Updated on 2024/10/18

写真a

 
UTSUGI Shigeko
 
Organization
Institute of Plant Science and Resources Assistant Professor
Position
Assistant Professor
External link

Degree

  • Dr. Agriculture ( Okayama University )

Research Interests

  • Plant Molecular Biology

  • 植物分子生物学

Research Areas

  • Life Science / Plant molecular biology and physiology

  • Life Science / Molecular biology

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

Professional Memberships

 

Papers

  • Conserved cis-acting motifs and localization of transcripts and proteins of MFT2 in barley and rice

    Shigeko Utsugi, Hiroyuki Kawahigashi, Akemi Tagiri, Rie Kikuchi, Kohei Mishina, Hiromi Morishige, Shingo Nakamura

    2023.1

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    Publisher：Cold Spring Harbor Laboratory  

    MFT is an important regulator of seed dormancy in flowering plants. A natural mutation in the A-box motif in the promoter of wheat (Triticum aestivum) MFT2 on chromosome 3A (TaMFT2-3A) has been used to prevent pre-harvest sprouting (PHS) in wheat cultivars in East Asia. Previous research using in situ hybridization showed that TaMFT2-3A is primarily expressed in the seed scutellum. In this study, we analyzed the localization of transcripts and encoded proteins for the TaMFT2 homologs from barley (Hordeum vulgare) and rice (Oryza sativa), HvMFT2 and OsMFT2, respectively. RNA in situ hybridization showed that, like the wheat genes, the rice and barley homologs are primarily expressed in the scutellum, indicating that these three MFT2 genes have a common expression pattern during seed development. Analysis of the cis-acting regulatory elements of their promoter sequences showed that the three MFT2 promoters share eight seed-specific cis-acting RY motifs, which are binding sites for B3-domain transcription factors of the AFL-B3 and VIVIPAROUS1/ABI3-LIKE (VAL) families. In addition, we detected tandemly repeated and partially overlapping A-box motifs in the promoters of HvMFT2, TaMFT2-3B, and TaMFT2-3D, possibly explaining why the natural allele of TaMFT2-3A has been employed in breeding. We generated transgenic rice plants expressing nuclear-localized green fluorescent protein (NLS-2xGFP) and OsMFT2-GFP under the control of a 3-kb fragment of the OsMFT2 promoter. Immunohistochemical staining using anti-GFP antibodies mainly detected GFP in the scutellum and scutellar epithelium, which is an important tissue for initiating germination upon seed hydration. We confirmed these results by confocal microscopy of GFP fluorescence in seeds. Our results suggest that MFT2 expression in rice, barley, and wheat might be regulated by a similar network of transcription factors through multi-RY motifs in the MFT2 promoters, with possible roles in scutellar epithelium development.

    DOI： 10.1101/2023.01.17.524348

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  • TaABI5, a wheat homolog of Arabidopsis thaliana ABA insensitive 5, controls seed germination Reviewed

    Shigeko Utsugi, Ikuo Ashikawa, Shingo Nakamura, Mineo Shibasaka

    Journal of Plant Research volume   133 ( 2 )   245 - 256   2020.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER JAPAN KK  

    Abscisic acid (ABA) response element (ABRE)-binding factors (ABFs) are basic region/leucine zipper motif (bZIP) transcription factors that regulate the expression of ABA-induced genes containing ABRE in their promoters. The amino acid sequence of the wheat bZIP protein, TaABI5, showed high homology to that of Arabidopsis ABA insensitive 5 (ABI5). TaABI5 was classified into the clade of ABI5s in Arabidopsis and rice, unlike TRAB1 of rice, Wabi5 of wheat, and HvABI5 of barley in the bZIP Group A family, by a phylogenetic analysis. TaABI5 was strongly expressed in seeds during the late ripening and maturing stages; however, its expression level markedly decreased after germination. An in situ hybridization analysis showed that TaABI5 mRNA accumulated in seed embryos, particularly the scutellum. In a transient assay using wheat aleurone cells, TaABI5 activated the promoter of Em containing ABRE, which is an embryogenesis abundant protein gene, indicating that TaABI5 acts as a transcription factor in wheat seeds. Furthermore, the seeds of transgenic Arabidopsis lines introduced with 35S:TaABI5 exhibited high sensitivity to ABA and the inhibition of germination. The seed dormancy of the transgenic Arabidopsis lines was stronger than that of Col. These results support TaABI5 playing an important role in mature seeds, particularly before seed germination, and acting as a functional ortholog to Arabidopsis ABI5.

    DOI： 10.1007/s10265-020-01166-3

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  • Control of the Water Transport Activity of Barley HvTIP3;1 Specifically Expressed in Seeds.

    Shigeko Utsugi, Mineo Shibasaka, Masahiko Maekawa, Maki Katsuhara

    Plant & cell physiology   56 ( 9 )   1831 - 1840   2015

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：OXFORD UNIV PRESS  

    Tonoplast intrinsic proteins (TIPs) are involved in the transport and storage of water, and control intracellular osmotic pressure by transporting material related to the water potential of cells. In the present study, we focused on HvTIP3;1 during the periods of seed development and desiccation in barley. HvTIP3;1 was specifically expressed in seeds. An immunochemical analysis showed that HvTIP3;1 strongly accumulated in the aleurone layers and outer layers of barley seeds. The water transport activities of HvTIP3;1 and HvTIP1;2, which also accumulated in seeds, were measured in the heterologous expression system of Xenopus oocytes. When they were expressed individually, HvTIP1;2 transported water, whereas HvTIP3;1 did not. However, HvTIP3;1 exhibited water transport activity when co-expressed with HvTIP1;2 in oocytes, and this activity was higher than when HvTIP1;2 was expressed alone. This is the first report to demonstrate that the water permeability of a TIP aquaporin was activated when co-expressed with another TIP. The split-yellow fluorescent protein (YFP) system in onion cells revealed that HvTIP3;1 interacted with HvTIP1;2 to form a heterotetramer in plants. These results suggest that HvTIP3;1 functions as an active water channel to regulate water movement through tissues during the periods of seed development and desiccation.

    DOI： 10.1093/pcp/pcv104

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  • Functional characterization of a novel plasma membrane intrinsic protein2 in barley

    Mineo Shibasaka, Sizuka Sasano, Sigeko Utsugi, Maki Katsuhara

    Plant Signaling and Behavior   7 ( 12 )   2012

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    Language：English   Publishing type：Research paper (scientific journal)  

    Water homeostasis is crucial to the growth and survival of plants. Plasma membrane intrinsic proteins (PIPs) have been shown to be primary channels mediating water uptake in plant cells. We characterized a novel PIP2 gene, HvPIP2
    8 in barley (Hordeum vulgare). HvPIP2
    8 shared 72-76% identity with other HvPIP2s and 74% identity with rice OsPIP2
    8. The gene was expressed in all organs including the shoots, roots and pistil at a similar level. When HvPIP2
    8 was transiently expressed in onion epidermal cells, it was localized to the plasma membrane. HvPIP2
    8 showed transport activity for water in Xenopus oocytes, however its interaction with HvPIP1
    2 was not observed. These results suggest that HvPIP2
    8 plays a role in water homeostasis although further functional analysis is required in future. © 2012 Landes Bioscience.

