Updated on 2025/09/30

写真a

 
MITANI-Ueno Namiki
 
Organization
Scheduled update Associate Professor
Position
Associate Professor
Homepage
http://www.rib.okayama-u.ac.jp/plant.stress/index-j.html
External link

Degree

  • 博士（農学） ( 2008.9   岡山大学 )

Research Interests

  • Plant nutrition

  • 植物ストレス

  • 輸送体

  • transporter

  • 植物栄養

  • Plant stress

Research Areas

  • Life Science / Plant nutrition and soil science

Education

  • Okayama University   自然科学研究科バイオサイエンス専攻  

    2006.4 - 2008.9

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Research History

  • -

    2017

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  • Okayama University   Institute of Plant Science and Resources

    2013 - 2017

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  • Okayama University   Institute of Plant Science and Resources

    2010 - 2013

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Professional Memberships

 

Papers

  • Shoot-Silicon-Signal protein to regulate root silicon uptake in rice Reviewed

    Naoki Yamaji, Namiki Mitani-Ueno, Toshiki Fujii, Tomonori Shinya, Ji Feng Shao, Shota Watanuki, Yasunori Saitoh, Jian Feng Ma

    Nature Communications   15 ( 1 )   2024.12

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1038/s41467-024-55322-7

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    Other Link： https://www.nature.com/articles/s41467-024-55322-7

  • Comprehensive analysis of silicon impact on defense and metabolic responses in rice exposed to herbivory stress Reviewed

    Dandy Ahamefula Osibe, Yuko Hojo, Tomonori Shinya, Namiki Mitani-Ueno, Ivan Galis

    Frontiers in Plant Science   15   2024.5

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    Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    Silicon (Si) uptake is generally beneficial for plants that need protection from insect herbivores. In pursue of mechanisms involved in Si-mediated defense, we comprehensively explored the impact of Si on several defensive and metabolic traits in rice exposed to simulated and real herbivory of Mythimna loreyi Duponchel larvae. Hydroponic experiments showed that Si-deprived rice supplemented with Si 72 h prior to insect infestation were similarly resistant to larvae as plants continuously grown in Si-containing media. Both Si and herbivory altered primary metabolism in rice, including the levels of several sugars, amino acids, and organic acids. While the accumulation of sugars was generally positively correlated with Si presence, multiple amino acids showed a negative correlation trend with Si supplementation. The levels of secondary metabolites, including isopentylamine, p-coumaroylputrescine and feruloylputrescine, were typically higher in the leaves of Si-supplemented plants exposed to herbivory stress compared to Si-deprived plants. In addition, simulated herbivory treatment in Si-supplemented plants induced more volatile emissions relative to Si-deprived plants, which was consistent with the increased transcripts of key genes involved in volatile biosynthesis. In ecological interactions, Si alone did not affect the oviposition choice of M. loreyi but gravid females showed a significant preference for simulated herbivory-treated/Si-deprived compared to Si-supplemented plants. Our data suggest that apart from mechanical defense, Si may affect rice metabolism in multiple ways that might enhance/modulate defense responses of rice under herbivory stress.

    DOI： 10.3389/fpls.2024.1399562

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  • Knockout of a rice K5.2 gene increases Ca accumulation in the grain. Reviewed International journal

    Peitong Wang, Naoki Yamaji, Namiki Mitani-Ueno, Jun Ge, Jian Feng Ma

    Journal of integrative plant biology   2024.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    Rice is a staple food for half world's population, but it is a poor dietary source of calcium (Ca) due to its low concentration. Therefore, it is an important issue to boost the Ca concentration in the grain for improving Ca deficiency risk, but the mechanisms underlying the Ca accumulation are poorly understood. Here, we obtained a rice (Oryza sativa) mutant with high shoot Ca accumulation. The mutant exhibited 26-53% higher Ca in the shoots than that in wild-type rice (WT) at different Ca supplies. The Ca concentration in the xylem sap was 36% higher in the mutant than in the WT. There was no difference in agronomic traits between the WT and mutant, but the mutant showed 25% higher Ca in the polished grain compared with the WT. Map-based cloning combined with complementation test revealed that the mutant phenotype was caused by an 18-bp deletion of a gene, OsK5.2 belonging to Shaker-like K+ channel family. OsK.2 was highly expressed in the mature region of the roots and its expression in the roots was not affected by Ca levels, but up-regulated by low K. Immunostaining showed that OsK.2 was mainly expressed in pericycle of the roots. Taken together, our results revealed a novel role of OsK.2 in Ca translocation in rice, which will be a good target for Ca biofortification in rice. This article is protected by copyright. All rights reserved.

    DOI： 10.1111/jipb.13587

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  • A silicon transporter gene required for healthy growth of rice on land. Reviewed International journal

    Namiki Mitani-Ueno, Naoki Yamaji, Sheng Huang, Yuma Yoshioka, Takaaki Miyaji, Jian Feng Ma

    Nature communications   14 ( 1 )   6522 - 6522   2023.10

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    Silicon (Si) is the most abundant mineral element in the earth's crust. Some plants actively accumulate Si as amorphous silica (phytoliths), which can protect plants from stresses. Here, we report a gene (SIET4) that is required for the proper accumulation and cell-specific deposition of Si in rice and show that it is essential for normal growth. SIET4 is constitutively expressed in leaves and encodes a Si transporter. SlET4 polarly localizes at the distal side of epidermal cells and cells surrounding the bulliform cells (motor cells) of the leaf blade, where Si is deposited. Knockout of SIET4 leads to the death of rice in the presence but not absence of Si. Further analysis shows that SIET4 knockout induces abnormal Si deposition in mesophyll cells and the induction of hundreds of genes related to various stress responses. These results indicate that SIET4 is required for the proper export of Si from leaf cells to the leaf surface and for the healthy growth of rice on land.

    DOI： 10.1038/s41467-023-42180-y

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  • Polar localization of a rice silicon transporter requires isoleucine at both C- and N-termini as well as positively charged residues. Reviewed International journal

    Noriyuki Konishi, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    The Plant cell   35 ( 6 )   2232 - 2250   2023.5

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    Silicon (Si) is important for stable and high yields in rice (Oryza sativa), a typical Si hyperaccumulator. The high Si accumulation is achieved by the cooperation of 2 Si transporters, LOW SILICON 1 (OsLsi1) and OsLsi2, which are polarly localized in cells of the root exodermis and endodermis. However, the mechanism underlying their polar localization is unknown. Here, we identified amino acid residues critical for the polar localization of OsLsi1. Deletion of both N- and C-terminal regions resulted in the loss of its polar localization. Furthermore, the deletion of the C-terminus inhibited its trafficking from the endoplasmic reticulum to the plasma membrane. Detailed site-directed mutagenesis analysis showed that Ile18 at the N-terminal region and Ile285 at the C-terminal region were essential for the polar localization of OsLsi1. Moreover, a cluster of positively charged residues at the C-terminal region is also required for polar localization. Phosphorylation and Lys modifications of OsLsi1 are unlikely to be involved in its polar localization. Finally, we showed that the polar localization of OsLsi1 is required for the efficient uptake of Si. Our study not only identified critical residues required for the polar localization of OsLsi1, but also provided experimental evidence for the importance of transporter polarity for efficient nutrient uptake.

    DOI： 10.1093/plcell/koad073

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  • A pericycle‐localized silicon transporter for efficient xylem loading in rice Reviewed

    Sheng Huang, Naoki Yamaji, Gen Sakurai, Namiki Mitani‐Ueno, Noriyuki Konishi, Jian Feng Ma

    New Phytologist   234 ( 1 )   197 - 208   2022.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1111/nph.17959

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    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.17959

  • Boron uptake in rice is regulated post-translationally via a clathrin-independent pathway Reviewed

    Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Ji Feng Shao, Namiki Mitani-Ueno, Jian Feng Ma

    Plant Physiology   188 ( 3 )   1649 - 1664   2022.3

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    Uptake of boron (B) in rice (Oryza sativa) is mediated by the Low silicon rice 1 (OsLsi1) channel, belonging to the NOD26-like intrinsic protein III subgroup, and the efflux transporter B transporter 1 (OsBOR1). However, it is unknown how these transporters cooperate for B uptake and how they are regulated in response to B fluctuations. Here, we examined the response of these two transporters to environmental B changes at the transcriptional and posttranslational level. OsBOR1 showed polar localization at the proximal side of both the exodermis and endodermis of mature root region, forming an efficient uptake system with OsLsi1 polarly localized at the distal side of the same cell layers. Expression of OsBOR1 and OsLsi1 was unaffected by B deficiency and excess. However, although OsLsi1 protein did not respond to high B at the protein level, OsBOR1 was degraded in response to high B within hours, which was accompanied with a significant decrease of total B uptake. The high B-induced degradation of OsBOR1 was inhibited in the presence of MG-132, a proteasome inhibitor, without disturbance of the polar localization. In contrast, neither the high B-induced degradation of OsBOR1 nor its polarity was affected by induced expression of dominant-negative mutated dynamin-related protein 1A (OsDRP1AK47A) or knockout of the mu subunit (AP2M) of adaptor protein-2 complex, suggesting that clathrin-mediated endocytosis is not involved in OsBOR1 degradation and polar localization. These results indicate that, in contrast to Arabidopsis thaliana, rice has a distinct regulatory mechanism for B uptake through clathrin-independent degradation of OsBOR1 in response to high B.

    DOI： 10.1093/plphys/kiab575

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    Other Link： https://academic.oup.com/plphys/article-pdf/188/3/1649/42744142/kiab575.pdf

  • A crucial role for a node-localized transporter, HvSPDT, in loading phosphorus into barley grains. Reviewed International journal

    Mian Gu, Hengliang Huang, Hiroshi Hisano, Guangda Ding, Sheng Huang, Namiki Mitani-Ueno, Kengo Yokosho, Kazuhiro Sato, Naoki Yamaji, Jian Feng Ma

    New phytologist   2022.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    Grains are the major sink of phosphorus (P) in cereal crops, accounting for 60-85% of total plant P, but the mechanisms underlying P loading into the grains are poorly understood. We functionally characterized a transporter gene required for the distribution of P to the grains in barley (Hordeum vulgare), HvSPDT (SULTR-like phosphorus distribution transporter). HvSPDT encoded a plasma membrane-localized Pi/H+ cotransporter. It was mainly expressed in the nodes at both the vegetative and reproductive stages. Furthermore, its expression was induced by inorganic phosphate (Pi) deficiency. In the nodes, HvSPDT was expressed in both the xylem and phloem region of enlarged and diffuse vascular bundles. Knockout of HvSPDT decreased the distribution of P to new leaves, but increased the distribution to old leaves at the vegetative growth stage under low P supply. However, knockout of HvSPDT did not alter the redistribution of P from old to young organs. At the reproductive stage, knockout of HvSPDT significantly decreased P allocation to the grains, resulting in a considerable reduction in grain yield, especially under P-limited conditions. Our results indicate that node-based HvSPDT plays a crucial role in loading P into barley grains through preferentially distributing P from the xylem and further to the phloem.

