論文 - 荒川　健佑

A novel C-terminal truncated bacteriocin found by comparison between Leuconostoc mesenteroides 406 and 213M0 isolated from Mongolian traditional fermented milk, airag. 査読

Hasiqimuge, Chihiro Hano, Kensuke Arakawa, Saki Yoshida, Junliang Zhao, Hidehiro Toh, Hidetoshi Morita, Taku Miyamoto

Microorganisms   12   1781   2024年

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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伝統味噌由来乳酸菌が産生する細胞外多糖の産業利用に向けて. 招待

荒川健佑, 村上允唯

公益社団法人日本農芸化学会助成金受領者報告書 (2024)   22 - 25   2024年

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担当区分：筆頭著者,　責任著者   記述言語：日本語   掲載種別：研究論文（研究会，シンポジウム資料等）  

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Differential effects of orally administered Lactobacillus acidophilus L-55 on the gene expression of cytokines and master immune switches in the ileum and spleen of laying hen with an attenuated Newcastle disease virus vaccine. 査読

Hung Hoang Son PHAM, Yusuke FUJII, Kensuke ARAKAWA, Toshimitsu HATABU

Bioscience of Microbiota, Food and Health   41   12 - 19   2022年

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BMFH Press  

DOI： 10.12938/bmfh.2021-026

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乳業用乳酸菌Lactobacillus helveticusの菌体外プロテアーゼ遺伝子保有パターンが乳発酵に及ぼす影響. 招待

荒川健佑

2021年度 奨励研究報告書 一般財団法人東洋水産財団   135 - 140   2022年

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担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（研究会，シンポジウム資料等）  

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Dysbiosis in the Salivary Microbiome Associated with IgA Nephropathy—‍A‍ ‍Japanese Cohort Study 査読

Anushka Khasnobish, Lena Takayasu, Ken-ichi Watanabe, Tien Thi Thuy Nguyen, Kensuke Arakawa, Osamu Hotta, Kensuke Joh, Akiyo Nakano, Shuhei Hosomi, Masahira Hattori, Wataru Suda, Hidetoshi Morita

Microbes and Environments   36   ME21006   2021年

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japanese Society of Microbial Ecology  

DOI： 10.1264/jsme2.me21006

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Homologous expression and characterization of gassericin T and gassericin S, a novel class IIb bacteriocin produced by Lactobacillus gasseri LA327. 査読

Genki Kasuga, Masaru Tanaka, Yuki Harada, Hiroshi Nagashima, Taisei Yamato, Ayaka Wakimoto, Kensuke Arakawa, Yasushi Kawai, Jan Kok, Tetsuya Masuda

Applied and Environmental Microbiology   85   e02815-18   2019年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Long-term cultivation of Lactobacillus gasseri LA39 in reconstituted skim milk and productivity of the circular bacteriocin “gassericin A”. 査読

Ayaka Wakimoto, Atsushi Shirata, Mayu Saitoh, Yuki Harada, Hiroshi Nagashima, Taisei Yamato, Genki Kasuga, Kensuke Arakawa, Yasushi Kawai, Tetsuya Masuda

Milk Science   68   30 - 36   2019年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Fecal metabolite of a gnotobiotic mouse transplanted with gut microbiota from a patient with Alzheimer’s disease. 査読

Yusuke Fujii, Thuy Tien Thi Nguyen, Yuta Fujimura, Naotaka Kameya, Shoji Nakamura, Kensuke Arakawa, Hidetoshi Morita

Bioscience, Biotechnology, and Biochemistry   83   2144 - 2152   2019年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Draft genome sequence of Butyricimonas faecihominis 30A1, isolated from feces of a Japanese Alzheimer’s disease patient. 査読

Tien Thi Thuy Nguyen, Kenshiro Oshima, Hidehiro Toh, Anushka Khasnobish, Yusuke Fujii, Kensuke Arakawa, Hidetoshi Morita

Microbiology Resource Announcements   8   e00462-19   2019年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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乳酸菌菌体外プロテアーゼによる卵白タンパク質の分解と機能性ペプチドの生成について. 招待

荒川健佑

平成29年度 研究報告概要集 一般財団法人旗影会   46 - 51   2018年

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担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（その他学術会議資料等）  

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Microbiota community structure in traditional fermented milk dadiah in Indonesia: Insights from high-throughput 16S rRNA gene sequencing. 査読

Ade Sukma, Hidehiro Toh, Nguyen Thi Thuy Tien, Najmiatul Fitria, Iyo Mimura, Ryo Kaneko, Kensuke Arakawa, Hidetoshi Morita

Milchwissenschaft - Milk Science International   71   1 - 3   2018年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Cultivable butyrate-producing bacteria of elderly Japanese diagnosed with Alzheimer’s disease. 査読

Thi Thuy Tien Nguyen, Yuta Fujimura, Iyo Mimura, Yusuke Fujii, Ngoc Luong Nguyen, Kensuke Arakawa, Hidetoshi Morita

