研究者詳細 - 中野　敬介

MISC - 中野　敬介

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Detection of mRNA in hard tissue using in situ hybridization

Ishiwari,Y, Nagatsuka, H, Tsujigiwa, H, Nakano, K, Nagai, N

PROCEEDINGS of Asian Science Seminar in Okayama 2000 2000年

Immunohistochemistry in hard tissue research

Nagatsuka, H, Ishiwari,Y, Nakano, K, Tsujigiwa, H

PROCEEDINGS of Asian Science Seminar in Okayama 2000 2000年

14. Distribution pattern of αl(IV)- α6(IV)chains of type IV collagen in tooth germ morphogenesis(8th Annual Meeting of the Society of Hard Tissue Biology July 24, 1999 in Asahikawa)

Noriyuki Nagai, Keisuke Nakano, Yuzo Ishiwari, Hitoshi Nagatsuka, Yoshiko Kitamura, Tohru Takagi, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Biochemistry Faculty of Dentistry of Tokyo Medical and Dental University

Journal of hard tissue biology = Journal of hard tissue biology 9 ( 1 ) 27 - 28 2000年

IV型コラーゲンα3鎖欠損マウスの歯胚形成におけるα鎖の消長

井上正久, 中野敬介, 藤井英貴, 玉村亮, 永井教之

歯科基礎医学会雑誌 41 ( 5 ) 404 - 404 1999年8月

15. Natural bovine bone morphogenetic protein (BMP) induces cytological and functional differentiation of preodontoblasts in vitro. :

Murata Masaru, Tsujigiwa Hidetsugu, Liu Gui-ru, Nakano Keisuke, Akagi Takumi, Ruch Jean Victor, Nagai Noriyuki

日本硬組織研究技術学会雑誌 = Journal of hard tissue biology 7 ( 2 ) 68 - 68 1998年

記述言語：英語出版者・発行元：日本硬組織研究技術学会

First lower molar dental papillae, isolated from day-17 mouse embryos, were embedded in 10 E l of BGJ-b medium including fetal calf serum and 0.5% agar. Two E1 of BMP solution at the different dose of BMP were added to the medium before getting. The papillae embedded in the semi-solidmedium were deposited on Millipore filters, and cultured for 6 days. This BMP fraction promoted functional differentiatioun of odontoblast-like cells and stimtulated the matrix secretion, dependent on the concentration of BMP.

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=306655

12. Gene expressions of bone and cartilage matrix proteins during ectopic hard tissue formation induced by BMP. :

Takagi Hiroshi, Ishiwari Yuzo, Tsujigiwa Hidetsugu, Tan Jun, Nakano Keisuke, Nosaka Yukio, Nagathuka Hitoshi, Nagai Noriyuki

日本硬組織研究技術学会雑誌 = Journal of hard tissue biology 7 ( 2 ) 72 - 72 1998年

記述言語：英語出版者・発行元：日本硬組織研究技術学会

Gene expressions of bone and cartilage matrix protein during the ectopic hard tissue formation induced by BMP were investigated using in situ hybridization method. The gene expressions of type I and II as well as type X and osteocalcin, which is not able to be seen in normal chondral ossification, were observed in chondrocyte-like cells. The results indicate that chondrocyte-like cells in ectopic bone formation may have both characters of chondrocytes and osteoblasts.

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=306672

4. Gene expression of amelogenin in odontogenic tumors :

Nakano Keisuke, Nagatsuka Hitoshi, Inoue Masahisa, Takagi Tohru, Nagai Noriyuki

日本硬組織研究技術学会雑誌 = Journal of hard tissue biology 7 ( 2 ) 62 - 62 1998年

記述言語：英語出版者・発行元：日本硬組織研究技術学会

The distribtution of amelogenin mRNA was investigated in ameloblastoma and adenomatoid odontogeznic tumor (AOT). Digoxygenin(DIG) labeled RNA probe was prepared from human plexiform type amelobastoma. Sections of rat incisors human ameloblastoma and ACT were hybridized with the above-mentioned probe, and immuno-detection of the hybridized probe was performed with the DIG Ntucleic Acid Detection Kit. In the rat incisor, amelogenin mRNA was detected in the preameloblast and ameloblast. Amelogeuuin mRNA was detected mainly in the high columutar cells of the amelobastoma. Amelogenin mRNA was also detected in many duct-like and solid strtuctture tumor cells of AOT.

