Updated on 2025/10/23

写真a

 
ANDO Motonori
 
Organization
Faculty of Education Professor
Position
Professor
External link

Degree

  • 博士(医学)

Research Interests

  • 自然科学教育

  • 聴覚

  • 細胞骨格

  • Cell physiology

  • 細胞運動

  • 循環

  • 膜輸送

  • 環境応答

Research Areas

  • Life Science / Clinical pharmacy

  • Life Science / Anatomy

  • Life Science / Animal physiological chemistry, physiology and behavioral biology

  • Life Science / Otorhinolaryngology

  • Life Science / Physiology

Research History

  • Okayama University   Graduate School of Education   Professor

    2012

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  • - Professor,Graduate School of Education,Okayama University

    2012

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  • Okayama University   Graduate School of Education   Associate Professor

    2005 - 2012

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  • Associate Professor,Graduate School of Education,Okayama University

    2005 - 2012

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  • Assistant Professor,Kochi Medical School

    1990 - 2005

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  • Kochi University   Medical School   Assistant Professor

    1990 - 2005

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Professional Memberships

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Papers

  • Notes on present status and future prospects of web-based scanning electron microscopy.

    Motonori Ando, Chiyu Nakano, Tosuke Sakagami, Kanako Hayashi

    Journal of Electron Microscopy Technology for Medicine and Biology   36   60 - 61   2024.2

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  • Structure of putative epidermal sensory receptors in an acoel flatworm, Praesagittifera naikaiensis. Reviewed

    Tosuke Sakagami, Kaho Watanabe, Mayuko Hamada, Tatsuya Sakamoto, Toshimitsu Hatabu, Motonori Ando

    Cell and Tissue Research   395   299 - 311   2024.1

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    DOI: 10.1007/s00441-024-03865-y

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  • Morphological analysis of the cochlear stria vascularis in wild type and melanocyte-deficient Mitf mi-bw/Mitf mi-bw mutant mice.

    Proceedings of Okayama Association for Laboratory Animal Science   36   3 - 9   2023.12

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  • De novo transcriptome analysis of the centrohelid Raphidocystis contractilis to identify genes involved in microtubule-based motility. Reviewed

    Risa Ikeda, Tosuke Sakagami, Mayuko Hamada, Tatsuya Sakamoto, Toshimitsu Hatabu, Noboru Saito, Motonori Ando

    Journal of Eukaryotic Microbiology   e12955   2022.11

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/jeu.12955

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  • Structural analysis of the statocyst and nervous system of Praesagittifera naikaiensis, an acoel flatworm, during development after hatching. Reviewed

    Tosuke Sakagami, Kaho Watanabe, Risa Ikeda, Motonori Ando

    Zoomorphology   140 ( 2 )   183 - 192   2021.4

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00435-021-00521-9

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    Other Link: http://link.springer.com/article/10.1007/s00435-021-00521-9/fulltext.html

  • Immunocytochemical analysis of α-tubulin distribution before and after rapid axopodial contraction in the centrohelid Raphidocystis contractilis. Reviewed

    Risa Ikeda, Miki Kurokawa, Momoka Murai, Noboru Saito, Motonori Ando

    Acta Protozoologica   59 ( 1 )   1 - 12   2020.4

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Uniwersytet Jagiellonski - Wydawnictwo Uniwersytetu Jagiellonskiego  

    DOI: 10.4467/16890027ap.20.001.12157

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  • Differential localizations of the myo-inositol transporters HMIT and SMIT1 in the cochlear stria vascularis. Reviewed

    Midori Edamatsu, Yasuhiro Kondo, Motonori Ando

    Neuroscience Letters   674   88 - 93   2018.5

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    DOI: 10.1016/j.neulet.2018.03.028

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  • Cytoskeletal elements in an acoelomorph worm, Praesagittifera naikaiensis.

    Risa Ikeda, Chiho Fujiwara, Mayuko Hamada, Tatsuya Sakamoto, Noboru Saito, Motonori Ando

    Proceedings of Okayama Association for Laboratory Animal Science   34   21 - 27   2018

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  • Structure and function of tegmentum vasculosum in avian cochlea.

    Risa Ikeda, Tsuyoshi Otono, Naoya Ikeda, Noboru Saito, Motonori Ando

    Proceedings of Okayama Association for Laboratory Animal Science   33   26 - 30   2017

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  • Direct evidence of the glucose uptake into cochlear strial marginal cells: Application of a fluorescent tracer method combined with immunohistochemistry. Reviewed

    Sohta Hishikawa, Midori Edamatsu, Risa Inoue-Ikeda, Motonori Ando

    Bioimages   23   1 - 8   2016

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Bioimaging Society  

    Despite the importance of glucose as an energy source for marginal cells of the cochlear stria vascularis, no direct evidence of the glucose uptake into these cells has been demonstrated. In this study, we investigated the glucose pathway in strial marginal cells using a fluorescent tracer method combined with immunohistochemistry. For glucose imaging, a non-metabolizable fluorescent glucose analog, 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (6-NBDG), was intravenously injected into mice. After 6-NBDG fluorescent signals in cochlear sections were observed, the same sections were processed for immunohistochemistry to identify marginal cells. This showed that 6-NBDG fluorescent signals had passed into the cytosol of marginal cells via Na+/K+-ATPase-positive basolateral membranes. These results provide direct physiological evidence of the glucose uptake into epithelial strial marginal cells. This combined method can help in examining the relationship between target molecules and functional proteins using the same tissue samples.

    DOI: 10.11169/bioimages.23.1

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  • Acetylcholine Suppresses Ventricular Arrhythmias and Improves Conduction and Connexin-43 Properties During Myocardial Ischemia in Isolated Rabbit Hearts. Reviewed

    Takeshi Aiba, Takashi Noda, Ichiro Hidaka, Masashi Inagaki, Rajesh G. Katare, Motonori Ando, Kenji Sunagawa, Takayuki Sato, Masaru Sugimachi

    Journal of Cardiovascular Electrophysiology   26 ( 6 )   678 - 685   2015.6

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    DOI: 10.1111/jce.12663

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  • Immunological identification of H+-coupled myo-inositol cotransporter in the lateral wall of the cochlear duct.

    Mayumi Yamaji, Risa Inoue, Midori Edamatsu, Motonori Ando

    Proceedings of Okayama Association for Laboratory Animal Science   31   55 - 58   2015

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  • Microtubule dynamics during rapid axopodial contraction in heliozoon Raphidiophrys contractilis revealed by immunoelectron microscopy.

    Risa Inoue, Motonori Ando

    Japanese Journal of Protozoology   47   3   2014

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  • Real-time phenomenological analysis of dielectric behavior of Euglena cells during their cell-shape changes.

    Ken Hanahara, Motonori Ando

    Japanese Journal of Protozoology   47   4   2014

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  • Physiological significance of intercellular communication mediated by tissue-specific membrane transport systems:insights from heart and inner-ear tissues.

    Motonori Ando

    Proceedings of Okayama Association for Laboratory Animal Science   29   55 - 60   2013

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  • Ultrastructural changes during rapid axopodial contraction in heliozoon Raphidiophrys contractilis.

    Risa Inoue, Midori Edamatsu, Motonori Ando

    Japanese Journal of Protozoology   46   3   2013

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  • Three-dimensional observation of the vascular networks and functional proteins in the cochlear stria vascularis using a non-corroded casting method combined with an immunohistochemical analysis. Reviewed

    Midori Edamatsu, Sohta Hishikawa, Yasuhiro Kondo, Motonori Ando

    Bioimages   20   9 - 15   2012

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    DOI: 10.11169/bioimages.20.9

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  • Ultrastructural metamorphosis of the apostome ciliate Vampyrophrya pelagica in the phoront stage during and after infection to pelagic copepods.

    Atsushi Kanazawa, Toshinobu Suzaki, Motonori Ando, Susumu Ohtsuka

    Japanese Journal of Protozoology   45   21   2012

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  • A prototype of the biomonitoring system for assessing water quality using heliozoon cells.

    Chisato Yoshimura, Motonori Ando, Toshinobu Suzaki

    Japanese Journal of Protozoology   45   33   2012

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  • Multiple expression of glucose transporters in the lateral wall of the cochlear duct studied by quantitative real-time PCR assay Reviewed

    Midori Edamatsu, Yasuhiro Kondo, Motonori Ando

    Neuroscience Letters   490 ( 1 )   72 - 77   2011.2

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    DOI: 10.1016/j.neulet.2010.12.029

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  • Re-elongation of axopodia after induction of rapid axopodial contraction in heliozoon Raphidiophrys contractilis.

    Toshie Enomoto, Toshinobu Suzaki, Motonori Ando

    Japanese Journal of Protozoology   44   58 - 59   2011

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  • Steps toward the practical use of a system for water quality monitoring using heliozoon cells.

    Chisato Yoshimura, Motonori Ando, Toshinobu Suzaki

    Japanese Journal of Protozoology   44   53 - 54   2011

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  • Differential regulation of TNF receptors by vagal nerve stimulation protects heart against acute ischemic injury Reviewed

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Mikihiko Arikawa, Fumiyasu Yamasaki, Takayuki Sato

    Journal of Molecular and Cellular Cardiology   49 ( 2 )   234 - 244   2010.8

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    DOI: 10.1016/j.yjmcc.2010.03.007

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  • Engineered Heart Tissue: A Novel Tool to Study the Ischemic Changes of the Heart In Vitro. Reviewed

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Takayuki Sato

    PLOS ONE   5 ( 2 )   1 - 5   2010.2

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    DOI: 10.1371/journal.pone.0009275

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  • Dielectric behavior of the flagellate Euglena gracilis SM-ZK, a permanent chloroplast-lacking mutant.

    Sho Fukuizumi, Toshinobu Suzaki, Motonori Ando

    Japanese Journal of Protozoology   43   41 - 42   2010

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  • Evaluation of an in-vivo bioassay system for microtubule inhibitors with antitumor activity using the heliozoon Actinophrys sol.

    Toshie Enomoto, Teruhiko Ishikawa, Toshinobu Suzaki, Motonori Ando

    Japanese Journal of Protozoology   43   66 - 67   2010

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  • 聴覚機能におけるエネルギー供給システムの解明

    安藤元紀

    生物学に関する試験研究論叢   25   74 - 78   2010

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  • Anti-Alzheimer's Drug, Donepezil, Markedly Improves Long-Term Survival After Chronic Heart Failure in Mice. Reviewed

    Takemi Handa, Rajesh G. Katare, Yoshihiko Kakinuma, Mikihiko Arikawa, Motonori Ando, Shiro Sasaguri, Fumiyasu Yamasaki, Takayuki Sato

    Journal of Cardiac Failure   15 ( 9 )   805 - 811   2009.11

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    DOI: 10.1016/j.cardfail.2009.05.008

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  • Vagal nerve stimulation prevents reperfusion injury through inhibition of opening of mitochondrial permeability transition pore independent of the bradycardiac effect. Reviewed

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Mikihiko Arikawa, Takemi Handa, Fumiyasu Yamasaki, Takayuki Sato

    Journal of Thoracic and Cardiovascular Surgery   137 ( 1 )   223 - 231   2009.1

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    DOI: 10.1016/j.jtcvs.2008.08.020

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  • Continuous dielectric measurement system for monitoring cell-shape dynamics in the flagellate Euglena gracilis.

    Sho Fukuizumi, Toshie Enomoto, Midori Edamatsu, Toshinobe Suzaki, Motonori Ando

    Japanese Journal of Protozoology   42   74   2009

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  • Improvement of a flow-through type chamber for the aquatic bio-monitoring system using adhesiveness of heliozoon cells to substratum.

    Toshie Enomoto, Sho Fukuizumi, Midori Edamatsu, Toshinobu Suzaki, Motonori Ando

    Japanese Journal of Protozoology   42   22 - 23   2009

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  • Donepezil, an acetylcholinesterase inhibitor for treatment of Alzheimer’s disease, improves left ventricular function and survival in volume-overload heart failure mice Reviewed

    Takemi Handa, Rajesh G. Katare, Yoshihiko Kakinuma, Mikihiko Arikawa, Motonori Ando, Fumiyasu Yamasaki, Takayuki Sato, Shiro Sasaguri

    Shinzo   41 ( 2 )   115 - 123   2009

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    Language:Japanese   Publisher:Japan Heart Foundation  

    DOI: 10.11281/shinzo.41.115

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    Other Link: http://search.jamas.or.jp/link/ui/2009128118

  • Cellular localization of facilitated glucose transporter 1 (GLUT-1) in the cochlear stria vascularis: its possible contribution to the transcellular glucose pathway Reviewed

    Motonori Ando, Midori Edamatsu, Sho Fukuizumi, Shunji Takeuchi

    Cell and Tissue Research   331 ( 3 )   763 - 769   2008.3

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    DOI: 10.1007/s00441-007-0495-2

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  • Dielectric analysis for cell shape changes in the flagellate Euglena gracilis.