Journal of Microbiology   56   760 - 771   2018年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Development of an MRS broth-based complete food-grade medium for Lactobacillus gasseri cultivation using food-grade yeast extract and bacteriocin produced by the bacteria. 査読

Genki Kasuga, Fumika Togashi, Maiko Abe, Masaru Tanaka, Kensuke Arakawa, Yasushi Kawai, Taku Miyamoto, Tetsuya Masuda

Milk Science   66   195 - 204   2017年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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抗菌効果と保健効果を有した機能性発酵液卵の開発および評価. 招待

荒川健佑

平成28年度 研究報告概要集 一般財団法人旗影会   48 - 55   2017年

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担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（その他学術会議資料等）  

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Production of a bacteriocin-like inhibitory substance by Leuconostoc mesenteroides subsp dextranicum 213M0 isolated from Mongolian fermented mare milk, airag 査読

Kensuke Arakawa, Saki Yoshida, Hiroki Aikawa, Chihiro Hano, Tsognemekh Bolormaa, Sedkhuu Burenjargal, Taku Miyamoto

ANIMAL SCIENCE JOURNAL   87 ( 3 )   449 - 456   2016年3月

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY  

Strain 213M0 was selected with productivity of a bacteriocin-like inhibitory substance (BLIS) among 235 strains of lactic acid bacteria (LAB) isolated from Mongolian fermented milk airag'. Strain 213M0 was species-identified as Leuconostoc mesenteroides subsp. dextranicum by morphological observation, carbohydrate fermentation profiling and sequencing the 16S rRNA gene. Incubation temperature proper to produce the BLIS was 25 degrees C rather than 30 and 37 degrees C, and the production actively proceeded during the exponential growth phase of the producer cells. Antibacterial effect of BLIS 213M0 was limited to all nine strains of Listeria sp. bacteria and seven strains of LAB cocci among 53 tested strains, which corresponds to a typical feature of the class IIa pediocin-like bacteriocins. BLIS 213M0 was not inactivated in every broad pH range solution (pH 2.0-11.0), and was stable against storage at 25 degrees C for 1 week and heating at 121 degrees C for 15 min under pH 4.5. Peptide frame of BLIS 213M0 was confirmed by inactivation with some peptidases, and then its molecular weight was estimated to be 2.6-3.0 kDa using an in situ activity assay following sodium dodecyl sulfate polyacrylamide gel electrophoresis. The estimated size was different from the other Leuconostoc bacteriocins already reported. These results suggest that BLIS 213M0 would be a novel listericidal bacteriocin.

DOI： 10.1111/asj.12445

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Draft Genome Sequence of Coccoid Lactobacillus equigenerosi NRIC 0697 T Isolated from the Gastrointestinal Tracts of Healthy Thoroughbreds 査読

Hidehiro Toh, Akiyo Nakano, Co Thi Kim Nguyen, Iyo Mimura, Kensuke Arakawa, Kosuke Tashiro, Takefumi Kikusui, Hidetoshi Morita

Genome Announcements   4 ( 1 )   e01679-15   2016年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：American Society for Microbiology  

<italic>Lactobacillus equigenerosi</italic>
NRIC 0697
^T
was isolated from the gastrointestinal tracts of healthy thoroughbreds. This strain produced unique spherical or oval cells, which is rare in the genus
<italic>Lactobacillus</italic>
. Here, we report the draft genome sequence of this strain.

DOI： 10.1128/genomea.01679-15

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Isolation of lactic acid bacteria bound to the porcine intestinal mucosa and an analysis of their moonlighting adhesins. 査読

Hideki Kinoshita, Satoko Ohuchi, Kensuke Arakawa, Masamichi Watanabe, Haruki Kitazawa, Tadao Saito

Bioscience of Microbiota, Food and Health   35   185 - 196   2016年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Draft genome sequence of Leuconostoc mesenteroides 406 isolated from traditional fermented mare milk 'airag' in Tuv aimag, Mongolia. 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Chihiro Hano, Saki Yoshida, Tien Thi Thuy Nguyen, Wulijideligen, Kosuke Tashiro, Kensuke Arakawa, Taku Miyamoto

Genome Announcements   4   e00166-16   2016年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Draft genome sequence of Leuconostoc mesenteroides 213M0 isolated from traditional fermented mare milk 'airag' in Bulgan aimag, Mongolia. 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Chihiro Hano, Saki Yoshida, Tsognemekh Bolormaa, Sedkhuu Burenjargal, Co Thi Kim Nguyen, Kosuke Tashiro, Kensuke Arakawa, Taku Miyamoto

Genome Announcements   4   e00178-16   2016年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Draft genome sequence of Streptococcus orisasini SH06 isolated from a healthy thoroughbred gastrointestinal tract. 査読

Misako Takagi, Akiyo Nakano, Hidehiro Toh, Kenshiro Oshima, Kensuke Arakawa, Fumihiko Nakajima, Kosuke Tashiro, Takefumi Kikusui, Fujitoshi Yanagida, Hidetoshi Morita

