Updated on 2024/02/02

写真a

 
MITANI-Ueno Namiki
 
Organization
Institute of Plant Science and Resources Associate Professor
Position
Associate Professor
External link

Degree

  • 学士(農学) ( 香川大学 )

  • 修士(農学) ( 香川大学 )

  • 博士(農学) ( 岡山大学 )

Research Interests

  • Plant nutrition

  • 植物ストレス

  • 輸送体

  • transporter

  • 植物栄養

  • Plant stress

Research Areas

  • Life Science / Plant nutrition and soil science

Education

  • Okayama University   自然科学研究科バイオサイエンス専攻  

    2006.4 - 2008.9

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Professional Memberships

 

Papers

  • Knockout of a rice K5.2 gene increases Ca accumulation in the grain. Reviewed International journal

    Peitong Wang, Naoki Yamaji, Namiki Mitani-Ueno, Jun Ge, Jian Feng Ma

    Journal of integrative plant biology   2023.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    Rice is a staple food for half world's population, but it is a poor dietary source of calcium (Ca) due to its low concentration. Therefore, it is an important issue to boost the Ca concentration in the grain for improving Ca deficiency risk, but the mechanisms underlying the Ca accumulation are poorly understood. Here, we obtained a rice (Oryza sativa) mutant with high shoot Ca accumulation. The mutant exhibited 26-53% higher Ca in the shoots than that in wild-type rice (WT) at different Ca supplies. The Ca concentration in the xylem sap was 36% higher in the mutant than in the WT. There was no difference in agronomic traits between the WT and mutant, but the mutant showed 25% higher Ca in the polished grain compared with the WT. Map-based cloning combined with complementation test revealed that the mutant phenotype was caused by an 18-bp deletion of a gene, OsK5.2 belonging to Shaker-like K+ channel family. OsK.2 was highly expressed in the mature region of the roots and its expression in the roots was not affected by Ca levels, but up-regulated by low K. Immunostaining showed that OsK.2 was mainly expressed in pericycle of the roots. Taken together, our results revealed a novel role of OsK.2 in Ca translocation in rice, which will be a good target for Ca biofortification in rice. This article is protected by copyright. All rights reserved.

    DOI: 10.1111/jipb.13587

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  • A silicon transporter gene required for healthy growth of rice on land. Reviewed International journal

    Namiki Mitani-Ueno, Naoki Yamaji, Sheng Huang, Yuma Yoshioka, Takaaki Miyaji, Jian Feng Ma

    Nature communications   14 ( 1 )   6522 - 6522   2023.10

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Silicon (Si) is the most abundant mineral element in the earth's crust. Some plants actively accumulate Si as amorphous silica (phytoliths), which can protect plants from stresses. Here, we report a gene (SIET4) that is required for the proper accumulation and cell-specific deposition of Si in rice and show that it is essential for normal growth. SIET4 is constitutively expressed in leaves and encodes a Si transporter. SlET4 polarly localizes at the distal side of epidermal cells and cells surrounding the bulliform cells (motor cells) of the leaf blade, where Si is deposited. Knockout of SIET4 leads to the death of rice in the presence but not absence of Si. Further analysis shows that SIET4 knockout induces abnormal Si deposition in mesophyll cells and the induction of hundreds of genes related to various stress responses. These results indicate that SIET4 is required for the proper export of Si from leaf cells to the leaf surface and for the healthy growth of rice on land.

    DOI: 10.1038/s41467-023-42180-y

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  • Polar localization of a rice silicon transporter requires isoleucine at both C- and N-termini as well as positively charged residues. Reviewed International journal

    Noriyuki Konishi, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    The Plant cell   35 ( 6 )   2232 - 2250   2023.5

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    Silicon (Si) is important for stable and high yields in rice (Oryza sativa), a typical Si hyperaccumulator. The high Si accumulation is achieved by the cooperation of 2 Si transporters, LOW SILICON 1 (OsLsi1) and OsLsi2, which are polarly localized in cells of the root exodermis and endodermis. However, the mechanism underlying their polar localization is unknown. Here, we identified amino acid residues critical for the polar localization of OsLsi1. Deletion of both N- and C-terminal regions resulted in the loss of its polar localization. Furthermore, the deletion of the C-terminus inhibited its trafficking from the endoplasmic reticulum to the plasma membrane. Detailed site-directed mutagenesis analysis showed that Ile18 at the N-terminal region and Ile285 at the C-terminal region were essential for the polar localization of OsLsi1. Moreover, a cluster of positively charged residues at the C-terminal region is also required for polar localization. Phosphorylation and Lys modifications of OsLsi1 are unlikely to be involved in its polar localization. Finally, we showed that the polar localization of OsLsi1 is required for the efficient uptake of Si. Our study not only identified critical residues required for the polar localization of OsLsi1, but also provided experimental evidence for the importance of transporter polarity for efficient nutrient uptake.

    DOI: 10.1093/plcell/koad073

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  • A pericycle‐localized silicon transporter for efficient xylem loading in rice Reviewed

    Sheng Huang, Naoki Yamaji, Gen Sakurai, Namiki Mitani‐Ueno, Noriyuki Konishi, Jian Feng Ma

    New Phytologist   234 ( 1 )   197 - 208   2022.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/nph.17959

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.17959

  • Boron uptake in rice is regulated post-translationally via a clathrin-independent pathway Reviewed

    Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Ji Feng Shao, Namiki Mitani-Ueno, Jian Feng Ma

    Plant Physiology   188 ( 3 )   1649 - 1664   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    Abstract

    Uptake of boron (B) in rice (Oryza sativa) is mediated by the Low silicon rice 1 (OsLsi1) channel, belonging to the NOD26-like intrinsic protein III subgroup, and the efflux transporter B transporter 1 (OsBOR1). However, it is unknown how these transporters cooperate for B uptake and how they are regulated in response to B fluctuations. Here, we examined the response of these two transporters to environmental B changes at the transcriptional and posttranslational level. OsBOR1 showed polar localization at the proximal side of both the exodermis and endodermis of mature root region, forming an efficient uptake system with OsLsi1 polarly localized at the distal side of the same cell layers. Expression of OsBOR1 and OsLsi1 was unaffected by B deficiency and excess. However, although OsLsi1 protein did not respond to high B at the protein level, OsBOR1 was degraded in response to high B within hours, which was accompanied with a significant decrease of total B uptake. The high B-induced degradation of OsBOR1 was inhibited in the presence of MG-132, a proteasome inhibitor, without disturbance of the polar localization. In contrast, neither the high B-induced degradation of OsBOR1 nor its polarity was affected by induced expression of dominant-negative mutated dynamin-related protein 1A (OsDRP1AK47A) or knockout of the mu subunit (AP2M) of adaptor protein-2 complex, suggesting that clathrin-mediated endocytosis is not involved in OsBOR1 degradation and polar localization. These results indicate that, in contrast to Arabidopsis thaliana, rice has a distinct regulatory mechanism for B uptake through clathrin-independent degradation of OsBOR1 in response to high B.

    DOI: 10.1093/plphys/kiab575

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    Other Link: https://academic.oup.com/plphys/article-pdf/188/3/1649/42744142/kiab575.pdf

  • A crucial role for a node-localized transporter, HvSPDT, in loading phosphorus into barley grains. Reviewed International journal

    Mian Gu, Hengliang Huang, Hiroshi Hisano, Guangda Ding, Sheng Huang, Namiki Mitani-Ueno, Kengo Yokosho, Kazuhiro Sato, Naoki Yamaji, Jian Feng Ma

    New phytologist   2022.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    Grains are the major sink of phosphorus (P) in cereal crops, accounting for 60-85% of total plant P, but the mechanisms underlying P loading into the grains are poorly understood. We functionally characterized a transporter gene required for the distribution of P to the grains in barley (Hordeum vulgare), HvSPDT (SULTR-like phosphorus distribution transporter). HvSPDT encoded a plasma membrane-localized Pi/H+ cotransporter. It was mainly expressed in the nodes at both the vegetative and reproductive stages. Furthermore, its expression was induced by inorganic phosphate (Pi) deficiency. In the nodes, HvSPDT was expressed in both the xylem and phloem region of enlarged and diffuse vascular bundles. Knockout of HvSPDT decreased the distribution of P to new leaves, but increased the distribution to old leaves at the vegetative growth stage under low P supply. However, knockout of HvSPDT did not alter the redistribution of P from old to young organs. At the reproductive stage, knockout of HvSPDT significantly decreased P allocation to the grains, resulting in a considerable reduction in grain yield, especially under P-limited conditions. Our results indicate that node-based HvSPDT plays a crucial role in loading P into barley grains through preferentially distributing P from the xylem and further to the phloem.

    DOI: 10.1111/nph.18057

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  • Structural basis for high selectivity of a rice silicon channel Lsi1 Reviewed International journal

    Yasunori Saitoh, Namiki Mitani-Ueno, Keisuke Saito, Kengo Matsuki, Sheng Huang, Lingli Yang, Naoki Yamaji, Hiroshi Ishikita, Jian-Ren Shen, Jian Feng Ma, Michihiro Suga

    Nature Communications   12 ( 1 )   6236 - 6236   2021.12

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>Silicon (Si), the most abundant mineral element in the earth’s crust, is taken up by plant roots in the form of silicic acid through Low silicon rice 1 (Lsi1). Lsi1 belongs to the Nodulin 26-like intrinsic protein subfamily in aquaporin and shows high selectivity for silicic acid. To uncover the structural basis for this high selectivity, here we show the crystal structure of the rice Lsi1 at a resolution of 1.8 Å. The structure reveals transmembrane helical orientations different from other aquaporins, characterized by a unique, widely opened, and hydrophilic selectivity filter (SF) composed of five residues. Our structural, functional, and theoretical investigations provide a solid structural basis for the Si uptake mechanism in plants, which will contribute to secure and sustainable rice production by manipulating Lsi1 selectivity for different metalloids.

    DOI: 10.1038/s41467-021-26535-x

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    Other Link: https://www.nature.com/articles/s41467-021-26535-x

  • A Low Level of NaCl Stimulates Plant Growth by Improving Carbon and Sulfur Assimilation in Arabidopsis thaliana Reviewed

    Li Hongqiao, Akiko Suyama, Namiki Mitani-Ueno, Ruediger Hell, Akiko Maruyama-Nakashita

    Plants   10 ( 10 )   2138 - 2138   2021.10

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    Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    High-salinity stress represses plant growth by inhibiting various metabolic processes. In contrast to the well-studied mechanisms mediating tolerance to high levels of salt, the effects of low levels of salts have not been well studied. In this study, we examined the growth of Arabidopsis thaliana plants under different NaCl concentrations. Interestingly, both shoot and root biomass increased in the presence of 5 mM NaCl, whereas more than 10 mM NaCl decreased plant biomass. To clarify the biological mechanism by which a low level of NaCl stimulated plant growth, we analyzed element accumulation in plants grown under different NaCl concentrations. In addition to the Na and Cl contents, C, S, Zn, and Cu contents were increased under 5 mM NaCl in shoots; this was not observed at higher NaCl concentrations. Adverse effects of high salinity, such as decreased levels of nitrate, phosphate, sulfate, and some cations, did not occur in the presence of 5 mM NaCl. An increase in C was possibly attributed to increased photosynthesis supported by Cl, Zn, and Cu, which also increased in shoots after NaCl application. Salt stress-responsive gene expression was enhanced under 20 mM NaCl but not at lower doses. Among the S metabolites analyzed, cysteine (Cys) was increased by 5 mM NaCl, suggesting that S assimilation was promoted by this dose of NaCl. These results indicate the usefulness of NaCl for plant growth stimulation.

    DOI: 10.3390/plants10102138

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  • Linking transport system of silicon with its accumulation in different plant species Invited Reviewed

    Namiki Mitani-Ueno, Jian Feng Ma

    Soil Science and Plant Nutrition   67 ( 1 )   10 - 17   2021.1

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    Publishing type:Research paper (scientific journal)   Publisher:Informa UK Limited  

    Silicon (Si) is a beneficial element for plants, which helps to mitigate various biotic and abiotic stresses. Since the last review on Si published in this journal in 2004, great progress has been made in understanding transport system of Si in different plant species. The discovery of two different transporters for Si (Lsi1 and Lsi2) in rice led to intensive investigation of Si transporters in other plant species. Lsi1 belongs to the Nodulin 26-like intrinsic proteins (NIPs) subfamily in the aquaporin (AQP) family and functions as an influx transporter for Si. By contrast, Lsi2 belongs to the anion transporter superfamily and functions as an efflux transporter of Si. They are polarly localized at the distal and proximal sides, respectively, of both exodermis and endodermis of rice roots and are required for efficient uptake of Si. So far, homologs of Lsi1 and Lsi2 are identified not only in monocots, but also in dicots, which greatly differ in Si accumulation in the aboveground parts. However, the expression pattern, cell-type-specific expression, and polar localization of these transporters differ with plant species. In this review, we focus on recent progress in Si transporters identified in different plant species. We link these transporters with an accumulation of Si in different plant species in terms of expression pattern, cell-type-specific expression, polar localization of these transporters and propose three uptake systems of Si in different plant species. We also provide the perspectives toward a better understanding of Si transport system in different plant species and discuss its essentiality for plant growth.

    DOI: 10.1080/00380768.2020.1845972

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  • The grapevine NIP2;1 aquaporin is a silicon channel Reviewed

    Henrique Noronha, Angelica Silva, Namiki Mitani-Ueno, Carlos Conde, Farzana Sabir, Catarina Prista, Graca Soveral, Paul Isenring, Jian Feng Ma, Richard R. Belanger, Hernani Geros

    JOURNAL OF EXPERIMENTAL BOTANY   71 ( 21 )   6789 - 6798   2020.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    Silicon (Si) supplementation has been shown to improve plant tolerance to different stresses, and its accumulation in the aerial organs is mediated by NIP2;1 aquaporins (Lsi channels) and Lsi2-type exporters in roots. In the present study, we tested the hypothesis that grapevine expresses a functional NIP2;1 that accounts for root Si uptake and, eventually, Si accumulation in leaves. Own-rooted grapevine cuttings of the cultivar Vinhao accumulated >0.2% Si (DW) in leaves when irrigated with 1.5 mM Si for 1 month, while Si was undetected in control leaves. Real-time PCR showed that VvNIP2;1 was highly expressed in roots and in green berries. The transient transformation of tobacco leaf epidermal cells mediated by Agrobacterium tumefaciens confirmed VvNIP2;1 localization at the plasma membrane. Transport experiments in oocytes showed that VvNIP2;1 mediates Si and arsenite uptake, whereas permeability studies revealed that VvNIP2;1 expressed in yeast is unable to transport water and glycerol. Si supplementation to pigmented grape cultured cells (cv. Gamay Freaux) had no impact on the total phenolic and anthocyanin content, or on the growth rate and VvNIP2;1 expression. Long-term experiments should help determine the extent of Si uptake over time and whether grapevine can benefit from Si fertilization.

    DOI: 10.1093/jxb/eraa294

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  • Node-Localized Transporters of Phosphorus Essential for Seed Development in Rice. Reviewed

    Jing Che, Naoki Yamaji, Takaaki Miyaji, Namiki Mitani-Ueno, Yuri Kato, Ren Fang Shen, Jian Feng Ma

    Plant & cell physiology   61 ( 8 )   1387 - 1398   2020.8

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    About 60-85% of total phosphorus (P) in cereal crops is finally allocated to seeds, where it is required for seed development, germination and early growth. However, little is known about the molecular mechanisms underlying P allocation to seeds. Here, we found that two members (OsPHO1;1 and OsPHO1;2) of the PHO1 gene family are involved in the distribution of P to seeds in rice. Both OsPHO1;1 and OsPHO1;2 were localized to the plasma membrane and showed influx transport activities for inorganic phosphate. At the reproductive stage, both OsPHO1;1 and OsPHO1;2 showed higher expression in node I, the uppermost node connecting to the panicle. OsPHO1;1 was mainly localized at the phloem region of diffuse vascular bundles (DVBs) of node I, while OsPHO1;2 was expressed in the xylem parenchyma cells of the enlarged vascular bundles (EVBs). In addition, they were also expressed in the ovular vascular trace, the outer layer of the inner integument (OsPHO1;1) and in the nucellar epidermis (OsPHO1;2) of caryopses. Knockout of OsPHO1;2, as well as OsPHO1;1 to a lesser extent, decreased the distribution of P to the seed, resulting in decreased seed size and delayed germination. Taken together, OsPHO1;2 expressed in node I is responsible for the unloading of P from the xylem of EVBs, while OsPHO1;1 is involved in reloading P into the phloem of DVBs for subsequent allocation of P to seeds. Furthermore, OsPHO1;1 and OsPHO1;2 expression in the caryopsis is important for delivering P from the maternal tissues to the filial tissues for seed development.