    Sho Fukuizumi, Midori Edamatsu, Toshinobu Suzaki, Motonori Ando

    Japanese Journal of Protozoology   41 ( 1 )   55 - 57   2008

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  • 不規則チルト負荷試験による圧受容器反射の動特性の推定~マウスからヒトまで Reviewed

    安藤元紀, 山崎文靖, 佐藤隆幸

    心臓   40 ( 2 )   34 - 36   2008

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    Authorship:Lead author   Language:Japanese   Publisher:公益財団法人 日本心臓財団  

    DOI: 10.11281/shinzo1969.40.Supplement2_34

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    Other Link: http://search.jamas.or.jp/link/ui/2008283508

  • Progression of ventricular dysfunction after myocardial infarction is prevented by Anti-Alzheimer's disease drug, donepezil. Reviewed

    Mikihiko Arikawa, Rajesh G. Katare, Yoshihiko Kakinuma, Handa Takemi, Motonori Ando, Fumiyasu Yamasaki, Takayuki Sato

    Circulation   116 ( 16 )   291   2007.10

  • Granulocyte colony-stimulating factor activates Wnt signal to sustain gap junction function through recruitment of beta-catenin and cadherin Reviewed

    Masanorl Kuwabara, Yoshihiko Kakinuma, Rajesh G. Katare, Motonori Ando, Fumiyasu Yamasaki, Yoshinori Doi, Takayuki Sato

    FEBS Letters   581 ( 25 )   4821 - 4830   2007.10

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    DOI: 10.1016/j.febslet.2007.09.007

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  • 脊髄刺激による血圧制御 Reviewed

    山崎文靖, 牛田享宏, 横山武志, 山下幸一, 安藤元紀, 佐藤隆幸

    自律神経   44   236 - 242   2007

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  • Novel analytic frameworks for identifying human baroreflex dynamics: random head-up tilting and equilibrium diagram analysis. Reviewed

    Ando M, Yamasaki F, Sato T

    Autonomic Nervous System (Tokyo)   44   229 - 235   2007

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  • Electrophysiological characterization of artificially-arranged human myocardial cells. Reviewed

    Mikihiko Arikawa, Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Fumiyasu Yamasaki, Mercy M. Davidson, Takayuki Sato

    Circulation   114 ( 18 )   291   2006.10

  • Vagal nerve stimulation attenuates myocardial ischemia - Reperfusion injury by inhibiting the mitochondrial permeability transition pore. Reviewed

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Mikihiko Arikawa, Takemi Handa, Fumiyasu Yamasaki, Shiro Sasaguri, Takayuki Sato

    Circulation   114 ( 18 )   1196   2006.10

  • Engineered heart tissue - A novel tool for studying the acute ischemia induced changes in-vitro. Reviewed

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Mikihiko Arikawa, Fumiyasu Yamasaki, Takayuki Sato

    Circulation   114 ( 18 )   12   2006.10

  • Intermittent stretch-induced formation of gap junction between fibrocytes and cardiomyocytes improves dyssynchronous contraction circular engineered heart tissue. Reviewed

    Motonori Ando, Rajesh G. Katare, Yoshihiko Kakinuma, Fumiyasu Yamasaki, Takayuki Sato

    CirculationI   114 ( 18 )   80   2006.10

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  • Acetylcholine inhibits the hypoxia-induced reduction of connexin43 protein in rat cardiomyocytes. Reviewed

    Yanan Zhang, Yoshihiko Kakinuma, Motonori Ando, Rajesh G. Katare, Fumiyasu Yamasaki, Tetsuro Sugiura, Takayuki Sato

    Journal of Pharmacological Sciences   101 ( 3 )   214 - 222   2006.7

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    DOI: 10.1254/jphs.FP0051023

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  • Nitric oxide stimulates vascular endothelial growth factor production in cardiomyocytes involved in angiogenesis. Reviewed

    Masanori Kuwabara, Yoshihiko Kakinuma, Motonori Ando, Rajesh G. Katare, Fumiyasu Yamasaki, Yoshinori Doi, Takayuki Sato

    Journal of Physiological Sciences   56 ( 1 )   95 - 101   2006.2

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    DOI: 10.2170/physiolsci.RP002305

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  • Artificial baroreflex - Clinical application of a bionic baroreflex system. Reviewed

    Fumiyasu Yamasaki, Takahiro Ushida, Takeshi Yokoyama, Motonori Ando, Koichi Yamashita, Takayuki Sato

    Circulation   113 ( 5 )   634 - 639   2006.2

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    DOI: 10.1161/CIRCULATIONAHA.105.587915

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  • 神経インターフェイス技法による血圧制御と心不全治療 Reviewed

    佐藤 隆幸, 山崎 文靖, 安藤 元紀

    人工臓器   34 ( 2 )   "S - 40"   2005.11

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    Language:Japanese   Publisher:JAPANESE SOCIETY FOR ARTIFICIAL ORGANS  

    DOI: 10.11392/jsao1972.34.2Supplement_s40

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    Other Link: http://search.jamas.or.jp/link/ui/2006076512

  • Donepezil, acetylcholinesterase inhibitor, promotes angiogenesis over infarct area in rats after chronic coronary occlusion. Reviewed

    Motonori Ando, Rajesh G. Katare, Yoshihiko Kakinuma, Meihua Li, Can Zheng, Fumiyasu Yamasaki, Takayuki Sato

    Circulation   112 ( 17 )   U342 - U342   2005.10

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  • Vagal nerve stimulation differentially regulates TNF receptors and protect the heart against acute ischemic injury. Reviewed

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Fumiyasu Yamasaki, Takayuki Sato

    Circulation   112 ( 17 )   U251 - U252   2005.10

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  • Efferent vagal nerve stimulation protects heart against ischemia-induced arrhythmias by preserving connexin43 protein. Reviewed

    Motonori Ando, Rajesh G. Katare, Yoshihiko Kakinuma, Dongmei Zhang, Fumiyasu Yamasaki, Kazuyo Muramoto, Takayuki Sato

    Circulation   112 ( 2 )   164 - 170   2005.7

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    DOI: 10.1161/CIRCULATIONAHA.104.525493

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  • Acetylcholine from vagal stimulation protects cardiomyocytes against ischemia and hypoxia involving additive non-hypoxic induction of HIF-1 alpha. Reviewed

    Yoshihiko Kakinuma, Motonori Ando, Masanori Kuwabara, Rajesh G. Katare, Koji Okudela, Masanobu Kobayashi, Takayuki Sato

    FEBS Letters   579 ( 10 )   2111 - 2118   2005.4

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    DOI: 10.1016/j.febslet.2005.02.065

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  • Dielectric behavior of pulmonary edema induced in the rat lung. Reviewed

    Toshiyuki Yamashiro, Motonori Ando, Yasunaga Okazaki, Shiro Sasaguri

    Respiratory Physiology & Neurobiology   145 ( 1 )   91 - 100   2005.1

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    DOI: 10.1016/j.resp.2004.08.008

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  • Neural interface therapy for protecting the heart against ischemia-induced arrhythmia and apoptosis.

    Motonori Ando, Rajesh G. Katare, Yoshihiko Kakinuma, Takayuki Sato

    Proceedings of the Symposium on Biological and Physiological Engineering   20   51 - 54   2005

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  • Effect of electrical modification of cardiornyocytes on transcriptional activity through 5 '-AMP-activated protein kinase. Reviewed

    Yoshihiko Kakinuma, Yanan Zhang, Motonori Ando, Tetsuro Sugiura, Takayuki Sato

    Journal of Cardiovascular Pharmacology   44   S435 - S438   2004.11

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    DOI: 10.1097/01.fjc.0000166318.91623.f9

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  • Vagal nerve stimulation and acetylcholine protect cardiomyocytes from acute ischemia and hypoxia through non-hypoxic induction of hypoxia-inducible factor-1 alpha. Reviewed

    Yoshikiko Kakinuma, Masanori Kuwabara, Yoshinori Doi, Motonori Ando, Takayuki Sato

    Circulation   110 ( 17 )   201 - 201   2004.10

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  • Vagal stimulation suppresses ischemia-induced arrhythmias by preserving connexin43 protein from dephosphorylation and degradation. Reviewed

    Motonori Ando, Rajesh G. Katare, Yoshihiko Kakinuma, Fumiyasu Yamasaki, Toshiaki Shishido, Can Zheng, Meihua Li, Takayuki Sato

    Circulation   110 ( 17 )   296 - 297   2004.10

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  • Carotid-sinus baroreflex modulation of core and skin temperatures in rats: An open-loop approach. Reviewed

    Dongmei Zhang, Motonori Ando, Fumiyasu Yamasaki, Takayuki Sato

    Japanese Journal of Physiology   53 ( 6 )   461 - 466   2003.12

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    DOI: 10.2170/jjphysiol.53.461

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  • Clinical application of bionic baroreflex system for automatic control of arterial pressure during surgery Reviewed

    Fumiyasu Yamasaki, Takahiro Ushida, Takeshi Yokoyama, Koichi Yamashita, Kyoko Sato, Motonori Ando, Tetsuro Sugiura, Takayuki Sato

    Circulation   108 ( 17 )   267 - 268   2003.10

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  • Aquaporin-1 (AQP1) is expressed in the stria vascularis of rat cochlea. Reviewed

    Shoichi Sawada, Taizo Takeda, Hiroaki Kitano, Shunji Takeuchi, Teruhiko Okada, Motonori Ando, Mikio Suzuki, Akinobu Kakigi

    Hearing Research   181 ( 1-2 )   15 - 19   2003.7

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    DOI: 10.1016/S0378-5955(03)00131-X

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  • Acute ischemia causes 'dark cell' change of strial marginal cells in gerbil cochlea Reviewed

    Motonori Ando, Shunji Takeuchi, Akinobu Kakigi, Valerică Raicu, Ken-ichi Yagyu, Takayuki Sato

    CELL AND TISSUE RESEARCH   309 ( 2 )   229 - 235   2002.8

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    DOI: 10.1007/s00441-002-0597-9

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  • Reduction in the endocochlear potential caused by Cs+ in the perilymph can be explained by the five-compartment model of the stria vascularis. Reviewed

    Akinobu Kakigi, Shunji Takeuchi, Motonori Ando, Kasumi Higashiyama, Hiroshi Azuma, Takayuki Sato, Taizo Takeda

    Hearing Research   166 ( 1-2 )   54 - 61   2002.4

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    DOI: 10.1016/S0378-5955(01)00412-9

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  • Bumetanide-induced enlargement of the intercellular space in the stria vascularis requires an active Na+-K+-ATPase. Reviewed

    Hiroshi Azuma, Shunji Takeuchi, Kasumi Higashiyama, Motonori Ando, Akinobu Kakigi, Mitsuhiko Nakahira, Kazuhiro Yamakawa, Taizo Takeda

    Acta Oto-Laryngologica   122 ( 8 )   816 - 821   2002

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    DOI: 10.1080/003655402/000028051

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  • Functional structure of the stria vascularis studied by confocal laser microscopy. Reviewed

    Takeuchi S, Ando M, Kakigi A

    Otology Japan   11 ( 2 )   84 - 86   2002

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    The lateral wall of the cochlear duct dissected from gerbils or rats was studied by confocal laser microscopy. Specific structures in tissue preparations were stained with fluorescent dyes to clarify the following points: i) developmental changes in the capillary network in the lateral wall of the cochlear duct, ii) intercellular connection by gap junctions, iii) distribution of elongated cells connecting capillaries, and iv) three dimensional distribution of intermediate cells in the stria vascularis. These studies revealed structural characteristics related to specific functions of the stria vascularis.