Genome Announcements   4   e01566-15   2016年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Draft genome sequence of Bifidobacterium lemurum DSM 28807T isolated from the gastrointestinal tracts of ring-tailed lemur (Lemur catta). 査読

Hidehiro Toh, Takehiro Matsubara, Shuta Tomida, Iyo Mimura, Kensuke Arakawa, Takefumi Kikusui, Hidetoshi Morita

Genome Announcements   5   e01656-16   2016年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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上部消化管内優勢乳酸桿菌種のペプチド要求性に関する研究. 招待

荒川健佑

IFO Research Communications   30   127 - 128   2016年

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担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（その他学術会議資料等）  

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Draft Genome Sequence of Bifidobacterium aesculapii DSM 26737 T , Isolated from Feces of Baby Common Marmoset 査読

Hidehiro Toh, Yumiko Yamazaki, Kosuke Tashiro, Shinpei Kawarai, Kenshiro Oshima, Akiyo Nakano, Co Nguyen Thi Kim, Iyo Mimura, Kensuke Arakawa, Atsushi Iriki, Takefumi Kikusui, Hidetoshi Morita

Genome Announcements   3 ( 6 )   e01463-15   2015年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：American Society for Microbiology  

<italic>Bifidobacterium aesculapii</italic>
DSM 26737
^T
was isolated from feces of baby common marmoset. Here, we report the draft genome sequence of this organism. This paper is the first published report of the genomic sequence of
<italic>B. aesculapii</italic>
.

DOI： 10.1128/genomea.01463-15

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Complete genome sequence of Bifidobacterium angulatum JCM 7096(T) isolated from human feces 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Misa Kiuchi, Hiromi Kuroyanagi, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

JOURNAL OF BIOTECHNOLOGY   211   10 - 11   2015年10月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE BV  

Bifidobacterium angulatum JCM 7096T was isolated from human feces. Here we report the complete genome sequence of this organism. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. angulatum strain. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.jbiotec.2015.06.412

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Complete genome sequence of Bifidobacterium catenulatum JCM 1194(T) isolated from human feces 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Naoko Yamashita, Erica Iioka, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

JOURNAL OF BIOTECHNOLOGY   210   25 - 26   2015年9月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE BV  

Bifidobacterium catenulatum JCM 1194(T) was isolated from human feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. catenulatum strain. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.jbiotec.2015.06.415

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Complete genome sequence of Bifidobacterium breve JCM 1192(T) isolated from infant feces 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Emi Omori, Yasue Hattori, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

JOURNAL OF BIOTECHNOLOGY   210   81 - 82   2015年9月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE BV  

Bifidobacterium breve JCM 1192(T) was isolated from infant feces. Here, we report the complete genome sequence of this organism. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.jbiotec.2015.06.414

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Complete genome sequence of Bifidobacterium bifidum JCM 1255(T) isolated from feces of a breast-fed infant 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Chie Shindo, Keiko Komiya, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

JOURNAL OF BIOTECHNOLOGY   210   66 - 67   2015年9月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE BV  

Bifidobacterium bifidum JCM 1255(T) was isolated from feces of a breast-fed infant. Here we report the complete genome sequence of this organism. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.jbiotec.2015.06.413

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Complete genome sequence of Bifidobacterium pseudocatenulatum JCM 1200(T) isolated from infant feces 査読

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Kensuke Arakawa, Yukiko Takayama, Rina Kurokawa, Kageyasu Takanashi, Kenya Honda, Masahira Hattori

JOURNAL OF BIOTECHNOLOGY   210   68 - 69   2015年9月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE BV  

Bifidobacterium pseudocatenulatum JCM 1200(T) was isolated from infant feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. pseudocatenulatum strain. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.jbiotec.2015.06.416

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Lactobacillus gasseri requires peptides, not proteins or free amino acids, for growth in milk 査読

