MISC - KANZAKI Hiroshi

Insight into the active site of Streptomyces cystathionine γ-lyase based on the results of studies on its substrate specificity

KANZAKI H.

Biochim. Biophys. Acta   913   45 - 50   1987

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Highly efficient production of l-cystathionine from O-succinyl-l-homoserine and l-cysteine by Streptomyces cystathionine γ-lyase

Hiroshi Kanzaki, Toru Nagasawa, Hideaki Yamada

Applied Microbiology and Biotechnology   25 ( 2 )   97 - 100   1986

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Language：English   Publisher：Springer-Verlag  

Utilizing the γ-replacement reaction of Streptomyces cystathionine γ-lyase (EC 4.4.1.1.), an efficient production method for l-cystathionine has been established. Under optimal conditions, 50 mM l-cystathionine was synthesized from 50 mM O-succinyl-l-homoserine and 50 mM l-cysteine, added in four stages to the reaction mixture, with a substrate conversion rate of 100%. This productivity (11 gl-1 of reaction mixture) is about 3.5 times higher than that with l-homoserine and l-cysteine as substrates. © 1986 Springer-Verlag.

DOI： 10.1007/BF00250516

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