研究者詳細 - 荒川　健佑

論文 - 荒川　健佑

分割表示 56 件中 21 - 40 件目／全件表示 >>

Draft genome sequence of Bifidobacterium lemurum DSM 28807T isolated from the gastrointestinal tracts of ring-tailed lemur (Lemur catta). 査読

Hidehiro Toh, Takehiro Matsubara, Shuta Tomida, Iyo Mimura, Kensuke Arakawa, Takefumi Kikusui, Hidetoshi Morita

Genome Announcements 5 e01656-16 2016年

上部消化管内優勢乳酸桿菌種のペプチド要求性に関する研究. 招待

荒川健佑

IFO Research Communications 30 127 - 128 2016年

Draft Genome Sequence of Bifidobacterium aesculapii DSM 26737 T , Isolated from Feces of Baby Common Marmoset 査読

Hidehiro Toh, Yumiko Yamazaki, Kosuke Tashiro, Shinpei Kawarai, Kenshiro Oshima, Akiyo Nakano, Co Nguyen Thi Kim, Iyo Mimura, Kensuke Arakawa, Atsushi Iriki, Takefumi Kikusui, Hidetoshi Morita

Genome Announcements 3 ( 6 ) e01463-15 2015年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：American Society for Microbiology

<italic>Bifidobacterium aesculapii</italic>
DSM 26737
^T
was isolated from feces of baby common marmoset. Here, we report the draft genome sequence of this organism. This paper is the first published report of the genomic sequence of
<italic>B. aesculapii</italic>
.

DOI： 10.1128/genomea.01463-15

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Complete genome sequence of Bifidobacterium angulatum JCM 7096(T) isolated from human feces 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Misa Kiuchi, Hiromi Kuroyanagi, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

JOURNAL OF BIOTECHNOLOGY 211 10 - 11 2015年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Bifidobacterium angulatum JCM 7096T was isolated from human feces. Here we report the complete genome sequence of this organism. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. angulatum strain. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.jbiotec.2015.06.412

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Complete genome sequence of Bifidobacterium catenulatum JCM 1194(T) isolated from human feces 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Naoko Yamashita, Erica Iioka, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

JOURNAL OF BIOTECHNOLOGY 210 25 - 26 2015年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Bifidobacterium catenulatum JCM 1194(T) was isolated from human feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. catenulatum strain. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.jbiotec.2015.06.415

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Complete genome sequence of Bifidobacterium breve JCM 1192(T) isolated from infant feces 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Emi Omori, Yasue Hattori, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

JOURNAL OF BIOTECHNOLOGY 210 81 - 82 2015年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

DOI： 10.1016/j.jbiotec.2015.06.414

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Complete genome sequence of Bifidobacterium bifidum JCM 1255(T) isolated from feces of a breast-fed infant 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Chie Shindo, Keiko Komiya, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

JOURNAL OF BIOTECHNOLOGY 210 66 - 67 2015年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

DOI： 10.1016/j.jbiotec.2015.06.413

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Complete genome sequence of Bifidobacterium pseudocatenulatum JCM 1200(T) isolated from infant feces 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Kensuke Arakawa, Yukiko Takayama, Rina Kurokawa, Kageyasu Takanashi, Kenya Honda, Masahira Hattori

JOURNAL OF BIOTECHNOLOGY 210 68 - 69 2015年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Bifidobacterium pseudocatenulatum JCM 1200(T) was isolated from infant feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. pseudocatenulatum strain. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.jbiotec.2015.06.416

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Lactobacillus gasseri requires peptides, not proteins or free amino acids, for growth in milk 査読

K. Arakawa, K. Matsunaga, S. Takihiro, A. Moritoki, S. Ryuto, Y. Kawai, T. Masuda, T. Miyamoto

JOURNAL OF DAIRY SCIENCE 98 ( 3 ) 1593 - 1603 2015年3月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE INC

Lactobacillus gasseri is a widespread commensal lactic acid bacterium inhabiting human mucosal niches and has many beneficial effects as a probiotic. However, L. gasseri is difficult to grow in milk, which hurts usability for the food industry. It had been previously reported that supplementation with yeast extract or proteose peptone, including peptides, enables L. gasseri to grow well in milk. In this study, our objective was to confirm peptide requirement of L. gasseri and evaluate efficacy of peptide release by enzymatic proteolysis on growth of L. gassei in milk. Three strains of L. gasseri did not grow well in modified DeMan, Rogosa, Sharpe broth without any nitrogen sources (MRS-N), but addition of a casein-derived peptide mixture, tryptone, promoted growth. In contrast, little effect was observed after adding casein or a casein-derived amino acid mixture, casamino acids. These results indicate that L. gasseri requires peptides, not proteins or free amino acids, among milk-derived nitrogen sources for growth. Lactobacillus gasseri JCM 1131(T) hardly had growth capacity in 6 kinds of milk-based media: bovine milk, human milk, skim milk, cheese whey, modified MRS-N (MRSL-N) supplemented with acid whey, and MRSL-N supplemented with casein. Moreover, treatment with digestive proteases, particularly pepsin, to release peptides made it grow well in each milk-based medium. The pepsin treatment was the most effective for growth of strain JCM 1131(T) in skim milk among the tested food-grade proteases such as trypsin, alpha-chymotrypsin, calf rennet, ficin, bromelain, and papain. As well as strain JCM 1131(T), pepsinolysis of milk improved growth of other L. gasseri strains and some strains of enteric lactobacilli such as Lactobacillus crispatus, Lactobacillus gallinarum, Lactobacillus johnsonii, and Lactobacillus reuteri. These results suggest that some relatives of L. gasseri also use peptides as desirable nitrogen sources, and that milk may be a good supplier of nutritious peptides to enteric lactobacilli including L. gasseri after peptic digestion in the gastrointestinal tract. This is the first report showing peptide requirement of L. gasseri and efficacy of pepsinolysis on the growth of L. gasseri and its relatives in milk. This study would contribute to increasing usability of L. gasseri and its relatives as probiotics in dairy foods.