K. Arakawa, K. Matsunaga, S. Takihiro, A. Moritoki, S. Ryuto, Y. Kawai, T. Masuda, T. Miyamoto

JOURNAL OF DAIRY SCIENCE   98 ( 3 )   1593 - 1603   2015年3月

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE INC  

Lactobacillus gasseri is a widespread commensal lactic acid bacterium inhabiting human mucosal niches and has many beneficial effects as a probiotic. However, L. gasseri is difficult to grow in milk, which hurts usability for the food industry. It had been previously reported that supplementation with yeast extract or proteose peptone, including peptides, enables L. gasseri to grow well in milk. In this study, our objective was to confirm peptide requirement of L. gasseri and evaluate efficacy of peptide release by enzymatic proteolysis on growth of L. gassei in milk. Three strains of L. gasseri did not grow well in modified DeMan, Rogosa, Sharpe broth without any nitrogen sources (MRS-N), but addition of a casein-derived peptide mixture, tryptone, promoted growth. In contrast, little effect was observed after adding casein or a casein-derived amino acid mixture, casamino acids. These results indicate that L. gasseri requires peptides, not proteins or free amino acids, among milk-derived nitrogen sources for growth. Lactobacillus gasseri JCM 1131(T) hardly had growth capacity in 6 kinds of milk-based media: bovine milk, human milk, skim milk, cheese whey, modified MRS-N (MRSL-N) supplemented with acid whey, and MRSL-N supplemented with casein. Moreover, treatment with digestive proteases, particularly pepsin, to release peptides made it grow well in each milk-based medium. The pepsin treatment was the most effective for growth of strain JCM 1131(T) in skim milk among the tested food-grade proteases such as trypsin, alpha-chymotrypsin, calf rennet, ficin, bromelain, and papain. As well as strain JCM 1131(T), pepsinolysis of milk improved growth of other L. gasseri strains and some strains of enteric lactobacilli such as Lactobacillus crispatus, Lactobacillus gallinarum, Lactobacillus johnsonii, and Lactobacillus reuteri. These results suggest that some relatives of L. gasseri also use peptides as desirable nitrogen sources, and that milk may be a good supplier of nutritious peptides to enteric lactobacilli including L. gasseri after peptic digestion in the gastrointestinal tract. This is the first report showing peptide requirement of L. gasseri and efficacy of pepsinolysis on the growth of L. gasseri and its relatives in milk. This study would contribute to increasing usability of L. gasseri and its relatives as probiotics in dairy foods.

DOI： 10.3168/jds.2014-8860

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発酵豆乳に含まれる大豆たん白質由来抗菌ペプチドの特性解析. 招待

荒川健佑, 福留香菜子, 豊川直樹, 大森葉子, 宮本拓

大豆たん白質研究   18   204 - 212   2015年

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担当区分：筆頭著者,　責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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乳酸菌あれこれ #62 多様性の話. 招待

荒川健佑

日本乳酸菌学会誌   25   55 - 55   2014年

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担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（その他学術会議資料等）  

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Genetic and biochemical evidence for gassericin T production from Lactobacillus gasseri LA158. 査読

Narimi Yasuta, Kensuke Arakawa, Yasushi Kawai, Takahiro Chujo, Kiyoshi Nakamura, Haruka Suzuki, Yoshiyuki Ito, Junko Nishimura, Yumiko Makino, Shuji Shigenobu, Tadao Saito

Milk Science   63   9 - 17   2014年

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Food preservative potential of gassericin A-containing concentrate prepared from cheese whey culture supernatant of Lactobacillus gasseri LA39 査読

Kiyoshi Nakamura, Kensuke Arakawa, Yasushi Kawai, Narimi Yasuta, Takahiro Chujo, Masamichi Watanabe, Hiroyuki Iioka, Masashi Tanioka, Junko Nishimura, Haruki Kitazawa, Koichi Tsurumi, Tadao Saito

Animal Science Journal   84 ( 2 )   144 - 149   2013年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Gassericin A (GA) is a circular bacteriocin produced by Lactobacillus gasseri LA39. In this study, GA-containing concentrate was prepared using a cross-flow membrane filtration device (30kDa cut-off) from the culture supernatant of Lb.gasseri LA39 cultivated in a cheese whey-based food-grade medium. The bacteriocin activity titer in the concentrate was 16 times as high as that of the culture supernatant and was completely maintained through each incubation at 4°C for 3months, 37°C for 2months, 60°C for 5 h, and 100°C for 30min. The GA-containing concentrate was used with glycine powder to make custard creams, and then four representative strains of custard cream spoilage bacteria (Bacillus cereus, Lactococcus lactis subsp. lactis, Achromobacter denitrificans and Pseudomonas fluorescens) were individually inoculated at c. 103 colony forming units/g in the custard creams. Throughout 30days of incubation at 30°C, all of the inoculated bacteria were completely inhibited by the combination of 5% (w/w) of the GA-containing concentrate and 0.5% (w/w) glycine. This is the first highly practical application of GA to foods as a biopreservative, and the concentration method and the bacteriocin concentrate would contribute to biopreservation of several foods. © 2012 Japanese Society of Animal Science.

DOI： 10.1111/j.1740-0929.2012.01048.x

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Interaction between lactic acid bacteria and yeasts in airag, an alcoholic fermented milk 査読

Sudun, Wulijideligen, Kensuke Arakawa, Mari Miyamoto, Taku Miyamoto

Animal Science Journal   84 ( 1 )   66 - 74   2013年1月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

