2021/04/19 更新

写真a

アンドウ モトノリ
安藤 元紀
ANDO Motonori
所属
教育学域 教授
職名
教授
外部リンク

学位

  • 博士(医学)

研究キーワード

  • 自然科学教育

  • 聴覚

  • 細胞骨格

  • Cell physiology

  • 細胞運動

  • 循環

  • 膜輸送

  • 環境応答

研究分野

  • ライフサイエンス / 医療薬学

  • ライフサイエンス / 解剖学

  • ライフサイエンス / 動物生理化学、生理学、行動学

  • ライフサイエンス / 生理学

  • ライフサイエンス / 生理学

  • ライフサイエンス / 耳鼻咽喉科学

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経歴

  • 岡山大学大学院   教育学研究科   教授

    2012年 - 現在

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  • - Professor,Graduate School of Education,Okayama University

    2012年

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  • 岡山大学大学院   教育学研究科   准教授

    2005年 - 2012年

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  • Associate Professor,Graduate School of Education,Okayama University

    2005年 - 2012年

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  • Assistant Professor,Kochi Medical School

    1990年 - 2005年

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  • 高知大学   医学部   助教

    1990年 - 2005年

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所属学協会

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論文

  • Structural analysis of the statocyst and nervous system of <i>Praesagittifera naikaiensis</i>, an acoel flatworm, during development after hatching 査読

    Tosuke Sakagami, Kaho Watanabe, Risa Ikeda, Motonori Ando

    Zoomorphology   2021年4月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s00435-021-00521-9

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    その他リンク: http://link.springer.com/article/10.1007/s00435-021-00521-9/fulltext.html

  • Immunocytochemical analysis of α-tubulin distribution before and after rapid axopodial contraction in the centrohelid <i>Raphidocystis contractilis</i> 査読

    Risa Ikeda, Miki Kurokawa, Momoka Murai, Noboru Saito, Motonori Ando

    Acta Protozoologica59 ( 1 ) 1 - 12   2020年4月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Uniwersytet Jagiellonski - Wydawnictwo Uniwersytetu Jagiellonskiego  

    DOI: 10.4467/16890027ap.20.001.12157

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  • Differential localizations of the myo-inositol transporters HMIT and SMIT1 in the cochlear stria vascularis 査読

    Midori Edamatsu, Yasuhiro Kondo, Motonori Ando

    Neuroscience Letters674   88 - 93   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Ireland Ltd  

    The cochlear stria vascularis produces endolymph and thereby plays an active role in inner ear homeostasis. We recently reported that the H+/myo-inositol cotransporter (HMIT) gene is expressed in the stria vascularis. Here, we examined the protein localization of HMIT and Na+/myo-inositol cotransporter 1 (SMIT1) in the stria vascularis by immunohistochemistry. HMIT and SMIT1 were detected in the lateral wall of the cochlear duct. HMIT was widely detected throughout the stria vascularis, while SMIT1 was enriched in the strial basal cells. To examine the localization of HMIT in the stria vascularis in more detail, dissociated strial cells were immunostained, which resulted in the detection of HMIT immunoreactivity in marginal cells. These results indicate that HMIT is expressed in marginal cells and basal cells of the stria vascularis, while SMIT1 expression is enriched in basal cells. We speculate that HMIT and SMIT1 may play important roles in the homeostasis of cochlear fluids, for example by participating in pH regulation and osmoregulation.

    DOI: 10.1016/j.neulet.2018.03.028

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  • Cytoskeletal elements in an acoelomorph worm, <i>Praesagittifera naikaiensis</i>.

    Ikeda R, Fujiwara C, Hamada M, Sakamoto T, Saito N, Ando M

    Proceedings of Okayama Association for Laboratory Animal Science34   21 - 27   2018年

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    担当区分:責任著者  

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  • Structure and function of tegmentum vasculosum in avian cochlea.

    Ikeda R, Otono T, Ikeda N, Saito N, Ando M

    Proceedings of Okayama Association for Laboratory Animal Science33   26 - 30   2017年

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    担当区分:責任著者  

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  • Direct evidence of the glucose uptake into cochlear strial marginal cells: Application of a fluorescent tracer method combined with immunohistochemistry. 査読

    Hishikawa S, Edamatsu M, Inoue-Ikeda R, Ando M

    Bioimages23   1 - 8   2016年

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    担当区分:責任著者  

    DOI: 10.11169/bioimages.23.1

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  • Acetylcholine Suppresses Ventricular Arrhythmias and Improves Conduction and Connexin-43 Properties During Myocardial Ischemia in Isolated Rabbit Hearts 査読

    Takeshi Aiba, Takashi Noda, Ichiro Hidaka, Masashi Inagaki, Rajesh G. Katare, Motonori Ando, Kenji Sunagawa, Takayuki Sato, Masaru Sugimachi

    JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY26 ( 6 ) 678 - 685   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    ACh Prevents Ischemic Loss of Gj and Arrhythmias
    IntroductionAcetylcholine (ACh), a vagal efferent neurotransmitter, markedly improves survival in rats with myocardial ischemia (MI) by preventing ischemic loss of gap junction (Gj) and by inducing anti-apoptotic cascades. However, electrophysiological mechanisms of the antiarrhythmic effect of ACh after acute MI are still unclear.
    MethodsAcute MI was induced by ligation of the left anterior descending (LAD) coronary artery in Langendorff-perfused rabbit hearts with (ACh(+):n = 11) or without (ACh(-):n = 12) 10 mol/L ACh delivered continuously starting at 5 minutes before LAD ligation. Action potentials on the left ventricular (LV) anterior surface (approximate to 2x2 cm) were recorded by optical mapping during pacing from the LV epicardium (BCL = 500 milliseconds). Conduction velocities (CVs) at 256 sites were calculated and the ventricular tachycardia/ventricular fibrillation (VT/VF) susceptibility was also assessed by programmed electrical stimulation before and 30 minutes after MI. The amount and distribution of Gj protein connexin-43 was analyzed by immunoblotting and immunohistochemistry.
    ResultsAveraged CV in the ischemic border zone (IBZ) was significantly slower in ACh(-) than in ACh(+) (21 7 vs. 34 +/- 6 cm/s; P &lt; 0.01). Short-coupled extra stimulus further decreased CV of IBZ in ACh(-) (13 +/- 4 cm/s) but did not change that in ACh(+) (34 +/- 5 cm/s), leading to a high incidence of conduction block in IBZ in ACh(-) but not in ACh(+) (83% vs. 0%). VT/VF after MI were induced in ACh(-) but suppressed in ACh(+) (10/12 vs. 3/11; P &lt; 0.01). Connexin-43 in the LV anterior wall was significantly reduced after MI in ACh(-) but not in ACh(+).
    ConclusionACh may suppress VT/VF by preventing loss of Gj and improving CV in IBZ during acute MI.

    DOI: 10.1111/jce.12663

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  • Immunological identification of H<sup>+</sup>-coupled myo-inositol cotransporter in the lateral wall of the cochlear duct.

    Yamaji M, Inoue R, Edamatsu M, Ando M

    Proceedings of Okayama Association for Laboratory Animal Science31   55 - 58   2015年

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  • Real-time phenomenological analysis of dielectric behavior of Euglena cells during their cell-shape changes.

    Hanahara K, Ando M

    Japanese Journal of Protozoology47   4 - 4   2014年

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  • Microtubule dynamics during rapid axopodial contraction in heliozoon Raphidiophrys contractilis revealed by immunoelectron microscopy.

    Inoue R, Ando M

    Japanese Journal of Protozoology47   3 - 3   2014年

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  • Ultrastructural changes during rapid axopodial contraction in heliozoon Raphidiophrys contractilis.

    Inoue R, Edamatsu M, Ando M

    Japanese Journal of Protozoology46   3 - 3   2013年

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  • Physiological significance of intercellular communication mediated by tissue-specific membrane transport systems:insights from heart and inner-ear tissues.

    Ando M

    Proceedings of Okayama Association for Laboratory Animal Science29   55 - 60   2013年

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  • Ultrastructural metamorphosis of the apostome ciliate Vampyrophrya pelagica in the phoront stage during and after infection to pelagic copepods.

    Kanazawa A, Suzaki T, Ando M, Ohtsuka S

    Japanese Journal of Protozoology45   21 - 21   2012年

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  • A prototype of the biomonitoring system for assessing water quality using heliozoon cells.

    Yoshimura C, Ando M, Suzaki T

    Japanese Journal of Protozoology45   33 - 33   2012年

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  • Three-dimensional observation of the vascular networks and functional proteins in the cochlear stria vascularis using a non-corroded casting method combined with an immunohistochemical analysis. 査読

    Edamatsu M, Hishikawa S, Kondo Y, Ando M

    Bioimages20   9 - 15   2012年

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    担当区分:責任著者  

    DOI: 10.11169/bioimages.20.9

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  • Multiple expression of glucose transporters in the lateral wall of the cochlear duct studied by quantitative real-time PCR assay 査読

    Midori Edamatsu, Yasuhiro Kondo, Motonori Ando

    NEUROSCIENCE LETTERS490 ( 1 ) 72 - 77   2011年2月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    We have investigated the gene expression of the facilitated glucose transporter (GLUT), H(+)-coupled myoinositol cotransporter (HMIT), and Na(+) glucose cotransporter (SGLT) in the lateral wall of the cochlear duct by conventional RT-PCR and quantitative real-time PCR. The isoforms GLUT1, -3, -4, -5, -8, -10, -12 and HMIT were detected in both the stria vascularis and the spiral ligament, whereas no SGLT isoforms could be detected in these tissues. Quantitative real-time PCR analysis revealed significant differences in the gene expression of GLUT1, -4, -5, -10, and HMIT isoforms between the stria vascularis and the spiral ligament. This result reflects the tissue-dependent distributions of GLUT isoforms. These findings strongly suggest that a number of GLUT isoforms participate in glucose transport in the stria vascularis and the spiral ligament. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2010.12.029

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  • Steps toward the practical use of a system for water quality monitoring using heliozoon cells

    Yoshimura C, Ando M, Suzaki T

    Japanese Journal of Protozoology44   53 - 54   2011年

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  • Re-elongation of axopodia after induction of rapid axopodial contraction in heliozoon Raphidiophrys contractilis.

    Enomoto T, Suzaki T, Ando M

    Japanese Journal of Protozoology44   58 - 59   2011年

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  • Differential regulation of TNF receptors by vagal nerve stimulation protects heart against acute ischemic injury 査読

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Mikihiko Arikawa, Fumiyasu Yamasaki, Takayuki Sato

    JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY49 ( 2 ) 234 - 244   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD  

    Vagal nerve stimulation (VS) has been reported to improve the survival after both acute and chronic myocardial infarction through the release of neurotransmitter ACh. However, the precise mechanism behind its beneficial effect is still unknown. In this study, we demonstrate the upregulation of tumor necrosis factor-alpha (TNF-alpha) and its cell survival TNF receptor-2 (TNFR2) as the mechanism behind VS induced myocardial protection. We investigated the effects of efferent VS on myocardial ischemic injury with in vivo and in vitro mouse models. In in vivo hearts VS significantly increased the expression of TNF-alpha both at the messenger and protein level after 3-hours of myocardial ischemia. In the in vitro studies ACh treatment before hypoxia, induced a significant upregulation of TNF-alpha compared to the untreated cardiomyocytes. Immunofluorescence analysis confirmed the synthesis of TNF-alpha by cardiomyocytes both in vivo and in vitro. VS also significantly reduced the myocardial infarct size (23.9 +/- 5.7% vs. 56 +/- 1.9%) and activated the cell survival Akt cascade system. Further, ACh upregulated the cell survival TNFR2 expression, while downregulating the cell destructive TNF receptor 1 (TNFR1) expression. These results were confirmed using the TNF receptors deficient mice, where the VS mediated protection was lost both in vivo and in vitro in TNFR2 (TNFR2(-/-)) and TNF receptors double knock out (TNFR1(-/-)2(-/-)) mice. VS and ACh protects the heart against acute ischemia or hypoxic injury by differentially regulating the TNF receptor subtypes. (C) 2010 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.yjmcc.2010.03.007

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  • Engineered Heart Tissue: A Novel Tool to Study the Ischemic Changes of the Heart In Vitro 査読

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Takayuki Sato

    PLOS ONE5 ( 2 ) 1 - 5   2010年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Background: Understanding the basic mechanisms and prevention of any disease pattern lies mainly on development of a successful experimental model. Recently, engineered heart tissue (EHT) has been demonstrated to be a useful tool in experimental transplantation. Here, we demonstrate a novel function for the spontaneously contracting EHT as an experimental model in studying the acute ischemia-induced changes in vitro.
    Methodology/Principal Findings: EHT was constructed by mixing cardiomyocytes isolated from the neonatal rats and cultured in a ring-shaped scaffold for five days. This was followed by mechanical stretching of the EHT for another one week under incubation. Fully developed EHT was subjected to hypoxia with 1% O(2) for 6 hours after treating them with cell protective agents such as cyclosporine A (CsA) and acetylcholine (ACh). During culture, EHT started to show spontaneous contractions that became more synchronous following mechanical stretching. This was confirmed by the increased expression of gap junctional protein connexin 43 and improved action potential recordings using an optical mapping system after mechanical stretching. When subjected to hypoxia, EHT demonstrated conduction defects, dephosphorylation of connexin-43, and down-regulation of cell survival proteins identical to the adult heart. These effects were inhibited by treating the EHT with cell protective agents.
    Conclusions/Significance: Under hypoxic conditions, the EHT responds similarly to the adult myocardium, thus making EHT a promising material for the study of cardiac functions in vitro.