    DOI: 10.1093/pcp/pcaa074

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  • The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions(1)([OPEN]) Reviewed

    Sheng Huang, Akimasa Sasaki, Naoki Yamaji, Haruka Okada, Namiki Mitani-Ueno, Jian Feng Ma

    PLANT PHYSIOLOGY   183 ( 3 )   1224 - 1234   2020.7

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    Zinc (Zn) is an important essential micronutrient for plants and humans; however, the exact transporter responsible for root zinc uptake from soil has not been identified. Here, we found that OsZIP9, a member of the ZRT-IRT-related protein, is involved in Zn uptake in rice (Oryza sativa) under Zn-limited conditions. OsZIP9 was mainly localized to the plasma membrane and showed transport activity for Zn in yeast (Saccharomyces cerevisiae). Expression pattern analysis showed thatOsZIP9was mainly expressed in the roots throughout all growth stages and its expression was upregulated by Zn-deficiency. Furthermore,OsZIP9was expressed in the exodermis and endodermis of root mature regions. For plants grown in a hydroponic solution with low Zn concentration, knockout ofOsZIP9significantly reduced plant growth, which was accompanied by decreased Zn concentrations in both the root and shoot. However, plant growth and Zn accumulation did not differ between knockout lines and wild-type rice under Zn-sufficient conditions. When grown in soil, Zn concentrations in the shoots and grains of knockout lines were decreased to half of wild-type rice, whereas the concentrations of other mineral nutrients were not altered. A short-term kinetic experiment with stable isotope(67)Zn showed that(67)Zn uptake in knockout lines was much lower than that in wild-type rice. Combined, these results indicate that OsZIP9 localized at the root exodermis and endodermis functions as an influx transporter of Zn and contributes to Zn uptake under Zn-limited conditions in rice.The zinc transporter OsZIP9, expressed at the exodermis and endodermis of root mature region, contributes to Zn uptake from soil in rice.

    DOI: 10.1104/pp.20.00125

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  • Tomato roots have a functional silicon influx transporter but not a functional silicon efflux transporter Reviewed

    Hao Sun, Yaoke Duan, Namiki Mitani-Ueno, Jing Che, Jianhua Jia, Jiaqi Liu, Jia Guo, Jian Feng Ma, Haijun Gong

    PLANT CELL AND ENVIRONMENT   43 ( 3 )   732 - 744   2020.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    Silicon (Si) accumulation in shoots differs greatly with plant species, but the molecular mechanisms for this interspecific difference are unknown. Here, we isolated homologous genes of rice Si influx (SlLsi1) and efflux (SlLsi2) transporter genes in tomato (Solanum lycopersicum L.) and functionally characterized these genes. SlLsi1 showed transport activity for Si when expressed in both rice lsi1 mutant and Xenopus laevis oocytes. SlLsi1 was constitutively expressed in the roots. Immunostaining showed that SlLsi1 was localized at the plasma membrane of both root tip and basal region without polarity. Furthermore, overexpression of SlLsi1 in tomato increased Si concentration in the roots and root cell sap but did not alter the Si concentration in the shoots. By contrast, two Lsi2-like proteins did not show efflux transport activity for Si in Xenopus oocytes. However, when functional CsLsi2 from cucumber was expressed in tomato, the Si uptake was significantly increased, resulting in higher Si accumulation in the leaves and enhanced tolerance of the leaves to water deficit and high temperature. Our results suggest that the low Si accumulation in tomato is attributed to the lack of functional Si efflux transporter Lsi2 required for active Si uptake although SlLsi1 is functional.

    DOI: 10.1111/pce.13679

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  • Functional evolution of nodulin 26-like intrinsic proteins: from bacterial arsenic detoxification to plant nutrient transport Reviewed

    Benjamin Pommerrenig, Till A. Diehn, Nadine Bernhardt, Manuela D. Bienert, Namiki Mitani-Ueno, Jacqueline Fuge, Annett Bieber, Christoph Spitzer, Andrea Braeutigam, Jian Feng Ma, Francois Chaumont, Gerd P. Bienert

    NEW PHYTOLOGIST   225 ( 3 )   1383 - 1396   2020.2

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    Nodulin 26-like intrinsic proteins (NIPs) play essential roles in transporting the nutrients silicon and boron in seed plants, but the evolutionary origin of this transport function and the co-permeability to toxic arsenic remains enigmatic. Horizontal gene transfer of a yet uncharacterised bacterial AqpN-aquaporin group was the starting-point for plant NIP evolution. We combined intense sequence, phylogenetic and genetic context analyses and a mutational approach with various transport assays in oocytes and plants to resolve the transorganismal and functional evolution of bacterial and algal and terrestrial plant NIPs and to reveal their molecular transport specificity features. We discovered that aqpN genes are prevalently located in arsenic resistance operons of various prokaryotic phyla. We provided genetic and functional evidence that these proteins contribute to the arsenic detoxification machinery. We identified NIPs with the ancestral bacterial AqpN selectivity filter composition in algae, liverworts, moss, hornworts and ferns and demonstrated that these archetype plant NIPs and their prokaryotic progenitors are almost impermeable to water and silicon but transport arsenic and boron. With a mutational approach, we demonstrated that during evolution, ancestral NIP selectivity shifted to allow subfunctionalisations. Together, our data provided evidence that evolution converted bacterial arsenic efflux channels into essential seed plant nutrient transporters.

    DOI: 10.1111/nph.16217

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  • Vascular Cambium-Localized AtSPDT Mediates Xylem-to-Phloem Transfer of Phosphorus for Its Preferential Distribution in Arabidopsis Reviewed

    Guangda Ding, Gui Jie Lei, Naoki Yamaji, Kengo Yokosho, Namiki Mitani-Ueno, Sheng Huang, Jian Feng Ma

    MOLECULAR PLANT   13 ( 1 )   99 - 111   2020.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:CELL PRESS  

    During plant growth and development mineral elements are preferentially delivered to different organs and tissues to meet the differential demand. It has been shown that the preferential distribution of mineral nutrients in gramineous plants is mediated by node-based transporters, but the mechanisms of preferential distribution in dicots are poorly understood. Here, we report a distinct mechanism for the preferential distribution of phosphorus (P) in Arabidopsis plants, revealed by detailed functional analysis of AtSPDT/AtSULTR3;4 (SULTR-like P Distribution Transporter), a homolog of rice OsSPDT. Like OsSPDT, AtSPDT is localized at the plasma membrane and showed proton-dependent transport activity for P. Interestingly, we found that AtSPDT is mainly expressed in the rosette basal region and leaf petiole, and its expression is up-regulated by P deficiency. Tissue-specific analysis showed that AtSPDT is mainly located in the vascular cambium of different organs, as well as in the parenchyma tissues of both xylem and phloem regions. Knockout of AtSPDT inhibited the growth of new leaves under low P due to decreased P distribution to those organs. The seed yields of the wild-type and atspdt mutant plants are similar, but the seeds of mutant plants contain - less P. These results indicate that AtSPDT localized in the vascular cambium is involved in preferential distribution of P to the developing tissues, through xylem-to-phloem transfer mainly at the rosette basal region and leaf petiole.

    DOI: 10.1016/j.molp.2019.10.002

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  • Transport system of mineral elements in rice Reviewed

    Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    Rice Genomics, Genetics and Breeding   223 - 240   2018.3

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    Language:English   Publishing type:Part of collection (book)   Publisher:Springer Singapore  

    Plant requires 14 mineral elements for their growth and development. These elements in the soil are taken up by the roots, translocated from the roots to the shoots, and distributed to different organs depending on their demands (Marschner P, Mineral nutrition of higher plants, 3rd edn. Academic, London, 2012). In addition to these essential elements, toxic elements such as Cd and As are also transported from the soils to aboveground parts. All these processes require various transporters (membrane proteins). During the last decades, a number of transporters for uptake, translocation, and distribution of mineral elements have been identified, especially in model plants such as Arabidopsis and rice
    however, most transporters remain to be identified. In this chapter, transporters identified so far in rice are described, and the regulation mechanisms of transporters in response to environmental changes are also discussed.

    DOI: 10.1007/978-981-10-7461-5_13

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  • The tonoplast-localized transporter MTP8.2 contributes to manganese detoxification in the shoots and roots of oryza sativa L. Reviewed

    Yuma Takemoto, Yuta Tsunemitsu, Miho Fujii-Kashino, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma, Shin Ichiro Kato, Kozo Iwasaki, Daisei Ueno

    Plant and Cell Physiology   58 ( 9 )   1573 - 1582   2017.9

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    © The Author 2017. Manganese (Mn) cation diffusion facilitators (Mn-CDFs) play important roles in the Mn homeostasis of plants. In rice, the tonoplast-localized Mn-CDF metal tolerance protein 8.1 (MTP8.1) is involved in Mn detoxification in the shoots. This study functionally characterized the Mn-CDF MTP8.2 and determined its contribution to Mn tolerance. MTP8.2 was found to share 68% identity with MTP8.1 and was expressed in both the shoots and roots, but its transcription level was lower than that of MTP8.1. Transient expression of the MTP8.2:green fluorescent protein (GFP) fusion protein and immunoblotting studies indicated that MTP8.2 was also localized to the tonoplast. MTP8.2 expression in yeast conferred tolerance to Mn but not to Fe, Zn, Co, Ni or Cd. MTP8.2 knockdown caused further growth reduction of shoots and roots in the mtp8.1 mutant, which already exhibits stunted growth under conditions of excess Mn. In the presence of high Mn, the MTP8.2 knockdown lines of the mtp8.1 mutant showed lower root Mn concentrations, as well as lower root:total Mn ratios, than those of wild-type rice and the mtp8.1 mutant. These findings indicate that MTP8.2 mediates Mn tolerance along with MTP8.1 through the sequestration of Mn into the shoot and root vacuoles.

    DOI: 10.1093/pcp/pcx082

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  • A model of silicon dynamics in rice: An analysis of the investment efficiency of Si transporters Reviewed

    Gen Sakurai, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Keisuke Ono, Jian Feng Ma

    Frontiers in Plant Science   8   E31-E34   2017.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media S.A.  

    Silicon is the second most abundant element in soils and is beneficial for plant growth. Although, the localizations and polarities of rice Si transporters have been elucidated, the mechanisms that control the expression of Si transporter genes and the functional reasons for controlling expression are not well-understood. We developed a new model that simulates the dynamics of Si in the whole plant in rice by considering Si transport in the roots, distribution at the nodes, and signaling substances controlling transporter gene expression. To investigate the functional reason for the diurnal variation of the expression level, we compared investment efficiencies (the amount of Si accumulated in the upper leaf divided by the total expression level of Si transporter genes) at different model settings. The model reproduced the gradual decrease and diurnal variation of the expression level of the transporter genes observed by previous experimental studies. The results of simulation experiments showed that a considerable reduction in the expression of Si transporter genes during the night increases investment efficiency. Our study suggests that rice has a system that maximizes the investment efficiency of Si uptake.

    DOI: 10.3389/fpls.2017.01187

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  • A Model of Silicon Dynamics in Rice: An Analysis of the Investment Efficiency of Si Transporters Reviewed

    Gen Sakurai, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Keisuke Ono, Jian Feng Ma

    FRONTIERS IN PLANT SCIENCE   8   2017.7

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    Silicon is the second most abundant element in soils and is beneficial for plant growth. Although, the localizations and polarities of rice Si transporters have been elucidated, the mechanisms that control the expression of Si transporter genes and the functional reasons for controlling expression are not well-understood. We developed a new model that simulates the dynamics of Si in the whole plant in rice by considering Si transport in the roots, distribution at the nodes, and signaling substances controlling transporter gene expression. To investigate the functional reason for the diurnal variation of the expression level, we compared investment efficiencies (the amount of Si accumulated in the upper leaf divided by the total expression level of Si transporter genes) at different model settings. The model reproduced the gradual decrease and diurnal variation of the expression level of the transporter genes observed by previous experimental studies. The results of simulation experiments showed that a considerable reduction in the expression of Si transporter genes during the night increases investment efficiency. Our study suggests that rice has a system that maximizes the investment efficiency of Si uptake.

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  • Identification of a mammalian silicon transporter Reviewed

    Sarah Ratcliffe, Ravin Jugdaohsingh, Julien Vivancos, Alan Marron, Rupesh Deshmukh, Jian Feng Ma, Namiki Mitani-Ueno, Jack Robertson, John Wills, Mark V. Boekschoten, Michael Mueller, Robert C. Mawhinney, Stephen D. Kinrade, Paul Isenring, Richard R. Belanger, Jonathan J. Powell

    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY   312 ( 5 )   C550 - C561   2017.5

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    Silicon (Si) has long been known to play a major physiological and structural role in certain organisms, including diatoms, sponges, and many higher plants, leading to the recent identification of multiple proteins responsible for Si transport in a range of algal and plant species. In mammals, despite several convincing studies suggesting that silicon is an important factor in bone development and connective tissue health, there is a critical lack of understanding about the biochemical pathways that enable Si homeostasis. Here we report the identification of a mammalian efflux Si transporter, namely Slc34a2 (also termed NaPiIIb), a known sodium-phosphate cotransporter, which was upregulated in rat kidney following chronic dietary Si deprivation. Normal rat renal epithelium demonstrated punctate expression of Slc34a2, and when the protein was heterologously expressed in Xenopus laevis oocytes, Si efflux activity (i.e., movement of Si out of cells) was induced and was quantitatively similar to that induced by the known plant Si transporter OsLsi2 in the same expression system. Interestingly, Si efflux appeared saturable over time, but it did not vary as a function of extracellular HPO42- or Na+ concentration, suggesting that Slc34a2 harbors a functionally independent transport site for Si operating in the reverse direction to the site for phosphate. Indeed, in rats with dietary Si depletion-induced upregulation of transporter expression, there was increased urinary phosphate excretion. This is the first evidence of an active Si transport protein in mammals and points towards an important role for Si in vertebrates and explains interactions between dietary phosphate and silicon.

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  • Polar Localization of the NIP5;1 Boric Acid Channel Is Maintained by Endocytosis and Facilitates Boron Transport in Arabidopsis Roots Reviewed

    Sheliang Wang, Akira Yoshinari, Tomoo Shimada, Ikuko Hara-Nishimura, Namiki Mitani-Ueno, Jian Feng Ma, Satoshi Naito, Junpei Takano

    PLANT CELL   29 ( 4 )   824 - 842   2017.4

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    Boron uptake in Arabidopsis thaliana is mediated by nodulin 26-like intrinsic protein 5;1 (NIP5;1), a boric acid channel that is located preferentially on the soil side of the plasma membrane in root cells. However, the mechanism underlying this polar localization is poorly understood. Here, we show that the polar localization of NIP5;1 in epidermal and endodermal root cells is mediated by the phosphorylation of Thr residues in the conserved TPG (ThrProGly) repeat in the N-terminal region of NIP5;1. Although substitutions of Ala for three Thr residues in the TPG repeat did not affect lateral diffusion in the plasma membrane, these substitutions inhibited endocytosis and strongly compromised the polar localization of GFP-NIP5;1. Consistent with this, the polar localization was compromised in m subunit mutants of the clathrin adaptor AP2. The Thr-to-Ala substitutions did not affect the boron transport activity of GFP-NIP5;1 in Xenopus laevis oocytes but did inhibit the ability to complement boron translocation to shoots and rescue growth defects in nip5;1-1 mutant plants under boron-limited conditions. These results demonstrate that the polar localization of NIP5;1 is maintained by clathrin-mediated endocytosis, is dependent on phosphorylation in the TPG repeat, and is necessary for the efficient transport of boron in roots.

