin, bovine serum albumin, and gelatin onto the stainless steel surface were investigated under different conditions. With increasing the temperature, the adsorbed amount of β-lactoglobulin and bovine serum albumin were increased to form disulfide linkages, whereas that of gelatin decreased. Furthermore, adsorption behaviors of amino acids and peptides were examined in detail, which showed the importance of acidic amino acids in the adsorption behaviors. The adsorption behaviors of peptides obtained by digestion of β-lactoglobulin with trypsin were investigated. It was found that a peptide fragment (^<125>Thr-Pro-Glu-Val-Asp-Asp-Glu-Ala-Leu-Glu-Lys^<136>) showed an irreversible adsorption behavior and this region was thought to be one of the interaction sites in the protein. FT-IR analyses revealed that the acidic amino acids were dissociated on adsorption onto the stainless steel surface. Cleaning behaviors of the stainless steel particles fouled with proteins by enzyme cleaning (proteases) and hydroxyl radical cleaning were carried out under various conditions to compare their performances with caustic cleaning and their cleaning mechanisms were studied.
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Development of a Novel Cultivation Method for Molds and Its Characterization
Grant number：10555291 1998 - 2000
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B).
NAKANISHI Kazuhiro, NAGASAWA Tooru, IMAMURA Koreyosi, SAKIYAMA Takaharu
Grant amount：\13200000 （ Direct expense: \13200000 ）
In this, study we applied a newly-developed membrane-surface liquid culture (abbreviated as MSLC) to production of useful substances using molds. We not only intended to optimize the cultivation conditions but also clarify the reasons for the higher productivity obtained in MSLC in comparison with the results obtained in shaking flask cultures. We studied three fermentation systems, i.e., 1) kojic acid fermentation using Aspergillus oryzae, 2) red pigment production using Monascus pilosus, and 3) production of α-glucosidase using recombinant Aspergillus oryzae under various cultivation conditions, different in factors such as pH of the medium and C-and N-source concentrations. In MSLC, we first examined the effect of kinds of porous membranes on the cultivation behaviors. The most suitable membrane was found to be such a membrane that have a pore diameter of around 0.2μm and hydrophilic property. MSLC gave a higher productivity in the all three cultivation systems examined than those with shaking flask cultures. We examined the effect of oxygen supply in the shaking flask cultures to seek the reasons for the higher productivity in MSLC.The reasons for the higher productivity in MSLC were found not to be in the higher oxygen supply. It was suggested that differences in gene expression, the amount of relating enzyme produced in the cells, and permeability of the product through the cell membrane may affect the higher productivity in MSLC.
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糖-タンパク質間水素結合のタンパク質の安定化に対する寄与とその評価
Grant number：10750548 1998 - 1999
日本学術振興会 科学研究費助成事業 奨励研究(A)
今村 維克
Grant amount：\2000000 （ Direct expense: \2000000 ）
タンパク質水溶液に糖を添加し凍結乾燥すると,タンパク質は糖によって形成されたアモルファス構造に包埋され,その結果,タンパク質の安定性が顕著に向上する.この糖によるタンパク質安定化機構についてはこれまで多くの研究がなされているが,依然未解明な点が多く,実際の安定化操作の改善に繋がる知見は乏しい.そこで本研究では糖としてスクロース,マルトース,トレハロース,マルトトリオース,および平均分子量1500,6000のデキストラン,タンパク質として牛血清アルブミン(BSA)を様々な割合で含有する糖-タンパク質凍結乾燥資料を作成し,試料中におけるタンパク質の構造保持の度合いを比較した.タンパク質の構造保持度はフーリエ変換赤外分光分析計(FT-IR)を用いて二次構造を解析することにより評価し,各種糖アモルファスの物理的特性は示差走査熱量計(DSC)を用いて分析した.また,タンパク質安定化作用との密接な関係が注目されている糖-タンパク質間水素結合を水分収着特性から定量し,タンパク質の構造保持度との関係について検討を加えた.その結果,タンパク質の構造保持度をαへリックスの含有率を指標として評価した場合,構造保持の度合いはスクロース>マルトース>トレハロース>マルトトリオース>デキストラン1500>デキストラン6000の順となり,スクロースを用いた場合,糖添加量が1g/g-BSAのとき水溶液中の値とほぼ一致した.各種糖アモルファスのガラス転移温度(Tg)を測定した結果,Tgが低い糖ほどタンパク質の構造保持度が高いことが分かった.これはTgが低い糖アモルファスほど糖分子の構造柔軟性が高く,タンパク質との相互作用が形成され易いためと考えられる.また,糖-タンパク質間水素結合の形成度を評価した結果,糖-タンパク質間水素結合の形成度は構造保持の度合いと大略傾向は一致することが明らかになった.
