Faculty Profiles - HIRASAWA Akira

Papers - HIRASAWA Akira

Division display 381 - 400 of about 403 ／ All the affair displays >>

Differentially regulated genes as putative targets of amplifications at 20q in ovarian cancers Reviewed

Watanabe T, Imoto I, Katahira T, Hirasawa A, Ishiwata I, Emi M, Takayama M, Sato A, Inazawa J

Jpn J Cancer Res 93 ( 10 ) 1114-1122 2003

子宮体癌におけるMicrosatellite Instabilityと薬剤感受性試験によるcisplatin感受性との関連

進, 伸幸, 青木, 大輔, 阪埜, 浩司, 平尾, 健, 岩田, 卓, 平沢, 晃, 金杉, 優, 鈴木, 直, 菅野, 康吉, 宇田川, 康博, 吉村, 泰典, 野澤, 志朗

日本産科婦人科学会雑誌 55 ( 2 ) 186 - 186 2003

Differentially regulated genes as putative targets of amplifications at 20q in ovarian cancers.

Takafumi Watanabe, Issei Imoto, Tomoyuki Katahira, Akira Hirasawa, Isamu Ishiwata, Mitsuru Emi, Masaomi Takayama, Akira Sato, Johji Inazawa

Japanese journal of cancer research : Gann 93 ( 10 ) 1114 - 22 2002.10

Language：English Publishing type：Research paper (scientific journal) Publisher：BUSINESS CENTER ACADEMIC SOCIETIES JAPAN

Frequent amplification of DNA at 20q or part of 20q has been demonstrated by comparative genomic hybridization in ovarian cancer (OC), but the genetic target(s) of these amplification events remain unknown. We examined copy-number changes with respect to six candidate genes, E2F1 (20q11.2), TGIF2 (20q11.2), AIB1 (20q12), PTPN1 (20q13.1), ZNF217 (20q13.2), and BTAK (20q13), and then measured transcription levels of each candidate in 18 OC cell lines. Three distinct cores of amplification were identified: 20q11.2, harboring E2F1 and TGIF2 (region I; 1 of 18 cell lines, 5.6%); 20q13.1, harboring PTPN1 (region II; 5 lines, 27.8%); and 20q13.2, harboring ZNF217 and BTAK (region III; 6 lines, 33.3%). Among the six genes examined, expression levels of PTPN1 and ZNF217 were significantly correlated with absolute copy-number, and those of PTPN1 and TGIF2 were significantly correlated with copy-number relative to the centromere of chromosome 20 (20cen). Among 19 primary OCs examined, moreover, we observed amplification of TGIF2, PTPN1 and ZNF217 in five (26.3%), ten (52.6%), and twelve (63.2%) tumors, respectively. Expression levels of PTPN1 and ZNF217 were significantly correlated with their copy-numbers in those primary OCs. Our results suggest that 20q amplifications in OCs can be extensive and complex, probably due to synergistic or non-synergistic amplification of separate regions of 20q, involving multiple, independently amplified targets.

Web of Science

PubMed

researchmap

婦人科癌とDNAミスマッチ修復遺伝子―HNPCCに関する婦人科癌を中心として―

Aoki Daisuke, Hirasawa Akira, Susumu Nobuyuki, Nozawa Shirou

産婦人科の実際 51 ( 10 ) 1361-1368 - 1368 2002.10

Differentially regulated genes as putative targets of amplifications at 20q in ovarian cancers

T Watanabe, Imoto, I, T Katahira, A Hirasawa, Ishiwata, I, M Emi, M Takayama, A Sato, J Inazawa

JAPANESE JOURNAL OF CANCER RESEARCH 93 ( 10 ) 1114 - 1122 2002.10

Language：English Publishing type：Research paper (scientific journal) Publisher：BUSINESS CENTER ACADEMIC SOCIETIES JAPAN

Frequent amplification of DNA at 20q or part of 20q has been demonstrated by comparative genomic hybridization in ovarian cancer (OC), but the genetic target(s) of these amplification events remain unknown. We examined copy-number changes with respect to six candidate genes, E2F1 (20q11.2), TGIF2 (20q11.2), AIB1 (20q12), PTPN1 (20q13.1), ZNF217 (20q13.2), and BTAK (20q13), and then measured transcription levels of each candidate in 18 OC cell lines. Three distinct cores of amplification were identified: 20q11.2, harboring E2F1 and TGIF2 (region I; I of 18 cell lines, 5.6%); 20q13.1, harboring PTPN1 (region H; 5 lines, 27.8%); and 20q13.2, harboring ZNF217 and BTAK (region 111; 6 lines, 33.3%). Among the six genes examined, expression levels of PTPN1 and ZNF217 were significantly correlated with absolute copy-number, and those of PTPN1 and TGIF2 were significantly correlated with copy-number relative to the centromere of chromosome 20 (20cen). Among 19 primary OCs examined, moreover, we observed amplification of TGIF2, PTPN1 and ZNF217 in five (26.3%), ten (52.6%), and twelve (63.2%) tumors, respectively. Expression levels of PTPN1 and ZNF217 were significantly correlated with their copy-numbers in those primary OCs. Our results suggest that 20q amplifications in OCs can be extensive and complex, probably due to synergistic or non-synergistic amplification of separate regions of 20q, involving multiple, independently amplified targets.