    DOI: 10.1371/journal.pone.0009275

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  • Dielectric behavior of the flagellate Euglena gracilis SM-ZK, a permanent chloroplast-lacking mutant.

    Fukuizumi S, Suzaki T, Ando M

    Japanese Journal of Protozoology43   41 - 42   2010年

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  • Evaluation of an in-vivo bioassay system for microtubule inhibitors with antitumor activity using the heliozoon Actinophrys sol.

    Enomoto T, Ishikawa T, Suzaki T, Ando M

    Japanese Journal of Protozoology43   66 - 67   2010年

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  • Anti-Alzheimer&apos;s Drug, Donepezil, Markedly Improves Long-Term Survival After Chronic Heart Failure in Mice 査読

    Takemi Handa, Rajesh G. Katare, Yoshihiko Kakinuma, Mikihiko Arikawa, Motonori Ando, Shiro Sasaguri, Fumiyasu Yamasaki, Takayuki Sato

    JOURNAL OF CARDIAC FAILURE15 ( 9 ) 805 - 811   2009年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CHURCHILL LIVINGSTONE INC MEDICAL PUBLISHERS  

    Background: We previously reported that chronic vagal nerve Stimulation markedly improved long-term survival after chronic heart failure (CHF) in rats through cardioprotective effects of acetylcholine, independent of the heart rate-slowing mechanism. However, such ail approach is invasive and its safety is unknown in clinical settings. To develop an alternative therapy with a clinically available drug, we examined the chronic effect of oral donepezil, in acetylcholinesterase inhibitor against Alzheimer&apos;s disease, on cardiac remodeling and survival with a murine model of volume-overloaded CHF.
    Methods and Results: Four weeks after surgery of aortocaval shunt, CHF mice were randomized into untreated and donepezil-treated groups. Donepezil was orally given at a dosage of 5 mg.kg(-1).day(-1). After 4 weeks of treatment, we evaluated in situ left ventricular (LV) pressure, ex vivo LV pressure-volume relationships, and LV expression of brain natriuretic peptides (BNP). We also observed Survival for 50 days. When compared with the untreated group, the donepezil-treated group had significantly low LV end-diastolic pressure, high LV contractility, and low LV expression of BNP. Donepezil significantly reduced the heart weight and markedly improved the Survival rate during the 50-day treatment period (54% versus 81%, P &lt; .05).
    Conclusions: Oral donepezil improves Survival of CHF mice through prevention of pumping failure and cardiac remodeling. (J Cardiac Fail 2009;15:805-811)

    DOI: 10.1016/j.cardfail.2009.05.008

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  • Vagal nerve stimulation prevents reperfusion injury through inhibition of opening of mitochondrial permeability transition pore independent of the bradycardiac effect 査読

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Mikihiko Arikawa, Takemi Handa, Fumiyasu Yamasaki, Takayuki Sato

    JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY137 ( 1 ) 223 - 231   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MOSBY-ELSEVIER  

    Background: In spite of recent advances in coronary interventional therapy, reperfusion injury is still considered to be a major problem in patients undergoing surgical procedures, such as bypass grafting. Here we demonstrate a novel therapeutic strategy against ischemia-reperfusion injury: vagally mediated prevention of reperfusion-induced opening of mitochondrial permeability transition pore.
    Methods: We investigated the effects of efferent vagal stimulation on myocardial reperfusion injury with ex vivo and in vitro rat models. In the ex vivo model the hearts were perfused with intact vagal innervation, which allowed us to study the effects of the vagal nerve on the heart without other systemic effects.
    Results: Compared with sham stimulation, vagal stimulation exerted a marked anti-infarct effect irrespective of the heart rate (34% +/- 6% vs 85% +/- 9% at a heart rate of 300 beats/min, 37% +/- 4% vs 43% +/- 5% at a heart rate of 250 beats/min, and 39% +/- 4% vs 88% +/- 7% at a heart rate of 350 beats/min) after a 30-minute period of global ischemia, activated cell-survival Akt cascade, prevented downregulation of the antiapoptotic protein Bcl-2, and suppressed cytochrome-c release and caspase-3 activation. Furthermore, vagal stimulation-treated hearts exhibited a significant improvement in left ventricular developed pressure (78 +/- 5 vs 45 +/- 8 mm Hg) and a significant attenuation in an incremental change in left ventricular end-diastolic pressure during reperfusion. These beneficial effects of vagal stimulation were abolished by a permeability transition pore opener, atractyloside. In the in vitro study with primary-cultured cardiomyocytes, acetylcholine prevented a reoxygenation-induced collapse in mitochondrial transmembrane potential through inhibition of permeability transition pore opening.
    Conclusion: Vagal stimulation would be a potential adjuvant therapy for the rescue of ischemic myocardium from reperfusion injury, and the protective effects are independent of its bradycardiac effects.

    DOI: 10.1016/j.jtcvs.2008.08.020

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  • Continuous dielectric measurement system for monitoring cell-shape dynamics in the flagellate Euglena gracilis.

    Fukuizumi S, Enomoto T, Edamatsu M, Suzaki T, Ando M

    Japanese Journal of Protozoology42   74 - 74   2009年

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  • Improvement of a flow-through type chamber for the aquatic bio-monitoring system using adhesiveness of heliozoon cells to substratum.

    Enomoto T, Fukuizumi S, Edamatsu M, Suzaki T, Ando M

    Japanese Journal of Protozoology42   22 - 23   2009年

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  • Cellular localization of facilitated glucose transporter 1 (GLUT-1) in the cochlear stria vascularis: its possible contribution to the transcellular glucose pathway 査読

    Motonori Ando, Midori Edamatsu, Sho Fukuizumi, Shunji Takeuchi

    CELL AND TISSUE RESEARCH331 ( 3 ) 763 - 769   2008年3月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Immunoreactivity for the facilitated glucose transporter 1 (GLUT-1) has been found in the cochlear stria vascularis, but whether the strial marginal cells are immunopositive for GLUT-1 remains uncertain. To determine the cellular localization of GLUT-1 and to clarify the glucose pathway in the stria vascularis of rats and guinea pigs, immunohistochemistry was performed on sections, dissociated cells, and whole-tissue preparations. Immunoreactivity for GLUT-1 in sections was observed in the basal side of the strial tissue and in capillaries in both rats and guinea pigs. However, the distribution of the positive signals within the guinea pig strial tissue was more diffuse than that in rats. Immunostaining of dissociated guinea pig strial cells revealed GLUT-1 in the basal cells and capillary endothelial cells, but not in the marginal cells. These results indicated that GLUT-1 was not expressed in the marginal cells, and that another isoform of GLUT was probably expressed in these cells. Three-dimensional observation of whole-tissue preparations demonstrated that cytoplasmic prolongations from basal cells extended upward to the apical surface of the stria vascularis from rats and guinea pigs, and that the marginal cells were surrounded by these protrusions. We speculate that these upward extensions of basal cells have been interpreted as basal infoldings of marginal cells in previous reports from other groups. The three-dimensional relationship between marginal cells and basal cells might contribute to the transcellular glucose pathway from perilymph to intrastrial space.

    DOI: 10.1007/s00441-007-0495-2

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  • Dielectric analysis for cell shape changes in the flagellate Euglena gracilis.

    Fukuizumi S, Edamatsu M, Suzaki T, Ando M

    Japanese Journal of Protozoology41   55 - 57   2008年

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  • Progression of ventricular dysfunction after myocardial infarction is prevented by Anti-Alzheimer's disease drug, donepezil 査読

    Arikawa Mikihiko, Katare Rajesh G, Kakinuma Yoshihiko, Handa Takemi, Ando Motonori, Yamasaki Fumiyasu, Sato Takayuki

    CIRCULATION116 ( 16 ) 291   2007年10月

  • Granulocyte colony-stimulating factor activates Wnt signal to sustain gap junction function through recruitment of beta-catenin and cadherin 査読

    Masanorl Kuwabara, Yoshihiko Kakinuma, Rajesh G. Katare, Motonori Ando, Fumiyasu Yamasaki, Yoshinori Doi, Takayuki Sato

    FEBS LETTERS581 ( 25 ) 4821 - 4830   2007年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Our previous study reveals that connexin (Cx) 43 is targeted by ACh to prevent lethal arrhythmia. Granulocyte colony-stimulating factor (G-CSF), used against ischemic heart failure, may be another candidate, however, with unknown mechanisms. Therefore, we investigated the cellular effects of G-CSF. G-CSF activated the Wnt and Jak2 signals in cardiomyocytes, and up-regulated Cx43 protein and phosphorylation levels. In addition, G-CSF enhanced the localization of Cx43, beta-catenin and cadherin on the plasma membrane. G-CSF inhibited the reduction of Cx43 by enhancing Cx43 anchoring and sustained the cell-cell communication during hypoxia. Consequently, GCSF suppressed ventricular arrhythmia induced by myocardial infarction. As a result, G-CSF could be used as a therapeutic tool for arrhythmia. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.febslet.2007.09.007

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  • 圧受容器反射異常の基礎から治療 脊髄刺激による血圧制御 査読

    山崎 文靖, 牛田 亨宏, 横山 武志, 山下 幸一, 安藤 元紀, 佐藤 隆幸

    日本自律神経学会総会プログラム・抄録集59回   43 - 43   2006年11月

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    記述言語:日本語   出版者・発行元:日本自律神経学会  

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  • Electrophysiological characterization of artificially-arranged human myocardial cells 査読

    Arikawa Mikihiko, Katare Rajesh G, Ando Motonori, Kakinuma Yoshihiko, Yamasaki Funnyasu, Davidson Mercy M, Sato Takayuki

    CIRCULATION114 ( 18 ) 291   2006年10月

  • Vagal nerve stimulation attenuates myocardial ischemia - Reperfusion injury by inhibiting the mitochondrial permeability transition pore 査読

    Katare Rajesh G, Ando Motonori, Kakinuma Yoshihiko, Arikawa Mikihiko, Handa Takemi, Yamasaki Fumiyasu, Sasaguri Shiro, Sato Takayuki

    CIRCULATION114 ( 18 ) 1196   2006年10月

  • Engineered heart tissue - A novel tool for studying the acute ischemia induced changes in-vitro 査読

    Katare Rajesh G, Motonori Ando, Kakinuma Yoshihiko, Arikawa Mikihiko, Yamasaki Fumiyasu, Sato Takayuki

    CIRCULATION114 ( 18 ) 12   2006年10月

  • Acetylcholine inhibits the hypoxia-induced reduction of connexin43 protein in rat cardiomyocytes 査読

    Yanan Zhang, Yoshihiko Kakinuma, Motonori Ando, Rajesh G. Katare, Fumiyasu Yamasaki, Tetsuro Sugiura, Takayuki Sato

    JOURNAL OF PHARMACOLOGICAL SCIENCES101 ( 3 ) 214 - 222   2006年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE PHARMACOLOGICAL SOC  

    In a recent study, we demonstrated that vagal stimulation increases the survival of rats with myocardial infarction by inhibiting lethal arrhythmia through regulation of connexin43 (Cx43). However, the precise mechanisms for this effect remain to be elucidated. To investigate these mechanisms and the signal transduction for gap junction regulation, we investigated the effect of acetylcholine (ACh), a parasympathetic nerve system neurotransmitter, on the gap junction component Cx43 using H9c2 cells. When cells were subjected to hypoxia, the total Cx43 protein level was decreased. In contrast, pretreatment with ACh inhibited this effect. To investigate the signal transduction, cells were pretreated with L-NAME, a nitric oxide synthase inhibitor, followed by ACh and hypoxia. L-NAME was found to suppress the ACh effect. However, a NO donor, SNAP, partially inhibited the hypoxia-induced reduction in Cx43. To delineate the mechanisms of the decrease in Cx43 under hypoxia, cells were pretreated with MG132, a proteasome inhibitor. Proteasome inhibition produced a striking recovery of the decrease in the total Cx43 protein level under hypoxia. However, cotreatment with MG132 and ACh did not produce any further increase in the total Cx43 protein level. Functional studies using ACh or okadaic acid, a phosphatase inhibitor, revealed that both reagents inhibited the decrease in the dye transfer induced by hypoxia. These results suggest that ACh is responsible for restoring the decrease in the Cx43 protein level, resulting in functional activation of gap junctions.