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固体表面に対する親和性と配向の精密制御された抗体の創製
Grant number：10145237 1998
日本学術振興会 科学研究費助成事業 特定領域研究(A)
中西 一弘, 今村 維克
Grant amount：\2000000 （ Direct expense: \2000000 ）
平成10年度は、比較的分子量の小さいRNAseやβ-ラクトグロブリン(β-Lg)をモデルタンパク質として用いて、固体表面に直接接触しているぺプチド部位の特定と相互作用の強さに関して検討を試みた。直接付着に関与するぺプチド部位を特定する方法としては、タンパク質のプロテアーゼ消化により得られるぺプチド類の付着挙動から推定する間接的な方法を用いた。タンパク濃度一定条件下では、等電点近傍のpH5近傍で最大の吸着量を示した。吸着等温線(30℃)は見掛けLangmuir型を示した。酸性領域(pH3〜4)では、イオン強度が高いほど、吸着平衡定数は大きい結果となった。β-Lgをトリプシンで処理することにより14種類のペプチドを単離した。各ペプチドの組成と配列を一次構造とアミノ酸分析により同定した。ペプチド混合溶液あるいは各ペプチド単独の溶液を用いて種々の条件下(PH、温度、イオン強度)で吸着挙動を調べた。β-Lgが高い吸着を示した酸性pH領域では酸性アミノ酸を複数個含むペプチドがステンレス粒子に対する親和性が高いことが判明した。これは、ペプチド分子中の酸性アミノ酸残基がマイナスに誘起されて、ステンレス表面のプラスの電荷と多点結合するためであることが示唆された。同様にトリプシン処理によって得られたカルポキシメチル化RNaseA由来ペプチドを単離(10種類)し、そのステンレス粒子及びラテックスに対する吸着挙動をペプチド混合溶液を用いて検討した。いずれのペプチドもステンレスに対する吸着は、殆どみられなかった。一方、ラテックスに対する吸着は疎水性アミノ酸残基が多く含むペプチドにおいて強いことから、ラテックスに対する吸着には疎水性相互作用が寄与していることが示唆されたが、ペプチドとRNaseAの吸着挙動には、β-Lgのような明確な相関関係はみられなかった。
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バイオおよび食品製造プロセスにおける洗浄操作の高効率化によるエミッションの削減
Grant number：10141228 1998
日本学術振興会 科学研究費助成事業 特定領域研究(A)
中西 一弘, 今村 維克
Grant amount：\2000000 （ Direct expense: \2000000 ）
本研究の目的は,バイオプロセスにおいて膨大なエミッションを生み出す洗浄操作の合理化ある.平成10年度はOHラジカルの高い酸化力に着目し,(1)固体表面汚れの効率的除去と(2)洗浄排水の低減の可能性を検討した.
(1) 付着汚れの除去への応用は次のような原理に基づいている.H_2O_2水溶液を汚れの付着した固体表面に薄い液膜として流し,その上部から紫外線を照射すると,H_2O_2の紫外線分解によりOHラジカルが発生し,付着状態および脱着・遊離した汚れ物質を酸化分解する.さらに,光照射により汚れ物質の脱着が促進される.モデルは汚れ物質として脂肪酸を付着させたステンレス表面を用いて,付着汚れのラジカル酸化除去効果および紫外線による脱離促進効果を検証した.脂肪酸の付着・残留量はRAS-FT-IRにより定量した.その詰果,水流だけでは脂肪酸はほとんど脱離しないが,紫外線の照射により顕著に脂肪酸が脱離することが分かった.