    DOI： 10.4161/psb.22294

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  • A Wheat Homolog of MOTHER OF FT AND TFL1 Acts in the Regulation of Germination International journal

    Shingo Nakamura, Fumitaka Abe, Hiroyuki Kawahigashi, Kou Nakazono, Akemi Tagiri, Takashi Matsumoto, Shigeko Utsugi, Taiichi Ogawa, Hirokazu Handa, Hiroki Ishida, Masahiko Mori, Kanako Kawaura, Yasunari Ogihara, Hideho Miura

    PLANT CELL   23 ( 9 )   3215 - 3229   2011.9

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER SOC PLANT BIOLOGISTS  

    Seed dormancy is an adaptive mechanism and an important agronomic trait. Temperature during seed development strongly affects seed dormancy in wheat (Triticum aestivum) with lower temperatures producing higher levels of seed dormancy. To identify genes important for seed dormancy, we used a wheat microarray to analyze gene expression in embryos from mature seeds grown at lower and higher temperatures. We found that a wheat homolog of MOTHER OF FT AND TFL1 (MFT) was upregulated after physiological maturity in dormant seeds grown at the lower temperature. In situ hybridization analysis indicated that MFT was exclusively expressed in the scutellum and coleorhiza. Mapping analysis showed that MFT on chromosome 3A (MFT-3A) colocalized with the seed dormancy quantitative trait locus (QTL) QPhs. ocs-3A.1. MFT-3A expression levels in a dormant cultivar used for the detection of the QTL were higher after physiological maturity; this increased expression correlated with a single nucleotide polymorphism in the promoter region. In a complementation analysis, high levels of MFT expression were correlated with a low germination index in T1 seeds. Furthermore, precocious germination of isolated immature embryos was suppressed by transient introduction of MFT driven by the maize (Zea mays) ubiquitin promoter. Taken together, these results suggest that MFT plays an important role in the regulation of germination in wheat.

    DOI： 10.1105/tpc.111.088492

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  • オオムギMIPファミリーの解析

    宇都木繁子, 柴坂三根夫, 且原真木, 前川雅彦

    育種学研究   13   2011

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  • コムギ種子特異的な液胞型アクアポリン(TIP)の解析

    宇都木繁子, 且原真木, 前川雅彦

    育種学研究   11   2009

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  • Biomarkers of green roof vegetation: anthocyanin and chlorophyll as stress marker pigments for plant stresses of roof environments.

    Izumi C. Mori, Shigeko Utsugi, Shigemi Tanakamaru, Akio Tani, Takashi Enomoto, Maki Katsuhara

    J. Environ. Eng. Manage.   19 ( 1 )   21 - 27   2009

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  • Structural and functional properties of Viviparous1 genes in dormant wheat

    Shigeko Utsugi, Shingo Nakamura, Kazuhiko Noda, Masahiko Maekawa

    GENES & GENETIC SYSTEMS   83 ( 2 )   153 - 166   2008.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：GENETICS SOC JAPAN  

    Viviparous 1 (Vp1) of maize is known to encode a transcription factor VP1 that controls seed germination. Hexaploid wheat possesses three Vp1 homoeologues (TaVp1): TaVp-A1, TaVp-B1 and TaVp-D1. In this study, we attempted to characterize the molecular properties of TaVp1 in a highly dormant wheat cultivar, Minamino-komugi (Minamino). The seeds of Minamino showed much higher sensitivity to the inhibitory effect of ABA on germination than those of non-dormant cultivars, Sanin-1 and Tozan-18. The sequence analyses of cDNAs also revealed that some of TaVp-A1 transcripts and TaVp-D1 transcripts were spliced incorrectly, presumably resulting in production of truncated or deleted proteins. Most TaVp-B1 transcripts were spliced correctly, but some had an additional 3-bp (AAG) insertion in the B3 domain, which may not affect their function. RT-PCR analyses showed that TaVp1 was highly expressed in Minamino embryos in maturing seeds but much less in roots and leaves of seedlings. The level of TaVp1 mRNA was high when the embryos were treated with ABA but markedly decreased in water-imbibed mature embryos whose dormancy had been broken. Expression analyses of the individual homoeologues showed that the level of TaVp-A1 transcripts was highest in embryos of DAP 20 but much lower in the matured embryos. TaVp-B1 was highly expressed in developing and maturing seed embryos, while TaVp-D1 mRNA existed at lower levels in developing embryos but increased as the seeds were matured. These results suggest that the majority of TaVp1, especially TaVp-B1, are properly spliced and may function as a transcription factor playing an important role on dormancy in Minamino. By employing an efficient transient expression system using diploid wheat seeds, we confirmed the dual function of TaVP-B1: the activation of Em expression and the repression of alpha-amylase expression.

    DOI： 10.1266/ggs.83.153

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  • The wheat Rc gene for red coleoptile colour codes for a transcriptional activator of late anthocyanin biosynthesis genes

    Nisar Ahmed, Masahiko Maekawa, Shigeko Utsugi, Kazuhide Rikiishia, Aftab Ahmad, Kazuhiko Noda

    JOURNAL OF CEREAL SCIENCE   44 ( 1 )   54 - 58   2006.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD  

    Genes that regulate anthocyanin pigmentation in wheat coleoptiles were investigated in three lines of wheat. When grown in the light, the wheat line Hope developed red coleoptiles, while another line, Chinese Spring (CS), expressed green coleoptiles. Another line CS(Hope7A), a chromosome substitution line that carries chromosome 7A of Hope in a CS background, also developed a red coleoptile. In the dark, none of the three lines developed colored coleoptiles. Anthocyanin biosynthesis pathway genes have been grouped into two subsets: early biosynthesis genes (EBGs), which include CHS, CHI and F3H, and late biosynthesis genes (LBGs), which include DFR, ANS and UFGT. None of these six genes was upregulated in coleoptiles grown in the dark, whereas all were activated by light in coleoptiles of Hope and CS(Hope7A). In coleoptiles of CS grown in the light, the EBGs were expressed, but the three LBGs were not. The Rc gene located on chromosome 7 appears to encode a transcription activator of LBGs involved in light-regulation of anthocyanin synthesis. (c) 2006 Elsevier Ltd. All rights reserved.

