Updated on 2025/09/30

写真a

 
TAKETA Shin
 
Organization
Scheduled update Professor
Position
Professor
External link

Degree

  • 博士（農学） ( 1992.11   京都大学 )

Research Interests

  • Plant genetics and breeding

  • 植物遺伝育種学

  • 植物生理学

Research Areas

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

Professional Memberships

 

Papers

  • SHORT AND CROOKED AWN, encoding an epigenetic regulator EMF1, promotes barley awn development Reviewed

    Koki Nakamura, Yuichi Kikuchi, Mizuho Shiraga, Toshihisa Kotake, Kiwamu Hyodo, Shin Taketa, Yoko Ikeda

    Plant and Cell Physiology (accepted)   2024.12

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  • Genomic traces of Japanese malting barley breeding in two modern high-quality cultivars, ‘Sukai Golden’ and ‘Sachiho Golden’ Reviewed

    Shin Taketa, June-Sik Kim, Hidekazu Takahashi, Shunsuke Yajima, Yuichi Koshiishi, Toshinori Sotome, Tsuneo Kato, Keiichi Mochida

    Breeding Science   73 ( 5 )   2023.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Breeding  

    DOI： 10.1270/jsbbs.23031

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  • Molecular identification of a laccase that catalyzes the oxidative coupling of a hydroxycinnamic acid amide for hordatine biosynthesis in barley Reviewed

    Naoki Ube, Atsushi Ishihara, Yukinori Yabuta, Shin Taketa, Yasuo Kato, Taiji Nomura

    The Plant Journal   2023.5

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1111/tpj.16278

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  • A bifurcated palea mutant infers functional differentiation of WOX3 genes in flower and leaf morphogenesis of barley Reviewed

    Takanori Yoshikawa, Hiroshi Hisano, Ken-Ichiro Hibara, Jilu Nie, Yuki Tanaka, Jun-Ichi Itoh, Shin Taketa

    AoB PLANTS   14 ( 3 )   2022.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    Barley (Hordeum vulgare) is the fourth most highly produced cereal in the world after wheat, rice and maize and is mainly utilized as malts and for animal feed. Barley, a model crop of the tribe Triticeae, is important in comparative analyses of Poaceae. However, molecular understanding about the developmental processes is limited in barley. Our previous work characterized one of two WUSCHEL-RELATED HOMEOBOX 3 (WOX3) genes present in the barley genome: NARROW LEAFED DWARF1 (NLD1). We demonstrated that NLD1 plays a pivotal role in the development of lateral organs. In the present study, we describe a bifurcated palea (bip) mutant of barley focusing on flower and leaf phenotypes. The palea in the bip mutant was split into two and develop towards inside the lemma surrounding the carpels and anthers. The bip mutant is devoid of lodicules, which develop in a pair at the base of the stamen within the lemma in normal barley. bip also exhibited malformations in leaves, such as narrow leaf due to underdeveloped leaf-blade width, and reduced trichome density. Map-based cloning and expression analysis indicated that BIP is identical to another barley WOX3 gene, named HvWOX3. The bip nld1 double mutant presented a more severe reduction in leaf-blade width and number of trichomes. By comparing the phenotypes and gene expression patterns of various WOX3 mutants, we concluded that leaf bilateral outgrowth and trichome development are promoted by both NLD1 and HvWOX3, but that HvWOX3 serves unique and pivotal functions in barley development that differ from those of NLD1.

    DOI： 10.1093/aobpla/plac019

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    Other Link： https://academic.oup.com/aobpla/article-pdf/14/3/plac019/43934060/plac019.pdf

  • Regulation of the plastochron by three many-noded dwarf genes in barley Reviewed

    Ken-Ichiro Hibara, Masayuki Miya, Sean Akira Benvenuto, Naoko Hibara-Matsuo, Manaki Mimura, Takanori Yoshikawa, Masaharu Suzuki, Makoto Kusaba, Shin Taketa, Jun-ichi Itoh

    PLOS GENETICS   17 ( 5 )   2021.5

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    DOI： 10.1371/journal.pgen.1009292

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  • Identification of methoxylchalcones produced in response to CuCl2 treatment and pathogen infection in barley Reviewed

    Naoki Ube, Yuhka Katsuyama, Keisuke Kariya, Shin-ichi Tebayashi, Masayuki Sue, Takuji Tohnooka, Kotomi Ueno, Shin Taketa, Atsushi Ishihara

    PHYTOCHEMISTRY   184   2021.4

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    DOI： 10.1016/j.phytochem.2020.112650

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  • Mutations in a Golden2-Like Gene Cause Reduced Seed Weight in Barley albino lemma 1 Mutants Reviewed

    Shin Taketa, Momoko Hattori, Tsuneaki Takami, Eiko Himi, Wataru Sakamoto

    PLANT AND CELL PHYSIOLOGY   62 ( 3 )   447 - 457   2021.3

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    DOI： 10.1093/pcp/pcab001

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  • Phylogenetic analysis of the Si7PPO gene in foxtail millet, Setaria italica, provides further evidence for multiple origins of the negative phenol color reaction phenotype. Reviewed

    Kenji Fukunaga, Meili Zakiyah Nur, Takahiko Inoue, Shin Taketa, Katsuyuki Ichitani

    Genes & genetic systems   95 ( 4 )   191 - 199   2020.10

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    To elucidate the diversity and evolution of the Si7PPO gene that controls phenol color reaction (Phr) in foxtail millet, Setaria italica, we analyzed sequence polymorphisms of the Si7PPO gene in 39 accessions consisting of foxtail millet landraces (32 accessions) and their wild ancestor ssp. viridis (seven accessions) collected from various regions in Europe and Asia. The accessions included wild type (positive Phr) and three different types of loss-of-function phenotype (negative Phr), "stop codon type", "TE1-insertion type" and "6-bp duplication type", found in our previous study. We constructed a phylogenetic tree of the gene and found that accessions with positive Phr showed higher genetic diversity at the nucleotide sequence level. We also found that the three different loss-of-function types formed different clusters, suggesting that landraces with negative Phr have multiple origins from three different lineages including both landrace and ssp. viridis accessions with positive Phr.

    DOI： 10.1266/ggs.20-00011

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  • Biosynthesis of Phenylamide Phytoalexins in Pathogen-Infected Barley Reviewed

    Naoki Ube, Yukinori Yabuta, Takuji Tohnooka, Kotomi Ueno, Shin Taketa, Atsushi Ishihara

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   20 ( 22 )   2019.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/ijms20225541

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  • Identification of phenylamide phytoalexins and characterization of inducible phenylamide metabolism in wheat Reviewed

    Naoki Ube, Daiyu Harada, Yuhka Katsuyama, Kumiko Osaki-Oka, Takuji Tonooka, Kotomi Ueno, Shin Taketa, Atsushi Ishihara

    PHYTOCHEMISTRY   167   2019.11

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    DOI： 10.1016/j.phytochem.2019.112098

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  • Genebank genomics highlights the diversity of a global barley collection Reviewed

    Sara G. Milner, Matthias Jost, Shin Taketa, Elena Rey Mazon, Axel Himmelbach, Markus Oppermann, Stephan Weise, Helmut Knuepffer, Martin Basterrechea, Patrick Koenig, Danuta Schueler, Rajiv Sharma, Raj K. Pasam, Twan Rutten, Ganggang Guo, Dongdong Xu, Jing Zhang, Gerhard Herren, Thomas Mueller, Simon G. Krattinger, Beat Keller, Yong Jiang, Maria Y. Gonzalez, Yusheng Zhao, Antje Habekuss, Sandra Faerber, Frank Ordon, Matthias Lange, Andreas Boerner, Andreas Graner, Jochen C. Reif, Uwe Scholz, Martin Mascher, Nils Stein

    NATURE GENETICS   51 ( 2 )   319 - +   2019.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41588-018-0266-x

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  • Evolutionary changes in defensive specialized metabolism in the genus Hordeum Reviewed

    Naoki Ube, Miho Nishizaka, Tsuyoshi Ichiyanagi, Kotomi Ueno, Shin Taketa, Atsushi Ishihara

    PHYTOCHEMISTRY   141   1 - 10   2017.9

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    DOI： 10.1016/j.phytochem.2017.05.004

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  • Distribution of the tryptophan pathway-derived defensive secondary metabolites gramine and benzoxazinones in Poaceae Reviewed

    Yu Kokubo, Miho Nishizaka, Naoki Ube, Yukinori Yabuta, Shin-ichi Tebayashi, Kotomi Ueno, Shin Taketa, Atsushi Ishihara

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   81 ( 3 )   431 - 440   2017.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/09168451.2016.1256758

