Updated on 2025/08/26

写真a

 
WATANABE Kazunori
 
Organization
Faculty of Interdisciplinary Science and Engineering in Health Systems Associate Professor
Position
Associate Professor
Homepage
https://www.okayama-u.ac.jp/user/ohtsuki/
Profile

保有資格：

第１種放射線取扱主任者

第１種衛生工学衛生管理者

External link

Degree

  • Doctor (Engineering ( Ehime University )

Research Interests

  • molecular and cell biology

  • 分子細胞生物学

  • ストレス顆粒形成

  • 生化学

  • ストレス応答

  • RNA分解

  • 相分離

Research Areas

  • Life Science / Functional biochemistry

  • Life Science / Molecular biology

Research History

  • Okayama University   学術研究院ヘルスシステム統合科学学域   Associate Professor

    2023.4

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  • 岡山大学学術研究院ヘルスシステム統合科学学域   助教

    2021.4 - 2023.3

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  • 岡山大学大学院ヘルスシステム統合科学研究科   助教

    2019.4 - 2021.3

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  • 岡山大学自然科学研究科 助教

    2012 - 2019.3

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  • - Assistant Professor,Graduate School of Natural Science and Technology,Okayama University

    2012

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Professional Memberships

Committee Memberships

  • 日本ハイパーサーミア学会   編集委員会委員  

    2024.4   

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    Committee type：Academic society

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  • 日本ハイパーサーミア学会   代議員  

    2023.9   

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Papers

  • Mechanistic Insights Into Oxidative Response of Heat Shock Factor 1 Condensates Reviewed

    Soichiro Kawagoe, Motonori Matsusaki, Takuya Mabuchi, Yuto Ogasawara, Kazunori Watanabe, Koichiro Ishimori, Tomohide Saio

    JACS Au   5 ( 2 )   606 - 617   2025.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/jacsau.4c00578

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  • Novel Drug Delivery Particles Can Provide Dual Effects on Cancer “Theranostics” in Boron Neutron Capture Therapy Reviewed

    Abdul Basith Fithroni, Haruki Inoue, Shengli Zhou, Taufik Fatwa Nur Hakim, Takashi Tada, Minoru Suzuki, Yoshinori Sakurai, Manabu Ishimoto, Naoyuki Yamada, Rani Sauriasari, Wolfgang A. G. Sauerwein, Kazunori Watanabe, Takashi Ohtsuki, Eiji Matsuura

    Cells   14 ( 1 )   60 - 60   2025.1

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Boron (B) neutron capture therapy (BNCT) is a novel non-invasive targeted cancer therapy based on the nuclear capture reaction 10B (n, alpha) 7Li that enables the death of cancer cells without damaging neighboring normal cells. However, the development of clinically approved boron drugs remains challenging. We have previously reported on self-forming nanoparticles for drug delivery consisting of a biodegradable polymer, namely, “AB-type” Lactosome® nanoparticles (AB-Lac particles)- highly loaded with hydrophobic B compounds, namely o-Carborane (Carb) or 1,2-dihexyl-o-Carborane (diC6-Carb), and the latter (diC6-Carb) especially showed the “molecular glue” effect. Here we present in vivo and ex vivo studies with human pancreatic cancer (AsPC-1) cells to find therapeutically optimal formulas and the appropriate treatment conditions for these particles. The biodistribution of the particles was assessed by the tumor/normal tissue ratio (T/N) in terms of tumor/muscle (T/M) and tumor/blood (T/B) ratios using near-infrared fluorescence (NIRF) imaging with indocyanine green (ICG). The in vivo and ex vivo accumulation of B delivered by the injected AB-Lac particles in tumor lesions reached a maximum by 12 h post-injection. Irradiation studies conducted both in vitro and in vivo showed that AB-Lac particles-loaded with either 10B-Carb or 10B-diC6-Carb significantly inhibited the growth of AsPC-1 cancer cells or strongly inhibited their growth, with the latter method being significantly more effective. Surprisingly, a similar in vitro and in vivo irradiation study showed that ICG-labeled AB-Lac particles alone, i.e., without any 10B compounds, also revealed a significant inhibition. Therefore, we expect that our ICG-labeled AB-Lac particles-loaded with 10B compound(s) may be a novel and promising candidate for providing not only NIRF imaging for a practical diagnosis but also the dual therapeutic effects of induced cancer cell death, i.e., “theranostics”.

    DOI： 10.3390/cells14010060

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  • Photochemical internalization of mRNA using a photosensitizer and nucleic acid carriers Reviewed

    Hayaki Maemoto, Ryohei Suzaki, Kazunori Watanabe, Keiji Itaka, Takashi Ohtsuki

    European Journal of Medicinal Chemistry Reports   13   100242 - 100242   2024.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.ejmcr.2024.100242

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  • Configuration of two cysteine residues in a ring within a stapled Bim peptide affects the secondary structure and apoptotic activity Reviewed

    Shengli Zhou, Fuka Nishimura, Kazuhaya Wada, Kaho Fujii, Takeshi Kondo, Kazunori Watanabe, Yoshitane Imai, Takashi Ohtsuki, Mizuki Kitamatsu

    Bioorganic & Medicinal Chemistry Letters   112   129915 - 129915   2024.11

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.bmcl.2024.129915

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  • In Vitro Study of Tumor-Homing Peptide-Modified Magnetic Nanoparticles for Magnetic Hyperthermia Reviewed

    Shengli Zhou, Kaname Tsutsumiuchi, Ritsuko Imai, Yukiko Miki, Anna Kondo, Hiroshi Nakagawa, Kazunori Watanabe, Takashi Ohtsuki

    Molecules   29 ( 11 )   2632 - 2632   2024.6

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Cancer cells have higher heat sensitivity compared to normal cells; therefore, hyperthermia is a promising approach for cancer therapy because of its ability to selectively kill cancer cells by heating them. However, the specific and rapid heating of tumor tissues remains challenging. This study investigated the potential of magnetic nanoparticles (MNPs) modified with tumor-homing peptides (THPs), specifically PL1 and PL3, for tumor-specific magnetic hyperthermia therapy. The synthesis of THP-modified MNPs involved the attachment of PL1 and PL3 peptides to the surface of the MNPs, which facilitated enhanced tumor cell binding and internalization. Cell specificity studies revealed an increased uptake of PL1- and PL3-MNPs by tumor cells compared to unmodified MNPs, indicating their potential for targeted delivery. In vitro hyperthermia experiments demonstrated the efficacy of PL3-MNPs in inducing tumor cell death when exposed to an alternating magnetic field (AMF). Even without exposure to an AMF, an additional ferroptotic pathway was suggested to be mediated by the nanoparticles. Thus, this study suggests that THP-modified MNPs, particularly PL3-MNPs, hold promise as a targeted approach for tumor-specific magnetic hyperthermia therapy.

    DOI： 10.3390/molecules29112632

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  • Self-assembly of Peptide Amphiphiles with Alkyl Groups for siRNA Delivery Reviewed

    Taufik F.N. Hakim, Kazunori Watanabe, Shomu Fujimoto, Mizuki Kitamatsu, Takashi Ohtsuki

    Chemistry Letters   2023.9

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    Publishing type：Research paper (scientific journal)   Publisher：The Chemical Society of Japan  

    DOI： 10.1246/cl.230302

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  • Photo-dependent cytosolic delivery of shRNA into a single blastomere in a mouse embryo. Reviewed International journal

    Yuka Ikawa, Takuya Wakai, Hiroaki Funahashi, Tet Htut Soe, Kazunori Watanabe, Takashi Ohtsuki

    Scientific reports   13 ( 1 )   13050 - 13050   2023.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    Single-cell-specific delivery of small RNAs, such as short hairpin RNA (shRNA) and small noncoding RNAs, allows us to elucidate the roles of specific upregulation of RNA expression and RNAi-mediated gene suppression in early embryo development. The photoinduced cytosolic dispersion of RNA (PCDR) method that we previously reported can introduce small RNAs into the cytosol of photoirradiated cells and enable RNA delivery into a single-cell in a spatiotemporally specific manner. However, the PCDR method has only been applied to planer cultured cells and not to embryos. This study demonstrated that the PCDR method can be utilized for photo-dependent cytosolic shRNA delivery into a single blastomere and for single blastomere-specific RNA interference in mouse embryos. Our results indicate that PCDR is a promising approach for studying the developmental process of early embryogenesis.