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  • Reducing phosphorus accumulation in rice grains with an impaired transporter in the node (vol 541, pg 92, 2017) Reviewed

    Naoki Yamaji, Yuma Takemoto, Takaaki Miyaji, Namiki Mitani-Ueno, Kaoru T. Yoshida, Jian Feng Ma

    NATURE   541 ( 7635 )   136 - 136   2017.1

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  • High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice Reviewed

    Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   57 ( 12 )   2510 - 2518   2016.12

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    Rice requires high silicon (Si) for its high and sustainable yield. The efficient uptake of Si in rice is mediated by two transporters OsLsi1 and OsLsi2, which function as influx and efflux transporters, respectively. Our previous studies showed that the mRNA expression levels of these transporter genes were down-regulated by Si. Herein we investigated the mechanism underlying regulation of OsLsi1 and OsLsi2 expression. There was a negative correlation between the expression level of OsLsi1 and OsLsi2 and shoot Si accumulation when the rice seedlings were exposed to different Si supply conditions. A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also downregulated in half the roots without direct Si exposure when the other half of the roots were exposed to Si. Analysis with transgenic rice carrying different lengths of OsLsi1 promoter regions fused with green fluorescent protein (GFP) as a reporter gene revealed that the region responsible for the Si response of OsLsi1 expression is present between -327 to -292 in the promoter. However, this region was not associated with the tissue and cellular localization of OsLsi1. In conclusion, the Si-induced down-regulation of Si transporter genes is controlled by shoot Si, not root Si, and the region between -327 and -292 in the OsLsi1 promoter is involved in this regulation of OsLsi1 expression in rice.

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  • An Aluminum-Inducible IREG Gene is Required for Internal Detoxification of Aluminum in Buckwheat Reviewed

    Kengo Yokosho, Naoki Yamaji, Namiki Mitani-Ueno, Ren Fang Shen, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   57 ( 6 )   1169 - 1178   2016.6

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    Buckwheat (Fagopyrum esculentum Moench) is able to detoxify aluminum (Al) both externally and internally, but the molecular mechanisms underlying its high Al tolerance are not understood. We functionally characterized a gene (FeIREG1) belonging to IRON REGULATED/ferroportin in buckwheat, which showed high expression in our previous genome-wide transcriptome analysis. FeIREG1 was mainly expressed in the roots, and its expression was up-regulated by Al, but not by other metals and low pH. Furthermore, in contrast to AtIREG1 and AtIREG2 in Arabidopsis, the expression of FeIREG1 was not induced by Fe deficiency. Spatial expression analysis showed that the Al-induced expression of FeIREG1 was found in the root tips and higher expression was detected in the outer layers of this part. Immunostaining also showed that FeIREG1 was localized at the outer cell layers in the root tip. A FeIREG1-green fluorescent protein (GFP) fusion protein was localized to the tonoplast when transiently expressed in onion epidermal cells. Overexpression of FeIREG1 in Arabidopsis resulted in increased Al tolerance, but did not alter the tolerance to Cd, Co and Fe. The tolerance to Ni was slightly enhanced in the overexpression lines. Mineral analysis showed that the accumulation of total root Al and other essential mineral elements was hardly altered in the overexpression lines. Taken together, our results suggest that FeIREG1 localized at the tonoplast plays an important role in internal Al detoxification by sequestering Al into the root vacuoles in buckwheat.

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  • A node-localized transporter OsZIP3 is responsible for the preferential distribution of Zn to developing tissues in rice Reviewed

    Akimasa Sasaki, Naoki Yamaji, Namiki Mitani-Ueno, Miho Kashino, Jian Feng Ma

    PLANT JOURNAL   84 ( 2 )   374 - 384   2015.10

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    Developing tissues such as meristem with low transpiration require high Zn levels for their active growth, but the molecular mechanisms underlying the preferential distribution to these tissues are poorly understood. We found that a member of the ZIP (ZRT, IRT-like protein), OsZIP3, showed high expression in the nodes of rice (Oryza sativa). Immunostaining revealed that OsZIP3 was localized at the xylem intervening parenchyma cells and xylem transfer cells of the enlarged vascular bundle in both basal and upper nodes. Neither OsZIP3 gene expression nor encoded protein was affected by either deficiency or toxic levels of Zn. Knockdown of OsZIP3 resulted in significantly reduced Zn levels in the shoot basal region containing the shoot meristem and elongating zone, but increased Zn levels in the transpiration flow. A short-term experiment with the Zn-67 stable isotope showed that more Zn was distributed to the lower leaves, but less to the shoot elongating zone and nodes in the knockdown lines compared with the wild-type rice at both the vegetative and reproductive growth stages. Taken together, OsZIP3 located in the node is responsible for unloading Zn from the xylem of enlarged vascular bundles, which is the first step for preferential distribution of Zn to the developing tissues in rice.
    Significance Statement Zinc homeostasis is achieved by regulating diverse zinc transporters. Here we show that the zinc transporter OsZIP3 mediates the first step leading to preferential distribution of zinc in developing tissues, by unloading zinc from the xylem of enlarged vascular bundles.

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  • Orchestration of three transporters and distinct vascular structures in node for intervascular transfer of silicon in rice Reviewed

    Naoki Yamaji, Gen Sakurai, Namiki Mitani-Ueno, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   112 ( 36 )   11401 - 11406   2015.9

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    Requirement of mineral elements in different plant tissues is not often consistent with their transpiration rate; therefore, plants have developed systems for preferential distribution of mineral elements to the developing tissues with low transpiration. Here we took silicon (Si) as an example and revealed an efficient system for preferential distribution of Si in the node of rice (Oryza sativa). Rice is able to accumulate more than 10% Si of the dry weight in the husk, which is required for protecting the grains from water loss and pathogen infection. However, it has been unknown for a long time how this hyperaccumulation is achieved. We found that three transporters (Lsi2, Lsi3, and Lsi6) located at the node are involved in the intervascular transfer, which is required for the preferential distribution of Si. Lsi2 was polarly localized to the bundle sheath cell layer around the enlarged vascular bundles, which is next to the xylem transfer cell layer where Lsi6 is localized. Lsi3 was located in the parenchyma tissues between enlarged vascular bundles and diffuse vascular bundles. Similar to Lsi6, knockout of Lsi2 and Lsi3 also resulted in decreased distribution of Si to the panicles but increased Si to the flag leaf. Furthermore, we constructed a mathematical model for Si distribution and revealed that in addition to cooperation of three transporters, an apoplastic barrier localized at the bundle sheath cells and development of the enlarged vascular bundles in node are also required for the hyperaccumulation of Si in rice husk.

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  • In Silico Simulation Modeling Reveals the Importance of the Casparian Strip for Efficient Silicon Uptake in Rice Roots Reviewed

    Gen Sakurai, Akiko Satake, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Francois Gabriel Feugier, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   56 ( 4 )   631 - 639   2015.4

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    Silicon (Si) uptake by the roots is mediated by two different transporters, Lsi1 (passive) and Lsi2 (active), in rice (Oryza sativa). Both transporters are polarly localized in the plasma membranes of exodermal (outer) and endodermal (inner) cells with Casparian strips. However, it is unknown how rice is able to take up large amounts of Si compared with other plants, and why rice Si transporters have a characteristic cellular localization pattern. To answer these questions, we simulated Si uptake by rice roots by developing a mathematical model based on a simple diffusion equation that also accounts for active transport by Lsi2. In this model, we calibrated the model parameters using in vivo experimental data on the Si concentrations in the xylem sap and a Monte Carlo method. In our simulation experiments, we compared the Si uptake between roots with various transporter and Casparian strip locations and estimated the Si transport efficiency of roots with different localization patterns and quantities of the Lsi transporters. We found that the Si uptake by roots that lacked Casparian strips was lower than that of normal roots. This suggests that the doublelayer structure of the Casparian strips is an important factor in the high Si uptake by rice. We also found that among various possible localization patterns, the most efficient one was that of the wild-type rice; this may explain the high Si uptake capacity of rice.

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  • A small RNA mediated regulation of a stress-activated retrotransposon and the tissue specific transposition during the reproductive period in Arabidopsis Reviewed

    Wataru Matsunaga, Naohiko Ohama, Noriaki Tanabe, Yukari Masuta, Seiji Masuda, Namiki Mitani, Kazuko Yamaguchi-Shinozaki, Jian F. Ma, Atsushi Kato, Hidetaka Ito

    FRONTIERS IN PLANT SCIENCE   6   48   2015.2

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    Transposable elements (TEs) are key elements that facilitate genome evolution of the host organism. A number of studies have assessed the functions of TEs, which change gene expression in the host genome. Activation of TEs is controlled by epigenetic modifications such as DNA methylation and histone modifications. Several recent studies have reported that TEs can also be activated by biotic or abiotic stress in some plants. We focused on a Ty1/copia retrotransposon, ONSEN, that is activated by heat stress (HS) in Arabidopsis. We found that transcriptional activation of ONSEN was regulated by a small interfering RNA (siRNA)-related pathway, and the activation could also be induced by oxidative stress. Mutants deficient in siRNA biogenesis that were exposed to HS at the initial stages of vegetative growth showed transgenerational transposition. The transposition was also detected in the progeny, which originated from tissue that had differentiated after exposure to the HS. The results indicated that in some undifferentiated cells, transpositional activity could be maintained quite long after exposure to the HS.

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  • Transcriptional activity and silencing of heat activated retrotransposon in Arabidopsis Reviewed

    Wataru Matsunaga, Yukari Masuta, Namiki Mitani, Ma Jian Feng, Atsushi Kato, Hidetaka Ito

    GENES & GENETIC SYSTEMS   89 ( 6 )   316 - 316   2014.12

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  • Physiological and molecular characterization of Si uptake in wild rice species Reviewed

    Namiki Mitani-Ueno, Hisao Ogai, Naoki Yamaji, Jian Feng Ma

    PHYSIOLOGIA PLANTARUM   151 ( 3 )   200 - 207   2014.7

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    Cultivated rice (Oryza sativa) accumulates high concentration of silicon (Si), which is required for its high and sustainable production. High Si accumulation in cultivated rice is achieved by a high expression of both influx (Lsi1) and efflux (Lsi2) Si transporters in roots. Herein, we physiologically investigated Si uptake, isolated and functionally characterized Si transporters in six wild rice species with different genome types. Si uptake by the roots was lower in Oryza rufipogon, Oryza barthii (AA genome), Oryza australiensis (EE genome) and Oryza punctata (BB genome), but similar in Oryza glumaepatula and Oryza meridionalis (AA genome) compared with the cultivated rice (cv. Nipponbare). However, all wild rice species and the cultivated rice showed similar concentration of Si in the shoots when grown in a field. All species with AA genome showed the same amino acid sequence of both Lsi1 and Lsi2 as O. sativa, whereas species with EE and BB genome showed several nucleotide differences in both Lsi1 and Lsi2. However, proteins encoded by these genes also showed transport activity for Si in Xenopus oocyte. The mRNA expression of Lsi1 in all wild rice species was lower than that in the cultivated rice, whereas the expression of Lsi2 was lower in O. rufipogon and O. barthii but similar in other species. Similar cellular localization of Lsi1 and Lsi2 was observed in all wild rice as the cultivated rice. These results indicate that superior Si uptake, the important trait for rice growth, is basically conserved in wild and cultivated rice species.

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  • Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation Reviewed

    Matthew J. Milner, Namiki Mitani-Ueno, Naoki Yamaji, Kengo Yokosho, Eric Craft, Zhangjun Fei, Stephen Ebbs, M. Clemencia Zambrano, Jian Feng Ma, Leon V. Kochian

    PLANT JOURNAL   78 ( 3 )   398 - 410   2014.5

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    The Zn/Cd hyperaccumulator, Noccaea caerulescens, has been studied extensively for its ability to accumulate high levels of Zn and Cd in its leaves. Previous studies have indicated that the Zn and Cd hyperaccumulation trait exhibited by this species involves different transport and tolerance mechanisms. It has also been well documented that certain ecotypes of N.caerulescens are much better Cd hyperaccumulators than others. However, there does not seem to be much ecotypic variation for Zn hyperaccumulation in N.caerulescens. In this study we employed a comparative transcriptomics approach to look at root and shoot gene expression in Ganges and Prayon plants in response to Cd stress to identify transporter genes that were more highly expressed in either the roots or shoots of the superior Cd accumulator, Ganges. Comparison of the transcriptomes from the two ecotypes of Noccaea caerulescens identified a number of genes that encoded metal transporters that were more highly expressed in the Ganges ecotype in response to Cd stress. Characterization of one of these transporters, NcNramp1, showed that it is involved in the influx of Cd across the endodermal plasma membrane and thus may play a key role in Cd flux into the stele and root-to-shoot Cd transport. NcNramp1 may be one of the main transporters involved in Cd hyperaccumulation in N.caerulescens and copy number variation appears to be the main reason for high NcNramp1 gene expression underlying the increased Cd accumulation in the Ganges ecotype.

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  • Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2 Reviewed

    Naoki Yamaji, Jixing Xia, Namiki Mitani-Ueno, Kengo Yokosho, Jian Feng Ma

    PLANT PHYSIOLOGY   162 ( 2 )   927 - 939   2013.6

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    Developing tissues such as meristems and reproductive organs require high zinc, but the molecular mechanisms of how zinc taken up by the roots is preferentially delivered to these tissues with low transpiration are unknown. Here, we report that rice (Oryza sativa) heavy metal ATPase2 (OsHMA2), a member of P-type ATPases, is involved in preferential delivery of zinc to the developing tissues in rice. OsHMA2 was mainly expressed in the mature zone of the roots at the vegetative stage, but higher expression was also found in the nodes at the reproductive stage. The expression was unaffected by either zinc deficiency or zinc excess. OsHMA2 was localized at the pericycle of the roots and at the phloem of enlarged and diffuse vascular bundles in the nodes. Heterologous expression of OsHMA2 in yeast (Saccharomyces cerevisiae) showed influx transport activity for zinc as well as cadmium. Two independent Tos17 insertion lines showed decreased zinc concentration in the crown root tips, decreased concentration of zinc and cadmium in the upper nodes and reproductive organs compared with wild-type rice. Furthermore, a short-term labeling experiment with Zn-67 showed that the distribution of zinc to the panicle and uppermost node I was decreased, but that, to the lower nodes, was increased in the two mutants. Taken together, OsHMA2 in the nodes plays an important role in preferential distribution of zinc as well as cadmium through the phloem to the developing tissues.

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  • Functional Characterization of a Silicon Transporter Gene Implicated in Silicon Distribution in Barley Reviewed

    Naoki Yamaji, Yukako Chiba, Namiki Mitani-Ueno, Jian Feng Ma

    PLANT PHYSIOLOGY   160 ( 3 )   1491 - 1497   2012.11

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    Silicon (Si) is a beneficial element for plant growth. In barley (Hordeum vulgare), Si uptake by the roots is mainly mediated by a Si channel, Low Silicon1 (HvLsi1), and an efflux transporter, HvLsi2. However, transporters involved in the distribution of Si in the shoots have not been identified. Here, we report the functional characterization of a homolog of HvLsi1, HvLsi6. HvLsi6 showed permeability for Si and localized to the plasma membrane. At the vegetative growth stage, HvLsi6 was expressed in both the roots and shoots. The expression level was unaffected by Si supply. In the roots, HvLsi6 was localized in epidermis and cortex cells of the tips, while in the leaf blades and sheaths, HvLsi6 was only localized at parenchyma cells of vascular bundles. At the reproductive growth stage, high expression of HvLsi6 was also found in the nodes. HvLsi6 in node I was polarly located at the transfer cells surrounding the enlarged vascular bundles toward the numerous xylem vessels. These results suggest that HvLsi6 is involved in Si uptake in the root tips, xylem unloading of Si in leaf blade and sheath, and intervascular transfer of Si in the nodes. Furthermore, HvLsi2 was found to be localized at the parenchyma cell layer adjacent to the transfer cells with opposite polarity of HvLsi6, suggesting that the coupling of HvLsi6 and HvLsi2 is involved in the intervascular transfer of Si at the nodes. Si translocated via the enlarged vascular bundles is unloaded to the transfer cells by HvLsi6, followed by HvLsi2 to reload Si to the diffuse vascular bundles, which are connected to the upper part of the plant, especially the panicles, the ultimate Si sink.