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  • Barley NARROW LEAFED DWARF1 encoding a WUSCHEL-RELATED HOMEOBOX 3 (WOX3) regulates the marginal development of lateral organs Reviewed

    Takanori Yoshikawa, Shin-Ya Tanaka, Yuuki Masumoto, Naoya Nobori, Hiroto Ishii, Ken-Ichiro Hibara, Jun-Ichi Itoh, Takatoshi Tanisaka, Shin Taketa

    BREEDING SCIENCE   66 ( 3 )   416 - 424   2016.6

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    DOI： 10.1270/jsbbs.16019

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  • Isolation of candidate genes for the barley Ant1 and wheat Rc genes controlling anthocyanin pigmentation in different vegetative tissues Reviewed

    Eiko Himi, Shin Taketa

    MOLECULAR GENETICS AND GENOMICS   290 ( 4 )   1287 - 1298   2015.8

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    DOI： 10.1007/s00438-015-0991-0

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  • Multiple origins of the phenol reaction negative phenotype in foxtail millet, Setaria italica (L.) P. Beauv., were caused by independent loss-of-function mutations of the polyphenol oxidase (Si7PPO) gene during domestication Reviewed

    Takahiko Inoue, Takahisa Yuo, Takeshi Ohta, Eriko Hitomi, Katsuyuki Ichitani, Makoto Kawase, Shin Taketa, Kenji Fukunaga

    MOLECULAR GENETICS AND GENOMICS   290 ( 4 )   1563 - 1574   2015.8

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    DOI： 10.1007/s00438-015-1022-x

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  • Real-time PCR-mediated diagnosis of hemizygosity at the Tamyb10-D1 locus controlling grain color in wheat Reviewed

    Himi Eiko, Maekawa Masahiko, Matsuura Takakazu, Taketa Shin

    MOLECULAR BREEDING   35 ( 3 )   2015.3

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  • Barley Ant17, encoding flavanone 3-hydroxylase (F3H), is a promising target locus for attaining anthocyanin/proanthocyanidin-free plants without pleiotropic reduction of grain dormancy. Reviewed

    Himi E, Taketa S

    Genome   58 ( 1 )   43 - 53   2015.1

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  • Ant28 gene for proanthocyanidin synthesis encoding the R2R3 MYB domain protein (Hvmyb10) highly affects grain dormancy in barley Reviewed

    Himi Eiko, Yamashita Yuko, Haruyama Naoto, Yanagisawa Takashi, Maekawa Masahiko, Taketa Shin

    EUPHYTICA   188 ( 1 )   141 - 151   2012.11

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    DOI： 10.1007/s10681-011-0552-5

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  • A SHORT INTERNODES (SHI) family transcription factor gene regulates awn elongation and pistil morphology in barley. Reviewed International journal

    Takahisa Yuo, Yuko Yamashita, Hiroyuki Kanamori, Takashi Matsumoto, Udda Lundqvist, Kazuhiro Sato, Masahiko Ichii, Stephen A Jobling, Shin Taketa

    Journal of experimental botany   63 ( 14 )   5223 - 32   2012.9

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    The awn, an apical extension from the lemma of the spikelet, plays important roles in seed dispersal, burial, and photosynthesis. Barley typically has long awns, but short-awn variants exist. The short awn 2 (lks2) gene, which produces awns about 50% shorter than normal, is a natural variant that is restricted to Eastern Asia. Positional cloning revealed that Lks2 encodes a SHI-family transcription factor. Allelism tests showed that lks2 is allelic to unbranched style 4 (ubs4) and breviaristatum-d (ari-d), for which the phenotypes are very short awn and sparse stigma hairs. The gene identity was validated by 25 mutant alleles with lesions in the Lks2 gene. Of these, 17 affected either or both conserved regions: the zinc-binding RING-finger motif and the IGGH domain. Lks2 is highly expressed in awns and pistils. Histological observations of longitudinal awn sections showed that the lks2 short-awn phenotype resulted from reduced cell number. Natural variants of lks2 were classified into three types, but all shared a single-nucleotide polymorphism (SNP) that causes a proline-to-leucine change at position 245 in the IGGH domain. All three lks2 natural variants were regarded as weak alleles because their awn and pistil phenotypes are mild compared with those of the 25 mutant alleles. Natural variants of lks2 found in the east of China and the Himalayas had considerably different sequences in the regions flanking the critical SNP, suggesting independent origins. The available results suggest that the lks2 allele might have a selective advantage in the adaptation of barley to high-precipitation areas of Eastern Asia.

    DOI： 10.1093/jxb/ers182

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  • Functional characterization of barley betaglucanless mutants demonstrates a unique role for CslF6 in (1,3;1,4)-beta-D-glucan biosynthesis Reviewed

    Shin Taketa, Takahisa Yuo, Takuji Tonooka, Yoichi Tsumuraya, Yoshiaki Inagaki, Naoto Haruyama, Oscar Larroque, Stephen A. Jobling

    JOURNAL OF EXPERIMENTAL BOTANY   63 ( 1 )   381 - 392   2012.1

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    DOI： 10.1093/jxb/err285

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  • Root hairless 2 (rth2) mutant represents a loss-of-function allele of the cellulose synthase-like gene OsCSLD1 in rice (Oryza sativa L.) Reviewed

    Yuo Takahisa, Shiotani Kenji, Shitsukawa Naoki, Miyao Akio, Hirochika Hirohiko, Ichii Masahiko, Taketa Shin

    BREEDING SCIENCE   61 ( 3 )   225 - 233   2011.9

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  • Expression and functional analysis of the barley Nud gene using transgenic rice Reviewed

    Katsuyuki Kakeda, Norimitsu Ishihara, Yohei Izumi, Kazuhiro Sato, Shin Taketa

    BREEDING SCIENCE   61 ( 1 )   35 - 42   2011.3

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    DOI： 10.1270/jsbbs.61.35

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  • Molecular mapping of the short awn 2 (lks2) and dense spike 1 (dsp1) genes on barley chromosome 7H Reviewed

    Shin Taketa, Takahisa Yuo, Yukie Sakurai, Shoko Miyake, Masahiko Ichii

    BREEDING SCIENCE   61 ( 1 )   80 - 85   2011.3

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    DOI： 10.1270/jsbbs.61.80

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  • Duplicate polyphenol oxidase genes on barley chromosome 2H and their functional differentiation in the phenol reaction of spikes and grains Reviewed

    Shin Taketa, Kanako Matsuki, Satoko Amano, Daisuke Saisho, Eiko Himi, Naoki Shitsukawa, Takahisa Yuo, Kazuhiko Noda, Kazuyoshi Takeda

    JOURNAL OF EXPERIMENTAL BOTANY   61 ( 14 )   3983 - 3993   2010.9

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    DOI： 10.1093/jxb/erq211

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  • Molecular phylogeny of the genus Hordeum using thioredoxin-like gene sequences

    Katsuyuki Kakeda, Shin Taketa, Takao Komatsuda

    BREEDING SCIENCE   59 ( 5 )   595 - 601   2009.12

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    DOI： 10.1270/jsbbs.59.595

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  • Analysis of the rice SHORT-ROOT5 gene revealed functional diversification of plant neutral/alkaline invertase family Reviewed

    Shan-Guo Yao, Rumi Kodama, Hua Wang, Masahiko Ichii, Shin Taketa, Hitoshi Yoshida

    PLANT SCIENCE   176 ( 5 )   627 - 634   2009.5

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    DOI： 10.1016/j.plantsci.2009.02.002

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  • Molecular cloning of a root hairless gene rth1 in rice Reviewed

    Yuo Takahisa, Toyota Masanori, Ichii Masahiko, Taketa Shin

    Breeding science   59 ( 1 )   13-20 - 20   2009.3

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    Authorship：Last author,　Corresponding author   Language：English   Publisher：Japanese Society of Breeding  

    Root hairs, projections from the epidermal cells of the roots, are contributing to water and nutrient uptake and anchorage to the soil. To better understand genetic control of root hair formation in rice, we analyzed root hairless 1 (rth1) mutant that was induced by NaN_3 treatment. SEM observation showed that in rth1 plants, root hair elongation was abolished after the formation of bulge. High-resolution mapping using 2,088 segregants revealed three predicted genes in a 38-kb candidate interval on chromosome 7. Sequences comparison of the three genes between wild-type Oochikara and rth1 detected a nucleotide substitution only in apyrase (OsAPY). This nucleotide substitution (G→A) lies in the junction between the third intron and the forth exon, and results in the splicing anomaly to the rth1 cDNA sequence. Transgenic plants with introduced OsAPY allele restored normal root hairs and plant growth, showing a complementation of rth1 phenotype. We concluded that the root hairless phenotype of rth1 is caused by a mutation of OsAPY. OsAPY appears to be an important gene for root hair elongation and plant growth in rice.