    DOI： 10.1111/nph.18057

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  • Structural basis for high selectivity of a rice silicon channel Lsi1 Reviewed International journal

    Yasunori Saitoh, Namiki Mitani-Ueno, Keisuke Saito, Kengo Matsuki, Sheng Huang, Lingli Yang, Naoki Yamaji, Hiroshi Ishikita, Jian-Ren Shen, Jian Feng Ma, Michihiro Suga

    Nature Communications   12 ( 1 )   6236 - 6236   2021.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    <title>Abstract</title>Silicon (Si), the most abundant mineral element in the earth’s crust, is taken up by plant roots in the form of silicic acid through Low silicon rice 1 (Lsi1). Lsi1 belongs to the Nodulin 26-like intrinsic protein subfamily in aquaporin and shows high selectivity for silicic acid. To uncover the structural basis for this high selectivity, here we show the crystal structure of the rice Lsi1 at a resolution of 1.8 Å. The structure reveals transmembrane helical orientations different from other aquaporins, characterized by a unique, widely opened, and hydrophilic selectivity filter (SF) composed of five residues. Our structural, functional, and theoretical investigations provide a solid structural basis for the Si uptake mechanism in plants, which will contribute to secure and sustainable rice production by manipulating Lsi1 selectivity for different metalloids.

    DOI： 10.1038/s41467-021-26535-x

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    Other Link： https://www.nature.com/articles/s41467-021-26535-x

  • A Low Level of NaCl Stimulates Plant Growth by Improving Carbon and Sulfur Assimilation in Arabidopsis thaliana Reviewed

    Li Hongqiao, Akiko Suyama, Namiki Mitani-Ueno, Ruediger Hell, Akiko Maruyama-Nakashita

    Plants   10 ( 10 )   2138 - 2138   2021.10

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    High-salinity stress represses plant growth by inhibiting various metabolic processes. In contrast to the well-studied mechanisms mediating tolerance to high levels of salt, the effects of low levels of salts have not been well studied. In this study, we examined the growth of Arabidopsis thaliana plants under different NaCl concentrations. Interestingly, both shoot and root biomass increased in the presence of 5 mM NaCl, whereas more than 10 mM NaCl decreased plant biomass. To clarify the biological mechanism by which a low level of NaCl stimulated plant growth, we analyzed element accumulation in plants grown under different NaCl concentrations. In addition to the Na and Cl contents, C, S, Zn, and Cu contents were increased under 5 mM NaCl in shoots; this was not observed at higher NaCl concentrations. Adverse effects of high salinity, such as decreased levels of nitrate, phosphate, sulfate, and some cations, did not occur in the presence of 5 mM NaCl. An increase in C was possibly attributed to increased photosynthesis supported by Cl, Zn, and Cu, which also increased in shoots after NaCl application. Salt stress-responsive gene expression was enhanced under 20 mM NaCl but not at lower doses. Among the S metabolites analyzed, cysteine (Cys) was increased by 5 mM NaCl, suggesting that S assimilation was promoted by this dose of NaCl. These results indicate the usefulness of NaCl for plant growth stimulation.

    DOI： 10.3390/plants10102138

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  • Linking transport system of silicon with its accumulation in different plant species Invited Reviewed

    Namiki Mitani-Ueno, Jian Feng Ma

    Soil Science and Plant Nutrition   67 ( 1 )   10 - 17   2021.1

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/00380768.2020.1845972

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  • The grapevine NIP2;1 aquaporin is a silicon channel Reviewed

    Henrique Noronha, Angelica Silva, Namiki Mitani-Ueno, Carlos Conde, Farzana Sabir, Catarina Prista, Graca Soveral, Paul Isenring, Jian Feng Ma, Richard R. Belanger, Hernani Geros

    JOURNAL OF EXPERIMENTAL BOTANY   71 ( 21 )   6789 - 6798   2020.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/jxb/eraa294

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  • Node-Localized Transporters of Phosphorus Essential for Seed Development in Rice. Reviewed

    Jing Che, Naoki Yamaji, Takaaki Miyaji, Namiki Mitani-Ueno, Yuri Kato, Ren Fang Shen, Jian Feng Ma

    Plant & cell physiology   61 ( 8 )   1387 - 1398   2020.8

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    About 60-85% of total phosphorus (P) in cereal crops is finally allocated to seeds, where it is required for seed development, germination and early growth. However, little is known about the molecular mechanisms underlying P allocation to seeds. Here, we found that two members (OsPHO1;1 and OsPHO1;2) of the PHO1 gene family are involved in the distribution of P to seeds in rice. Both OsPHO1;1 and OsPHO1;2 were localized to the plasma membrane and showed influx transport activities for inorganic phosphate. At the reproductive stage, both OsPHO1;1 and OsPHO1;2 showed higher expression in node I, the uppermost node connecting to the panicle. OsPHO1;1 was mainly localized at the phloem region of diffuse vascular bundles (DVBs) of node I, while OsPHO1;2 was expressed in the xylem parenchyma cells of the enlarged vascular bundles (EVBs). In addition, they were also expressed in the ovular vascular trace, the outer layer of the inner integument (OsPHO1;1) and in the nucellar epidermis (OsPHO1;2) of caryopses. Knockout of OsPHO1;2, as well as OsPHO1;1 to a lesser extent, decreased the distribution of P to the seed, resulting in decreased seed size and delayed germination. Taken together, OsPHO1;2 expressed in node I is responsible for the unloading of P from the xylem of EVBs, while OsPHO1;1 is involved in reloading P into the phloem of DVBs for subsequent allocation of P to seeds. Furthermore, OsPHO1;1 and OsPHO1;2 expression in the caryopsis is important for delivering P from the maternal tissues to the filial tissues for seed development.

    DOI： 10.1093/pcp/pcaa074

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  • The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions(1)([OPEN]) Reviewed

    Sheng Huang, Akimasa Sasaki, Naoki Yamaji, Haruka Okada, Namiki Mitani-Ueno, Jian Feng Ma

    PLANT PHYSIOLOGY   183 ( 3 )   1224 - 1234   2020.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1104/pp.20.00125

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  • Tomato roots have a functional silicon influx transporter but not a functional silicon efflux transporter Reviewed

    Hao Sun, Yaoke Duan, Namiki Mitani-Ueno, Jing Che, Jianhua Jia, Jiaqi Liu, Jia Guo, Jian Feng Ma, Haijun Gong

    PLANT CELL AND ENVIRONMENT   43 ( 3 )   732 - 744   2020.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/pce.13679

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  • Functional evolution of nodulin 26-like intrinsic proteins: from bacterial arsenic detoxification to plant nutrient transport Reviewed

    Benjamin Pommerrenig, Till A. Diehn, Nadine Bernhardt, Manuela D. Bienert, Namiki Mitani-Ueno, Jacqueline Fuge, Annett Bieber, Christoph Spitzer, Andrea Braeutigam, Jian Feng Ma, Francois Chaumont, Gerd P. Bienert

    NEW PHYTOLOGIST   225 ( 3 )   1383 - 1396   2020.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/nph.16217

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  • Vascular Cambium-Localized AtSPDT Mediates Xylem-to-Phloem Transfer of Phosphorus for Its Preferential Distribution in Arabidopsis Reviewed

    Guangda Ding, Gui Jie Lei, Naoki Yamaji, Kengo Yokosho, Namiki Mitani-Ueno, Sheng Huang, Jian Feng Ma

    MOLECULAR PLANT   13 ( 1 )   99 - 111   2020.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.molp.2019.10.002

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  • Transport system of mineral elements in rice Reviewed

    Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    Rice Genomics, Genetics and Breeding   223 - 240   2018.3

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    Language：English   Publishing type：Part of collection (book)   Publisher：Springer Singapore  

    DOI： 10.1007/978-981-10-7461-5_13

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  • The tonoplast-localized transporter MTP8.2 contributes to manganese detoxification in the shoots and roots of oryza sativa L. Reviewed

    Yuma Takemoto, Yuta Tsunemitsu, Miho Fujii-Kashino, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma, Shin Ichiro Kato, Kozo Iwasaki, Daisei Ueno

    Plant and Cell Physiology   58 ( 9 )   1573 - 1582   2017.9

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcx082

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  • A model of silicon dynamics in rice: An analysis of the investment efficiency of Si transporters Reviewed

    Gen Sakurai, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Keisuke Ono, Jian Feng Ma

    Frontiers in Plant Science   8   E31-E34   2017.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media S.A.  

    DOI： 10.3389/fpls.2017.01187

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  • A Model of Silicon Dynamics in Rice: An Analysis of the Investment Efficiency of Si Transporters Reviewed

    Gen Sakurai, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Keisuke Ono, Jian Feng Ma

    FRONTIERS IN PLANT SCIENCE   8   2017.7

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    DOI： 10.3389/10E-2017.01187

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  • Identification of a mammalian silicon transporter Reviewed

    Sarah Ratcliffe, Ravin Jugdaohsingh, Julien Vivancos, Alan Marron, Rupesh Deshmukh, Jian Feng Ma, Namiki Mitani-Ueno, Jack Robertson, John Wills, Mark V. Boekschoten, Michael Mueller, Robert C. Mawhinney, Stephen D. Kinrade, Paul Isenring, Richard R. Belanger, Jonathan J. Powell

    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY   312 ( 5 )   C550 - C561   2017.5

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    DOI： 10.1152/ajpcell.00219.2015

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  • Polar Localization of the NIP5;1 Boric Acid Channel Is Maintained by Endocytosis and Facilitates Boron Transport in Arabidopsis Roots Reviewed

    Sheliang Wang, Akira Yoshinari, Tomoo Shimada, Ikuko Hara-Nishimura, Namiki Mitani-Ueno, Jian Feng Ma, Satoshi Naito, Junpei Takano

    PLANT CELL   29 ( 4 )   824 - 842   2017.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1105/tpc.16.00825

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  • Reducing phosphorus accumulation in rice grains with an impaired transporter in the node (vol 541, pg 92, 2017) Reviewed

    Naoki Yamaji, Yuma Takemoto, Takaaki Miyaji, Namiki Mitani-Ueno, Kaoru T. Yoshida, Jian Feng Ma

    NATURE   541 ( 7635 )   136 - 136   2017.1

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  • High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice Reviewed

    Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   57 ( 12 )   2510 - 2518   2016.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcw163

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  • An Aluminum-Inducible IREG Gene is Required for Internal Detoxification of Aluminum in Buckwheat Reviewed

    Kengo Yokosho, Naoki Yamaji, Namiki Mitani-Ueno, Ren Fang Shen, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   57 ( 6 )   1169 - 1178   2016.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcw065

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  • A node-localized transporter OsZIP3 is responsible for the preferential distribution of Zn to developing tissues in rice Reviewed