    DOI： 10.1016/j.jcs.2006.03.002

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  • An efficient transient gene expression system using aleurones of diploid wheat seeds

    Shigeko Utsugi, Masahiko Maekawa, Kazuhiko Noda

    Plant Biotechnology   23 ( 4 )   413 - 417   2006

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Plant Cell and Molecular Biology  

    To establish a transient gene expression system for investigating the molecular function of genes in mature wheat seeds, we bombarded embryos or aleurones of three hexaploid wheat (Triticum aestivum L.) varieties Chinese Spring, RL4137 and OS21-5, two diploid wheat (T. monococcum L.) varieties KT3-1 and KT3-5 and a barley (Hordeum vulgare, L.) variety Himalaya with fusion constructs containing the GUS reporter gene driven by the Actin1 promoter or α-amylase promoter using a particle gun, and compared the efficiency of gene expression for the transient assay. The efficiency of transient expression was high in aleurone tissues of OS21-5, KT3-1, KT3-5 and Himalaya. In the aleurone tissues of KT3-5, the a-amylase promoter was especially markedly activated by gibberellic acid (GA) and by a GA-inducible MYB transcription activator of T. monococcum, TmGAMYB. This assay system using the aleurone tissues of KT3-5 may be especially useful for investigating which genes are expressed and how they are regulated in germinating wheat seeds. Copyright © 2006 The Japanese Society for Plant Cell and Molecular Biology.

    DOI： 10.5511/plantbiotechnology.23.413

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  • トウモロコシのアントシアニン合成系遺伝子の制御因子CIとB-peruによる発達中のコムギ幼葉鞘におけるアントシアニンの一過的発現

    Ahmed Nisar, 前川 雅彦, 宇都木 繁子, 氷見 英子, Ablet Hader, 力石 和英, 野田 和彦

    育種学研究   5 ( 1 )   22 - 23   2003.3

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    Language：Japanese   Publisher：日本育種学会  

    トウモロコシのアントシアニン合成系遺伝子の転写制御因子CI B-peruを発達中のコムギ幼葉鞘にパーティクルボンバードメント法で導入した.アントシアニン色素が明確な赤い点として誘導され,その数は幼葉鞘当たり約60に達した.一方,同時に導入したGusA遺伝子では約20の滲んだ青いGUSの点が誘導された.発芽後36時間の幼葉鞘が,これら外来遺伝子の導入発現に最も良い組織であった.また1から5μgのプラスミドDNAで覆った金粒子で充分にアントシアニンやGUSの発現が誘導された.ヘリウムガス圧(900,1100,1300,1500ポンド/インチ^2)或いは停止スクリーンと幼葉鞘間の距離(10,15,20？)は遺伝子導入効率には有意に影響を与えなかった.一方,MS培地に半径1？の円状に並べた芽生えで最も良く遺伝子が導入された.これらの結果は,コムギ幼葉鞘はコムギ遺伝子の一過的発現分析に良い組織であり,またCI B-peruはレポーター遺伝子として使用できることを示した.

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  • Transient expression of anthocyanin in developing wheat Coleoptile by maize C1 and beta-peru regulatory genes for anthocyanin synthesis

    N Ahmed, M Maekawa, S Utsugi, E Himi, H Ablet, K Rikiishi, K Noda

    BREEDING SCIENCE   53 ( 1 )   29 - 34   2003.3

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPANESE SOC BREEDING  

    Maize transcription factors for the genes of anthocyanin synthesis pathway, C1/B-peru, were delivered into developing wheat coleoptiles by a particle bombardment method. Anthocyanin pigments were induced as discrete red spots and their number reached about 60 spots per coleoptile, compared to about 20 diffused blue GUS spots, which were induced by the GusA gene transferred concomitantly. Coleoptiles of seedlings collected 36 h after germination were most suitable tissue for the expression of delivered foreign genes. One to five mug of plasmid DNA for coating gold particles was sufficient for induction of anthocyanin and GUS, indicating that the transferred gene was expressed efficiently in coleoptile cells. Helium gas pressure (900, 1100, 1300 or 1500 pounds per square inch) and distances (10, 15, or 20 cm) between the stopping screen and coleoptile did not affect significantly the efficiency of gene transfer. Seedlings arranged in a circle with a 1-cm radius on a MS medium plate were targeted well by gold particles. The results showed that the wheat coleoptile was a good target tissue for transient assay of wheat genes and that C1/B-peru can be used as a reporter gene.

    DOI： 10.1270/jsbbs.53.29

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  • Transactivation of late anthocyanin biosynthesis genes in developing wheat coleoptiles by maize C1 and B-Peru regulatory genes for anthocyanin synthesis

    AHMED N., UTSUGI S., HIMI E., MAEKAWA M., RIKISHII K., MATSUURA T., NODA K.

    4   170 - 170   2002.8

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  • Structural features of a wheat plastome as revealed by complete sequencing of chloroplast DNA

    Y Ogihara, K Isono, T Kojima, A Endo, M Hanaoka, T Shiina, T Terachi, S Utsugi, M Murata, N Mori, S Takumi, K Ikeo, T Gojobori, R Murai, K Murai, Y Matsuoka, Y Ohnishi, H Tajiri, K Tsunewaki

    MOLECULAR GENETICS AND GENOMICS   266 ( 5 )   740 - 746   2002.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER-VERLAG  

    Structural features of the wheat plastome were clarified by comparison of the complete sequence of wheat chloroplast DNA with those of rice and maize chloroplast genomes. The wheat plastome consists or a 134,545-bp circular molecule with 20,703-bp inverted repeats and the same gene content as the rice and maize plastomes. However, some structural divergence was found even in the coding regions of genes. These alterations are due to illegitimate recombination between two short direct repeats and/or replication slippage. Overall comparison of chloroplast DNAs among the three cereals indicated the presence of some hot-spot regions for length mutations. Whereas the region with clustered tRNA genes and that downstream of rbcL showed divergence in a species-specific manner, the deletion patterns of ORFs in the inverted-repeat regions and the borders between the inverted repeats and the small single-copy region support the notion that wheat and rice are related more closely to cacti other than to maize.

    DOI： 10.1007/s00438-001-0606-9

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  • Characterization of water induced α-amylase (WIA) in de-embryonated half seeds of wheat.

    HARDOR A., RIKIISHI K., UTSUGI S., MATSUURA T., MAEKAWA M., NODA K.

    4 ( 1 )   203 - 203   2002

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  • Wheat Re gene for coleoptile colour regulates the expression of DFR gene in developing coleoptile.

    Ahmed, N, Utsugi, S, Himi, E, Maekawa, M, Rikiishi, K, Matsuura, T, Noda, K

    Breed. Res. 4 (suppl. 1)   2002

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  • 高いαーアミラーゼ活性を示したコムギ除胚半切種子におけるデンプン分解酵素の解析

    ハードル、アブラビ, 力石和英, 宇都木繁子, 松浦恭和, 前川雅彦, 野田和彦

    育種学研究   2001

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  • Characterization of AP2 domain-containing protein gene clones in ESTs derived from wheat young spike

    MURAI K., UEHARA A., HANDA Y., MATSUOKA Y., FUTO S., SATO M., HAYAKAWA K., NODA K., UTSUGI S., TOMITA M., TERACHI T., OGIHARA Y.