    DOI： 10.1270/jsbbs.59.13

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    Other Link： http://shark.lib.kagawa-u.ac.jp/kuir/metadata/3367

  • A novel mutant gene for (1-3, 1-4)-β-D-glucanless grain on barley (Hordeum vulgare L.) chromosome 7H

    Tonooka Takuji, Aoki Emiko, Yoshioka Toji, Taketa Shin

    Breeding science   59 ( 1 )   47 - 54   2009.3

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    (1-3, 1-4)-β-D-glucan contained in barley (Hordeum vulgare L.) grains is a main component of endosperm cell walls and constitutes the cell wall matrix with arabinoxylan. A monofactorial recessive mutant gene for (1-3, 1-4)-β-D-glucanless grain was found and the new gene was designated as bgl (=(1-3, 1-4)-beta-D-glucanless grain). A linkage was found between the bgl gene and the naked caryopsis (nud) gene, and it was mapped to the centromeric region of chromosome 7H. Phenotypes in bgl cosegregated with the polymorphisms in HvCslF6, a member of cellulose synthase-like HvCslF gene family, indicating that bgl was caused by a mutation in the HvCslF6 locus. In order to clarify the characteristics of (1-3, 1-4)-β-D-glucanless barley grains, a near-isogenic line (NIL) was developed by backcrossing the Japanese two-rowed cultivar 'Nishinohoshi' as a recurrent parent. The NIL with bgl completely lacked (1-3, 1-4)-β-D-glucan in both the endosperm and aleurone layer cell walls. Microscopic analysis revealed that the NIL had thin endosperm cell walls. It also showed a softer grain texture and many more broken grains during the pearling process than the recurrent parent. The soft and friable grain texture of the NIL was probably caused by the thin endosperm cell walls.

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  • Fine Mapping of Short Rachilla Hair Gene (srh) in Barley and an Association Study using Flanking Molecular Markers and World Germplasms

    JAVAID Asif, IKEDA Shigeru, KATAOKA Ikuo, TAKETA Shin

    Asian Journal of Plant Sciences   8 ( 6 )   400 - 408   2009

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    DOI： 10.3923/ajps.2009.400.408

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  • Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway. Reviewed International journal

    Shin Taketa, Satoko Amano, Yasuhiro Tsujino, Tomohiko Sato, Daisuke Saisho, Katsuyuki Kakeda, Mika Nomura, Toshisada Suzuki, Takashi Matsumoto, Kazuhiro Sato, Hiroyuki Kanamori, Shinji Kawasaki, Kazuyoshi Takeda

    Proceedings of the National Academy of Sciences of the United States of America   105 ( 10 )   4062 - 7   2008.3

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    In contrast to other cereals, typical barley cultivars have caryopses with adhering hulls at maturity, known as covered (hulled) barley. However, a few barley cultivars are a free-threshing variant called naked (hulless) barley. The covered/naked caryopsis is controlled by a single locus (nud) on chromosome arm 7HL. On the basis of positional cloning, we concluded that an ethylene response factor (ERF) family transcription factor gene controls the covered/naked caryopsis phenotype. This conclusion was validated by (i) fixation of the 17-kb deletion harboring the ERF gene among all 100 naked cultivars studied; (ii) two x-ray-induced nud alleles with a DNA lesion at a different site, each affecting the putative functional motif; and (iii) gene expression strictly localized to the testa. Available results indicate the monophyletic origin of naked barley. The Nud gene has homology to the Arabidopsis WIN1/SHN1 transcription factor gene, whose deduced function is control of a lipid biosynthesis pathway. Staining with a lipophilic dye (Sudan black B) detected a lipid layer on the pericarp epidermis only in covered barley. We infer that, in covered barley, the contact of the caryopsis surface, overlaid with lipids to the inner side of the hull, generates organ adhesion.

    DOI： 10.1073/pnas.0711034105

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  • Rice blast disease and susceptibility to pests in a silicon uptake-deficient mutant lsil of rice Reviewed

    Y. Nakata, M. Uena, J. Kihara, M. Ichii, S. Taketa, S. Arase

    CROP PROTECTION   27 ( 3-5 )   865 - 868   2008.3

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    DOI： 10.1016/j.cropro.2007.08.016

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  • Saturated humidity accelerates lateral root development in rice (Orysa sativa L.) seedlings by increasing phloem- based auxin transport Reviewed

    Tory Chhun, Yuichi Uno, Shin Taketa, Tetsushi Azuma, Masahiko Ichii, Takashi Okamoto, Seiji Tsurumi

    Journal of Experimental Botany   58 ( 7 )   1695 - 1704   2007

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press  

    DOI： 10.1093/jxb/erm026

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  • High-resolution mapping of the nud locus controlling the naked caryopsis in barley Reviewed

    S. Taketa, T. Awayama, S. Amano, Y. Sakurai, M. Ichii

    PLANT BREEDING   125 ( 4 )   337 - 342   2006.8

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  • Isolation of a novel lateral-rootless mutant in rice (Oryza sativa L.) with reduced sensitivity to auxin Reviewed

    Wang, H., Taketa, S., Miyao, A., Hirochika, H., Ichii, M.

    Plant Science   170 ( 1 )   70 - 77   2006

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    DOI： 10.1016/j.plantsci.2005.08.005

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  • Physiological Characteristics of Rice Cultivar Oochikara and Its Short-root Near Isogenic Line IL-srt1 Reviewed

    ZHAO Rengui, LIU Jian, SHIOTSU Fumitaka, TOYOTA Masanori, KUSUTANI Akihito, TAKETA Shin, ICHII Masahiko

    Japanese Journal of Crop Science   75 ( 2 )   148 - 152   2006

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    Language：Japanese   Publisher：The Crop Science Society of Japan  

    The shoot and root characters, the bleeding rate and the photosynthetic rate in flag leaves of rice cultivar Oochikara were compared with its short-root near isogenic line IL-srt1 at the heading stage. The bleeding rate per plant in IL-srt1 was only 56% of that in Oochikara. The total root length and total root weight in IL-srt1 were 30% and 35% of those in Oochikara, respectively. On the other hand, the bleeding rate per total root length and that per total root weight in IL-srt1 were 188% and 163% of those in Oochikara, respectively, showing a significant difference between the two lines. Thus, although the root mass was larger in Oochikara than in IL-srt1, the bleeding rate per unit root mass was higher in IL-srt1 than in Oochikara. These results suggest that the physiological activities of total root in IL-srt1 were lower than that in Oochikara, due to the smaller root mass, and that IL-srt1 had higher physiological activities per unit root mass than Oochikara. There was no significant difference in photosynthetic rate between the two lines, suggesting that the short-root gene srt1 did not affect the photosynthesis.

    DOI： 10.1626/jcs.75.148

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    Other Link： http://agriknowledge.affrc.go.jp/RN/2010723569

  • Involvement of ARM2 in the uptake of indole-3-butyric acid in rice (Oryza sativa L.) roots Reviewed

    T Chhun, S Taketa, M Ichii, S Tsurumi

    PLANT AND CELL PHYSIOLOGY   46 ( 7 )   1161 - 1164   2005.7

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    DOI： 10.1093/pcp/pci117

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  • Ancestry of American polyploid Hordeum species with the I genome inferred from 5S and 18S-25S rDNA Reviewed

    S Taketa, H Ando, K Takeda, M Ichii, R Von Bothmer

    ANNALS OF BOTANY   96 ( 1 )   23 - 33   2005.7

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    DOI： 10.1093/aob/mci147

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  • Defects in root development and gravity response in the aem1 mutant of rice are associated with reduced auxin efflux

    BR Debi, T Chhun, S Taketa, S Tsurumi, K Xia, A Miyao, H Hirochika, M Ichii

    JOURNAL OF PLANT PHYSIOLOGY   162 ( 6 )   678 - 685   2005.6

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    DOI： 10.1016/j.jplph.2004.09.012

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  • Cytokinin inhibits lateral root initiation but stimulates lateral root elongation in rice (Oryza sativa)

    BR Debi, S Taketa, M Ichii

    JOURNAL OF PLANT PHYSIOLOGY   162 ( 5 )   507 - 515   2005.5

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    DOI： 10.1016/j.jplph.2004.08.007

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  • Molecular cytogenetic identification of nullisomy 5B induced homoeologous recombination between wheat chromosome 5D and barley chromosome 5H