(2) 洗浄排水処理へのラジカル酸化分解の応用については,モデル有機物質として色素を含む着色水溶液のUV-H_2O_2処理における脱色特性を明らかにした.いづれの色素についても脱色反応は一次反応に従い,見かけの一次反応速度定(K)のH_2O_2濃度依存性を求めたところ,H_2O_2濃度に最大値が存在した.これはH_2O_2濃度の増加伴い,OHラジカルの発生速度とともにUVの減衰が顕著になるためであると考えられる.そこで,紫外線照射下のH_2O_2-色素水溶液におけるラジカル反応(H_2O_2の紫外線分解反応,OHラジカルとH_2O_2との反広,OHラジカルと色素との反応)とH_2O_2および色素による紫外線の減衰を考慮したモデルを設定し,kの理論値を計算した.その結果,実験値と良好に一致し,本モデルにより有機物質のラジカル酸化速度に対する条件の最適化が可能である. -
Improvement of stability and reaction behavior of enzymes in organic solutions by immobilization onto nano-porous supports
Grant number：09555256 1997 - 1998
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
IMAMURA Koreyosi, UTAGAWA Takashi, NAKANISHI Kazuhiro
Grant amount：\1100000 （ Direct expense: \1100000 ）
In this study, we intended to establish theoretical bases, which are required to realize enzymatic syntheses in organic solvents. We studied the synthetic reaction of aspartame precursor, Z-L-Asp-L-PheOMe, from respective amino acid derivatives (Z-Asp and PheOMe) catalyzed by thermolysin. First, we investigated various factors affecting stability of the immobilized thermolysin and analyzed its mechanism. Autolysis was found to be a main cause for inactivation in water-immiscible organic solvents and the immobilized enzyme tended to be inactivated in organic solvents with lower solubility of water. In particular, the immobilized was stable in tert-amyl alcohol probably because that aqueous phase around the enzyme immobilized is thin and as a result autolysis tended to be repressed. The method to predict the overall partition coefficient of the substrates containing acid and amine components was proposed, by taking into consideration the effect of ion-pair complexes formed between the substrates.
The synthetic rate with the immobilized enzyme in an organic solvent, containing a small amount of water could be in principle estimated on the basis of the model for the biphasic reaction when the amount of enzyme loaded was not so much. Namely, in the synthetic reactions, the both components of the substrate are partitioned into the water phase inside the support, and then converted to the product by the action of the enzyme, and finally the product is transferred into the bulk organic phase. -
Adsorption & desorption of protein, peptide, & amino acids
Grant type：Competitive
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糖によるタンパク質の安定化
Grant type：Competitive
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タンパク質・ペプチド・アミノ酸の金属壁面への付着・脱離
Grant type：Competitive
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ラジカル酸化反応の排水処理,固体表面の洗浄への応用
Grant type：Competitive
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Application of radical oxidation to waste water treatment & cleaning of Solid Surfaces
Grant type：Competitive
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Stabilization of protein by sugars
Grant type：Competitive
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Adsorption & desorption of protein, peptide, & aminoocids
Grant type：Competitive
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Application of radical oxidation to waste water treatment & cleaning of Sclid Surfaces
Grant type：Competitive
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Stabilization of protein by sugar
Grant type：Competitive
Class subject in charge
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Seminar in Bioprocess Engineering （2024academic year） Year-round - その他
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Analysis and Application of Intermolecular Interactions （2024academic year） Late - その他
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Basic of Applied Chemistry and Biotechnology （2024academic year） 1st semester - 金1～2
