Updated on 2025/04/24

写真a

 
UEKI Shoko
 
Organization
Scheduled update Associate Professor
Position
Associate Professor
External link

Degree

  • 博士(理学) ( 東京工業大学 )

Research Interests

  • Heterosigma akashiwo

  • ecophysiology

  • cell biology

  • molecular biology

  • microbial ecology

Research Areas

  • Environmental Science/Agriculture Science / Environmental agriculture  / 水圏生命科学

  • Life Science / Molecular biology

  • Life Science / Cell biology

Education

  • Tokyo Institute of Technology   大学院生命理工学研究科  

    1994.4 - 1997.3

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  • University of Tsukuba   環境科学研究科  

    1992.4 - 1994.7

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  • Nagoya University   理学部  

    1988.4 - 1992.3

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    Country: Japan

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Research History

  • Okayama University   Institute of Plant Science and Resources   Associate Professor

    2017.9

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  • Okayama University   Institute of Plant Science and Resources   Assistant Professor

    2011.9 - 2017.7

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  • State University of New York αt Stony Brook   Department of Biochemistry and Cell Biology   Research Assistant Professor

    2007.8 - 2011.8

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    Country:United States

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  • State University of New York at Stony Brook   Department of Biochemistry and Cell Biology

    1998.9 - 2007.7

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    Country:United States

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  • Japan Society for Promotion of Science   東京工業大学 生命理工学部

    1997.4 - 1998.8

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Professional Memberships

 

Papers

  • Differentiation of microbial community in coastal seawater samples before and during a Akashiwo sanguinea (Dinophyceae) bloom in urban area of Antofagasta city (northern Chile) Reviewed

    Jingming Hua, Henry Camerónc, Joaquín I. Rillingd, Marco Camposd, Tay Ruiz-Gild, Mariela A. Gonzaleze, Gonzalo Gajardo, Karen Vergarad, Leonardo Guzmán, Oscar Espinoza-González, Gonzalo Fuenzalida, Carlos Riquelme, Shoko Ueki, Satoshi Nagai, Fumito Maruyama, So Fujiyoshi, Kyoko Yarimizum, Ishara Pereram, Andrés Ávilae, Jacquelinne J. Acuñad, Qian Zhanga, Milko A. Jorquera

    Harmful Algae   2024.12

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    Language:English   Publishing type:Research paper (scientific journal)  

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  • The dataset of de novo assembly and inferred functional annotation of the transcriptome of Heterosigma akashiwo, a bloom-forming, cosmopolitan raphidophyte. Reviewed International journal

    Masanao Sato, Masahide Seki, Yutaka Suzuki, Shoko Ueki

    Data in brief   48   109071 - 109071   2023.6

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Heterosigma akashiwo is a eukaryotic, cosmopolitan, and unicellular alga (class: Raphidophyceae), and produces fish-killing blooms. There is a substantial scientific and practical interest in its ecophysiological characteristics that determine bloom dynamics and its adaptation to broad climate zones. A well-annotated genomic/genetic sequence information enables researchers to characterize organisms using modern molecular technology. In the present study, we conducted H. akashiwo RNA sequencing, a de novo transcriptome assembly of 84,693,530 high-quality deduplicated short-read sequences. Obtained RNA reads were assembled by Trinity assembler and 144,777 contigs were identified with N50 values of 1085. Total 60,877 open reading frames with the length of 150 bp or greater were predicted. For further analyses, top Gene Ontology terms, pfam hits, and blast hits were annotated for all the predicted genes. The raw data were deposited in the NCBI SRA database (BioProject PRJDB6241 and PRJDB15108), and the assemblies are available in NCBI TSA database (ICRV01). The annotation information can be obtained in Dryad and can be accessed via doi: 10.5061/dryad.m0cfxpp56.