    S Taketa, T Awayama, M Ichii, M Sunakawa, T Kawahara, K Murai

    GENOME   48 ( 1 )   115 - 124   2005.2

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    DOI： 10.1139/G04-096

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  • Molecular Genetic Analysis of a Tos17-Tagged Mutant Line Related to Root Morphology in Rice

    Iwao-Mushika Junko, Yuo Takahisa, Taketa Shin, Miyao Akio, Hirochika Hirohiko, Ichii Masahiko

    Breeding Research   7 ( 4 )   171 - 178   2005

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    Although plant roots are one of the most important organs for crop cultivation, root studies lag behind due to various difficulties. The objective of the present study was to isolate genes related to root morphology using 'mutant panel', rice retrotransposon Tos17-insertion mutant lines. First, we grew 5,568 R1 lines regenerated from tissue culture of Oryza sativa L. cv. Nipponbare in water culture, and screened the lines which segregated root mutants at the seedling stage. Then, 56 root mutant lines that were confirmed by the progeny test, were analyzed by genomic Southern hybridization using Tos17 as a probe. As a result of linkage analysis, we obtained one Tos17-tagged mutant line, NC6949. The phenotype of this mutant line was as follows: dwarf, narrow leaves and reduced numbers of crown roots and lateral roots. In this line, Tos17 was inserted in the third exon of the putative alliinase gene on the short arm of chromosome 1 and the presence of this insertion cosegregated with the mutant phenotype. We designated the gene tagged by Tos17, as OsAll1 (Oryza sativa alliinase 1). In a complementary experiment in which a 6kb genomic fragment containing alliinase/ORFs and its putative promoter region was introduced into the osall1 mutant, the root morphology in the transgenic plant was rescued. Based on these observations, it was concluded that the abnormal root morphology of the osall1 mutant obtained in this study was caused by knock out of the OsAll1 gene. In the rice genome, at least 3 other alliinase-like gene sequences are present and form a multigene family, but their expression patterns were different, suggesting that OsAll1 was functionally differentiated from the others.<br>

    DOI： 10.1270/jsbbr.07.171

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  • Involvement of ARM2 in the uptake of indole-3-butyric acid in rice (Oryza sativa L.) roots

    T Chhun, S Taketa, M Ichii, S Tsurumi

    PLANT AND CELL PHYSIOLOGY   46   S209 - S209   2005

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  • Root Morphology and Interrelationship between Shoot and Root Growth of a Short-root Near Isogenic Line IL-srt1 Derived from Rice Cultivar Oochikara

    ZHAO Rengui, LIU Jian, SHIOTSU Fumitaka, TOYOTA Masanori, KUSUTANI Akihito, TAKETA Shin, ICHII Masahiko

    Root Research   14 ( 4 )   157 - 164   2005

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    The growth and morphology of IL-srt1, a short-root near isogenic line derived from rice cultivar Oochikara, were characterized by paddy field and pot experiments, with particular focus on the interrelationship between shoot and root growth. The shoot dry weight, stem number, root length and root dry weight per hill of IL-srt1 were lower than those of Oochikara, but the number of crown roots per hill was the same for two cultivars (line). The root weight/root length ratio of IL-srt1 was higher than that of Oochikara, which was attributed to the difference of the mean root diameter, not to the specific gravity of roots. Root system surveys by monolith method revealed that IL-srt1 had smaller root length density at everywhere in soil, and narrow rooting zone. The number of stem per hill was responsible for the difference of shoot dry weight per hill, and there was no difference in shoot dry weight per stem. It was assumed that larger number of crown roots and higher percentage of young crown roots of IL-srt1 as compared to Oochikara might compensate for shorter root length per stem, and attained the same water and nutrient supply per stem. It was also assumed that the rooting zone would closely relate to the emergence and survival of tillers through the ability of water and nutrient supply.

    DOI： 10.3117/rootres.14.157

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  • The short-root mutation srt5 defines a sugar-mediated root growth in rice (Oryza sativa L.) Reviewed

    SG Yao, J Mushika, S Taketa, M Ichii

    PLANT SCIENCE   167 ( 1 )   49 - 54   2004.7

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    DOI： 10.1016/j.plantsci.2004.02.025

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  • Different behaviour of indole-3-acetic acid and indole-3-butyric acid in stimulating lateral root development in rice (Oryza sativa L.) Reviewed

    T Chhun, S Taketa, S Tsurumi, M Ichii

    PLANT GROWTH REGULATION   43 ( 2 )   135 - 143   2004.6

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  • Monophyletic origin of naked barley inferred from molecular analyses of a marker closely linked to the naked caryopsis gene (nud) Reviewed

    S Taketa, S Kikuchi, T Awayama, S Yamamoto, M Ichii, S Kawasaki

    THEORETICAL AND APPLIED GENETICS   108 ( 7 )   1236 - 1242   2004.5

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    DOI： 10.1007/s00122-003-1560-1

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  • Interaction between two auxin-resistant mutants and their effects on lateral root formation in rice (Oryza sativa L.) Reviewed

    T Chhun, S Taketa, S Tsurumi, M Ichii

    JOURNAL OF EXPERIMENTAL BOTANY   54 ( 393 )   2701 - 2708   2003.12

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    DOI： 10.1093/jxb/erg306

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  • Efficient fine mapping of the naked caryopsis gene (nud) by HEGS (High Efficiency Genome Scanning)/AFLP in barley Reviewed

    S Kikuchi, S Taketa, M Ichii, S Kawasaki

    THEORETICAL AND APPLIED GENETICS   108 ( 1 )   73 - 78   2003.12

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    DOI： 10.1007/s00122-003-1413-y

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  • Lateral root formation in rice (Oryza Sativa L.): differential effects of indole-3-acetic acid and indole-3-butyric acid Reviewed

    SC Wang, S Taketa, M Ichii, LL Xu, K Xia, Zhou, X

    PLANT GROWTH REGULATION   41 ( 1 )   41 - 47   2003.9

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  • Morphological and physiological characteristics of a root-hairless mutant in rice (Oryza sativa L.) Reviewed

    N Suzuki, S Taketa, M Ichii

    PLANT AND SOIL   255 ( 1 )   9 - 17   2003.8

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  • Isolation and characterization of an abscisic acid-in sensitive mutation that affects specifically primary root elongation in rice (Oryza sativa L.) Reviewed

    SG Yao, S Taketa, M Ichii

    PLANT SCIENCE   164 ( 6 )   971 - 978   2003.6

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    DOI： 10.1016/S0168-9452(03)00081-5

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  • The effects of auxin on lateral root initiation and root gravitropism in a lateral rootless mutant Lrt1 of rice (Oryza sativa L.) Reviewed

    T Chhun, S Taketa, S Tsurumi, M Ichii

    PLANT GROWTH REGULATION   39 ( 2 )   161 - 170   2003.2

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  • Characterization of CMS and maintainer lines in indica rice (Oryza sativa L.) based on RAPD marker analysis Reviewed

    M Ichii, DL Hong, Y Ohara, CM Zhao, S Taketa

    EUPHYTICA   129 ( 2 )   249 - 252   2003

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  • Isolation and characterization of a short lateral root mutant in rice (Oryza sativa L.) Reviewed

    Debi, B.R., Mushika, J., Taketa, S., Miyao, A., Hirochika, H., Ichii, M.

    Plant Science   165 ( 4 )   2003

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    DOI： 10.1016/S0168-9452(03)00293-0

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  • Lateral root formation in rice (Oryza sativa): promotion effect of jasmonic acid Reviewed

    SC Wang, M Ichii, S Taketa, LL Xu, K Xia, Zhou, X

    JOURNAL OF PLANT PHYSIOLOGY   159 ( 8 )   827 - 832   2002.8

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  • Molecular and physical mapping of a barley gene on chromosome arm 1HL that causes sterility in hybrids with wheat Reviewed

    S Taketa, M Choda, R Ohashi, M Ichii, K Takeda

    GENOME   45 ( 4 )   617 - 625   2002.8

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    DOI： 10.1139/G02-024

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  • A novel short-root gene that affects specifically early root development in rice (Oryza sativa L.) Reviewed

    SG Yao, S Taketa, M Ichii

    PLANT SCIENCE   163 ( 2 )   207 - 215   2002.8

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  • Effect of jasmonic acid on lateral root formation in rice seedling Reviewed

    SC Wang, M Ichii, S Taketa, LL Xu, K Xia, Zhou, X

    ACTA BOTANICA SINICA   44 ( 4 )   502 - 504   2002.4

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  • A DNA marker closely linked to the vrs1 locus (row-type gene) indicates multiple origins of six-rowed cultivated barley (Hordeum vulgare L.) Reviewed

    K Tanno, S Taketa, K Takeda, T Komatsuda

    THEORETICAL AND APPLIED GENETICS   104 ( 1 )   54 - 60   2002.1

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  • Differential effects of cultivated and wild barley 5H chromosomes on heading characters in wheat-barley chromosome addition lines Reviewed

    T Kawahara, S Taketa, K Murai

    HEREDITAS   136 ( 3 )   195 - 200   2002

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  • Chromosome arm location of the gene controlling leaf pubescence of a Chinese local wheat cultivar 'Hong-mang-mai' Reviewed

    S Taketa, CL Chang, M Ishii, K Takeda

    EUPHYTICA   125 ( 2 )   141 - 147   2002

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  • Chromosomes responsible for sensitivity of embryo to abscisic acid and dormancy in wheat

    NODA K.