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=306625

13. Bone Augmentation by BMP-Collagen composite in calvarial subperiosteum of matured rats. :

Huang Bingzhen, Inoue Masahisa, Nakano Keisuke, Hoh Chouhuku, Nojima Tetsundo, Murata Masaru, Tsigono Yoshiho, Nagai Noriyuki

日本硬組織研究技術学会雑誌 = Journal of hard tissue biology 7 ( 2 ) 72 - 72 1998年

記述言語：英語出版者・発行元：日本硬組織研究技術学会

The composite of recombinant bone morphogenetic protein 2 (rhBMP-2) and type I atelocollagen fibers was implanted under the calvarial periosteum of wistar rats of 8 months old. Each composite was removed at 2, 4, 6, and 8 weeks after implantation, and evaluated histologically as an onlay bone graft substitute in a calvarial model. The results demonstrated that the composite of BMP and collagen was an effective material with osteoindtnctivity as an onlay bone graft substitute even in matured rats.

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=306673

Aging and Ectopic Bone Formation Induced by Partially Purified Bone Morphogenetic Protein : Blood Vessel Ingrowth and Localization of Type I Collagen and Osteocalcin Assessed by Immunohistochemistry

Chun-Lin QIN, Keisuke NAKANO, Yuzo ISHIWARI, Kazuteru HIBI, Katsuhiro KURODA, Yukio NOSAKA, Nobuyuki IIDA, Noriyuki NAGAI, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University

Journal of hard tissue biology 6 ( 3 ) 95 - 104 1997年10月

記述言語：英語

A mixture of heparin-Sepharose-purified bovine bone morphogenetic protein (BMP) and type I atellocollagen as the carrier was implanted in the subcutaneous tissues of 4-week, 10-month and 18-month old rats. The explants were evaluated usilrg H&E staining, morphometric analysis, as well as immunohistochemical staining of type IV collagen for blood vessel distribution, type I collagen and ostcocalcin for bone matrix proteins. Ectopic bone formation was reduced and the rate of blood vessel ingrowth was delayed and hindered in aged rats. The difference in blood vessel distribution might be one reason for the reductlon in ectopic bone formation in aged rats. The localization of type I collagen and osteocalcin was consistent with the bone formation observed histologically, L e., more extensive areas positive to type I collagen and osteocalcin in 4-week old rats than in 10-month and 18-month old rats.

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その他リンク： http://search.jamas.or.jp/link/ui/1998111752

Immunohistochemical Study of Cytokeratin Patterns in Follicular and Plexiform Ameloblastoma

Hitoshi NAGATSUKA, Hong-In SHIN, Hee-Kyung PARK, Yuzo ISHIWARI, Katsuhiro KURODA, Yukio NOSAKA, Han SONG, Chun-Lin QIN, Shao-Quan ZHANG, Keisuke NAKANO, Yoshiho CHIGONO, Hidetsugu TSUJIGIWA, Tohru TAKAGI, Noriyuki NAGAI, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Kyungpook National University, Department of Oral Pathology School of Dentistry Kyungpook National University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, First Department of Oral Surgery Faculty of Medicine Teikyo University, Department of Oral Pathology School of Dentistry Okayama University, Department of Biochemistry Tokyo Medical and Dental University Faculty of Dentistry Yushima, Department of Oral Pathology School of Dentistry Okayama University