    DOI： 10.1038/s41598-023-40361-9

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  • FRET probe for detecting two mutations in one EGFR mRNA. Reviewed International journal

    Myat Thu, Kouta Yanai, Hajime Shigeto, Shohei Yamamura, Kazunori Watanabe, Takashi Ohtsuki

    The Analyst   148 ( 11 )   2626 - 2632   2023.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    Technologies for visualizing and tracking RNA are essential in molecular biology, including in disease-related fields. In this study, we propose a novel probe set (DAt-probe and T-probe) that simultaneously detects two mutations in the same RNA using fluorescence resonance energy transfer (FRET). The DAt-probe carrying the fluorophore Atto488 and the quencher Dabcyl were used to detect a cancer mutation (exon19del), and the T-probe carrying the fluorophore Tamra was used to detect drug resistance mutations (T790M) in epidermal growth factor receptor (EGFR) mRNA. These probes were designed to induce FRET when both mutations were present in the mRNA. Gel electrophoresis confirmed that the two probes could efficiently bind to the mutant mRNA. We measured the FRET ratios using wild-type and double-mutant RNAs and found a significant difference between them. Even in living cells, the FRET probe could visualize mutant RNA. As a result, we conclude that this probe set provides a method for detecting two mutations in the single EGFR mRNA via FRET.

    DOI： 10.1039/d3an00554b

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  • Photochemical internalizationに基づく光依存的細胞質内RNA導入法の副作用低減 Reviewed

    Akinari Bando, Kazunori Watanabe, Takashi Ohtsuki

    The Journal of Japan Society for Laser Surgery and Medicine   44 ( 1 )   62 - 68   2023.4

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Laser Surgery and Medicine  

    DOI： 10.2530/jslsm.jslsm-44_0004

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  • Ultrasound-dependent RNAi using TatU1A-rose bengal conjugate. Reviewed International journal

    Nanako Sumi, Shota Nagahiro, Eiji Nakata, Kazunori Watanabe, Takashi Ohtsuki

    Bioorganic & medicinal chemistry letters   68   128767 - 128767   2022.7

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    Tat-U1A-rose bengal conjugate (TatU1A-RB) was prepared as an ultrasound-sensitive RNA carrier molecule. This molecule consists of Tat cell-penetrating peptide, U1A RNA-binding protein, and rose bengal as a sonosensitizer. We demonstrated that TatU1A-RB delivered RNA via the endocytosis pathway, which was followed by ultrasound-dependent endosomal escape and cytosolic dispersion of the RNA. A short hairpin RNA (shRNA) delivered by TatU1A-RB mediated RNA interference (RNAi) ultrasound-dependently. Even by ultrasound irradiation through blood cells, RNAi could be induced with TatU1A-RB and the shRNA. This ultrasound-dependent cytosolic RNA delivery method will serve as the basis for a new approach to nucleic acid therapeutics.

    DOI： 10.1016/j.bmcl.2022.128767

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  • Fluorophore-PNA-Quencher/Quencher-DNA probe for miRNA detection. Reviewed International journal

    Kentaro Tabara, Kazunori Watanabe, Hajime Shigeto, Shohei Yamamura, Takamasa Kishi, Mizuki Kitamatsu, Takashi Ohtsuki

    Bioorganic & medicinal chemistry letters   51   128359 - 128359   2021.9

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    Micro RNAs (miRNAs) are involved in a variety of biological functions and are attracting attention as diagnostic and prognostic markers for various diseases. Highly sensitive RNA detection methods are required to determine miRNA expression levels and intracellular localization. In this study, we designed new double-stranded peptide nucleic acid (PNA)/DNA probes consisting of a fluorophore-PNA-quencher (fPq) and a quencher-DNA (qD) for miR-221 detection. We optimized the fPq structure, PNA-DNA hybrid length, and hybrid position. The resultant fPq-2/qD-6b probe was a 6-bp hybrid probe with a 10-base fPq and a 6-base qD. The signal-to-background ratios of the probes showed that fPq-2/qD-6b had a higher target sensitivity than fPq (PNA beacon)-type and fP/qD-type probes. The results of the detection limit and target specificity indicate that the fPq/qD probe is promising for RNA detection in both cells and cell extracts as well as for miRNA diagnosis.

    DOI： 10.1016/j.bmcl.2021.128359

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  • Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer Reviewed

    Kazunori Watanabe, Tomoko Nawachi, Ruriko Okutani, Takashi Ohtsuki

    Scientific Reports   11 ( 1 )   14936   2021.7

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    <title>Abstract</title>Methods to spatially induce apoptosis are useful for cancer therapy. To control the induction of apoptosis, methods using light, such as photochemical internalization (PCI), have been developed. We hypothesized that photoinduced delivery of microRNAs (miRNAs) that regulate apoptosis could spatially induce apoptosis. In this study, we identified pre-miR-664a as a novel apoptosis-inducing miRNA via mitochondrial apoptotic pathway. Further, we demonstrated the utility of photoinduced cytosolic dispersion of RNA (PCDR), which is an intracellular RNA delivery method based on PCI. Indeed, apoptosis is spatially regulated by pre-miR-664a and PCDR. In addition, we found that apoptosis induced by pre-miR-664a delivered by PCDR was more rapid than that by lipofection. These results suggest that pre-miR-664a is a nucleic acid drug candidate for cancer therapy and PCDR and pre-miR-664a-based strategies have potential therapeutic uses for diseases affecting various cell types.

    DOI： 10.1038/s41598-021-94249-7

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    Other Link： http://www.nature.com/articles/s41598-021-94249-7

  • Inhibition of HSF1 and SAFB Granule Formation Enhances Apoptosis Induced by Heat Stress Reviewed

    Kazunori Watanabe, Takashi Ohtsuki

    International Journal of Molecular Sciences   22 ( 9 )   4982 - 4982   2021.5

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/ijms22094982

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  • Lactosome-Conjugated siRNA Nanoparticles for Photo-Enhanced Gene Silencing in Cancer Cells Reviewed

    Melissa Siaw Han Lim, Yuki Nishiyama, Takashi Ohtsuki, Kazunori Watanabe, Hirotsugu Kobuchi, Kazuko Kobayashi, Eiji Matsuura

    Journal of Pharmaceutical Sciences   110 ( 4 )   1788 - 1798   2021.4

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    DOI： 10.1016/j.xphs.2021.01.026

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  • Minimization of apoptosis-inducing CPP-Bim peptide. Reviewed International journal

    Shengli Zhou, Kazunori Watanabe, Seiichiro Koide, Mizuki Kitamatsu, Takashi Ohtsuki

    Bioorganic & medicinal chemistry letters   36   127811 - 127811   2021.3

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    Pro-apoptotic peptides may be promising agents for cancer therapy owing to their ability to induce apoptosis in cancer cells. TatBim, a fusion peptide of Tat cell-penetrating peptide (CPP) and the BH3 domain derived from Bim apoptosis-inducing protein, is a pro-apoptotic peptide. In this study, based on the TatBim sequence, we attempted to minimize the CPP-Bim peptide while retaining apoptosis-inducing activity. The CPP and Bim parts were systematically shortened, and the pro-apoptotic activities of the shortened peptides were examined. We obtained TatBim-N1C2 and R8Bim-N1C2 as minimized peptides with efficient apoptotic activity. These peptides may have potential applications in future biomedical studies, such as cancer therapeutics.