    2 ( 2 )   73 - 73   2000.9

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  • Aberrant segregation of 1g observed in F2 of I-102(indica type)//T-65 1g BC10 in rice.

    MAEKAWA M., RIKIISHI K., MATSUURA T., UTSUGI S., NODA K.

    2 ( 2 )   256 - 256   2000.9

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  • Complete sequence of wheat chloroplast DNA. IV. MITE like sequence found in wheat chloroplast DNA

    OGIHARA Y., ISONO K., KOJIMA T., ENDO A., MURAI R., MURAI K., HANAOKA M., SHIINA T., TERACHI T., UTSUGI S., MURATA M., MORI N., TAKUMI S., IKEO K., GOJOBORI T., MATSUOKA Y., OHNISHI Y., TAJIRI H., TSUNEWAKI K.

    2 ( 1 )   30 - 30   2000.4

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  • Cloning and analysis of genes related to gibberellin response in wheat.

    UTSUGI S., NODA K.

    2 ( 1 )   79 - 79   2000.4

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  • α-Amylase activity in de-embryonated half seeds of wheat during seed development

    ABRABI H., MAEKAWA M., UTSUGI S., NODA Kaz.

    2 ( 1 )   310 - 310   2000.4

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  • Non-segregation of albino character in rice plants heterozygous for variegated albino

    MAEKAWA M., RIKIISHI K., MATSUURA T., UTSUGI S., NODA K.

    2 ( 1 )   15 - 15   2000

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  • Chinese spring wheat (Triticum aestivum L.) chloroplast genome: Complete sequence and contig clones

    Y Ogihara, K Isono, T Kojima, A Endo, M Hanaoka, T Shiina, T Terachi, S Utsugi, M Murata, N Mori, S Takumi, K Ikeo, T Gojobori, R Murai, K Murai, Y Matsuoka, Y Ohnishi, H Tajiri, K Tsunewaki

    PLANT MOLECULAR BIOLOGY REPORTER   18 ( 3 )   243 - 253   2000

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：INT SOC PLANT MOLECULAR BIOLOGY  

    Libraries of plasmid clones covering the entire chloroplast (cp) genome of the common wheat, Triticum aestivum cv. Chinese Spring were constructed and assembled into contig-clones. From these, we obtained the complete nucleotide sequence of wheat chloroplast DNA - a 134,540 bp circular DNA (DDBJ accession no. AB042240) containing four species of ribosomal RNA, 30 genes for 20 species of transfer RNA, and 71 protein coding genes. Additionally, we detected five unidentified open reading frames conserved among grasses. Plasmid clones are available on request.

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  • Wheat seed color and expression of the genes in anthocyanin synthesis pathway

    HIMI E., UTSUGI S., RIKIISHI K., MAEKAWA M., NODA K.

    2 ( 1 )   71 - 71   2000

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  • インド型イネI-102//T-65 lg BC10F2に生じたlgの異常分離

    前川雅彦, 力石和英, 松浦恭和, 宇都木繁子, 野田和彦

    育種学研究   2000

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  • イネの斑入りアルビノ形質ヘテロ個体におけるアルビノ形質不分離

    前川雅彦, 力石和英, 松浦恭和, 宇都木繁子, 野田和彦

    育種学研究   2000

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  • Characterization of a flower-specific gene encoding a putative myrosinase binding protein in Arabidopsis thaliana

    K Takechi, W Sakamoto, S Utsugi, M Murata, F Motoyoshi

    PLANT AND CELL PHYSIOLOGY   40 ( 12 )   1287 - 1296   1999.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS  

    A cDNA clone, 4B-1, previously isolated by differential screening is preferentially expressed in floral organs of Arabidopsis thaliana. Characterization of the full length cDNA and the genetic locus corresponding to 4B-1 cDNA revealed that it potentially encodes a myrosinase binding protein (MBP) which is presumably present in a large myrosinase complex. The deduced amino acid sequence of the polypeptide encoded by cDNA clone (designated f-AtMBP) appeared to consist of two parts: one region at the C-terminal half representing overall homology with AtMBP, an MBP homologue in A. thaliana, and the other at an extended N-terminal region of about 150 amino acids showing significant identity with the N-terminal region of the MBP-related protein reported in Brassica. Expression analysis by RNA blot and in situ hybridization showed that f-AtMBP was specifically expressed in floral meristems, pistils, stamens, petals, and ovules of immature flowers, but no expression was observed in the specialized cells called the myrosin cells in the hypocotyl and cotyledons of developing seeds where myrosinase enzymes are normally found. Although MBPs and MBP-related proteins are considered to be inducible by exogenous application of signal molecules and physical wounding, we found that f-AtMBP expression was not activated by such treatment, suggesting that f-AtMBP is a novel type of MBP specific to floral organs.

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  • コムギ幼穂におけるexpressed Sequence Tags (EST)の大量解析

    荻原保成, 藤田雅子, 川浦香奈子, 大澤智子, 村井耕二, 松岡由浩, 布藤聡, 佐藤恵美, 早川克志, 野田和彦, 宇都木繁子, 力石和英, Ahmed Nisar

    育種学研究   1999

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  • Putative alternative splicing occurred in XT2, a transcription factor for anthocyanin pigmentation in rice.

    景山圭祐, 大森拓, 前川雅彦, 力石和英, 宇都木繁子, 野田和彦, 坂本亘, 本吉総男

    育種学研究   1   1999

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  • Arabidopsis thaliana vegetative storage protein (VSP) genes: gene organization and tissue-specific expression

    S Utsugi, W Sakamoto, M Murata, F Motoyoshi

    PLANT MOLECULAR BIOLOGY   38 ( 4 )   565 - 576   1998.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：KLUWER ACADEMIC PUBL  

    We have previously identified two cDNAs encoding vegetative storage proteins (VSPs) in Arabidopsis thaliana. Unlike soybean in which VSPs accumulate at high levels in leaves, A. thaliana VSP mRNAs are abundant in flowers. To understand tissue-specific expression and possible roles of VSPs on reproductive organ development, genes corresponding to VSPs (Vsp1 and Vsp2) and their putative promoters were characterized in this study. Genomic sequence analysis revealed that Vsp1 and Vsp2 resemble each other except in their introns, and that these two genes were organized in a tandem array with an interval of 6 kb in a region. The expression patterns of Vsp1 and Vsp2 were examined using transgenic A. thaliana plants carrying a promoter from Vsp1 or Vsp2 fused to a bacterial B-glucuronidase (GUS) reporter gene. The promoter from Vsp1 expressed its effect in gynoecia, especially in styles, the basal and distal ends of ovaries and in siliques, whereas the promoter from Vsp2 showed its activity in vegetative shoots, petioles, peduncles and receptacles of floral organs. These results suggest that expression of Vsp1 and Vsp2 may be developmentally regulated in A. thaliana. In the transgenic plants, the GUS activity was induced by wounding in an area around the mid-rib of leaves. Therefore, Vsp1 and Vsp2 promoters appear to have elements required for both tissue specificity and wounding.