    Euphytica   123   203 - 209   2002

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  • Production and characterization of a complete set of wheat-wild barley (Hordeum vulgare ssp spontaneum) chromosome addition lines Reviewed

    S Taketa, K Takeda

    BREEDING SCIENCE   51 ( 3 )   199 - 206   2001.9

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  • Physical locations of 5S and 18S-25S rDNA in Asian and American diploid Hordeum species with the I genome Reviewed

    S Taketa, H Ando, K Takeda, R Von Bothmer

    HEREDITY   86   522 - 530   2001.5

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  • The influence of a spring habit gene, Vrn-D1, on heading time in wheat Reviewed

    H Kato, S Taketa, T Ban, N Iriki, K Murai

    PLANT BREEDING   120 ( 2 )   115 - 120   2001.4

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  • Molecular mapping of the cnx2 locus involved in molybdenum cofactor biosynthesis in rice (Oryza sativa L.) Reviewed

    EA Barlaan, H Sato, J Mushika, S Taketa, M Ichii

    THEORETICAL AND APPLIED GENETICS   102 ( 4 )   540 - 544   2001.3

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  • Molecular mapping of a fertility restoration locus (Rfm2) for cytoplasmic male sterility in barley (Hordeum vulgare L.). Reviewed

    Matsui, K, Y. Mano, S. Taketa, N. Kawada, T. Komatsuda

    Theoretical and Applied Genetics   102   477 - 482   2001

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  • Fixation of translocation 2A center dot 4B infers the monophyletic origin of Ethiopian tetraploid wheat Reviewed

    T Kawahara, S Taketa

    THEORETICAL AND APPLIED GENETICS   101 ( 5-6 )   705 - 710   2000.10

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  • Barley allele-specific amplicons useful for identifying wheat-barley recombinant chromosomes Reviewed

    K Murai, S Taketa, AKMR Islam, KW Shepherd

    GENES & GENETIC SYSTEMS   75 ( 3 )   131 - 139   2000.6

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  • The distribution, organization and evolution of two abundant and widespread repetitive DNA sequences in the genus Hordeum Reviewed

    S Taketa, H Ando, K Takeda, GE Harrison, JS Heslop-Harrison

    THEORETICAL AND APPLIED GENETICS   100 ( 2 )   169 - 176   2000.1

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  • Aluminum tolerance genes on the short arm of chromosome 3R are linked to organic acid release in triticale

    Jian Feng Ma, S. Taketa, Zhen Ming Yang

    Plant Physiology   122 ( 3 )   687 - 694   2000

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    DOI： 10.1104/pp.122.3.687

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  • IDENTIFICATION OF CHROMOSOMAL ARM LOCATION OF AL-TOLERANT GENES IN RYE

    MA Jian Feng, TAKETA Shin, YANG Zhen Ming, TAKEDA Kazuyoshi, MATSUMOTO Hideaki

    40   s101 - s101   1999.3

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  • IDENTIFICATION OF CHROMOSOMAL ARM LOCATION OF GENES CONTROLLING HYDROXYLATION OF PHYTOSIDEROPHORES IN BARLEY

    MA Jian Feng, TAKETA Shin, CHANG Yi-Chieh, NOMOTO Kyosuke, IWASHITA Takashi, TAKEDA Kazuyoshi, MATSUMOTO Hideaki

    40   s96 - s96   1999.3

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  • Detection of Hordeum marinum genome in three polyploid Hordeum species and cytotypes by genomic in situ hybridization Reviewed

    S Taketa, H Ando, K Takeda, R von Bothmer

    HEREDITAS   130 ( 2 )   185 - 188   1999

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  • Biosynthesis of phytosiderophores in several Triticeae species with different genomes

    Jian Feng Ma, Shin Taketa, Yi Chieh Chang, Kazuyoshi Takeda, Hideaki Matsumoto

    Journal of Experimental Botany   50 ( 334 )   723 - 726   1999

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    DOI： 10.1093/jexbot/50.334.723

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  • Genes controlling hydroxylations of phytosiderophores are located on different chromosomes in barley (Hordeum vulgate L.)

    Jian Feng Ma, Shin Taketa, Yi Chieh Chang, Takashi Iwashita, Hideaki Matsumoto, Kazuyoshi Takeda, Kyosuke Nomoto

    Planta   207 ( 4 )   590 - 596   1999

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    DOI： 10.1007/s004250050522

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  • Genetic variation in barley of crossability with wheat and its quantitative trait loci analysis

    S Taketa, H Takahashi, K Takeda

    EUPHYTICA   103 ( 2 )   187 - 193   1998

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  • Expression of dominant marker genes of barley in wheat-barley hybrids Reviewed

    S Taketa, K Takeda

    GENES & GENETIC SYSTEMS   72 ( 2 )   101 - 106   1997.4

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  • C-banding analysis on wild Emmer (Triticum dicoccoides Korn) strains with and without spontaneous reciprocal translocations Reviewed

    S Taketa, T Kawahara

    THEORETICAL AND APPLIED GENETICS   92 ( 2 )   173 - 178   1996.2

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  • CHROMOSOME CONSTITUTION OF WHEAT × BARLEY HYBRIDS AND THEIR BACKCROSS PROGENY, AND EXPRESSION OF BARLEY GENES

    TAKETA S., TAKEDA K.

    70 ( 6 )   716 - 716   1995.12

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  • High crossability of wild barley (Hordeum spontaneum C. Koch) with bread wheat and the differential elimination of barley chromosomes in the hybrids Reviewed

    S Taketa, J Kato, K Takeda

    THEORETICAL AND APPLIED GENETICS   91 ( 8 )   1203 - 1209   1995.12

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Books

  • 植物育種学辞典，日本育種学会編

    培風館  2005 

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  • Taketa, S, Linde-Laursen, I. and Künzel, G.. Cytogenetic diversity in barley (Hordeum vulgare). In: Diversity in barley, edited by R. von Bothmer, H. Knüpffer, T. van Hintum and K. Sato

    2003 

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MISC

  • 植物科学とサイエンスコミュニケーション Invited

    武田 真

    岡山大学附属図書館報 楷   77   2 - 5   2023.10

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  • 観賞用オオムギの開発に利用可能な遺伝子源 Invited

    武田 真

    アグリバイオ   6 ( 9 )   867 - 868   2022.8

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  • Barley albino lemma1 mutant gene and photosynthesis Invited

    Shin Taketa

    Agricultural Biotechnology   5 ( 14 )   54 - 55   2021.12

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  • P067 Distribution of defensive secondary metabolites in Hordeum genus

    Ube Naoki, Nishizaka Miho, Taketa Shin, Ishihara Atsushi

    50   85 - 85   2015.10

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  • オオムギのプロアントシアニジン欠失突然変異体(ant突然変異体)を利用した種子色が種子休眠に及ぼす影響について

    氷見英子, 武田真, 前川雅彦

    育種学研究   14   119   2012.9

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  • Interrelationship between Shoot and Root Growth of a Short-root Near Isogenic Line IL-srt1 Derived from Rice Cultivar Oochikara

    ZHAO Rengui, LIU Jian, SHIOTSU Fumitaka, TOYOTA Masanori, KUSUTANI Akihito, TAKETA Shin, ICHII Masahiko

    ( 42 )   18 - 19   2005.12

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    Language：Japanese   Publisher：The Crop Science Society of Japan  

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  • Physiological Characteristics of Short-Root Mutant IL-srt1 Induced from Rice Cultivar Oochikara

    ZHA R., LIU J., SHIOTSU F., NAKAKITA T., TOYOTA M., KUSUTANI A., TAKETA S., ICHII M.

    ( 41 )   18 - 19   2004.12

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  • コムギとオオムギの属間交雑およびオオムギ属の系統分化に関する細胞遺伝学的研究．

    武田 真

    育種学研究５   5   109 - 116   2003

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  • Development of molecular markers tightly linked to the naked caryopsis gene (nud) in barley

    KIKUCHI S., TAKEDA S., KAWASAKI S., ICHII M.