    DOI： 10.1016/j.bmcl.2021.127811

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  • Fluorescence lifetime probes for detection of RNA degradation. Reviewed International journal

    Riku Hirata, Kazutaka Hirakawa, Naotaka Shimada, Kazunori Watanabe, Takashi Ohtsuki

    The Analyst   146 ( 1 )   277 - 282   2021.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    To investigate RNA degradation in live cells, detection methods that do not require RNA extraction from cells are necessary. In this study, we examined the utility of fluorescence lifetime measurements using a probe attached to the end of an RNA molecule for detecting RNA degradation. We optimized a short fluorescein-labeled RNA sequence whose fluorescence lifetime varied significantly before and after degradation. The selected HHG-fluorescein sequence (H = U, C, or A) is a promising RNA labeling unit (fluorescence lifetime probe) for live cell imaging of RNA degradation.

    DOI： 10.1039/d0an01230k

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  • Cell cycle dependence of apoptosis photo-triggered using peptide-photosensitizer conjugate. Reviewed International journal

    Hyungjin Kim, Sho Watanabe, Mizuki Kitamatsu, Kazunori Watanabe, Takashi Ohtsuki

    Scientific reports   10 ( 1 )   19087 - 19087   2020.11

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    Investigation of the relevance between cell cycle status and the bioactivity of exogenously delivered biomacromolecules is hindered by their time-consuming cell internalization and the cytotoxicity of transfection methods. In this study, we addressed these problems by utilizing the photochemical internalization (PCI) method using a peptide/protein-photosensitizer conjugate, which enables immediate cytoplasmic internalization of the bioactive peptides/proteins in a light-dependent manner with low cytotoxicity. To identify the cell-cycle dependent apoptosis, a TatBim peptide-photosensitizer conjugate (TatBim-PS) with apoptotic activity was photo-dependently internalized into HeLa cells expressing a fluorescent ubiquitination-based cell cycle indicator (Fucci2). Upon irradiation, cytoplasmic TatBim-PS internalization exceeded 95% for all cells classified in the G1, S, and G2/M cell cycle phases with no significant differences between groups. TatBim-PS-mediated apoptosis was more efficiently triggered by photoirradiation in the G1/S transition than in the G1 and S/G2/M phases, suggesting high sensitivity of the former phase to Bim-induced apoptosis. Thus, the cell cycle dependence of Bim peptide-induced apoptosis was successfully investigated using Fucci2 indicator and the PCI method. Since PCI-mediated cytoplasmic internalization of peptides is rapid and does not span multiple cell cycle phases, the Fucci-PCI method constitutes a promising tool for analyzing the cell cycle dependence of peptides/protein functions.

    DOI： 10.1038/s41598-020-76100-7

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  • Endosomal Escape of Peptide-Photosensitizer Conjugates Is Affected by Amino Acid Sequences near the Photosensitizer. Reviewed International journal

    Yuichi Miyoshi, Maho Kadono, Shigetoshi Okazaki, Ayano Nishimura, Mizuki Kitamatsu, Kazunori Watanabe, Takashi Ohtsuki

    Bioconjugate chemistry   31 ( 3 )   916 - 922   2020.3

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    Cell-penetrating peptides (CPPs) are widely used for the intracellular delivery of peptides and proteins, but CPP fusion peptides and proteins are often transported by endocytosis and trapped in endosomes. Photochemical internalization (PCI) is a method for the endosomal escape of the trapped peptide or protein and release into the cytosol using light and photosensitizers. In PCI, endosomal membranes are thought to be destabilized by singlet oxygen (1O2) photogenerated from photosensitizers localized in endosomes. We previously developed CPP-cargo-photosensitizer (PS) conjugates able to photodependently enter the cytosol via the PCI mechanism. For example, TatU1A-PS (a covalent complex of Tat [CPP], U1A RNA-binding protein [cargo], and PS) can photodependently deliver RNAs into the cytosol, and TatBim-PS (a covalent complex of Tat, Bim [cargo], and PS) can photoinduce apoptosis in mammalian cells. However, for many newly created conjugates, the induction of PCI has been insufficient. We hypothesized that the amino acid linker sequence (XX) adjacent to the photosensitizer is an important determinant of PCI efficiency. In this study, using CPP-cargo-XX-PS platforms, we examined the relationship between PCI efficiency and the linker amino acid sequence near the photosensitizer. We found that hydrophobic FF and LL linkers enhanced the PCI efficiencies of both TatBim-XX-PS and TatU1A-XX-PS. The effectiveness of the linker depended, in part, on both the cargo moiety and the photosensitizer. These results may guide the design of CPP-cargo-PS conjugates conferring broad target functions for PCI and photodynamic therapy.

    DOI： 10.1021/acs.bioconjchem.0c00046

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  • Relation of Photochemical Internalization to Heat, pH and Ca2+ Ions. Reviewed International journal

    Tet Htut Soe, Tomotaka Nanjo, Kazunori Watanabe, Takashi Ohtsuki

    Photochemistry and photobiology   95 ( 6 )   1395 - 1402   2019.11

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    The inefficient endosomal escape of drugs or macromolecules is a major obstacle to achieving successful delivery to therapeutic targets. An efficient approach to circumvent this barrier is photochemical internalization (PCI), which uses light and photosensitizers for endosomal escape of the delivered macromolecules. The PCI mechanism is related to photogenerated singlet oxygen, but the mechanism is still unclear. In this study, we examined the relation of PCI to heat, pH and Ca2+ ions using cell penetrating peptide (CPP)-cargo-photosensitizer (Alexa546 or Alexa633) conjugates. A cell temperature changing experiment demonstrated that heat (thermal mechanism) does not significantly contribute to the photoinduced endosomal escape. Inhibition of V-ATPase proton pump activity and endosomal pH upregulation indicated that PCI-mediated endosomal escape needs endosomal acidification prior to photoirradiation. Imaging of the CPP-cargo-photosensitizer and Ca2+ ions during photostimulation showed that intracellular calcium increase is not the cause of the endosomal escape of the complex. The increment is mainly due to Ca2+ influx. These findings show the importance of extra- and intracellular milieu conditions in the PCI mechanism and enrich our understanding of PCI-related changes in cell.

    DOI： 10.1111/php.13146

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  • In-stem molecular beacon targeted to a 5'-region of tRNA inclusive of the D arm that detects mature tRNA with high sensitivity. Reviewed International journal

    Yuichi Miyoshi, Takashi Ohtsuki, Hiromu Kashida, Hiroyuki Asanuma, Kazunori Watanabe

    PloS one   14 ( 1 )   e0211505   2019

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Cellular functions are regulated by the up- and down-regulation and localization of RNA molecules. Therefore, many RNA detection methods have been developed to analyze RNA levels and localization. Molecular beacon (MB) is one of the major methods for quantitative RNA detection and analysis of RNA localization. Most oligonucleotide-based probes, including MB, are designed to target a long flexible region on the target RNA molecule, e.g., a single-stranded region. Recently, analyses of tRNA localization and levels became important, as it has been shown that environmental stresses and chemical reagents induce nuclear accumulation of tRNA and tRNA degradation in mammalian cells. However, tRNA is highly structured and does not harbor any long flexible regions. Hence, only a few methods are currently available for detecting tRNA. In the present study, we attempted to detect elongator tRNAMet (eMet) and initiator tRNAMet (iMet) by using an in-stem molecular beacon (ISMB), characterized by more effective quenching and significantly higher sensitivity than those of conventional MB. We found that ISMB1 targeted a 5'- region that includes the D arm of tRNA and that it detected eMet and iMet transcripts as well as mature eMet with high sensitivity. Moreover, the analysis revealed that the formation of the ISMB/tRNA transcript complex required more time than the formation of an ISMB/unstructured short RNA complex. These results suggest that ISMB-based tRNA detection can be a useful tool for various biological and medical studies.