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  • Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene Reviewed

    Jonatan Montpetit, Julien Vivancos, Namiki Mitani-Ueno, Naoki Yamaji, Wilfried Remus-Borel, Francois Belzile, Jian Feng Ma, Richard R. Belanger

    PLANT MOLECULAR BIOLOGY   79 ( 1-2 )   35 - 46   2012.5

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    Silicon (Si) is known to be beneficial to plants, namely in alleviating biotic and abiotic stresses. The magnitude of such positive effects is associated with a plant's natural ability to absorb Si. Many grasses can accumulate as much as 10% on a dry weight basis while most dicots, including Arabidopsis, will accumulate less than 0.1%. In this report, we describe the cloning and functional characterization of TaLsi1, a wheat Si transporter gene. In addition, we developed a heterologous system for the study of Si uptake in plants by introducing TaLsi1 and OsLsi1, its ortholog in rice, into Arabidopsis, a species with a very low innate Si uptake capacity. When expressed constitutively under the control of the CaMV 35S promoter, both TaLsi1 and OsLsi1 were expressed in cells of roots and shoots. Such constitutive expression of TaLsi1 or OsLsi1 resulted in a fourfold to fivefold increase in Si accumulation in transformed plants compared to WT. However, this Si absorption caused deleterious symptoms. When the wheat transporter was expressed under the control of a root-specific promoter (a boron transporter gene (AtNIP5;1) promoter), a similar increase in Si absorption was noted but the plants did not exhibit symptoms and grew normally. These results demonstrate that TaLsi1 is indeed a functional Si transporter as its expression in Arabidopsis leads to increased Si uptake, but that this expression must be confined to root cells for healthy plant development. The availability of this heterologous expression system will facilitate further studies into the mechanisms and benefits of Si uptake.

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  • The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic Reviewed

    Namiki Mitani-Ueno, Naoki Yamaji, Fang-Jie Zhao, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   62 ( 12 )   4391 - 4398   2011.8

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    Nodulin-26-like intrinsic proteins (NIPs) of the aquaporin family are involved in the transport of diverse solutes, but the mechanisms controlling the selectivity of transport substrates are poorly understood. The purpose of this study was to investigate how the aromatic/arginine (ar/R) selectivity filter influences the substrate selectivity of two NIP aquaporins; the silicic acid (Si) transporter OsLsi1 (OsNIP2;1) from rice and the boric acid (B) transporter AtNIP5;1 from Arabidopsis; both proteins are also permeable to arsenite. Native and site-directed mutagenized variants of the two genes were expressed in Xenopus oocytes and the transport activities for Si, B, arsenite, and water were assayed. Substitution of the amino acid at the ar/R second helix (H2) position of OsLsi1 did not affect the transport activities for Si, B, and arsenite, but that at the H5 position resulted in a total loss of Si and B transport activities and a partial loss of arsenite transport activity. Conversely, changes of the AtNIP5;1 ar/R selectivity filter and the NPA motifs to the OsLsi1 type did not result in a gain of Si transport activity. B transport activity was partially lost in the H5 mutant but unaffected in the H2 mutant of AtNIP5;1. In contrast, both the single and double mutations at the H2 and/or H5 positions of AtNIP5;1 did not affect arsenite transport activity. The results reveal that the residue at the H5 position of the ar/R filter of both OsLsi1 and AtNIP5;1 plays a key role in the permeability to Si and B, but there is a relatively low selectivity for arsenite.

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  • The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic Reviewed

    Namiki Mitani-Ueno, Naoki Yamaji, Fang-Jie Zhao, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   62 ( 12 )   4391 - 4398   2011.8

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    Nodulin-26-like intrinsic proteins (NIPs) of the aquaporin family are involved in the transport of diverse solutes, but the mechanisms controlling the selectivity of transport substrates are poorly understood. The purpose of this study was to investigate how the aromatic/arginine (ar/R) selectivity filter influences the substrate selectivity of two NIP aquaporins; the silicic acid (Si) transporter OsLsi1 (OsNIP2;1) from rice and the boric acid (B) transporter AtNIP5;1 from Arabidopsis; both proteins are also permeable to arsenite. Native and site-directed mutagenized variants of the two genes were expressed in Xenopus oocytes and the transport activities for Si, B, arsenite, and water were assayed. Substitution of the amino acid at the ar/R second helix (H2) position of OsLsi1 did not affect the transport activities for Si, B, and arsenite, but that at the H5 position resulted in a total loss of Si and B transport activities and a partial loss of arsenite transport activity. Conversely, changes of the AtNIP5;1 ar/R selectivity filter and the NPA motifs to the OsLsi1 type did not result in a gain of Si transport activity. B transport activity was partially lost in the H5 mutant but unaffected in the H2 mutant of AtNIP5;1. In contrast, both the single and double mutations at the H2 and/or H5 positions of AtNIP5;1 did not affect arsenite transport activity. The results reveal that the residue at the H5 position of the ar/R filter of both OsLsi1 and AtNIP5;1 plays a key role in the permeability to Si and B, but there is a relatively low selectivity for arsenite.

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  • Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation. Reviewed

    Mitani N, Yamaji N, Ago Y, Iwasaki K, Ma JF

    The Plant journal : for cell and molecular biology   66 ( 2 )   231 - 240   2011.4

  • Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation Reviewed

    Namiki Mitani, Naoki Yamaji, Yukiko Ago, Kozo Iwasaki, Jian Feng Ma

    PLANT JOURNAL   66 ( 2 )   231 - 240   2011.4

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    P&gt;A high accumulation of silicon (Si) is required for overcoming abiotic and biotic stresses, but the molecular mechanisms of Si uptake, especially in dicotyledonous species, is poorly understood. Herein, we report the identification of an influx transporter of Si in two Cucurbita moschata (pumpkin) cultivars greatly differing in Si accumulation, which are used for the rootstocks of bloom and bloomless Cucumis sativus (cucumber), respectively. Heterogeneous expression in both Xenopus oocytes and rice mutant defective in Si uptake showed that the influx transporter from the bloom pumpkin rootstock can transport Si, whereas that from the bloomless rootstock cannot. Analysis with site-directed mutagenesis showed that, among the two amino acid residues differing between the two types of rootstocks, only changing a proline to a leucine at position 242 results in the loss of Si transport activity. Furthermore, all pumpkin cultivars for bloomless rootstocks tested have this mutation. The transporter is localized in all cells of the roots, and investigation of the subcellular localization with different approaches consistently showed that the influx Si transporter from the bloom pumpkin rootstock was localized at the plasma membrane, whereas the one from the bloomless rootstock was localized at the endoplasmic reticulum. Taken together, our results indicate that the difference in Si uptake between two pumpkin cultivars is probably the result of allelic variation in one amino acid residue of the Si influx transporter, which affects the subcellular localization and subsequent transport of Si from the external solution to the root cells.

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  • Transport of silicon from roots to panicles in plants Reviewed

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani-Ueno

    Proceedings of the Japan Academy Series B: Physical and Biological Sciences   87 ( 7 )   377 - 385   2011

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    Silicon (Si) is the most abundant minerals in soil and exerts beneficial effects on plant growth by alleviating various stresses. The transport of Si from soil to the panicles is mediated by different transporters. Lsi1, belonging to a NIP group of the aquaporin family, is responsible for the uptake of Si from soil into the root cells in both dicots and monocots although its expression patterns and cellular localization differ with plant species. The subsequent transport of Si out of the root cells towards the stele is medicated by an active efflux transporter, Lsi2. Lsi1 and Lsi2 are polarly localized at the distal and proximal sides, respectively, of both exodermis and endodermis in rice root. Silicon in the xylem sap is presented in the form of monosilicic acid and is unloaded by Lsi6, a homolog of Lsi1 in rice. Lsi6 is also involved in the inter-vascular transfer of Si at the node, which is necessary for preferential Si distribution to the panicles. © 2011 The Japan Academy.

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  • Silicon efflux transporters isolated from two pumpkin cultivars contrasting in Si uptake Reviewed

    Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    Plant Signaling and Behavior   6 ( 7 )   73 - 76   2011

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    The accumulation of silicon (Si) differs greatly with plant species and cultivars due to different ability of the roots to take up Si. In Si accumulating plants such as rice, barley and maize, Si uptake is mediated by the influx (Lsi1) and efflux (Lsi2) transporters. Here we report isolation and functional analysis of two Si efflux transporters (CmLsi2-1 and CmLsi2-2) from two pumpkin (Cucurbita moschata Duch.) cultivars contrasting in Si uptake. These cultivars are used for rootstocks of bloom and bloomless cucumber, respectively. Different from mutations in the Si influx transporter CmLsi1, there was no difference in the sequence of either CmLsi2 between two cultivars. Both CmLsi2-1 and CmLsi2-2 showed an efflux transport activity for Si and they were expressed in both the roots and shoots. These results confirm our previous finding that mutation in CmLsi1, but not in CmLsi2-1 and CmLsi2-2 are responsible for bloomless phenotype resulting from low Si uptake. © 2011 Landes Bioscience.

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  • Involvement of Silicon Influx Transporter OsNIP2;1 in Selenite Uptake in Rice Reviewed

    Xue Qiang Zhao, Namiki Mitani, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    PLANT PHYSIOLOGY   153 ( 4 )   1871 - 1877   2010.8

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    Rice (Oryza sativa) as a staple food, provides a major source of dietary selenium (Se) for humans, which essentially requires Se, however, the molecular mechanism for Se uptake is still poorly understood. Herein, we show evidence that the uptake of selenite, a main bioavailable form of Se in paddy soils, is mediated by a silicon (Si) influx transporter Lsi1 (OsNIP2;1) in rice. Defect of OsNIP2;1 resulted in a significant decrease in the Se concentration of the shoots and xylem sap when selenite was given. However, there was no difference in the Se concentration between the wild-type rice and mutant of OsNIP2;1 when selenate was supplied. A short-term uptake experiment showed that selenite uptake greatly increased with decreasing pH in the external solution. Si as silicic acid did not inhibit the Se uptake from selenite in both rice and yeast (Saccharomyces cerevisiae) at low pHs. Expression of OsNIP2;1 in yeast enhanced the selenite uptake at pH 3.5 and 5.5 but not at pH 7.5. On the other hand, defect of Si efflux transporter Lsi2 did not affect the uptake of Se either from selenite or selenate. Taken together, our results indicate that Si influx transporter OsNIP2;1 is permeable to selenite.

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  • The role of the rice aquaporin Lsi1 in arsenite efflux from roots Reviewed

    Fang-Jie Zhao, Yukiko Ago, Namiki Mitani, Ren-Ying Li, Yu-Hong Su, Naoki Yamaji, Steve P. McGrath, Jian Feng Ma

    NEW PHYTOLOGIST   186 ( 2 )   392 - 399   2010

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    When supplied with arsenate (As(V)), plant roots extrude a substantial amount of arsenite (As(III)) to the external medium through as yet unidentified pathways. The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots. Whether Lsi1 also mediates arsenite efflux was investigated.
    Expression of Lsi1 in Xenopus laevis oocytes enhanced arsenite efflux, indicating that Lsi1 facilitates arsenite transport bidirectionally.
    Arsenite was the predominant arsenic species in arsenate-exposed rice plants. During 24-h exposure to 5 mu M arsenate, rice roots extruded arsenite to the external medium rapidly, accounting for 60-90% of the arsenate uptake. A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots. By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium.
    We conclude that Lsi1 plays a role in arsenite efflux in rice roots exposed to arsenate. However, this pathway accounts for only 15-20% of the total efflux, suggesting the existence of other efflux transporters.

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  • The Rice Aquaporin Lsi1 Mediates Uptake of Methylated Arsenic Species Reviewed

    Ren-Ying Li, Yukiko Ago, Wen-Ju Liu, Namiki Mitani, Joerg Feldmann, Steve P. McGrath, Jian Feng Ma, Fang-Jie Zhao

    PLANT PHYSIOLOGY   150 ( 4 )   2071 - 2080   2009.8

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    Pentavalent methylated arsenic (As) species such as monomethylarsonic acid [MMA(V)] and dimethylarsinic acid [DMA(V)] are used as herbicides or pesticides, and can also be synthesized by soil microorganisms or algae through As methylation. The mechanism of MMA(V) and DMA(V) uptake remains unknown. Recent studies have shown that arsenite is taken up by rice (Oryza sativa) roots through two silicon transporters, Lsi1 (the aquaporin NIP2;1) and Lsi2 (an efflux carrier). Here we investigated whether these two transporters also mediate the uptake of MMA(V) and DMA(V). MMA(V) was partly reduced to trivalent MMA(III) in rice roots, but only MMA(V) was translocated to shoots. DMA(V) was stable in plants. The rice lsi1 mutant lost about 80% and 50% of the uptake capacity for MMA(V) and DMA(V), respectively, compared with the wild-type rice, whereas Lsi2 mutation had little effect. The short-term uptake kinetics of MMA(V) can be described by a Michaelis-Menten plus linear model, with the wild type having 3.5-fold higher V(max) than the lsi1 mutant. The uptake kinetics of DMA(V) were linear with the slope being 2.8-fold higher in the wild type than the lsi1 mutant. Heterologous expression of Lsi1 in Xenopus laevis oocytes significantly increased the uptake of MMA(V) but not DMA(V), possibly because of a very limited uptake of the latter. Uptake of MMA(V) and DMA(V) by wild-type rice was increased as the pH of the medium decreased, consistent with an increasing proportion of the undissociated species. The results demonstrate that Lsi1 mediates the uptake of undissociated methylated As in rice roots.

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  • Identification and Characterization of Maize and Barley Lsi2-Like Silicon Efflux Transporters Reveals a Distinct Silicon Uptake System from That in Rice Reviewed

    Namiki Mitani, Yukako Chiba, Naoki Yamaji, Jian Feng Ma

    PLANT CELL   21 ( 7 )   2133 - 2142   2009.7

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    Silicon (Si) uptake has been extensively examined in rice (Oryza sativa), but it is poorly understood in other gramineous crops. We identified Low Silicon Rice 2 (Lsi2)-like Si efflux transporters from two important gramineous crops: maize (Zea mays) and barley (Hordeum vulgare). Both maize and barley Lsi2 expressed in Xenopus laevis oocytes showed Si efflux transport activity. Furthermore, barley Lsi2 was able to recover Si uptake in a rice mutant defective in Si efflux. Maize and barley Lsi2 were only expressed in the roots. Expression of maize and barley Lsi2 was downregulated in response to exogenously applied Si. Moreover, there was a significant positive correlation between the ability of roots to absorb Si and the expression levels of Lsi2 in eight barley cultivars, suggesting that Lsi2 is a key Si transporter in barley. Immunostaining showed that maize and barley Lsi2 localized only at the endodermis, with no polarity. Protein gel blot analysis indicated that maize and barley Lsi2 localized on the plasma membrane. The unique features of maize and barley Si influx and efflux transporters, including their cell-type specificity and the lack of polarity of their localization in Lsi2, indicate that these crops have a different Si uptake system from that in rice.

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  • HvLsi1 is a silicon influx transporter in barley. Reviewed

    Chiba Y, Mitani N, Yamaji N, Ma JF

    The Plant journal : for cell and molecular biology   57 ( 5 )   810 - 818   2009.3

  • A Bacterial-Type ABC Transporter Is Involved in Aluminum Tolerance in Rice Reviewed

    Chao Feng Huang, Naoki Yamaji, Namiki Mitani, Masahiro Yano, Yoshiaki Nagamura, Jian Feng Ma

    PLANT CELL   21 ( 2 )   655 - 667   2009.2

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    Aluminum (Al) toxicity is a major factor limiting crop production in acidic soil, but the molecular mechanisms of Al tolerance are poorly understood. Here, we report that two genes, STAR1 (for sensitive to Al rhizotoxicity1) and STAR2, are responsible for Al tolerance in rice. STAR1 encodes a nucleotide binding domain, while STAR2 encodes a transmembrane domain, of a bacterial-type ATP binding cassette (ABC) transporter. Disruption of either gene resulted in hypersensitivity to aluminum toxicity. Both STAR1 and STAR2 are expressed mainly in the roots and are specifically induced by Al exposure. Expression in onion epidermal cells, rice protoplasts, and yeast showed that STAR1 interacts with STAR2 to form a complex that localizes to the vesicle membranes of all root cells, except for those in the epidermal layer of the mature zone. When expressed together in Xenopus laevis oocytes, STAR1/2 shows efflux transport activity specific for UDP-glucose. Furthermore, addition of exogenous UDP-glucose rescued root growth in the star1 mutant exposed to Al. These results indicate that STAR1 and STAR2 form a complex that functions as an ABC transporter, which is required for detoxification of Al in rice. The ABC transporter transports UDP-glucose, which may be used to modify the cell wall.