    Akimasa Sasaki, Naoki Yamaji, Namiki Mitani-Ueno, Miho Kashino, Jian Feng Ma

    PLANT JOURNAL   84 ( 2 )   374 - 384   2015.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/tpj.13005

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  • Orchestration of three transporters and distinct vascular structures in node for intervascular transfer of silicon in rice Reviewed

    Naoki Yamaji, Gen Sakurai, Namiki Mitani-Ueno, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   112 ( 36 )   11401 - 11406   2015.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1073/pnas.1508987112

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  • In Silico Simulation Modeling Reveals the Importance of the Casparian Strip for Efficient Silicon Uptake in Rice Roots Reviewed

    Gen Sakurai, Akiko Satake, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Francois Gabriel Feugier, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   56 ( 4 )   631 - 639   2015.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcv017

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  • A small RNA mediated regulation of a stress-activated retrotransposon and the tissue specific transposition during the reproductive period in Arabidopsis Reviewed

    Wataru Matsunaga, Naohiko Ohama, Noriaki Tanabe, Yukari Masuta, Seiji Masuda, Namiki Mitani, Kazuko Yamaguchi-Shinozaki, Jian F. Ma, Atsushi Kato, Hidetaka Ito

    FRONTIERS IN PLANT SCIENCE   6   48   2015.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3389/fpls.2015.00048

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  • Transcriptional activity and silencing of heat activated retrotransposon in Arabidopsis Reviewed

    Wataru Matsunaga, Yukari Masuta, Namiki Mitani, Ma Jian Feng, Atsushi Kato, Hidetaka Ito

    GENES & GENETIC SYSTEMS   89 ( 6 )   316 - 316   2014.12

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  • Physiological and molecular characterization of Si uptake in wild rice species Reviewed

    Namiki Mitani-Ueno, Hisao Ogai, Naoki Yamaji, Jian Feng Ma

    PHYSIOLOGIA PLANTARUM   151 ( 3 )   200 - 207   2014.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/ppl.12125

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  • Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation Reviewed

    Matthew J. Milner, Namiki Mitani-Ueno, Naoki Yamaji, Kengo Yokosho, Eric Craft, Zhangjun Fei, Stephen Ebbs, M. Clemencia Zambrano, Jian Feng Ma, Leon V. Kochian

    PLANT JOURNAL   78 ( 3 )   398 - 410   2014.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/tpj.12480

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  • Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2 Reviewed

    Naoki Yamaji, Jixing Xia, Namiki Mitani-Ueno, Kengo Yokosho, Jian Feng Ma

    PLANT PHYSIOLOGY   162 ( 2 )   927 - 939   2013.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1104/pp.113.216564

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  • Functional Characterization of a Silicon Transporter Gene Implicated in Silicon Distribution in Barley Reviewed

    Naoki Yamaji, Yukako Chiba, Namiki Mitani-Ueno, Jian Feng Ma

    PLANT PHYSIOLOGY   160 ( 3 )   1491 - 1497   2012.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1104/pp.112.204578

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  • Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene Reviewed

    Jonatan Montpetit, Julien Vivancos, Namiki Mitani-Ueno, Naoki Yamaji, Wilfried Remus-Borel, Francois Belzile, Jian Feng Ma, Richard R. Belanger

    PLANT MOLECULAR BIOLOGY   79 ( 1-2 )   35 - 46   2012.5

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    DOI： 10.1007/s11103-012-9892-3

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  • The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic Reviewed

    Namiki Mitani-Ueno, Naoki Yamaji, Fang-Jie Zhao, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   62 ( 12 )   4391 - 4398   2011.8

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    DOI： 10.1093/jxb/err158

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  • The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic Reviewed

    Namiki Mitani-Ueno, Naoki Yamaji, Fang-Jie Zhao, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   62 ( 12 )   4391 - 4398   2011.8

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    DOI： 10.1093/jxb/err158

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  • Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation. Reviewed

    Mitani N, Yamaji N, Ago Y, Iwasaki K, Ma JF

    The Plant journal : for cell and molecular biology   66 ( 2 )   231 - 240   2011.4

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  • Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation Reviewed

    Namiki Mitani, Naoki Yamaji, Yukiko Ago, Kozo Iwasaki, Jian Feng Ma

    PLANT JOURNAL   66 ( 2 )   231 - 240   2011.4

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    DOI： 10.1111/j.1365-313X.2011.04483.x

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  • Transport of silicon from roots to panicles in plants Reviewed

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani-Ueno

    Proceedings of the Japan Academy Series B: Physical and Biological Sciences   87 ( 7 )   377 - 385   2011

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  • Silicon efflux transporters isolated from two pumpkin cultivars contrasting in Si uptake Reviewed

    Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    Plant Signaling and Behavior   6 ( 7 )   73 - 76   2011

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    DOI： 10.4161/psb.6.7.15462

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  • Involvement of Silicon Influx Transporter OsNIP2;1 in Selenite Uptake in Rice Reviewed

    Xue Qiang Zhao, Namiki Mitani, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    PLANT PHYSIOLOGY   153 ( 4 )   1871 - 1877   2010.8

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    DOI： 10.1104/pp.110.157867

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  • The role of the rice aquaporin Lsi1 in arsenite efflux from roots Reviewed

    Fang-Jie Zhao, Yukiko Ago, Namiki Mitani, Ren-Ying Li, Yu-Hong Su, Naoki Yamaji, Steve P. McGrath, Jian Feng Ma

    NEW PHYTOLOGIST   186 ( 2 )   392 - 399   2010

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    DOI： 10.1111/j.1469-8137.2010.03192.x

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  • The Rice Aquaporin Lsi1 Mediates Uptake of Methylated Arsenic Species Reviewed

    Ren-Ying Li, Yukiko Ago, Wen-Ju Liu, Namiki Mitani, Joerg Feldmann, Steve P. McGrath, Jian Feng Ma, Fang-Jie Zhao

    PLANT PHYSIOLOGY   150 ( 4 )   2071 - 2080   2009.8

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    DOI： 10.1104/pp.109.140350

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  • Identification and Characterization of Maize and Barley Lsi2-Like Silicon Efflux Transporters Reveals a Distinct Silicon Uptake System from That in Rice Reviewed

    Namiki Mitani, Yukako Chiba, Naoki Yamaji, Jian Feng Ma

    PLANT CELL   21 ( 7 )   2133 - 2142   2009.7

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    DOI： 10.1105/tpc.109.067884

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  • HvLsi1 is a silicon influx transporter in barley. Reviewed

    Chiba Y, Mitani N, Yamaji N, Ma JF

    The Plant journal : for cell and molecular biology   57 ( 5 )   810 - 818   2009.3

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  • A Bacterial-Type ABC Transporter Is Involved in Aluminum Tolerance in Rice Reviewed

    Chao Feng Huang, Naoki Yamaji, Namiki Mitani, Masahiro Yano, Yoshiaki Nagamura, Jian Feng Ma

    PLANT CELL   21 ( 2 )   655 - 667   2009.2

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    DOI： 10.1105/tpc.108.064543

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  • OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice Reviewed

    Kengo Yokosho, Naoki Yamaji, Daisei Ueno, Namiki Mitani, Jian Feng Ma

    PLANT PHYSIOLOGY   149 ( 1 )   297 - 305   2009.1

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    DOI： 10.1104/pp.108.128132

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  • Identification of Maize Silicon Influx Transporters Reviewed

    Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   50 ( 1 )   5 - 12   2009.1

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    DOI： 10.1093/pcp/pcn110

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  • NIP1;1, an Aquaporin Homolog, Determines the Arsenite Sensitivity of Arabidopsis thaliana Reviewed

    Takehiro Kamiya, Mayuki Tanaka, Namiki Mitani, Jian Feng Ma, Masayoshi Maeshima, Toru Fujiwara

    JOURNAL OF BIOLOGICAL CHEMISTRY   284 ( 4 )   2114 - 2120   2009.1

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    DOI： 10.1074/jbc.M806881200

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  • Transporters of arsenite in rice and their role in arsenic accumulation in rice grain Reviewed

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Xiao-Yan Xu, Yu-Hong Su, Steve P. McGrath, Fang-Jie Zhao

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   105 ( 29 )   9931 - 9935   2008.7

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    DOI： 10.1073/pnas.0802361105

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  • Characterization of substrate specificity of a rice silicon transporter, Lsi1 Reviewed

    Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    Pflugers Archiv European Journal of Physiology   456 ( 4 )   679 - 686   2008.7

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  • Transcriptome analysis of the silicon-Magnaporthe grisea interaction

    A. M. Brunings, L. E. Datnoff, J. F. Ma, N. Mitani, Y. Nagamura, B. Rathinasabapathi

    PHYTOPATHOLOGY   98 ( 6 )   S28 - S28   2008.6

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  • Genotypic difference in silicon uptake and expression of silicon transporter genes in rice Reviewed

    Jian Feng Ma, Naoki Yamaji, Kazunori Tamai, Namiki Mitani

    PLANT PHYSIOLOGY   145 ( 3 )   919 - 924   2007.11

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    DOI： 10.1104/pp.107.107599

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  • An aluminum-activated citrate transporter in barley. Reviewed

    Jun Furukawa, Naoki Yamaji, Hua Wang, Namiki Mitani, Yoshiko Murata, Kazuhiro Sato, Maki Katsuhara, Kazuyoshi Takeda, Jian Feng Ma

    Plant & cell physiology   48 ( 8 )   1081 - 91   2007.8

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  • An efflux transporter of silicon in rice Reviewed

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Kazunori Tamai, Saeko Konishi, Toru Fujiwara, Maki Katsuhara, Masahiro Yano

    NATURE   448 ( 7150 )   209 - U12   2007.7

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    DOI： 10.1038/nature05964

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  • Characterization of silicon permeability of NIP in gramineous plants Reviewed

    Namiki Mitani, Naoki Yamaji, Maki Katsuhara, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   48   S30 - S30   2007

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  • Functional analysis of a rice Si transporter homolog Lsi6. Reviewed

    Naoki Yamaji, Namiki Mitani, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   48   S30 - S30   2007

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  • A silicon transporter in rice. Reviewed

    Ma JF, Tamai K, Yamaji N, Mitani N, Konishi S, Katsuhara M, Ishiguro M, Murata Y, Yano M

    Nature   440 ( 7084 )   688 - 691   2006.3

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  • Identification of the silicon form in xylem sap of rice (Oryza sativa L.)