    DOI： 10.1023/A:1006072014605

    Web of Science

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  • Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

    S Utsugi, W Sakamoto, Y Ogura, M Murata, F Motoyoshi

    PLANT MOLECULAR BIOLOGY   32 ( 4 )   759 - 765   1996.11

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    Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross-hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.

    DOI： 10.1007/BF00020217

    Web of Science

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Books

  • 雑草学辞典

    日本雑草学会  2010 

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MISC

  • Controls of the water transport activities of Arabidopsis tonoplast intrinsic proteins 3, AtTIP3s and the effects on seed development and germination

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)   64th   2023

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  • Analysis of CO2 transport activity of plasma membrane-associated aquaporins of tomato

    光本晏理, 宇都木繁子, 土屋善幸, 且原真木, 森泉

    日本植物生理学会年会(Web)   64th   2023

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  • Controls of the water transport activities of Arabidopsis tonoplast intrinsic proteins 3, AtTIP3s

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)   63rd   2022

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  • Analysis of the C-terminal region controlled thewater transport activities of Arabidopsistonoplast intrinsic proteins, AtTIP3s

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)   62nd   2021

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  • Water transport activities of Arabidopsis tonoplast intrinsic proteins, AtTIPs

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)   61st   2020

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  • オオムギ種子における液胞膜型アクアポリン(HvTIPs)の役割

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)   60th   2019

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  • 種子で発現するオオムギ液胞膜型アクアポリン(HvTIPs)のタンパク質間相互作用

    宇都木繁子, 柴坂三根夫, 且原真木

    日本植物生理学会年会(Web)   59th   2018

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  • オオムギ種子における液胞膜型アクアポリンの水輸送活性の調節

    宇都木繁子, 柴坂三根夫, 且原真木

    日本植物生理学会年会要旨集   55th   2014

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  • 種子特異的に発現するオオムギTIP3;1による水輸送活性の調節

    宇都木繁子, 篠野静香, 柴坂三根夫, 且原真木

    日本植物生理学会年会要旨集   54th   2013

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  • 原形質膜型アクアポリンPIP1とPIP2の共発現による活性抑制

    柴坂三根夫, 篠野静香, 宇都木繁子, 且原真木

    日本植物生理学会年会要旨集   54th   2013

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  • Identification of genes controlling seed dormancy in wheat Invited

    Shingo Nakamura, Hiroyuki Kawahigashi, Kou Nakazono, Shigeko Utsugi, Yashunori Ogihara

    NARO Press Release   2011.12

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

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  • The DNA marker that can detect wheat pre-harvest sprouting tolerance MFT gene Invited Reviewed

    Shingo Nakamura, Fumitaka Abe, Hiroyuki Kawahigashi, Kou Nakazono, Akemi Tagiri, Takashi Matsumoto, Shigeko Utsugi, Taiichi Ogawa, Hirokazu Handa, Hiroki Ishida, Masahiko Mori, Kanako Kawaura, Yasunari Ogihara, Makiko Chono, Ikuo Ashikawa, Hideho Miura

    NARO Achievement information 2011   2011   2011.12

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    Language：Japanese   Publishing type：Internal/External technical report, pre-print, etc.  

    J-GLOBAL

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  • Identification of wheat germination suppressor gene MFT Invited Reviewed

    Shingo Nakamura, Fumitaka Abe, Hiroyuki Kawahigashi, Kou Nakazono, Akemi Tagiri, Takashi Matsumoto, Shigeko Utsugi, Taiichi Ogawa, Hirokazu Handa, Hiroki Ishida, Masahiko Mori, Kanako Kawaura, Yasunari Ogihara, Makiko Chono, Ikuo Ashikawa, Hideho Miura

    NARO Achievement information 2011   2011   2011.12

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    Language：Japanese   Publishing type：Internal/External technical report, pre-print, etc.  

    J-GLOBAL

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  • Cooling effect on buildings by the roof greening at Research Institute for Bioresources, Okayama University.

    Katsuhatra, M, Tanakamaru, S, Mori, I.C, Tani, A, Utsugi,S, Enomoto, T, Maitani, T

    Environment Research and Control   31 ( 1 )   21 - 25   2009

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  • Cooling effect on buildings by the roof greening at Research Institute for B Bioresources, Okayama University

    Environmental research & control   31 ( 31 )   21 - 25   2009

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  • 種子のアブシジン酸応答に関わるコムギbZIPタンパク質の解析

    宇都木繁子, 中村信吾, 前川雅彦

    日本分子生物学会年会講演要旨集   28th   2005

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  • Sugar suppression of alfa-glucosidase activity in wheat embryos

    Rikiishi, K, Sugimoto, M, Maetani, M, Matsuura T, Utsugi, S, Maekawa, M, Noda, K

    Proc. 9th Int. Symp. Pre-Harvest Sprouting in Creals   2001

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  • ジベレリンにより誘導されるコムギGAmyb遺伝子のクローニングと解析

    宇都木繁子, 前川雅彦, 野田和彦

    日本分子生物学会年会プログラム・講演要旨集   23rd   2000

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  • A system for transient assey of transcriptional factors controlling anthocyanin biosynthesis at the aleurone layer of mature rice seed

    Maekawa, M, Kageyama, K, Rikiishi, K, Utsugi, S, Noda, K, Sakamoto, W

    Breeding Science   2   181 - 187   2000

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  • A System for Transient Assay of Transcriptional Factors Controlling Anthocyanin Biosynthesis at the Aleurone Layer of Mature Rice Seed

    MAEKAWA Masahiko, KAGEYAMA Keisuke, RIKIISHI Kazuhide, UTSUGI Shigeko, NODA Kazuhiko, SAKAMOTO Wataru

    Breeding Research   2 ( 4 )   181 - 187   2000

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    Language：Japanese   Publisher：Japanese Society of Breeding  

    Various combinations of temperature and time for soaking seed were tested to establish a system for transient assay of transcriptional factors controlling anthocyanin biosynthesis to prove that the isolated gene is an inducible gene for anthocyanin expression at the aleurone layer of mature rice seed. The aleurone of mature seed of RIB-Pl with purple pericarp was sufficiently exposed under five combinations. Both Cl and B-Peru, transcriptional factors controlling anthocyanin biosynthesis of maize, were introduced by particle bombardment into the aleurone exposed under these five conditions. The highest expression of anthocyanin was obtained at the aleurone of the seeds soaked at 28°C for 4 hours. The effect of concentration of plasmids with Cl and B-Peru for introduction into the aleurone of A-58 mature seed with white pericarp soaked at 28°C for 4 hours was examined. Both Cl and B-Peru were found to give high expression of anthocyanin at the same concentration. Especially, 5 μg of Cl with 5 μg of B-Peru and 5 lig of Cl with 10 μg of B-Peru showed the highest expression. In conclusion, soaking of mature rice seed at 28°C for 4 hours and introduction of 5 lig of Cl with 51μg of B-Peru into the exposed aleurone was found to be a highly efficient system for transient assay of transcriptional factors controlling anthocyanin biosynthesis.