    DOI: 10.1016/j.dib.2023.109071

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  • The holobiome of marine harmful algal blooms (HABs): A novel ecosystem-based approach for implementing predictive capabilities and managing decisions Reviewed

    Gonzalo Gajardo, Jesús Morón-López, Karen Vergara, Shoko Ueki, Leonardo Guzmán, Oscar Espinoza-González, Alondra Sandoval, Gonzalo Fuenzalida, Alejandro A. Murillo, Carlos Riquelme, Henry Camerón, Satoshi Nagai, Fumito Maruyama, So Fujiyoshi, Kyoko Yarimizu, Ishara Perera, Mikihiko Kawai, Andrés Ávila, Giovanni Larama, Mariela A. Gonzalez, Joaquín I. Rilling, Marco Campos, Tay Ruiz-Gil, Benjamin Durán-Vinet, Jacquelinne J. Acuña, Milko A. Jorquera

    Environmental Science & Policy   143   44 - 54   2023.5

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.envsci.2023.02.012

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  • Intraspecies variation of the mitochondrial genome: An evaluation for phylogenetic approaches based on the conventional choices of genes and segments on mitogenome. Reviewed International journal

    Jesús Morón-López, Karen Vergara, Masanao Sato, Gonzalo Gajardo, Shoko Ueki

    PloS one   17 ( 8 )   e0273330   2022

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Intraspecies nucleotide sequence variation is a key to understanding the evolutionary history of a species, such as the geographic distribution and population structure. To date, numerous phylogenetic and population genetics studies have been conducted based on the sequences of a gene or an intergenic region on the mitochondrial genome (mtDNA), such as cytochrome c oxidase subunits or the D-loop. To evaluate the credibility of the usage of such 'classic' markers, we compared the phylogenetic inferences based on the analyses of the partial and entire mtDNA sequences. Importantly, the phylogenetic reconstruction based on the short marker sequences did not necessarily reproduce the tree topologies based on the analyses of the entire mtDNA. In addition, analyses on the datasets of various organisms revealed that the analyses based on the classic markers yielded phylogenetic trees with poor confidence in all tested cases compared to the results based on full-length mtDNA. These results demonstrated that phylogenetic analyses based on complete mtDNA sequences yield more insightful results compared to those based on mitochondrial genes and segments. To ameliorate the shortcomings of the classic markers, we identified a segment of mtDNA that may be used as an 'approximate marker' to closely reproduce the phylogenetic inference obtained from the entire mtDNA in the case of mammalian species, which can be utilized to design amplicon-seq-based studies. Our study demonstrates the importance of the choice of mitochondrial markers for phylogenetic analyses and proposes a novel approach to choosing appropriate markers for mammalian mtDNA that reproduces the phylogenetic inferences obtained from full-length mtDNA.

    DOI: 10.1371/journal.pone.0273330

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  • Suitcase Lab: new, portable, and deployable equipment for rapid detection of specific harmful algae in Chilean coastal waters Reviewed

    So Fujiyoshi, Kyoko Yarimizu, Yohei Miyashita, Joaquín Rilling, Jacquelinne J. Acuña, Shoko Ueki, Gonzalo Gajardo, Oscar Espinoza-González, Leonardo Guzmán, Milko A. Jorquera, Satoshi Nagai, Fumito Maruyama

    Environmental Science and Pollution Research   2020.11

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>Phytoplankton blooms, including harmful algal blooms (HABs), have serious impacts on ecosystems, public health, and productivity activities. Rapid detection and monitoring of marine microalgae are important in predicting and managing HABs. We developed a toolkit, the Suitcase Lab, to detect harmful algae species in the field. We demonstrated the Suitcase Lab’s capabilities for sampling, filtration, DNA extraction, and loop-mediated isothermal amplification (LAMP) detection in cultured <italic>Alexandrium catenella</italic> cells as well as Chilean coastal waters from four sites: Repollal, Isla García, Puerto Montt, and Metri. A LAMP assay using the Suitcase Lab in the field confirmed microscopic observations of <italic>A. catenella</italic> in samples from Repollal and Isla García. The Suitcase Lab allowed the rapid detection of <italic>A. catenella</italic>, within 2 h from the time of sampling, even at a single cell per milliliter concentrations, demonstrating its usefulness for quick and qualitative on-site diagnosis of target toxic algae species. This method is applicable not only to detecting harmful algae but also to other field studies that seek a rapid molecular diagnostic test.