The interaction between nine lactic acid bacteria (LAB) and five yeast strains isolated from airag of Inner Mongolia Autonomic Region, China was investigated. Three representative LAB and two yeasts showed symbioses were selected and incubated in 10% (w/v) reconstituted skim milk as single and mixed cultures to measure viable count, titratable acidity, ethanol and sugar content every 24h for 1week. LAB and yeasts showed high viable counts in the mixed cultures compared to the single cultures. Titratable acidity of the mixed cultures was obviously enhanced compared with that of the single cultures, except for the combinations of Lactobacillus reuteri 940B3 with Saccharomyces cerevisiae 4C and Lactobacillus helveticus 130B4 with Candida kefyr 2Y305. C. kefyr 2Y305 produced large amounts of ethanol (maximum 1.35g/L), whereas non-lactose-fermenting S. cerevisiae 4C produced large amounts of ethanol only in the mixed cultures. Total glucose and galactose content increased while lactose content decreased in the single cultures of Leuconostoc mesenteroides 6B2081 and Lb. helveticus 130B4. However, both glucose and galactose were completely consumed and lactose was markedly reduced in the mixed cultures with yeasts. The result suggests that yeasts utilize glucose and galactose produced by LAB lactase to promote cell growth. © 2012 Japanese Society of Animal Science.

DOI： 10.1111/j.1740-0929.2012.01035.x

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Lactobacillus gasseriの産生するバクテリオシンを用いた食品保蔵. 招待 査読

荒川健佑, 川井泰, 齋藤忠夫

ミルクサイエンス   62   131 - 137   2013年

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担当区分：筆頭著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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γ-アミノ酪酸高生産性乳酸菌を応用した鶏肉発酵調味液の開発. 査読

宮本拓, 荒川健佑, 栗木隆吉

日本醸造協会誌   108   652 - 659   2013年

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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ヒト小腸優勢乳酸桿菌Lactobacillus gasseriの食品利用. 招待

荒川健佑

日本農芸化学会・第15回中四国支部若手シンポジウム（第5回農芸化学の未来開拓セミナー）講演要旨集   8 - 9   2013年

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担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（研究会，シンポジウム資料等）  

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γ-アミノ酪酸（GABA）を高生産する乳酸菌の選抜と鶏肉発酵調味液のGABA富化. 査読

侯歌川, 藤川皓江, 荒川健佑, 宮本拓

日本食品科学工学会誌   60   125 - 132   2013年

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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Lactobacillus gasseriの生育因子とバクテリオシン生産. 招待

荒川健佑

ミルクサイエンス   62   45 - 46   2013年

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担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（研究会，シンポジウム資料等）  

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Production of bacteriocin by Leuconostoc mesenteroides 406 isolated from Mongolian fermented mare’s milk, airag. 査読

Wulijideligen, Takayuki Asahina, Kazushi Hara, Kensuke Arakawa, Hiroyuki Nakano, Taku Miyamoto

Animal Science Journal   83 ( 10 )   704 - 711   2012年10月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/j.1740-0929.2012.01010.x

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Detection of platypus-type l/d-peptide isomerase activity in aqueous extracts of papaya fruit 査読

Kensuke Arakawa, Jennifer M. S. Koh, Ben Crossett, Allan M. Torres, Philip W. Kuchel

BIOTECHNOLOGY LETTERS   34 ( 9 )   1659 - 1665   2012年9月

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER  

Peptide isomerase catalyses the post-translational isomerisation of the l- to the d-form of an amino acid residue around the N/C-termini of substrate peptides. To date, some peptide isomerases have been found in a limited number of animal secretions and cells. We show here that papaya extracts have weak peptide isomerase activity. The activity was detected in each 30-100 kDa fraction of the flesh and the seed extracts of unripe and ripe papaya fruit. The definitive activity was confirmed in the ripe papaya extracts, but even then it was much less active than that of the other peptide isomerases previously reported. The activity was markedly inhibited by methanol, and partly so by amastatin and diethyl pyrocarbonate. This is the first report of peptide isomerase activity in a plant and suggests that perhaps every living organism may have some peptide isomerase activity.

DOI： 10.1007/s10529-012-0941-4

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Lactobacillus gasseriの生産するバクテリオシンの食品利用へ向けた検討. 招待

荒川健佑

岡山大学農学部学術報告   101   65 - 70   2012年

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担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）  

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HPLC purification and re-evaluation of chemical identity of two circular bacteriocins, gassericin A and reutericin 6 査読

K. Arakawa, Y. Kawai, Y. Ito, K. Nakamura, T. Chujo, J. Nishimura, H. Kitazawa, T. Saito

LETTERS IN APPLIED MICROBIOLOGY   50 ( 4 )   406 - 411   2010年4月

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-BLACKWELL PUBLISHING, INC  

Aim:
The study aimed for the complete purification and recharacterization of the highly hydrophobic circular bacteriocins, gassericin A and reutericin 6.
Methods and Results:
Gassericin A and reutericin 6 were purified to homogeneity using previously described method and reverse-phase HPLC with an octyl column and eluents of aqueous acetonitrile and 2-propanol. Mass analysis, N-terminal sequencing and bacteriocin assay of the HPLC-purified bacteriocins showed the two bacteriocins had identical seamless circular structures with the same m/z value (5651) of [M + H]+ and both had the same specific activity. d/l-amino acid composition analysis using two distinct methods with the chiral fluorescent derivatization reagents (+)-1-(9-fluorenyl)ethyl chloroformate and o-phthalaldehyde/N-acetyl-l-cystein revealed neither gassericin A nor reutericin 6 contained d-alanine residues contrary to our previous results.
Conclusion:
Purified gassericin A and reutericin 6 are chemically identical circular molecules containing no d-alanine residues.
Significance and Impact of the Study:
The HPLC conditions developed in this study will facilitate advanced purification and correct characterization of other highly hydrophobic bacteriocins.