    DOI: 10.11289/otoljpn1991.11.84

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  • Three-dimensional and ultrastructural relationships between intermediate cells and capillaries in the gerbil stria vascularis. Reviewed

    Shunji Takeuchi, Motonori Ando, Takayuki Sato, Akinobu Kakigi

    Hearing Research   155 ( 1-2 )   103 - 112   2001.5

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    DOI: 10.1016/S0378-5955(01)00252-0

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  • Mechanism generating endocochlear potential: Role played by intermediate cells in stria vascularis. Reviewed

    Shunji Takeuchi, Motonori Ando, Akinobu Kakigi

    Biophysical Journal   79 ( 5 )   2572 - 2582   2000.11

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    DOI: 10.1016/S0006-3495(00)76497-6

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  • mRNA encoding 'CIC-K1, a kidney Cl--channel' is expressed in marginal cells of the stria vascularis of rat cochlea: its possible contribution to Cl- currents. Reviewed

    Motonori Ando, Shunji Takeuchi

    Neuroscience Letters   284 ( 3 )   171 - 174   2000.4

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    DOI: 10.1016/S0304-3940(00)01021-1

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  • Immunological identification of an inward rectifier K+ channel (Kir4.1) in the intermediate cell (melanocyte) of the cochlear stria vascularis of gerbils and rats. Reviewed

    Motonori Ando, Shunji Takeuchi

    Cell and Tissue Research   298 ( 1 )   179 - 183   1999.10

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    DOI: 10.1007/s004419900066

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  • Voltage-dependent outward K+ current in intermediate cell of stria vascularis of gerbil cochlea. Reviewed

    Shunji Takeuchi, Motonori Ando

    American Journal of Physiology-Cell Physiology   277 ( 1 )   C91 - C99   1999.7

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    DOI: 10.1152/ajpcell.1999.277.1.C91

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  • Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats. Reviewed

    Motonori Ando, Akinobu Kakigi, Shunji Takeuchi

    Cell and Tissue Research   296 ( 3 )   673 - 676   1999.6

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    DOI: 10.1007/s004410051327

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  • Postnatal vascular development in the lateral wall of the cochlear duct of gerbils: quantitative analysis by electron microscopy and confocal laser microscopy Reviewed

    Motonori Ando, Shunji Takeuchi

    Hearing RESEARCH   123 ( 1-2 )   148 - 156   1998.9

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    DOI: 10.1016/S0378-5955(98)00109-9

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  • Dye-coupling of melanocytes with endothelial cells and pericytes in the cochlea of gerbils. Reviewed

    Shunji Takeuchi, Motonori Ando

    Cell and Tissue Research   293 ( 2 )   271 - 275   1998.8

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    DOI: 10.1007/s004410051118

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  • Inwardly rectifying K+ currents in intermediate cells in the cochlea of gerbils: a possible contribution to the endocochlear potential. Reviewed

    Shunji Takeuchi, Motonori Ando

    Neuroscience Letters   247 ( 2-3 )   175 - 178   1998.5

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    DOI: 10.1016/S0304-3940(98)00318-8

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  • Marginal cells of the stria vascularis of gerbils take up glucose via the facilitated transporter GLUT: application of autofluorescence. Reviewed

    Shunji Takeuchi, Motonori Ando

    Hearing Research   114 ( 1-2 )   69 - 74   1997.12

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    DOI: 10.1016/S0378-5955(97)00157-3

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  • Changes in the volume of marginal cells induced by isotonic 'Cl- depletion/restoration': involvement of the Cl- channel and Na+-K+-Cl- cotransporter. Reviewed

    Shunji Takeuchi, Motonori Ando, Akihiko Irimajiri

    Hearing Research   113 ( 1-2 )   99 - 109   1997.11

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    DOI: 10.1016/S0378-5955(97)00134-2

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  • Dielectric monitoring of rouleaux formation in human whole blood: A feasibility study Reviewed

    Akihiko Irimajiri, Motonori Ando, Rika Matsuoka, Takako Ichinowatari, Shunji Takeuchi

    Biochimica et Biophysica Acta-General Subjects   1290 ( 3 )   207 - 209   1996.8

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    DOI: 10.1016/0304-4165(96)00048-7

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  • Ion channels in basolateral membrane of marginal cells dissociated from gerbil stria vascularis. Reviewed

    Shunji Takeuchi, Motonori Ando, Kenichi Kozakura, Haruo Saito, Akihiko Irimajiri

    Hearing Research   83 ( 1-2 )   89 - 100   1995.3

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    DOI: 10.1016/0378-5955(94)00191-R

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  • Structure and function of the cytoskeleton in Heliozoa: 3. Rapid microtubule disorganization during axopodial contraction in Echinosphaerium. Reviewed

    Toshinobu Suzaki, Motonori Ando, Yoko Inai, Yoshinobu Shigenaka

    European Journal of Protistology   30 ( 4 )   404 - 413   1994.11

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    DOI: 10.1016/S0932-4739(11)80215-4

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  • Dielectric behavior of the rabbit cornea as a measure of the healing process in injured epithelium. Reviewed

    Yasuko Mokudai, Makio Watanabe, Motonori Ando, Hisayuki Ueno

    Journal of Japanese Ophthalmological Society   98 ( 3 )   215 - 223   1994

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  • Dielectric measurements on the rabbit cornea using a surface electrode. Reviewed

    Makio Watanabe, Yasuko Mokudai, Hisayuki Ueno, Motonori Ando, Akihiko Irimajiri

    Journal of Japanese Ophthalmological Society   97 ( 5 )   569 - 574   1993

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  • Structure and function of the cytoskeleton in Heliozoa: 2. Measurement of the force of rapid axopodial contraction in Echinosphaerium. Reviewed

    Toshinobu Suzaki, Motonori Ando, Katsuyoshi Ishigame, Yoshinobu Shigenaka, Masanori Sugiyama

    European Journal of Protistology   28 ( 4 )   430 - 433   1992.11

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    DOI: 10.1016/S0932-4739(11)80007-6

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  • A computer program for estimating size distribution of spherical organelles from electron micrographs.

    Toshinobu Suzaki, Motonori Ando

    Memoirs of the Faculty of Integrated Arts and Sciences, Hiroshima University, Ser.IV   18   35 - 42   1992

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    Size distributions of intracellular organelles in an ultra-thin section are usually distorted when the section thickness is not negligible. A computer program is developed here to estimate true size distributions of spherical organelles from transmission electron micrographs of ultra-thin sections. The method is applied to determine size distributions of chromaffin granules isolated from bovine adrenal medullary cells as a basis for further physiological measurements.

    DOI: 10.15027/622

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    Other Link: http://ir.lib.hiroshima-u.ac.jp/00000622

  • Quantitative polarization microscopic analysis of microtubule dynamics in a heliozoan Echinosphaerium during axopodial contraction. Reviewed

    Suzaki T, Ando M, Shigenaka Y

    Cell Motility and the Cytoskeleton   23 ( 4 )   308 - 308   1992

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  • Structure and function of the cytoskeleton in Heliozoa: 1. Mechanism of rapid axopodial contraction in Echinosphaerium. Reviewed

    Motonori Ando, Yoshinobu Shigenaka

    Cell Motility and the Cytoskeleton   14 ( 2 )   288 - 301   1989

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    DOI: 10.1002/cm.970140214

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  • Ultrastructure of the isolated microtubules and intermicrotubular bridges in a heterotrichous ciliate, Spirostomum ambiguum. Reviewed

    Shigenaka Yoshinobu, Jun Hosoi, Motonori Ando, Masaki Ishida, Izumi Ishii

    Journal of Electron Microscopy   38 ( 5 )   363 - 370   1989

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    DOI: 10.1093/oxfordjournals.jmicro.a050750

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  • Contractile motility and dynamic changes of cytoskeleton in heliozoan axopodia. Reviewed

    Shigenaka Y, Ando M

    Journal of Electron Microsocpy   38 ( 4 )   256 - 256   1989

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  • Contractility of heliozoan axopodia.

    Motonori Ando, Yoshinobu Shigenaka

    Japanese Journal of Protozoology   22   34 - 35   1988

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Presentations

  • Cellular localization of solute carrier 26 like genes expressed in the acoel Praesagittifera naikaiensis.

    Yuna Nakamura, Kanako Hayashi, Tosuke Sakagami, Motonori Ando

    2025.9.5 

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    Event date: 2025.9.4 - 2025.9.6

    Presentation type:Poster presentation  

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  • Regenerative wound healing in the acoel Praesagittifera naikaiensis with loss of tail from below the mouth organ.

    Wataru Shiraishi, Kanako Hayashi, Yuna Nakamura, Tosuke Sakagami, Motonori Ando

    2025.9.5 

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    Event date: 2025.9.4 - 2025.9.6

    Presentation type:Poster presentation  

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  • Spatial arrangement of green algae in the acoel Praesagittifera naikaiensis.

    Kanako Hayashi, Shiho Nakagawa, Yuna Nakamura, Tosuke Sakagami, Motonori Ando

    2025.9.5 

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    Event date: 2025.9.4 - 2025.9.6

    Presentation type:Poster presentation  

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  • 原生生物太陽虫が示す普遍的な生命現象と系統進化

    池田理佐, 林加奈子, 黒川美樹, 安藤元紀

    第109回日本生物教育学会大会  2025.3.16 

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    Event date: 2025.3.15 - 2025.3.16

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  • 腎機能を理解するための教材開発とその実践

    池田理佐, 田中福人, 安藤元紀

    第108回日本生物教育学会大会  2024.1.6 

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    Event date: 2024.1.6 - 2024.1.8

    Presentation type:Oral presentation (general)  

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  • 遠隔操作による走査型電子顕微鏡観察の現状と今後の展望 Invited

    安藤元紀, 中野知佑, 坂上登亮, 林加奈子

    第39回医学生物学電子顕微鏡技術学会学術講演会  2023.9.17 

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    Event date: 2023.9.16 - 2023.9.17

    Presentation type:Symposium, workshop panel (nominated)  

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  • Novel insights into the establishment of the acoel-algal symbiosis.

    Hayashi K, Sakagami T, Ando M

    2023.9.9 

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    Event date: 2023.9.7 - 2023.9.9

    Presentation type:Oral presentation (general)  

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  • Expression and localization of the facilitated glucose transporters (GLUT1, 3) in the mouse epididymal duct.

    Masui K, Sakagami T, Ando M

    2023.9.9 

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    Event date: 2023.9.7 - 2023.9.9

    Presentation type:Oral presentation (general)  

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  • Structure and function of epidermal sensory receptors in an acoel flatworm, Praesagittifera naikaiensis.

    Sakagami T, Hamada M, Hatabu T, Ando M

    2023.9.9 

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    Event date: 2023.9.7 - 2023.9.9

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  • Site-specific expression of the novel sugar transporter in mouse epididymis.

    Koichiro Masui, Tosuke Sakagami, Naho Kanda, Kanako Hayashi, Motonori Ando

    2022.9.10 

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    Event date: 2022.9.8 - 2022.9.10

    Presentation type:Oral presentation (general)  

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  • Novel insights into structure and function of the sensory organs in an acoel worm, Praesagittifera naikaiensis.

    Tosuke Sakagami, Kaho Watanabe, Mayuko Hamada, Toshimitsu Hatabu, Motonori Ando

    2021.9.2 

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    Event date: 2021.9.1 - 2021.9.4

    Presentation type:Oral presentation (general)  

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  • Expression and localization of sugar transporters in mouse epididymis.

    Naho Kanda, Haruya Kobayashi, Takumi Omi, Tosuke Sakagami, Motonori Ando

    2021.9.2 

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    Event date: 2021.9.1 - 2021.9.4

    Presentation type:Oral presentation (general)  

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  • Microtubule dynamics before and after rapid axopodial contraction in the heliozoan Raphidocystis contractilis.

    Miki Kurokawa, Tosuke Sakagami, Risa Ikeda, Motonori Ando

    2021.9.2 

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    Event date: 2021.9.1 - 2021.9.4

    Presentation type:Oral presentation (general)  

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  • Immunocytochemical and ultrastructural analysis of axopodia in the heliozoan Actinophrys sol.

    Rin Ogawa, Miki Kurokawa, Tosuke Sakagami, Motonori Ando

    2021.9.2 

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    Event date: 2021.9.1 - 2021.9.4

    Presentation type:Oral presentation (general)  

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  • Morphological analysis of the cochlear stria vascularis in wild type and melanocyte-deficient Mitf mi-bw/Mitf mi-bw mutant mice.

    Mari Jigyo, Naho Kanda, Hayura Kobayashi, Tosuke Sakagami, Hiroaki Yamamoto, Motonori Ando

    2020.9.5 

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    Event date: 2020.9.4 - 2020.9.5

    Presentation type:Oral presentation (general)  

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  • Three-dimensional relationship between sensory cilia and nervous system in an acoel worm, Praesagittifera naikaiensis.

    Kaho Watanabe, Tosuke Sakagami, Miki Kurokawa, Disuke Fukuoka, Motonori Ando

    2020.9.4 

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    Event date: 2020.9.4 - 2020.9.5

    Presentation type:Oral presentation (general)  

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  • 圧受容器反射異常の基礎から治療 脊髄刺激による血圧制御

    山崎 文靖, 牛田 亨宏, 横山 武志, 山下 幸一, 安藤 元紀, 佐藤 隆幸

    第59回日本自律神経学会総会  2006.11  日本自律神経学会

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    Language:Japanese  

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  • Structure-function relationships in unicellular and multicellular organisms: insights from macro and micro perspectives. Invited

    Motonori Ando

    2024.12.7 

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  • Effect of different culture conditions on the photosymbiotic relationship between an acoel Praesagittifera naikaiensis and Tetraselmis sp.

    Kanako Hayashi, Yuna Nakamura, Shiho Nakagawa, Tosuke Sakagami, Motonori Ando

    2024.9.12 

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  • Solute carrier 26 like genes expressed in an acoel flatworm, Praesagittifera naikaiensis.

    Yuna Nakamura, Kanako Hayashi, Wataru Shiraishi, Tosuke Sakagami, Motonori Ando

    2024.9.12 

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  • マウス精巣上体に発現する糖輸送体の網羅的な解析

    桝井光一郎, 坂上登亮, 安藤元紀

    第80回岡山実験動物研究会例会  2023.9.1 

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  • Development of immunocytochemical procedures suitable for a heliozoan Raphidiophrys contractilis without induction of the rapid axopodial contraction by chemical fixation.

    Risa Ikeda, Momoka Murai, Miki Kurokawa, Tosuke Sakagami, Noboru Saito, Motonori Ando

    2019 

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  • Immunohistochemical identification of inner ear epithelial cells in the tegmentum vasculosum of the quail cochlea.

    Naho Kanda N, Haruya Kobayashi, Tosuke Sakagami, Risa Ikeda, Noboru Saito, Motonori Ando

    2019 

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  • Novel insights into neural structures during development after hatching in Praesagittifera naikaiensis, an acoel flat worm.