    DOI： 10.1270/jsbbr.2.181

    CiNii Article

    CiNii Books

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    Other Link： https://agriknowledge.affrc.go.jp/RN/2010620894

  • Complete sequence of wheat chloroplast DNA

    OGIHARA Yasunari, ISONO Kazuhiro, KOJIMA Toshio, ENDO Akira, MURAI Rika, MURAI Koji, HANAOKA Mitsumasa, SHIINA Takashi, TERACHI Toru, UTSUGI Shigeko, MURATA Minoru, MORI Naoki, TAKUMI Shigeo, IKEO Kazuho, GOJOBORI Takashi, MATSUOKA Yoshihiro, OHNISHI Yukari, TAJIRI Hikaru, TSUNEWAKI Koichiro

    育種学研究   21   481 - 481   1998.12

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  • Analysis of cDNA clone on flower vase peculiar gene expression in Arabidopsis.

    武智克彰, 宇都木繁子, 小倉豊, 坂本亘, 村田稔, 本吉総男

    日本遺伝学会大会プログラム・予稿集   70th   1998

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  • Decision of all structures of wheat chloroplast genome II. Entire base arrangement of wheat chloroplast DNA.

    荻原保成, 磯野一宏, 村井里佳, 華岡光正, 寺地徹, 宇都木繁子, 森直樹, 池尾一穂, 常脇恒一郎

    日本遺伝学会大会プログラム・予稿集   70th   1998

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  • コムギ葉緑体DNAの全塩基配列決定

    荻原保成, 磯野一宏, 村井里佳, 華岡光正, 寺地徹, 宇都木繁子, 森直樹, 池尾一穂, 常脇恒一郎

    日本分子生物学会年会プログラム・講演要旨集   21st   1998

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  • Analysis of Arabidopsis Vsp gene.

    宇都木繁子, 村田稔, 坂本亘, 小倉豊, 本吉総男

    日本遺伝学会大会プログラム・予稿集   69th   1997

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  • Analysis of CDNA clone on flower vase peculiar gene expression in Arabidopsis.

    武智克彰, 宇都木繁子, 小倉豊, 坂本亘, 村田稔, 本吉総男

    日本遺伝学会大会プログラム・予稿集   69th   1997

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  • Analysis of flowering organ specific genes in Arabidopsis.

    宇都木繁子, 坂本亘, 小倉豊, 村田稔, 本吉総男

    日本遺伝学会大会プログラム・予稿集   67th   1995

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Presentations

  • Carbon dioxide transport by Arabidopsis PIP2 aquaporins

    Tania, S.S, Utsugi, S, Tsuchiya, Y, Sasano, S, Katsuhara, M, Mori, I

    Taiwan-Japan Plant Biology 2023  2023.10.13 

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    Event date： 2023.10.13 - 2023.10.16

    Presentation type：Poster presentation  

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  • Conserved cis-acting motifs and localization of transcripts and proteins of MFT2 from barley and rice

    Shigeko Utsugi, Hiroyuki Kawahigashi, Akemi Tagiri, Rie Nakagawa-Kikuchi, Kohei Mishina, Hiromi Morishige, Shingo Nakamura

    15th International Symposium on Pre-harvest Sprouting in Cereals  2023.10.5 

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    Event date： 2023.10.4 - 2023.10.6

    Language：English   Presentation type：Poster presentation  

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  • Analysis of CO2 transport activity of plasma membrane-associated aquaporins of tomato

    Anri Mitsumoto, Shigeko Utsugi, Yoshiyuki Tsuchiya, Maki Katsuhara, Izumi Mori

    2023.3.17 

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    Event date： 2023.3.10 - 2023.3.17

    Language：Japanese   Presentation type：Poster presentation  

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  • Controls of the water transport activities of Arabidopsis tonoplast intrinsic proteins 3, AtTIP3s and the effects on seed development and germination

    UTSUGI SHIGEKO, KATSUHARA MAKI

    2023.3.17 

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    Event date： 2023.3.10 - 2023.3.17

    Language：Japanese   Presentation type：Poster presentation  

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  • シロイヌナズナ液胞膜型アクアポリンAtTIP3ファミリーの水輸送活性の制御

    宇都木繁子, 且原真木

    第63回日本植物生理学会年会  2022.3.24 

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    Event date： 2022.3.22 - 2022.3.24

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  • Analysis of the C-terminal region controlled thewater transport activities of Arabidopsistonoplast intrinsic proteins, AtTIP3s

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)  2021 

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    Event date： 2021

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  • Water transport activities of Arabidopsis tonoplast intrinsic proteins, AtTIPs

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)  2020 

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    Event date： 2020

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  • オオムギ種子における液胞膜型アクアポリン(HvTIPs)の役割

    宇都木繁子, 且原真木

    日本植物生理学会年会(Web)  2019 

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    Event date： 2019

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  • 種子で発現するオオムギ液胞膜型アクアポリン(HvTIPs)のタンパク質間相互作用

    宇都木繁子, 柴坂三根夫, 且原真木

    日本植物生理学会年会(Web)  2018 

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    Event date： 2018

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  • オオムギ種子における液胞膜型アクアポリンの水輸送活性の調節

    宇都木繁子, 柴坂三根夫, 且原真木

    日本植物生理学会年会要旨集  2014 

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    Event date： 2014

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  • Identification of genes controlling seed dormancy in wheat Invited

    Shingo Nakamura, Hiroyuki Kawahigashi, Kou Nakazono, Shigeko Utsugi, Yashunori Ogihara

    NARO Press Release  2011.12.6 

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    Event date： 2011.12.6

    Language：Japanese  

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  • The DNA marker that can detect wheat pre-harvest sprouting tolerance MFT gene Invited

    Shingo Nakamura, Fumitaka Abe, Hiroyuki Kawahigashi, Kou Nakazono, Akemi Tagiri, Takashi Matsumoto, Shigeko Utsugi, Taiichi Ogawa, Hirokazu Handa, Hiroki Ishida, Masahiko Mori, Kanako Kawaura, Yasunari Ogihara, Makiko Chono, Ikuo Ashikawa, Hideho Miura

    NARO Achievement information 2011  2011.12 

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    Event date： 2011.12

    Language：Japanese  

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  • Identification of wheat germination suppressor gene MFT Invited