    DOI: 10.1254/jphs.FP0051023

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  • Nitric oxide stimulates vascular endothelial growth factor production in cardiomyocytes involved in angiogenesis 査読

    M Kuwabara, Y Kakinuma, M Ando, RG Katare, F Yamasaki, Y Doi, T Sato

    JOURNAL OF PHYSIOLOGICAL SCIENCES56 ( 1 ) 95 - 101   2006年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PHYSIOLOGICAL SOCIETY JAPAN  

    Background: Hypoxia-inducible factor (HIF)-1 alpha regulates the transcription of lines of genes, including vascular endothelial growth factor (VEGF), a major gene responsible for angiogenesis. Several recent studies have demonstrated that a nonhypoxic pathway via nitric oxide (NO) is involved in the activation of HIF-1 alpha. However, there is no direct evidence demonstrating the release of angiogenic factors by cardiomyocytes through the nonhypoxic induction pathway of HIF-1 alpha in the heart. Therefore we assessed the effects of an NO donor, S-Nitroso-N-acetylpenicillamine (SNAP) on the induction of VEGF via HIF-1 alpha under normoxia, using primary cultured rat cardiomyocytes (PRCMs). Methods and Results: PRCMs treated with acetylcholine (ACh) or SNAP exhibited a significant production of NO. SNAP activated the induction of HIF-1 alpha protein expression in PRCMs during normoxia. Phosphatidlylinositol 3-kinase (PI3K)-dependent Akt phosphorylation was induced by SNAP and was completely blocked by wortmannin, a PI3K inhibitor, and N-G-nitro-L-arginine methyl ester (L-NAME), a NO synthase inhibitor. The SNAP treatment also increased VEGF protein expression in PRCMs. Furthermore, conditioned medium derived from SNAP-treated cardiomyocytes phosphorylated the VEGF type-2 receptor (Flk-1) of human umbilical vein endothelial cells (a fourfold increase compared to the control group, p &lt; 0.001, n = 5) and accelerated angiogenesis. Conclusion: Our results suggest that cardiomyocytes produce VEGF through a nonhypoxic HIF-1 alpha induction pathway activated by NO, resulting in angiogenesis.

    DOI: 10.2170/physiolsci.RP002305

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  • Artificial baroreflex - Clinical application of a bionic baroreflex system 査読

    F Yamasaki, T Ushida, T Yokoyama, M Ando, K Yamashita, T Sato

    CIRCULATION113 ( 5 ) 634 - 639   2006年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Background - We proposed a novel therapeutic strategy against central baroreflex failure: implementation of an artificial baroreflex system to automatically regulate sympathetic vasomotor tone, ie, a bionic baroreflex system (BBS), and we tested its efficacy in a model of sudden hypotension during surgery.
    Methods and Results - The BBS consisted of a computer-controlled negative-feedback circuit that sensed arterial pressure (AP) and automatically computed the frequency (STM) of a pulse train required to stimulate sympathetic nerves via an epidural catheter placed at the level of the lower thoracic spinal cord. An operation rule was subsequently designed for the BBS using a feedback correction with proportional and integral gain factors. The transfer function from STM to AP was identified by a white noise system identification method in 12 sevoflurane-anesthetized patients undergoing orthopedic surgery involving the cervical vertebrae, and the feedback correction factors were determined with a numerical simulation to enable the BBS to quickly and stably attenuate an external disturbance on AP. The performance of the designed BBS was then examined in a model of orthostatic hypotension during knee joint surgery (n = 21). Without the implementation of the BBS, a sudden deflation of a thigh tourniquet resulted in a 17 +/- 3 mm Hg decrease in AP within 10 seconds and a 25 +/- 2 mm Hg decrease in AP within 50 seconds. By contrast, during real-time execution of the BBS, the decrease in AP was 9 +/- 2 mm Hg at 10 seconds and 1 +/- 2 mm Hg at 50 seconds after the deflation.
    Conclusions - These results suggest the feasibility of a BBS approach for central baroreflex failure.

    DOI: 10.1161/CIRCULATIONAHA.105.587915

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  • Efferent vagal nerve stimulation protects heart against ischemia-induced arrhythmias by preserving connexin43 protein 査読

    M Ando, RG Katare, Y Kakinuma, DM Zhang, F Yamasaki, K Muramoto, T Sato

    CIRCULATION112 ( 2 ) 164 - 170   2005年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Background - Myocardial ischemia (MI) leads to derangements in cellular electrical stability and the generation of lethal arrhythmias. Vagal nerve stimulation has been postulated to contribute to the antifibrillatory effect. Here, we suggest a novel mechanism for the antiarrhythmogenic properties of vagal stimulation during acute MI.
    Methods and Results - Under anesthesia, Wistar rats underwent 30 minutes of left coronary artery (LCA) ligation with vagal stimulation (MI-VS group, n = 11) and with sham stimulation (MI-SS group, n = 12). Eight of the 12 rats in the MI-SS group had ventricular tachyarrhythmia (VT) during 30-minute LCA ligation; on the other hand, VT occurred in only 1 of the 11 rats in the MI-VS group (67% versus 9%, respectively). Atropine administration abolished the antiarrhythmogenic effect of vagal stimulation. Immunoblotting revealed that the MI-SS group showed a marked reduction in the amount of phosphorylated connexin43 (Cx43), whereas the MI-VS group showed only a slight reduction compared with the sham operation and sham stimulation group (37 +/- 20% versus 79 +/- 18%). Immunohistochemistry confirmed that the MI-induced loss of Cx43 from intercellular junctions was prevented by vagal stimulation. In addition, studies with rat primary-cultured cardiomyocytes demonstrated that acetylcholine effectively prevented the hypoxia-induced loss of phosphorylated Cx43 and ameliorated the loss of cell-to-cell communication as determined by Lucifer Yellow dye transfer assay, which supports the in vivo results.
    Conclusions - Vagal nerve stimulation exerts antiarrhythmogenic effects accompanied by prevention of the loss of phosphorylated Cx43 during acute MI and thus plays a critical role in improving ischemia-induced electrical instability.

    DOI: 10.1161/CIRCULATIONAHA.104.525493

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  • Acetylcholine from vagal stimulation protects cardiomyocytes against ischemia and hypoxia involving additive non-hypoxic induction of HIF-1 alpha 査読

    Y Kakinuma, M Ando, M Kuwabara, RG Katare, K Okudela, M Kobayashi, T Sato

    FEBS LETTERS579 ( 10 ) 2111 - 2118   2005年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Electrical stimulation of the vagal efferent nerve improves the survival of myocardial infarcted rats. However, the mechanism for this beneficial effect is unclear. We investigated the effect of acetylcholine (ACh) on hypoxia-inducible factor (HIF)-1 alpha using rat cardiomyocytes under normoxia and hypoxia. ACh posttranslationally regulated HIF-1 alpha and increased its protein level under normoxia. ACh increased Akt phosphorylation, and wortmannin or atropine blocked this effect. Hypoxia-induced caspase-3 activation and mitochondrial membrane potential collapse were prevented by ACh. Dominant-negative HIF-1 alpha inhibited the cell protective effect of ACh. In acute myocardial ischemia, vagal nerve stimulation increased HIF-1 alpha expression and reduced the infarct size. These results suggest that ACh and vagal stimulation protect cardiomyocytes; through the PI3K/Akt/HIF-1 alpha pathway. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.febslet.2005.02.065

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  • Dielectric behavior of pulmonary edema induced in the rat lung 査読

    T Yamashiro, M Ando, Y Okazaki, S Sasaguri

    RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY145 ( 1 ) 91 - 100   2005年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The dielectric properties (conductivity, kappa and relative permittivity, epsilon) of excised rat lung are modified by lung air and water content. The measurements of these quantities were made over the frequency range of 10 kHz to 100 MHz with an open-ended coaxial probe. The following relationships were analyzed in an oleic acid-induced pulmonary edema model using 18 animals: the spectra Of kappa, epsilon and the loss tangent as a function of lung air and water content. Secondly, an isolated-perfused lung system was produced to induce a gradual increase in lung water. The time course Of kappa, epsilon and the loss tangent for one excised lung was analyzed. The principal findings were: (i) a decrease in kappa and epsilon with increasing air content, (ii) an increase in kappa and epsilon with increasing water content, and (iii) a good correlation between lung water content and maximum loss tangent that was insensitive to changes in air content.
    We conclude that this technique could provide a quantitative assessment of lung water during pulmonary edema formation. (C) 2004 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.resp.2004.08.008

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  • Neural interface therapy for protecting the heart against ischemia-induced arrhythmia and apoptosis.

    Ando M, Katare RG, Kakinuma Y, Sato T

    Proceedings of the Symposium on Biological and Physiological Engineering20   51 - 54   2005年

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  • Effect of electrical modification of cardiornyocytes on transcriptional activity through 5 '-AMP-activated protein kinase 査読

    Y Kakinuma, YN Zhang, M Ando, T Sugiura, T Sato

    JOURNAL OF CARDIOVASCULAR PHARMACOLOGY44   S435 - S438   2004年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Endothelin-1 (ET-1) is known as an aggravating factor of the failing cardiomyocytes and, therefore, a therapeutic method is indispensable to decrease cardiac ET-1 expression. To study the mechanisms of how cardiac ET-1 gene expression can be modified, we investigated the effect of electrical stimulation against cardiomyocytes. Considering the physiology of cardiomyocytes, in vitro cultured cardiomyocytes demonstrate distinctive features from in vivo cardiomyocytes (i.e. the absence of a stretch along with electrical stimulation). In this study, we especially focused on the effect of electrical stimulation. The electrical stimulation reduced the gene expression of ET-1 mRNA in rat primary cultured cardiomyocytes. Furthermore, this effect on the transcriptional modification of ET-1 was also identified in H9c2 cells. Luciferase activity using H9c2 cells was decreased by electrical stimulation in the early phase, suggesting that the attenuation of the ET-1 gene transcription by electrical stimulation should be due to a transcriptional repression. To further investigate a trigger signal involved in the transcriptional repression, phosphorylation of 5'-AMP-activated protein kinase (AMPK) was evaluated. It was revealed that AMPK was phosphorylated in the early phase of electrical stimulation of H9c2 cells as well as in rat primary cultured cardiomyocytes, and that AMPK phosphorylation was followed by ET-1 transcriptional repression, suggesting that electrical stimulation directly regulates AMPK. This study suggests that AMPK activation in cardiomyocytes plays a crucial role in the transcriptional repression of ET-1.

