Faculty Profiles - SAKAMOTO Hirotaka

MISC - SAKAMOTO Hirotaka

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小胞体内凝集体形成機序の解明-家族性中枢性尿崩症モデルマウスを用いた検討-

宮田崇, 萩原大輔, 萩原大輔, 橡谷昌佳, 森下啓明, 坂本浩隆, 有馬寛

日本内分泌学会雑誌 94 ( 1 ) 2018

マウスとラットを用いた痒み伝達機構の比較解析

高浪景子, 坂本浩隆, 小出剛

日本分子生物学会年会プログラム・要旨集(Web) 41st 2018

バソプレシンニューロンにおける異常蛋白の処理機構に小胞体シャペロンBiPおよびライソソームが関与する

宮田崇, 萩原大輔, 萩原大輔, 川口頌平, 栗本隼樹, 尾崎創, 光本一樹, 高木博史, 須賀英隆, 坂本浩隆, 有馬寛

バゾプレシン研究会プログラム・講演抄録 29th 2018

霊長類ニホンザルにおけるGastrin-releasing peptide神経系の機能局在

高浪景子, 伊藤隆志, 越智拓海, 小林靖尚, 佐藤慧太, 上田康雅, 坂本竜哉, 坂本浩隆

日本解剖学会総会・全国学術集会講演プログラム・抄録集 123rd 2018

Identification of the sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that controls male reproductive function in the mouse and Asian house musk shrew (Suncus murinus)

Kei Tamura, Yasuhisa Kobayashi, Asuka Hirooka, Keiko Takanami, Takumi Oti, Takamichi Jogahara, Sen-ichi Oda, Tatsuya Sakamoto, Hirotaka Sakamoto

JOURNAL OF COMPARATIVE NEUROLOGY 525 ( 7 ) 1586 - 1598 2017.5

Language：English Publisher：WILEY

Several regions of the brain and spinal cord control male reproductive function. We previously demonstrated that the gastrin-releasing peptide (GRP) system, located in the lumbosacral spinal cord of rats, controls spinal centers to promote penile reflexes during male copulatory behavior. However, little information exists on the male-specific spinal GRP system in animals other than rats. The objective of this study was to examine the functional generality of the spinal GRP system in mammals using the Asian house musk shrew (Suncus murinus; suncus named as the laboratory strain), a specialized placental mammal model. Mice are also used for a representative model of small laboratory animals. We first isolated complementary DNA encoding GRP in suncus. Phylogenetic analysis revealed that suncus preproGRP was clustered to an independent branch. Reverse transcription-PCR showed that GRP and its receptor mRNAs were both expressed in the lumbar spinal cord of suncus and mice. Immunohistochemistry for GRP demonstrated that the sexually dimorphic GRP system and male-specific expression/distribution patterns of GRP in the lumbosacral spinal cord in suncus are similar to those of mice. In suncus, we further found that most GRP-expressing neurons in males also express androgen receptors, suggesting that this male-dominant system in suncus is also androgen-dependent. Taken together, these results indicate that the sexually dimorphic spinal GRP system exists not only in mice but also in suncus, suggesting that this system is a conserved property in mammals. J. Comp. Neurol. 525:1586-1598, 2017. (c) 2016 Wiley Periodicals, Inc.

DOI： 10.1002/cne.24138

Web of Science

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蛍光タンパク質Venus導入ラットを用いた雄の性機能を司る脊髄神経回路系の解析

越智拓海, 高浪景子, 松田賢一, 河田光博, 坂本竜哉, 坂本浩隆

日本アンドロロジー学会総会記事 36th 2017

コペプチンからバソプレシン遺伝子の転写-翻訳-プロセシングを解析する試み

坂本浩隆, 坂本浩隆, 佐藤慧太, 高浪景子, 大坪秋人, 坂本竜哉, MORRIS John F.