    Tosuke Sakagami, Kaho Watanabe, Risa Ikeda, Motonori Ando

    2019 

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  • 内耳蝸牛管上皮組織におけるH+/myo-inositol transporterおよびaquaporin 4の局在解析

    小林春也, 宮本歩実, 池田理佐, 安藤元紀

    第75回岡山実験動物研究会例会  2018 

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  • Ultrastructural and immunocytochemical analyses of post-hatching development of an acoelomorph worm, Praesagittifera naikaiensis.

    Risa Ikeda, Tosuke Sakagami, Junpei Kaneda, Motonori Ando

    2018 

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  • Distribution of cytoskeletal elements in an acoelomorph worm, Praesagittifera naikaiensis.

    Chiho Fujiwara, Risa Ikeda, Mayuko Hamada, Tatsuya Sakamoto, Motonori Ando

    2017 

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  • 日本原生生物学会で発表された研究課題と理科教育との関わり:学習指導要領の理科-生命領域のコンテンツマップを指標として

    池田理佐, 安藤元紀

    第49回日本原生生物学会大会  2016 

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  • Cellular localization of Kir4.1 and Na+-K+-ATPase in the tegmentum vasculosum of the avian cochlea.

    Takae Nose, Tsuyoshi Otono, Risa Ikeda, Motonori Ando

    2016 

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  • New insights into the mechanism of microtubule shortening during rapid axopodial contraction in heliozoan Polyplacocystis contractilis.

    Risa Ikeda, Shogo Matsui, Noboru Saito, Motonori Ando

    2016 

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  • Actinophrys solの有糸分裂阻害剤に対する薬剤感受性の検討

    松井祥悟, 池田理佐, 安藤元紀

    第49回日本原生生物学会大会  2016 

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  • Expression and localization analysis of Kir4.1 in the tegmentum vasculosum of the avian cochlea.

    Tsuyoshi Otono, Risa Inoue, Motonori Ando

    2015 

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  • Ultrastructural analysis using a novel fixation method for preventing the induction of axopodial contraction in Raphidiophrys contractilis.

    Risa Inoue, Noboru Saito, Motonori Ando

    2015 

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  • 急速な軸足収縮を誘発させずに化学固定したハリタイヨウチュウにおける微小管動態の超微形態学的解析

    井上理佐, 村田和義, 齋藤昇, 安藤元紀

    第47回日本原生生物学会大会  2015 

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  • Microtubule dynamics during rapid axopodial contraction in heliozoon Raphidiophrys contractilis.

    Risa Inoue, Motonori Ando

    2014 

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  • ハリタイヨウチュウにおける急速な軸足収縮の新規メカニズム

    井上理佐, 安藤元紀

    第47回日本原生生物学会大会  2014 

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  • Immunohistochemical localization of H+-coupled myo-inositol transporter (HMIT) and aquaporin in the cochlear lateral wall.

    Mayumi Yamaji, Midori Edamatsu, Motonori Ando

    2014 

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  • 免疫電子顕微鏡法によるタイヨウチュウRaphidiophrys contractilisの軸足収縮時における微小管動態の解析

    井上理佐, 安藤元紀

    第46回日本原生動物学会大会  2013 

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  • Ultrastructural changes during axopodial contraction and re-elongation in heliozoon Raphidiophrys contractilis.

    Risa Inoue, Motonori Ando

    2013 

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  • Identification of unspecified glucose transport gene in the cochlear stria vascularis.

    Kayo Nagasaka, Motonori Ando

    2013 

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  • Analysis of tight-junction proteins in the tegmentum vasculosum of the avian cochlea.

    Shinnosuke Higuchi, Motonori Ando

    2013 

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  • ユーグレナの形態変化に伴う誘電挙動の実時間現象論解析

    花原健, 安藤元紀

    第46回日本原生動物学会大会  2013 

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  • Immunological identification of H+-coupled <ital>myo</ital>-inositol cotransporter (HMIT) in the isolated marginal cell of the cochlear stria vascularis.

    Mayumi Yamaji, Midori Edamatsu, Motonori Ando

    2013 

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  • Changes in the localization of cytoskeletal proteins during postnatal development of the mouse cochlear stria vascularis.

    Yuko Goto, Shinnosuke Higuchi, Motonori Ando

    2013 

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  • Identification of epithelial cells in the tegmentum vasculosum of the chicken cochlea by immunofluorescence microscopy.

    Shinnosuke Higuchi, Midori Edamatsu, Yasuhiro Kondo, Motonori Ando

    2012 

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  • Assessment of glucose uptake in the cochlear strial marginal cells with a fluorescent tracer method combined with immunohistochemistry.

    Sohta Hishikawa, Midori Edamatsu, Motonori Ando

    2012 

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  • ハリタイヨウチュウの急速な軸足収縮時における超微構造の変化

    井上理佐, 枝松緑, 安藤元紀

    第45回日本原生動物学会大会  2012 

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  • Direct evidence of glucose uptake in strial marginal cells of the cochlea: Application of a fluorescent tracer method combined with immunohistochemistry.

    Sohta Hishikawa, Midori Edamatsu, Motonori Ando

    14th International Congress of Histochemistry and Cytochemistry (ICHC)  2012 

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  • Postnatal expression and localization of glucose transporters in the lateral wall of the cochlear duct.

    Naoya Ikeda, Sohta Hishikawa, Midori Edamatsu, Motonori Ando

    2012 

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  • Immunohistochemical localization of myo-inositol transporters, HMIT and SMIT1, in the lateral wall of the rat cochlea.

    Midori Edamatsu, Yasuhiro Kondo, Motonori Ando

    14th International Congress of Histochemistry and Cytochemistry (ICHC)  2012 

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  • ハリタイヨウチュウを用いた水質モニタリング装置の試作

    吉村知里, 安藤元紀, 洲崎敏伸

    第44回日本原生動物学会大会  2011 

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  • Immunohistochemical localization of glucose transporter (GLUT1) in the chicken cochlea.

    Shinnosuke Higuchi, Midori Edamatsu, Yasuhiro Kondo, Motonori Ando

    2011 

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  • 海産カイアシ類に寄生する隔口類繊毛虫 Vampyrophrya pelagica の phoront期における微細構造の変化

    金澤篤志, 洲崎敏伸, 安藤元紀, 大塚 攻

    第44回日本原生動物学会大会  2011 

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  • Immunohistochemical localization of H+-coupled myo-inositol cotransporter (HMIT) in the rat cochlea.

    Midori Edamatsu, Kaori Haruna, Yasuhiro Kondo, Motonori Ando

    2011 

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  • Metamorphosis of the apostome ciliate Vampyrophrya pelagica with dynamic changes of cytoplasmic organelles during host infection.

    Atsushi Kanazawa, Toshinobu Suzaki, Motonori Ando, Susumu Ohtsuka

    8th Internatinal Congress of Comparative Physiology and Biochemistry  2011 

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  • Immunohistochemical localization of glucose transporters (GLUT5,-10) in the rat cochlea.

    Sohta Hishikawa, Midori Edamatsu, Motonori Ando

    2011 

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  • Expression and localization of myo-inositol transporters in the lateral wall of the cochlear duct.

    Midori Edamatsu, Erika Date, Naoya Ikeda, Yasuhiro Kondo, Motonori Ando

    2010 

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  • ハリタイヨウチュウを用いた水質モニタリング装置の実用化に向けての課題

    吉村知里, 安藤元紀, 洲崎敏伸

    第43回日本原生動物学会大会  2010 

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  • Lamellar complexes in the trophont of Vampyrophrya pelagica, a histophagous apostome ciliate associated with marine copepods.

    Atsushi Kanazawa, Susumu Ohtsuka, Toshinobu Suzaki, Motonori Ando

    2010 

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  • タイヨウチュウRaphidiophrys contractilisにおける急速な軸足収縮誘発後の軸足伸長反応

    榎本淑恵, 洲崎敏伸, 安藤元紀

    第43回日本原生動物学会大会  2010 

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  • A novel bio-monitoring system for aquatic hazards using adhesiveness of the heliozoan cells to substratum.

    Motonori Ando, Toshinobu Suzaki

    2010 

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  • 葉緑体欠損ミドリムシの誘電挙動

    福泉 翔, 洲崎敏伸, 安藤元紀

    第42回日本原生動物学会大会  2009 

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  • Quantitative analysis of glucose-transporter gene expression in the cochlea stria vascularis.

    Midori Edamatsu, Toshie Enomoto, Motonori Ando

    2009 

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  • 内耳蝸牛血管条における膜輸送体分子と毛細血管網の関係

    枝松 緑, 安藤元紀

    第18回日本バイオイメージング学会学術集会  2009 

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  • タイヨウチュウActinophrys solを利用した抗腫瘍活性を有する微小管阻害剤のin vivoアッセイ系の検討

    榎本淑恵, 石川彰彦, 洲崎敏伸, 安藤元紀

    第42回日本原生動物学会大会  2009 

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  • A novel bio-monitoring system for aquatic toxicants using the heliozoon Raphidiophrys contractilis.

    Toshie Enomoto, Midori Edamatsu, Toshinobu Suzaki, Motonori Ando

    2009 

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  • Cell-specific expression of glucose transporter in the cochlear stria vascularis.

    2008 

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  • Continuous dielectric monitoring for cell shape changes of the flagellate Euglena gracilis.

    Sho Fukuizumi, Midori Edamatsu, Toshinobu Suzaki, Motonori Ando

    2008 

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  • 連続誘電測定によるミドリムシの細胞動態の解析

    福泉 翔, 榎本淑恵, 枝松 緑, 洲崎敏伸, 安藤元紀

    第41回日本原生動物学会大会  2008 

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  • タイヨウチュウRaphidiophrys contractilisを用いた水質モニタリングシステムに応用可能な簡易型流動チャンバーの検討

    榎本淑恵, 福泉 翔, 枝松 緑, 洲崎敏伸, 安藤元紀

    第41回日本原生動物学会大会  2008 

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  • 誘電解析法を用いたミドリムシの細胞変形能の解析

    福泉 翔, 枝松 緑, 洲崎敏伸, 安藤元紀

    第40回日本原生動物学会大会  2007 

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  • Cardiomyocytes produce acetylcholine in response to muscarinic receptor agonists: a possible mechanism for cardioprotective effects of vagal stimulation on cardiomyocytes.

    2007 

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  • Donepezil promotes angiogenesis through the mechanisms independent of cholinesterase inhibition and nicotinic alpha7 receptor.

    Yoshihiko Kakinuma, Rajesh G. Katare, Mikihiko Arikawa, Takemi Handa, Motonori Ando, Meihua Li, Can Zheng, Fumiyasu Yamasaki, Takayuki Sato

    2007 

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  • Electrophysiological approaches to the mechanism of arrhythmogenesis in artificially-arranged cardiomyocytes.

    Mikihiko Arikawa, Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Takemi Handa, Fumiyasu Yamasaki, Mercy M. Davidson, Takayuki Sato

    2007 

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  • Cholinesterase inhibitor donepezil improves ventricular function of mice with non-ischemic chronic heart failure.

    Takemi Handa, Rajesh G. Katare, Yoshihiko Kakinuma, Mikihiko Arikawa, Motonori Ando, Shiro Sasaguri, Takayuki Sato

    2007 

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  • Vagal nerve stimulation protects heart against ischemic insult through differential regulation of myocardial TNF receptor subtypes.

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Mikihiko Arikawa, Takemi Handa, Fumiyasu Yamasaki, Takayuki Sato

    2007 

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  • Vagal nerve stimulation salvage the myocardium against reperfusion injury through inhibition of opening of mitochondrial permeability transition pore.

    Rajesh G. Katare, Yoshihiko Kakinuma, Motonori Ando, Mikihiko Arikawa, Takemi Handa, Fumiyasu Yamasaki, Shiro Sasaguri, Takayuki Sato

    2007 

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  • 不規則チルト負荷試験による圧受容器反射の動特性の推定~マウスからヒトまで

    安藤元紀, 山崎文靖, 佐藤隆幸

    第57回循環器負荷研究会シンポジウム  2007 

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  • Nitric oxide has beneficial effects on cardiomyocytes by activation of the vascular endothelial growth factor.

    Masanori Kuwabara, Yoshihiko Kakinuma, Motonori Ando, Rajesh G. Katare, Fumiyasu Yamasaki, Yoshinori Doi, Takayuki Sato T

    2007 

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  • Dielectric monitoring for cell shape changes in the flagellate Euglena gracilis.

    Sho Fukuizumi, Midori Edamatsu, Toshinobu Suzaki, Motonori Ando

    2007 

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  • Glucose transport mechanism in the cochlear stria vascularis.

    Midori Edamatsu, Sho Fukuizumi, Motonori Ando

    2007 

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  • Acetylcholine inhibits mitochondrial permeability transition pore and protects myocardium against acute ischemia-reperfusion injury.

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Masanori Kuwabara, Fumiyasu Yamasaki, Shiro Sasaguri, Takayuki Sato

    2006 

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  • Vagal nerve stimulation differentially regulates TNF receptors and protects the heart against acute ischemic injury.

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Masanori Kuwabara, Fumiyasu Yamasaki, Takayuki Sato

    2006 

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  • Electrophysiological characterization of artificially-arranged human myocardial cells.