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Chemical Engineering 1A （2024academic year） Third semester - 水1～2
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Chemical Engineering 1B （2024academic year） Fourth semester - 水1～2
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Chemical Engineering 1 （2024academic year） 3rd and 4th semester - 水1～2
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Seminar on Applied Chemistry 1 （2024academic year） Prophase - その他
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Seminar on Applied Chemistry 2 （2024academic year） Late - その他
-
Research Works for Master Thesis on Applied Chemistry （2024academic year） Year-round - その他
-
Seminar in Advanced Chemistry （2024academic year） Other - その他
-
Materials and Reaction Process Engineering （2024academic year） Late - 火1～2
-
Physical Chemistry 2 （2024academic year） 1st semester - 月3～4,木1～2
-
Physical Chemistry 2 （2024academic year） 1st semester - 月3～4,木1～2
-
Advanced Study （2024academic year） Other - その他
-
Biochemiacl Engineering （2024academic year） Late - その他
-
Biointerface Engineering （2024academic year） Late - その他
-
Seminar in Bioprocess Engineering （2023academic year） Year-round - その他
-
Seminar in Bioprocess Engineering （2023academic year） Year-round - その他
-
Design of Biocatalysts and Bioprocesses （2023academic year） Prophase - その他
-
Leading-edge Technology in Materials and Process Innovation 4 （2023academic year） Summer concentration - その他
-
Analysis and Application of Intermolecular Interactions （2023academic year） Late - その他
-
Basic of Applied Chemistry and Biotechnology （2023academic year） 1st semester - 金1～2
-
Chemical Engineering 1A （2023academic year） Third semester - 水1～2
-
Chemical Engineering 1B （2023academic year） Fourth semester - 水1～2
-
Chemical Engineering 1 （2023academic year） 3rd and 4th semester - 水1～2
-
Chemical Engineering I （2023academic year） Fourth semester - 水1～2
-
Internship in Applied Chemistry （2023academic year） Prophase - その他
-
Seminar on Applied Chemistry 1 （2023academic year） Prophase - その他
-
Seminar on Applied Chemistry 2 （2023academic year） Late - その他
-
Research Works for Master Thesis on Applied Chemistry （2023academic year） Year-round - その他
-
Seminar in Advanced Chemistry （2023academic year） Other - その他
-
Topics in Material Process 4 （2023academic year） Summer concentration - その他
-
Materials and Reaction Process Engineering （2023academic year） Late - 火1～2
-
Advanced Materials Chemistry 4 （2023academic year） Summer concentration - その他
-
Thermodynamic Description of Mixtures （2023academic year） 1st semester - 月4,木2
-
Thermodynamics III （2023academic year） 1st semester - 月3,木1
-
Physical Chemistry 2 （2023academic year） 1st semester - 月3～4,木1～2
-
Physical Chemistry 2 （2023academic year） 1st semester - 月3～4,木1～2
-
Advanced Study （2023academic year） Other - その他
-
Biochemiacl Engineering （2023academic year） Late - その他
-
Biointerface Engineering （2023academic year） Late - その他
-
Seminar in Bioprocess Engineering （2022academic year） Year-round - その他
-
Design of Biocatalysts and Bioprocesses （2022academic year） Prophase - その他
-
Basic of Applied Chemistry and Biotechnology （2022academic year） 1st semester - 金1～2
-
Chemical Engineering 1A （2022academic year） Third semester - 水1～2
-
Chemical Engineering 1B （2022academic year） Fourth semester - 水1～2
-
Chemical Engineering 2A （2022academic year） 1st semester - 火1～2
-
Chemical Engineering 2B （2022academic year） Second semester - 火1～2
-
Chemical Engineering (Ⅰ) （2022academic year） 3rd and 4th semester - 水1～2
-
Chemical Engineering 1 （2022academic year） 3rd and 4th semester - 水1～2
-
Chemical Engineering (Ⅱ) （2022academic year） 1st and 2nd semester - 火1～2
-
Chemical Engineering I （2022academic year） Fourth semester - 水1～2
-
Fundamental of Chemical Engineering （2022academic year） Third semester - 水1～2