    N Mitani, JF Ma, T Iwashita

    PLANT AND CELL PHYSIOLOGY   46 ( 2 )   279 - 283   2005.2

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    DOI： 10.1093/pcp/pci018

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  • Isolation and characterization of Lsi1 Reviewed

    Mitani N, Tamai K, Konishi S, Yano M, Yamaji N, Kyo M, Ma JF

    Plant and Cell Physiology   46   S82   2005

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Books

  • Rice Genomics, Genetics and Breeding

    MITANI-Ueno Namiki（ Role： Joint author ,  Transport System of Mineral Elements in Rice.）

    Springer  2018.1 

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MISC

  • イネケイ酸応答初期過程のトランスクリプトーム解析

    山地直樹, 三谷奈見季, 馬 建鋒

    日本土壌肥料学会 講演要旨集   2024.9

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  • ケイ酸応答性長距離シグナルSSS相互作用因子の探索

    三谷奈見季, 山地直樹, 小西範幸, 馬 建鋒

    日本土壌肥料学会 講演要旨集   2024.9

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  • Development of a method for collection of rice phloem sap by a DIY insect-laser device

    YAMAJI Naoki, SHINYA Tomonori, MITANI-UENO Namiki, GALIS Ivan, MA Jian Feng

    日本植物生理学会年会(Web)   65th   2024

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  • Identification of a long-distance signaling protein for regulating Si uptake in rice

    YAMAJI Naoki, MITANI-UENO Namiki, KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   64th   2023

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  • ケイ素応答性長距離シグナルの同定

    三谷奈見季, 山地直樹, 小西範幸, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   69   2023

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  • Functional characterization of two genes involved in phosphorus loading into barley grains

    HUANG Hengliang, HISANO Hiroshi, HUANG Sheng, MITANI-UENO Namiki, SATO Kazuhiro, YAMAJI Naoki, MA Jian Feng

    日本植物生理学会年会(Web)   64th   2023

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  • イネのケイ酸チャネルLsi6の変異体を用いたケイ素吸収・分配制御の詳細解析

    山地直樹, 櫻井玄, 三谷奈見季, 黄勝, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   69   2023

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  • Functional characterization of a HvSPDT-like gene in barley

    黄衡亮, 久野裕, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   69   2023

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  • 4-1-5 Identification of a gene involved in loading phosphorus into barley grains（4-1 植物の多量栄養素 2022年度東京大会）

    黄 衡亮, 久野 裕, 黄 勝, 三谷 奈見季, 佐藤 和広, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   68   37 - 37   2022.9

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    DOI： 10.20710/dohikouen.68.0_37_2

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  • P4-1-2 蛍光染色によるイネ葉のケイ素蓄積過程の解析（4-1 植物の多量栄養素 2022年度東京大会）

    山地 直樹, 藤井 理樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   68   41 - 41   2022.9

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    DOI： 10.20710/dohikouen.68.0_41_3

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  • Identification of the Si transporter Lsi3 involved in efficient xylem loading of Si in rice roots

    HUANG Sheng, YAMAJI Naoki, SAKURAI Gen, MITANI-UENO Namiki, KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   63rd   2022

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  • Regulation mechanism of boron uptake in rice

    HUANG Sheng, KONISHI Noriyuki, YAMAJI Naoki, MITANI-UENO Namiki, MA Jian Feng

    日本植物生理学会年会(Web)   63rd   2022

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  • 蛍光染色によるイネ葉のケイ素蓄積過程の解析

    山地直樹, 藤井理樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   68   2022

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  • Identification of a gene involved in loading phosphorus into barley grains

    黄衡亮, 久野裕, 黄勝, 三谷奈見季, 佐藤和広, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   68   2022

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  • 4-2-6 Role of OsLsi6 in B distribution under B-sufficient condition in rice（4-2 植物の微量栄養素 2021年度北海道大会）

    黄 勝, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   60 - 60   2021.9

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    DOI： 10.20710/dohikouen.67.0_60_3

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  • P4-1-3 イネのケイ素蓄積に関わる輸送体SIET4 の更なる解析（4-1 植物の多量栄養素 2021年度北海道大会）

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   53 - 53   2021.9

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    DOI： 10.20710/dohikouen.67.0_53_2

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  • P4-1-1 ケイ素蓄積に伴うイネ葉身のトランスクリプトーム解析（4-1 植物の多量栄養素 2021年度北海道大会）

    山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   52 - 52   2021.9

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    DOI： 10.20710/dohikouen.67.0_52_3

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  • ケイ素の輸送機構 Reviewed

    三谷奈見季, 馬 建鋒

    日本土壌肥料学雑誌   92 ( 2 )   160 - 165   2021.4

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  • イネのケイ素蓄積に関わる輸送体SIET4の更なる解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021

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  • ケイ素蓄積に伴うイネ葉身のトランスクリプトーム解析

    山地直樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021

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  • Role of OsLsi6 in B distribution under B-sufficient condition in rice

    黄勝, 山地直樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021

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  • Identification of a transporter responsible for Zn uptake in rice

    黄勝, 佐々木明正, 山地直樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   66   2020

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  • ケイ酸輸送体Lsi2の相同遺伝子SIET3,4,5の機能解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   66   2020

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  • イネのLsi2相同遺伝子の機能解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019

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  • イネにおけるケイ素輸送モデルによる輸送体配置の影響分析

    櫻井玄, 山地直樹, 三谷奈見季, 小野圭介, 横沢正幸, 酒井英光, 吉本真由美, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019

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  • イネにおける作物体内ミネラル輸送ダイナミクスの解析手法の開発

    櫻井玄, 小野圭介馬建鋒, 山地直樹, 三谷奈見季, 横沢正幸

    農業環境変動研究センター研究成果情報   2018.3

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  • イネ組織間におけるミネラル輸送

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集   63   2017

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  • P4-1-6 イネ由来ケイ酸輸送体Lsi1の更なる解析（ポスター，4-1 植物の多量栄養素，2016年度佐賀大会）

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   62 ( 0 )   52 - 52   2016

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    DOI： 10.20710/dohikouen.62.0_52_3

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  • 4-2-4 シロイヌナズナホウ酸チャネルNIP5;1の細胞膜上偏在性は効率的なホウ酸輸送に貢献する（4-2 植物の微量栄養素，2016年度佐賀大会）

    Wang Shelling, 永森 彩奈, 三谷 奈見季, 馬 建鋒, 内藤 哲, 高野 順平

    日本土壌肥料学会講演要旨集   62 ( 0 )   58 - 58   2016

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    DOI： 10.20710/dohikouen.62.0_58_1

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  • 16 イネのケイ酸吸収に関与する輸送体 Lsi1 と Lsi2 の更なる解析（関西支部講演会，2015年度各支部会）

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   62 ( 0 )   284 - 284   2016

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    DOI： 10.20710/dohikouen.62.0_284_1

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  • 膜タンパク質輸送能力の統計的逆推定プロトコルの開発

    櫻井玄, 馬建鋒, 山地直樹, 三谷奈見季

    農研機構農業環境変動研究センター成果情報(Web)   2016   2016

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  • コメへの優先的リン分配を担うイネの新規リン酸輸送体

    山地直樹, 竹本侑馬, 宮地孝明, 三谷奈見季, 吉田薫, 馬建鋒

    トランスポーター研究会年会抄録集   11th   2016

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  • 4-1-1 イネ節におけるケイ酸分配機構の統合解析(4-1 植物の多量栄養素,2015年度京都大会)

    山地 直樹, 櫻井 玄, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   61 ( 0 )   48 - 48   2015

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  • 根におけるケイ素吸収・輸送モデルの開発とイネがケイ素を多く吸収できるメカニズムの解明

    櫻井玄, 佐竹暁子, 山地直樹, 横沢正幸, FEUGIER Francois Gabriel, 三谷奈見季, 馬建鋒

    農業環境技術研究所研究成果情報   31   2015

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  • 10 イネ野生種におけるケイ酸吸収機構に関する研究(関西支部講演会,2013年度各支部会)

    三谷 奈見季, 大貝 久夫, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   60 ( 0 )   290 - 290   2014

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  • 1-5 イネにおけるケイ素吸収・輸送モデルによる植物体構造とその意義の解明(I 植物栄養と数理モデルの接点-数理モデルで植物栄養の仕組を理解する,シンポジウム,2014年度東京大会)

    櫻井 玄, 佐竹 暁子, 山地 直樹, 三谷 奈見季, 横沢 正幸, 馬 建鋒

    日本土壌肥料学会講演要旨集   60 ( 0 )   191 - 191   2014

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  • 14 イネのマンガン集積に関与する輸送体OsMTP8の解析(関西支部講演会,2013年度各支部会)

    竹本 侑馬, 三谷 奈見季, 佐々木 明正, 山地 直樹, 馬 建鋒, 加藤 伸一郎, 岩崎 貢三, 上野 大勢

    日本土壌肥料学会講演要旨集   60 ( 0 )   291 - 291   2014

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  • シロイヌナズナ熱活性型レトロトランスポゾンの転写活性とサイレンシング

    松永航, 増田ゆかり, 三谷奈見季, 馬建鋒, 加藤敦之, 伊藤秀臣

    日本遺伝学会大会プログラム・予稿集   86th   2014

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  • イネの節で局在する輸送体OsZIP3は亜鉛の優先的分配に関与する

    佐々木明正, 山地直樹, 柏野美帆, 三谷奈見季, 馬建鋒

    日本植物生理学会年会要旨集   55th   2014

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  • イネのケイ素輸送におけるカスパリー線の役割とトランスポーター配置

    櫻井玄, 馬建鋒, 佐竹暁子, 横沢正幸, 山地直樹, 三谷奈見季

    日本植物生理学会年会要旨集   55th   2014

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  • 4-1-2 野生イネ由来ケイ酸トランスポーターの解析(4-1 植物の多量栄養素)

    三谷 奈見季, 大貝 久生, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   59 ( 0 )   54 - 54   2013

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  • イネの節におけるミネラルの維管束間輸送と分配制御機構

    山地直樹, 佐々木明正, 鄭録慶, 夏継星, 横正健剛, 三谷奈見季, 馬建鋒

    トランスポーター研究会年会抄録集   8th   47   2013

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  • イネ由来ケイ酸輸送体Lsi1の発現応答およびその制御機構の解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   54th   2013

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  • P9-3 ケイ酸輸送体Lsi1のケイ酸応答性および組織局在性を制御するプロモーターの解析(9.植物の多量栄養素,2012年度鳥取大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   58 ( 0 )   59 - 59   2012

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  • 11-38 イネ亜ヒ酸排出能の品種間差(11.植物の有害元素)

    大貝 久生, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   57 ( 0 )   91 - 91   2011

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  • 9-28 ケイ酸輸送体Lsi1の発現制御機構の解析(9.植物の多量栄養素)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   57 ( 0 )   67 - 67   2011

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  • 9-15 オオムギ低親和性硝酸トランスポーターの機能解析(9.植物の多量栄養素)

    石川 伸二, 園部 和幸, 三谷 奈見季, 山地 直樹, 馬 建鋒, 大山 卓爾, 末吉 邦

    日本土壌肥料学会講演要旨集   57 ( 0 )   62 - 62   2011

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  • 9-3 イネケイ酸輸送体遺伝子Lsi1を過剰発現したシロイヌナズナの解析(9.植物の多量栄養素,2010年度北海道大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   56 ( 0 )   54 - 54   2010

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  • 9-4 イネケイ酸輸送体Lsi3の解析(9.植物の多量栄養素,2010年度北海道大会)

    山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   56 ( 0 )   55 - 55   2010

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  • イネの新規ケイ酸輸送体Lsi3の解析

    山地直樹, 三谷奈見季, 馬建鋒

    日本植物生理学会年会要旨集   51st   2010

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  • オオムギのケイ素分配に関与するトランスポーターの解析

    馬建鋒, 山地直樹, 千葉由佳子, 三谷奈見季

    日本植物生理学会年会要旨集   51st   2010

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  • オオムギとイネのケイ酸吸収機構の違いに関する研究

    三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   51st   2010

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  • 9-20 ブルームレスキュウリ用カボチャ台木のケイ酸吸収特性のさらなる解析(9.植物の多量栄養素,2009年度京都大会)

    馬 建鋒, 吾郷 幸子, 山地 直樹, 三谷 奈見季, 岩崎 貢三

    日本土壌肥料学会講演要旨集   55 ( 0 )   71 - 71   2009

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  • 22 カボチャ及びトマトのケイ酸吸収特性と関連遺伝子の単離(関西支部講演会,2008年度各支部会)

    馬 建鋒, 吾郷 幸子, 山地 直樹, 三谷 奈見季, 岩崎 貢三

    日本土壌肥料学会講演要旨集   55 ( 0 )   298 - 298   2009

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  • Identification of an aquaporin involved in arsenite transport and tolerance.