Journal of hard tissue biology 4 ( 2 ) 50 - 56 1995年11月

記述言語：英語

The distribution of cytokeratin was investigated in follicular and plexiform ameloblastomas using immunohistochemical methods. The findings were compared with that of human fetal oral mucosa, developing tooth germ and adult gingiva. The following cytokeratin antibodies were used:CK-1,SE-K,NSE-K and 19-K. In the follicular ameloblastoma, the cytokeratin pattern of nest's structure was not comparable to that of the enamel organ at the bell stage. The peripheral columnar cells reacted positively for NES-K and 19-K and negatively for SE-K. The central stellate reticulum-like cells showed an immunoreactivity with all used markers of cytokeratins, as well as the outer enamel epithelium. In the plexiform ameloblastoma, the immunoreaction pattern was like that of the oral mucosal epithelium of the fetus. Both the central and peripheral cells demonstrated positive reactions with SE-K and 19-K but not with NSE-K. In the developing teeth, SE-K, NES-K and 1999-K were well demonstrated in the dental lamina, and each layer of the enamel organ. In the adult gingiva, contrary to the fetal oral mucosa, the basal cells did not show an immunoreactivity with 19-K except for Merkel cells. A clear difference in cytokeratin pattern was noted between ameloblastoma and tooth germs. Especially, the reactive pattern of NSE-K and 19-K showed differences between the peripheral columnar cells of ameloblastoma and the inner enamel epithelium. These findings support the concept that the columnar cells of follicular ameloblastomas have little resemblance to the pre-ameloblast like cells in the cytokeratin distribution.

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Gene Expression of Bone Matrix Protein mRNA during BMP Induced Chondrogenesis and Osteogenesis by in situ Hybridization

Noriyuki NAGAI, Hitoshi NAGATSUKA, Masaru MURATA, Masahisa INOUE, Takumi AKAGI, Chun-Lin QIN, Keisuke NAKANO, Yuzo ISIWARI, Hironobu KONOUCHI, Hidetsugu TSUJIGIWA, Yoshiho CHIGONO, Tohru TAKAGI, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Surgery Faculty of Medicine Teikyo University, Department of Biochemistry Tokyo Medical and Dental University Faculty of Dentistry

Journal of hard tissue biology 4 ( 1 ) 15 - 23 1995年3月

記述言語：英語

A sensitive in situ hybridization method with high resolution has been developed usin digoxigenin-II VTP labeled cRNA as a probe. The system was applied to a heterotopic induced bone with crude BMP. The localization of mRNA of the bone matrix proteins, osteopontin (OSP) and osteocalcin (OSC), was examined in the BMP-induced cartilage and bone in comparison with the endochondral ossification. In the heterotopic bone, OSP mRNA were detected in the osteoblast, young osteocyte and chondrocyte-like cells. In fatal endochondral ossification OSP mRNA was detected in the hypertrophic chondrocyte, osteoblast and young osteocyte, while OSC mRNA was detected in the osteoblast and young osteocyte, but hypertrophic chondrocyte was not detected. These results suggest that the chondrocyte-like cells in the BMP induced bone demonstrate the ambifunctions of the chondrocyte and osteoblast. Our results indicate that in situ hybridization immunohistochemistry is useful in identifying the cell phenotypes and their function.

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その他リンク： http://search.jamas.or.jp/link/ui/1997001332

顎骨のセメント質-骨形成性線維腫と化骨性線維腫の発生母組織に関する一考察

井上正久, 秦春林, 中野敬介, 辻極秀次, 此内浩信, 永井教之

Journal of hard tissue biology = 日本硬組織研究技術学会雑誌 4 ( 3 ) 9 - 14 1995年

記述言語：日本語出版者・発行元：日本硬組織研究技術学会

The histological differential diagnosis between cemento-ossifying fibroma and ossifying fibroma is still controversial since distingushing bone from cementum and non-specific calcification tissue by routine methods shows little reliabi11ty. Here we studied one case of each lesion by CPC treatment, immunohistochemical detection of collagen type I and III, and electron microscopy. We round that the distinction among bone, cementum and non-specific calcirication tissues might be greatly improved by these means, leading, therefore,to a more accurate differential diagnosis between cemento-ossirying and ossifying fibroma. The tissue origin in both cases is also discussed.

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=306557

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