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  • OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice Reviewed

    Kengo Yokosho, Naoki Yamaji, Daisei Ueno, Namiki Mitani, Jian Feng Ma

    PLANT PHYSIOLOGY   149 ( 1 )   297 - 305   2009.1

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    Multidrug and toxic compound extrusion (MATE) transporters represent a large family in plants, but their functions are poorly understood. Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1), the closest homolog of barley (Hordeum vulgare) HvAACT1 (aluminum [Al]-activated citrate transporter 1), in terms of metal stress (iron [Fe] deficiency and Al toxicity). This gene was mainly expressed in the roots and the expression level was not affected by either Fe deficiency or Al toxicity. Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root&apos;s stele. The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice. Heterologous expression of OsFRDL1 in Xenopus oocytes showed transport activity for citrate. Immunostaining showed that OsFRDL1 was localized at the pericycle cells of the roots. On the other hand, there was no difference in the Al-induced secretion of citrate from the roots between the knockout line and the wild-type rice. Taken together, our results indicate that OsFRDL1 is a citrate transporter localized at the pericycle cells, which is necessary for efficient translocation of Fe to the shoot as a Fe-citrate complex.

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  • Identification of Maize Silicon Influx Transporters Reviewed

    Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   50 ( 1 )   5 - 12   2009.1

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    Maize (Zea mays L.) shows a high accumulation of silicon (Si), but transporters involved in the uptake and distribution have not been identified. In the present study, we isolated two genes (ZmLsi1 and ZmLsi6), which are homologous to rice influx Si transporter OsLsi1. Heterologous expression in Xenopus laevis oocytes showed that both ZmLsi1 and ZmLsi6 are permeable to silicic acid. ZmLsi1 was mainly expressed in the roots. By contrast, ZmLsi6 was expressed more in the leaf sheaths and blades. Different from OsLsi1, the expression level of both ZmLsi1 and ZmLsi6 was unaffected by Si supply. Immunostaining showed that ZmLsi1 was localized on the plasma membrane of the distal side of root epidermal and hypodermal cells in the seminal and crown roots, and also in cortex cells in lateral roots. In the shoots, ZmLsi6 was found in the xylem parenchyma cells that are adjacent to the vessels in both leaf sheaths and leaf blades. ZmLsi6 in the leaf sheaths and blades also exhibited polar localization on the side facing towards the vessel. Taken together, it can be concluded that ZmLsi1 is an influx transporter of Si, which is responsible for the transport of Si from the external solution to the root cells and that ZmLsi6 mainly functions as a Si transporter for xylem unloading.

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  • NIP1;1, an Aquaporin Homolog, Determines the Arsenite Sensitivity of Arabidopsis thaliana Reviewed

    Takehiro Kamiya, Mayuki Tanaka, Namiki Mitani, Jian Feng Ma, Masayoshi Maeshima, Toru Fujiwara

    JOURNAL OF BIOLOGICAL CHEMISTRY   284 ( 4 )   2114 - 2120   2009.1

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    Arsenite [As(III)] is highly toxic to organisms, including plants. Very recently, transporters in rice responsible for As(III) transport have been described (Ma, J. F., Yamaji, N., Mitani, N., Xu, X. Y., Su, Y. H., McGrath, S. P., and Zhao, F. J. (2008) Proc. Natl. Acad. Sci. U. S. A. 105, 9931-9935), but little is known about As(III) tolerance. In this study, three independent As(III)-tolerant mutants were isolated from ethyl methanesulfonate-mutagenized M2 seeds of Arabidopsis thaliana. All three mutants carried independent mutations in Nodulin 26-like intrinsic protein 1;1 (NIP1;1), a homolog of an aquaporin. Two independent transgenic lines carrying T-DNA in NIP1; 1 were highly tolerant to As(III), establishing that NIP1;1 is the causal gene of As(III) tolerance. Because an aquaglyceroporin is able to transport As(III), we measured As(III) transport activity. When expressed in Xenopus oocytes, NIP1; 1 was capable of transporting As(III). As content in the mutant plants was 30% lower than in wild-type plants. Promoter beta-glucuronidase and real-time PCR analysis showed that NIP1; 1 is highly expressed in roots, and GFP-NIP1;1 is localized to the plasma membrane. These data show that NIP1; 1 is involved in As(III) uptake into roots and that disruption of NIP1; 1 function confers As(III) tolerance to plants. NIP1;2 and NIP5;1, closely related homologs of NIP1;1, were also permeable to As(III). Although the disruption of these genes reduced the As content in plants, As(III) tolerance was not observed in nip1;2 and nip5;1 mutants. This indicates that As(III) tolerance cannot be simply explained by decreased As contents in plants.

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  • Transporters of arsenite in rice and their role in arsenic accumulation in rice grain Reviewed

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Xiao-Yan Xu, Yu-Hong Su, Steve P. McGrath, Fang-Jie Zhao

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   105 ( 29 )   9931 - 9935   2008.7

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    Arsenic poisoning affects millions of people worldwide. Human arsenic intake from rice consumption can be substantial because rice is particularly efficient in assimilating arsenic from paddy soils, although the mechanism has not been elucidated. Here we report that two different types of transporters mediate transport of arsenite, the predominant form of arsenic in paddy soil, from the external medium to the xylem. Transporters belonging to the NIP subfamily of aquaporins in rice are permeable to arsenite but not to arsenate. Mutation in OsNIP2;1 (Lsi1, a silicon influx transporter) significantly decreases arsenite uptake. Furthermore, in the rice mutants defective in the silicon efflux transporter Lsi2, arsenite transport to the xylem and accumulation in shoots and grain decreased greatly. Mutation in Lsi2 had a much greater impact on arsenic accumulation in shoots and grain in field-grown rice than Lsi1. Arsenite transport in rice roots therefore shares the same highly efficient pathway as silicon, which explains why rice is efficient in arsenic accumulation. Our results provide insight into the uptake mechanism of arsenite in rice and strategies for reducing arsenic accumulation in grain for enhanced food safety.

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  • Characterization of substrate specificity of a rice silicon transporter, Lsi1 Reviewed

    Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    Pflugers Archiv European Journal of Physiology   456 ( 4 )   679 - 686   2008.7

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    Lsi1 (OsNIP2
    1) is the first silicon (silicic acid) transporter identified in plant, which belongs to the nodulin 26-like intrinsic membrane protein (NIP) subfamily. In this study, we characterized the function of this transporter by using the Xenopus laevis oocyte expression system. The transport activity of Lsi1 for silicic acid was significantly inhibited by HgCl2 but not by low temperature. Lsi1 also showed an efflux transport activity for silicic acid. The substrate specificity study showed that Lsi1 was able to transport urea and boric acid
    however, the transport activity for silicic acid was not affected by the presence of equimolar urea and was decreased only slightly by boric acid. Furthermore, among the NIPs subgroup, OsNIP2
    2 showed transport activity for silicic acid, whereas OsNIP1
    1 and OsNIP3
    1 did not. We propose that Lsi1 and its close homologues form a unique subgroup of NIP with a distinct ar/R selectivity filter, which is located in the narrowest region on the extra-membrane mouth and govern the substrate specificity of the pore. © 2007 Springer-Verlag.

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  • Transcriptome analysis of the silicon-Magnaporthe grisea interaction

    A. M. Brunings, L. E. Datnoff, J. F. Ma, N. Mitani, Y. Nagamura, B. Rathinasabapathi

    PHYTOPATHOLOGY   98 ( 6 )   S28 - S28   2008.6

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  • Genotypic difference in silicon uptake and expression of silicon transporter genes in rice Reviewed

    Jian Feng Ma, Naoki Yamaji, Kazunori Tamai, Namiki Mitani

    PLANT PHYSIOLOGY   145 ( 3 )   919 - 924   2007.11

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    Rice (Oryza sativa) is a highly silicon (Si)-accumulating species that shows genotypic differences in Si accumulation. We investigated the physiological and molecular mechanisms involved in the genotypic difference in Si uptake between the japonica var. Nipponbare and the indica var. Kasalath. Both the Si concentration in the shoot and the Si uptake per root dry weight were higher in Nipponbare than in Kasalath grown in either soil or nutrient solution. The Si uptake by a single root was also higher in Nipponbare than in Kasalath. A kinetics study showed that Nipponbare and Kasalath had a similar K-m value, whereas the V-max was higher in Nipponbare. The expression of two Si transporter genes (Low silicon rice 1 [Lsi1] and Lsi2) investigated using real-time reverse transcription polymerase chain reaction revealed higher expression of both genes in Nipponbare than in Kasalath. Immunostaining with Lsi1 and Lsi2 antibodies revealed a similar pattern of subcellular localization of these two Si transporters in both varieties; Lsi1 and Lsi2 were localized at the distal and proximal sides, respectively, of both exodermis and endodermis of the roots. These results revealed that the genotypic difference in the Si accumulation results from the difference in abundance of Si transporters in rice roots.

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  • An aluminum-activated citrate transporter in barley. Reviewed

    Jun Furukawa, Naoki Yamaji, Hua Wang, Namiki Mitani, Yoshiko Murata, Kazuhiro Sato, Maki Katsuhara, Kazuyoshi Takeda, Jian Feng Ma

    Plant & cell physiology   48 ( 8 )   1081 - 91   2007.8

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    Soluble ionic aluminum (Al) inhibits root growth and reduces crop production on acid soils. Al-resistant cultivars of barley (Hordeum vulgare L.) detoxify Al by secreting citrate from the roots, but the responsible gene has not been identified yet. Here, we identified a gene (HvAACT1) responsible for the Al-activated citrate secretion by fine mapping combined with microarray analysis, using an Al-resistant cultivar, Murasakimochi, and an Al-sensitive cultivar, Morex. This gene belongs to the multidrug and toxic compound extrusion (MATE) family and was constitutively expressed mainly in the roots of the Al-resistant barley cultivar. Heterologous expression of HvAACT1 in Xenopus oocytes showed efflux activity for (14)C-labeled citrate, but not for malate. Two-electrode voltage clamp analysis also showed transport activity of citrate in the HvAACT1-expressing oocytes in the presence of Al. Overexpression of this gene in tobacco enhanced citrate secretion and Al resistance compared with the wild-type plants. Transiently expressed green fluorescent protein-tagged HvAACT1 was localized at the plasma membrane of the onion epidermal cells, and immunostaining showed that HvAACT1 was localized in the epidermal cells of the barley root tips. A good correlation was found between the expression of HvAACT1 and citrate secretion in 10 barley cultivars differing in Al resistance. Taken together, our results demonstrate that HvAACT1 is an Al-activated citrate transporter responsible for Al resistance in barley.

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  • An efflux transporter of silicon in rice Reviewed

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Kazunori Tamai, Saeko Konishi, Toru Fujiwara, Maki Katsuhara, Masahiro Yano

    NATURE   448 ( 7150 )   209 - U12   2007.7

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    Silicon is an important nutrient for the optimal growth and sustainable production of rice(1-4). Rice accumulates up to 10% silicon in the shoot, and this high accumulation is required to protect the plant from multiple abiotic and biotic stresses(1-5). A gene, Lsi1, that encodes a silicon influx transporter has been identified in rice(6). Here we describe a previously uncharacterized gene, low silicon rice 2 (Lsi2), which has no similarity to Lsi1. This gene is constitutively expressed in the roots. The protein encoded by this gene is localized, like Lsi1, on the plasma membrane of cells in both the exodermis and the endodermis, but in contrast to Lsi1, which is localized on the distal side, Lsi2 is localized on the proximal side of the same cells. Expression of Lsi2 in Xenopus oocytes did not result in influx transport activity for silicon, but preloading of the oocytes with silicon resulted in a release of silicon, indicating that Lsi2 is a silicon efflux transporter. The identification of this silicon transporter revealed a unique mechanism of nutrient transport in plants: having an influx transporter on one side and an efflux transporter on the other side of the cell to permit the effective transcellular transport of the nutrients.

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  • Characterization of silicon permeability of NIP in gramineous plants Reviewed

    Namiki Mitani, Naoki Yamaji, Maki Katsuhara, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   48   S30 - S30   2007

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  • Functional analysis of a rice Si transporter homolog Lsi6. Reviewed

    Naoki Yamaji, Namiki Mitani, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   48   S30 - S30   2007

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  • A silicon transporter in rice. Reviewed

    Ma JF, Tamai K, Yamaji N, Mitani N, Konishi S, Katsuhara M, Ishiguro M, Murata Y, Yano M

    Nature   440 ( 7084 )   688 - 691   2006.3

  • Identification of the silicon form in xylem sap of rice (Oryza sativa L.)

    N Mitani, JF Ma, T Iwashita

    PLANT AND CELL PHYSIOLOGY   46 ( 2 )   279 - 283   2005.2

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    Rice (Oryza sativa L.) is a typical silicon (Si)-accumulating plant, but the mechanism responsible for the translocation from the root to the shoot is poorly understood. In this study, the form of Si in xylem sap was identified by 2 Si-29-nuclear magnetic resonance (NMR) spectroscopy. In rice (cv. Oochikara) cultured in a monosilicic acid solution containing 0.5 mM Si, the Si concentration in the xylem reached 6 mM,l within 30 min. In the Si-29-NMR spectra of the xylem sap, only one signal was observed at a chemical shift of -72.6 ppm, which is consistent with that of monosilicic acid. A H-1-NMR study of xylem sap did not show any significant difference between the wild-type rice and mutant rice defective in Si uptake, and the components of the xylem sap were not affected by the Si supply. The Si concentration in the xylem sap in vitro decreased from an initial 18 mM to 2.6 mM with time. Addition of xylem sap to a solution containing 8 mM Si did not prevent the polymerization of silicic acid. All these results indicate that Si is translocated in the form of monosilicic acid through the xylem and that the concentration of monosilicic acid is high in the xylem only transiently.

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  • Isolation and characterization of Lsi1 Reviewed

    Mitani N, Tamai K, Konishi S, Yano M, Yamaji N, Kyo M, Ma JF

    Plant and Cell Physiology   46   S82   2005

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Books

  • Rice Genomics, Genetics and Breeding

    MITANI-Ueno Namiki( Role: Joint author ,  Transport System of Mineral Elements in Rice.)

    Springer  2018.1 

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MISC

  • Identification of a long-distance signaling protein for regulating Si uptake in rice

    YAMAJI Naoki, MITANI-UENO Namiki, KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   64th   2023

  • 4-1-5 Identification of a gene involved in loading phosphorus into barley grains(4-1 植物の多量栄養素 2022年度東京大会)

    黄 衡亮, 久野 裕, 黄 勝, 三谷 奈見季, 佐藤 和広, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   68   37 - 37   2022.9

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  • P4-1-2 蛍光染色によるイネ葉のケイ素蓄積過程の解析(4-1 植物の多量栄養素 2022年度東京大会)

    山地 直樹, 藤井 理樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   68   41 - 41   2022.9

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  • Identification of the Si transporter Lsi3 involved in efficient xylem loading of Si in rice roots

    HUANG Sheng, YAMAJI Naoki, SAKURAI Gen, MITANI-UENO Namiki, KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   63rd   2022

  • Regulation mechanism of boron uptake in rice

    HUANG Sheng, KONISHI Noriyuki, YAMAJI Naoki, MITANI-UENO Namiki, MA Jian Feng

    日本植物生理学会年会(Web)   63rd   2022

  • 蛍光染色によるイネ葉のケイ素蓄積過程の解析

    山地直樹, 藤井理樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   68   2022

  • Identification of a gene involved in loading phosphorus into barley grains

    黄衡亮, 久野裕, 黄勝, 三谷奈見季, 佐藤和広, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   68   2022

  • 4-2-6 Role of OsLsi6 in B distribution under B-sufficient condition in rice(4-2 植物の微量栄養素 2021年度北海道大会)

    黄 勝, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   60 - 60   2021.9

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  • P4-1-3 イネのケイ素蓄積に関わる輸送体SIET4 の更なる解析(4-1 植物の多量栄養素 2021年度北海道大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   53 - 53   2021.9

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    DOI: 10.20710/dohikouen.67.0_53_2

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  • P4-1-1 ケイ素蓄積に伴うイネ葉身のトランスクリプトーム解析(4-1 植物の多量栄養素 2021年度北海道大会)

    山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   52 - 52   2021.9

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    DOI: 10.20710/dohikouen.67.0_52_3

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  • ケイ素の輸送機構 Reviewed

    三谷奈見季, 馬 建鋒

    日本土壌肥料学雑誌   92 ( 2 )   160 - 165   2021.4

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  • イネのケイ素蓄積に関わる輸送体SIET4の更なる解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021

  • ケイ素蓄積に伴うイネ葉身のトランスクリプトーム解析

    山地直樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021

  • Role of OsLsi6 in B distribution under B-sufficient condition in rice

    黄勝, 山地直樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021

  • Identification of a transporter responsible for Zn uptake in rice

    黄勝, 佐々木明正, 山地直樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   66   2020

  • ケイ酸輸送体Lsi2の相同遺伝子SIET3,4,5の機能解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   66   2020