    DOI: 10.1097/01.fjc.0000166318.91623.f9

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  • Carotid-sinus baroreflex modulation of core and skin temperatures in rats: An open-loop approach 査読

    DM Zhang, M Ando, F Yamasaki, T Sato

    JAPANESE JOURNAL OF PHYSIOLOGY53 ( 6 ) 461 - 466   2003年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    The neural mechanisms of the thermoregulatory control of core and skin temperatures in response to heat and cold stresses have been well clarified. However, it has been unclear whether baroreceptor reflexes are involved in the control of core and skin temperatures. To investigate how the arterial baroreceptor reflex modulates the body temperatures, we examined the effect of pressure changes of carotid sinus baroreceptors on core and skin temperatures in halothane-anesthetized rats. To open the barore-flex loop and control arterial baroreceptor pressure (BRP), we cut vagal and aortic depressor nerves and isolated carotid sinuses. We sequentially altered BRP in 20-mmHg increments from 60 to 180 mmHg and then in 20-mmHg decrements from 180 to 60 mmHg while measuring systemic arterial pressure (SAP), heart rate (HR), and core blood temperature (T-core) at the aortic arch and skin temperature (T-skin) at the tail. In response to the incremental change in BRP by 120 mmHg, SAP, HR, and T-core fell by 90.3+/-5.1 mmHg, 60.3+/-10.5 beats min(-1), and 0.18+/-0.01degreesC, respectively. Tskin rose by 0.84+/-0.10degreesC. The maximum rate of change per unit BRP change was -2.1+/-0.2 for SAP, -1.5+/-0.4 beats min(-1) mmHg(-1) for HR, -0.003+/-0.001degreesC mmHg(-1) for Tcore, and 0.011+/-0.002degreesC mmHg(-1) for T-skin. After the administration of hexamethonium or bretylium, these baroreflexogenic responses were completely abolished. We concluded that Tcore and Tskin are modulated by the arterial baroreceptor reflex.

    DOI: 10.2170/jjphysiol.53.461

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  • Aquaporin-1 (AQP1) is expressed in the stria vascularis of rat cochlea 査読

    S Sawada, T Takeda, H Kitano, S Takeuchi, T Okada, M Ando, M Suzuki, A Kakigi

    HEARING RESEARCH181 ( 1-2 ) 15 - 19   2003年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Cochlea endolymph, produced by the stria vascularis, is essential for normal inner ear function. Abnormal endolymphatic volumes correlate closely with pathological conditions such as Meniere's disease. The critical roles played by aquaporins, which facilitate osmotic movement of water molecules, are known in a variety of tissues. We investigated the expression of aquaporin-1 (AQP1) in the rat inner ear using reverse transcription polymerase chain reaction and immunohistochemical methods. We obtained novel data showing that not just AQP1 mRNA but also AQP1 protein is expressed in the stria vascularis, in addition to other data confirming previous reports. AQP1 immuno reactivity localized to the intermediate cells in the stria vascularis. The above finding suggests that AQP1 may play a role in the water distribution associated with vigorous ion transport in the stria vascularis since the intermediate part of the stria vascularis contains both intermediate cells and the basolateral parts of marginal cells, both of which express ion transporters abundantly. (C) 2003 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0378-5955(03)00131-X

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  • Acute ischemia causes ‘dark cell’ change of strial marginal cell in gerbil cochlea. 査読

    Ando M, Takeuchi S, Kakigi A, Raicu V, Yagyu K, Sato T

    Cell and Tissue Research309 ( 2 ) 229 - 235   2002年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s00441-002-0597-9

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  • Reduction in the endocochlear potential caused by Cs+ in the perilymph can be explained by the five-compartment model of the stria vascularis 査読

    A Kakigi, S Takeuchi, M Ando, K Higashiyama, H Azuma, T Sato, T Takeda

    HEARING RESEARCH166 ( 1-2 ) 54 - 61   2002年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    In an earlier publication (Takeuchi et al., Biophys. J. 79 (2000) 2572 2582), we proposed that K channels in intermediate cells within the stria vascularis may play an essential role in the generation or the endocochlear potential (EP). and we presented an extended version of the five-compartment model of the stria vascularis. In search of further evidence supporting the five-compartment model, we studied the effects of Cs+ added to the perilymph on guinea pig EP, Cs+ is known as a competitive K+ channel blocker. Both the scala tympani and the scala vestibuli of four cochlear turns were perfused at a flow rate of 10 mul/min, and the EP was recorded from the second cochlear turn. Cs- at 30 mM caused a biphasic change in the EP the EP increased transiently from a control level of 89.6 mV to 94.8 mV within 10 min, and then decreased to a. steady level of 24.5 mV within the next 40 min. We propose that the initial transient increase in the EP results from Cs+-mediated blockade of K+ conductance in the basolateral membrane of hair cells. and that the subsequent EP decrease is due to effects of Cs+ on the stria vascularis. We believe that Cs- in the perilymph is able to access the stria vascularis by being taken up by fibrocytes in the spiral ligament and then being transported to intermediate cells because it is known that Cs+ is taken up via Na+, K+-ATPase and that gap junctions connect fibrocytes in the spiral ligament to basal cells and basal cells to intermediate cells. To clarify the effect of intracellular Cs+ on the electrophysiological properties of intermediate cells. these cells were dissociated from guinea pigs and Studied by the whole-cell patch-clamp method. intracellular Cs+ depolarized intermediate cells in a dose-dependent manner. In addition, efflux of Cs+ from the intermediate cell was much less than the efflux of K+. Thus, Cs+ may accumulate in the intermediate cell, which depolarizes the cell, which in turn decreases the EP. We conclude that the five-compartment model of the stria vascularis can explain the EP decrease caused by Cs+ in the perilymph. (C) 2002 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0378-5955(01)00412-9

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  • Reduction in the endocochlear potential caused by Cs+ in the perilymph can be explained by the five-compartment model of the stria vascularis.

    Ando M, Takeuchi S, Kakigi A, Raicu V, Sato T

    Cell Tissue Res309   229 - 235   2002年

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  • Bumetanide-induced enlargement of the intercellular space in the stria vascularis requires an active Na+-K+-ATPase 査読

    H Azuma, S Takeuchi, K Higashiyama, M Ando, A Kakigi, M Nakahira, K Yamakawa, T Takeda

    ACTA OTO-LARYNGOLOGICA122 ( 8 ) 816 - 821   2002年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS AS  

    Objective-Loop diuretics such as bumetanide and furosemide cause an acute enlargement of the intrastrial space of the stria vascularis, with an associated decline in the endocochlear DC potential (EP). The aim of this study was to determine the role played by the Na+-K+-ATPase in the bumetanide-induced enlargement of the intrastrial space, and to examine the importance of the balance between the activities of the Na+-K+-2Cl(-) cotransporter and the Na+-K+-ATPase to the physiological function of the stria vascularis.
    Material and methods-Albino guinea pigs were used in experiments involving perilymphatic perfusion, EP measurement and electron microscopy. The effects of bumetanide on the stria vascularis were examined following inhibition of the Na+-K+-ATPase by ouabain. Ouabain was administered to the perfusate and, when the EP reached 0 mV, both ouabain and bumetanide were administered.
    Results-Although there was no enlargement of the intrastrial space, vacuoles were apparent in marginal cells. The vacuolar change in marginal cells was similar to that caused by ouabain alone.
    Conclusion-This study indicates that the enlargement of the intrastrial space requires not only the blockade of the Na+-K+-2Cl(-) cotransporter but also normal activity of the Na+-K+-ATPase, and suggests that the bumetanide-induced enlargement of the intrastrial space resulted from the imbalance between the activities of the Na+-K+-2Cl(-) cotransporter and the Na+-K+-ATPase.

    DOI: 10.1080/003655402/000028051

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  • Three-dimensional and ultrastructural relationships between intermediate cells and capillaries in the gerbil stria vascularis 査読

    S Takeuchi, M Ando, T Sato, A Kakigi

    HEARING RESEARCH155 ( 1-2 ) 103 - 112   2001年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Structural relationships between intermediate cells and capillaries in the stria vascularis of gerbils were examined by confocal laser microscopy and electron microscopy. Immunustaining for an inward rectifier K+ channel (Kir4.1), which was localized to intermediate cells, was used to determine the three-dimensional distribution of intermediate cells. These cells constituted a honeycomb-like network, and their dendritic processes surrounded not only capillaries but also the basolateral surface of epithelial marginal cells. On the basis of the above finding and the large K+ conductance in intermediate cells, M e propose that the network composed of intermediate cells has a spatial K+ buffering function. Transmission electron microscopy revealed the absence of the basal lamina in some regions and the presence of a gap junction-like membrane association between intermediate cells and pericytes: and/or endothelial cells. This: result supported our previous finding that intermediate cells were dye-coupled with pericytes and endothelial cells. The presence of gap junctions between intermediate cells and pericytes and/or endothelial cells suggests that endothelial cells and pericytes may play roles other than forming a structural route for blood circulation. (C) 2001 Elsevier Science B.V. All rights reserved.

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  • Mechanism generating endocochlear potential: Role played by intermediate cells in stria vascularis 査読

    S Takeuchi, M Ando, A Kakigi

    BIOPHYSICAL JOURNAL79 ( 5 ) 2572 - 2582   2000年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOPHYSICAL SOCIETY  

    The endocochlear DC potential (EP) is generated by the stria vascularis, and essential for the normal function of hair cells. Intermediate cells are melanocytes in the stria vascularis. To examine the contribution of the membrane potential of intermediate cells (E-m) to the EP, a comparison was made between the effects of K+ channel blockers on the E-m and those on the EP. The E-m of dissociated guinea pig intermediate cells was measured in the zero-current clamp mode of the whole-cell patch clamp configuration. The E-m changed by 55.1 mV per 10-fold changes in extracellular K+ concentration. Ba2+, Cs+, and quinine depressed the E-m in a dose-dependent manner, whereas tetraethylammonium at 30 mM and 4-aminopyridine at 10 mM had no effect. The reduction of the E-m by Ba2+ and Cs+ was enhanced by lowering the extracellular K+ concentration from 3.6 mM to 1.2 mM. To examine the effect of the K+ channel blockers on the EP, the EP of guinea pigs was maintained by vascular perfusion, and K+ channel blockers were administered to the artificial blood. Ba2+, Cs+ and quinine depressed the EP in a dose-dependent manner, whereas tetraethylammonium at 30 mM and 4-aminopyridine at 10 mM did not change the EP. A 10-fold increase in the K+ concentration in the artificial blood caused a minor decrease in the EP of only 10.6 mV. The changes in the EP were similar to those seen in the E-m obtained at the lower extracellular K+ concentration of 1.2 mM. On the basis of these results, we propose that the EP is critically dependent on the voltage jump across the plasma membrane of intermediate cells, and that K+ concentration in the intercellular space in the stria vascularis may be actively controlled at a concentration lower than the plasma level.

    DOI: 10.1016/S0006-3495(00)76497-6

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  • mRNA encoding 'CIC-K1, a kidney Cl--channel' is expressed in marginal cells of the stria vascularis of rat cochlea: its possible contribution to Cl- currents 査読

    M Ando, S Takeuchi

    NEUROSCIENCE LETTERS284 ( 3 ) 171 - 174   2000年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    The cochlear stria vascularis is essential for the normal function of hair cells, mRNA encoding the CIC-K1 Cl- channel, previously thought to be found only in the kidney, was detected in epithelial marginal cells of the rat stria vascularis by single cell RT-PCR. When Cl- currents were recorded from rat marginal cells by the whole-cell patch clamp method, the steady-state currents showed weak outward rectification and an ion selectivity sequence of SCN- &gt; Br- = Cl- &gt; F- &gt; NO3- &gt; I- &gt; gluconate(-). The Cl- currents were regulated by extracellular Ca2+ and pH. These characteristics resemble those reported for the currents recorded from Xenopus oocytes expressing CIC-K1 Cl- channels. These data together suggest that CIC-K1 Cl- channels may contribute to the whole-cell currents of marginal cells. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

    DOI: 10.1016/S0304-3940(00)01021-1

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  • Immunological identification of an inward rectifier K+ channel (Kir4.1) in the intermediate cell (melanocyte) of the cochlear stria vascularis of gerbils and rats 査読

    M Ando, S Takeuchi

    CELL AND TISSUE RESEARCH298 ( 1 ) 179 - 183   1999年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER VERLAG  

    The cochlear stria vascularis produces the positive endocochlear potential (EP) and the endolymph. Both the EP and the endolymph are essential for the physiological function of hair cells. The intermediate cell is one of several cell types constituting the stria vascularis. It is known that inward rectifier K+ channels can play a constitutive role in the determination of the resting membrane potential. Localization of a member of the inward rectifier K+ channel family, Kir4.1, in the stria vascularis of gerbils and rats was investigated by immunological methods. A polyclonal antibody specific to the C-terminus of the rat Kir4.1 channel was raised in rabbits. Immunostaining of dissociated cells revealed that the Kir4.1 channel was localized to the intermediate cell, but not to the epithelial marginal cell. Subcellular localization of the Kir4.1 channel to the plasma membrane of the intermediate cell was confirmed by immunoelectron microscopy. Immunostaining of whole-tissue preparations revealed a network-like structure composed of intermediate cells. It seems likely that the Kir4.1 channel mediates the inwardly rectifying K+ current in the intermediate cell as shown previously by electrophysiological methods, and that this channel plays key roles in the production of the EP and K+ transport in the stria vascularis.