Web of Science

researchmap

Expression of cIAP1, a target for 11q22 amplification, correlates with resistance of cervical cancers to radiotherapy. Reviewed International journal

Issei Imoto, Hitoshi Tsuda, Akira Hirasawa, Masahiko Miura, Masaru Sakamoto, Setsuo Hirohashi, Johji Inazawa

Cancer research 62 ( 17 ) 4860 - 6 2002.9

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER ASSOC CANCER RESEARCH

Inhibition of, or increased resistance to, apoptosis is a common property of cancer cells. This means that a constitutive activation of antiapoptotic molecules via genetic or epigenetic mechanisms, including gene amplification, may well be involved in carcinogenesis. Recently we reported that cIAP1, an inhibitor of apoptosis, is overexpressed through 11q22 amplification in cell lines derived from esophageal squamous cell carcinomas and is associated with resistance of esophageal squamous cell carcinomas to drug-induced apoptosis (I. Imoto et al. Cancer Res., 61: 6629-6634, 2001). Because amplification of 11q22 has been implicated in other malignancies also, including cervical squamous cell carcinomas (CSCCs), we attempted to correlate amplification and overexpression of cIAP1 with radiation sensitivity in CSCC-derived cell lines and primary CSCC tumors. In the nine cell lines we examined, two showed amplification and consistent overexpression of cIAP1, as well as significant resistance to radiation-induced cell death as compared with lines showing no cIAP1 amplification. Immunohistochemical analysis of 70 primary CSCCs from patients treated only with radiotherapy demonstrated that both overall survival and local recurrence-free survival was significantly poorer among patients with tumors showing high levels of nuclear cIAP1 staining than among patients whose tumors revealed little or no nuclear cIAP1. Multivariate analysis showed nuclear cIAP1 staining to be an independent predictive factor for local recurrence-free survival after radiotherapy among patients with CSCC. These findings demonstrate that cIAP1 may play an important role in the development/progression of this disease and that cIAP1 could be a novel predictive marker for resistance to radiotherapy in individual CSCC patients.

Web of Science

PubMed

researchmap

Expression of cIAP1, a target for 11q22 amplification, correlates with resistance of cervical cancers to radiotherapy

Imoto, I, H Tsuda, A Hirasawa, M Miura, M Sakamoto, S Hirohashi, J Inazawa

CANCER RESEARCH 62 ( 17 ) 4860 - 4866 2002.9

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER ASSOC CANCER RESEARCH

Inhibition of, or increased resistance to, apoptosis is a common property of cancer cells. This means that a constitutive activation of antiapoptotic molecules via genetic or epigenetic mechanisms, including gene amplification, may well be involved in carcinogenesis. Recently we reported that cIAP1, an inhibitor of apoptosis, is overexpressed through 11q22 amplification in cell lines derived from esophageal squamous cell carcinomas and is associated with resistance of esophageal squamous cell carcinomas to drug-induced apoptosis (I. Imoto et al. Cancer Res., 67: 6629-6634, 2001). Because amplification of 11q22 has been implicated in other malignancies also, including cervical squamous cell carcinomas (CSCCs), we attempted to correlate amplification and overexpression of cIAP1 with radiation sensitivity in CSCC-derived cell lines and primary CSCC tumors. In the nine cell lines we examined, two showed amplification and consistent overexpression of cIAP1, as well as significant resistance to radiation-induced cell death as compared with lines showing no cIAP1 amplification. Immunohistochemical analysis of 70 primary CSCCs from patients treated only with radiotherapy demonstrated that both overall survival and local recurrence-free survival was significantly poorer among patients with tumors showing high levels of nuclear cIAP1 staining than among patients whose tumors revealed little or no nuclear cIAP1. Multivariate analysis showed nuclear cIAP1 staining to be an independent predictive factor for local recurrence-free survival after radiotherapy among patients with CSCC. These findings demonstrate that cIAP1 may play an important role in the development/progression of this disease and that cIAP1 could be a novel predictive marker for resistance to radiotherapy in individual CSCC patients.