    DOI： 10.1371/journal.pone.0211505

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  • Red and Near-Infrared Light-Directed Cytosolic Delivery of Two Different RNAs Using Photosensitive RNA Carriers. Reviewed International journal

    Kaori Shiraga, Tet Htut Soe, Sho Matsumoto, Kazunori Watanabe, Takashi Ohtsuki

    Bioconjugate chemistry   29 ( 9 )   3174 - 3179   2018.9

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    Many cellular events are thought to be controlled by the temporal upregulation of multiple RNAs; the timing of the upregulation of these RNAs is not always the same. In this study, we first show that our light-directed intracellular RNA delivery method induced high concentrations of RNA in a short period. This effect was beneficial for the temporal control of cellular events by functional RNAs. Next, we stimulated the short-term upregulation of two different RNAs at different time points. Cytosolic delivery of a first RNA was induced by red light; thereafter, cytosolic delivery of a second RNA was induced by near-infrared light. The time difference between the introduction of the first and second RNA can be short (0.5-4 h) or long (>8 h). This strategy shows the potential for future applications of the deliberate control of time-dependent RNA concentration to guide various cellular functions by multiple RNAs.

    DOI： 10.1021/acs.bioconjchem.8b00487

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  • MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells. Reviewed International journal

    Kazunori Watanabe, Ryuhei Yamaji, Takashi Ohtsuki

    Genes to cells : devoted to molecular & cellular mechanisms   23 ( 3 )   225 - 233   2018.3

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Blackwell Publishing Ltd  

    DOI： 10.1111/gtc.12559

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  • Ultrasound-dependent cytoplasmic internalization of a peptide-sonosensitizer conjugate. Reviewed International journal

    Yuki Inaba, Kazunori Watanabe, Mizuki Kitamatsu, Eiji Nakata, Atsushi Harada, Takashi Ohtsuki

    Bioorganic & medicinal chemistry   25 ( 15 )   4212 - 4217   2017.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bmc.2017.06.024

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  • Detection of small, highly structured RNAs using molecular beacons Reviewed

    J. Li, C. Xu, N. Shimada, Y. Miyoshi, K. Watanabe, W. Cong, T. Ohtsuki

    ANALYTICAL METHODS   9 ( 20 )   2971 - 2976   2017.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/c7ay00341b

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  • Phototriggered protein syntheses by using (7-diethylaminocoumarin-4-yl)methoxycarbonyl-caged aminoacyl tRNAs. Reviewed International journal

    Takashi Ohtsuki, Shigeto Kanzaki, Sae Nishimura, Yoshio Kunihiro, Masahiko Sisido, Kazunori Watanabe

    Nature communications   7   12501 - 12501   2016.8

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/ncomms12501

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  • Photoinduced apoptosis using a peptide carrying a photosensitizer. Reviewed International journal

    Kazunori Watanabe, Hayato Fujiwara, Mizuki Kitamatsu, Takashi Ohtsuki

    Bioorganic & medicinal chemistry letters   26 ( 13 )   3115 - 3118   2016.7

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bmcl.2016.04.091

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  • Effects of polyamines from Thermus thermophilus, an extreme-thermophilic eubacterium, on tRNA methylation by tRNA (Gm18) methyltransferase (TrmH). Reviewed International journal

    Hiroyuki Hori, Yusuke Terui, Chisato Nakamoto, Chikako Iwashita, Anna Ochi, Kazunori Watanabe, Tairo Oshima

    Journal of biochemistry   159 ( 5 )   509 - 17   2016.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/jb/mvv130

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  • Enhanced cellular uptake of lactosomes using cell-penetrating peptides. Reviewed International journal

    Akiya Akahoshi, Eiji Matsuura, Eiichi Ozeki, Hayato Matsui, Kazunori Watanabe, Takashi Ohtsuki

    Science and technology of advanced materials   17 ( 1 )   245 - 252   2016

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/14686996.2016.1178056

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  • The molecular mechanism of photochemical internalization of cell penetrating peptide-cargo-photosensitizer conjugates. Reviewed International journal

    Takashi Ohtsuki, Shunya Miki, Shouhei Kobayashi, Tokuko Haraguchi, Eiji Nakata, Kazutaka Hirakawa, Kensuke Sumita, Kazunori Watanabe, Shigetoshi Okazaki

    Scientific reports   5   18577 - 18577   2015.12

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    DOI： 10.1038/srep18577

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  • Photo-dependent protein biosynthesis using a caged aminoacyl-tRNA. Reviewed International journal

    Akiya Akahoshi, Yoshio Doi, Masahiko Sisido, Kazunori Watanabe, Takashi Ohtsuki

    Bioorganic & medicinal chemistry letters   24 ( 23 )   5369 - 72   2014.12

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    DOI： 10.1016/j.bmcl.2014.10.053

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  • mTOR regulates the nucleoplasmic diffusion of Xrn2 under conditions of heat stress. Reviewed International journal

    Kazunori Watanabe, Kenichi Ijiri, Takashi Ohtsuki

    FEBS letters   588 ( 18 )   3454 - 60   2014.9

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.febslet.2014.08.003

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  • Near-infrared light-directed RNAi using a photosensitive carrier molecule. Reviewed International journal

    Yuka Matsushita-Ishiodori, Mika Morinaga, Kazunori Watanabe, Takashi Ohtsuki

    Bioconjugate chemistry   24 ( 10 )   1669 - 73   2013.10

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    DOI： 10.1021/bc4001195

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  • The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and nonsubstrate tRNA via an induced-fit process. Reviewed International journal

    Anna Ochi, Koki Makabe, Ryota Yamagami, Akira Hirata, Reiko Sakaguchi, Ya-Ming Hou, Kazunori Watanabe, Osamu Nureki, Kunihiro Kuwajima, Hiroyuki Hori

    The Journal of biological chemistry   288 ( 35 )   25562 - 25574   2013.8

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    DOI： 10.1074/jbc.M113.485128

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  • Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells. Reviewed International journal

    Kazunori Watanabe, Ryu Miyagawa, Chie Tomikawa, Rie Mizuno, Akihisa Takahashi, Hiroyuki Hori, Kenichi Ijiri

    Nucleic acids research   41 ( 8 )   4671 - 85   2013.4

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    DOI： 10.1093/nar/gkt153

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  • Formation of tRNA granules in the nucleus of heat-induced human cells. Reviewed International journal

    Ryu Miyagawa, Rie Mizuno, Kazunori Watanabe, Kenichi Ijiri

    Biochemical and biophysical research communications   418 ( 1 )   149 - 55   2012.2

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    DOI： 10.1016/j.bbrc.2011.12.150

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  • Molecular basis for maintenance of fidelity during the CCA-adding reaction by a CCA-adding enzyme. Reviewed International journal

    Yukimatsu Toh, Tomoyuki Numata, Kazunori Watanabe, Daijiro Takeshita, Osamu Nureki, Kozo Tomita

    The EMBO journal   27 ( 14 )   1944 - 52   2008.7

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    DOI： 10.1038/emboj.2008.124

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  • Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase. Reviewed International journal