DOI： 10.1111/j.1472-765X.2010.02810.x

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Conjugative Plasmid from Lactobacillus gasseri LA39 That Carries Genes for Production of and Immunity to the Circular Bacteriocin Gassericin A 査読

Yoshiyuki Ito, Yasushi Kawai, Kensuke Arakawa, Yoshiko Honme, Takashi Sasaki, Tadao Saito

APPLIED AND ENVIRONMENTAL MICROBIOLOGY   75 ( 19 )   6340 - 6351   2009年10月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER SOC MICROBIOLOGY  

Gassericin A is a circular bacteriocin produced by Lactobacillus gasseri strain LA39. We found a 33,333-bp plasmid, designated pLgLA39, in this strain. pLgLA39 contained 44 open reading frames, including seven genes related to gassericin A production/immunity (gaa), as well as genes for replication, plasmid maintenance, and conjugative transfer. pLgLA39 was transferred from LA39 to the type strain of L. gasseri (JCM 1131) by filter mating. The transconjugant exhibited &gt;30-fold-higher more resistance to gassericin A and produced antibacterial activity. Lactobacillus reuteri LA6, the producer of reutericin 6, was proved to harbor a plasmid indistinguishable from pLgLA39 and carrying seven genes 100% identical to gaa. This suggests that pLgLA39 might have been transferred naturally between L. gasseri LA39 and L. reuteri LA6. The seven gaa genes of pLgLA39 were cloned into a plasmid vector to construct pGAA. JCM 1131(T) transformed with pGAA expressed antibacterial activity and resistance to gassericin A. pGAA was segregationally more stable than a pGAA derivative plasmid from which gaaA was deleted and even was more stable than the vector. This suggests the occurrence of postsegregational host killing by the gaa genes. pLgLA39 carried a pemIK homolog, and segregational stabilization of a plasmid by the pLgLA39-type pemIK genes was also confirmed. Thus, pLgLA39 was proved to carry the genes for at least two plasmid maintenance mechanisms, i.e., gaa and pemIK. Plasmids containing a repA gene similar to pLgLA39 repA were distributed in several L. gasseri strains.

DOI： 10.1128/AEM.00195-09

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Effects of gassericins A and T, bacteriocins produced by Lactobacillus gasseri, with glycine on custard cream preservation 査読

K. Arakawa, Y. Kawai, H. Iioka, M. Tanioka, J. Nishimura, H. Kitazawa, K. Tsurumi, T. Saito

JOURNAL OF DAIRY SCIENCE   92 ( 6 )   2365 - 2372   2009年6月

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER DAIRY SCIENCE ASSOC-ADSA  

Lactobacillus gasseri LA39 and LA158 isolated from human-infant feces produce bacteriocins named gassericins A and T, respectively. Both gassericins have high heat stability (121 degrees C, 10 min), good pH tolerance (pH 2-11), and strong bactericidality against many gram-positive bacteria, especially lactic acid bacteria, and thus are expected to be effective food preservatives. A microwell plate assay against 12 strains of custard cream spoilage bacteria showed that the gassericins had broader antibacterial spectra than nisin A. Although the gassericins allowed gram-negative isolates to grow, they successfully inhibited the growth of all tested bacterial strains in microwells with the addition of glycine. Glycine was bacteriostatic against many strains except lactic acid bacteria. For practical use, gassericin A was efficiently produced by cultivation in a food-grade medium improved using cheese whey, nourishing proteose peptone, and surfactant yolk lecithin. The practical preservative effect of gassericin A and glycine was verified from the viability of 4 isolated strains, Bacillus cereus, Lactococcus lactis ssp. lactis, Achromobacter denitrificans, and Pseudomonas fluorescens, in custard creams. Custard cream containing 123 arbitrary units of gassericin A per milliliter entirely growth-inhibited the 2 gram-positive strains. In custard cream containing an insufficient amount of gassericin A (49 arbitrary units/mL), the gram-positive strains gradually grew but were completely inhibited by the addition of 0.5% (wt/wt) glycine. The 2 gram-negative strains did not multiply even in the additive-free custard cream, probably because of the unsuitable growth environment. This is the first report showing the combined effect of bacteriocin and glycine and their application for food preservation, which may be helpful for future use in the food industry.

