Faculty Profiles - SAKAMOTO Hirotaka

MISC - SAKAMOTO Hirotaka

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オキシトシン放出を調節する1回膜貫通型タンパク質CD38のはたらき

坂本浩隆, 越智拓海, 越智拓海, 大坪秋人, 川上奈津子, 坂本竜哉

日本解剖学会総会・全国学術集会講演プログラム・抄録集 127th (CD-ROM) 2022

ガストリン放出ペプチド神経系による体性感覚調節機構

高浪景子, 坂本浩隆

日本解剖学会総会・全国学術集会講演プログラム・抄録集 127th (CD-ROM) 2022

オキシトシンによる脊髄射精中枢を介した男性性機能の調節メカニズム

越智拓海, 越智拓海, 坂本竜哉, 坂本浩隆

日本性機能学会中部総会プログラム・抄録集 32nd 2022

バソプレシンニューロンにおいて異常タンパク凝集体は小胞体から輸送隔離されることなく小胞体内部で分解される-家族性中枢性尿崩症モデルマウスを用いた検討-

宮田崇, 萩原大輔, 津村哲郎, 蓬臺優一, 川口頌平, 栗本隼樹, 高木博史, 須賀英隆, 川上奈津子, 坂本浩隆, 松本真実, 大野伸彦, 大野伸彦, 有馬寛

日本内分泌学会雑誌 97 ( 1 ) 2021

内側視索前野の性的二型核に発現するガストリン放出ペプチド系は雄ラットの性的活性を制御する

前嶋翔, 野村黎, 高浪景子, 坂本竜哉, 坂本浩隆

日本神経内分泌学会学術集会プログラム・抄録集 47th 2021

17β-エストラジオールによる痒み閾値調節

高浪景子, 歌大介, 松田賢一, 河田光博, 河田光博, CARSTENS Earl, 坂本竜哉, 坂本浩隆

日本神経内分泌学会学術集会プログラム・抄録集 47th 2021

コペプチン動態に着目したバソプレシン前駆体・プロセシング機構の解明

大坪秋人, 川上奈津子, 前嶋翔, 坂本竜哉, 坂本浩隆

バゾプレシン研究会プログラム・講演抄録 31st 2021

Localization of Oxytocin and Oxytocin Receptors in the Rat Testis

ZUN Miho, 伊藤隆志, 坂本竜哉, 小谷享, 坂本浩隆, 越智拓海, 越智拓海

Science Journal of Kanagawa University 32 2021

痒みの神経伝達基盤-疾患モデル動物を用いた検討-

高浪景子, 小出剛, 坂本浩隆

日本内分泌学会雑誌 97 ( 1 ) 2021

性経験のないオスメダカは初めての性的パートナーに対して配偶者選好性を示す

大門将寛, 安齋賢, 勝村啓史, 坂本浩隆, 竹内秀明

日本動物行動学会大会発表要旨集 40th (CD-ROM) 2021

ミネラルコルチコイド受容体ノックアウト(MR-KO)メダカにおける異常な視覚依存行動の観察

唐嘉榮, 今野紀文, 中町智哉, 坂本浩隆, 坂本竜哉, 松田恒平

日本比較内分泌学会大会及びシンポジウムプログラム・講演要旨 45th 2021

扁形動物ヒラムシから紐解くGnRHの祖先型機能

森俊輔, 酒井丈実, 濱田麻友子, 坂本竜哉, 坂本浩隆

日本比較内分泌学会大会及びシンポジウムプログラム・講演要旨 45th 2021

雄ラット扁桃体内側核後背側部におけるガストリン放出ペプチド系を介した性行動調節メカニズムの解明

大坪秋人, 上田涼太, 高松廉, 大野智輝, 前嶋翔, 越智拓海, 越智拓海, 犬束歩, 尾仲達史, 坂本竜哉, 坂本浩隆

日本神経内分泌学会学術集会プログラム・抄録集 47th 2021

雄ラット視床下部腹内側核オキシトシン受容体ニューロン系による食欲と性欲のスイッチング機構

立石沙也加, 上田涼太, 永渕詢大, 越智拓海, 越智拓海, 犬束歩, 尾仲達史, VALERY Grinevich, 坂本竜哉, 坂本浩隆

日本神経内分泌学会学術集会プログラム・抄録集 47th 2021

'Central' Actions of Corticosteroid Signaling Suggested by Constitutive Knockout of Corticosteroid Receptors in Small Fish. International journal

Tatsuya Sakamoto, Hirotaka Sakamoto

Nutrients 11 ( 3 ) 2019.3

Language：English

This review highlights recent studies of the functional implications of corticosteroids in some important behaviors of model fish, which are also relevant to human nutrition homeostasis. The primary actions of corticosteroids are mediated by glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), which are transcription factors. Zebrafish and medaka models of GR- and MR-knockout are the first constitutive corticosteroid receptor-knockout animals that are viable in adulthood. Similar receptor knockouts in mice are lethal. In this review, we describe the physiological and behavioral changes following disruption of the corticosteroid receptors in these models. The GR null model has peripheral changes in nutrition metabolism that do not occur in a mutant harboring a point mutation in the GR DNA-binding domain. This suggests that these are not "intrinsic" activities of GR. On the other hand, we propose that integration of visual responses and brain behavior by corticosteroid receptors is a possible "intrinsic"/principal function potentially conserved in vertebrates.