    Mikihiko Arikawa, Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Fumiyasu Yamasaki, Mercy M. Davidson, Takayuki Sato

    2006 

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  • Activation of the non-neuronal cholinergic system by donepezil, an acetylcholinesterase inhibitor, involves angiogenesis through VEGF production.

    Yoshihiko Kakinuma, Motonori Ando, Rajesh G. Katare, Masanori Kuwabara, Fumiyasu Yamasaki, Takayuki Sato

    2006 

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  • Gap junctional communication between cardiomyocyte and fibroblast improves electrical conduction in 3D-engineered remodeled heart.

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Masanori Kuwabara, Fumiyasu Yamasaki, Shiro Sasaguri, Takayuki Sato

    2006 

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  • Acetylcholine inhibits opening of mitochondrial permeability transition pore (PTP) and enhances the functional recovery after long time hypothermic heart preservation.

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Masanori Kuwabara, Fumiyasu Yamasaki, Takayuki Sato

    2006 

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  • 脊髄刺激による血圧制御

    山崎文靖, 牛田亨宏, 横山武志, 山下幸一, 安藤元紀, 佐藤隆幸

    第59回日本自律神経学会総会シンポジウム  2006 

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  • 不規則チルト法と平衡線図解析法を用いた圧受容器反射機能の評価

    安藤元紀, 山崎文靖, 佐藤隆幸

    第59回日本自律神経学会総会シンポジウム  2006 

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  • Intermittent stretch-induced formation of gap junction between fibrocytes and cardiomyocytes improves dyssynchronous contraction in circular engineered heart tissue.

    Motonori Ando, Rajesh G. Katare, Yoshihiko Kakinuma, Fumiyasu Yamasaki, Takayuki Sato

    2006 

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  • Engineered heart tissue - A novel tool for studying the acute ischemia induced changes in-vitro.

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Mikihiko Arikawa, Fumiyasu Yamasaki, Takayuki Sato

    2006 

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  • Vagal nerve stimulation activates TNF-alpha and protects heart against acute ischemic injury.

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Fumiyasu Yamasaki, Takayuki Sato

    2005 

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  • A HIF-1alpha-regulated gene, a factor involved in anti-apoptosis and cardioprotection against hypoxia through depression of cardiac energy metabolism.

    Yoshihiko Kakinuma, Motonori Ando, Masanori Kuwabara, Rajesh G. Katare, Fumiyasu Yamasaki, Takayuki Sato

    2005 

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  • Opposite response of core temperature and blunted response of forearm skin temperature to bicycle ergometer exercise in chronic heart failure.

    Takayuki Sato, Fumiyasu Yamasaki, Motonori Ando, Hiroaki Kitaoka, Jun Takata, Tetsuro Sugiura, Yoshinori Doi

    2005 

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  • 動脈圧反射系の平衡線図解析

    佐藤隆幸, 安藤元紀, 川田 徹

    第15回日本病態生理学会,細見記念シンポジウム  2005 

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  • Noninvasive approach for manipulating arterial pressure (AP) using abdominal air shock pants.

    Fumiyasu Yamasaki, Kyoko Sato, Motonori Ando, Yoshinori Doi, Tetsuro Sugiura, Takayuki Sato

    2005 

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  • 圧受容器反射系の動特性:マウスからヒトまで

    安藤元紀, 佐藤隆幸

    第15回日本病態生理学会  2005 

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  • 迷走神経刺激とギャップ結合

    安藤元紀, 佐藤隆幸

    第15回日本病態生理学会  2005 

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  • Neural interface therapy for protecting the heart against ischemia-induced arrhythmia and apoptosis.

    Motonori Ando, Rajesh G. Katare, Yoshihiko Kakinuma, Takayuki Sato

    2005 

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  • Vagal nerve stimulation differentially regulates TNF receptors and protect the heart against acute ischemic injury.

    Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma, Fumiyasu Yamasaki, Takayuki Sato

    2005 

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  • Granulocyte colony-stimulating factor activates cell survival signaling cascade to protect cardiomyocyte from cell death.

    Masanori Kuwabara, Yoshihiko Kakinuma, Motonori Ando, Rajesh G. Katare, Fumiyasu Yamasaki, Yoshinori Doi Y, Takayuki Sato

    2005 

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  • Acetylcholine from vagal nerve stimulation protects cardiomyocytes against acute ischemia and hypoxia by additive induction of hypoxia-inducible factor-1alpha through nonhypoxic pathway.

    Yoshihiko Kakinuma, Motonori Ando, Masanori Kuwabara, Rajesh G. Katare, Fumiyasu Yamasaki, Takayuki Sato

    2005 

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  • Donepezil, acetylcholinesterase inhibitor, promotes angiogenesis over infarct area in rats after chronic coronary occlusion.

    Motonori Ando, Rajesh G. Katare, Yoshihiko Kakinuma, Meihua Li, Can Zheng, Fumiyasu Yamasaki, Takayuki Sato

    2005 

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  • Acetylcholine regulates a HIF-1alpha-mediated gene, involved in cardiac energy metabolism suppression and cardioprotection against hypoxia.

    Yoshihiko Kakinuma, Motonori Ando, Rajesh G. Katare, Fumiyasu Yamasaki, Takayuki Sato

    2005 

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  • Vagal nerve stimulation and acetylcholine protect cardiomyocytes from acute ischemia and hypoxia through non-hypoxic induction of hypoxia-inducible factor-1alpha.

    Yoshikiko Kakinuma, Masanori Kuwabara, Yoshinori Doi, Motonori Ando, Takayuki Sato

    2004 

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  • Vagal stimulation suppresses ischemia-induced arrhythmias by preserving connexin43 protein from dephosphorylation and degradation.

    Motonori Ando, Rajesh G. Katare, Yoshihiko Kakinuma, Fumiyasu Yamasaki, Toshiaki Shishido, Can Zheng, Meihua Li, Takayuki Sato

    2004 

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  • 迷走神経刺激は急性心筋虚血によるギャップ結合タンパク質コネキシン43のリン酸化タイプの減少を防ぐ

    安藤元紀, 柿沼由彦, カタレ ラジェシュ, 山崎文靖, 佐藤隆幸

    第25回日本循環制御医学会総会  2004 

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  • 迷走神経刺激とコネキシン

    安藤元紀, 柿沼由彦, カタレ ラジェシュ, 山崎文靖, 佐藤隆幸

    第5回Neurocardiology Workshop  2004 

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  • Clinical application of bionic baroreflex systen (BBS) for automatic control of arterial pressure (AP).

    Fumiyasu Yamasaki, Takahiro Ushida, Takeshi Yokoyama, Koichi Yamashita, Kyoko Sato, Motonori Ando, Yoshinori Doi, Tetsuro Sugiura, Takayuki Sato

    2004 

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  • Different roles of vagal and sympathetic systems in heart rate control for stabilizing arterial pressure (AP) against orthostatic stress.

    Fumiyasu Yamasaki, Kyoko Sato, Motonori Ando, Yoshinori Doi, Tetsuro Sugiura, Takayuki Sato

    2004 

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  • Opposite response of core temperature (Tcore) and blunted response of forearm skin temperature (Tskin) to bicycle ergometer exercise in chronic heart failure (CHF).

    Kyoko Sato, Fumiyasu Yamasaki, Motonori Ando, Hiroaki Kitaoka, Jun Takata, Tetsuro Sugiura, Takayuki Sato, Yoshinori Doi

    2004 

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  • Analytic and integrative framework for human sympathetic baroreflex control: equilibrium diagram of arterial pressure (AP) and plasma norepinephrine level (PNE).

    Fumiyasu Yamasaki, Kyoko Sato, Motonori Ando, Yoshinori Doi, Tetsuro Sugiura, Takayuki Sato

    2003 

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  • New potential interface for revitalization of baroreflex function: epidural catheter approach in humans.

    Fumiyasu Yamasaki, Kyoko Sato, Motonori Ando, Takayuki Sato

    2003 

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  • Integrative and open-loop approach for estimation of human baroreflex dynamics.

    Fumiyasu Yamasaki, Kyoko Sato, Motonori Ando, Yoshinori Doi, Tetsuro Sugiura, Takayuki Sato

    2003 

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  • Baroreflex dynamics in mice and rats: white-noise system identification during random head-up tilting.

    Motonori Ando, Fumiyasu Yamasaki, Jun Yuto, Takayuki Sato

    2003 

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  • Dynamic modulation of cardiac booster effect: transfer function analysis from central venous pressure (CVP) to systemic arterial pressure (SAP).

    Motonori Ando, Fumiyasu Yamasaki, Takayuki Sato

    2003 

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  • 圧反射系の動特性を評価するあたらしい枠組み

    安藤元紀, 山崎文靖, 湯藤潤, 佐藤隆幸

    第24回日本循環制御医学会総会  2003 

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  • 心臓の圧ブースター効果:中心静脈圧から体循環動脈圧への伝達特性

    安藤元紀, 山崎文靖, 湯藤潤, 佐藤隆幸

    第24回日本循環制御医学会総会  2003 

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  • Estimation of human baroreflex dynamics comparing between normal and baroreflex failure.

    Fumiyasu Yamasaki, Kyoko Sato, Motonori Ando, Yoshinori Doi, Tetsuro Sugiura, Takayuki Sato

    2003 

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  • マウス動脈圧受容器反射の動特性の推定

    安藤元紀, 湯藤潤, 佐藤隆幸

    第54回日本生理学会中国四国地方会  2002 

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  • Effect of perilymphatic Cs+ on the endocochlear potential.

    Akinobu Kakigi, Motonori Ando, Shunji Takeuchi

    2001 

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  • Expression of mRNA encoding 'ClC-K1', a kidney Cl--channel in marginal cells of the stria vascularis or rat cochlea.

    Motonori Ando, Shunji Takeuchi, Takayuki Sato

    2001 

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  • 内耳血管条の微小循環システムにおける虚血の影響

    安藤元紀, 佐藤隆幸

    第53回日本生理学会中国四国地方会  2001 

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  • Bumetanide-induced enlargement of the intercellular space in the stria vascularis is not dependent on perilymphatic K+.

    Motonori Ando, Akinobu Kakigi, Haruo Saito, Shunji Takeuchi

    2001 

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  • Effects of perilymphatic Cs+ on the endocochlear potential.

    Shunji Takeuchi, Motonori Ando, Akinobu Kakigi

    2001 

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  • モルモット血管条内のグルコース単輸送体(GLUT1):単離細胞を用いた局在の検討

    安藤元紀, 竹内俊二

    第10回日本耳科学会  2000 

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  • Expression of ClC-K1 Cl- channel m-RNA in the rat stria vascularis: its possible relation to the whole-cell Cl currents in marginal cells.

    Motonori Ando, Shunji Takeuchi

    2000 

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  • モルモット血管条中間細胞の膜電位とEPの関連:Ba2+とCs+に対する感受性の比較

    2000 

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  • 内向き整流性K+チャネル(Kir4.1)血管条中間細胞への局在

    竹内俊二, 安藤元紀

    第9回日本耳科学会  1999 

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  • Elongated pericyte-like cells connect discrete capillaries in the stria vascularis.

    Motonori Ando, Shunji Takeuchi

    1999 

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  • A voltage-activated outward K+ current in the intermediate cell of the stria vascularis.

    Shunji Takeuchi, Motonori Ando

    1999 

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  • 中間細胞の内向き整流性K+コンダクタンスと外向き遅延整流性K+コンダクタンス

    竹内俊二, 安藤元紀

    1998 

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  • Dye-coupling of intermediate cells with endothelial cells, pericytes and basal cells in the stria vascularis of gerbils.

    Motonori Ando, Shunji Takeuchi

    1998 

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  • An inwardly rectifying K+ conductance in intermediate cells and a nonselective cation conductance in marginal cells.

    Shunji Takeuchi, Motonori Ando

    1998 

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  • 血管条における血管周皮細胞の3次元分布:蛍光免疫染色した基底膜を指標とした解析

    安藤元紀, 竹内俊二

    第8回日本耳科学会  1998 

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  • 蝸牛管側壁の毛細血管網の発達過程:蛍光ゼラチン-共焦点レーザー顕微鏡による3次元観察

    安藤元紀, 竹内俊二

    第7回日本耳科学会  1997 

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  • Dielectric dispersion of protein bound water.

    Rika Matsuoka, Makio Watanabe, Motonori Ando, Akihiko Irimajiri

    1997 

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  • Dielectric monitoring of the severity of pulmonary edema induced in the rat lung.

    Toshiyuki Yamashiro, Motonori Ando, Shohei Ogoshi, Akihiko Irimajiri

    1997 

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  • 辺縁細胞のグルコース単輸送体(GLUT):ミトコンドリア内NADHの自家蛍光を利用した生理活性の証明

    竹内俊二, 安藤元紀

    第7回日本耳科学会  1997 

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  • Dielectric dispersion analysis of erythrocyte rouleau formation.

    Motonori Ando, Makio Watanabe, Akihiko Irimajiri

    1996 

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  • Usefulness of dielectric spectroscopy in quantitative evaluation of rat cold cataract.