    4   271 - 271   2002.8

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  • The effects of auxin on lateral root initiation and root gravitropism in a lateral rootless mutant Lrt1 of rice (Oryza sativa L. )

    4   354 - 354   2002.8

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  • Phylogenetic analysis of the genus Hordeum based on tandem repetitive Afa-family sequences

    CHODA M., TAKETA S., ICHII M.

    4   397 - 397   2002.8

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  • Molecular mapping of a gene for Si uptake in rice

    ARIMURA M., TAKEDA S., MA J. F., WU G. F., TAMAI K., ICHII M.

    4 ( 1 )   73 - 73   2002.3

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  • Improvement of alkaline soil tolerance in rice (1) Genotypic variation in response to sodium carbonate stress.

    ICHII M., NAKAGAWA Y., TAKETA S.

    2 ( 2 )   182 - 182   2000.9

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  • The influence of a spring habit gene, Vrn-D1, on heading time in wheat

    KATO H., TAKETA S., BAN T., IRIKI N., MURAI K.

    2 ( 2 )   238 - 238   2000.9

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  • Characterization of roothair defective mutant in rice

    ICHII M., KAWAMURA Y., YANG L., TAKEDA S.

    2 ( 1 )   137 - 137   2000.4

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  • Production of wheat-wild barley (Hordeum vulgare ssp. spontaneum (C. Koch.) Thell.) chromosome addition lines

    TAKEDA S., TAKEDA K.

    2 ( 1 )   150 - 150   2000.4

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  • The RAPD marker which can distinguish male sterile and maintainer line derives from mitochondria in indica rice

    HONG D. L., TAKEDA S., ICHII M.

    2 ( 1 )   182 - 182   2000.4

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  • Effects of the additional barley chromosome 5H on heading characters in wheat

    KAWAHARA T., TAKETA M., MURAI K.

    2 ( 1 )   218 - 218   2000.4

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  • 根毛量に関するイネ突然変異体の特性解析(予報)

    一井 眞比古, 河村 裕行, 武田 真

    根の研究 = Root research   8 ( 4 )   135 - 135   1999.12

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  • Preferential occurrence of specific R-D chromosome constitutions in stable hexaploid progenies of the hybrid between hexaploid triticale and bread wheat Reviewed

    Shin Taketa, Tetsuya Nakazaki, Shoji Shigenaga, Hirotada Yamagata

    The Japanese Journal of Genetics   66 ( 5 )   587 - 596   1991

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Presentations

  • Barley albino lemma 1 mutants

    Taketa, Shin

    The 3rd Barley Mutant Conference  2023.10.9 

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  • Barley albino lemma 1 mutations reduce spike photosynthesis and seed weight

    Shin Taketa

    The 13th Triennial Meeting of the International Society for Seed Science  2021.8.9 

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    Event date： 2021.8.13

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  • オオムギのプロアントシアニジン欠失突然変異体(ant突然変異体)を利用した種子色が種子休眠に及ぼす影響について

    氷見英子, 武田真, 前川雅彦

    育種学研究  2012.9.14 

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  • Isolation and structural analyses of seed-specific polyphenol oxidase genes in barley

    Kanako Matsuki, Satoko Amano, Eiko Himi, Kazuhiko Noda, Kazuyoshi Takeda, Shin Taketa

    GENES & GENETIC SYSTEMS  2007.12  GENETICS SOC JAPAN

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  • Introduction to Wheat Plants Invited

    Taketa Shin

    Abstracts of Meeting of the CSSJ  2006  CROP SCIENCE SOCIETY OF JAPAN

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  • オオムギの極短芒突然変異体の遺伝・細胞学的解析

    武田 真, 仁科友希, 白神美津穂

    日本育種学会, 育種学研究26 (別2) 38  2024.9.19 

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  • オオムギにおけるホルダチン合成酵素の同定

    宇部尚樹, 石原 亨, 藪田行哲, 武田真, 加藤康夫, 野村泰治

    日本農芸化学会, 東京  2024.3.27 

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  • コムギ・エギロプス属における穎果と穎の接着・非接着に関する遺伝変異―オオムギ属との比較

    武田 真

    日本育種学会  2024.3.17 

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  • エピゲノム制御を介したオオムギの芒形成機構の解析

    中村光希, 菊池優一, 白神美津穂, 小竹敬久, 武田真, 池田陽子

    日本育種学会第144回講演会, 神戸  2023.9.16 

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  • エピゲノム制御が担うオオムギの芒形成

    中村光希, 菊池優一, 白神美津穂, 小竹敬久, 武田真, 池田陽子

    第1回植物生理若手の会, 千葉  2023.8.9 

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  • オオムギの芒形成を担うエピゲノム制御因子

    中村光希, 菊池優一, 白神美津穂, 小竹敬久, 武田真, 池田陽子

    第64回日本植物生理学会，仙台(ハイブリッド)  2023.3.10 

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  • Barley diversity preserved in genebanks is indispensable for gene hunting and pre-breeding. Invited

    Barley diversity, preserved in genebanks is indispensable for gene hunting, pre-breeding

    Use of Genetic Resources in Breeding for Climate Change in the Nordic Region– Why Research and Innovation Do Matter, NordGen PPP Conference  2023.2.2 

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  • オオムギ・コムギの芒の長さを決める遺伝子

    武田 真

    イネ科作物の穂先突起の形態を制御する遺伝子 岡山大学植物研 共同研究拠点ワークショップ  2022.12.21 

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  • オオムギ葉身形成におけるWOX3遺伝子の機能解析

    吉川貴徳, 久野 裕, 桧原健一郎, Jilu Nie, 田中裕己, 伊藤純一, 武田 真

    ムギ類研究会  2022.12.17 

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  • オオムギの芒形成に関わる推定エピゲノム制御因子の解析

    中村光希, 菊池優一, 白神美津穂, 武田 真, 池田陽子

    NGS発生生物学現場の会2022  2022.12.16 

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  • Functional characterization of a putative epigenetic regulator that affects awn elongation in barley

    Nakamura, K., Kikuchi, Y., Shiraga, M., Kotake, T., Taketa, S., Ikeda, Y.

    CHSA meeting on Integrative Epigenetics in Plants  2022.12.15 

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  • オオムギの芒形成に関わるエピゲノム制御因子の解析

    中村光希, 菊池優一, 白神美津穂, 小竹敬久, 武田 真, 池田陽子

    日本植物学会第86回大会  2022.9.15 

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  • オオムギ丸粒突然変異体の遺伝解析

    武田 真, 白神美津穂, 大関美香, 五月女敏範

    日本育種学会第141回大会  2022.3.20 

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  • オオムギ内穎裂開突然変異体の解析

    武田 真, 五月女敏範, 吉川貴徳

    日本育種学会第139回講演会  2021.9.23 

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  • オオムギ白穎(albino lemma 1)変異は、GLK2転写因子の異常で引き起こされ、種子重を低下させる

    武田 真, 服部桃子, 高見常明, 坂本 亘

    日本育種学会第139回講演会  2021.3.19 

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  • オオムギ 多節矮性変異体を用いた葉間期制御に関わる3遺伝子座の同定

    桧原 健一郎, 味谷雅之, 桧原(松尾, 直子, 三村真生, 吉川貴徳, スズキマサハル, 草場 信, 武田 真, 伊藤 純一

    日本育種学会第139回講演会  2021.3 

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  • オオムギにおけるフラボノイド型ファイトアレキシン

    宇部尚樹, 勝山由郁, 假谷佳祐, 手林慎一, 武田真, 上野琴巳, 石原亨

    日本農芸化学会中四国支部大会  2020.9 

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  • オオムギ MANY-NODED DWARF遺伝子群による葉間期制御

    桧原 健一郎, 武田 真, 伊藤 純一

    日本育種137回講演会 コロナのため中止で、要旨のみ発表  2020 

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  • 醸造特性の異なるビール大麦２品種の未熟種子における転写産物のRNA-seq解析 Invited

    武田 真, 高橋秀和, 持田恵一, 石毛太一郎, 矢嶋俊介

    東京農業大学 生物資源ゲノム解析拠点 研究報告会  2019.2.15 

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  • イネとオオムギの形態の違い

    武田 真

    日本育種学会2018年秋期ワークショップ  2018.9.22 

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Research Projects

  • ビール大麦の水感受性因子をターゲットとした遺伝子解析

    2023.07 - 2024.06

    ビール酒造組合 共同研究費 

    武田真

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    Authorship：Principal investigator 