    DOI: 10.1007/s004419900066

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  • Voltage-dependent outward K+ current in intermediate cell of stria vascularis of gerbil cochlea 査読

    S Takeuchi, M Ando

    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY277 ( 1 ) C91 - C99   1999年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    A voltage-dependent outward KC (Kv) current in the intermediate cell (melanocyte) of the cochlear stria vascularis was studied using the whole cell patch-clamp technique. The Ky current had an activation threshold voltage of approximately -80 mV, and 50% activation was observed at -42.6 mV. The time courses of activation and inactivation were well fitted by two exponential functions: the time constants at 0 mV were 7.9 and 58.8 ms for activation and 0.6 and 4.3 s for inactivation. The half-maximal activation time was 13.8 ms at 0 mV. Inactivation of the current was incomplete even after a prolonged depolarization of 10 s. This current was independent of intracellular Ca2+. Quinine, verapamil, Ba2+, and tetraethylammonium inhibited the current in a dose-dependent manner, but 4-aminopyridine was ineffective at 50 mM. We conclude that the Ky conductance in the intermediate cell may stabilize the membrane potential, which is thought to be closely related to the endocochlear potential, and may provide an additional route for K+ secretion into the intercellular space.

    DOI: 10.1152/ajpcell.1999.277.1.C91

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  • Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats 査読

    M Ando, A Kakigi, S Takeuchi

    CELL AND TISSUE RESEARCH296 ( 3 ) 673 - 676   1999年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER VERLAG  

    Bridging structures between discrete capillaries in the stria vascularis of the cochlea were studied morphologically in gerbils and rats. Serial thin sections for transmission electron microscopy revealed (1) that elongated cells surrounded by the basal lamina provided the structural basis for the bridging structure, (2) that the basal lamina surrounding the elongated cell extended to the basal lamina around the capillary endothelial cell, (3) that the electron density of the cytoplasm was similar to that of the pericytes around the capillaries, and (4) that the cell was attached to the capillaries at both ends only. Visualization of the basal lamina by immunofluorescent methods revealed (1) that capillaries were often bent at the site of attachment of the bridging cell, (2) that the bridging cell bifurcated occasionally, and (3) that the density of the bridging cell was much higher in the stria vascularis than in the underlying spiral ligament. Filamentous actin visualized by fluorescent phalloidin was not apparent in the bridging cell. We propose that the bridging cell provides mechanical strength to the tortuous capillary network in the stria vascularis and participates in the specific function of the stria vascularis in cooperation with other types of cells.

    DOI: 10.1007/s004410051327

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  • Postnatal vascular development in the lateral wall of the cochlear duct of gerbils: quantitative analysis by electron microscopy and confocal laser microscopy 査読

    M Ando, S Takeuchi

    HEARING RESEARCH123 ( 1-2 ) 148 - 156   1998年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The development of the capillary network in the stria vascularis and in the underlying spiral ligament of gerbils was systematically and quantitatively investigated by conventional electron microscopy and confocal laser microscopy in association with vascular labeling with fluorescent gelatin. The developmental changes of capillaries in the lateral wall were observed as the following series of events. (i) At 0 days after birth (DAB) capillaries already existed in the spiral ligament as a network. (ii) At 3-9 DAB the capillary network developed into two layers starting from the scala vestibuli side to the scala tympani side; one layer was located in the stria and the other in the spiral ligament. (iii) At 9 DAB capillaries in the stria became separated from the spiral ligament, and the capillary network consisting of a two-layered structure was complete. (iv) Total capillary length and capillary density in the lateral wall increased until 9 DAB and leveled off thereafter, but changes in the relative position of capillaries in the stria toward the luminal surface of marginal cells continued until 31 DAB. On the basis of the above observations, we propose two possible mechanisms underlying the vascular development in the lateral wall: (i) the formation of new vasculature (angiogenesis), and (ii) changes in the position of cellular components relative to capillaries in association with the differentiation and maturation of marginal cells and intermediate cells. (C) 1998 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0378-5955(98)00109-9

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  • Dye-coupling of melanocytes with endothelial cells and pericytes in the cochlea of gerbils 査読

    S Takeuchi, M Ando

    CELL AND TISSUE RESEARCH293 ( 2 ) 271 - 275   1998年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER VERLAG  

    Intercellular connections via gap junctions in the stria vascularis, which constitutes the lateral wall of the cochlear duct, were investigated by the Lucifer yellow microinjection method with the aid of a confocal laser microscope. The dye injected into an intermediate cell (melanocyte) diffused into capillary endothelial cells and pericytes as well as other intermediate cells, basal cells, and fibrocytes in the spiral ligament; whereas the dye injected into a marginal cell (epithelial cell) was confined to the injected cell. The observation of dye-coupling between intermediate cells and endothelial cells and pericytes makes likely the possibility that these cells work together to play a role in the specific function of the stria vascularis (i.e., production of the positive endocochlear potential and the endolymph) and adds endothelial cells and pericytes to the current "two-cell model" of the stria vascularis.

    DOI: 10.1007/s004410051118

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  • Inwardly rectifying K+ currents in intermediate cells in the cochlea of gerbils: a possible contribution to the endocochlear potential 査読

    S Takeuchi, M Ando

    NEUROSCIENCE LETTERS247 ( 2-3 ) 175 - 178   1998年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    The stria vascularis in the cochlea generates the endocochlear potential (EP) and secretes K+-rich endolymph; both are indispensable for normal sound transduction by hair cells. K+ conductance in the intermediate cell, one of the several types of cells constituting the stria vascularis, was investigated by the whole-cell patch-clamp technique. Inwardly-rectifying K+ (K-ir) currents were the major currents observed. The currents were inhibited dose-dependently by Ba2+, quinine, verapamil and Cs+, but not by tetraethylammonium (20 mM), 4-aminopyridine (5 mM) or Cd2+ (1 mM). The similarity between the effect of inhibitors on K-ir currents and on the EP (Takeuchi et al., Hearing Res., 101 (1996) 181-185) suggests a direct contribution of the K-ir conductance to the generation of the EP. (C) 1998 Elsevier Science Ireland Ltd.

    DOI: 10.1016/S0304-3940(98)00318-8

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  • Marginal cells of the stria vascularis of gerbils take up glucose via the facilitated transporter GLUT: application of autofluorescence 査読

    S Takeuchi, M Ando

    HEARING RESEARCH114 ( 1-2 ) 69 - 74   1997年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Strial marginal cells are known to take up and metabolize glucose as their main source of metabolic energy. The membrane transport mechanisms for glucose uptake into strial marginal cells, however, are largely unknown. Two types of glucose transporters in mammalian cells have been described, the facilitated glucose transporter GLUT and the sodium/glucose cotransporter SGLT. The goal of the present study was to determine which of these represent the main glucose uptake mechanism in strial marginal cells. Glucose uptake into strial marginal cells was assessed by monitoring the cellular concentration of the reduced form of nicotinamide adenine dinucleotide (NADH) fluorometrically. The relation between the autofluorescence from marginal cells and cellular metabolism was verified as follows. The autofluorescence (excitation: 340 nm, emission: 450-490 nm) decreased when oxidative phosphorylation in the mitochondria was uncoupled with carbonyl cyanide 3-chlorophenylhydrazone (CCCP) and increased when cytochrome oxidase was inhibited with cyanide. These effects indicate that the autofluorescence is dependent on the mitochondrial metabolic state, and more specifically on the level of NADH in mitochondria. Glucose removal from the bath solution elicited a 39% decrease in the autofluorescence intensity within 5 min. Similarly, cytochalasin B (10 mu M) reduced the fluorescence intensity by 34% in 5 min. In contrast, neither phlorizin (0.1 mM) nor Na+ removal from the bath solution caused any appreciable change in the autofluorescence intensity. These results indicate that glucose depletion affects the metabolic state of the marginal cell within a few minutes, and that marginal cells take up glucose via GLUT, but not via SGLT. Since the excitation and emission wavelengths of several fluorescent dyes used in physiological studies (e.g., Fura-2 and SBFI) are similar to those of NADH, possible effects of autofluorescence on recording signals should always be taken into account when these dyes are utilized. (C) 1997 Elsevier Science B.V.

    DOI: 10.1016/S0378-5955(97)00157-3

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  • Changes in the volume of marginal cells induced by isotonic 'Cl- depletion/restoration': involvement of the Cl- channel and Na+-K+-Cl- cotransporter 査読

    S Takeuchi, M Ando, A Irimajiri

    HEARING RESEARCH113 ( 1-2 ) 99 - 109   1997年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Marginal cells constitute the endolymph-facing epithelium responsible for the secretion of endolymph by the stria vascularis in the inner ear. We have studied the possible involvement of Cl- conductance and Na+-K+-Cl- cotransport in the mechanism of changes in cell volume upon isotonic Cl- depletion/restoration. Changes in cell volume were estimated from video-microscopic images with the aid of an image processor. Marginal cells shrank to similar to 80% of their original volume in 30 s and to 65-70% in 90 s upon total replacement of [Cl](0) (similar to 150 mM) by gluconate(-), and the original volume of the shrunken cells was restored within 2 min after restoration of Cl-. The order of potency of anions to induce isotonic shrinkage was gluconate(-) &gt; I- &gt; F- &gt; Br-. The cell shrinkage caused by Cl- depletion was partially inhibited by 5-Nitro-2-(3-phenyl-propylamino)-benzoic acid (NPPB, 0.2 mM), but not by either 4-acetamido-4'-isothiocyanato-stilbene-2,2'disulfonic acid (SITS, 0.5 mM), bumetanide (10 mu M) or ouabain (1 mM). The cell shrinkage caused by a reduction of [Cl](0) from similar to 150 mM to 7.5 mM was not affected by [K](0) in the range of 3.6 mM to 72 mM. These results suggest that the main efflux pathway(s) responsible for the 'Cl removal'-induced shrinkage depends on volume-correlated Cl- conductance (Takeuchi and Irimajiri, J. Membrane Biol. 150, 47-62, 1996) and that this pathway(s) is essentially independent of the Na+-K+-Cl- cotransporter, the Na+,K+-ATPase, and the K+-Cl- cotransporter. With regard to volume recovery after isotonic shrinkage, its critical dependence on the simultaneous presence of Na+, K+ and Cl- in the bath and its substantial inhibition by bumetanide (10 mu M) both indicate a major role for Na+-K+-Cl- cotransport. The strong influence on cell volume of solute fluxes working through the Cl- channel and the Na+-K+-Cl- cotransporter implies an essential role for these pathways in the ion transport mechanism(s) of the marginal cell.

    DOI: 10.1016/S0378-5955(97)00134-2

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  • Dielectric monitoring of rouleaux formation in human whole blood: A feasibility study 査読

    A Irimajiri, M Ando, R Matsuoka, T Ichinowatari, S Takeuchi

    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS1290 ( 3 ) 207 - 209   1996年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    In search of a method for detecting rouleaux formation in vitro, we studied the dielectric behavior of human blood under both agitated and stationary conditions. Among the parameters examined, relative permittivity ('dielectric constant') at 50-100 kHz was found to be a suitable measure of rouleaux growth, which has been difficult to quantify through conventional optical approaches. The electrical method presented here appears applicable to the kinetic analysis of rouleaux formation in undiluted whole blood.