    Shingo Nakamura, Fumitaka Abe, Hiroyuki Kawahigashi, Kou Nakazono, Akemi Tagiri, Takashi Matsumoto, Shigeko Utsugi, Taiichi Ogawa, Hirokazu Handa, Hiroki Ishida, Masahiko Mori, Kanako Kawaura, Yasunari Ogihara, Makiko Chono, Ikuo Ashikawa, Hideho Miura

    NARO Achievement information 2011  2011.12 

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    Event date： 2011.12

    Language：Japanese  

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  • Sugar suppression of alfa-glucosidase activity in wheat embryos

    Rikiishi, K, Sugimoto, M, Maetani, M, Matsuura T, Utsugi, S, Maekawa, M, Noda, K

    Proc. 9th Int. Symp. Pre-Harvest Sprouting in Creals  2001 

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    Event date： 2001

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  • Complete sequence of wheat chloroplast DNA. IV. MITE like sequence found in wheat chloroplast DNA

    OGIHARA Y., ISONO K., KOJIMA T., ENDO A., MURAI R., MURAI K., HANAOKA M., SHIINA T., TERACHI T., UTSUGI S., MURATA M., MORI N., TAKUMI S., IKEO K., GOJOBORI T., MATSUOKA Y., OHNISHI Y., TAJIRI H., TSUNEWAKI K.

    2000.4 

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    Event date： 2000.4

    Language：Japanese  

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  • イネの斑入りアルビノ形質ヘテロ個体におけるアルビノ形質不分離

    前川雅彦, 力石和英, 松浦恭和, 宇都木繁子, 野田和彦

    育種学研究  2000 

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    Event date： 2000

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  • A System for Transient Assay of Transcriptional Factors Controlling Anthocyanin Biosynthesis at the Aleurone Layer of Mature Rice Seed

    MAEKAWA Masahiko, KAGEYAMA Keisuke, RIKIISHI Kazuhide, UTSUGI Shigeko, NODA Kazuhiko, SAKAMOTO Wataru

    Breeding Research  2000  Japanese Society of Breeding

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    Event date： 2000

    Language：Japanese  

    Various combinations of temperature and time for soaking seed were tested to establish a system for transient assay of transcriptional factors controlling anthocyanin biosynthesis to prove that the isolated gene is an inducible gene for anthocyanin expression at the aleurone layer of mature rice seed. The aleurone of mature seed of RIB-Pl with purple pericarp was sufficiently exposed under five combinations. Both Cl and B-Peru, transcriptional factors controlling anthocyanin biosynthesis of maize, were introduced by particle bombardment into the aleurone exposed under these five conditions. The highest expression of anthocyanin was obtained at the aleurone of the seeds soaked at 28°C for 4 hours. The effect of concentration of plasmids with Cl and B-Peru for introduction into the aleurone of A-58 mature seed with white pericarp soaked at 28°C for 4 hours was examined. Both Cl and B-Peru were found to give high expression of anthocyanin at the same concentration. Especially, 5 μg of Cl with 5 μg of B-Peru and 5 lig of Cl with 10 μg of B-Peru showed the highest expression. In conclusion, soaking of mature rice seed at 28°C for 4 hours and introduction of 5 lig of Cl with 51μg of B-Peru into the exposed aleurone was found to be a highly efficient system for transient assay of transcriptional factors controlling anthocyanin biosynthesis.

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    Other Link： https://agriknowledge.affrc.go.jp/RN/2010620894

  • Chinese spring wheat (Triticum aestivum L.) chloroplast genome: Complete sequence and contig clones

    Y Ogihara, K Isono, T Kojima, A Endo, M Hanaoka, T Shiina, T Terachi, S Utsugi, M Murata, N Mori, S Takumi, K Ikeo, T Gojobori, R Murai, K Murai, Y Matsuoka, Y Ohnishi, H Tajiri, K Tsunewaki

    PLANT MOLECULAR BIOLOGY REPORTER  2000  INT SOC PLANT MOLECULAR BIOLOGY

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    Event date： 2000

    Language：English  

    Libraries of plasmid clones covering the entire chloroplast (cp) genome of the common wheat, Triticum aestivum cv. Chinese Spring were constructed and assembled into contig-clones. From these, we obtained the complete nucleotide sequence of wheat chloroplast DNA - a 134,540 bp circular DNA (DDBJ accession no. AB042240) containing four species of ribosomal RNA, 30 genes for 20 species of transfer RNA, and 71 protein coding genes. Additionally, we detected five unidentified open reading frames conserved among grasses. Plasmid clones are available on request.

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  • Non-segregation of albino character in rice plants heterozygous for variegated albino

    MAEKAWA M., RIKIISHI K., MATSUURA T., UTSUGI S., NODA K.

    2000 

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    Event date： 2000

    Language：Japanese  

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  • Complete sequence of wheat chloroplast DNA

    OGIHARA Yasunari, ISONO Kazuhiro, KOJIMA Toshio, ENDO Akira, MURAI Rika, MURAI Koji, HANAOKA Mitsumasa, SHIINA Takashi, TERACHI Toru, UTSUGI Shigeko, MURATA Minoru, MORI Naoki, TAKUMI Shigeo, IKEO Kazuho, GOJOBORI Takashi, MATSUOKA Yoshihiro, OHNISHI Yukari, TAJIRI Hikaru, TSUNEWAKI Koichiro

    育種学研究  1998.12.1 

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    Event date： 1998.12.1

    Language：Japanese  

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  • Analysis of cDNA clone on flower vase peculiar gene expression in Arabidopsis.

    武智克彰, 宇都木繁子, 小倉豊, 坂本亘, 村田稔, 本吉総男

    日本遺伝学会大会プログラム・予稿集  1998 

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    Event date： 1998

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  • Analysis of CDNA clone on flower vase peculiar gene expression in Arabidopsis.

    武智克彰, 宇都木繁子, 小倉豊, 坂本亘, 村田稔, 本吉総男

    日本遺伝学会大会プログラム・予稿集  1997 

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    Event date： 1997

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  • Analysis of Arabidopsis Vsp gene.

    宇都木繁子, 村田稔, 坂本亘, 小倉豊, 本吉総男

    日本遺伝学会大会プログラム・予稿集  1997 

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    Event date： 1997

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  • Analysis of flowering organ specific genes in Arabidopsis.