日本神経内分泌学会学術集会プログラム・抄録集 44th 2017

行動を司る時間・空間的神経内分泌制御メカニズム

坂本浩隆

日本神経内分泌学会学術集会プログラム・抄録集 43rd 2016

雄の性機能を司る脊髄神経回路系の機能解析:Grp-promoter-Venusトランスジェニックラットの作出と利用

越智拓海, 高浪景子, 高浪景子, 松田賢一, 河田光博, 坂本竜哉, 坂本浩隆

日本神経内分泌学会学術集会プログラム・抄録集 43rd 2016

ニホンザル脊髄における痒み特異的伝達分子gastrin-releasing peptide受容体の発現

伊藤隆志, 高浪景子, 高浪景子, 越智拓海, 小林靖尚, 小林靖尚, 佐藤慧太, 高橋俊次, 上田康雅, 坂本竜哉, 坂本浩隆

日本組織細胞化学会総会・学術集会講演プログラム・予稿集 57th 2016

「基礎系」オキシトシンと性機能

坂本浩隆

日本性機能学会雑誌 31 ( 2 ) 2016

Requirement of vasotocin for being a winner in love triangle relationships (two males and one female) of medaka fish Invited

Saori Yokoi, Tatsuya Sakamoto, Hirotaka Sakamoto, Hideaki Takeuchi

37 ( 6 ) 591 - 597 2015.12

超高圧電子顕微鏡・トモグラフィー法を用いた脊髄内痒み神経ネットワークの解析

坂本浩隆, 佐藤慧太, 高浪景子, 高浪景子, 高浪景子, 村田和義, 河田光博, 坂本竜哉

日本内分泌学会雑誌 91 ( 2 ) 525 - 525 2015.9

In vivo imaging of axonal transport of mitochondria in the diseased and aged mammalian CNS

Yuji Takihara, Masaru Inatani, Kei Eto, Toshihiro Inoue, Alexander Kreymerman, Seiji Miyake, Shinji Ueno, Masatoshi Nagaya, Ayami Nakanishi, Keiichiro Iwao, Yoshihiro Takamura, Hirotaka Sakamoto, Keita Satoh, Mineo Kondo, Tatsuya Sakamoto, Jeffrey L. Goldberg, Junichi Nabekura, Hidenobu Tanihara

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 112 ( 33 ) 10515 - 10520 2015.8

Language：English Publisher：NATL ACAD SCIENCES

The lack of intravital imaging of axonal transport of mitochondria in the mammalian CNS precludes characterization of the dynamics of axonal transport of mitochondria in the diseased and aged mammalian CNS. Glaucoma, the most common neurodegenerative eye disease, is characterized by axon degeneration and the death of retinal ganglion cells (RGCs) and by an age-related increase in incidence. RGC death is hypothesized to result from disturbances in axonal transport and in mitochondrial function. Here we report minimally invasive intravital multiphoton imaging of anesthetized mouse RGCs through the sclera that provides sequential time-lapse images of mitochondria transported in a single axon with submicrometer resolution. Unlike findings from explants, we show that the axonal transport of mitochondria is highly dynamic in the mammalian CNS in vivo under physiological conditions. Furthermore, in the early stage of glaucoma modeled in adult (4-mo-old) mice, the number of transported mitochondria decreases before RGC death, although transport does not shorten. However, with increasing age up to 23-25 mo, mitochondrial transport (duration, distance, and duty cycle) shortens. In axons, mitochondria-free regions increase and lengths of transported mitochondria decrease with aging, although totally organized transport patterns are preserved in old (23- to 25-mo-old) mice. Moreover, axonal transport of mitochondria is more vulnerable to glaucomatous insults in old mice than in adult mice. These mitochondrial changes with aging may underlie the age-related increase in glaucoma incidence. Our method is useful for characterizing the dynamics of axonal transport of mitochondria and may be applied to other submicrometer structures in the diseased and aged mammalian CNS in vivo.

DOI： 10.1073/pnas.1509879112

Web of Science

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P4-10 医学・生物学分野における超高圧電子顕微鏡・トモグラフィー法の再考 : A revisiting study for 3D-EM(細胞生物・電子顕微鏡,ポスター発表,組織化学のモーダルシフト,第56回日本組織細胞化学会総会・学術集会)

坂本浩隆, 佐藤慧太, 高浪景子, 村田和義, 河田光博, 坂本竜哉

日本組織細胞化学会総会プログラムおよび抄録集 ( 56 ) 71 - 71 2015