    Kazunori Watanabe, Yukimatsu Toh, Kyoko Suto, Yoshihiro Shimizu, Natsuhisa Oka, Takeshi Wada, Kozo Tomita

    Nature   449 ( 7164 )   867 - 71   2007.10

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    DOI： 10.1038/nature06167

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  • Crystal structures of leucyl/phenylalanyl-tRNA-protein transferase and its complex with an aminoacyl-tRNA analog. Reviewed International journal

    Kyoko Suto, Yoshihiro Shimizu, Kazunori Watanabe, Takuya Ueda, Shuya Fukai, Osamu Nureki, Kozo Tomita

    The EMBO journal   25 ( 24 )   5942 - 50   2006.12

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    DOI： 10.1038/sj.emboj.7601433

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  • Functional categorization of the conserved basic amino acid residues in TrmH (tRNA (Gm18) methyltransferase) enzymes. Reviewed International journal

    Kazunori Watanabe, Osamu Nureki, Shuya Fukai, Yaeta Endo, Hiroyuki Hori

    The Journal of biological chemistry   281 ( 45 )   34630 - 9   2006.11

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    DOI： 10.1074/jbc.M606141200

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  • Roles of conserved amino acid sequence motifs in the SpoU (TrmH) RNA methyltransferase family. Reviewed International journal

    Kazunori Watanabe, Osamu Nureki, Shuya Fukai, Ryohei Ishii, Hironori Okamoto, Shigeyuki Yokoyama, Yaeta Endo, Hiroyuki Hori

    The Journal of biological chemistry   280 ( 11 )   10368 - 77   2005.3

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    DOI： 10.1074/jbc.M411209200

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  • Structural change of tRNA (Gm18) methyltransferase by binding of methyl donor analogues. Reviewed

    Watanabe K, Nureki O, Fukai S, Endo Y, Hori H

    Nucleic acids symposium series (2004)   ( 49 )   301 - 302   2005

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  • Substrate tRNA recognition mechanism of tRNA (m7G46) methyltransferase from Aquifex aeolicus. Reviewed International journal

    Hironori Okamoto, Kazunori Watanabe, Yoshiho Ikeuchi, Tsutomu Suzuki, Yaeta Endo, Hiroyuki Hori

    The Journal of biological chemistry   279 ( 47 )   49151 - 9   2004.11

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    DOI： 10.1074/jbc.M408209200

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  • Deep knot structure for construction of active site and cofactor binding site of tRNA modification enzyme. Reviewed International journal

    Osamu Nureki, Kazunori Watanabe, Shuya Fukai, Ryohei Ishii, Yaeta Endo, Hiroyuki Hori, Shigeyuki Yokoyama

    Structure (London, England : 1993)   12 ( 4 )   593 - 602   2004.4

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    DOI： 10.1016/j.str.2004.03.003

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  • Aquifex aeolicus tRNA (Gm18) methyltransferase has unique substrate specificity. TRNA recognition mechanism of the enzyme. Reviewed International journal

    Hiroyuki Hori, Susumu Kubota, Kazunori Watanabe, Jong-Myong Kim, Tomio Ogasawara, Tatsuya Sawasaki, Yaeta Endo

    The Journal of biological chemistry   278 ( 27 )   25081 - 90   2003.7

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    DOI： 10.1074/jbc.M212577200

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MISC

  • Light-responsive RNA delivery

    Kazunori Watanabe, Takashi Ohtsuki

    Drug Delivery System   37 ( 3 )   229 - 236   2022.7

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    Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)   Publisher：Japan Society of Drug Delivery System  

    DOI： 10.2745/dds.37.229

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  • Photoinduced Endosomal Escape Mechanism: A View from Photochemical Internalization Mediated by CPP-Photosensitizer Conjugates Reviewed International coauthorship

    Tet Htut Soe, Kazunori Watanabe, Takashi Ohtsuki

    Molecules   26 ( 1 )   36 - 36   2020.12

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    Endosomal escape in cell-penetrating peptide (CPP)-based drug/macromolecule delivery systems is frequently insufficient. The CPP-fused molecules tend to remain trapped inside endosomes and end up being degraded rather than delivered into the cytosol. One of the methods for endosomal escape of CPP-fused molecules is photochemical internalization (PCI), which is based on the use of light and a photosensitizer and relies on photoinduced endosomal membrane destabilization to release the cargo molecule. Currently, it remains unclear how this delivery strategy behaves after photostimulation. Recent findings, including our studies using CPP-cargo-photosensitizer conjugates, have shed light on the photoinduced endosomal escape mechanism. In this review, we discuss the structural design of CPP-photosensitizer and CPP-cargo-photosensitizer conjugates, and the PCI mechanism underlying their application.

    DOI： 10.3390/molecules26010036

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  • RNAデリバリー

    渡邉和則, 大槻高史

    核酸科学ハンドブック   524 - 527   2020.12

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  • Control of Bioreactios by using Expanded Translation Systems Including Unnatural Amino Acids

    渡邉和則, 大槻高史

    CSJ Current Review   ( 36 )   96 - 100   2020.4

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  • RNAキャリア・光応答的RNAデリバリー

    渡邉和則, 大槻高史

    バイオマテリアル学会誌   38 ( 2 )   2020

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  • 創薬を支える光技術-光とRNAを用いた遺伝子発現制御法 Invited

    渡邉和則, 大槻高史

    光アライアンス   6 ( 6 )   1 - 5   2018

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  • Formation Mechanism and Cellular Functions of Nuclear Stress Bodies Induced by Heat Stress Invited Reviewed

    Yuichi Miyoshi, Kazunori Watanabe

    Thermal medicine   34 ( 3 )   23 - 34   2018

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  • Photocontrolled Intracellular RNA Delivery Using Nanoparticles or Carrier-Photosensitizer Conjugates. Invited Reviewed International journal

    Kazunori Watanabe, Takashi Ohtsuki

    Progress in molecular biology and translational science   139   101 - 19   2016

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  • Intracellular Delivery of RNA via RNA-Binding Proteins or Peptides Invited Reviewed

    Kazunori Watanabe, Takashi Ohtsuki

    Intracellular Delivery II   403 - 416   2014

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    DOI： 10.1007/978-94-017-8896-0_19

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  • アミノアシルtRNAタンパク質転移酵素の分子機構の解明 Invited Reviewed

    渡邉和則, 董雪松, 富田耕造

    生化学   81 ( 4 )   294 - 298   2009

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  • Molecular basis for peptide-bond formation by an aminoacyl-tRNA protein transferase

    Kazunori Watanabe, Yukimatsu Toh, Kozo Tomita

    Seikagaku   81 ( 4 )   294 - 298   2009

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  • tRNAを使うリボソームによらないアミノ酸付加反応：Nエンドルールへの目印

    渡邉和則, 富田耕造

    蛋白質核酸酵素   53 ( 9 )   1152 - 1157   2008

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  • Identification of Essential Amino Acid Residues of tRNA (Gm18) Methyltransferase for Methyl-Transfer Activity Reviewed

    Nucleic Acids Res. Supplement   1   33 - 34   2001

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Presentations

  • 熱耐性と開始tRNA分解を介した翻訳抑制および細胞死との関係性の解明

    青木結子, 梅本裕介, 長房すずか, 高橋昭久, 井尻憲一, 大槻高史, 渡邉和則

    日本分子生物学会  2023.12 

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  • 熱ストレスに依存した開始tRNA分解に関与するXrn1, Xrn2活性化機構の解明

    渡邉和則, 岩城香菜子, 篠原里菜, 安田奈緒, 甲斐健太郎, 大槻高史

    日本分子生物学会  2023.12 

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  • 熱ストレス依存的に起こる核内ストレス顆粒形成に関与するカルシウムイオンシグナル伝達機構解明