    宇都木繁子, 坂本亘, 小倉豊, 村田稔, 本吉総男

    日本遺伝学会大会プログラム・予稿集  1995 

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    Event date： 1995

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  • 種子特異的に発現するオオムギTIP3;1による水輸送活性の調節

    第54回日本植物生理学会年会  2013 

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  • 原形質膜型アクアポリンPIP1とPIP2の共発現による活性抑制

    第54回日本植物生理学会年会  2013 

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  • Control of the water transport activity of a barley TIP3;1 specifically expressed in seeds

    2013 

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  • Molecular basis of water transport in Barley

    第53回日本植物生理学会年会  2012 

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  • A temperature-dependent seed dormancy (TMS) 2 gene acts in the regulation of germination in wheat

    第１２回国際穂発芽シンポジウム  2011 

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  • A temperature-dependent seed dormancy (TMS) 2 gene acts in the regulation of germination in wheat

    12th International Symposium on Pre-harvest Sprouting in Cereals  2011 

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  • オオムギMIPファミリーの解析

    日本育種学会第119回講演会  2011 

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  • Analysis of a MIP family in barley

    2011 

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  • Analysis of a bZIP type transcription factor, TaABF, which promotes seed dormancy in wheat

    12th International Symposium on Pre-harvest Sprouting in Cereals  2011 

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  • オオムギにおけるTIPファミリーの特徴と機能解明へのアプローチ

    第３回植物アクアポリン研究検討会  2011 

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  • Analysis of a bZIP type transcription factor, TaABF, which promotes seed dormancy in wheat

    第１２回国際穂発芽シンポジウム  2011 

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  • コムギにおける水チャネル遺伝子ファミリーの解析

    日本育種学会第115回講演会  2009 

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  • コムギ種子特異的な液胞型アクアポリン (TIP) の解析

    日本育種学会第116回講演会  2009 

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  • コムギ非休眠突然変異体の種子におけるマイクロアレイ解析

    日本育種学会第11３回講演会  2008 

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  • 六倍体コムギにおけるViviporous 1 (TaVp1) 遺伝子の発現とその特性

    日本育種学会第111回講演会  2007 

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  • コムギVIVIPAROUS1 (TaVP1) による種子の休眠と発芽の調節

    日本育種学会第110回講演会  2006 

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  • 種子休眠に関わるコムギbZIPタンパク質の解析

    日本育種学会第109回講演会・東京農業大学・2006年３月  2006 

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  • The analysis of a wheat ABI5 homologous gene.

    2005年8月 10th International congress of SABRAO  2005 

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  • 種子のアブシジン酸応答に関わるコムギbZIPタンパク質の解析

    日本分子生物学会年会  2005 

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  • Screening of hormone response mutants from GFP enhancer trap lines

    14th International Conference on Arabidopsis Research  2003 

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  • Wheat Rc gene for coleoptile color regulates the expression of DFR gene in developing coleoptile

    日本育種学会第101回講演会  2002 

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  • コムギ除胚半切種子で吸水によって誘導されるα-amylaseの特性

    日本育種学会第101回講演会  2002 

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  • Transactivation of late anthocyanin biosynthesis genes in developing wheat coleoptiles by maize C1 and B-Peru regulatory genes for anthocyanin synthesis

    日本育種学会第102回講演会  2002 

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  • α-アミラーゼ活性を示したコムギ除胚半切種子におけるデンプン分解酵素の解析

    日本育種学会第 100 回講演会  2001 

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  • コムギ糊粉層において行われるTmGAmybとTaVP1によるα-アミラーゼの発現調節

    日本育種学会第 100 回講演会  2001 

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  • Effect of wheat GAMyb and VP1 to α-Amylase expression

    9th International Symposium on Pre-harvest Sprouting in Cereals  2001 

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  • Suger suppression of α-Glucosidase activity in wheat embryos

    9th International Symposium on Pre-harvest Sprouting in Cereals.  2001 

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  • ジベレリンにより誘導されるコムギGAmyb遺伝子のクローニングと解析

    第 23 回日本分子生物学会  2000 

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  • コムギにおけるジベレリン関連遺伝子のクローニング及び発現解析

    日本育種学会第 97 回講演会  2000 

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  • コムギ幼穂で発現されるｃＤＮＡの大量解析

    第４回ムギ類分子生物学研究会  1999 

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Works

  • (財)ウエスコ(宇都木)

    2010
    -
    2011

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  • イネ科植物の水チャネル（液胞型アクアポリン）に関する分子生物学的研究

    2009
    -
    2010

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  • 岡山発これからの屋上ガーデン

    2009

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    Work type：Artistic work  

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  • 穀物種子の休眠形成と発芽調節機構に関する分子生物学的研究

    2008
    -
    2009

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Research Projects

  • 種子の休眠と発芽調節に関わる遺伝子群の分子生物学的解析

    Grant number：17780007  2005 - 2006

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    宇都木 繁子

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    Grant amount：\2800000 （ Direct expense: \2800000 ）

    本研究では、穂発芽が育種上の問題となっているコムギにおける種子休眠調節機構を明らかにすることを目的としている。特にコムギの倍数性に注目し、遺伝子の発現や機能調節について解析した。
    (1)コムギの種子休眠に関わる遺伝子の探索と解析
    コムギのABAシグナル伝達に関わるいくつかの遺伝子をクローニングして発現を解析した結果、TaVp1,TaABI5,TaPKABA1,Em遺伝子が種子特異的に発現し、その発現と種子休眠との間の関連性が示唆された。コムギ糊粉層におけるトランジェントアッセイにより、TaVp1とTaABI5はABA応答Em伝子プロモーターの転写を促進し、澱粉分解酵素α-アミラーゼ遺伝子プロモーターの転写を抑制する効果を示した。
    (2)コムギ非休眠突然変異体EH47-1におけるABAシグナル伝達
    開花後35日目のEH47-1の種子は、その親系統である北系1354と比較してABA感受性が著しく低下する。この種子胚では、TaVp1発現量が低下し、TaABI5発現量が増加傾向にあることから、TaVp1とTaABI5発現は異なって制御されており、その変異体の形質がTaABI5よりもTaVP1の影響を受けることが推定された。
    (3)六倍性コムギにおける遺伝子発現の調節
    TaVp1は、他の植物のオーソログと同様に種子発達から登熟期で主に発現するが、それぞれの同祖遺伝子の発現パターンは全く異なっており、機能が分化していることが推測された。また、TaVp1は、転写後のスプライシングにより少なくとも6タイプの転写産物を生じ、そのうち4タイプは機能ドメインの欠失やフレームシフトによる終止コドン出現のため、正常な機能を失っていると推測された。このミススプライシングのほとんどは、同祖遺伝子間のいくつかの塩基配列の違いにより、TaVp1-A1から由来するものであった。TaABI5もまた、転写後に3'領域で選択的にスプライシングされ、少なくとも2種類の転写産物(TaABI5-IとTaABI5-II)を生じ、TaABI5-IIはTaABI5-Iと比較して転写活性化能力が低いことが示された。休眠/発芽調節との関連は未だ未解明であるが、少なくとも六倍性コムギの種子におけるABA関連遺伝子の発現調節には、転写レベルの調節に加えて転写後の調節が大きく関与しているものと推測される。

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  • コムギの種子休眠性に関わる遺伝子群の分子生物学的解析

    Grant number：14760004  2002

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    宇都木 繁子

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    Grant amount：\1800000 （ Direct expense: \1800000 ）

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  • Plant Morphology （2024academic year） Third semester  - 水1,水2

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