    Kamiya Takehiro, Tanaka Mayuki, Mitani Namiki, Ma Jian Feng, Maeshima Masayoshi, Fujiwara Toru

    Plant and Cell Physiology Supplement   2009 ( 0 )   S0033 - S0033   2009

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    To identify molecule(s) which mediates As(III) uptake in plants, we screened for As(III) tolerant mutants expecting that loss-of-function mutant of As(III) transporter would be tolerant to As(III). After screening of EMS-mutagenized Arabidopsis thaliana M2 seeds, we identified 3 independent mutants. We also analyzed tolerance of T-DNA mutants of NIPs, which are homologues of aquaglyceroporins. Among the lines tested, nip1;1 mutant showed As(III) tolerance. Sequencing of the NIP1;1 in the mutants revealed that each mutants had an independent mutation in NIP1;1, establishing that NIP1;1 is the causal gene of As(III) tolerance. Xenopus oocytes injected with NIP1;1 cRNA accumulated much As(III) than control oocytes injected with water. The promoter-GUS and real-time PCR analysis showed that NIP1;1 was highly expressed in roots. From these data, we conclude that NIP1;1 is the major As(III) transporter involved in As(III) uptake in roots in A. thaliana.

    DOI： 10.14841/jspp.2009.0.S0033.0

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  • Differential gene expression of rice in response to silicon and rice blast fungus Magnaporthe oryzae

    A. M. Brunings, L. E. Datnoff, J. F. Ma, N. Mitani, Y. Nagamura, B. Rathinasabapathi, M. Kirst

    ANNALS OF APPLIED BIOLOGY   155 ( 2 )   161 - 170   2009

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  • オオムギ由来排出型ケイ酸輸送体HvLsi2の更なる機能解析

    三谷奈見季, 山地直樹, 千葉由佳子, 馬建鋒

    日本植物生理学会年会要旨集   50th   2009

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  • P9-30 カボチャ2品種のケイ酸吸収特性の解析(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)

    吾郷 幸子, 三谷 奈見季, 山地 直樹, 岩崎 貢三, 馬 建鋒

    日本土壌肥料学会講演要旨集   54 ( 0 )   95 - 95   2008

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  • P9-31 異なるイネ科植物由来のケイ酸トランスポーター特性の比較解析(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)

    三谷 奈見季, 千葉 由佳子, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   54 ( 0 )   95 - 95   2008

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  • 9-23 植物の金属ストレスにおけるMATE遺伝子の機能解析(9.植物の無機栄養,2008年度愛知大会)

    横正 健剛, 上野 大勢, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   54 ( 0 )   77 - 77   2008

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  • 9-26 イネのヒ素吸収に関与するトランスポーターの同定(9.植物の無機栄養,2008年度愛知大会)

    馬 建鋒, 山地 直樹, 三谷 奈見季, Zhao Fangjie

    日本土壌肥料学会講演要旨集   54 ( 0 )   78 - 78   2008

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  • Functional analysis of OsFRDL1, a citrate transporter involved in Fe translocation in rice

    Yokosho Kengo, Ueno Daisei, Yamaji Naoki, Mitani Namiki, Ma Jian Feng

    Plant and Cell Physiology Supplement   2008 ( 0 )   27 - 27   2008

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    We recently identified an Al-tolerant gene (HvAACT1) from barley, which encodes an Al-activated citrate transporter. Here, we report the closest homolog of this gene in rice (OsFRDL1). We compared the Al-induced citrate secretion between the wild-type rice and Tos-17 insertion lines. However, there was no difference in the citrate secretion between these lines. When these lines were grown in a nutrient solution with low Fe concentration, chlorosis on the new leaves was observed only in the knocknot lines. The concentration of Fe and citrate in the xylem sap was much lower and Fe precipitation in the root stele was observed in the knockout line. The protein encoded by this gene showed transport activity for citrate. This gene was expressed in the roots and the expression level was not affected by Fe nutrition. These results suggest that OsFRDL1 is a citrate transporter, which is necessary for Fe translocation to the shoot.

    DOI： 10.14841/jspp.2008.0.0027.0

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  • オオムギにおけるケイ酸トランスポーターの解析

    千葉由佳子, 三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   49th   2008

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  • イネのヒ素吸収におけるケイ酸輸送体の関与

    山地直樹, 三谷奈見季, XU Xiao-Yan, MCGRATH Steve P., ZHAO Fang-Jie, 馬建鋒

    日本植物生理学会年会要旨集   49th   2008

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  • イネのアルミニウム耐性に関与する細菌タイプABCトランスポーターの解析

    馬建鋒, 山地直樹, 黄朝鋒, 三谷奈見季

    日本植物生理学会年会要旨集   49th   2008

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  • トウモロコシのケイ酸トランスポーターの同定

    三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   49th   2008

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  • Silicon transporters in rice

    馬 建鋒, 山地 直樹, 三谷 奈見季

    Protein, nucleic acid and enzyme   52 ( 14 )   1849 - 1856   2007.11

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  • P9-40 大麦におけるケイ酸吸収機構の解析(9.植物の無機栄養,2007年度東京大会)

    千葉 由佳子, 三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   89 - 89   2007

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  • 9-41 トウモロコシ由来のケイ酸吸収遺伝子の単離と解析(9.植物の無機栄養,2007年度東京大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   76 - 76   2007

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  • 9-6 オオムギにおけるアルミニウムによるクエン酸分泌に関与する遺伝子の単離と機能解析(9.植物の無機栄養,2007年度東京大会)

    古川 純, 山地 直樹, 王 華, 三谷 奈見季, 村田 佳子, 佐藤 和広, 且原 真木, 武田 和義, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   64 - 64   2007

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  • P9-41 イネのケイ酸輸送体と吸収・集積機構(9.植物の無機栄養,2007年度東京大会)

    山地 直樹, 三谷 奈見季, 玉井 一規, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   90 - 90   2007

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  • イネケイ酸輸送体Lsi6の機能解析

    山地直樹, 三谷奈見季, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007

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  • イネ科NIPのケイ酸輸送特性の解析

    三谷奈見季, 山地直樹, 且原真木, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007

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  • 9-66 イネケイ酸吸収遺伝子Lsi1の輸送特性の解析(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

    三谷 奈見季, 且原 真木, 馬 建鋒

    日本土壌肥料学会講演要旨集   52 ( 0 )   72 - 72   2006

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  • Functional characterization of a Si transporter gene Lsi1 in rice

    N Mitani, N Yamaji, K Tamai, S Konishi, M Yano, JF Ma

    PLANT AND CELL PHYSIOLOGY   47 ( 0 )   S61 - S61   2006

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  • 9-67 イネケイ酸吸収関連遺伝子lsi2の解析(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

    玉井 一規, 山地 直樹, 三谷 奈見季, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   52 ( 0 )   73 - 73   2006

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  • イネのケイ酸輸送体.

    馬 建鋒, 山地直樹, 三谷奈見季, 玉井一規

    細胞工学   25:778-779   2006

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  • 9-26 イネケイ酸吸収関連遺伝子Lsi1の機能解析(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)

    三谷 奈見季, 山地 直樹, 玉井 一規, 小西 左江子, 矢野 昌裕, 村田 佳子, 馬 建鋒

    日本土壌肥料学会講演要旨集   51 ( 0 )   66 - 66   2005

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  • 9-25 新規イネケイ酸吸収関連遺伝子Lsi2の単離と解析(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)

    玉井 一規, 三谷 奈見季, 山地 直樹, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   51 ( 0 )   2005

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  • Uptake system of silicon in different plant species

    MITANI N.

    J. Exp. Bot.   56   1255 - 1261   2005

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  • Characterization of the silicon uptake system and molecular mapping of the silicon transporter gene in rice

    JF Ma, N Mitani, S Nagao, S Konishi, K Tamai, T Iwashita, M Yano

    PLANT PHYSIOLOGY   136 ( 2 )   3284 - 3289   2004.10

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  • Characterization of a low Si mutant and molecular mapping of responsible gene in rice

    K Tamai, N Mitani, S Nagao, S Konishi, Y Takeoka, M Yano, JF Ma

    PLANT AND CELL PHYSIOLOGY   45   S49 - S49   2004

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  • 異なる植物におけるケイ酸吸収及び輸送機構に関する解析(9. 植物の無機栄養, 2004年度大会講演要旨集)

    三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   50 ( 0 )   93 - 93   2004

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  • 新規低ケイ酸含量イネ突然変異体 (LSi2) の単離と解析(9. 植物の無機栄養, 2004年度大会講演要旨集)

    武岡 祐子, 三谷 奈見季, 玉井 一規, 佐藤 光, 藤原 徹, 馬 鋒建

    日本土壌肥料学会講演要旨集   50 ( 0 )   92 - 92   2004

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  • イネにおけるケイ酸輸送形態の同定(関西支部講演会, 日本土壌肥料学会支部講演会講演要旨集2004年度)

    三谷 奈見季, 岩下 孝, 馬 建鋒

    日本土壌肥料学会講演要旨集   50 ( 0 )   301 - 301   2004

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  • イネ低ケイ酸含量突然変異体 (lsi1) の解析と原因遺伝子のマッピング(9. 植物の無機栄養, 2004年度大会講演要旨集)

    玉井 一規, 三谷 奈見季, 長尾 咲子, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   50 ( 0 )   93 - 93   2004

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  • 9-68 異なる植物によるケイ酸吸収機構の解析(9.植物の無機栄養)

    三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   49 ( 0 )   87 - 87   2003