  • イネのLsi2相同遺伝子の機能解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019

  • イネにおけるケイ素輸送モデルによる輸送体配置の影響分析

    櫻井玄, 山地直樹, 三谷奈見季, 小野圭介, 横沢正幸, 酒井英光, 吉本真由美, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019

  • イネにおける作物体内ミネラル輸送ダイナミクスの解析手法の開発

    櫻井玄, 小野圭介馬建鋒, 山地直樹, 三谷奈見季, 横沢正幸

    農業環境変動研究センター研究成果情報   2018.3

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  • イネ組織間におけるミネラル輸送

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集   63   2017

  • 16 イネのケイ酸吸収に関与する輸送体 Lsi1 と Lsi2 の更なる解析(関西支部講演会,2015年度各支部会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   62 ( 0 )   284 - 284   2016

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    DOI: 10.20710/dohikouen.62.0_284_1

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  • P4-1-6 イネ由来ケイ酸輸送体Lsi1の更なる解析(ポスター,4-1 植物の多量栄養素,2016年度佐賀大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   62 ( 0 )   52 - 52   2016

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    DOI: 10.20710/dohikouen.62.0_52_3

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  • 4-2-4 シロイヌナズナホウ酸チャネルNIP5;1の細胞膜上偏在性は効率的なホウ酸輸送に貢献する(4-2 植物の微量栄養素,2016年度佐賀大会)

    Wang Shelling, 永森 彩奈, 三谷 奈見季, 馬 建鋒, 内藤 哲, 高野 順平

    日本土壌肥料学会講演要旨集   62 ( 0 )   58 - 58   2016

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    DOI: 10.20710/dohikouen.62.0_58_1

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  • コメへの優先的リン分配を担うイネの新規リン酸輸送体

    山地直樹, 竹本侑馬, 宮地孝明, 三谷奈見季, 吉田薫, 馬建鋒

    トランスポーター研究会年会抄録集   11th   2016

  • 膜タンパク質輸送能力の統計的逆推定プロトコルの開発

    櫻井玄, 馬建鋒, 山地直樹, 三谷奈見季

    農研機構農業環境変動研究センター成果情報(Web)   2016   2016

  • 4-1-1 イネ節におけるケイ酸分配機構の統合解析(4-1 植物の多量栄養素,2015年度京都大会)

    山地 直樹, 櫻井 玄, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   61 ( 0 )   48 - 48   2015

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  • 根におけるケイ素吸収・輸送モデルの開発とイネがケイ素を多く吸収できるメカニズムの解明

    櫻井玄, 佐竹暁子, 山地直樹, 横沢正幸, FEUGIER Francois Gabriel, 三谷奈見季, 馬建鋒

    農業環境技術研究所研究成果情報   31   2015

  • 10 イネ野生種におけるケイ酸吸収機構に関する研究(関西支部講演会,2013年度各支部会)

    三谷 奈見季, 大貝 久夫, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   60 ( 0 )   290 - 290   2014

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  • 1-5 イネにおけるケイ素吸収・輸送モデルによる植物体構造とその意義の解明(I 植物栄養と数理モデルの接点-数理モデルで植物栄養の仕組を理解する,シンポジウム,2014年度東京大会)

    櫻井 玄, 佐竹 暁子, 山地 直樹, 三谷 奈見季, 横沢 正幸, 馬 建鋒

    日本土壌肥料学会講演要旨集   60 ( 0 )   191 - 191   2014

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  • 14 イネのマンガン集積に関与する輸送体OsMTP8の解析(関西支部講演会,2013年度各支部会)

    竹本 侑馬, 三谷 奈見季, 佐々木 明正, 山地 直樹, 馬 建鋒, 加藤 伸一郎, 岩崎 貢三, 上野 大勢

    日本土壌肥料学会講演要旨集   60 ( 0 )   291 - 291   2014

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  • シロイヌナズナ熱活性型レトロトランスポゾンの転写活性とサイレンシング

    松永航, 増田ゆかり, 三谷奈見季, 馬建鋒, 加藤敦之, 伊藤秀臣

    日本遺伝学会大会プログラム・予稿集   86th   2014

  • イネのケイ素輸送におけるカスパリー線の役割とトランスポーター配置

    櫻井玄, 馬建鋒, 佐竹暁子, 横沢正幸, 山地直樹, 三谷奈見季

    日本植物生理学会年会要旨集   55th   2014

  • イネの節で局在する輸送体OsZIP3は亜鉛の優先的分配に関与する

    佐々木明正, 山地直樹, 柏野美帆, 三谷奈見季, 馬建鋒

    日本植物生理学会年会要旨集   55th   2014

  • 4-1-2 野生イネ由来ケイ酸トランスポーターの解析(4-1 植物の多量栄養素)

    三谷 奈見季, 大貝 久生, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   59 ( 0 )   54 - 54   2013

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  • イネの節におけるミネラルの維管束間輸送と分配制御機構

    山地直樹, 佐々木明正, 鄭録慶, 夏継星, 横正健剛, 三谷奈見季, 馬建鋒

    トランスポーター研究会年会抄録集   8th   47   2013

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  • イネ由来ケイ酸輸送体Lsi1の発現応答およびその制御機構の解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   54th   2013

  • P9-3 ケイ酸輸送体Lsi1のケイ酸応答性および組織局在性を制御するプロモーターの解析(9.植物の多量栄養素,2012年度鳥取大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   58 ( 0 )   59 - 59   2012

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  • 9-15 オオムギ低親和性硝酸トランスポーターの機能解析(9.植物の多量栄養素)

    石川 伸二, 園部 和幸, 三谷 奈見季, 山地 直樹, 馬 建鋒, 大山 卓爾, 末吉 邦

    日本土壌肥料学会講演要旨集   57 ( 0 )   62 - 62   2011

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  • 11-38 イネ亜ヒ酸排出能の品種間差(11.植物の有害元素)

    大貝 久生, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   57 ( 0 )   91 - 91   2011

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  • 9-28 ケイ酸輸送体Lsi1の発現制御機構の解析(9.植物の多量栄養素)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   57 ( 0 )   67 - 67   2011

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  • 9-3 イネケイ酸輸送体遺伝子Lsi1を過剰発現したシロイヌナズナの解析(9.植物の多量栄養素,2010年度北海道大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   56 ( 0 )   54 - 54   2010

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  • 9-4 イネケイ酸輸送体Lsi3の解析(9.植物の多量栄養素,2010年度北海道大会)

    山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   56 ( 0 )   55 - 55   2010

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  • イネの新規ケイ酸輸送体Lsi3の解析

    山地直樹, 三谷奈見季, 馬建鋒

    日本植物生理学会年会要旨集   51st   2010

  • オオムギのケイ素分配に関与するトランスポーターの解析

    馬建鋒, 山地直樹, 千葉由佳子, 三谷奈見季

    日本植物生理学会年会要旨集   51st   2010

  • オオムギとイネのケイ酸吸収機構の違いに関する研究

    三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   51st   2010

  • 9-20 ブルームレスキュウリ用カボチャ台木のケイ酸吸収特性のさらなる解析(9.植物の多量栄養素,2009年度京都大会)

    馬 建鋒, 吾郷 幸子, 山地 直樹, 三谷 奈見季, 岩崎 貢三

    日本土壌肥料学会講演要旨集   55 ( 0 )   71 - 71   2009

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  • 22 カボチャ及びトマトのケイ酸吸収特性と関連遺伝子の単離(関西支部講演会,2008年度各支部会)

    馬 建鋒, 吾郷 幸子, 山地 直樹, 三谷 奈見季, 岩崎 貢三

    日本土壌肥料学会講演要旨集   55 ( 0 )   298 - 298   2009

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  • Identification of an aquaporin involved in arsenite transport and tolerance.

    Kamiya Takehiro, Tanaka Mayuki, Mitani Namiki, Ma Jian Feng, Maeshima Masayoshi, Fujiwara Toru

    Plant and Cell Physiology Supplement   2009 ( 0 )   S0033 - S0033   2009

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    To identify molecule(s) which mediates As(III) uptake in plants, we screened for As(III) tolerant mutants expecting that loss-of-function mutant of As(III) transporter would be tolerant to As(III). After screening of EMS-mutagenized Arabidopsis thaliana M2 seeds, we identified 3 independent mutants. We also analyzed tolerance of T-DNA mutants of NIPs, which are homologues of aquaglyceroporins. Among the lines tested, nip1;1 mutant showed As(III) tolerance. Sequencing of the NIP1;1 in the mutants revealed that each mutants had an independent mutation in NIP1;1, establishing that NIP1;1 is the causal gene of As(III) tolerance. Xenopus oocytes injected with NIP1;1 cRNA accumulated much As(III) than control oocytes injected with water. The promoter-GUS and real-time PCR analysis showed that NIP1;1 was highly expressed in roots. From these data, we conclude that NIP1;1 is the major As(III) transporter involved in As(III) uptake in roots in A. thaliana.

    DOI: 10.14841/jspp.2009.0.S0033.0

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  • Differential gene expression of rice in response to silicon and rice blast fungus Magnaporthe oryzae

    A. M. Brunings, L. E. Datnoff, J. F. Ma, N. Mitani, Y. Nagamura, B. Rathinasabapathi, M. Kirst

    ANNALS OF APPLIED BIOLOGY   155 ( 2 )   161 - 170   2009

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    Silicon increases the resistance of rice (Oryza sativa) to the rice blast pathogen Magnaporthe oryzae. This study described the relationship between silicon and M. oryzae in terms of whole-genome gene expression. By assessing gene expression patterns in the rice cultivar Monko-to using microarray technology, the physiological basis for silicon-induced resistance was investigated. Silicon amendment resulted in the differential regulation of 221 genes in rice without being challenged with the pathogen. This means that silicon had an observable effect on rice metabolism, as opposed to playing a simple passive role in the resistance response of rice. Compared with control plants, silicon-amended rice differentially regulated 60% less genes, implying that silicon affects the rice response to rice blast infection at a transcriptional level.

    DOI: 10.1111/j.1744-7348.2009.00347.x

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  • オオムギ由来排出型ケイ酸輸送体HvLsi2の更なる機能解析

    三谷奈見季, 山地直樹, 千葉由佳子, 馬建鋒

    日本植物生理学会年会要旨集   50th   2009

  • 9-26 イネのヒ素吸収に関与するトランスポーターの同定(9.植物の無機栄養,2008年度愛知大会)

    馬 建鋒, 山地 直樹, 三谷 奈見季, Zhao Fangjie

    日本土壌肥料学会講演要旨集   54 ( 0 )   78 - 78   2008

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  • P9-30 カボチャ2品種のケイ酸吸収特性の解析(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)

    吾郷 幸子, 三谷 奈見季, 山地 直樹, 岩崎 貢三, 馬 建鋒

    日本土壌肥料学会講演要旨集   54 ( 0 )   95 - 95   2008

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  • P9-31 異なるイネ科植物由来のケイ酸トランスポーター特性の比較解析(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)

    三谷 奈見季, 千葉 由佳子, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   54 ( 0 )   95 - 95   2008

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  • 9-23 植物の金属ストレスにおけるMATE遺伝子の機能解析(9.植物の無機栄養,2008年度愛知大会)

    横正 健剛, 上野 大勢, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   54 ( 0 )   77 - 77   2008

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  • Functional analysis of OsFRDL1, a citrate transporter involved in Fe translocation in rice

    Yokosho Kengo, Ueno Daisei, Yamaji Naoki, Mitani Namiki, Ma Jian Feng

    Plant and Cell Physiology Supplement   2008 ( 0 )   27 - 27   2008

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    We recently identified an Al-tolerant gene (HvAACT1) from barley, which encodes an Al-activated citrate transporter. Here, we report the closest homolog of this gene in rice (OsFRDL1). We compared the Al-induced citrate secretion between the wild-type rice and Tos-17 insertion lines. However, there was no difference in the citrate secretion between these lines. When these lines were grown in a nutrient solution with low Fe concentration, chlorosis on the new leaves was observed only in the knocknot lines. The concentration of Fe and citrate in the xylem sap was much lower and Fe precipitation in the root stele was observed in the knockout line. The protein encoded by this gene showed transport activity for citrate. This gene was expressed in the roots and the expression level was not affected by Fe nutrition. These results suggest that OsFRDL1 is a citrate transporter, which is necessary for Fe translocation to the shoot.

    DOI: 10.14841/jspp.2008.0.0027.0

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  • オオムギにおけるケイ酸トランスポーターの解析

    千葉由佳子, 三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   49th   2008

  • イネのヒ素吸収におけるケイ酸輸送体の関与

    山地直樹, 三谷奈見季, XU Xiao-Yan, MCGRATH Steve P., ZHAO Fang-Jie, 馬建鋒

    日本植物生理学会年会要旨集   49th   2008

  • イネのアルミニウム耐性に関与する細菌タイプABCトランスポーターの解析

    馬建鋒, 山地直樹, 黄朝鋒, 三谷奈見季

    日本植物生理学会年会要旨集   49th   2008

  • トウモロコシのケイ酸トランスポーターの同定

    三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   49th   2008

  • Silicon transporters in rice

    馬 建鋒, 山地 直樹, 三谷 奈見季

    Protein, nucleic acid and enzyme   52 ( 14 )   1849 - 1856   2007.11

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  • P9-40 大麦におけるケイ酸吸収機構の解析(9.植物の無機栄養,2007年度東京大会)

    千葉 由佳子, 三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   89 - 89   2007

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  • 9-41 トウモロコシ由来のケイ酸吸収遺伝子の単離と解析(9.植物の無機栄養,2007年度東京大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   76 - 76   2007

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  • 9-6 オオムギにおけるアルミニウムによるクエン酸分泌に関与する遺伝子の単離と機能解析(9.植物の無機栄養,2007年度東京大会)

    古川 純, 山地 直樹, 王 華, 三谷 奈見季, 村田 佳子, 佐藤 和広, 且原 真木, 武田 和義, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   64 - 64   2007

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  • P9-41 イネのケイ酸輸送体と吸収・集積機構(9.植物の無機栄養,2007年度東京大会)

    山地 直樹, 三谷 奈見季, 玉井 一規, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   90 - 90   2007

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  • イネ科NIPのケイ酸輸送特性の解析

    三谷奈見季, 山地直樹, 且原真木, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007

  • イネケイ酸輸送体Lsi6の機能解析

    山地直樹, 三谷奈見季, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007

  • 9-66 イネケイ酸吸収遺伝子Lsi1の輸送特性の解析(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

    三谷 奈見季, 且原 真木, 馬 建鋒

    日本土壌肥料学会講演要旨集   52 ( 0 )   72 - 72   2006

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  • Functional characterization of a Si transporter gene Lsi1 in rice

    N Mitani, N Yamaji, K Tamai, S Konishi, M Yano, JF Ma

    PLANT AND CELL PHYSIOLOGY   47 ( 0 )   S61 - S61   2006

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    We have cloned a gene (&lt;I&gt;Lsi1&lt;/I&gt;) responsible for Si uptake in rice, a silicon-accumulating plant. In the present study, functional characterization of this gene was conducted. &lt;I&gt;Lsi1&lt;/I&gt; is mainly expressed in the roots. The expression was constitutive, but regulated by Si supply; the expression was decreased by one fourth by continuous Si supply. Lsi1 is expressed in the main roots and lateral roots, but not in root hair. Further investigations show that the transport protein is localized only on the exterior side of plasma membrane of both exodermis and endodermis, where Casparian strips exist. When this gene is suppressed, the Si uptake is also reduced correspondingly. Furthermore, when the cRNA encoding Lsi1 was injected into Xenopus laevis oocytes, an increased transport activity for silicic acid was observed. All these evidence show that Lsi1 is a transporter for silicon in rice roots.

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  • 9-67 イネケイ酸吸収関連遺伝子lsi2の解析(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

    玉井 一規, 山地 直樹, 三谷 奈見季, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   52 ( 0 )   73 - 73   2006

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  • イネのケイ酸輸送体.

    馬 建鋒, 山地直樹, 三谷奈見季, 玉井一規

    細胞工学   25:778-779   2006

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  • 9-26 イネケイ酸吸収関連遺伝子Lsi1の機能解析(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)

    三谷 奈見季, 山地 直樹, 玉井 一規, 小西 左江子, 矢野 昌裕, 村田 佳子, 馬 建鋒

    日本土壌肥料学会講演要旨集   51 ( 0 )   66 - 66   2005

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  • 9-25 新規イネケイ酸吸収関連遺伝子Lsi2の単離と解析(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)

    玉井 一規, 三谷 奈見季, 山地 直樹, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   51 ( 0 )   2005

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  • Uptake system of silicon in different plant species

    MITANI N.