    DOI: 10.1016/0304-4165(96)00048-7

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  • ION CHANNELS IN BASOLATERAL MEMBRANE OF MARGINAL CELLS DISSOCIATED FROM GERBIL STRIA VASCULARIS 査読

    S TAKEUCHI, M ANDO, K KOZAKURA, H SAITO, A IRIMAJIRI

    HEARING RESEARCH83 ( 1-2 ) 89 - 100   1995年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The basolateral membrane of isolated strial marginal cells has been probed for conductive pathways by the patch-damp technique. Two types of voltage-insensitive channels were identified in both cell-attached and excised patches. Of these, frequently (69% of excised patches) observed was a Ca2+-activated nonselective cation channel having a unit conductance of 24.9 +/- 0.5 pS (N = 16). Other characteristics of this type in excised patches include: 1) linear I-V relations with 150 mM K+ (pipette)/150 mM Na+ (bath), 2) a permeability sequence of NH4+ &gt; Na+ = K+ = Rb+ &gt; Li+, 3) a flickering block by quinine or quinidine (both 1 mM), and 3) a dose dependent block of its activity by ADP or ATP (IC50,ATP/IC50,ADP = 20-35), both from the cytosolic side. Channels with similar characteristics were found in the apical membrane of the same cell; however, the basolateral channels were 2-4 times more densely distributed than the apical counterparts. Also frequently (57%) detected was a Cl- channel of 80.0 +/- 0.5 pS (N = 6), whose activity was Ca2+ independent. Additionally, this Cl- channel had: 1) linear I-V relations with symmetric Cl-, 2) a permeability sequence of Cl- &gt; Br- &gt; I- greater than or equal to NO3- greater than or equal to gluconate(-), and 3) a complete and reversible block by 1 mM diphenylamine-2-carboxylate. In contrast to the apical Cl- channels, the basolateral ones had a much higher density (57% vs. &lt; 1%) as well as a higher unit conductance (80 pS vs. 50 pS) than the apical counterpart. The relative abundance of these two types as the major conductive pathways for Na+, K+, and Cl- in the basolateral region must be taken into account when addressing the role of strial marginal cells in generating the positive endocochlear potential. The Cl- channel may facilitate Cl- distribution across the basolateral membrane.

    DOI: 10.1016/0378-5955(94)00191-R

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  • STRUCTURE AND FUNCTION OF THE CYTOSKELETON IN HELIOZOA .3. RAPID MICROTUBULE DISORGANIZATION DURING AXOPODIAL CONTRACTION IN ECHINOSPHAERIUM 査読

    T SUZAKI, M ANDO, Y INAI, Y SHIGENAKA

    EUROPEAN JOURNAL OF PROTISTOLOGY30 ( 4 ) 404 - 413   1994年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    The heliozoan Echinosphaerium feeds on various kinds of small protozoans by slow retraction or rapid contraction of microtubule-containing axopodia. The axonemal microtubules showed an intense positive birefringence that was clearly visualized when the organism was observed under a highly sensitive polarization microscope. In order to know if the axopodial contraction involves rapid disassembly of the cytoskeletal microtubules, dynamic changes of the axonemal birefringence were analyzed here during the process of rapid axopodial contraction by video microscopy with an image digitizing system. The number of microtubules in an axopodium was estimated from its birefringence by applying dielectric theories for shell-covered ellipsoids in concentrated suspensions. Quantitative analysis showed that the fractional volume occupied by the microtubules increased at the proximal region of the axopodium concomitantly with the axopodial contraction, and it was maintained for at least 10 s after the contraction. Electron microscopic observations showed that two bundles of axonemal microtubules crossed each other in the proximal part of a contracted axopodium. These results indicate that, during rapid axopodial contraction, the distal portion of the axonemal microtubules moves as a bundle into the axopodial base without a distinct disassembly of the microtubules themselves.

    DOI: 10.1016/S0932-4739(11)80215-4

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  • Dielectric behavior of the rabbit cornea as a measure of the healing process in injured epithelium 査読

    Y. Mokudai, M. Watanabe, M. Ando, H. Ueno

    Journal of Japanese Ophthalmological Society98 ( 3 ) 215 - 223   1994年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    To correlate changes in the passive electrical properties of the rabbit cornea with quantitative grading of corneal injuries induced by topical application of 70% ethanol, we measured ocular tissue impedances using a surface electrode over the range of 104 ~ 108 Hz and followed their temporal changes for up to 17 days. Dielectric measurements on control eyes yielded a broad dispersion curve, which, in loss tangent terms, could be decomposed into two components: dispersion 1 on the low-frequency side and dispersion 2 on the high-frequency side. By defining the peak value of the total dispersion as P(t) and those of subdispersions 1 and 2 P1 and P2, the ratios, P1/P(t) and P2/P(t), were found to serve as useful indices. Upon appearance of corneal erosion due to ethanol, P1/P(t) markedly increased and returned to the control level with re-epithelialization of the cornea, and the time course of P2/P(t) showed a mirror image to that of P1/P(t). Both ratios correlated well with the erosion area determined photographically. These results indicate that dielectric spectroscopy is applicable to the assessment of the extent and severity of corneal injury.

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  • Dielectric measurements on the rabbit cornea using a surface electrode 査読

    M. Watanabe, Y. Mokudai, H. Ueno, M. Ando, A. Irimajiri

    Journal of Japanese Ophthalmological Society97 ( 5 ) 569 - 574   1993年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • STRUCTURE AND FUNCTION OF THE CYTOSKELETON IN HELIOZOA .2. MEASUREMENT OF THE FORCE OF RAPID AXOPODIAL CONTRACTION IN ECHINOSPHAERIUM 査読

    T SUZAKI, M ANDO, K ISHIGAME, Y SHIGENAKA, M SUGIYAMA

    EUROPEAN JOURNAL OF PROTISTOLOGY28 ( 4 ) 430 - 433   1992年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:VCH PUBLISHERS INC  

    The large heliozoan Echinosphaerium extends a number of needle-like axopodia by which it captures food organisms. Every axopodium contains a bundle of several hundreds of axonemal microtubules as a cytoskeletal element. When the tip of a poly-L-lysine-coated glass micro-needle came into contact with the distal part of an axopodium, a rapid axopodial contraction (2.6 mm/s) occurred with a concomitant bending of the needle toward the cell body. In this report, we measured the force of the axopodial contraction by utilizing the relation between force and bending displacement of the micro-needle, and examined a possibility that the axopodial contraction is ascribed to the axopodial tension (surface tension and/or cytoplasmic elasticity) that is developed as a result of microtubule degradation. The force of the axopodial contraction was estimated in the order of 10(-9) N. Treatment with 10 mM colchicine induced disassembly of the axopodial microtubules and a resulting slow retraction of the axopodia (0.1 mum/s) occurred. The force of the slow retraction was also measured by the same procedure to estimate the axopodial tension, and was in the order of 10(-11) N. It was thus demonstrated that the motive force for axopodial contraction cannot be explained as an axopodial tension generated as a result of disassembly of the microtubules.

    DOI: 10.1016/S0932-4739(11)80007-6

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  • A computer program for estimating size distribution of spherical organelles from electron micrographs.

    Suzaki T, Ando M

    Memoirs of the Faculty of Integrated Arts and Sciences, Hiroshima University, Ser.IV18   35 - 42   1992年

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  • STRUCTURE AND FUNCTION OF THE CYTOSKELETON IN HELIOZOA .1. MECHANISM OF RAPID AXOPODIAL CONTRACTION IN ECHINOSPHAERIUM 査読

    M ANDO, Y SHIGENAKA

    CELL MOTILITY AND THE CYTOSKELETON14 ( 2 ) 288 - 301   1989年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    DOI: 10.1002/cm.970140214

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  • ULTRASTRUCTURE OF THE ISOLATED MICROTUBULES AND INTERMICROTUBULAR BRIDGES IN A HETEROTRICHOUS CILIATE, SPIROSTOMUM-AMBIGUUM 査読

    Y SHIGENAKA, J HOSOI, M ANDO, M ISHIDA, ISHII, I

    JOURNAL OF ELECTRON MICROSCOPY38 ( 5 ) 363 - 370   1989年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC ELECTRON MICRO BUS CENTER ACAD SOC JAPAN  

    DOI: 10.1093/oxfordjournals.jmicro.a050750

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  • 耳の中のアンプ,血管条辺縁細胞の機能を探る.

    山地真裕美, 安藤元紀

    臨床検査57   828   2013年

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  • 聴覚機能におけるエネルギー供給システムの解明.

    安藤元紀

    生物学に関する試験研究論叢25   74 - 78   2010年

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  • Donepezilを使用した容量負荷心不全マウスにおける心機能改善効果, 生存率改善効果の検討

    半田 武巳, カタレ ラジェシュG, 柿沼 由彦, 有川 幹彦, 安藤 元紀, 山崎 文靖, 佐藤 隆幸, 笹栗 志朗

    心臓41   115 - 123   2009年

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  • 不規則チルト負荷試験による圧受容器反射の動特性の推定~マウスからヒトまで.

    安藤元紀, 山崎文靖, 佐藤隆幸

    心臓40   34 - 36   2008年

  • 脊髄刺激による血圧制御.

    山崎文靖, 牛田享宏, 横山武志, 山下幸一, 安藤元紀, 佐藤隆幸

    自律神経44   236 - 242   2007年

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  • 不規則チルト法と平衡線図解析法を用いた圧受容器反射機能の評価.

    安藤元紀, 山崎文靖, 佐藤隆幸

    自律神経44   229 - 235   2007年

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  • Intermittent stretch-induced formation of gap junction between fibrocytes and cardiomyocytes improves dyssynchronous contraction circular engineered heart tissue

    Motonori Ando, Rajesh G. Katare, Yoshihiko Kakinuma, Fumiyasu Yamasaki, Takayuki Sato

    CIRCULATION114 ( 18 ) 80 - 80   2006年10月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

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  • Donepezil, acetylcholinesterase inhibitor, promotes angiogenesis over infarct area in rats after chronic coronary occlusion

    M Ando, RG Katare, Y Kakinuma, MH Li, C Zheng, H Yamasaki, T Satoh

    CIRCULATION112 ( 17 ) U342 - U342   2005年10月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

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  • Vagal nerve stimulation differentially regulates TNF receptors and protect the heart against acute ischemic injury

    RG Katare, M Ando, Y Kakinuma, H Yamasaki, T Satoh

    CIRCULATION112 ( 17 ) U251 - U252   2005年10月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

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  • Vagal stimulation suppresses ischemia-induced arrhythmias by preserving connexin43 protein from dephosphorylation and degradation

    M Ando, RG Katare, Y Kakinuma, H Yamasaki, T Shishido, C Zheng, MH Li, T Sato

    CIRCULATION110 ( 17 ) 296 - 297   2004年10月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

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  • Vagal nerve stimulation and acetylcholine protect cardiomyocytes from acute ischemia and hypoxia through non-hypoxic induction of hypoxia-inducible factor-1 alpha

    Y Kakinuma, M Kuwabara, Y Doi, M Ando, T Sato

    CIRCULATION110 ( 17 ) 201 - 201   2004年10月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

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  • Clinical application of bionic baroreflex system for automatic control of arterial pressure during surgery

    F Yamasaki, T Ushida, T Yokoyama, K Yamashita, K Sato, M Ando, T Sugiura, T Satou

    CIRCULATION108 ( 17 ) 267 - 268   2003年10月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

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  • 血管条の機能的構造:共焦点レーザー顕微鏡による研究.

    竹内俊二, 安藤元紀, 柿木章伸

    日本耳科学会誌11   84 - 86   2002年

  • QUANTITATIVE POLARIZATION MICROSCOPIC ANALYSIS OF MICROTUBULE DYNAMICS IN A HELIOZOAN ECHINOSPHAERIUM DURING AXOPODIAL CONTRACTION

    T SUZAKI, M ANDO, Y SHIGENAKA

    CELL MOTILITY AND THE CYTOSKELETON23 ( 4 ) 308 - 308   1992年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:WILEY-LISS  

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  • CONTRACTILE MOTILITY AND DYNAMIC CHANGES OF CYTOSKELETON IN HELIOZOAN AXOPODIA

    Y SHIGENAKA, M ANDO

    JOURNAL OF ELECTRON MICROSCOPY38 ( 4 ) 256 - 256   1989年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:JAPANESE SOC ELECTRON MICRO BUS CENTER ACAD SOC JAPAN  

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▼全件表示

講演・口頭発表等

  • Morphological analysis of the cochlear stria vascularis in wild type and melanocyte-deficient <i>Mitf <sup>mi-bw</sup>/Mitf <sup>mi-bw</sup></i> mutant mice.