-
Internship in Applied Chemistry （2022academic year） Prophase - その他
-
Seminar on Applied Chemistry 1 （2022academic year） Prophase - その他
-
Seminar on Applied Chemistry 2 （2022academic year） Late - その他
-
Basic Applied Chemistry （2022academic year） Late - 月3～4,火3～4
-
Research Works for Master Thesis on Applied Chemistry （2022academic year） Year-round - その他
-
Topics in Material Process 3 （2022academic year） Summer concentration - その他
-
Advanced Materials Chemistry 7 （2022academic year） Summer concentration - その他1～8
-
Thermodynamic Description of Mixtures （2022academic year） 1st semester - 月4,木2
-
Thermodynamics III （2022academic year） 1st semester - 月3,木1
-
Physical Chemistry and Exercises 2 （2022academic year） 1st semester - 月3～4,木1～2
-
Physical Chemistry 2 （2022academic year） 1st semester - 月3～4,木1～2
-
Physical Chemistry 2 （2022academic year） 1st semester - 月3～4,木1～2
-
Biochemiacl Engineering （2022academic year） Prophase - 木1,木2
-
Seminar in Bioprocess Engineering （2021academic year） Year-round - その他
-
Design of Biocatalysts and Bioprocesses （2021academic year） Prophase - その他
-
Basic of Applied Chemistry and Biotechnology （2021academic year） 1st semester - 金1～2
-
Chemical Engineering 1A （2021academic year） Third semester - 水1,水2
-
Chemical Engineering 1B （2021academic year） Fourth semester - 水1,水2
-
Chemical Engineering 2A （2021academic year） 1st semester - 火1,火2
-
Chemical Engineering 2B （2021academic year） Second semester - 火1,火2
-
Chemical Engineering (Ⅰ) （2021academic year） 3rd and 4th semester - 水1,水2
-
Chemical Engineering (Ⅱ) （2021academic year） 1st and 2nd semester - 火1,火2
-
Internship in Applied Chemistry （2021academic year） Prophase - その他
-
Seminar on Applied Chemistry 1 （2021academic year） Prophase - その他
-
Seminar on Applied Chemistry 2 （2021academic year） Late - その他
-
Basic Applied Chemistry （2021academic year） Late - 月3～4,火3～4
-
Research Works for Master Thesis on Applied Chemistry （2021academic year） Year-round - その他
-
Topics in Material Process 3 （2021academic year） Summer concentration - その他
-
Advanced Materials Chemistry 3 （2021academic year） Concentration - その他
-
Physical Chemistry and Exercises 2 （2021academic year） Third semester - 月3,月4,木3,木4
-
Physical Chemistry 2 （2021academic year） Third semester - 月3,月4,木3,木4
-
Biochemiacl Engineering （2021academic year） Prophase - 木1,木2
-
Seminar in Bioprocess Engineering （2020academic year） Year-round - その他
-
Design of Biocatalysts and Bioprocesses （2020academic year） Late - その他
-
Chemical Engineering 1A （2020academic year） Third semester - 水1,水2
-
Chemical Engineering 1B （2020academic year） Fourth semester - 水1,水2
-
Chemical Engineering 2A （2020academic year） 1st semester - 火1,火2
-
Chemical Engineering 2B （2020academic year） Second semester - 火1,火2
-
Chemical Engineering (Ⅰ) （2020academic year） 3rd and 4th semester - 水1,水2
-
Chemical Engineering (Ⅱ) （2020academic year） 1st and 2nd semester - 火1,火2
-
Internship in Applied Chemistry （2020academic year） Prophase - その他
-
Seminar on Applied Chemistry 1 （2020academic year） Prophase - その他
-
Seminar on Applied Chemistry 2 （2020academic year） Late - その他
-
Basic Applied Chemistry （2020academic year） Late - 月3～4,火3～4
-
Research Works for Master Thesis on Applied Chemistry （2020academic year） Year-round - その他
-
Topics in Material Process 3 （2020academic year） Concentration - その他
-
Topics in Material Process 2 （2020academic year） Summer concentration - その他
-
Advanced Materials Chemistry 7 （2020academic year） Winter concentration - その他
-
Physical Chemistry and Exercises 2 （2020academic year） Third semester - 月3,月4,木3,木4
-
Physical Chemistry 2 （2020academic year） Third semester - 月3,月4,木3,木4
-
Biochemiacl Engineering （2020academic year） Prophase - 木1,木2