    J. Exp. Bot.   56   1255 - 1261   2005

  • Characterization of the silicon uptake system and molecular mapping of the silicon transporter gene in rice

    JF Ma, N Mitani, S Nagao, S Konishi, K Tamai, T Iwashita, M Yano

    PLANT PHYSIOLOGY   136 ( 2 )   3284 - 3289   2004.10

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    Rice (Oryza sativa L. cv Oochikara) is a typical silicon-accumulating plant, but the mechanism responsible for the high silicon uptake by the roots is poorly understood. We characterized the silicon uptake system in rice roots by using a low-silicon rice mutant (Isi1) and wild-type rice. A kinetic study showed that the concentration of silicon in the root symplastic solution increased with increasing silicon concentrations in the external solution but saturated at a higher concentration in both lines. There were no differences in the silicon concentration of the symplastic solution between the wild-type rice and the mutant. The form of soluble silicon in the root, xylem, and leaf identified by Si-29-NMR was also the same in the two lines. However, the concentration of silicon in the xylem sap was much higher in the wild type than in the mutant. These results indicate that at least two transporters are involved in silicon transport from the external solution to the xylem and that the low-silicon rice mutant is defective in loading silicon into xylem rather than silicon uptake from external solution to cortical cells. To map the responsible gene, we performed a bulked segregant analysis by using both microsatellite and expressed sequence tag-based PCR markers. As a result, the gene was mapped to chromosome 2, flanked by microsatellite marker RM5303 and expressed sequence tag-based PCR marker E60168.

    DOI: 10.1104/pp.104.047365

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  • Characterization of a low Si mutant and molecular mapping of responsible gene in rice

    K Tamai, N Mitani, S Nagao, S Konishi, Y Takeoka, M Yano, JF Ma

    PLANT AND CELL PHYSIOLOGY   45   S49 - S49   2004

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  • 異なる植物におけるケイ酸吸収及び輸送機構に関する解析(9. 植物の無機栄養, 2004年度大会講演要旨集)

    三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   50 ( 0 )   93 - 93   2004

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  • 新規低ケイ酸含量イネ突然変異体 (LSi2) の単離と解析(9. 植物の無機栄養, 2004年度大会講演要旨集)

    武岡 祐子, 三谷 奈見季, 玉井 一規, 佐藤 光, 藤原 徹, 馬 鋒建

    日本土壌肥料学会講演要旨集   50 ( 0 )   92 - 92   2004

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  • イネにおけるケイ酸輸送形態の同定(関西支部講演会, 日本土壌肥料学会支部講演会講演要旨集2004年度)

    三谷 奈見季, 岩下 孝, 馬 建鋒

    日本土壌肥料学会講演要旨集   50 ( 0 )   301 - 301   2004

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  • イネ低ケイ酸含量突然変異体 (lsi1) の解析と原因遺伝子のマッピング(9. 植物の無機栄養, 2004年度大会講演要旨集)

    玉井 一規, 三谷 奈見季, 長尾 咲子, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   50 ( 0 )   93 - 93   2004

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  • 9-68 異なる植物によるケイ酸吸収機構の解析(9.植物の無機栄養)

    三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   49 ( 0 )   87 - 87   2003

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  • 9. 植物の無機栄養 異なる植物によるケイ酸吸収機構の解析

    三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集   49   2003

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Presentations

  • Identification of a gene involved in loading phosphorus into barley grains

    2022.9.13 

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    Event date: 2022.9.13 - 2022.9.15

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  • 蛍光染色によるイネ葉のケイ素蓄積過程の解析

    山地直樹, 藤井理樹, 三谷奈見季, 馬 建鋒

    日本土壌肥料学会2022年度東京大会  2022.9 

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    Event date: 2022.9.13 - 2022.9.15

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  • イネのSIET4輸送体は細胞特異的ケイ素蓄積に関与する

    三谷奈見季, 山地直樹, 吉岡佑真, 宮地孝明, 馬 建鋒

    第63回日本植物生理学会年会(つくば)  2022.3.22 

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    Event date: 2022.3.22 - 2022.3.24

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  • Node-localized transporters of Phosphorus Essential for Seed Development in Rice. Invited

    Che, J, Yamaji, N, Miyaji, T, Mitani-Ueno, N, Kato, Y, Shen, R. F, and Ma, J. F

    2022.3 

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    Event date: 2022.3.22 - 2022.3.24

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  • イネの根における効率的なケイ酸ローディングに寄与する輸送体Lsi3

    黄 勝, 山地直樹, 櫻井 玄, 三谷奈見季, 小西範幸, 馬 建鋒

    第63回日本植物生理学会年会(つくば)  2022.3 

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    Event date: 2022.3.22 - 2022.3.24

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  • Regulation mechanism of boron uptake in rice.

    Huang, S, Konishi, N, Yamaji, N, Mitani-Ueno, N, Ma, J. F

    2022.3 

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    Event date: 2022.3.22 - 2022.3.24

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  • Role of OsLsi6 in B distribution under B-sufficient condition in rice.

    Huang, S, Yamaji N, Mitani N, Ma JF

    2021.9 

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    Event date: 2021.9.14 - 2021.9.16

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  • イネのケイ素蓄積に関わる輸送体SIET4の更なる解析

    三谷奈見季, 山地直樹, 馬 建鋒

    日本土壌肥料学会2021年度北海道大会  2021.9 

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    Event date: 2021.9.14 - 2021.9.16

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  • ケイ素蓄積に伴うイネ葉身のトランスクリプトーム解析

    山地直樹, 三谷奈見季, 馬 建鋒

    日本土壌肥料学会2021年度北海道大会  2021.9 

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    Event date: 2021.9.14 - 2021.9.16

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  • Plasma membrane H+-ATPase is required for active Si uptake in rice.

    Yamamoto, S, Kawai, Y, Okumura, M, You, T, Mitani-Ueno, N, Yamaji, N, Ma, J. F, Kinoshita, T

    2020.3 

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    Event date: 2020.3.19 - 2020.3.21

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  • Identification of a transporter for preferential distribution of phosphorus in Arabidopsis.

    Ding, G, Lei, G. J, Yamaji, N, Yokosho, K, Mitani-Ueno, N, Huang, S, nd Ma, J. F

    2020.3 

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    Event date: 2020.3.19 - 2020.3.21

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  • Integrated micro-scale;macro-scale modeling of silicon transportation system in rice

    Sakurai, G, Yamaji, N, Mitani-Ueno, N, Yokozawa, M, Ono, K, d Ma, J. F

    2019.3 

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    Event date: 2019.3.13 - 2019.3.15

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  • Structure of a silicon transporter in plant. The 60th Annual Meeting of the Japanese Society of Plant Physiologists, Symposium on ‘Plant mineral transporters: from function to structure and modelling’

    Saitoh, Y, Matsuki, K, Yonekura, S, Yang, L, Mitani-Ueno, N, Yamaji, N, Shen, J, Ma, J. F, Suga, M

    2019.3 

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    Event date: 2019.3.13 - 2019.3.15

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  • イネのケイ酸チャネルLsi6の変異体を用いたケイ素吸収・分配制御の詳細解析

    山地直樹, 櫻井 玄, 三谷奈見季, 黄 勝, 馬 建鋒

    日本土壌肥料学会2023年度愛媛大会  2023.9 

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  • Functional characterization of a HvSPDT-like gene in barley

    2023.9 

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  • ケイ素応答性長距離シグナルの同定 Invited

    三谷奈見季, 山地直樹, 小西範幸, 馬 建鋒

    日本土壌肥料学会2023年度愛媛大会  2023.9 

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  • イネのケイ素吸収を制御する長距離シグナルタンパク質の同定

    山地直樹, 三谷奈見季, 小西範幸, 馬 建鋒

    第64回日本植物生理学会年会  2023.3 

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  • Functional characterization of two genes involved in phosphorus loading into barley grains.

    Huang, H, Hisano, H, Huang, S, Mitani-Ueno, N, Sato, K, Yamaji, N, Ma, J. F

    2023.3 

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  • イネのLsi2相同遺伝子の機能解析

    三谷 奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会2019年度静岡大会  2019.9.5 

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  • Identification and functional analysis of transporter genes involved in phosphorus redistribution in rice.

    Mitani-Ueno, N, Yamaji, N, Ma, J. F

    2019.3.15 

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  • A novel phosphate transporter controlling grain P accumulation in rice.

    XVIII International Plant Nutrition Colloquium 2017  2017 

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  • イネ節の新規リン酸輸送体と少燐米の可能性.

    イネ 遺伝学・分子生物学ワークショップ2016  2016 

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  • イネ由来ケイ酸輸送体Lsi1の更なる解析.

    日本土壌肥料学会2016年度大会  2016 

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  • OsSultr3;4 localized in rice node is responsible for preferential distribution of Pi to new leaves and grains.

    The 17th International Workshop on Plant Membrane Biology (IWPMB2016)  2016 

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  • コメへの優先的リン分配を担うイネの新規リン酸輸送体.

    第11回トランスポーター研究会年会,第316回生物圏シンポジウム  2016 

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  • ホウ酸チャネルNIP5;1の細胞膜内偏在はリン酸に依存しホウ酸の効率的な吸収に寄与する.

    第57回日本植物生理学会年会  2016 

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  • イネ節におけるケイ酸分配機構の統合解析.

    日本土壌肥料学会2015年度京都大会  2015 

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  • A co-operated transport system for mineral element uptake in rice roots.

    International Society of Root Research 9th Symposium.  2015 

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  • Phosphorylation-mediated polar localization of a boric acid channel is required for efficient uptake of boron in Arabidopsis thaliana.

    13th International Conference on the Biogeochemistry of Trace Elements.  2015 

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  • Transcriptional regulation of Si transporter genes involved in uptake and distribution in rice.

    6th International Conference on Silicon in Agriculture.  2014 

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  • イネのケイ素輸送におけるカスパリー線の役割とトランスポーター配置.

    第55回日本植物生理学会年会  2014 

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  • Silicon transporters and their role in plants.

    6th International Conference on Silicon in Agriculture.  2014 

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  • イネの節で局在する輸送体OsZIP3は亜鉛の優先的分配に関与する.

    第55回日本植物生理学会年会  2014 

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  • The role of Casparian strips and Lsi transpoter distribution in efficient Si transport in rice.

    6th International Conference on Silicon in Agriculture.  2014 

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  • イネにおけるケイ素吸収・輸送モデルによる植物体構造とその意義の解明.

    日本土壌肥料学会2014年度東京大会  2014 

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  • イネ由来ケイ酸輸送体Lsi1の発現応答およびその制御機構の解析.

    第54回日本植物生理学会年会  2013 

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  • Promoter analysis of a rice influx silicon transporter gene Lsi1.

    16th International Workshop on Plant Membrane Biology.  2013 

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  • イネ節の維管束間輸送に働くケイ酸輸送体.

    第54回日本植物生理学会年会  2013 

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  • イネのマンガン集積に関与する輸送体OsMTP8の解析.

    第109回日本土壌肥料学会関西支部講演会  2013 

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  • イネのマンガンホメオスタシスに関与する輸送体の解析.

    日本土壌肥料学会年会  2013 

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  • イネ野生種におけるケイ酸吸収機構に関する研究.

    第109回日本土壌肥料学会関西支部講演会  2013 

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  • Role of node-located transporters in mineral distribution in rice.

    XVII International Plant Nutrition Colloquium 2013  2013 

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  • 野生イネ由来ケイ酸トランスポーターの解析.

    日本土壌肥料学会年会  2013 

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  • Mineral transport from soil to seed.

    XVII International Plant Nutrition Colloquium 2013  2013 

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  • ケイ酸輸送体Lsi1のケイ酸応答性および組織局在性を制御するプロモーターの解析.

    日本土壌肥料学会年会  2012 

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  • A gene encoding a cysteine-rich peptide is involved in rice Al tolerance.

    The 8th International Symposium on Plant-Soil Interactions at Low pH.  2012 

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  • Preferential Si distribution to the panicles mediated by transporters for inter-vascular transfer at rice node.

    3rd Japan-China Joint Workshop on Plant Nutrition.  2011 

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  • OsCDT3 encoding a cysteine rich peptide is involved in rice Al tolerance.

    International Symposium "Strategies of Plants against Global Environmental Change".  2011 

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  • Transporters involved in preferential distribution of Si to the panicles at the node in rice

    5th International Conference on Silicon in Agriculture  2011 

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  • Isolation and functional analysis of Si transporters in two pumpkin cultivars contrasting in Si uptake.

    5th International Conference on Silicon in Agriculture.  2011 

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  • イネ亜ヒ酸排出能の品種間差.

    日本土壌肥料学会年会  2011 

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  • Transport of silicon from roots to panicles in different plant species

    5th International Conference on Silicon in Agriculture  2011 

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  • ケイ酸輸送体Lsi1の発現制御機構の解析.

    日本土壌肥料学会年会  2011 

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  • ケイ酸トランスポーターLsi1は亜ヒ酸も亜セレン酸も輸送する.

    第3回植物アクアポリン研究検討会  2011 

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  • The biology of silicon.

    The 4th Workshop on the Aqueous Chemistry and Biochemistry of Silicon.  2011 

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  • Isolation and functional characterization of silicon transporters in two pumpkin cultivars contrasting in silicon accumulation.

    3rd Japan-China Joint Workshop on Plant Nutrition.  2011 

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  • イネケイ酸輸送体OsNIP2;1の基質選択性の解析

    第3回植物アクアポリン研究検討会  2011 

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  • Functional analysis of three MATE genes encoding citrate transporter in rice.

    Plant Membrane Biology, 15th International Workshop Adelaide 2011  2010 

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  • イネケイ酸輸送体Lsi3の解析

    日本土壌肥料学会年会  2010 

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  • Transporters involved in inter-vascular transfer of Si at the node of rice.

    Plant Membrane Biology, 15th International Workshop Adelaide 2010  2010 

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  • イネケイ酸輸送体遺伝子Lsi1を過剰発現したシロイヌナズナの解析

    日本土壌肥料学会年会  2010 

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  • イネの新規ケイ酸輸送体Lsi3の解析

    第51回日本植物生理学会年会  2010 

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  • オオムギとイネのケイ酸吸収機構の違いに関する研究

    第51回日本植物生理学会年会  2010 

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Research Projects

  • Molecular basis and substrate selectivity of toxic metalloid channels and transporters

    Grant number:23H02450  2023.04 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    菅 倫寛, 三谷 奈見季

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    Grant amount:\18720000 ( Direct expense: \14400000 、 Indirect expense:\4320000 )

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  • Plasticity of mineral element transport system in response to soil environmental fluctuations in plants

    Grant number:21H05034  2021.07 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

    馬 建鋒, 山地 直樹, 宮地 孝明, 三谷 奈見季, 菅 倫寛

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    Grant amount:\189280000 ( Direct expense: \145600000 、 Indirect expense:\43680000 )

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  • イネ科作物の有害ミネラル集積機構の解明

    Grant number:21H04716  2021.04 - 2025.03

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    馬 建鋒, 山地 直樹, 三谷 奈見季

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    Grant amount:\41860000 ( Direct expense: \32200000 、 Indirect expense:\9660000 )

    1.イネカドミウム集積とヒ素集積 QTL 遺伝子のマッピング
    イネのカドミウム品種間差を利用して作出したマッピング集団を用いて、新しいマーカーを作成しながら、染色体7番と11番のQTL遺伝子の候補領域を狭めることができた。7番染色体にあるQTL遺伝子についてプロモーター領域配列の比較、発現パータンの比較などを行い、この遺伝子の発現の違いがカドミウム集積に寄与している可能性を示した。またカドミウム集積における側根と根毛の役割を解析し、根毛ではなく、側根がカドミウムの吸収に大きな役割を果たしていることを明らかにした。
    ヒ素集積に関して、6番染色体にあるヒ素集積に関与するQTL遺伝子の候補領域を350kbまで狭めることができた。
    2.オオムギのカドミウム集積QTL遺伝子のマッピングとヒ素集積の品種間差
    オオムギのカドミウム集積の品種間差を利用して、4番染色体にあるカドミウム集積に関与するQTL遺伝子のマッピングを行った。いくつかのマーカーを新たに設計し、候補領域を8375kbまで狭めることができた。
    またオオムギヒ素集積の品種間差を調べるために、コアコレクションから100品種を選んで、圃場栽培された種子とわら中のヒ素濃度を測定し、比較した。その結果、ヒ素集積において大きな品種間差があることが分かった。また水耕栽培で幼苗のヒ素集積も比較し、種子のヒ素集積と必ず一致しないことを明らかにした。いくつか集積が大きく異なる品種を選んで、関連遺伝子をマッピングするための交配を行った。

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  • Integrated analysis of mineral ion dynamics in rice crop exposed to acute herbivory stress

    Grant number:21H02196  2021.04 - 2024.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    Ivan Galis, 網干 貴子, 三谷 奈見季

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    Grant amount:\17550000 ( Direct expense: \13500000 、 Indirect expense:\4050000 )

    First year of the project was fully devoted to collection of large scale data for further analyses. Two large scale herbivory experiments have been completed in summer season of FY2021. Two herbivore types (chewing insect larvae and sucking insect adults) were used as proposed after implementing hydroponic rice plant cultivation system. Five tissues (young leaf, attacked leaf, old leaves, stem, and root) over time course of three days have been collected. Out of collected samples, all originally proposed analyses have been carried out; in particular, extensive mineral analysis from 440 rice samples by ICP-MS, phytohormones, and secondary metabolites by LC-MS/MS were performed at IPSR (Galis, Mitani). Sumigraph analysis of total nitrogen and carbon from 440 samples was performed at Yamagata University (Aboshi). Large scale RNA sequencing (RNAseq) of 110 pooled replicated samples was outsourced and analyzed data obtained in March 2022. In outcome, several mineral elements showed differential contents in herbivore attacked plants. Furthermore, large increases in nitrogen containing alkaloids (p-coumaroylputrescine, feruloylputrescine, isopentylamine) were found in the attacked leaves. Jasmonic acid was the main phytohormone elicited in locally attacked leaves. A large number of genes related to defense was elicited in locally attacked leaves but many genes for mineral transport have also been observed in the regulated gene set. Overall, a very large amount of data was obtained that clearly depict extensive changes in rice metabolism in response to herbivore attack.