    第91回日本動物学会大会  2020年9月5日 

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    開催年月日: 2020年9月4日 - 2020年9月5日

    会議種別:口頭発表(一般)  

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  • Three-dimensional relationship between sensory cilia and nervous system in an acoel worm, <i>Praesagittifera naikaiensis</i>.

    第91回日本動物学会大会  2020年9月4日 

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    開催年月日: 2020年9月4日 - 2020年9月5日

    会議種別:口頭発表(一般)  

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  • Development of immunocytochemical procedures suitable for a heliozoan <i>Raphidiophrys contractilis</i> without induction of the rapid axopodial contraction by chemical fixation.

    第90回日本動物学会大会  2019年 

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  • Immunohistochemical identification of inner ear epithelial cells in the tegmentum vasculosum of the quail cochlea.

    第90回日本動物学会大会  2019年 

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  • Novel insights into neural structures during development after hatching in <i>Praesagittifera naikaiensis</i>, an acoel flat worm.

    第90回日本動物学会大会  2019年 

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  • Ultrastructural and immunocytochemical analyses of post-hatching development of an acoelomorph worm, <i>Praesagittifera naikaiensis</i>.

    第89回日本動物学会大会  2018年 

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  • 内耳蝸牛管上皮組織におけるH<sup>+</sup>/<i>myo</i>-inositol transporterおよびaquaporin 4の局在解析.

    第75回岡山実験動物研究会例会  2018年 

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  • Distribution of cytoskeletal elements in an acoelomorph worm, <i>Praesagittifera naikaiensis</i>.

    第88回日本動物学会大会  2017年 

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  • <i>Actinophrys sol</i>の有糸分裂阻害剤に対する薬剤感受性の検討.

    第49回日本原生生物学会大会  2016年 

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  • 日本原生生物学会で発表された研究課題と理科教育との関わり:学習指導要領の理科-生命領域のコンテンツマップを指標として.

    第49回日本原生生物学会大会  2016年 

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    会議種別:シンポジウム・ワークショップ パネル(指名)  

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  • Cellular localization of Kir4.1 and Na<sup>+</sup>-K<sup>+</sup>-ATPase in the tegmentum vasculosum of the avian cochlea.

    22nd Internatinal Congress of Zoology (ICZ)  2016年 

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  • New insights into the mechanism of microtubule shortening during rapid axopodial contraction in heliozoan <i>Polyplacocystis contractilis</i>.

    22nd Internatinal Congress of Zoology (ICZ)  2016年 

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  • 急速な軸足収縮を誘発させずに化学固定したハリタイヨウチュウにおける微小管動態の超微形態学的解析.

    第47回日本原生生物学会大会  2015年 

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  • Expression and localization analysis of Kir4.1 in the tegmentum vasculosum of the avian cochlea.

    第86回日本動物学会大会  2015年 

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  • Ultrastructural analysis using a novel fixation method for preventing the induction of axopodial contraction in <i>Raphidiophrys contractilis</i>.

    第86回日本動物学会大会  2015年 

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  • Immunohistochemical localization of H<sup>+</sup>-coupled myo-inositol transporter (HMIT) and aquaporin in the cochlear lateral wall.

    第85回日本動物学会大会  2014年 

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  • Microtubule dynamics during rapid axopodial contraction in heliozoon <i>Raphidiophrys contractilis</i>.

    第85回日本動物学会大会  2014年 

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  • ハリタイヨウチュウにおける急速な軸足収縮の新規メカニズム.

    第47回日本原生生物学会大会  2014年 

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  • 免疫電子顕微鏡法によるタイヨウチュウRaphidiophrys contractilisの軸足収縮時における微小管動態の解析.

    第46回日本原生動物学会大会  2013年 

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  • Ultrastructural changes during axopodial contraction and re-elongation in heliozoon <i>Raphidiophrys contractilis</sup>.

    第84回日本動物学会大会  2013年 

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  • Identification of unspecified glucose transport gene in the cochlear stria vascularis.

    第84回日本動物学会大会  2013年 

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  • Analysis of tight-junction proteins in the tegmentum vasculosum of the avian cochlea.

    第84回日本動物学会大会  2013年 

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  • ユーグレナの形態変化に伴う誘電挙動の実時間現象論解析.

    第46回日本原生動物学会大会  2013年 

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  • Immunological identification of H+-coupled myo-inositol cotransporter (HMIT) in the isolated marginal cell of the cochlear stria vascularis.

    第84回日本動物学会大会  2013年 

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  • Changes in the localization of cytoskeletal proteins during postnatal development of the mouse cochlear stria vascularis.

    第84回日本動物学会大会  2013年 

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  • Direct evidence of glucose uptake in strial marginal cells of the cochlea: Application of a fluorescent tracer method combined with immunohistochemistry.

    14th International Congress of Histochemistry and Cytochemistry (ICHC)  2012年 

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  • Postnatal expression and localization of glucose transporters in the lateral wall of the cochlear duct.

    日本動物学会第83回大会  2012年 

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  • Identification of epithelial cells in the tegmentum vasculosum of the chicken cochlea by immunofluorescence microscopy.

    日本動物学会第83回大会  2012年 

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  • Assessment of glucose uptake in the cochlear strial marginal cells with a fluorescent tracer method combined with immunohistochemistry.

    日本動物学会第83回大会  2012年 

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  • ハリタイヨウチュウの急速な軸足収縮時における超微構造の変化

    第45回日本原生動物学会大会  2012年 

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  • 海産カイアシ類に寄生する隔口類繊毛虫 Vampyrophrya pelagica の phoront期における微細構造の変化

    第44回日本原生動物学会大会  2011年 

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  • Metamorphosis of the apostome ciliate Vampyrophrya pelagica with dynamic changes of cytoplasmic organelles during host infection.

    8th Internatinal Congress of Comparative Physiology and Biochemistry  2011年 

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  • Immunohistochemical localization of glucose transporters (GLUT5,-10) in the rat cochlea.

    日本動物学会第82回大会  2011年 

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  • Immunohistochemical localization of H+-coupled myo-inositol cotransporter (HMIT) in the rat cochlea.

    日本動物学会第82回大会  2011年 

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  • ハリタイヨウチュウを用いた水質モニタリング装置の試作

    第44回日本原生動物学会大会  2011年 

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  • Immunohistochemical localization of glucose transporter (GLUT1) in the chicken cochlea.

    日本動物学会第82回大会  2011年 

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  • Expression and localization of myo-inositol transporters in the lateral wall of the cochlear duct.

    日本動物学会第81回大会  2010年 

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  • ハリタイヨウチュウを用いた水質モニタリング装置の実用化に向けての課題

    第43回日本原生動物学会大会  2010年 

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  • Lamellar complexes in the trophont of Vampyrophrya pelagica, a histophagous apostome ciliate associated with marine copepods.

    日本動物学会第81回大会  2010年 

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  • タイヨウチュウRaphidiophrys contractilisにおける急速な軸足収縮誘発後の軸足伸長反応

    第43回日本原生動物学会大会  2010年 

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  • A novel bio-monitoring system for aquatic hazards using adhesiveness of the heliozoan cells to substratum.

    日本動物学会第81回大会  2010年 

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  • 葉緑体欠損ミドリムシの誘電挙動.

    第42回日本原生動物学会大会  2009年 

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  • Quantitative analysis of glucose-transporter gene expression in the cochlea stria vascularis.

    日本動物学会第80回大会  2009年 

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  • 内耳蝸牛血管条における膜輸送体分子と毛細血管網の関係.

    第18回日本バイオイメージング学会学術集会  2009年 

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  • タイヨウチュウActinophrys solを利用した抗腫瘍活性を有する微小管阻害剤のin vivoアッセイ系の検討.

    第42回日本原生動物学会大会  2009年 

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  • A novel bio-monitoring system for aquatic toxicants using the heliozoon Raphidiophrys contractilis.

    日本動物学会第80回大会  2009年 

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  • Cell-specific expression of glucose transporter in the cochlear stria vascularis.

    第79回日本動物学会大会  2008年 

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  • Continuous dielectric monitoring for cell shape changes of the flagellate Euglena gracilis.

    第79回日本動物学会大会  2008年 

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  • 連続誘電測定によるミドリムシの細胞動態の解析.

    第41回日本原生動物学会大会  2008年 

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  • タイヨウチュウRaphidiophrys contractilisを用いた水質モニタリングシステムに応用可能な簡易型流動チャンバーの検討

    第41回日本原生動物学会大会  2008年 

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  • 誘電解析法を用いたミドリムシの細胞変形能の解析.

    第40回日本原生動物学会大会  2007年 

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  • Cardiomyocytes produce acetylcholine in response to muscarinic receptor agonists: a possible mechanism for cardioprotective effects of vagal stimulation on cardiomyocytes.

    第71回日本循環器学会総会  2007年 

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  • Donepezil promotes angiogenesis through the mechanisms independent of cholinesterase inhibition and nicotinic alpha7 receptor.

    第71回日本循環器学会総会  2007年 

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  • Electrophysiological approaches to the mechanism of arrhythmogenesis in artificially-arranged cardiomyocytes.

    第71回日本循環器学会総会  2007年 

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  • Cholinesterase inhibitor donepezil improves ventricular function of mice with non-ischemic chronic heart failure.

    第71回日本循環器学会総会  2007年 

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  • Vagal nerve stimulation protects heart against ischemic insult through differential regulation of myocardial TNF receptor subtypes.

    第71回日本循環器学会総会  2007年 

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  • Vagal nerve stimulation salvage the myocardium against reperfusion injury through inhibition of opening of mitochondrial permeability transition pore.

    第71回日本循環器学会総会  2007年 

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  • 不規則チルト負荷試験による圧受容器反射の動特性の推定~マウスからヒトまで

    第57回循環器負荷研究会シンポジウム  2007年 

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  • Nitric oxide has beneficial effects on cardiomyocytes by activation of the vascular endothelial growth factor.

    第71回日本循環器学会総会  2007年 

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  • Dielectric monitoring for cell shape changes in the flagellate Euglena gracilis.

    第78回日本動物学会大会  2007年 

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  • Glucose transport mechanism in the cochlear stria vascularis.

    第78回日本動物学会大会  2007年 

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  • 脊髄刺激による血圧制御

    第59回日本自律神経学会総会シンポジウム  2006年 

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  • 不規則チルト法と平衡線図解析法を用いた圧受容器反射機能の評価

    第59回日本自律神経学会総会シンポジウム  2006年 

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  • Intermittent stretch-induced formation of gap junction between fibrocytes and cardiomyocytes improves dyssynchronous contraction in circular engineered heart tissue.

    Scientific Sessions 2006, American Heart Association  2006年 

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  • Engineered heart tissue - A novel tool for studying the acute ischemia induced changes in-vitro.