DOI： 10.3168/jds.2014-8860

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発酵豆乳に含まれる大豆たん白質由来抗菌ペプチドの特性解析. 招待

荒川健佑, 福留香菜子, 豊川直樹, 大森葉子, 宮本拓

大豆たん白質研究 18 204 - 212 2015年

乳酸菌あれこれ #62 多様性の話. 招待

荒川健佑

日本乳酸菌学会誌 25 55 - 55 2014年

Genetic and biochemical evidence for gassericin T production from Lactobacillus gasseri LA158. 査読

Narimi Yasuta, Kensuke Arakawa, Yasushi Kawai, Takahiro Chujo, Kiyoshi Nakamura, Haruka Suzuki, Yoshiyuki Ito, Junko Nishimura, Yumiko Makino, Shuji Shigenobu, Tadao Saito

Milk Science 63 9 - 17 2014年

Food preservative potential of gassericin A-containing concentrate prepared from cheese whey culture supernatant of Lactobacillus gasseri LA39 査読

Kiyoshi Nakamura, Kensuke Arakawa, Yasushi Kawai, Narimi Yasuta, Takahiro Chujo, Masamichi Watanabe, Hiroyuki Iioka, Masashi Tanioka, Junko Nishimura, Haruki Kitazawa, Koichi Tsurumi, Tadao Saito

Animal Science Journal 84 ( 2 ) 144 - 149 2013年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Gassericin A (GA) is a circular bacteriocin produced by Lactobacillus gasseri LA39. In this study, GA-containing concentrate was prepared using a cross-flow membrane filtration device (30kDa cut-off) from the culture supernatant of Lb.gasseri LA39 cultivated in a cheese whey-based food-grade medium. The bacteriocin activity titer in the concentrate was 16 times as high as that of the culture supernatant and was completely maintained through each incubation at 4°C for 3months, 37°C for 2months, 60°C for 5 h, and 100°C for 30min. The GA-containing concentrate was used with glycine powder to make custard creams, and then four representative strains of custard cream spoilage bacteria (Bacillus cereus, Lactococcus lactis subsp. lactis, Achromobacter denitrificans and Pseudomonas fluorescens) were individually inoculated at c. 103 colony forming units/g in the custard creams. Throughout 30days of incubation at 30°C, all of the inoculated bacteria were completely inhibited by the combination of 5% (w/w) of the GA-containing concentrate and 0.5% (w/w) glycine. This is the first highly practical application of GA to foods as a biopreservative, and the concentration method and the bacteriocin concentrate would contribute to biopreservation of several foods. © 2012 Japanese Society of Animal Science.

DOI： 10.1111/j.1740-0929.2012.01048.x

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Interaction between lactic acid bacteria and yeasts in airag, an alcoholic fermented milk 査読

Sudun, Wulijideligen, Kensuke Arakawa, Mari Miyamoto, Taku Miyamoto

Animal Science Journal 84 ( 1 ) 66 - 74 2013年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

The interaction between nine lactic acid bacteria (LAB) and five yeast strains isolated from airag of Inner Mongolia Autonomic Region, China was investigated. Three representative LAB and two yeasts showed symbioses were selected and incubated in 10% (w/v) reconstituted skim milk as single and mixed cultures to measure viable count, titratable acidity, ethanol and sugar content every 24h for 1week. LAB and yeasts showed high viable counts in the mixed cultures compared to the single cultures. Titratable acidity of the mixed cultures was obviously enhanced compared with that of the single cultures, except for the combinations of Lactobacillus reuteri 940B3 with Saccharomyces cerevisiae 4C and Lactobacillus helveticus 130B4 with Candida kefyr 2Y305. C. kefyr 2Y305 produced large amounts of ethanol (maximum 1.35g/L), whereas non-lactose-fermenting S. cerevisiae 4C produced large amounts of ethanol only in the mixed cultures. Total glucose and galactose content increased while lactose content decreased in the single cultures of Leuconostoc mesenteroides 6B2081 and Lb. helveticus 130B4. However, both glucose and galactose were completely consumed and lactose was markedly reduced in the mixed cultures with yeasts. The result suggests that yeasts utilize glucose and galactose produced by LAB lactase to promote cell growth. © 2012 Japanese Society of Animal Science.

DOI： 10.1111/j.1740-0929.2012.01035.x

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Lactobacillus gasseriの産生するバクテリオシンを用いた食品保蔵. 招待査読

荒川健佑, 川井泰, 齋藤忠夫

ミルクサイエンス 62 131 - 137 2013年