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    Language：Japanese   Publisher：一般社団法人 日本土壌肥料学会  

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  • 9. 植物の無機栄養 異なる植物によるケイ酸吸収機構の解析

    三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集   49   2003

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Presentations

  • Identification of a gene involved in loading phosphorus into barley grains

    2022.9.13 

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    Event date： 2022.9.13 - 2022.9.15

    Presentation type：Oral presentation (general)  

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  • 蛍光染色によるイネ葉のケイ素蓄積過程の解析

    山地直樹, 藤井理樹, 三谷奈見季, 馬 建鋒

    日本土壌肥料学会2022年度東京大会  2022.9 

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    Event date： 2022.9.13 - 2022.9.15

    Presentation type：Oral presentation (general)  

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  • イネのSIET4輸送体は細胞特異的ケイ素蓄積に関与する

    三谷奈見季, 山地直樹, 吉岡佑真, 宮地孝明, 馬 建鋒

    第63回日本植物生理学会年会（つくば）  2022.3.22 

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    Event date： 2022.3.22 - 2022.3.24

    Presentation type：Poster presentation  

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  • Node-localized transporters of Phosphorus Essential for Seed Development in Rice. Invited

    Che, J, Yamaji, N, Miyaji, T, Mitani-Ueno, N, Kato, Y, Shen, R. F, and Ma, J. F

    2022.3 

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    Event date： 2022.3.22 - 2022.3.24

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  • イネの根における効率的なケイ酸ローディングに寄与する輸送体Lsi3

    黄 勝, 山地直樹, 櫻井 玄, 三谷奈見季, 小西範幸, 馬 建鋒

    第63回日本植物生理学会年会（つくば）  2022.3 

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    Event date： 2022.3.22 - 2022.3.24

    Presentation type：Oral presentation (general)  

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  • Regulation mechanism of boron uptake in rice.

    Huang, S, Konishi, N, Yamaji, N, Mitani-Ueno, N, Ma, J. F

    2022.3 

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    Event date： 2022.3.22 - 2022.3.24

    Presentation type：Oral presentation (general)  

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  • Role of OsLsi6 in B distribution under B-sufficient condition in rice.

    Huang, S, Yamaji N, Mitani N, Ma JF

    2021.9 

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    Event date： 2021.9.14 - 2021.9.16

    Language：English   Presentation type：Oral presentation (general)  

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  • イネのケイ素蓄積に関わる輸送体SIET4の更なる解析

    三谷奈見季, 山地直樹, 馬 建鋒

    日本土壌肥料学会2021年度北海道大会  2021.9 

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    Event date： 2021.9.14 - 2021.9.16

    Presentation type：Poster presentation  

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  • ケイ素蓄積に伴うイネ葉身のトランスクリプトーム解析

    山地直樹, 三谷奈見季, 馬 建鋒

    日本土壌肥料学会2021年度北海道大会  2021.9 

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    Event date： 2021.9.14 - 2021.9.16

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  • Plasma membrane H+-ATPase is required for active Si uptake in rice.

    Yamamoto, S, Kawai, Y, Okumura, M, You, T, Mitani-Ueno, N, Yamaji, N, Ma, J. F, Kinoshita, T

    2020.3 

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    Event date： 2020.3.19 - 2020.3.21

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  • Identification of a transporter for preferential distribution of phosphorus in Arabidopsis.

    Ding, G, Lei, G. J, Yamaji, N, Yokosho, K, Mitani-Ueno, N, Huang, S, nd Ma, J. F

    2020.3 

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    Event date： 2020.3.19 - 2020.3.21

    Presentation type：Poster presentation  

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  • Integrated micro-scale;macro-scale modeling of silicon transportation system in rice

    Sakurai, G, Yamaji, N, Mitani-Ueno, N, Yokozawa, M, Ono, K, d Ma, J. F

    2019.3 

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    Event date： 2019.3.13 - 2019.3.15

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  • Structure of a silicon transporter in plant. The 60th Annual Meeting of the Japanese Society of Plant Physiologists, Symposium on ‘Plant mineral transporters: from function to structure and modelling’

    Saitoh, Y, Matsuki, K, Yonekura, S, Yang, L, Mitani-Ueno, N, Yamaji, N, Shen, J, Ma, J. F, Suga, M

    2019.3 

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    Event date： 2019.3.13 - 2019.3.15

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  • イネのケイ酸チャネルLsi6の変異体を用いたケイ素吸収・分配制御の詳細解析

    山地直樹, 櫻井 玄, 三谷奈見季, 黄 勝, 馬 建鋒

    日本土壌肥料学会2023年度愛媛大会  2023.9 

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  • Functional characterization of a HvSPDT-like gene in barley

    2023.9 

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  • ケイ素応答性長距離シグナルの同定

    三谷奈見季, 山地直樹, 小西範幸, 馬 建鋒

    日本土壌肥料学会2023年度愛媛大会  2023.9 

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  • イネのケイ素吸収を制御する長距離シグナルタンパク質の同定

    山地直樹, 三谷奈見季, 小西範幸, 馬 建鋒

    第64回日本植物生理学会年会  2023.3 

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  • Functional characterization of two genes involved in phosphorus loading into barley grains.

    Huang, H, Hisano, H, Huang, S, Mitani-Ueno, N, Sato, K, Yamaji, N, Ma, J. F

    2023.3 

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  • イネのLsi2相同遺伝子の機能解析

    三谷 奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会2019年度静岡大会  2019.9.5 

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  • Identification and functional analysis of transporter genes involved in phosphorus redistribution in rice.

    Mitani-Ueno, N, Yamaji, N, Ma, J. F

    2019.3.15 

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  • A novel phosphate transporter controlling grain P accumulation in rice.

    XVIII International Plant Nutrition Colloquium 2017  2017 

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  • ホウ酸チャネルNIP5;1の細胞膜内偏在はリン酸に依存しホウ酸の効率的な吸収に寄与する．

    第57回日本植物生理学会年会  2016 

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  • イネ節の新規リン酸輸送体と少燐米の可能性．

    イネ 遺伝学・分子生物学ワークショップ2016  2016 

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  • イネ由来ケイ酸輸送体Lsi1の更なる解析．

    日本土壌肥料学会2016年度大会  2016 

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  • OsSultr3;4 localized in rice node is responsible for preferential distribution of Pi to new leaves and grains.

    The 17th International Workshop on Plant Membrane Biology (IWPMB2016)  2016 

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  • コメへの優先的リン分配を担うイネの新規リン酸輸送体．

    第11回トランスポーター研究会年会，第316回生物圏シンポジウム  2016 

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  • イネ節におけるケイ酸分配機構の統合解析．

    日本土壌肥料学会2015年度京都大会  2015 

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  • A co-operated transport system for mineral element uptake in rice roots.

    International Society of Root Research 9th Symposium.  2015 

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  • Phosphorylation-mediated polar localization of a boric acid channel is required for efficient uptake of boron in Arabidopsis thaliana.

    13th International Conference on the Biogeochemistry of Trace Elements.  2015 

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  • イネにおけるケイ素吸収・輸送モデルによる植物体構造とその意義の解明．

    日本土壌肥料学会2014年度東京大会  2014 

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  • Transcriptional regulation of Si transporter genes involved in uptake and distribution in rice.

    6th International Conference on Silicon in Agriculture.  2014 

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  • イネのケイ素輸送におけるカスパリー線の役割とトランスポーター配置．

    第55回日本植物生理学会年会  2014 

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  • Silicon transporters and their role in plants.

    6th International Conference on Silicon in Agriculture.  2014 

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  • イネの節で局在する輸送体OsZIP3は亜鉛の優先的分配に関与する．

    第55回日本植物生理学会年会  2014 

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  • The role of Casparian strips and Lsi transpoter distribution in efficient Si transport in rice.

    6th International Conference on Silicon in Agriculture.  2014 

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  • Mineral transport from soil to seed.

    XVII International Plant Nutrition Colloquium 2013  2013 

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  • イネ由来ケイ酸輸送体Lsi1の発現応答およびその制御機構の解析．

    第54回日本植物生理学会年会  2013 

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  • Promoter analysis of a rice influx silicon transporter gene Lsi1.

    16th International Workshop on Plant Membrane Biology.  2013 

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  • イネ節の維管束間輸送に働くケイ酸輸送体．

    第54回日本植物生理学会年会  2013 

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  • イネのマンガン集積に関与する輸送体OsMTP8の解析．

    第109回日本土壌肥料学会関西支部講演会  2013 

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  • イネのマンガンホメオスタシスに関与する輸送体の解析．

    日本土壌肥料学会年会  2013 

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  • イネ野生種におけるケイ酸吸収機構に関する研究．

    第109回日本土壌肥料学会関西支部講演会  2013 

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  • Role of node-located transporters in mineral distribution in rice.

    XVII International Plant Nutrition Colloquium 2013  2013 

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  • 野生イネ由来ケイ酸トランスポーターの解析．

    日本土壌肥料学会年会  2013 

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  • ケイ酸輸送体Lsi1のケイ酸応答性および組織局在性を制御するプロモーターの解析．

    日本土壌肥料学会年会  2012 

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  • A gene encoding a cysteine-rich peptide is involved in rice Al tolerance.

    The 8th International Symposium on Plant-Soil Interactions at Low pH.  2012 

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  • Preferential Si distribution to the panicles mediated by transporters for inter-vascular transfer at rice node.

    3rd Japan-China Joint Workshop on Plant Nutrition.  2011 

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  • OsCDT3 encoding a cysteine rich peptide is involved in rice Al tolerance.

    International Symposium "Strategies of Plants against Global Environmental Change".  2011 

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  • Transporters involved in preferential distribution of Si to the panicles at the node in rice

    5th International Conference on Silicon in Agriculture  2011 

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  • Isolation and functional analysis of Si transporters in two pumpkin cultivars contrasting in Si uptake.

    5th International Conference on Silicon in Agriculture.  2011 

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  • イネ亜ヒ酸排出能の品種間差．

    日本土壌肥料学会年会  2011 

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  • Transport of silicon from roots to panicles in different plant species

    5th International Conference on Silicon in Agriculture  2011 

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  • ケイ酸輸送体Lsi1の発現制御機構の解析．

    日本土壌肥料学会年会  2011 

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  • ケイ酸トランスポーターLsi1は亜ヒ酸も亜セレン酸も輸送する．

    第３回植物アクアポリン研究検討会  2011 

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  • The biology of silicon.

    The 4th Workshop on the Aqueous Chemistry and Biochemistry of Silicon.  2011 

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  • Isolation and functional characterization of silicon transporters in two pumpkin cultivars contrasting in silicon accumulation.

    3rd Japan-China Joint Workshop on Plant Nutrition.  2011 

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  • イネケイ酸輸送体OsNIP2;1の基質選択性の解析

    第３回植物アクアポリン研究検討会  2011 

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  • オオムギとイネのケイ酸吸収機構の違いに関する研究

    第51回日本植物生理学会年会  2010 

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  • Functional analysis of three MATE genes encoding citrate transporter in rice.