    古谷優治, 的野恭平, 大槻高史, 渡邉和則

    日本分子生物学会  2023.12 

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  • BNCT 治療に向けた生分解性ミセル型ホウ素製剤の開発

    井上晴喜, Abdul Basith Fithroni, 渡邉和則, 松浦栄次, 大槻高史

    日本バイオマテリアル学会  2023.11 

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    Event date： 2023.11

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  • 腫瘍特異性の付加とアポトーシス誘導効率を向上させた光温熱剤の開発

    杉原桃香, 大槻高史, 渡邉和則

    日本バイオマテリアル学会  2023.11 

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    Event date： 2023.11

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  • 近赤外光に依存した高効率なアポトーシス誘導を可能とする光温熱剤の開発

    渡邉和則, 宮本麻衣, 松浦栄次, 大槻高史

    第47回日本分子生物学会  2024.12 

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  • 熱ストレス依存的なSAFB顆粒形成を誘導するCa2+-PAシグナル伝達機構解明

    古谷優治, 的野恭平, 高原茉莉, 大槻高史, 渡邉和則

    第47回日本分子生物学会  2024.12 

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  • 光化学的内在化による細胞質内mRNAデリバリー

    前本隼玖, 渡邉和則, 高原茉莉, 位髙啓史, 大槻高史

    第47回日本分子生物学会  2024.12 

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  • 哺乳動物初期胚への光による時空間特異的なRNA導入

    大槻高史, 井川優風, 若井拓哉, 舟橋弘晃, 渡邉和則

    第18回バイオ関連化学会  2024.9 

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  • ケージド相分離液滴の光応答挙動

    坂東晃成, 渡邉和則, 高原茉莉, 藤原誠一, 金崎佑紀, 北松瑞生, 大槻高史

    第18回バイオ関連化学会  2024.9 

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  • 酵素反応を用いた部位特異的脂質化抗体の合成とその応用

    高原茉莉, 渡邉和則, 大槻高史

    第18回バイオ関連化学会  2024.9 

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  • 近赤外光照射により高効率なアポトーシス誘導を行う光温熱剤の開発

    渡邉和則, 宮本麻衣, 松浦栄次, 大槻高史

    第41回日本ハイパーサーミア学会  2024.9 

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  • SAFB顆粒の形成には温熱依存的なホスファチジン酸濃度上昇が関与する

    古谷優治, 大槻高史, 渡邉和則

    第41回日本ハイパーサーミア学会  2024.9 

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  • Cell-penetrating peptide/photosensitizer conjugates for photo-triggered cytosolic delivery of RNAs and peptides

    Takashi Ohtsuki, Mizuki Kitamatsu, Kazunori Watanabe

    International Congress on Photobiology  2024.8 

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  • 磁気ハイパーサーミアのための腫瘍特異的磁性ナノ粒子

    周聖力, 今井律子, 三木裕紀子, 近藤杏菜, 中川大, 堤内要, 渡邉和則, 大槻高史

    第40回DDS学会  2024.7 

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  • Self-Assembly Nano Micelle of Alkylated Peptide Amphiphiles as Promising siRNA Delivery to Pancreatic Cancer Cells

    Taufik F.N. Hakim, Kazunori Watanabe, Shoumu Fujimoto, Mizuki Kitamatsu, Takashi Ohtsuki

    第40回DDS学会  2024.7 

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  • HSF1の液-液相分離機構と翻訳後修飾との関係性について

    渡邉和則, 小笠原悠人, 大槻高史

    日本ハイパーサーミア学会  2023.9 

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  • 温熱依存的に形成されるSAFB顆粒形成に関与するカルシウムイオンシグナル伝達機構解明

    古谷優治, 大槻高史, 渡邉和則

    日本ハイパーサーミア学会  2023.9 

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  • 腫瘍特異的ペプチドを用いた磁気温熱療法に向けた薬剤送達法の開発

    周聖力, 今井律子, 三木裕紀子, 近藤杏菜, 中川大, 河合憲康, 堤内要, 渡邉和則, 大槻高史

    日本ハイパーサーミア学会  2023.9 

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  • Development of a tumor-homing peptide-mediated drug delivery method for magnetic hyperthermia

    Shengli Zhou, Ritsuko Imai, Yukiko Miki, Anna Kondo, Hiroshi Nakagawa, Noriyasu Kawai, Kaname Tsutsumiuchi, Kazunori Watanabe, Takashi Ohtsuk

    Asian chemical biology conference  2023.8 

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  • Development and Characterization of Novel Peptide Amphiphiles for siRNA Delivery

    Taufik F.N. Hakim, Kazunori Watanabe, Mizuki Kitamatsu, Takashi Ohtsuki

    第七回日本核酸医薬学会  2023.7 

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  • 光増感剤と核酸キャリアの併用による光依存的mRNAデリバリー法

    前本隼玖, 渡邉和則, 位髙啓史, 大槻高史

    第45回日本光医学・光生物学会  2023.6 

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  • 温熱による開始tRNA分解と細胞周期依存的な翻訳抑制の関係性について

    竹本理恵, 梅本裕介, 長房すずか, 高橋昭久, 井尻憲一, 大槻高史, 渡邉和則

    日本分子生物学会  2022.12 

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  • HSF1、SASFB顆粒形成の抑制は温熱によるアポトーシスを増強する

    渡邉和則, 大槻高史

    日本分子生物学会  2022.12 

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  • PCI法の副作用を減らす試み

    坂東晃成, 渡邉和則, 大槻高史

    日本分子生物学会  2022.12 

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  • 哺乳動物における初期胚発生の光制御法の開発

    井川優風, 若井拓哉, 舟橋弘晃, 渡邉和則, 大槻高史

    日本バイオマテリアル学会  2022.11 

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  • 効率的なsiRNAの光依存的細胞内送達能を持つ生分解性ナノキャリアの開発

    田中七星, 渡邉和則, 小渕浩嗣, 久保貴紀, 松浦栄次, 大槻高史

    日本バイオマテリアル学会  2022.11 

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  • pre-miR-664a搭載ラクトソームを用いた光依存的なアポトーシス誘導法の開発

    宮本麻衣, 大槻高史, 松浦栄次, 渡邉和則

    バイオ関連化学シンポジウム  2022.9 

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  • 温熱依存的なSAFB顆粒形成はmTORC1が関与している

    的野 恭平, 井上 歩実, 岡田 真実, 山本 理紗子, 大槻 高史, 渡邉 和則

    日本分子生物学会  2021.12 

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  • miRNA検出のための新規PNA/DNAプローブの設計開発

    田原健太朗, 渡邉和則, 重藤元, 山村昌平, 岸高稚, 北松瑞生, 大槻高史

    日本分子生物学会  2021.12 

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  • pre-miR-664aとPCDR法を組み合わせた光依存的なアポトーシス誘導法

    渡邉和則, 縄稚朋子, 奥谷瑠璃子, 大槻高史

    日本分子生物学会  2021.12 

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  • RNA分解を検出する蛍光寿命プロープ

    平田陸, 平川和貴, 島田尚鷹, 渡邉和則, 大槻高史

    日本生化学会  2021.11 

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  • 細胞内還元環境下でCPPが離脱するCPP融合タンパク質の開発

    小林達哉, 中山真伍, 渡邉和則, 大槻高史

    日本化学会中四国支部会  2021.11 

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  • miRNA検出のための新規PNA/DNAプローブの設計開発

    田原健太朗, 大槻高史, 渡邊和則, 北松瑞生, 重藤元, 山村昌平, 岸高稚

    日本化学会中四国支部会  2021.11 

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    Presentation type：Oral presentation (general)  