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  • オオムギ縞萎縮病の新規抵抗性遺伝子の単離と機能解明

    Grant number：23H02184  2023.04 - 2027.03

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    武田 真

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    Grant amount：\16770000 （ Direct expense: \12900000 、 Indirect expense：\3870000 ）

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  • オオムギ縞萎縮病の新規抵抗性遺伝子の単離と機能解明

    Grant number：23K26877  2023.04 - 2027.03

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    武田 真

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    Grant amount：\16770000 （ Direct expense: \12900000 、 Indirect expense：\3870000 ）

    オオムギ縞萎縮病は土壌伝染するウイルスが引き起こす深刻な病害である。本病に感染すると葉が黄色く枯れ上がり生育不良となり、収量と品質が大きく低下する。本病ウイルスは農薬による防除が現実的でない。実際的な防除法は本病に対するオオムギの持つ抵抗性遺伝子を活用することである。これまでrym5抵抗性遺伝子を多用し続けたため、ウイルスの変異株(Ⅲ型)が出現した。新規の縞萎縮病抵抗性遺伝子rym3はⅢ型変異株に抵抗性である。その分子実体を特定し、抵抗性機構を解明し、持続的なウイルス病抵抗性育種の道筋を付けることが本研究の目的である。
    これまでの研究で新規の抵抗性遺伝子rym3は5H染色体の0.4 cMの範囲に位置することがわかっている。今年度は抵抗性品種イシュクシラズと罹病性品種ニューサチホゴールデンの交配F2世代2,310個体を播種し、各個体からDNAを抽出し、rym3を挟み込むDNAマーカーを検定し、この区間で組換を起こした60個体(2.6%)を選抜した。組換個体は植木鉢に定植を終え、網室で栽培しており、2024年度春期に種子を収穫する予定である。その後、脱穀し、一部の種子はrym3縞萎縮病検定用のⅢ型ウイルス汚染圃場のある栃木県農業試験場に送付し、共同研究者に秋期に播種と、翌2025年3月上旬のrym3抵抗性検定を実施頂き、データを共有する予定である。
    さらに、抵抗性品種サチホゴールデンと罹病性品種ミホゴールデンの交配から育成した、rym3遺伝子近傍だけが異なり、それ以外は遺伝的にほぼ等しい系統を交配とマーカー選抜で養成した。それら系統対のゲノム配列を次世代シーケンサーで解読した。現在、データ解析の専門家との共同研究により、rym3の候補遺伝子を探索している

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  • オオムギの健康性分である水溶性食物繊維(βーグルカン)を高める遺伝子の解明

    Grant number：13  2022.10 - 2023.09

    両備てい園記念財団  生物学研究奨励賞 

    武田 真

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  • ビール大麦の品質・生産性向上のための遺伝育種学的多様性の研究

    2022.06 - 2023.05

    ビール酒造組合 

    武田 真

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  • コムギ長葉毛遺伝子の単離と発現解析

    Grant number：31C2001  2022.04 - 2023.01

    鳥取大学乾燥地研究センター 

    武田 真

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  • 土壌伝染性ウイルスで起こる「大麦縞萎縮病」に対するオオムギ抵抗性遺伝子の多様性解析

    2020.09 - 2021.03

    八雲環境科学財団 令和2年度助成  令和2年度環境研究助成  特定研究

    武田 真

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    Authorship：Principal investigator  Grant type：Competitive

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  • Genetic analysis of a leafy lemma mutant in barley

    Grant number：19K05971  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Taketa Shin

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    Grant amount：\4420000 （ Direct expense: \3400000 、 Indirect expense：\1020000 ）

    A leafy lemma mutant of barley was dissected by positional cloning approaches. We clarified that three genes are involved in its drastic conversion of awns to the leafy lemmas. Positional cloning approaches in combination with barley genome sequence data clarified causal genes (a total of three).

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  • Photosysnthetic activity of spikes measured by albino lemma mutants in barley

    Grant number：16K07556  2016.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Taketa Shin

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    Grant amount：\4810000 （ Direct expense: \3700000 、 Indirect expense：\1110000 ）

    The albino lemma (alm1) mutant in barley is characterized by chlorophyll-less hulls except for small green areas at the tips. By means of positional cloning and candidate gene sequencing, we revealed the causal gene of ALM1. Photosynthesis measurements using an isogenic pair of wild type and alm1 mutant revealed that leaf photosynthesis did not differ, but that spike photosynthesis was reduced 34% in alm1 mutant. Grain weight of alm1 mutant was reduced 10-21% compared to wild type. These results suggest significant contribution of hull photosynthesis to grain filling in barley.

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  • Cloning of a gene controlling awn length in barley

    Grant number：25450008  2013.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    TAKETA SHIN

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    Grant amount：\5200000 （ Direct expense: \4000000 、 Indirect expense：\1200000 ）

    Molecular cloning of a short awn gene in barley was attempted by map-based cloning approaches. For a short awn gene (sca), we have identified several candidate genes.

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  • Isolation and characterization of beta-glucan synthase genes

    Grant number：21580007  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    TAKETA Shin, TONOOKA Takuji, TSUMURAYA Yoichi, HARUYAMA Naoto, JOBLING SA

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    Grant amount：\4810000 （ Direct expense: \3700000 、 Indirect expense：\1110000 ）

    (1, 3 ; 1, 4)-s-D-glucans(mixed-linkage glucans) are found in tissues of members of Poaceae(grasses), and are particularly high in barley(Hordeum vulgare) grains have beneficial effects on health. Our present results demonstrate that, among the seven CslF and one CslH genes present in the barley genome, HvCslF6 has a unique role and is the key determinant controlling biosynthesis of(1, 3 ; 1, 4)-s-D-glucans.

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  • Molecular genetic analysis on the expression mechanisms of the covered and naked caryopsis in barley

    Grant number：18580006  2006 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    TAKETA Shin

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    Grant amount：\3750000 （ Direct expense: \3300000 、 Indirect expense：\450000 ）

    通常のオオムギは, 脱穀しても実と殻が糊物質で接着していて分離できない, "皮麦"である。しかし, 一部のオオムギは, 頴果と頴が容易に分かれる変種で,"はだか麦"とよばれる。オオムギの頴果が皮性・裸性のどちらになるかは, 単一の遺伝子座で決まる単純なメンデル遺伝に従い, 皮性が裸性に対して優性である。オオムギの重要形質の1つである皮性・裸性を決める遺伝子の分子的な実体はこれまで不明であり, 実と殻が接着したり, 分離する機構も全くわかっていなかった。われわれのグループはポジショナルクローニングにより, オオムギの皮性・裸性の表現型を制御する遺伝子がERF転写因子であることを世界で初めて明らかにした。

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  • Moleculargenetic investigation on silica uptake in rice

    Grant number：15380007  2003 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    ICHII Masahiko, TAKETA Shin

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    Grant amount：\10200000 （ Direct expense: \10200000 ）

    A mutant with low Si uptake was induced by chemical mutagen treatment. We named the mutant GR1, because it was selected based on the resistance against germanium (Ge), an analogue of silicon (Si) with harmful effect on plants. Molecular mapping showed that the causal gene for low silicon uptake in GR1 was located on the long arm of rice chromosome 2.We determined Si content in the mutant and the wild type by chemical analysis and found that the mutant showed a range of 11-43% of Si content of the wild type in different plant organs studied. Interestingly, the mutant showed an increased level of lignin compared to the wild type; we ascribed this elevated lignin content to "compensation effect" which tends to retain firmness of the plant body. GR1 was more susceptible blast disease and insect attacks. There was no remarkable difference in plant mass productivity upon cultivation in a field, but digestibility of the whole-crop-silage by cows was similar between the mutant and wild type. Thus, the reduced level of Si in the mutant did not result in improvement in digestibility by cows. To know the root parts that contribute to Si uptake, we analyzed various rice root mutants defective in lateral roots or root hairs. Our analysis showed that Si was mainly absorbed through lateral roots and that root hairs did not show significant contribution to Si uptake in rice.