DOI： 10.3168/jds.2008-1240

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DNA Sequencing and Homologous Expression of a Small Peptide Conferring Immunity to Gassericin A, a Circular Bacteriocin Produced by Lactobacillus gasseri LA39 査読

Yasushi Kawai, Joni Kusnadi, Rober Kemperman, Jan Kok, Yoshiyuki Ito, Mikiko Endo, Kensuke Arakawa, Hideaki Uchida, Junko Nishimura, Haruki Kitazawa, Tadao Saito

APPLIED AND ENVIRONMENTAL MICROBIOLOGY   75 ( 5 )   1324 - 1330   2009年3月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER SOC MICROBIOLOGY  

Gassericin A, produced by Lactobacillus gasseri LA39, is a hydrophobic circular bacteriocin. The DNA region surrounding the gassericin A structural gene, gaaA, was sequenced, and seven open reading frames (ORFs) of 3.5 kbp (gaaBCADITE) were found with possible functions in gassericin A production, secretion, and immunity. The deduced products of the five consecutive ORFs gaaADITE have homology to those of genes involved in butyrivibriocin AR10 production, although the genetic arrangements are different in the two circular bacteriocin genes. GaaI is a small, positively charged hydrophobic peptide of 53 amino acids containing a putative transmembrane segment. Heterologous expression and homologous expression of GaaI in Lactococcus lactis subsp. cremoris MG1363 and L. gasseri JCM1131(T), respectively, were studied. GaaI-expressing strains exhibited at least sevenfold-higher resistance to gassericin A than corresponding control strains, indicating that gaaI encodes an immunity peptide for gassericin A. Comparison of GaaI to peptides with similar characteristics found in the circular bacteriocin gene loci is discussed.

DOI： 10.1128/AEM.02485-08

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Negative effect of divalent cations on production of gassericin T, a bacteriocin produced by Lactobacillus gasseri, in milk-based media. 査読

Kensuke Arakawa, Yasushi Kawai, Junko Nishimura, Haruki Kitazawa, Tadao Saito

International Dairy Journal   19   612 - 616   2009年

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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オランダでの第9回乳酸菌シンポジウム（LAB9）に参加して.

木下英樹, 荒川健佑, 川井泰, 齋藤忠夫

ミルクサイエンス   58   21 - 25   2009年

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記述言語：日本語   掲載種別：研究論文（その他学術会議資料等）  

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第9回国際乳酸菌シンポジウム（LAB9）報告 Section F: Preservation, novel enzymes and stress. 招待

荒川健佑, 川井泰

日本乳酸菌学会誌   20   14 - 15   2009年

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担当区分：筆頭著者   記述言語：日本語   掲載種別：研究論文（その他学術会議資料等）  

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Bacteriocin production of probiotic Lactobacillus gasseri LA39 isolated from human feces in milk-based media 査読

Kensuke Arakawa, Yasushi Kawai, Kenji Fujitani, Junko Nishimura, Haruki Kitazawa, Ken-ichi Komine, Kenzo Kai, Tadao Saito

ANIMAL SCIENCE JOURNAL   79 ( 5 )   634 - 640   2008年10月

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-BLACKWELL  

The use of bacteriocins from Lactobacillus gasseri, a probiotic lactic acid bacterium, as bio-preservatives in the food industry and animal formulations has been limited because few strains of Lb. gasseri are cultivated and produce a bacteriocin in natural media such as milk and milk-based media. By the determination of the growth-supplements to milk among the 47 nutrients, Lb. gasseri JCM1131(T), LA39 and LA158 isolated from human feces were successfully cultured in reconstituted skim milk and cheese whey using proteose peptone as a nutrient supplement, where Lb. gasseri LA39 produced a useful bacteriocin, gassericin A, with effective growth-inhibiting activity against Gram-positive food-borne pathogens. The data suggest these developed low-cost safe media supporting enough production of bacteriocins by the probiotic Lb. gasseri LA39 could be used to improve the safe bio-preservation of foods and therapy of bovine mastitis, and extra cheese whey produced by cheese making industry is reused in the cultivation for probiotics effectively.

DOI： 10.1111/j.1740-0929.2008.00574.x

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Microbial community analysis of food-spoilage bacteria in commercial custard creams using culture-dependent and independent methods 査読