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  • 細胞内への物質輸送のためPCI分子素子の開発

    角菜々子, 渡邉和則, 阿部由佳, 北松瑞生, 大槻高史

    日本生化学会  2021.11 

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    Presentation type：Poster presentation  

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  • 相分離を介した核内ストレス顆粒の形成は温熱抵抗性に関与している Invited

    渡邉和則, 的野恭平, 大槻高史

    日本ハイパーサーミア学会  2021.9 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

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  • Fluorophore-PNA-Quencher/Quencher-DNA probe for RNA detection

    Kentaro Tabara, Kazunori Watanabe, Hajime Shigeto, Shohei Yamamura, Takamasa Kishi, Mizuki Kitamatsu, Takashi Ohtsuki

    日本RNA学会  2021.7 

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    Presentation type：Poster presentation  

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  • Development of light-controlled apoptosis induction method Invited

    Kazunori Watanabe

    The 12th International symposium for future technology creating better human health and society  2021.3 

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    Presentation type：Symposium, workshop panel (nominated)  

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  • 核内ストレス顆粒の形成は温熱抵抗性に関与している

    渡邉和則, 大槻高史

    日本ハイパーサーミア学会  2020.9 

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    Presentation type：Oral presentation (general)  

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  • mTOR複合体を介した核内ストレス顆粒構成因子SAFB, Satellite Ⅲ RNA顆粒形成機構の解明

    的野 恭平, 井上 歩実, 岡田 真実, 山本 理紗子, 大槻 高史, 渡邉 和則

    日本ハイパーサーミア学会  2020.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • mTOR複合体制御による核内ストレス顆粒形成機構の解明

    渡邉和則, 井上歩実, 岡田真実, 山本理紗子, 大槻高史

    日本分子生物学会  2019.12 

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    Presentation type：Oral presentation (general)  

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  • mTOR複合体を介した核内ストレス顆粒形成機構の解明

    渡邉和則, 井上歩実, 岡田真実, 山本理紗子, 大槻高史

    日本ハイパーサーミア学会  2019.9 

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    Presentation type：Oral presentation (general)  

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  • 温熱による開始tRNA分解を介した翻訳抑制と細胞周期との関係性

    渡邉和則, 梅本裕介, 長房すずか, 高橋昭久, 井尻憲一, 大槻高史

    日本ハイパーサーミア学会  2018.8 

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    Presentation type：Oral presentation (general)  

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  • mTORを介した温熱による核内ストレス構造体形成機構

    渡邉和則, 岡田真実, 井上歩実, 山本理紗子, 大槻高史

    日本ハイパーサーミア学会  2017.9 

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  • 熱ストレスによる開始tRNAMetの細胞内動態

    渡邉和則, 宮川隆, 冨川千恵, 水野利恵, 高橋昭久, 大槻高史, 堀弘幸, 井尻憲一

    日本RNA学会  2013.7 

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    Presentation type：Oral presentation (general)  

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  • 温熱によるtRNA分解促進に対する耐性獲得機構の解明

    渡邉和則, 宮川隆, 水野利恵, 高橋昭久, 井尻憲一

    日本ハイパーサーミア学会  2011.9 

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  • ストレス誘導によるtRNA分解耐性機構の探索

    渡邉和則

    愛媛大学応用化学科セミナー  2011.7 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • アミノアシルtRNAタンパク質転移酵素によるペプチド結合形成機構の解明

    渡辺和則, 董雪松, 富田耕造

    RNA研究若手の会  2007.9 

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    Presentation type：Oral presentation (general)  

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  • アミノアシルtRNAタンパク質転移酵素によるペプチド結合反応触媒機構

    渡辺和則, 董雪松, 須藤恭子, 清水義弘, 岡夏央, 和田猛, 富田耕造

    日本RNA学会  2007.7 

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Awards

  • 生物学研究奨励賞

    2023.11   両備てい園記念財団  

    渡邉和則

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  • 研究助成 優秀賞

    2021.3   日本健康開発財団  

    渡邉 和則

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  • 第30回岡山工学振興会科学技術賞

    2018.7   公益財団法人岡山工学振興会  

    渡邉 和則

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  • 研究奨励賞

    2017.9   日本ハイパーサーミア学会  

    渡邉 和則

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  • 研究功績賞

    2017.3   岡山大学工学部  

    渡邉 和則

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  • 生物学研究奨励賞

    2014.10   両備てい園記念財団  

    渡邉 和則

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Research Projects

  • ケージド相分離ペプチドからなる相分離液滴の光制御と応用

    Grant number：25K01897  2025.04 - 2028.03

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    大槻 高史、渡邉和則、北松瑞生、高原茉莉

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    Authorship：Coinvestigator(s) 

    Grant amount：\18720000 （ Direct expense: \14400000 、 Indirect expense：\4320000 ）

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  • 第一および第二近赤外光に依存したRNA輸送による高効率アポトーシス誘導剤の開発

    2024.06 - 2025.03

    公益財団法人岡山工学振興会  第36回 一般研究助成 

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    Authorship：Principal investigator 

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  • 第一、第二近赤外光を用いた高効率アポトーシス誘導法の開発

    2024.06 - 2025.03

    公益財団法人ウエスコ学術振興財団  研究助成金 

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    Authorship：Principal investigator 

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  • SAFB顆粒形成阻害を標的とした新規作用機序を介した温熱増感剤の開発

    2024.04 - 2025.03

    公益財団法人 天野工業技術研究所  2024年度（前期募集）研究助成金 

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    Authorship：Principal investigator 

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  • 高効率なアポトーシス誘導を可能とする新規光温熱剤の開発

    2023.11 - 2025.03

    公益財団法人 テルモ生命科学振興財団  研究助成 

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  • がん細胞選択的光温熱剤の開発

    2023.11 - 2024.08

    公益財団法人両備檉園記念財団  研究助成 

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  • pre-miR-664aと腫瘍特異性を持ったペプチドを組み合わせることで高効率な細胞死誘導を可能とする光温熱剤の開発

    2023.05 - 2024.03

    公益財団法人ウエスコ学術振興財団  研究助成金 

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    Authorship：Principal investigator 

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  • 温熱抵抗性に関与するSAFB顆粒を標的にした新規温熱増感剤の開発

    2021.05 - 2022.03

    公益財団法人ウエスコ学術振興財団  研究助成金 

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    Authorship：Principal investigator 

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  • pre-miR-664a搭載ラクトソームによる光依存的なアポトーシス誘導法の開発

    Grant number：21K05277  2021.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    渡邉 和則

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    Authorship：Principal investigator 

    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

    本研究は、【アポトーシスを誘導するpre-miR-664aの標的mRNAの解明】、【pre-miR-664aとラクトソームを組み合わせた光依存的なアポトーシスの誘導法の開発】を目的に研究を進めている。2021年度は下記の課題を実施しました。
    (1) pre-miR-664aにより発現変動するmRNAの同定の試み
    マイクロアレイを用いてpre-miR-664aにより発現減少するmRNAをいくつか同定することができた。
    (2) 光依存的なアポトーシス誘導
    ラクトソームに細胞膜透過性ペプチド (CPP) を介してpre-miR-664aを搭載することに成功した。そこで、粒径測定やゼータ電位の測定を行なった。次に、光依存的にアポトーシスを誘導することができるか検証した。その結果、pre-miR-664aを搭載したラクトソームを用いることで光照射をしなければアポトーシスが誘導されず、光照射依存的にアポトーシスを誘導することができた。しかし、標的のないpre-miR-controlでも光依存的にアポトーシスが誘導されてしまった。そこで、ラクトソームに搭載している光増感剤を変更したpre-miR-664a搭載ラクトソームの調製に成功した。しかしながら、pre-miR-664a及びpre-miR-controlともに光依存的にアポトーシスが誘導されていた。このことから、光増感剤の力によりアポトーシスが誘導されていることが明らかになった。