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  • Isolation and characterization of the barley gene controlling hulled or naked caryopsis

    Grant number：15580007  2003 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    TAKETA Shin

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    Grant amount：\3900000 （ Direct expense: \3900000 ）

    In barley, the hulled or naked caryopsis trait is one of essential agronomic characters because it is closely associated with the utility of harvested barley grains either as malting/feeding purpose or human food.. We attempted isolation and characterization of the barley gene controlling hulled or naked caryopsis using a positional cloning approach. First, we developed linked molecular markers by using AFLP and bulked segregant analysis. Putative linked AFLP bands were sequenced to develop PCR based markers. Then, large segregating populations were analyzed for a fine mapping of the gene. This mapping resulted in the state where the gene was flanked by two co-dominant markers with genetic distance of 0.6 cM in proximal side and 0.06 cM in distal side, respectively. Next, bioinformatics were applied to identify microsynteny between the naked caryopsis gene in the barley genome and the rice genome. We successfully identified microcollinearity with the long arm of rice chromosome 6. Rice genome sequence information was effectively used to further narrow down the naked caryopsis gene in the barley genome. We then started the physical map construction of the entire locus. We have successfully constructed the barley BAC contig of about 500 kb, which spans the hulled or naked caryopsis gene. On the bases of available information, we have identified a candidate gene for the hulled or naked caryopsis character in barley. Expression analysis seems to support the identity of this gene. We also analyzed induced naked caryopsis mutants and found non-synonymous mutations, which solidified our conclusion. We conclude that naked barley in the world has a single origin, which appears to have occurred in the Middle East. On the basis of allelic variation of a molecular marker that was tightly linked to the naked caryopsis gene, we elucidated two independent migratory routes of naked barley into East Asia.

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  • 分子細胞学的手法によるオオムギ野性種の系統分化の解析

    Grant number：09760006  1997 - 1998

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    武田 真

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    Grant amount：\1700000 （ Direct expense: \1700000 ）

    今年度はオオムギ属倍数種(四倍種および六倍種)について二種類のリポソームRNA遺伝子、5Sおよび18S-25SrDNAの物理マッピングを行い、オオムギ属倍数種の系統進化の解明を試みた。rDNAのパターンから以下のことが結論される。(1)栽培オオムギと同じゲノムを持つとされるH.bulbosumの四倍体は同質四倍体である。(2)Yゲノムを持つH.murinumの六倍体は四倍体と二倍体のサイトタイプの交雑によって成立したと考えられる。 (3)Xゲノムを持つH.marinumの四倍体は単純な同質四倍体ではなく、部分異質四倍体である。(4)四倍種のH.capense,H.secalinumおよび六倍種のH.brachyantherum6xにはXゲノムが含まれる。(5)北アメリカ大陸に分布するH.jubatumは5対の染色体にrDNAのシグナルを持ち、アジア大陸のH.roshevitziiとH.brachyantherum2xの交雑によって成立したと推定される。また、中央アメリカに分布するH.guatemalenseおよび南アメリカ大陸に分布するH.fuegianumはH.jubatumに近縁と考えられる。(6)北アメリカ大陸に分布するH.depressumは4対の染色体にだけrDNAシグナルを持ち、二倍種のH.intercedensとH.pusillumの交雑から起源したと推定される。(7)北アメリカ大陸の六倍種であるH.arizonicumはH.jubatulmとH.pusillumの交雑に由来すると推定される。
    従来オオムギ属は二倍体に見出される,H,I,XおよびYの4基本ゲノムに対応する4グループに分類されていた。しかし、本研究から、HゲノムとXゲノムを併せ持つ第5のグループが存在することが実証された。さらに、これまで推定困難であった倍数種の祖先種についてもかなり明確に推定することができた。これらのことから、rDNAはオオムギ属の系統進化を解明するのに有用な染色体マーカーであると結論される。

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  • 染色体工学的手法によるコムギにオオムギ第5染色体を添加した系統の育成

    Grant number：08760006  1996

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    武田 真

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    Grant amount：\1000000 （ Direct expense: \1000000 ）

    まず、不稔遺伝子の染色体上での物理的位置を推定するため、オオムギ第5染色体(1H)長腕の関与する相互転座系統(3系統)とコムギとの交雑から、転座第5染色体をコムギに導入した系統を育成し、切断点と稔性との関係を検討した。その結果、第5染色体長腕の動原体から約50%までの領域を導入した系統は正常な種子稔性を示すこと、また、第5染色体の長腕の末端から5%が他の染色体(第2染色体)に転座した染色体を添加しても種子稔性は正常であることがわかった。これらのことから、オオムギ第5染色体上の不稔を引き起こす遺伝子は長腕上の50〜95%の領域に位置することが明らかになった。次に、第5染色体によって誘発される不稔性は、オオムギ第6染色体(6H)によって部分的に緩和されることがわかっているが、その不稔緩和遺伝子は第6染色体の長腕に存在することを明らかにした。このように、第5染色体長腕の動原体から50%の領域および末端から5%の領域をコムギに導入することに成功した。現在、導入された第5染色体領域を容易に検出できる分子マーカーを検索中である。
    さらに、エギロプス(Aegilops)属由来の配偶子致死染色体の持つ染色体切断作用を利用して、不稔遺伝子を除去するための実験系統を育成した。この系統はオオムギの第5及び第6染色体に加えて配偶子致死染色体を1本ずつ持ち、雌性稔性がある。現在、戻し交雑後代の細胞学的調査によって不稔遺伝子を含むできるだけ小さい部分の欠失を起こした染色体の選抜を試みている。

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  • 分子細胞学的手法による新たなオオムギ染色体マーカーの創出

    Grant number：07760004  1995

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    武田 真

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    Grant amount：\1000000 （ Direct expense: \1000000 ）

    本研究では、オオムギの新たな染色体マーカーを分染法および分子細胞学的手法によって創出することを試みた。
    まず、野生オオムギ(Hordeum spontaneum)および栽培オオムギ(Hordeum vulgare)を各3系統供試し、C-分染法によるバンドパターンの変異を調査した。その結果、野生オオムギは3系統とも第5染色体短腕の中部に栽培オオムギではみられない鮮明なバンドを持つことが明らかになった。野生オオムギと栽培オオムギとのF_1雑種の減数分裂第一中期の染色体をC-分染したところ、野生オオムギと栽培オオムギの第5染色体短腕間で正常に対合することがわかった。このことから野生オオムギの第5染色体短腕のバンドは構造異常ではなく、多型によって生じたものであることが明らかになった。
    次に、同一の染色体核板に対して分染とゲノミックin situハイブリダイゼーションを連続的に行う方法を、四倍性コムギの一種であるTriticum abyssinicumを材料として検討した。その結果、先にin situハイブリダイゼーションを行った標本をひき続いてN-分染する方法では、鮮明なN-分染像を得ることができなかった。しかし、先にN-分染を行い、脱染色してin situハイブリダイゼーションすると、良好なシグナルが得られることが明らかになった。
    さらに、コムギにオオムギ染色体が添加された系統を供試し、ゲノミックin situハイブリダイゼーション法を適用した。コムギの全DNAを無標識とし、オオムギの全DNAをビオチンで標識し、両者を約25:1の濃度比で混合したものをプローブとし、染色体標本にin situハイブリダイゼーションさせ、シグナルを蛍光検出した。その結果、オオムギ染色体とコムギ染色体を識別することができた。現在、混合する濃度比を変化させ、バンド状のパターンが得られないかどうかを検討している。

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  • ライコムギに導入されたDゲノム染色体断片の分子細胞遺伝学的手法による検出

    Grant number：06760008  1994

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    武田 真

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    Grant amount：\900000 （ Direct expense: \900000 ）

    本研究ではパンコムギとの交雑を経て育成されたライコムギ系統を蛍光in situハイブリダイゼーション法によって解析し,Rゲノム染色体とDゲノム染色体との間の転座染色体を検出することを試みた.
    まず,3D染色体短腕と3R染色体長腕とからなる転座染色体のin situハイブリダイゼーション法による検出を試みた.ライムギ(Secale cereale,Rゲノム)とDゲノムの提供親であるタルホコムギ(Aegilops squarrosa)それぞれの全DNAを抽出し,RあるいはDゲノムいずれかの全DNAをビオチンで標識し,他方の全DNAを無標識のまま約50倍量加えてin situハイブリダイゼーションを行ったところ,転座染色体を明瞭に検出できた.つぎに,染色体対合の分析から転座を持つことはわかっているが,転座点が同定できていないライコムギ系統Bronco 90をin situハイブリダイゼーション法で解析した。タルホコムギの全DNAをビオチン標識しライムギの全DNAを無標識のまま加えてin situハイブリダイゼーションを行ったところ,1対のライムギ染色体の長腕の末端よりの約1/10の部分にシグナルが認められ,この部分がDゲノム由来であることがわかった.しかし,逆の標識を行った場合にはこの転座は検出できなかった.したがって,in situハイブリダイゼーション法は,プローブの標識を適切に行えば,微少な転座でも切断点を正確に同定できることが明らかになった.

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  • 青陵高校生に対面で研究紹介20分を2回

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    岡山大学資源植物科学研究所  2022.7.27

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