    Kyozo Seike, Makio Watanabe, Motonori Ando, Akihiko Irimajiri

    1996 

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  • 赤血球連銭形成時の誘電挙動

    安藤元紀, 渡辺牧夫, Laogun AA, 入交昭彦

    第47回日本生理学会中四国地方会  1995 

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  • Dielectric dispersion analysis of isolated bovine chromaffin granules and ghost.

    Motonori Ando, Akihiko Irimajiri

    1994 

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  • Dielectric dispersion analysis of the rat thyroid gland.

    Motonori Ando, Kenichi Kozakura, Haruo Saito, Akihiko Irimajiri

    1993 

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  • Dielectric spectroscopic monitoring of the formation of lung edema by use of a surface electrode.

    Toshiyuki Yamashiro, Motonori Ando, Akihiko Irimajiri

    1993 

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  • Dielectric behavior of rat submandibular gland; simulation with a vesicle-inclusion cell model.

    Kunihiko Chinzei, Motonori Ando, Haruo Saito, Akihiko Irimajiri

    1993 

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  • Dielectric analysis of the rat thyroid gland by modeling the follicle as a key structure.

    Kenichi Kozakura, Motonori Ando, Akihiko Irimajiri

    1993 

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  • 家兎外傷性前極白内障にともなう水晶体誘電挙動の変化

    渡辺牧夫, 目代康子, 上野脩幸, 安藤元紀

    第97回日本眼科学会総会  1993 

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  • ユーグレナ運動に伴う細胞インピーダンスの変化

    洲崎敏伸, 村田和義, 安藤元紀

    第25回日本原生動物学会大会  1992 

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  • Healing process of injured corneas as monitored by non-invasive dielectric spectroscopy.

    Yasuko Mokudai, Makio Watanabe, Hisayuki Ueno, Motonori Ando, Akihiko Irimajiri

    1992 

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  • Dielectric measurements of the rabbit lens in vivo using a surface probe.

    Makio Watanabe, Yasuko Mokudai, Hisayuki Ueno, Motonori Ando, Akihiko Irimajiri

    1992 

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  • Dielectric dispersion analysis of isolated chromaffin granules at low ionic strength.

    Motonori Ando, Makio Watanabe, Akihiko Irimajiri

    1992 

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  • Dielectric behavior of rat submandibular glands and its simulation with a spherical-shell model.

    Kunihiko Chinzei, Motonori Ando, Haruo Saito, Akihiko Irimajiri

    1992 

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  • 家兎水晶体の誘電挙動-眼球表面からのアプローチ

    渡辺牧夫, 目代康子, 上野脩幸, 安藤元紀, 入交昭彦

    第96回日本眼科学会総会  1992 

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  • 家兎角膜の誘電挙動-角膜上皮の創傷治癒過程

    目代康子, 渡辺牧夫, 上野脩幸, 安藤元紀, 入交昭彦.

    第96回日本眼科学会総会  1992 

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  • 誘電分散法による肺水腫評価法の基礎的検討

    山城敏行, 安藤元紀, 田宮達男, 入交昭彦

    第44回日本胸部外科学会学術集会  1991 

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  • Obserbation of axonemal microtubules in a heliozoan Echinosphaerium by polarization microscopy

    洲崎敏伸, 安藤元紀, 重中義信

    日本動物学会中国四国地方会  1991 

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  • Dielectric behavior of excised rat lung lobes in the 102-108 Hz range as correlated with pulmonary edema.

    Toshiyuki Yamashiro, Motonori Ando, Akihiko Irimajiri

    1991 

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  • 白色家兎角膜10 kHz-100 MHz領域の誘電測定

    渡辺牧夫, 小島義治, 上野脩幸, 目代康子, 安藤元紀, 入交昭彦

    第95回日本眼科学会総会  1991 

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  • 肺水腫形成時における誘電挙動の変化

    安藤元紀, 山城敏行, 入交昭彦

    第43回日本生理学会中四国地方会  1991 

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  • Dielectric dispersion analysis of isolated chromaffin granules.

    Motonori Ando, Akihiko Irimajiri

    1991 

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  • 水晶体in vivo誘電測定への取り組み

    渡辺牧夫, 小島義治, 上野脩幸, 目代康子, 安藤元紀, 入交昭彦

    第30回日本白内障学会  1991 

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  • 単離された分泌顆粒の形態計測-副腎クロマフィン顆粒の誘電解析に関連して

    安藤元紀, 渡辺牧夫, 洲崎敏伸, 入交昭彦

    第42回日本生理学会中四国地方会  1990 

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  • 太陽虫軸足の収縮運動と細胞骨格の動的変化

    重中義信, 安藤元紀

    第45回日本電子顕微鏡学会・学術講演会  1989 

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  • Quantitative polarization microscopical analysis of axonemal microtubules in a heliozoan Echinosphaerium during axopodial contraction.

    Toshinobu Suzaki, Motonori Ando, Yoshinobu Shigenaka

    1989 

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  • Mechanism of rapid axopodial contraction in Echinospaerium.

    Motonori Ando, Yoshinobu Shigenaka

    1989 

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  • The dynamic change of cytoskeleton in the helioozoan axopodium during rapid axopodial contraction.

    1989 

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  • Mechanism of axopodial contraction in heliozoa.

    Yoshinobu Shigenaka, Motonori Ando

    1988 

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  • 太陽虫軸足の収縮反応

    安藤元紀, 重中義信

    第22回日本原生動物学会大会  1988 

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Awards

  • ひらめき☆ときめきサイエンス推進賞

    2016  

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  • 日本バイオイメージング学会 ベストイメージ・カールツァイス賞

    2009  

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  • 日本循環制御医学会 会長賞

    2004  

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  • 日本循環制御医学会 ベストプレゼンテーション賞

    2003  

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Research Projects

  • 水産生物の環境DNA調査等によるブルーカーボン生態系/貯蓄モニタリングの事業化への貢献

    2025.04 - 2026.03

    文部科学省  特別電源所在県科学技術振興事業  環境・バイオ分野

    濱田麻友子, 坂本竜哉, 中田和義, 安藤元紀

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  • 水産生物の環境 DNA 調査等の“深化”によるブルーカーボン生態系の保全 産業への貢献 - 事業化へ

    2024.04 - 2025.03

    文部科学省  特別電源所在県科学技術振興事業  環境・バイオ分野

    坂本竜哉, 濱田麻友子, 中田和義, 安藤元紀

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  • 水産生物の環境DNAモニタリングの継続によるブルーカーボン生態系の保全/産業への貢献

    2023.04 - 2024.03

    文部科学省  特別電源所在県科学技術振興事業  環境・バイオ分野

    坂本竜哉, 濱田麻友子, 中田和義, 安藤元紀

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  • 環境DNAによる水産生物モニタリングの構築・産業化と次世代水産業/保全への技術・資源データ提供

    2022.04 - 2023.03

    文部科学省  特別電源所在県科学技術振興事業  環境・バイオ分野

    坂本竜哉, 濱田麻友子, 中田和義, 安藤元紀

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  • 五感ってなんだ~からだの感じるしくみを探る!

    2021.04 - 2023.03

    日本学術振興会  研究成果公開発表(B)

    安藤元紀

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  • 無腸動物の刺激受容応答機構の解明とその利用

    2021.04 - 2022.03

    水産無脊椎動物研究所  個別研究助成

    坂上登亮, 安藤元紀

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  • A novel bioassay system for antimitotic agents using unicellular organisms

    Grant number:25560416  2013.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    ANDO Motonori, ISHIKAWA Teruhiko

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    Grant amount:\3770000 ( Direct expense: \2900000 、 Indirect expense:\870000 )

    The present study provides a novel bioassay system for antimitotic agents using unicellular organisms. Heliozoon cells have many stiff and radiating axopodia, each containing a bundle of axonemal microtubules as a cytoskeleton. A flow-through type chamber was developed in this study. Heliozoon cells were more adherent to the glass coverslip then the plastic one, indicating that the substrate material influences sensitivity of this bioassay system. The axonemal microtubules were more sensitive to epothilones, a new class of microtubule-stabilizing agents with a paclitaxel-like mechanism of action. In addition, the axonemal microtubules labeled with the fluorescent paclitaxel were detected in viable heliozoon cells. These results indicate that heliozoon cells can be used as an effective in-vivo tool to screen novel microtubule-affecting agents with anti-tumor activity.

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  • ミクロの選別作業~からだの中の「もの」の動きを調節するしくみに迫ってみよう!

    2011.04 - 2019.03

    日本学術振興会  研究成果公開発表(B)

    安藤元紀

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  • Biological basis for the effectiveness of water quality monitoring system using protozoans

    Grant number:23510062  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    SUZAKI Toshinobu, YOSHIMURA Chisato, ANDO Motonori

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    Grant amount:\5460000 ( Direct expense: \4200000 、 Indirect expense:\1260000 )

    Public drinking water is vulnerable to contamination from many potential threats including pollution by agricultural, industrial and also naturally occurring chemicals. Therefore, development of effective and prompt methods for detecting harmful chemicals in drinking water is a critical issue for sustaining safety and security of the society. In spite that a variety of different procedure have been proposed for this purpose, none of them is still far from satisfactory. In this research, we have developed a dielectric monitoring system for drinking water using Euglena gracilis as a monitoring organism.

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  • Glucose transport pathways in the cochlear stria vascularis

    Grant number:22570064  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    ANDO Motonori

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    Grant amount:\5070000 ( Direct expense: \3900000 、 Indirect expense:\1170000 )

    The present study provides the first evidence that mRNAs of eight GLUT isoforms are expressed in the stria vascularis and the spiral ligament. These GLUT isoforms are suspected to involve in the high metabolic activity of the stria vascularis, and to play specific roles in maintenance of cochlear glucose homeostasis. Among these isoforms, GLUT1, -4 and -10 proteins were expressed in the strial basal cells. These findingsvalidate the transcellular glucose pathway from perilymph to intrastrial space via the basal cell layer. We also demonstrated that GLUT13 (HMIT) was expressed in the strial marginal cells, and speculate that HMIT in the marginal cells may be involved in PI synthesis, osmoregulation, and pH homeostasis of cochlear fluids.

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  • 誘電解析法を用いた新しいバイオアッセイシステムの開発

    2009.04 - 2010.03

    科学技術振興機構  シーズ発掘試験A(発掘型)

    安藤元紀

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  • Taxonomic investigation on the protozoan fauna of Japan, with special reference to the diversity crisis due to global climate change.

    Grant number:21310024  2009 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    SHIGENAKA Yoshinobu, SUZAKI Toshinobu, HAGA Nobuyuki, TSUKII Yuji, HORIGUCHI Tateo, ANDO Motonori, OTA Takashi, TANIGUCHI Akira, FUKUDA Yasuhiro, SUGIURA Mayumi, ISHIDA Hideki, SHIMANO Satoshi, HARUMOTO Terue, KITADE Osamu, KUSUOKA Yasushi, TAKAHASHI Tadao, IMAI Soichi, FUJISHIMA Masahiro, HORI Manabu, MATSUOKA Tatsuomi, ISHIDA Masaki, TAKAHASHI Mihoko, SUGIURA Mayumi, INAI Yoko, SUETOMO Yasutaka, AOKI Yoshiyuki

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    Grant amount:\18070000 ( Direct expense: \13900000 、 Indirect expense:\4170000 )

    Up to the present time, protozoan fauna in Japan has not been fully documented. Therefore, we, more than 20 protozoan specialists, have conducted a systematic survey on the Japanese protozoans by
    1) collecting original bibliographic records
    2) listing up species names and collection points, and
    3) extracting reliable list of literature, in order to construct a comprehensive inventory database of Protozoan fauna of Japan.
    We have also conducted a nation -wide field survey of free-living and parasitic protozoans at more than 100 locations in Japan, including Abashiri area in Hokkaido and Minami Daito Island in Okinawa prefecture. The result of the survey has been presented on line at http://sites.google.com/site/protistsurvey/.

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  • An aquatic bio-monitoring system using dielectric analysis

    Grant number:19510030  2007 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    SUZAKI Toshinobu, YOSHIMURA Chisato, ANDO Motonori

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    This research was aimed to develop a new approach for monitoring aquatic toxicants by using protozoan cells. A measuring device and a computer program were newly developed to monitor various morphological and electrical parameters of free-living protozoan cells in suspension. In order to evaluate feasibility of this system, Euglena gracilis was used as a test organism. As a result, the dielectric method presented here appeared to be applicable for monitoring toxicity on the cell shape and electrical properties of the plasma membrane and cell organelles.

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  • Glucose transport mechanism in the cochlea stria vascularis

    Grant number:19570058  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    ANDO Motonori

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    内耳血管条機能の根幹を支える糖輸送機構を明らかにする目的で, それに関わる糖輸送分子の組織特異的な遺伝子発現およびタンパク質発現を明らかにした。その結果, 糖輸送体分子の一つであるGLUT-1型が血管条構成細胞の中で基底細胞と血管構成細胞に発現していることや, 新たに複数のGLUT型の輸送体の遺伝子発現を確認した。血管条では複数の促進拡散型糖輸送体が連携してその高いエネルギー代謝活性をを支えているものと考えられた。

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  • Research on a novel mechanism for anti-arrhythmogenic effects of electrical vagal nerve stimulation during acute myocardial ischemia

    Grant number:17590187  2005 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    ANDO Motonori, KATARE Rajesh G.