K. Arakawa, Y. Kawai, H. Iioka, M. Tanioka, J. Nishimura, H. Kitazawa, K. Tsurumi, T. Saito

JOURNAL OF DAIRY SCIENCE   91 ( 8 )   2938 - 2946   2008年8月

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE INC  

Custard cream is made from highly nutritive raw materials such as milk and sugar and is easily spoiled by the multiplication of specific microbial contaminants or residents. However, this spoilage microbial community has not been studied. We determined the spoilage microbiota in commercial custard creams using culture-dependent and independent methods. Using the culture-dependent analysis with various agar media, 185 bacterial colonies and 43 eukaryal colonies were isolated from 7 commercial custard cream products. All bacterial isolates were morphologically, physiologically, and genetically identified as bacilli, staphylococci, lactic acid bacteria, and psychrotrophic gram-negative rods. Using culture-independent molecular analysis, the PCR-denaturing gradient gel electrophoresis technique, spoilage of the commercial custard creams was found to be caused by bacilli, staphylococci, lactic acid bacteria, psychrotrophic gram-negative rods, Anoxy-bacillus sp., Caurobacter sp., and Streptococcus sp. bacteria. The detected spoilage bacteria were the same species as previously detected in spoiled milk products and shown in other reports, suggesting that spoilage bacteria in a raw material easily grow in processed foods made from milk. We determined the spoilage microbial communities in commercial custard creams, and these are the first data concerning spoilage microbiota in nonfermented processed foods using a culture-independent analysis. Our study will be useful for the manufacture and safe preservation of dairy products because the first step toward safe food preservation by food manufacturers is to understand the spoilage microbiota in a target food to select optimal preservatives and to reduce the use of food additives.

DOI： 10.3168/jds.2007-0677

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第4回アジア乳酸菌会議および第3回乳酸菌と健康に関する国際シンポジウムに参加して.

荒川健佑, 川井泰, 齋藤忠夫

ミルクサイエンス   56   93 - 97   2008年

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担当区分：筆頭著者   記述言語：日本語   掲載種別：研究論文（その他学術会議資料等）  

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ヒト腸管系乳酸菌が生産するガセリシンA/Tの構造と機能特性. 査読

川井泰, 高橋涼子, 荒川健佑, 齋藤忠夫

日本乳酸菌学会誌   17   32 - 39   2006年

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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環状バクテリオシンの発見とその利用性. 査読

荒川健佑, 川井泰, 齋藤忠夫

日本乳酸菌学会誌   16   67 - 74   2005年

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担当区分：筆頭著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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Structural and functional differences in two cyclic bacteriocins with the same sequences produced by lactobacilli 査読

Y Kawai, Y Ishii, K Arakawa, K Uemura, B Saitoh, J Nishimura, H Kitazawa, Y Yamazaki, Y Tateno, T Itoh, T Saitol

APPLIED AND ENVIRONMENTAL MICROBIOLOGY   70 ( 5 )   2906 - 2911   2004年5月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER SOC MICROBIOLOGY  

Lactobacillus gasseri LA39 and L. reuteri LA6 isolated from feces of the same human infant were found to produce similar cyclic bacteriocins (named gassericin A and reutericin 6, respectively) that cannot be distinguished by molecular weights or primary amino acid sequences. However, reutericin 6 has a narrower spectrum than gassericin A. In this study, gassericin A inhibited the growth of L. reuteri LA6, but reutericin 6 did not inhibit the growth of L. gasseri LA39. Both bacteriocins caused potassium ion efflux from indicator cells and liposomes, but the amounts of efflux and patterns of action were different. Although circular dichroism spectra of purified bacteriocins revealed that both antibacterial peptides are composed mainly of alpha-helices, the spectra of the bacteriocins did not coincide. The results Of D- and L-amino acid composition analysis showed that two residues and one residue of D-Ala were detected among 18 Ala residues of gassericin A and reutericin 6, respectively. These findings suggest that the different D-alanine contents of the bacteriocins may cause the differences in modes of action, amounts of potassium ion efflux, and secondary structures. This is the first report that characteristics of native bacteriocins produced by wild lactobacillus strains having the same structural genes are influenced by a difference in D-amino acid contents in the molecules.

DOI： 10.1128/AEM.70.5.2906-2911.2004

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Heterologous expression of gassericin A, a bacteriocin produced by Lactobacillus gasseri LA39 査読

Yasushi Kawai, Kensuke Arakawa, Ayako Itoh, Boku Saitoh, Yasuyuki Ishii, Junko Nishimura, Haruki Kitazawa, Takatoshi Itoh, Tadao Saito

Animal Science Journal   74 ( 1 )   45 - 51   2003年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Gassericin A, a bacteriocin produced by Lactobacillus gasseri LA39, has a cyclic structure linking N- and C-terminal amino acids. Gassericin A was expressed in Escherichia coli JM109 as a biotinylated fusion protein on the basis of the DNA sequence of mature bacteriocin. A positive clone accumulated the bacteriocin, with no activity, as a soluble fusion protein in the cytoplasm. After release of an N-terminal tag with factor Xa protease, gassericin A was converted into an active peptide having N- and C-termini. The total amount of purified bacteriocins (expressed and native) was 480 μg/L and 370 μg/L, respectively. However, the specific activity of expressed gassericin A was 15 AU/mg lower than that of native bacteriocin (2600 AU/mg). Although the actual Mr (molecular weight) of the expressed bacteriocin should be 5666, the peptide showed the same mobility (Mr 3800) in sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as native cyclic gassericin A, suggesting that the expressed peptide retains compact folding of the molecule similar to that of native gassericin A.

DOI： 10.1046/j.1344-3941.2003.00085.x

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