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  • 低投入を目指したイネ体内におけるミネラル再分配機構の分子生物学的解析

    Grant number:20K05773  2020.04 - 2023.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    三谷 奈見季

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    本年度は、維管束組織で高発現する二つ目の輸送体、SIETについての実験を行った。SIETはケイ酸輸送体Lsi2と比較的相同性の高い輸送体であり、イネにはSIET2-5の4つがある。このうち維管束組織に高発現するのはSIET5である。定量PCRの結果SIET5は生育期間を通して地上部とくに葉鞘で高発現していた。タマネギの表皮細胞やイネプロトプラストを用いた系では細胞内局在がはっきりしなかったことから、抗SIET5抗体を作製し免疫組織染色を行った。その結果SIET5は維管束鞘細胞の細胞膜に局在していることが明らかになった。
    次にゲノム編集技術を用い、変異体の作出を行った。SIETがケイ酸輸送体のホモログであることならびに、主な発現場所が地上部であることを鑑み、地上部のケイ酸の分配に関する表現型の解析を行った。その結果、葉鞘においてケイ素の濃度が野生型より高く、逆に葉身においては低い傾向が見られたが他のミネラルには野生型と遺伝子破壊株の間に差が見られなかった。
    さらにこのSIET5と相同性の高いSIET4の解析も行っている。SIET4はケイ酸の排出輸送活性を持ち、地上部で恒常的に発現していることがあきらかになった。siet4変異体についてはすでに作成済みのため、次年度はその解析を行い、機能を明らかにする。

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  • イネの環境応答統合メカニズムにおける節の役割

    Grant number:19H03250  2019.04 - 2023.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    山地 直樹, 三谷 奈見季, 横正 健剛

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    Grant amount:\17290000 ( Direct expense: \13300000 、 Indirect expense:\3990000 )

    イネ科植物は「節」を構成単位とするモジュール化された体制を採用しており、節には栄養素の分配や成長/休眠の制御などの機能が高度に集約されていることから、個体レベルの環境応答においても様々なシグナルの受発信/変換/転送を担う主要な情報処理の場であると考えられる。
    本年度は、節の環境応答に関与する遺伝子を見出すため、出穂期のイネに対し、短期的なストレス処理を行った最上位節(節I)と下位節(節V~VI)の次世代シーケンサーを用いたRNA-seq解析を実施した。各処理区で発現が有意に変動した転写調節因子、受容体、ペプチドホルモン、などをコードする数十の機能未知遺伝子を選抜した(一次選抜)。さらに定量的RT- PCRによるより詳細な遺伝子発現解析を行い、既存の発現データベース等の情報も加味して検討した結果、節において他の器官よりも特に発現レベルが高く、いずれかのストレス処理に顕著に応答する13の遺伝子を選抜した(二次選抜)。これらの遺伝子についてCRISPR/Cas9法による遺伝子破壊株の作出を進めている。
    イネ節の肥大維管束木部転送細胞に極めて高発現し、ケイ酸の分配を制御する輸送体Lsi6の遺伝子発現を制御する転写調節因子の探索については、酵母one-hybridシステムによるスクリーニングを計画していたが、Lsi6プロモーターの候補領域が酵母において著しい偽陽性を生じることが判明した。
    イネのアルミニウム耐性遺伝子でバクテリア型ABC輸送体のサブユニットをコードするSTAR1については節の維管束系でも高発現していることなどが確認できた。

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  • Study on the regulation mechanism of transformation amenability in barley

    Grant number:19H02930  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    HISANO Hiroshi

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    Grant amount:\17550000 ( Direct expense: \13500000 、 Indirect expense:\4050000 )

    In order to fine map of the three TFA loci involved in the efficiency of genetic transformation of barley, the genetic experiments were conducted using two populations derived from a cross between transformable and non-transformable cultivars. The results revealed the presence of genes contributing to callus induction and differentiation in a region called TFA3. In addition, RNA-seq analysis revealed genes with differential expression between transformable and non-transformable cultivars. One gene with significant expression was cloned and introduced into barley, but no improvement in callus induction efficiency was observed. On the other hand, a genome editing experiment using a TFA-selected barley line succeeded in obtaining individuals with mutations in the target gene.

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  • Identification and functional analysis of transporter genes involved in phosphorus recycling in rice

    Grant number:19K21145  2019.04 - 2020.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up  Grant-in-Aid for Research Activity Start-up

    MITANI-UENO Namiki

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    Grant amount:\2990000 ( Direct expense: \2300000 、 Indirect expense:\690000 )

    Phosphorus (P) able to be redistributed from old to young organs for internal recycling of P, especially under P-limited condition. To identify the transporters that are involved in the P recycling in rice, we performed transcriptomic analysis of vascular bundle tissues from old and young leaves of rice grown under nutrient deficiency condition. Among genes highly expressed in the vascular bundles of old leaves, the protein encoded by OsSultr3;3 showed the P transport activity. This genes localized at the plasma membrane of leaf tissues. Knock out of this genes resulted in P accumulation in old leaves. These results indicated that OsSultr3;3 might be involved in the redistribution of P from source to sink organs.

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  • Integrated analysis of mineral transport system in crops

    Grant number:16H06296  2016.04 - 2021.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Specially Promoted Research  Grant-in-Aid for Specially Promoted Research

    Ma Jian Feng

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    Grant amount:\536250000 ( Direct expense: \412500000 、 Indirect expense:\123750000 )

    We have identified more than 30 transporter genes related to uptake, translocation and distribution of mineral elements including essential, beneficial and toxic elements mainly in rice and buckwheat. We further revealed the mechanisms for response of these transporters to environmental changes, regulation of transporters and their roles in plant growth and productivity by using different approaches. For the first time, we succeeded to crystalize the silicon transporter Lsi1 and revealed its crystal structure. We also constructed a mathematical model for mineral element transport at whole plant scales. These achievements have been published in many international top journals including Nature.

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  • Soil-pH adaptivity trade-off in gramineous plants

    Grant number:16K14871  2016.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    Yamaji Naoki, Ma Jian Feng, Mitani Namiki, Yokosho Kengo

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    Grant amount:\3770000 ( Direct expense: \2900000 、 Indirect expense:\870000 )

    Soil pH has a large impact on availability of mineral elements. For example, at a low pH, Al is solubilized to inhibit plant growth, while at a high pH, Fe availability becomes very low. However, different plant species show different adaptability to soil pH. Among cereal crops, rice is highly tolerant to Al but relatively sensitive to Fe-deficiency. By contrast, barley is the most sensitive to Al but highly tolerant to Fe-deficiency, while rye is able to adapt to a wide range of soil pHs. However, the molecular mechanisms underlying these differences are poorly understood. In this study, we conducted a comparative transcriptome analysis. Rice (WT, art1, star1), barley and rye were exposed to three different solutions (low pH with Al, moderate pH, or high pH with less Fe) for a short period, the roots were subjected to RNA-seq. The difference in gene expression in response to different pH were compared.

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  • イネの有害ミネラル集積を制御する因子の同定

    Grant number:16H02540  2016.04 - 2017.03

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    馬 建鋒, 山地 直樹, 三谷 奈見季

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    Grant amount:\40560000 ( Direct expense: \31200000 、 Indirect expense:\9360000 )

    これまでにイネのカドミウム集積の品種間差を利用して、幾つかカドミウム集積に関与する新規QTLを検出している。そのうちの一つ、7番染色体に座乗するqCd7についてファインマッピングを行い、候補遺伝子の絞込みに成功した。この遺伝子の発現をカドミウム集積量の異なる両親間で比較すると、差が認められなかった。またこの遺伝子はカドミウムによる誘導がなかった。両親間の配列を比較すると、1塩基の違いによる1アミノ酸の変化があった。しかし、酵母を用いたアッセイでは、両アリルともカドミウムに対する輸送活性を示した。両親のイネを用いて生理学的な解析も行った。その結果、カドミウム高集積品種では、マンガンによるカドミウムの抑制効果が見られたが、カドミウム低集積品種では、この抑制効果はほとんど見られなかった。これらの結果から、この1アミノ酸の変化はカドミウム輸送に対する親和性が低くなっている可能性があり、現在更なる解析を行っている。
    ヒ素に関しては、ヒ素集積の異なる3種類のイネを用いて、関連遺伝子の発現量を比較した。その結果、ヒ素低集積品種のOsLsi1とOsLsi2の発現量が高集積品種より低いことが分かった。またケイ酸の吸収量も低くなっていた。これらのことはヒ素の低集積がケイ酸輸送体遺伝子の低発現に起因することを示唆している。

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  • Regulation mechanisms of expression for rice Si transporter Lsi1

    Grant number:15H06421  2015.08 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up  Grant-in-Aid for Research Activity Start-up

    MITANI Namiki

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    Grant amount:\3120000 ( Direct expense: \2400000 、 Indirect expense:\720000 )

    Rice requires high silicon (Si) for its high and sustainable yield. Two Si transporters Lsi1 and Lsi2 are involved in this high accumulation of Si in rice. Our previous studies showed that the mRNA expression levels of these transporter genes were down-regulated by Si. In this project I investigated the mechanism underlying regulation of Lsi1 and Lsi2 expression. There was a negative correlation between the expression level of Lsi1 and Lsi2 and shoot Si accumulation. Together with the results of split-root experiments, the Si-induced down-regulation of Si transporter genes is controlled by shoot Si accumulation, not root Si. Analysis with transgenic rice carrying different lengths of Lsi1 promoter regions fused with GFP as a reporter gene revealed that the region responsible for Si response of Lsi1 expression is present between -327 to -292 in the promoter.

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  • Elucidation of function of rice node for nutrients distribution

    Grant number:15H04469  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Yamaji Naoki, MA Jian Feng

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    Grant amount:\16900000 ( Direct expense: \13000000 、 Indirect expense:\3900000 )

    Nodes of graminaceous plants containing highly developed and fully organized vascular systems, which involved in “inter-vascular transfer” of mineral nutrients. In this study, we identified novel node localized mineral transporters for phosphorus, boron and iron in rice. Each transporter mediate inter-vascular transfer and contribute to preferential distribution control of each elements. In addition, we conducted promoter analysis of rice silicic acid channel Lsi6, which highly express in xylem transfer cells in enlarged vascular bundles in node. We estimate a regulatory promoter region for the tissue specific gene expression.

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  • Identification and application of genes involved in accumulation of toxic elements in rice

    Grant number:24248014  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    MA Jian Feng, YAMAJI Naoki, MITANI Namiki

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    Grant amount:\44850000 ( Direct expense: \34500000 、 Indirect expense:\10350000 )

    We identified novel genes involved in accumulation of cadmium (Cd) and arsenic (As) in rice. We found that OsNramp5 is a major transporter for Cd uptake in rice. OsNramp5 is polarly localized at the distal side of root exodermis and endodermis. Over-expression of OsHMA3, a tonoplast-localized Cd transporter, resulted in enhanced tolerance to Cd toxicity. Furthermore, we found that OsHMA2 localized at the node is involved in distribution of Cd to the grain. On the other hand, we found that OsABCC1 reduces As accumulation to the grain in rice. OsABCC1 is mainly localized to the tonoplast of phloem companion cells of the node.

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  • Studies on regulation mechanisms of Si transporters

    Grant number:23780069  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    MITANI Namiki

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    Silicon transporter Lsi1 is involved in high accumulation of Si in rice. Lsi1 is specifically localized at both the exodermis and endodermis in the root and the mRNA expression levels of these transporter genes are down-regulated in response to the accumulation of Si in the shoot. To understand the mechanism regulating the expression and localization of Lsi1, I performed promoter deletion assay. As a result, the candidate region of ciselement for cell-specificity of the Lsi1 localization was located at -1600bp to -1420bp in the upstream. Furthermore, similar analysis with transgenic plants showed that candidate region for the down-regulation of mRNA was located at -600 to -400bp in the upstream of Lsi1.

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  • イネケイ酸吸収遺伝子の機能解析

    Grant number:06J01562  2006 - 2008

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    三谷 奈見季

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    Grant amount:\2800000 ( Direct expense: \2800000 )

    イネ科植物は一般的にケイ素集積植物とされているが、その集積程度は種によって大きく異なり、イネがオオムギやトウモロコシより高い集積を示す。しかし、これまでにイネやオオムギ、トウモロコシから同様なケイ酸トランスポーター遺伝子が同定されている。本研究ではケイ酸吸収における種間差の機構を明らかにするために。ケイ酸内向きケイ酸トランスポーターLsi1と外向きケイ酸トランスポーターLsi2の局在性、発現量、輸送活性などについてイネ、オオムギとトウモロコシ間で比較検討した。
    オオムギとトウモロコシのLsi1およびLsi2について生化学的手法を用いて細胞内局在性を調べたところ、両者のLsi1,Lsi2とも細胞膜に局在することが明らかになった。抗体染色でLsi2の組織局在性を調べた結果、イネのLsi2とは異なり、オオムギとトウモロコシのLsi2は内皮細胞だけに局在していた。またイネとオオムギとの間で根でのそれぞれの遺伝子の発現量をReal-time RT-PCRを用いて絶対定量法により比較した結果、根におけるmRNAの発現量はLsi1、Lsi2共にオオムギに比ベイネの方が数倍から10倍高かった。さらにアフリカツメガエルoocyteを用いたヘテロ発現系でそれぞれの輸送体の持つケイ酸輸送活性の比較も行った。Lsi2に関してoocyteで発現させた同時にoocyteよりmicrosomeを抽出しwestern blotで発現しているタンパク質の定量を行い、発現タンパク質レベルでの輸送活性を比較した。その結果、イネとオオムギと比較してイネの方がわずかに高かった。これらの結果はイネのケイ酸吸収能力の高さは主にケイ酸吸収関連遺伝子がオオムギやトウモロコシより高いのに加え、トランスポーターの組織局在性に起因することを示唆している。

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Media Coverage

  • 【岡山大学】イネの安定多収に欠かせないケイ酸チャネルの構造基盤を解明 Internet

    産経新聞 (THE SANKEI NEWS)  2021.11

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  • イネの安定多収に欠かせないケイ酸チャネルの構造基盤を解明 岡山大学 Internet

    JA Com (農業協同組合新聞)  2021.11

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  • 【岡山大学】イネの安定多収に欠かせないケイ酸チャネルの構造基盤を解明 Internet

    シブヤ経済新聞  2021.11

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  • 【岡山大学】イネの安定多収に欠かせないケイ酸チャネルの構造基盤を解明 Internet

    Racten News (infoseek)  2021.11

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  • 岡山大と東大、イネの安定多収に欠かせないケイ酸チャネルの構造基盤を解明 Internet

    日本経済新聞  2021.10

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