    Plant Membrane Biology, 15th International Workshop Adelaide 2011  2010 

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  • イネケイ酸輸送体Lsi3の解析

    日本土壌肥料学会年会  2010 

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  • Transporters involved in inter-vascular transfer of Si at the node of rice.

    Plant Membrane Biology, 15th International Workshop Adelaide 2010  2010 

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  • イネケイ酸輸送体遺伝子Lsi1を過剰発現したシロイヌナズナの解析

    日本土壌肥料学会年会  2010 

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  • イネの新規ケイ酸輸送体Lsi3の解析

    第51回日本植物生理学会年会  2010 

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Research Projects

  • イネのケイ素集積ロジスティクス

    Grant number：24H00571  2024.04 - 2028.03

    日本学術振興会  科学研究費助成事業  基盤研究(A)

    山地 直樹, 櫻井 玄, 三谷 奈見季, 小西 範幸

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    Grant amount：\48360000 （ Direct expense: \37200000 、 Indirect expense：\11160000 ）

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  • ケイ素応答性長距離シグナルによるミネラル恒常性維持機構の実態解明

    Grant number：24K08648  2024.04 - 2027.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    三谷 奈見季

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    Grant amount：\4550000 （ Direct expense: \3500000 、 Indirect expense：\1050000 ）

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  • Molecular basis and substrate selectivity of toxic metalloid channels and transporters

    Grant number：23H02450  2023.04 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    菅 倫寛, 三谷 奈見季

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    Grant amount：\18720000 （ Direct expense: \14400000 、 Indirect expense：\4320000 ）

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  • Molecular Basis and substrate selectivity of toxic metalloid Channels and Transporters

    Grant number：23K27143  2023.04 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    菅 倫寛, 三谷 奈見季

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    Grant amount：\18720000 （ Direct expense: \14400000 、 Indirect expense：\4320000 ）

    ケイ酸チャネルLsi1はすでに結晶構造を報告しているが、岡山大に導入されたクライオ電子顕微鏡で溶液条件と凍結条件を検討して、構造解析に成功した。その結果、結晶構造とクライオ電顕構造とで共通した部分が多かったが、基質選択フィルターを構成する水分子の数や、チャネル入口に違いが見つかった。今後は電顕試料の条件を改善して、分解能を向上させ、これらの構造の違いによる生理機能への役割を明らかにする。また、クライオ電顕を用いて金属輸送体の立体構造を高分解能で解析することにも成功した。すなわち、1種類の金属輸送体について、基質の結合していない状態、基質が結合した状態、別の基質が結合した状態の３つの立体構造を比較して、基質の選択に重要なアミノ酸残基の候補を明らかにしている。また、クライオ電子顕微鏡を用いて別の金属輸送体と半金属チャネルの立体構造をそれぞれ決定した。立体構造から基質選択に重要なアミノ酸残基の候補を絞り込んだ。
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    また、結晶構造とクライオ電顕構造に基づいた変異体を作成し、それら変異体の基質輸送活性をアフリカツメガエル卵母細胞発現系により測定した。

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  • Plasticity of mineral element transport system in response to soil environmental fluctuations in plants

    Grant number：21H05034  2021.07 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

    馬 建鋒, 山地 直樹, 宮地 孝明, 三谷 奈見季, 菅 倫寛

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    Grant amount：\189280000 （ Direct expense: \145600000 、 Indirect expense：\43680000 ）

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  • イネ科作物の有害ミネラル集積機構の解明

    Grant number：21H04716  2021.04 - 2025.03

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    馬 建鋒, 山地 直樹, 三谷 奈見季

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    Grant amount：\41860000 （ Direct expense: \32200000 、 Indirect expense：\9660000 ）

    １．イネカドミウム集積とヒ素集積 QTL 遺伝子のマッピング
    イネのカドミウム品種間差を利用して作出したマッピング集団を用いて、新しいマーカーを作成しながら、染色体7番と11番のQTL遺伝子の候補領域を狭めることができた。7番染色体にあるQTL遺伝子についてプロモーター領域配列の比較、発現パータンの比較などを行い、この遺伝子の発現の違いがカドミウム集積に寄与している可能性を示した。またカドミウム集積における側根と根毛の役割を解析し、根毛ではなく、側根がカドミウムの吸収に大きな役割を果たしていることを明らかにした。
    ヒ素集積に関して、6番染色体にあるヒ素集積に関与するQTL遺伝子の候補領域を350kbまで狭めることができた。
    2．オオムギのカドミウム集積QTL遺伝子のマッピングとヒ素集積の品種間差
    オオムギのカドミウム集積の品種間差を利用して、4番染色体にあるカドミウム集積に関与するQTL遺伝子のマッピングを行った。いくつかのマーカーを新たに設計し、候補領域を8375kbまで狭めることができた。
    またオオムギヒ素集積の品種間差を調べるために、コアコレクションから100品種を選んで、圃場栽培された種子とわら中のヒ素濃度を測定し、比較した。その結果、ヒ素集積において大きな品種間差があることが分かった。また水耕栽培で幼苗のヒ素集積も比較し、種子のヒ素集積と必ず一致しないことを明らかにした。いくつか集積が大きく異なる品種を選んで、関連遺伝子をマッピングするための交配を行った。

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  • Integrated analysis of mineral ion dynamics in rice crop exposed to acute herbivory stress

    Grant number：21H02196  2021.04 - 2024.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    Ivan Galis, 網干 貴子, 三谷 奈見季

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    Grant amount：\17550000 （ Direct expense: \13500000 、 Indirect expense：\4050000 ）

    First year of the project was fully devoted to collection of large scale data for further analyses. Two large scale herbivory experiments have been completed in summer season of FY2021. Two herbivore types (chewing insect larvae and sucking insect adults) were used as proposed after implementing hydroponic rice plant cultivation system. Five tissues (young leaf, attacked leaf, old leaves, stem, and root) over time course of three days have been collected. Out of collected samples, all originally proposed analyses have been carried out; in particular, extensive mineral analysis from 440 rice samples by ICP-MS, phytohormones, and secondary metabolites by LC-MS/MS were performed at IPSR (Galis, Mitani). Sumigraph analysis of total nitrogen and carbon from 440 samples was performed at Yamagata University (Aboshi). Large scale RNA sequencing (RNAseq) of 110 pooled replicated samples was outsourced and analyzed data obtained in March 2022. In outcome, several mineral elements showed differential contents in herbivore attacked plants. Furthermore, large increases in nitrogen containing alkaloids (p-coumaroylputrescine, feruloylputrescine, isopentylamine) were found in the attacked leaves. Jasmonic acid was the main phytohormone elicited in locally attacked leaves. A large number of genes related to defense was elicited in locally attacked leaves but many genes for mineral transport have also been observed in the regulated gene set. Overall, a very large amount of data was obtained that clearly depict extensive changes in rice metabolism in response to herbivore attack.

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  • 低投入を目指したイネ体内におけるミネラル再分配機構の分子生物学的解析

    Grant number：20K05773  2020.04 - 2023.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    三谷 奈見季

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    Grant amount：\4290000 （ Direct expense: \3300000 、 Indirect expense：\990000 ）

    本年度は、維管束組織で高発現する二つ目の輸送体、SIETについての実験を行った。SIETはケイ酸輸送体Lsi2と比較的相同性の高い輸送体であり、イネにはSIET2-5の4つがある。このうち維管束組織に高発現するのはSIET5である。定量PCRの結果SIET5は生育期間を通して地上部とくに葉鞘で高発現していた。タマネギの表皮細胞やイネプロトプラストを用いた系では細胞内局在がはっきりしなかったことから、抗SIET5抗体を作製し免疫組織染色を行った。その結果SIET5は維管束鞘細胞の細胞膜に局在していることが明らかになった。
    次にゲノム編集技術を用い、変異体の作出を行った。SIETがケイ酸輸送体のホモログであることならびに、主な発現場所が地上部であることを鑑み、地上部のケイ酸の分配に関する表現型の解析を行った。その結果、葉鞘においてケイ素の濃度が野生型より高く、逆に葉身においては低い傾向が見られたが他のミネラルには野生型と遺伝子破壊株の間に差が見られなかった。
    さらにこのSIET5と相同性の高いSIET4の解析も行っている。SIET4はケイ酸の排出輸送活性を持ち、地上部で恒常的に発現していることがあきらかになった。siet4変異体についてはすでに作成済みのため、次年度はその解析を行い、機能を明らかにする。

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  • イネの環境応答統合メカニズムにおける節の役割

    Grant number：19H03250  2019.04 - 2023.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    山地 直樹, 三谷 奈見季, 横正 健剛

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    Grant amount：\17290000 （ Direct expense: \13300000 、 Indirect expense：\3990000 ）

    イネ科植物は「節」を構成単位とするモジュール化された体制を採用しており、節には栄養素の分配や成長/休眠の制御などの機能が高度に集約されていることから、個体レベルの環境応答においても様々なシグナルの受発信/変換/転送を担う主要な情報処理の場であると考えられる。
    本年度は、節の環境応答に関与する遺伝子を見出すため、出穂期のイネに対し、短期的なストレス処理を行った最上位節(節I)と下位節(節V~VI)の次世代シーケンサーを用いたRNA-seq解析を実施した。各処理区で発現が有意に変動した転写調節因子、受容体、ペプチドホルモン、などをコードする数十の機能未知遺伝子を選抜した(一次選抜)。さらに定量的RT- PCRによるより詳細な遺伝子発現解析を行い、既存の発現データベース等の情報も加味して検討した結果、節において他の器官よりも特に発現レベルが高く、いずれかのストレス処理に顕著に応答する13の遺伝子を選抜した(二次選抜)。これらの遺伝子についてCRISPR/Cas9法による遺伝子破壊株の作出を進めている。
    イネ節の肥大維管束木部転送細胞に極めて高発現し、ケイ酸の分配を制御する輸送体Lsi6の遺伝子発現を制御する転写調節因子の探索については、酵母one-hybridシステムによるスクリーニングを計画していたが、Lsi6プロモーターの候補領域が酵母において著しい偽陽性を生じることが判明した。
    イネのアルミニウム耐性遺伝子でバクテリア型ABC輸送体のサブユニットをコードするSTAR1については節の維管束系でも高発現していることなどが確認できた。

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  • Study on the regulation mechanism of transformation amenability in barley

    Grant number：19H02930  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    HISANO Hiroshi

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    Grant amount：\17550000 （ Direct expense: \13500000 、 Indirect expense：\4050000 ）

    In order to fine map of the three TFA loci involved in the efficiency of genetic transformation of barley, the genetic experiments were conducted using two populations derived from a cross between transformable and non-transformable cultivars. The results revealed the presence of genes contributing to callus induction and differentiation in a region called TFA3. In addition, RNA-seq analysis revealed genes with differential expression between transformable and non-transformable cultivars. One gene with significant expression was cloned and introduced into barley, but no improvement in callus induction efficiency was observed. On the other hand, a genome editing experiment using a TFA-selected barley line succeeded in obtaining individuals with mutations in the target gene.