    DOI: 10.1007/s11356-020-11567-5

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    Other Link: http://link.springer.com/article/10.1007/s11356-020-11567-5/fulltext.html

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Presentations

  • Study on bacterivory and nutrition acquisition of Heterosigma akashiwo

    Shoko Ueki

    2024.12.4 

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    Event date: 2024.12.3 - 2024.12.4

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • リン⽋乏状態における海洋細菌による⾚潮原因藻の増殖促進機構の解明

    福山 誠也, 宇佐美 文子, 小原 静香, 隠塚 俊光, 近藤 健, 小池 和彦, 植木 尚子

    おかやまバイオアクティブ研究会  2024.6.6 

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    Event date: 2024.6.6

    Presentation type:Poster presentation  

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  • A marine bacterium promotes the growth of a bloom-forming phytoplankton under phosphorus-depletion

    Seiya Fukuyama, Fumiko Usami, Shizuka Ohara, Toshimitsu Onduka, Ken Kondo, Kazuhiko Koike, Shoko Ueki

    2023.11.28 

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    Event date: 2023.11.28 - 2023.11.30

    Language:English   Presentation type:Poster presentation  

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  • Characterization of marine bacteria that support growth of Heterosigma akashiwo under phosphate-limiting conditions

    Shoko Ueki

    International Conference on Harmful Algae 2023  2023.11.6 

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    Event date: 2023.11.6 - 2023.11.10

    Language:English   Presentation type:Oral presentation (general)  

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  • Characterization of Marine Bacteria That Support Growth of Harmful Algae Under Nutritionally Limiting Conditions. Invited

    Shoko Ueki

    Annual Meeting of Society of Microbiology in Chile  2021.11.30 

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    Event date: 2021.11.29 - 2021.11.30

    Language:English   Presentation type:Oral presentation (invited, special)  

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Awards

  • 長瀬研究振興賞

    2013.3   公益財団法人長瀬科学技術振興財団  

    植木尚子

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Research Projects

  • ラフィド藻ヘテロシグマをモデルとした赤潮形成を支える増殖加速と代謝機構の解明

    Grant number:25K02346  2025.04 - 2029.03

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    植木 尚子

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    Authorship:Principal investigator 

    Grant amount:\18720000 ( Direct expense: \14400000 、 Indirect expense:\4320000 )

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  • 赤潮原因藻ヘテロシグマの赤潮形成期における増殖加速機構の解明を目指した研究

    Grant number:24K21840  2024.06 - 2026.03

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

    植木 尚子

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    Authorship:Principal investigator 

    Grant amount:\6370000 ( Direct expense: \4900000 、 Indirect expense:\1470000 )

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  • Characterization of a novel bloom-forming mechanism based on interaction between astaxanthin-producing marine bacteria and a bloom-forming alga

    Grant number:21K19148  2021.07 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    植木 尚子

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    Authorship:Principal investigator 

    Grant amount:\6240000 ( Direct expense: \4800000 、 Indirect expense:\1440000 )

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  • Characterization of the nutritional complementation by marine bacteria in the process of algal bloom formation

    Grant number:21H02285  2021.04 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    植木 尚子, 隠塚 俊満, 小原 静夏, 近藤 健

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    Authorship:Principal investigator 

    Grant amount:\17160000 ( Direct expense: \13200000 、 Indirect expense:\3960000 )

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  • Effect of anthropogenic chemicals on primary production in coastal areas: elucidation of combined effects of limiting nutrient and strong light

    Grant number:20H03067  2020.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    隠塚 俊満, 小池 一彦, 植木 尚子, 小原 静夏