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  • 核内ストレス顆粒形成を標的にした効果的な温熱による細胞死誘導薬剤の探索

    2020 - 2021

    一般財団法人 日本健康開発財団  研究助成 

    渡邉 和則

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  • miR-664a前駆体によるアポトーシス誘導機構の解明

    2019 - 2021

    公益財団法人 テルモ生命科学振興財団  研究助成 

    渡邉 和則

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  • マイクロRNA-664aを用いたアポトーシス制御法の開発とアポトーシス誘導機構の解明

    2018 - 2019

    岡山工学振興会  第30回岡山工学振興会科学技術賞 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • RNA分解酵素Xrn1によるRNA認識機構の解明

    2017 - 2020

    JSPS  科研費若手B 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • Xrn2による開始tRNA分解を介した翻訳抑制機構の解明

    2017 - 2019

    内藤記念科学振興財団  内藤記念科学奨励金・研究助成 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • マイクロRNAを用いた時空間制御可能なアポトーシス誘導法の開発

    2017 - 2018

    ノバルティス科学振興財団  ノバルティス研究奨励金 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • 時空間制御可能な神経分化制御法の開発

    2017 - 2018

    公益財団法人ウエスコ学術振興財団  研究助成金 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • The degradation of initiator tRNA depended on the cell cycle under heat stress

    2016

    倉田記念日立科学技術財団  海外渡航費補助金 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • 細胞内におけるRNA分解の観測法の開発とストレス応答研究への応用

    2015 - 2017

    JSPS  科研費 挑戦的萌芽 

    大槻 高史

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    Grant type：Competitive

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  • 光誘導RNA導入法を用いた細胞周期制御法の開発

    2015 - 2016

    中島記念国際交流財団  日本人若手研究者研究助成金 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • 光制御による細胞分化誘導法の開発

    2015 - 2016

    服部報公会  工学研究奨励援助金 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • PCDR法による２種RNAの時間差導入および細胞機能の制御

    2014 - 2017

    JSPS  科研費基盤研究B 

    大槻 高史

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    Grant type：Competitive

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  • 熱ストレス下での細胞周期依存性tRNA分解促進機構の解明

    2014 - 2016

    倉田記念日立科学技術財団  倉田奨励金 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • 熱ストレス下でのtRNA輸送機構の解明

    2014 - 2016

    JSPS  科研費若手B 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • マイクロRNAによる細胞分化制御法の開発

    2014 - 2015

    両備てい園記念財  研究奨励賞 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • mTOR regulates the degradation of initiator tRNAMet under heat stress

    2014

    倉田記念日立科学技術財団  海外渡航費補助金 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • Optical control of cellular functions by PCDR technology

    Grant number：25282232  2013.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Ohtsuki Takashi, WATANABE KAZUNORI

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    Grant amount：\18590000 （ Direct expense: \14300000 、 Indirect expense：\4290000 ）

    In this study, methods to control cellular functions by visible and infrared light were developed based on “Photoinduced Cytosolic Dispersion of RNA (PCDR) technology” for introducing RNAs into cytoplasm only within irradiated cells. Optimization of PCDR using infrared light, temporal control of introduction of two kinds of RNAs, the application to speroid (three-dimensional multicell model), and optical control of the cell functions have been archived.

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  • 熱ストレス誘導によるtRNA凝集体の形成機構の解明

    2012 - 2014

    内藤記念科学振興財団  内藤記念科学奨励金・研究助成 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • タンパク質分解経路における初期シグナル付加反応の分子機構の解明

    2009 - 2010

    JSPS  特別研究員奨励費 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • TrmHファミリーの生産物難解メカニズムの解明

    2005 - 2006

    理工学振興会  研究奨励賞 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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  • タンパク質構造と機能を元にしたSPOUT酵素群の機能進化についての研究

    2004 - 2005

    理工学振興会  研究奨励賞 

    渡邉 和則

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    Authorship：Principal investigator  Grant type：Competitive

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Class subject in charge

  • Advanced Internship for Interdisciplinary Medical Sciences and Engineering （2024academic year） Year-round  - その他

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2024academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2024academic year） Year-round  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2024academic year） Year-round  - その他

  • Molecular transport science （2024academic year） Prophase  - その他

  • Experiment for Chemistry and Biotechnology 2 （2024academic year） Fourth semester  - 月5～8

  • Synthetic Chemistry Experiment 1 （2024academic year） Fourth semester  - 月5～8

  • Introduction to Information Processing 2 （2024academic year） Second semester  - 月1～2

  • Introduction to Information Processing 2 （2024academic year） Second semester  - 木1～2

  • Material Process Experiment 1 （2024academic year） Fourth semester  - 月5～8

  • Biomolecular transport science （2024academic year） Late  - 金1～2

  • Biotechnology experiment 1 （2024academic year） Fourth semester  - 月5～8

  • Gene Engineering （2024academic year） Second semester  - 月1～2

  • Gene Engineering （2024academic year） Second semester  - 月1～2

  • Advanced Internship for Interdisciplinary Medical Sciences and Engineering （2023academic year） Year-round  - その他

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2023academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2023academic year） Year-round  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2023academic year） Year-round  - その他

  • Molecular transport science （2023academic year） Prophase  - その他

  • Experiment for Chemistry and Biotechnology 2 （2023academic year） Fourth semester  - 月5～8

  • Synthetic Chemistry Experiment 1 （2023academic year） Fourth semester  - 月5～8

  • Introduction to Information Processing 2 （2023academic year） Second semester  - 木1～2

  • Introduction to Information Processing 2 （2023academic year） Second semester  - 月1～2

  • Material Process Experiment 1 （2023academic year） Fourth semester  - 月5～8

  • Biotechnology experiment 1 （2023academic year） Fourth semester  - 月5～8

  • Gene Engineering （2023academic year） Second semester  - 月1～2

  • Gene Engineering （2023academic year） Second semester  - 月1～2

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2022academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2022academic year） Year-round  - その他

  • Experiment for Chemistry and Biotechnology 2 （2022academic year） Fourth semester  - 月5～8

  • Synthetic Chemistry Experiment 1 （2022academic year） Fourth semester  - 月5～8

  • Introduction to Information Processing 2 （2022academic year） Second semester  - 木1～2

  • Introduction to Information Processing 2 （2022academic year） Second semester  - 月1～2

  • Material Process Experiment 1 （2022academic year） Fourth semester  - 月5～8

  • Biotechnology experiment 1 （2022academic year） Fourth semester  - 月5～8

  • RNA technology （2022academic year） Prophase  - 不開講

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2021academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2021academic year） Year-round  - その他

  • Introduction to Information Processing 2 （2021academic year） Second semester  - 月1～2

  • Introduction to Information Processing 2 （2021academic year） Second semester  - 木1～2

  • Biotechnology experiment 1 （2021academic year） Fourth semester  - 月5,月6,月7,月8,木5,木6,木7,木8

  • Biotechnology experiment 1 （2021academic year） Fourth semester  - 月5,月6,月7,月8,木5,木6,木7,木8

  • RNA technology （2021academic year） Prophase  - 火5～6

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2020academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2020academic year） Year-round  - その他

  • General Exercises for Interdisciplinary Medical Sciences and Engineering （2020academic year） Late  - その他

  • Biotechnology experiment 1 （2020academic year） Fourth semester  - 月4,月5,月6,月7,木4,木5,木6,木7

  • Biotechnology experiment 1 （2020academic year） Fourth semester  - 月4,月5,月6,月7,木4,木5,木6,木7

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