DOI： 10.3390/nu11030611

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雄ラットにおける性行動への内側視索前野の性的二型核の関与

前嶋翔, 野村黎, 高浪景子, 高浪景子, 坂本竜哉, 坂本浩隆

日本比較内分泌学会大会及びシンポジウムプログラム・講演要旨 44th 2019

魚類をモデルとしたアンドロゲンによる二次性徴形質の発現と多様化の分子機構

荻野由紀子, 安齋賢, 坂本浩隆, 渡辺英治, 成瀬清, 井口泰泉

日本分子生物学会年会プログラム・要旨集(Web) 42nd 2019

Early-life exposure to Tris(1,3-dichloroisopropyl) phosphate induces dose-dependent suppression of sexual behavior in male rats. International journal

Manami Kamishima, Tatsuya Hattori, Go Suzuki, Hidenori Matsukami, Chiaki Komine, Yasuyuki Horii, Gen Watanabe, Takumi Oti, Hirotaka Sakamoto, Tomoko Soga, Ishwar S Parhar, Yasuhiko Kondo, Hidetaka Takigami, Maiko Kawaguchi

Journal of applied toxicology : JAT 38 ( 5 ) 649 - 655 2018.5

Language：English

Exposure to endocrine-disrupting chemicals may adversely affect animals, particularly during development. Tris(1,3-dichloroisopropyl) phosphate (TDCIPP) is an organophosphate with anti-androgen function in vitro that is present in indoor dust at relatively high concentrations. In male rats, androgens are necessary for the development of reproductive organs, as well as the endocrine and central nervous systems. However, we currently do not know the exact effects of TDCIPP exposure through suckling on subsequent reproductive behavior in males. Here, we show that TDCIPP exposure (25-250 mg kg-1 via oral administration over 28 consecutive days post-birth) suppressed male sexual behavior and reduced testes size. These changes were dose-dependent and appeared first in adults rather than in juveniles. These results demonstrate that TDCIPP exposure led to normal body growth and appearance in juveniles, but disrupted the endocrine system and physiology in adults. Therefore, assays should be performed using adult animals to ensure accuracy, and to confirm the influence of chemical substances given during early mammalian life.

DOI： 10.1002/jat.3569

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Other Link： http://orcid.org/0000-0002-6224-4591

Analyzing the effects of co-expression of chick (Gallus gallus) melanocortin receptors with either chick MRAP1 or MRAP2 in CHO cells on sensitivity to ACTH(1–24) or ACTH(1–13)NH2: Implications for the avian HPA axis and avian melanocortin circuits in the hypothalamus

Alexa L. Thomas, Fumihiko Maekawa, Takaharu Kawashima, Hirotaka Sakamoto, Tatsuya Sakamoto, Perry Davis, Robert M. Dores

General and Comparative Endocrinology 256 50 - 56 2018.1

Language：English Publisher：Academic Press Inc.

In order to better understand the roles that melanocortin receptors (cMCRs) and melanocortin-2 receptor accessory proteins (cMRAP1 and cMRAP2) play in the HPA axis and hypothalamus, adrenal gland and hypothalamus mRNA from 1 day-old white leghorn chicks (Gallus gallus), were analyzed by real-time PCR. mRNA was also made for kidney, ovary, and liver. Mrap1 mRNA could be detected in adrenal tissue, but not in any of the other tissues, and mrap2 mRNA was also detected in the adrenal gland. Finally, all five melanocortin receptors mRNAs could be detected in the adrenal gland
mc2r and mc5r mRNAs were the most abundant. To evaluate any potential interactions between MRAP1 and the MCRs that may occur in adrenal cells, individual chick mcr cDNA constructs were transiently expressed in CHO cells either in the presence or absence of a chick mrap1 cDNA, and the transfected cells were stimulated with hACTH(1–24) at concentrations ranging from 10−13 M to 10−6 M. As expected, MC2R required co-expression with MRAP1 for functional expression
whereas, co-expression of cMC3R with cMRAP1 had no statistically significant effect on sensitivity to hACTH(1–24). However, co-expression of MC4R and MC5R with MRAP1, increased sensitivity for ACTH(1–24) by approximately 35 fold and 365 fold, respectively. However, co-expressing of cMRAP2 with these melanocortin receptors had no effect on sensitivity to hACTH(1–24). Since the real-time PCR analysis detected mrap2 mRNA and mc4r mRNA in the hypothalamus, the interaction between cMC4R and cMRAP2 with respect to sensitivity to ACTH(1–13)NH2 stimulation was also evaluated. However, no effect, either positive or negative, was observed. Finally, the highest levels of mc5r mRNA were detected in liver cells. This observation raises the possibility that in one-day old chicks, activation of the HPA axis may also involve a physiological response from liver cells.

DOI： 10.1016/j.ygcen.2017.09.002

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ラット内側視索前野において雄の性行動を司る神経回路系の解析

野村黎, 高浪景子, 高浪景子, 坂本竜哉, 坂本浩隆

日本神経内分泌学会学術集会プログラム・抄録集 45th 2018

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