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    Authorship:Coinvestigator(s) 

    Grant amount:\17550000 ( Direct expense: \13500000 、 Indirect expense:\4050000 )

    和歌山市から広島県呉市にかけて陸域から瀬戸内海本州側の採水調査を実施し,昨年度確立した人為起源化学物質(AC)である抗生物質および除草剤の分析法を用いて環境水中濃度を測定した。その結果,広島県三原市,兵庫県姫路市,大阪府忠岡町から合計濃度100-250ng/Lと,比較的高濃度でACが検出された。これらの調査地点は下水処理場や下水汚泥処理場から直線距離で概ね500m未満であり,これらの処理水の影響が推定された。
    瀬戸内海で検出されるACと本海域で優占する植物プランクトン種を用いて影響試験を行った。除草剤ジウロンとブロマシルの50%光合成阻害濃度(EC50-ETR)は珪藻2種よりも鞭毛藻2種で低くかった一方で,50%増殖阻害濃度(EC50-μ)は種間差がほぼ無いか,寧ろ珪藻2種の方が低かった。これは珪藻が除草剤による光合成阻害によって増殖も阻害される一方で,鞭毛藻は光合成を阻害されても増殖速度をある程度維持できることを示唆した。抗生物質クラリスロマイシン,クリンダマイシン,アジスロマイシンのEC50-ETRおよびEC50-μはどちらも珪藻2種の方が鞭毛藻2種よりも低く,光合成阻害と増殖阻害で同じ傾向を示した。これは抗生物質が光合成だけでなく他の代謝経路(呼吸など)も阻害する可能性を示唆した。植物プランクトン種ごとに比較すると,何れの指標を用いても珪藻Skeletonema costatumは抗生物質に対する感受性が特に高かった。
    2021年6-10月に月1回,広島県福山市の一級河川芦田川河口と河口近くに位置する田尻港の表層水を採取し,現場海水の化学物質を等倍,5倍,10倍,50倍に濃縮した濃縮液を珪藻S. costatumに曝露し培養試験を行った。芦田川河口試水では6月の10, 50倍,8月の50倍で,田尻港試水では7, 8, 10月の50倍濃縮区で50%以上増殖が阻害された。

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Other research activities

  • Heterosigma akashiwo transcriptome assembly and annotation

    2023.03

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    Transcriptome shotgun assembly (TSA) can be obateinded from https://www.ncbi.nlm.nih.gov/Traces/wgs/ICRV01
    Annotation for the TSAa can be obtained from 10.5061/dryad.m0cfxpp56

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  • ラボHP

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    https://www.rib.okayama-u.ac.jp/inv/index-j.html

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Class subject in charge

  • Topics in Plant Microbe Interactions (2024academic year) Prophase  - その他

  • Topics in Plant Microbe Interactions (2024academic year) Prophase  - その他

  • Seminar in Plant-Environmental Microbiology (2024academic year) Prophase  - その他

  • Seminar in Plant-Environmental Microbiology (2024academic year) Late  - その他

  • Seminar in Plant-Environmental Microbiology (2024academic year) Prophase  - その他

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Social Activities

  • Ohara Summer Science Internship

    Role(s):Planner, Organizing member

    Institute of Plant Science and Resources, Okayama University  2023.8.29 - 2023.9.1

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    Audience: College students

    Type:Other

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  • 大原サマーサイエンスインターンシップ

    Role(s):Planner, Organizing member

    岡山大学 資源植物科学研究所  大原サマーサイエンスインターンシップ  2022.9.5 - 2022.9.8

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    Type:Other

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Media Coverage

  • Dra Shoko Ueki, de la Univ. de Okayama y del Proyecto MACH, imparte charla sobre las algas nocivas. TV or radio program

    Colegio San Mateo de la Compañía de Jesús  Dra Shoko Ueki, de la Univ. de Okayama y del Proyecto MACH, imparte charla sobre las algas nocivas.  2023.3

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