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    Grant amount:\3500000 ( Direct expense: \3500000 )

    Myocardial ischemia (MI) leads to derangements in cellular electrical stability and the generation of lethal arrhythmias. Vagal nerve stimulation has been postulated to contribute to the antifibrillatory effect. In this study, we demonstrate a novel mechanism for the antiarrhythmogenic properties of electrical vagal nerve stimulation during acute MI. Under anesthesia, Wistar rats underwent 30 minutes of left coronary artery (LCA) ligation with vagal stimulation (MI-VS group) and with sham stimulation (MI-SS group). Eight of the 12 rats in the MI-SS group had ventricular tachyarrhythmia (VT) during 30-minute LCA ligation; on the other hand, VT occurred in only 1 of the 11 rats in the MI-VS group (67% versus 9%, respectively). Atropine administration abolished the antiarrhythmogenic effect of vagal stimulation. Immunoblotting revealed that the MI-SS group showed a marked reduction in the amount of phosphorylated connexin43 (Cx43), whereas the MI-VS group showed only a slight reduction compared with the sham operation and sham stimulation group (37+/-20% versus 79+/-18%). Immunohistochemistry confirmed that the MI-induced loss of Cx43 from intercellular junctions was prevented by vagal stimulation. In addition, studies with rat primary-cultured cardiomyocytes demonstrated that acetylcholine (ACh) effectively prevented the hypoxia-induced loss of phosphorylated Cx43 and ameliorated the loss of cell-to-cell communication as determined by Lucifer Yellow dye transfer assay, which supports the in vivo results. Furthermore, in-vitro studies also revealed that ACh induced Akt phosphorylation, which was inhibited by wortmannin. These results suggest that ACh protects cardiomyocytes through the PI3K/Akt pathway. In conclusion, vagal nerve stimulation exerts both anti-arrhythmogenic and anti-apoptotic effects during acute MI and thus plays a critical role in improving ischemia-induced electrical instability and in activating cell-survival signals.

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  • Clinical application of bionic baroreflex system

    Grant number:17300155  2005 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    SATO Takayuki, YAMASAKI Fumiyasu, USHIDA Takahiro, KAKINUMA Yoshihiko, KATARE RAJESH, ARIKAWA Mikihiko

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    Grant amount:\8300000 ( Direct expense: \8300000 )

    Background: We proposed a novel therapeutic strategy against central baroreflex failure: implementation of an artificial baroreflex system to automatically regulate sympathetic vasomotor tone, i.e., a bionic baroreflex system (BBS), and tested its efficacy in a model of sudden hypotension during surgery.
    Methods and Results : The BBS consisted of a computer-controlled negative feedback circuit that sensed arterial pressure (AP) and automatically computed the frequency (STM) of a pulse train required to stimulate sympathetic nerves via an epidural catheter placed at the level of the lower thoracic spinal cord. An operation rule was subsequently designed for the BBS using a feedback correction with proportional and integral gain factors. The transfer function from STM to AP was identified by a white-noise system-identification method in 12 sevoflurane-anesthetized patients undergoing orthopedic surgery involving the cervical vertebrae, and the feedback correction factors were determined with a numerical simulation to enable the BBS to quickly and stably attenuate an external disturbance on AP. The performance of the designed BBS was then examined in a model of orthostatic hypotension during knee joint surgery (n=21). Without the implementation of the BBS, a sudden deflation of a thigh tourniquet resulted in a 17±3 mmHg decrease in AP within 10 sec and a 25±2 mmHg decrease in AP within 50 sec. By contrast, during real-time execution of the BBS, the decrease in AP was 9±2 mmHg at 10 sec and 1±2 mmHg at 50 sec after the deflation.
    Conclusions : These results suggest the feasibility of a BBS approach for central baroreflex failure.

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  • Clinical Application of Bionic Baroreflex System

    Grant number:15300165  2003 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    SATO Takayuki, KAKINUMA Yoshihiko, YAMASAKI Fumiyasu, ANDO Motonori, KATARE Rajesh Gopalrao

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    Grant amount:\6200000 ( Direct expense: \6200000 )

    Background : We developed a bionic technology for the treatment of central baroreflex failure, and tested its efficacy in restoration of arterial pressure(AP) against a clinical model of orthostatic hypotension during surgery.
    Methods and Results : The bionic baroreflex system(BBS) was a negative feedback system controlled by a computer, which sensed AP and automatically computed the frequency (STM) of a pulse train to stimulate sympathetic nerves through an epidural catheter placed at the level of lower thoracic spinal cord. According to a classical feedback control theory, i.e., feedback correction with proportional and integral gain factors, we designed an operational rule for the BBS. We first identified the transfer function from STM to AP by a white-noise system-identification method in 12 sevoflurane-anesthetized patients who underwent an orthopedic surgery of cervical vertebrae, and then determined the feedback correction factors with a numerical simulation to enable the BBS to quickly and stably attenuate an external disturbance on AP. The performance of the designed BBS was examined in a clinical model of orthostatic hypotension (n=21). Without the implementation of the BBS, a sudden deflation of the thigh tourniquet dropped AP by 17±3 mmHg in 10 sec and by 25±2 mmHg in 50 sec. During real-time execution of the BBS, on the other hand, the fall in AP was 9±2 mmHg at 10 sec and 1±2 mmHg at 50 sec, and 0±1 mmHg at 100 sec after the deflation.
    Conclusions : The bionic technology for the artificial baroreflex system would be applicable to humans.

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  • Measurement of impedance in vivo in eye lids-development of examination of malignant lid tumors

    Grant number:14571675  2002 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    FUKUSHIMA Atsuki

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    Grant amount:\3000000 ( Direct expense: \3000000 )

    Dr.Makio Watanabe (head investigator,2002,2003) has investigated measurement of impedance in many types of cells and tissues, and has demonstrated that measurement of impedance in vitro in ocular cells and tissues was possible. Next, he succeeded to measure impedance in vivo of cornea of experimental animals. However, it has not been examined to measure corneal impedance in vivo in humans. Therefore, in this study, we aimed to measure impedance in vivo of ocular tissues in humans. First, after obtaining approval of Kochi Medical School Ethical Committee, we tried to measure corneal impedance in vivo in humans, including healthy volunteers and patients with bullous keratopathy. By using the surface electrode and the impedance analyzer 4194A (Hewlett-Packard) under the control of personal computer, impedance of cornea was measured from 10 KHz to 100 MHz. Data demonstrated that the ratio of the dispersions between low frequency and high frequency differed between healthy volunteers and patients with bullous keratopathy. The ratio was around 1.5 in healthy volunteers, while about 0.5 in patients with bullous keratopathy. Furthermore, we found the trend that a correlation existed between the ratio and the number of corneal endothelial cells. Next, we tried to measure impedance of patients with lid tumor. However, although repeated improvements of the surface electrode, we were not able to measure impedance in vivo of patients with lid tumor.

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  • 微小循環障害による内耳血管条の虚血性細胞変性メカニズムの解明

    Grant number:14770003  2002 - 2003

    日本学術振興会  科学研究費助成事業  若手研究(B)

    安藤 元紀

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    Authorship:Principal investigator 

    Grant amount:\3300000 ( Direct expense: \3300000 )

    申請者は、循環障害なしに内耳組織に適用できる新しい固定手技を開発した(14年度)。細胞質およびミトコンドリアの電子密度・体積の変化を形態計測法により検証したところ、新たに開発した固定法により、辺縁細胞の暗細胞化が抑制された。反対に虚血処理を積極的に与えた標本では、従来どおり暗細胞化がおこり、さらにミトコンドリアの膨化も誘発されていることがわかった。以上の実験結果は、極めて短時間(3分以内)の虚血処理が内耳血管条辺縁細胞に劇的な細胞内変化を引き起こすことを示している。特に、辺縁細胞の体積については虚血処理により、明らかに収縮傾向が認められ,辺縁・中間・基底細胞からなる血管条における細胞体積調節に関わるイオン輸送機構を明らかにしなければならない。
    本年度では、暗細胞化に関わる細胞レベルのイオン輸送機構のメカニズムに焦点をしぼり、細胞体積変化に着目した解析を行った。その結果、血管条内での細胞体積変化に寄与する重要な膜輸送体として、辺縁細胞の基底側膜側に発現しているNa^+-K^+-ATPaseや共輸送体のNa^+-K^+-2Cl^- cotransporterおよびCl^-チャネルが、辺縁細胞の体積変化だけではなくintra-strial sapceの体積変化にも関与していることが判明した。さらに内リンパ液の体積調節異常に起因して発症するメニエル病に関連して、水分子の輸送に関係する水チャネルのアクアポリンの血管条内での発現を検討したところ、中間細胞の細胞膜にアクアポリンファミリーの1つであるAQP1の発現をRT-PCRと免疫組織化学により確認した。以上の結果から、血管条においてはいくつかの膜輸送分子が連携してその体積調節にかかわっており、その調節系の一部のバランスが崩れることにより、様々な病態が顕在化してくるものと考えられた。今後は本研究で明らかとなった個々の機能分子が血管条全体としてどのように調節されて特定のイオンや水分子の輸送に関わっているのかを明らかにする必要がある。

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  • Cell physiological and molecular biological mechanisms of the function of the stria vascularis in the cochlea

    Grant number:12671671  2000 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    TAKEUCHI Shunji, KAKIGI Akinobu, ANDO Motonori

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    Authorship:Coinvestigator(s) 

    Grant amount:\3800000 ( Direct expense: \3800000 )

    Intermediate cells are melanocytes in the stria vascularis. To examine the contribution of the membrane potential of intermediate cells (E_m) to the endocochlear potential (EP), a comparison was made between the effects of K^+ channel blockers on the E_m and those on the EP. The E_m changed by 55.1 mV per ten-fold changes in extracellular K^+ concentration. Ba^<2+>, Cs^+ and quinine depressed the E_m in a dose-dependent manner, whereas tetraethylammonium at 30 mM and 4-aminopyridine at 10 mM had no effect. To examine the effect of the K^+ channel blockers on the EP, the EP of guinea pigs was maintained by vascular perfusion, and K^+ channel blockers were administered to the artificial blood. Ba^<2+>, Cs^+ and quinine depressed the EP in a dose-dependent manner, whereas tetraethylammonium at 30 mM and 4-aminopyridine at 10 mM did not change the EP. The changes in the EP were similar to those seen in the E_m On the basis of these results, we propose that : the EP is critically dependent on the voltage jump across the plasma membrane of intermediate cells.
    The intercellular space in the stria vascularis (intrastrial space) is a closed space and isolated from both the endolymph and the perilymph in normal tissue. Loop diuretics such as bumetanide and furosemide cause an acute enlargement of the intrastrial space in association with a decline in the endocochlear potential. It is known that bumetanide inhibits the Na^+-K^+-2Cl^- cotransporter, which is expressed abundantly in the basolateral membrane of marginal cells. We studied ionic mechanisms underlying the bumetanide-induced enlargement of the intrastrial space using perilymphatic perfusion in guinea pigs. Perilymphatic perfusion with artificial perilymph containing 100 μM bumetanide caused marked enlargement of the intrastrial space, as reported previously. Removal of K^+ from the perilymph did not affect the bumetanide-induced enlargement, whereas removal of Na^+ from the perilymph inhibited it almost completely. Perilymph containing 1 mM amiloride also inhibited the enlargement of the intrastrial space almost completely. These results indicate that perilymphatic Na^+, but not K^+, and amiloride-sensitive pathways are essential to the bumetanide-induced enlargement of the intrastrial space. Two possible pathways could yield these results. Na^+ in the perilymph could enter the endolymph via Reissner's membrane or the basilar membrane ; Na^+ in the endolymph would then be taken up by marginal cells via the apical membrane and secreted into the intrastrial space by Na^+-K^+-ATPase in the basolateral membrane of them. Another, less likely, possibility is that Na^+ in the perilymph is transported into basal cells or fibrocytes in the spiral ligament, then into intermediate cells via gap junctions, and finally secreted into the intrastrial space via Na^+-K^+-ATPase of intermediate cells.