    Scientific Sessions 2006, American Heart Association  2006年 

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  • Electrophysiological characterization of artificially-arranged human myocardial cells

    Scientific Sessions 2006, American Heart Association  2006年 

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  • Acetylcholine inhibits mitochondrial permeability transition pore and protects myocardium against acute ischemia-reperfusion injury

    第70回日本循環器学会総会  2006年 

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  • Vagal nerve stimulation differentially regulates TNF receptors and protects the heart against acute ischemic injury

    第70回日本循環器学会総会  2006年 

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  • Acetylcholine inhibits opening of mitochondrial permeability transition pore (PTP) and enhances the functional recovery after long time hypothermic heart preservation

    第70回日本循環器学会総会  2006年 

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  • Activation of the non-neuronal cholinergic system by donepezil, an acetylcholinesterase inhibitor, involves angiogenesis through VEGF production

    第70回日本循環器学会総会  2006年 

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  • Gap junctional communication between cardiomyocyte and fibroblast improves electrical conduction in 3D-engineered remodeled heart

    第70回日本循環器学会総会  2006年 

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  • Vagal nerve stimulation activates TNF-alpha and protects heart against acute ischemic injury

    第69回日本循環器学会総会  2005年 

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  • A HIF-1alpha-regulated gene, a factor involved in anti-apoptosis and cardioprotection against hypoxia through depression of cardiac energy metabolism

    第69回日本循環器学会総会  2005年 

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  • Opposite response of core temperature and blunted response of forearm skin temperature to bicycle ergometer exercise in chronic heart failure

    第69回日本循環器学会総会  2005年 

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  • 動脈圧反射系の平衡線図解析

    第15回日本病態生理学会,細見記念シンポジウム  2005年 

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  • Noninvasive approach for manipulating arterial pressure (AP) using abdominal air shock pants

    第69回日本循環器学会総会  2005年 

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  • 圧受容器反射系の動特性:マウスからヒトまで

    第15回日本病態生理学会  2005年 

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  • 迷走神経刺激とギャップ結合

    第15回日本病態生理学会  2005年 

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  • Neural interface therapy for protecting the heart against ischemia-induced arrhythmia and apoptosis

    第20回生体・生理工学シンポジウム(ニューロエンジニアリング)  2005年 

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  • Vagal nerve stimulation differentially regulates TNF receptors and protect the heart against acute ischemic injury

    Scientific Sessions 2005, American Heart Association  2005年 

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  • Granulocyte colony-stimulating factor activates cell survival signaling cascade to protect cardiomyocyte from cell death

    第69回日本循環器学会総会  2005年 

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  • Acetylcholine from vagal nerve stimulation protects cardiomyocytes against acute ischemia and hypoxia by additive induction of hypoxia-inducible factor-1alpha through nonhypoxic pathway

    第69回日本循環器学会総会  2005年 

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  • Donepezil, acetylcholinesterase inhibitor, promotes angiogenesis over infarct area in rats after chronic coronary occlusion

    Scientific Sessions 2005, American Heart Association  2005年 

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  • Acetylcholine regulates a HIF-1alpha-mediated gene, involved in cardiac energy metabolism suppression and cardioprotection against hypoxia

    Scientific Sessions 2005, American Heart Association  2005年 

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  • Different roles of vagal and sympathetic systems in heart rate control for stabilizing arterial pressure (AP) against orthostatic stress

    第68回日本循環器学会総会  2004年 

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  • Opposite response of core temperature (Tcore) and blunted response of forearm skin temperature (Tskin) to bicycle ergometer exercise in chronic heart failure (CHF)

    第68回日本循環器学会総会  2004年 

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  • Clinical application of bionic baroreflex systen (BBS) for automatic control of arterial pressure (AP)

    第68回日本循環器学会総会  2004年 

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  • Vagal nerve stimulation and acetylcholine protect cardiomyocytes from acute ischemia and hypoxia through non-hypoxic induction of hypoxia-inducible factor-1alpha

    Scientific Sessions 2004, American Heart Association  2004年 

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  • Vagal Stimulation Suppresses Ischemia-Induced Arrhythmias by Preserving Connexin43 Protein from Its Dephosphorylation and Degradation

    Scientific Sessions 2004, American Heart Association  2004年 

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  • 迷走神経刺激は急性心筋虚血によるギャップ結合タンパク質コネキシン43のリン酸化タイプの減少を防ぐ

    第25回日本循環制御医学会総会  2004年 

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  • 迷走神経刺激とコネキシン

    第5回Neurocardiology Workshop  2004年 

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  • Analytic and integrative framework for human sympathetic baroreflex control: equilibrium diagram of arterial pressure (AP) and plasma norepinephrine level (PNE)

    第67回日本循環器学会総会  2003年 

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  • New potential interface for revitalization of baroreflex function: epidural catheter approach in humans

    第67回日本循環器学会総会  2003年 

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  • Integrative and open-loop approach for estimation of human baroreflex dynamics

    第67回日本循環器学会総会  2003年 

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  • Baroreflex dynamics in mice and rats: white-noise system identification during random head-up tilting

    第80回日本生理学会大会  2003年 

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  • Dynamic modulation of cardiac booster effect: transfer function analysis from central venous pressure (CVP) to systemic arterial pressure (SAP)

    第67回日本循環器学会総会  2003年 

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  • 圧反射系の動特性を評価するあたらしい枠組み

    第24回日本循環制御医学会総会  2003年 

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  • 心臓の圧ブースター効果:中心静脈圧から体循環動脈圧への伝達特性

    第24回日本循環制御医学会総会  2003年 

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  • Estimation of human baroreflex dynamics comparing between normal and baroreflex failure

    第67回日本循環器学会総会  2003年 

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  • マウス動脈圧受容器反射の動特性の推定

    第54回日本生理学会中国四国地方会  2002年 

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  • Effect of perilymphatic Cs+ on the endocochlear potential

    24th Association for Research in Otolaryngology  2001年 

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  • Expression of mRNA encoding 'ClC-K1', a kidney Cl--channel in marginal cells of the stria vascularis or rat cochlea

    第78回日本生理学会大会  2001年 

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  • 内耳血管条の微小循環システムにおける虚血の影響

    第53回日本生理学会中国四国地方会  2001年 

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  • Bumetanide-induced enlargement of the intercellular space in the stria vascularis is not dependent on perilymphatic K+

    24th Association for Research in Otolaryngology  2001年 

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  • Effects of perilymphatic Cs+ on the endocochlear potential

    第78回日本生理学会大会  2001年 

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  • モルモット血管条内のグルコース単輸送体(GLUT1):単離細胞を用いた局在の検討

    第10回日本耳科学会  2000年 

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  • Expression of ClC-K1 Cl- channel m-RNA in the rat stria vascularis: its possible relation to the whole-cell Cl currents in marginal cells

    23rd Association for Research in Otolaryngology  2000年 

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  • モルモット血管条中間細胞の膜電位とEPの関連:Ba2+とCs+に対する感受性の比較

    第10回日本耳科学会  2000年 

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  • 内向き整流性K+チャネル(Kir4.1)血管条中間細胞への局在

    第9回日本耳科学会  1999年 

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  • Elongated pericyte-like cells connect discrete capillaries in the stria vascularis

    22nd Association for Research in Otolaryngology  1999年 

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  • A voltage-activated outward K+ current in the intermediate cell of the stria vascularis

    22nd Association for Research in Otolaryngology  1999年 

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  • An inwardly rectifying K+ conductance in intermediate cells and a nonselective cation conductance in marginal cells

    21st Association for Research in Otolaryngology  1998年 

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  • Dye-coupling of intermediate cells with endothelial cells, pericytes and basal cells in the stria vascularis of gerbils

    21st Association for Research in Otolaryngology  1998年 

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  • 中間細胞の内向き整流性K+コンダクタンスと外向き遅延整流性K+コンダクタンス

    第8回日本耳科学会  1997年 

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  • Dielectric dispersion of protein bound water

    第74回日本生理学会大会  1997年 

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  • Dielectric monitoring of the severity of pulmonary edema induced in the rat lung

    XXXIII International Congress of Physiological Sciences  1997年 

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  • 辺縁細胞のグルコース単輸送体(GLUT):ミトコンドリア内NADHの自家蛍光を利用した生理活性の証明

    第7回日本耳科学会  1997年 

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  • 蝸牛管側壁の毛細血管網の発達過程:蛍光ゼラチン-共焦点レーザー顕微鏡による3次元観察

    第7回日本耳科学会  1997年 

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  • 血管条における血管周皮細胞の3次元分布:蛍光免疫染色した基底膜を指標とした解析

    第8回日本耳科学会  1997年 

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  • Dielectric dispersion analysis of erythrocyte rouleau formation

    第73回日本生理学会大会  1996年 

     詳細を見る

  • Usefulness of dielectric spectroscopy in quantitative evaluation of rat cold cataract

    第73回日本生理学会大会  1996年 

     詳細を見る

  • Dielectric dispersion analysis of isolated bovine chromaffin granules and ghost

    第71回日本生理学会大会  1995年 

     詳細を見る

  • 赤血球連銭形成時の誘電挙動

    第47回日本生理学会中四国地方会  1995年 

     詳細を見る

  • Dielectric analysis of the rat thyroid gland by modeling the follicle as a key structure

    第70回日本生理学会大会  1994年 

     詳細を見る

  • Dielectric spectroscopic monitoring of the formation of lung edema by use of a surface electrode

    第70回日本生理学会大会  1994年 

     詳細を見る

  • Dielectric dispersion analysis of the rat thyroid gland

    XXXII International Congress of Physiological Sciences  1993年 

     詳細を見る

  • 家兎外傷性前極白内障にともなう水晶体誘電挙動の変化

    第97回日本眼科学会総会  1993年 

     詳細を見る

  • Dielectric behavior of rat submandibular gland; simulation with a vesicle-inclusion cell model

    XXXII International Congress of Physiological Sciences  1993年 

     詳細を見る

  • ユーグレナ運動に伴う細胞インピーダンスの変化

    第25回日本原生動物学会大会  1992年 

     詳細を見る

  • Healing process of injured corneas as monitored by non-invasive dielectric spectroscopy

    第69回日本生理学会大会  1992年 

     詳細を見る

  • Dielectric measurements of the rabbit lens in vivo using a surface probe

    第69回日本生理学会大会  1992年 

     詳細を見る

  • Dielectric dispersion analysis of isolated chromaffin granules at low ionic strength

    第69回日本生理学会大会  1992年 

     詳細を見る

  • Dielectric behavior of rat submandibular glands and its simulation with a spherical-shell model

    第69回日本生理学会大会  1992年 

     詳細を見る

  • 家兎水晶体の誘電挙動-眼球表面からのアプローチ

    第96回日本眼科学会総会  1992年 

     詳細を見る

  • 家兎角膜の誘電挙動-角膜上皮の創傷治癒過程

    第96回日本眼科学会総会  1992年 

     詳細を見る

  • 誘電分散法による肺水腫評価法の基礎的検討

    第44回日本胸部外科学会学術集会  1991年 

     詳細を見る

  • Obserbation of axonemal microtubules in a heliozoan Echinosphaerium by polarization microscopy

    日本動物学会中国四国地方会  1991年 

     詳細を見る

  • Dielectric behavior of excised rat lung lobes in the 10^2-10^8 Hz range as correlated with pulmonary edema

    第68回日本生理学会大会  1991年 

     詳細を見る

  • 白色家兎角膜10 kHz-100 MHz領域の誘電測定

    第95回日本眼科学会総会  1991年 

     詳細を見る

  • 肺水腫形成時における誘電挙動の変化

    第43回日本生理学会中四国地方会  1991年 

     詳細を見る

  • Dielectric dispersion analysis of isolated chromaffin granules

    第44回日本細胞生物学会大会  1991年 

     詳細を見る

  • 水晶体in vivo誘電測定への取り組み

    第30回日本白内障学会  1991年 

     詳細を見る

  • 単離された分泌顆粒の形態計測-副腎クロマフィン顆粒の誘電解析に関連して

    第42回日本生理学会中四国地方会  1990年 

     詳細を見る

  • 太陽虫軸足の収縮運動と細胞骨格の動的変化

    第45回日本電子顕微鏡学会・学術講演会  1989年 

     詳細を見る

  • Quantitative polarization microscopical analysis of axonemal microtubules in a heliozoan Echinosphaerium during axopodial contraction

    第43回日本細胞生物学会大会  1989年 

     詳細を見る

  • Mechanism of rapid axopodial contraction in Echinospaerium

    VIII International Congress of Protozoology  1989年 

     詳細を見る

  • The dynamic change of cytoskeleton in the helioozoan axopodium during rapid axopodial contraction

    第42回日本細胞生物学会大会  1989年 

     詳細を見る

  • Mechanism of axopodial contraction in heliozoa

    第41回日本細胞生物学会大会  1988年 

     詳細を見る

▼全件表示

受賞

  • ひらめき☆ときめきサイエンス推進賞

    2016年  

     詳細を見る

    受賞国:日本国

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  • 日本バイオイメージング学会 ベストイメージ・カールツァイス賞

    2009年  

     詳細を見る

    受賞国:日本国

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  • 日本循環制御医学会 会長賞

    2004年  

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    受賞国:日本国

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  • 日本循環制御医学会 ベストプレゼンテーション賞

    2003年  

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    受賞国:日本国

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共同研究・競争的資金等の研究

  • 新しい細胞運動・細胞骨格系の探索とその応用技術の開発

      詳細を見る

    資金種別:競争的資金

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  • 循環動態を解析する基盤技術の開発

      詳細を見る

    資金種別:競争的資金

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  • 内耳蝸牛のイオン輸送機構と糖輸送機構の解明

      詳細を見る

    資金種別:競争的資金

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  • 生体インピーダンス測定による非侵襲的組織解析法の開発

      詳細を見る

    資金種別:競争的資金

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担当授業科目

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  • 学問の方法 (2020年度) 第1学期  - 火1,火2

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