Publishing type：Part of collection (book)  

Flexible sol-gel procedure is an excellent route to prepare inorganic-organic hybrids where homogenous precursor solutions yield solid or porous gels and composites in the size from macroscopic ranges (cm, mm) to microscopic (μm) and nanoscopic (nm) ranges. The present chapter first provides short introduction of inorganic-organic hybrids: historical aspects, constitution, and inorganic and organic components to be hybridized as well as their possible biomedical applications. Their biocompatibility is the most significant, and it is discussed in detail in terms of the fixation of various hybrid materials with hard and soft tissues. The primary topics are the behaviors of inorganic species like Si-OH, Ti-OH, calcium ions, and hydroxycarbonate apatite (HCA) deposition, as well as the roles of short peptides like arginine-glycine-aspartate (Arg-Gly-Asp; RGD) and mediating layers. Inorganic-organic layers with Ti-O or P-O favor anti-blood clotting (thrombosis) and polymer-apatite bonding in dentistry. Described are several biomedically applicable porous hybrids among a few silanes, natural polymers (gelatin, chitosan, collagen, and silk fibroin), and synthetic polymers poly(dimethylsilane), poly(lactic acid), and poly (caprolactone). Some are promising to repair damaged nerve systems. Electrospinning, bioprinting, and liquid phase deposition techniques are presented as versatile methods to fabricate fibrous membranes, 3-D architecture, and intermediating oxide layers. Hybrid nanoparticles are introduced as the promising drug delivery and therapeutic vehicles, such as cerasomes (ceramic + liposome), magnetoliposomes, nanomachines, or mesoporous silica. Finally, a few paragraphs stress the significance of quantum dots in the biomedical fields like biosensing or bioimaging.

DOI： 10.1007/978-3-319-32101-1_76

Scopus

researchmap

Publishing type：Research paper (international conference proceedings)  

When solution of a polymer with some inorganic ions is electrospun, the polymer fibrils will be elongated within the spinning direction and those ions are held in the inter-fibril spaces. When those electrospun fibers are calcined, ceramic fibers will be obtained. The present study has attempted to employ such spaces formed in polyvinyl alcohol) (PVA) as nano- or micro-reactor spaces for precipitation of nano-crystalline particles. Exposing the spun PVA mats to ammonia vapor fixed metal ions in the form of hydroxide and, induced apatitic calcium phosphate crystallites when the precursor solution involved calcium and hydrogen orthophosphate ions.

Scopus

researchmap

Publishing type：Research paper (international conference proceedings)  

Silica nanotube sponges and solid silica fiber mats were fabricated using a collagen and electrospun poly(vinylalcohol) as the templates via the sol-gel route. In vitro reassembled collagen fibrils were treated in the Stöber sol-gel precursor mixture of tetraethoxysilane (TEOS), ethanol, water, and ammonia to produce the silica-coated collagen fibrils. The electrospun PVA fiber mats were soaked in the Stöber type as well as acid-catalyzed silica oligomer sol from TEOS. Those were calcined to yield apparently silica nano- or micro-fibers, but transmission and scanning electron microscope observations showed that the Stöber sol treatment resulted in silica nano- or micro-fibers with hollow structure, or nanotubes (silica NTs), while the acid-catalyzed sol yielded solid fibrous mats.

Scopus

researchmap

Publishing type：Research paper (scientific journal)  

Silica fiber mats were fabricated by soaking electro-spun poly(vinyl alcohol) fibril mat in NH4OH-catalyzed (Stöber type) colloidal silica sol and silica oligomer solution from HCl-catalysis of the system tetraethoxysilane, ethanol, and water, followed by appropriate heat treatments. The silica fibrils from the Stöber silica sol were hollow nano-tubes (NT) consisting of nano-sized silica particles, while the silica nanofibers (NF) from the acid-catalyzed sol were solid with minimal porosity. A model was proposed for the NF and NT formation processes. © 2012 The Ceramic Society of Japan. All rights reserved.

DOI： 10.2109/jcersj2.120.520

Scopus

researchmap

Publishing type：Research paper (scientific journal)  

Many studies have been dedicated to the development of scaffolds for improving post-traumatic nerve regeneration. The goal of this study was to assess the effect on nerve regeneration, associating a hybrid chitosan membrane with non-differentiated human mesenchymal stem cells isolated from Wharton's jelly of umbilical cord, in peripheral nerve reconstruction after crush injury. Chromosome analysis on human mesenchymal stem cell line from Wharton's jelly was carried out and no structural alterations were found in metaphase. Chitosan membranes were previously tested in vitro, to assess their ability in supporting human mesenchymal stem cell survival, expansion, and differentiation. For the in vivo testing, Sasco Sprague adult rats were divided in 4 groups of 6 or 7 animals each: Group 1, sciatic axonotmesis injury without any other intervention (Group 1-Crush); Group 2, the axonotmesis lesion of 3 mm was infiltrated with a suspension of 1 250 -1 500 human mesenchymal stem cells (total volume of 50 μL) (Group 2-CrushCell); Group 3, axonotmesis lesion of 3 mm was enwrapped with a chitosan type III membrane covered with a monolayer of non-differentiated human mesenchymal stem cells (Group 3-CrushChitIIICell) and Group 4, axonotmesis lesion of 3 mm was enwrapped with a chitosan type III membrane (Group 4-CrushChitIII). Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks using sciatic functional index, static sciatic index, extensor postural thrust, and withdrawal reflex latency. Stereological analysis was carried out on regenerated nerve fibers. Results showed that infiltration of human mesenchymal stem cells, or the combination of chitosan membrane enwrapment and human mesenchymal stem cell enrichment after nerve crush injury provide a slight advantage to post-traumatic nerve regeneration. Results obtained with chitosan type III membrane alone confirmed that they significantly improve post-traumatic axonal regrowth and may represent a very promising clinical tool in peripheral nerve reconstructive surgery. Yet, umbilical cord human mesenchymal stem cells, that can be expanded in culture and induced to form several different types of cells, may prove, in future experiments, to be a new source of cells for cell therapy, including targets such as peripheral nerve and muscle.

DOI： 10.3969/j.issn.1673-5374.2012.29.002

Scopus

researchmap

Language：Japanese   Publisher：Japanese Society for Engineering Education  

DOI： 10.4307/jsee.72.2_64

researchmap

J-GLOBAL

researchmap

Language：Japanese   Publisher：Japanese Society for Engineering Education  

DOI： 10.4307/jsee.72.3_54

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

Event date： 2023.9.21 - 2023.9.22

Language：English   Presentation type：Oral presentation (invited, special)  

researchmap

Event date： 2022.9.7 - 2022.9.9

Language：English  

researchmap

Event date： 2022.3.10 - 2022.3.12

Presentation type：Oral presentation (invited, special)  

researchmap

Event date： 2012.10.7 - 2012.10.11

Language：English   Presentation type：Poster presentation  

researchmap

Event date： 2010.1.12 - 2010.1.13

Language：English   Presentation type：Oral presentation (general)  

researchmap

researchmap

researchmap

researchmap

researchmap

researchmap

Type：Lecture

researchmap

Type：Other

researchmap

Type：Seminar, workshop

researchmap

Type：Visiting lecture

researchmap

researchmap

researchmap

researchmap

researchmap

researchmap