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  • 内耳血管条上皮細胞(辺縁細胞)に発現しているユニークな体積依存性Cl-チャネル

    Grant number:12770020  2000 - 2001

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    安藤 元紀

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    Authorship:Principal investigator 

    Grant amount:\2400000 ( Direct expense: \2400000 )

    申請者により,血管条辺縁細胞の基底側膜には細胞体積と関連するCl^-チャネルが豊富に発現しており,このCl^-チャネルが内耳機能の発現に不可欠であることが明らかになっている.イオン選択性からこのチャネルがClC-superfamilyの一員であると示唆されたが,細胞体積との関連が報告されているClC-2とClC-3のどちらの電気生理学的特徴も,辺縁細胞のCl^-チャネルのものとは一致しない.そこで本研究費(初年度)により、辺縁細胞に局在しているCl^-チャネルm-RNAの同定(single-cell RT-PCR法による)を行なったところ,血管条組織レベルではClC-superfamilyのうちClC-2,-3,-5,-Klのm-RNAの発現を確認し,ClC-Klについてはsingle-cell RT-PCRを行い、辺縁細胞の単一細胞レベルでの発現を証明した。
    そこで,m-RNAレベルで血管条に発現が確認されたClC-2,-3,-5,-Klついて、免疫組織化学的手法によりその局在を明らかにすることを試みた。ClC-Klについては,特異的な抗体が入手困難であったので、チャネルタンパクに特異的なアミノ酸配列の合成オリゴペプチドに対するウサギポリクローナル抗体を作製して検討した.その結果,血管条組織レベルでClC-2,-3,K1の陽性反応を得た.ClC-5については現在検討中である.ClC-2,-3については,辺縁細胞においてClC-Klとの共発現の可能性があるため,単離血管条細胞を用いて再検討を始めている.
    以上の結果より,血管条辺縁細胞にClC-superfamilyの一部が発現していることが判明した.今後,遺伝性難聴を含めたの内耳病態とCl^-チャネルとの関わりを解明していく必要がある.

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  • 内耳血管条中間細胞(色素細胞)の内リンパ直流電位産生における役割

    Grant number:10770889  1998 - 1999

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    安藤 元紀

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    Grant amount:\1900000 ( Direct expense: \1900000 )

    1,内向き整流性K^+チャネル(K_<ir>4.1)の血管条中間細胞への局在
    ラットK_<ir>4.1のC未端側14アミノ酸残基の合成ペプチドに対するウサギポリクローナル抗体を作成し、免疫組織化学的手法により,ラットおよびスナネズミの内耳血管条内でのK_<ir>4.1の局在を明らかにした.単離細胞の免疫染色および血管条組織の免疫電顕法から,K_<ir>4.1の免疫活性は中間細胞の細胞膜のみに認められた.先に,パッチクランプ法で見出された中間細胞の内向き整流性K^+チャネル(Neurosci.Lett.247:175-178,1998)は,K_<ir>4.1である可能性が高い、(Cell& Tissue Res.298:179-183,1999).
    2,血管条中間細胞に新たに見出された電位依存性K^+チャネル
    中間細胞に見出された内向き整流性K^+チャネル(Neurosci.Lett.247:175-178,1998)に加えて,新たに電位依存性K^+チャネルの存在をパッチクランプ法で明らかにした.このチャネルは,中間細胞の膜電位の安定化に寄与し,内向き整流性K^+チャネルとは別のK^+の分泌ルートになりうると考えられ,内向き整流性K^+チャネルとともに,内リンパ直流電位の生成と維待に主要な役割を果たしている可能性が高い(Am.J.Physiol.277:C91-C99.1999).

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  • Quantification of rouleaux formation by red cells in whole blood

    Grant number:09672359  1997 - 1998

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    IRIMAJIRI Akihiko, IMAMURA Jun, ANDO Tomonari, RAICU Valerica, ICHINOWATARI Takako

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    Grant amount:\3000000 ( Direct expense: \3000000 )

    To develop a quick method of obtaining a practical index replaceable for the conventional erythrocyte sedimentation rate (ESR), we examined the possibility of applying the dielectric spectroscopy to the measurement of rouleaux formation in whole blood. The main findings follow.
    (1) Rouleaux formation is a key determinant of the ESR.
    (2) The degree of rouleaux formation in whole blood is readily detectable through measurements of relative permittivity (ε) for the blood at low frequencies (10-100 kHz).
    (3) We fabricated a rotary cell (I.e. a sample housing with a pair of electrodes as the sensor) with which to measure c while changing the shear stress to be exerted on the blood sample.
    (4) Upon varying the shear rate stepwise, the low-frequency level of ε traced stepwise decreases with increasing shear rate and showed a mirror image with decreasing shear rate.
    (5) These stepwise changes in whole blood s could be correlated with ESR.
    (6) Another approach was also pursued which employed a pair of open-ended coaxial probes set at both ends of a blood-filled, vertical, Perspex column. This method enabled a simultaneous determination of ESR and low-frequency ε. Preliminary experiments indicated that these two separate indices correlated well with each other.

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  • 甲状腺の単離濾胞を用いた上皮細胞構築と膜イオン・チャネルの動態解析

    Grant number:06670058  1994

    日本学術振興会  科学研究費助成事業  一般研究(C)

    入交 昭彦, 市ノ渡 孝子, 安藤 元紀, 竹内 俊二

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    (1)ブタ甲状腺の新鮮標品をコラゲナーゼ処理することにより,直径100〜200μmのほゞ球形の単離濾胞が得られた.
    (2)位相差あるいは微分干渉光学系により,濾胞上皮細胞の底面像および縦断面像が観察できた.生理的培養液中では,この上皮細胞は正常の形態を比較的長時間にわたり維持することがわかった.
    (3)フロー・セルを作製し,種々の外液中における濾胞および細胞の形態変化を追跡した.低張液(蒸溜水)で潅流すると,濾胞の外内径ともに,時間とともにほゞ直線的に増大した.潅流開始10分後までは,上皮細胞の膨化を伴なうものゝ,顕著な細胞破裂像は認めなかった.これは,濾胞内腔(コロイド)より供給されるイオンなどOSmolytesの滲透圧的拮抗作用によるものと推定される.15EA04:(4)このような単離濾胞を対象とする非侵襲的誘電泳動測定装置を開発したので,これによる細胞・コロイドの電気的性質および膜チャネルの解析を進めている.

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  • 誘電スペクトル法による肺組織機能動態の無侵襲解析

    Grant number:05670044  1993

    日本学術振興会  科学研究費助成事業  一般研究(C)

    入交 昭彦, 山城 敏行, 市ノ渡 孝子, 竹内 俊二, 安藤 元紀

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    Grant amount:\2000000 ( Direct expense: \2000000 )

    誘電スペクトル解析法により,正常肺および異常肺(水腫肺)における生体組織中での肺胞の機能動態を,肺の組織構築を壊すことなく追跡することを試みた。
    1)摘出肺葉(正常)の誘電挙動
    【.encircled1.】100Hz-500MHzにわたる広帯域精密誘電測定をおこない,誘電スペクトル特性と含気率との相関を確定した.
    【.encircled2.】形態情報を考慮した肺組織の誘電体モデルをつくり,肺bulk impedanceの周波数依存性を濃厚分散系の誘電理論を用いて予測した.
    【.encircled3.】In situ肺の誘電測定に応用可能な「同心円型表面電極」を試作し,従来の平行板型電極によって得られた誘電特性量との異同について調べた.10kHz〜100MHz域においては,ほぼ同一の誘電挙動がみと認められ,同心円型表面電極がin situ測定に有用であることが判明した.
    2)肺水腫生成過程の追跡
    【.encircled1.】同心円型表面電極を用いて,実験的肺水腫の生成過程を誘電挙動の変化として捉えた.肺循環灌流圧および気道内圧を変化させることにより,誘電挙動に著明な差異が生じた.
    【.encircled2.】特性量〓epsilon,〓kappaおよびloss tangent値に着目すると,時間経過とともに増加傾向を示したので,肺湿重量の変化をよく反映していることが判った.これらのパラメータが肺水腫の重症度の評価に際し,有用な指標を与えるものと考えられる.
    3)In situ肺の誘電測定
    摘出肺へのアプローチからさらに進めて,in situ肺の誘電挙動について調べている.開胸露出した肺葉の表面に電極を密着させ,肺気量を周期的に変化させた場合,肺内気量の変化にあわせて誘電挙動も変化することが判明した.

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  • 副腎髄質細胞における分泌顆粒動態の誘電スペクトル解析

    Grant number:05770031  1993

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    安藤 元紀

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    Grant amount:\900000 ( Direct expense: \900000 )

    誘電スペクトル解析法により,単離副腎クロマフィン顆粒の誘電挙動を調べ,顆粒限界膜および内相の電気的パラメータの推定を試みた.
    1.形態計測
    (1)単離クロマフィン細胞の体積変化を最小限にとどめる電顕用固定液の浸透圧条件を調べたところ,細胞を懸濁している溶液よりやや高めに設定する必要のあることが判明した.
    (2)(1)で設定した固定液を用いて電顕レベルでの単離クロマフィン顆粒の形態計測をおこない,さらにステレオロジー理論に基づて顆粒直径の真の分布を推定した.
    2.誘電測定および理論解析
    (1)10kHz-500MHzにわたる精密誘電測定をおこない,クロマフィン顆粒の誘電スペクトル特性を確定した.
    (2)形態計測データを用いて,“one-shell"モデルにより理論解析したところ,実測値を十分に再現することはできなかった.これは顆粒限界膜が単純な一層の“shell"で説明できないことを意味する.
    (3)単離クロマフィン顆粒を低イオン強度溶液中に懸濁した場合,顆粒限界膜の表面電荷に起因するsurface conductivityを取り入れた“multi-shell"モデルにより,はじめて実測誘電挙動を再現できた.

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  • 肺組織機能動態の誘電スペクトル解析

    Grant number:04670050  1992

    日本学術振興会  科学研究費助成事業  一般研究(C)

    入交 昭彦, 山城 敏行, 市ノ渡 孝子, 安藤 元紀

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    Authorship:Coinvestigator(s) 

    Grant amount:\2000000 ( Direct expense: \2000000 )

    誘電スペクトル解析法により、正常肺および異常肺(水腫肺)について生組織中での肺胞の機能動態を、肺の組織構築を壊すことなく追跡することを試みた。
    1)摘出肺葉(正常)の誘電測定 (1)100Hz〜500MHz域にわたる精密誘電測定をおこない、誘電スペクトル特性と含気率との関係を確定した。
    (2)形態情報を組み入れた肺組織の誘電体モデルをつくり、肺bulk impedanceの周波数依存性を濃厚分散系の誘電理論を用いて予測した。
    (3)In situ肺の誘電測定に適用できる「同心円型表面電極」を試作し、従来の平行板型電極によって得られた誘電特性量との異同について調ベた。10kHz〜100MHz域においては、ほぼ同一の誘電挙動が認められ、同心円型表面電極がin situ測定に有用であることが判明した。
    2)肺水腫生成過程の追跡
    (1)同心円型表面電極を用いて、実験的肺水腫の生成過程を誘電挙動の変化として捉えた。肺循環灌流圧および気道内圧を変化させることにより誘電挙動に著明な差異が生じた。
    (2)特性量Δε,Δκおよびloss tangent値に着目すると、時間経過とともに増加傾向を示したので、肺湿重量の変化をよく反映することが判った。これらのパラメータが肺水腫の重症度の評価に際し、有用な指標を与えるものと考えられる。
    3)In situ肺の誘電測定
    摘出肺からさらにすすめて、in situ肺の誘電測定について調べている。開胸露出した肺葉の表面に電極を密着させ、肺気量を周期的に変えた場合、肺内気量の変化とともに測定値が変化することが判明した。

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  • 誘電分散法による肺組織機能動態の解析ー「肺胞」モデルを中心に

    Grant number:03671084  1991

    日本学術振興会  科学研究費助成事業  一般研究(C)

    入交 昭彦, 山城 敏行, 市ノ渡 孝子, 安藤 元紀

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    Authorship:Coinvestigator(s) 

    Grant amount:\2000000 ( Direct expense: \2000000 )

    当教室において開発された「細胞誘電解析法」の基礎の上に,肺胞・細気管支および毛細管の三者を考慮した肺組織の誘電体モデルを提出し,正常肺および異常肺(水腫肺・無気肺)について生組織中での肺胞の機能動態を,肺の組織構築を壊すことなく追跡することを試みた。
    1)摘出肺葉の誘電測定:まず,ラット単離肺葉のbulk impedance測定用として,平行板コンデンサ-型のセルを試作した。これは,気道系および血管系を別個に確保し,陰圧呼吸が可能,両系の容積と圧が独立に変えられるように設計した。これを用いて,(1)100Hz〜500MHz域にわたり精密誘電測定をおこない誘電スペクトル特性と含気率との関係を確定した。(2)潅流固定標本のstereologyから肺胞と血管の形態計測をおこなった。(3)これらの形態情報を組み入れた肺組織の誘電体モデルをつくり,肺bulk impedanceの周波数依存性を濃厚分散系の誘電理論を用いて予測した。
    2)無気肺,実験的水腫肺についても同様の測定をおこなった。コントロ-ル群,軽度および重度肺水腫群の間で誘電挙動に著明な差異がみられた。特性量ΔεおよびΔηに着目すると3群間に有意差を認めたので,インピ-ダンス法は肺水腫の重症度の評価に際し,有用な指標を与えるものと考えられる。
    3)摘出肺からさらにすすめて,in situ肺の誘電測定についても,「同心円型表面電極」を試作し,従来の平行板型電極によって得られた誘電特性量との異同について調べている。10kHz〜100MHz間においては,ほぼ同一の測定値が得られることが判明した。

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  • 新しい細胞運動・細胞骨格系の探索とその応用技術の開発

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    Grant type:Competitive

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  • 生体インピーダンス測定による非侵襲的組織解析法の開発

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    Grant type:Competitive

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  • 循環動態を解析する基盤技術の開発

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    Grant type:Competitive

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  • 内耳蝸牛のイオン輸送機構と糖輸送機構の解明

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