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  • Identification and functional analysis of transporter genes involved in phosphorus recycling in rice

    Grant number：19K21145  2019.04 - 2020.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up  Grant-in-Aid for Research Activity Start-up

    MITANI-UENO Namiki

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    Grant amount：\2990000 （ Direct expense: \2300000 、 Indirect expense：\690000 ）

    Phosphorus (P) able to be redistributed from old to young organs for internal recycling of P, especially under P-limited condition. To identify the transporters that are involved in the P recycling in rice, we performed transcriptomic analysis of vascular bundle tissues from old and young leaves of rice grown under nutrient deficiency condition. Among genes highly expressed in the vascular bundles of old leaves, the protein encoded by OsSultr3;3 showed the P transport activity. This genes localized at the plasma membrane of leaf tissues. Knock out of this genes resulted in P accumulation in old leaves. These results indicated that OsSultr3;3 might be involved in the redistribution of P from source to sink organs.

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  • Integrated analysis of mineral transport system in crops

    Grant number：16H06296  2016.04 - 2021.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Specially Promoted Research  Grant-in-Aid for Specially Promoted Research

    Ma Jian Feng

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    Grant amount：\536250000 （ Direct expense: \412500000 、 Indirect expense：\123750000 ）

    We have identified more than 30 transporter genes related to uptake, translocation and distribution of mineral elements including essential, beneficial and toxic elements mainly in rice and buckwheat. We further revealed the mechanisms for response of these transporters to environmental changes, regulation of transporters and their roles in plant growth and productivity by using different approaches. For the first time, we succeeded to crystalize the silicon transporter Lsi1 and revealed its crystal structure. We also constructed a mathematical model for mineral element transport at whole plant scales. These achievements have been published in many international top journals including Nature.

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  • Soil-pH adaptivity trade-off in gramineous plants

    Grant number：16K14871  2016.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    Yamaji Naoki, Ma Jian Feng, Mitani Namiki, Yokosho Kengo

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    Grant amount：\3770000 （ Direct expense: \2900000 、 Indirect expense：\870000 ）

    Soil pH has a large impact on availability of mineral elements. For example, at a low pH, Al is solubilized to inhibit plant growth, while at a high pH, Fe availability becomes very low. However, different plant species show different adaptability to soil pH. Among cereal crops, rice is highly tolerant to Al but relatively sensitive to Fe-deficiency. By contrast, barley is the most sensitive to Al but highly tolerant to Fe-deficiency, while rye is able to adapt to a wide range of soil pHs. However, the molecular mechanisms underlying these differences are poorly understood. In this study, we conducted a comparative transcriptome analysis. Rice (WT, art1, star1), barley and rye were exposed to three different solutions (low pH with Al, moderate pH, or high pH with less Fe) for a short period, the roots were subjected to RNA-seq. The difference in gene expression in response to different pH were compared.

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  • イネの有害ミネラル集積を制御する因子の同定

    Grant number：16H02540  2016.04 - 2017.03

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    馬 建鋒, 山地 直樹, 三谷 奈見季

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    Grant amount：\40560000 （ Direct expense: \31200000 、 Indirect expense：\9360000 ）

    これまでにイネのカドミウム集積の品種間差を利用して、幾つかカドミウム集積に関与する新規QTLを検出している。そのうちの一つ、7番染色体に座乗するｑCd7についてファインマッピングを行い、候補遺伝子の絞込みに成功した。この遺伝子の発現をカドミウム集積量の異なる両親間で比較すると、差が認められなかった。またこの遺伝子はカドミウムによる誘導がなかった。両親間の配列を比較すると、1塩基の違いによる１アミノ酸の変化があった。しかし、酵母を用いたアッセイでは、両アリルともカドミウムに対する輸送活性を示した。両親のイネを用いて生理学的な解析も行った。その結果、カドミウム高集積品種では、マンガンによるカドミウムの抑制効果が見られたが、カドミウム低集積品種では、この抑制効果はほとんど見られなかった。これらの結果から、この1アミノ酸の変化はカドミウム輸送に対する親和性が低くなっている可能性があり、現在更なる解析を行っている。
    ヒ素に関しては、ヒ素集積の異なる3種類のイネを用いて、関連遺伝子の発現量を比較した。その結果、ヒ素低集積品種のOsLsi1とOsLsi2の発現量が高集積品種より低いことが分かった。またケイ酸の吸収量も低くなっていた。これらのことはヒ素の低集積がケイ酸輸送体遺伝子の低発現に起因することを示唆している。

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  • Regulation mechanisms of expression for rice Si transporter Lsi1

    Grant number：15H06421  2015.08 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up  Grant-in-Aid for Research Activity Start-up

    MITANI Namiki

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    Grant amount：\3120000 （ Direct expense: \2400000 、 Indirect expense：\720000 ）

    Rice requires high silicon (Si) for its high and sustainable yield. Two Si transporters Lsi1 and Lsi2 are involved in this high accumulation of Si in rice. Our previous studies showed that the mRNA expression levels of these transporter genes were down-regulated by Si. In this project I investigated the mechanism underlying regulation of Lsi1 and Lsi2 expression. There was a negative correlation between the expression level of Lsi1 and Lsi2 and shoot Si accumulation. Together with the results of split-root experiments, the Si-induced down-regulation of Si transporter genes is controlled by shoot Si accumulation, not root Si. Analysis with transgenic rice carrying different lengths of Lsi1 promoter regions fused with GFP as a reporter gene revealed that the region responsible for Si response of Lsi1 expression is present between -327 to -292 in the promoter.

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  • Elucidation of function of rice node for nutrients distribution

    Grant number：15H04469  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Yamaji Naoki, MA Jian Feng

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    Grant amount：\16900000 （ Direct expense: \13000000 、 Indirect expense：\3900000 ）

    Nodes of graminaceous plants containing highly developed and fully organized vascular systems, which involved in “inter-vascular transfer” of mineral nutrients. In this study, we identified novel node localized mineral transporters for phosphorus, boron and iron in rice. Each transporter mediate inter-vascular transfer and contribute to preferential distribution control of each elements. In addition, we conducted promoter analysis of rice silicic acid channel Lsi6, which highly express in xylem transfer cells in enlarged vascular bundles in node. We estimate a regulatory promoter region for the tissue specific gene expression.

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  • Identification and application of genes involved in accumulation of toxic elements in rice

    Grant number：24248014  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    MA Jian Feng, YAMAJI Naoki, MITANI Namiki

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    Grant amount：\44850000 （ Direct expense: \34500000 、 Indirect expense：\10350000 ）

    We identified novel genes involved in accumulation of cadmium (Cd) and arsenic (As) in rice. We found that OsNramp5 is a major transporter for Cd uptake in rice. OsNramp5 is polarly localized at the distal side of root exodermis and endodermis. Over-expression of OsHMA3, a tonoplast-localized Cd transporter, resulted in enhanced tolerance to Cd toxicity. Furthermore, we found that OsHMA2 localized at the node is involved in distribution of Cd to the grain. On the other hand, we found that OsABCC1 reduces As accumulation to the grain in rice. OsABCC1 is mainly localized to the tonoplast of phloem companion cells of the node.

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  • Studies on regulation mechanisms of Si transporters

    Grant number：23780069  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    MITANI Namiki

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    Grant amount：\4550000 （ Direct expense: \3500000 、 Indirect expense：\1050000 ）

    Silicon transporter Lsi1 is involved in high accumulation of Si in rice. Lsi1 is specifically localized at both the exodermis and endodermis in the root and the mRNA expression levels of these transporter genes are down-regulated in response to the accumulation of Si in the shoot. To understand the mechanism regulating the expression and localization of Lsi1, I performed promoter deletion assay. As a result, the candidate region of ciselement for cell-specificity of the Lsi1 localization was located at -1600bp to -1420bp in the upstream. Furthermore, similar analysis with transgenic plants showed that candidate region for the down-regulation of mRNA was located at -600 to -400bp in the upstream of Lsi1.

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  • イネケイ酸吸収遺伝子の機能解析

    Grant number：06J01562  2006 - 2008

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    三谷 奈見季

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    Grant amount：\2800000 （ Direct expense: \2800000 ）

    イネ科植物は一般的にケイ素集積植物とされているが、その集積程度は種によって大きく異なり、イネがオオムギやトウモロコシより高い集積を示す。しかし、これまでにイネやオオムギ、トウモロコシから同様なケイ酸トランスポーター遺伝子が同定されている。本研究ではケイ酸吸収における種間差の機構を明らかにするために。ケイ酸内向きケイ酸トランスポーターLsi1と外向きケイ酸トランスポーターLsi2の局在性、発現量、輸送活性などについてイネ、オオムギとトウモロコシ間で比較検討した。
    オオムギとトウモロコシのLsi1およびLsi2について生化学的手法を用いて細胞内局在性を調べたところ、両者のLsi1,Lsi2とも細胞膜に局在することが明らかになった。抗体染色でLsi2の組織局在性を調べた結果、イネのLsi2とは異なり、オオムギとトウモロコシのLsi2は内皮細胞だけに局在していた。またイネとオオムギとの間で根でのそれぞれの遺伝子の発現量をReal-time RT-PCRを用いて絶対定量法により比較した結果、根におけるmRNAの発現量はLsi1、Lsi2共にオオムギに比ベイネの方が数倍から10倍高かった。さらにアフリカツメガエルoocyteを用いたヘテロ発現系でそれぞれの輸送体の持つケイ酸輸送活性の比較も行った。Lsi2に関してoocyteで発現させた同時にoocyteよりmicrosomeを抽出しwestern blotで発現しているタンパク質の定量を行い、発現タンパク質レベルでの輸送活性を比較した。その結果、イネとオオムギと比較してイネの方がわずかに高かった。これらの結果はイネのケイ酸吸収能力の高さは主にケイ酸吸収関連遺伝子がオオムギやトウモロコシより高いのに加え、トランスポーターの組織局在性に起因することを示唆している。

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  • 【岡山大学】イネの安定多収に欠かせないケイ酸チャネルの構造基盤を解明 Internet

    Racten News (infoseek)  2021.11

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  • 【岡山大学】イネの安定多収に欠かせないケイ酸チャネルの構造基盤を解明 Internet

    産経新聞 (THE SANKEI NEWS)  2021.11

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  • イネの安定多収に欠かせないケイ酸チャネルの構造基盤を解明 岡山大学 Internet

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  • 【岡山大学】イネの安定多収に欠かせないケイ酸チャネルの構造基盤を解明 Internet

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  • 岡山大と東大、イネの安定多収に欠かせないケイ酸チャネルの構造基盤を解明 Internet

    日本経済新聞  2021.10

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