Web of Science

researchmap

子宮体癌に対する手術療法

HIRASAWA AKIRA

日本婦人科腫瘍学会雑誌 20 ( 1 ) 26-32 - 32 2002

子宮内膜癌におけるマイクロサテライト不安定性とhMLH1蛋白発現低下との関連

阪埜, 浩司, 進, 伸幸, 平沢, 晃, 金杉, 優, 岩田, 卓, 青木, 大輔, 菅野, 康吉, 野澤, 志朗

家族性腫瘍 2 ( 2 ) A43 - A43 2002

子宮内膜癌におけるマイクロサテライト不安定性とhMLH1蛋白発現低下との関連

阪埜, 浩司, 進, 伸幸, 平沢, 晃, 金杉, 優, 岩田, 卓, 鈴木, 直, 青木, 大輔, 菅野, 康吉, 吉村, 泰典, 野澤, 志朗

日本産科婦人科学会雑誌 54 ( 2 ) 325 - 325 2002

遺伝性非腺腫性大腸癌(HNPCC)と婦人科癌

Banno Kouji, Nikata Masanobu, Hirasawa Akira, Susumu Nobuyuki, Aoki Daisuke, Udagawa Yasuhiro, Nozawa Shirou

産婦人科の実際 50 ( 12 ) 1819-1826 - 1826 2001.11

卵巣明細胞腺癌におけるPAI-1発現の生物学的意義

Komiyama Shinichi, Aoki Daisuke, Kanasugi Masaru, Hirasawa Akira, Susumu Nobuyuki, Nozawa Shirou

日本分子腫瘍マーカー研究会誌 16 48-49 2001.9

最近当科で経験したadenoma malignumの3例

HIRASAWA AKIRA

日本産婦人科学会東京地方部会誌 50 94-98 2001

わが教室における腫瘍マーカー検査

Hirasawa Akira, Aoki Daisuke, Udagawa Yasuhiro, Nozawa Shiro

産婦人科治療 79 ( 6 ) 680-685 - 685 1999.12

Genetic analysis of chromosome 17 and 1 in cervical cancers using fluorescencein situ hybridization and RFLP-Southern Blot

Susumu Nobuyuki, Tsuda Hitoshi, Aoki Daisuke, Udagawa Yasuhiro, Hirasawa A, Kanasugi Masaru, Nozawa Shiro

International Journal of Gynecological Cancer 9 ( Suppl 1 ) 101-102 1999.9

The relationship between MSN-1-reactive carbohydrate antigens and α-1,2 fucosyltransferase genes expressed in endometrial adenocarcinoma

Tominaga Eiichiro, Aoki Daisuke, Hirasawa Akira, Setoh Eri, Susumu Nobuyuki, Udagawa Yasuhiro, Nozawa Shiro

International Journal of Gynecological Cancer 9 ( Suppl 1 ) 68-69 1999.9

Two soluble forms of beta 1,4-galactosyltransferase released from ovarian cancer cells and from COS-1 cells transfected with its cDNA

D Aoki, E Saitoh, Y Matsumoto, E Tominaga, A Hirasawa, N Susumu, Y Udagawa, S Nozawa

ACTA HISTOCHEMICA ET CYTOCHEMICA 32 ( 3 ) 209 - 214 1999

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY

Employing RMG-1 cells derived from human ovarian adenocarcinoma and COS-1 cells transfected with cDNA for beta 1,4-galactosyltransferase, we first investigated the intracellular localization of this enzyme anal secondly assayed separately the two types of soluble forms of it (designated as normal GalT and GAT) in their culture supernatants. As results, the binding of anti-beta 1,4-galactosyltransferase monoclonal antibody was strongly positive at the Golgi area surrounding nuclei and the staining pattern was the same between RMG-1 cells and COS-1 transformants. These data demonstrated that the cells were producing beta 1,4-galactosyltransferase and had the ability to release it into the culture medium. As for the two soluble farms released from the cells, they were simultaneously detected in the culture medium by Western blotting and enzyme immunoassay using monoclonal antibodies, i.e., Mab8628 reactive to both GAT and normal-GalT and Mab8513 specific for GAT, indicating that both were derived from RMG-1 and from the cDNA introduced into COS-1 cells. Since serum-GAT clinically reflects tumor growth more accurately or specifically than normal GalT does, RMG-1 and COS-1 cells having cDNA for beta 1,4-galactosyltransferase would be promising as sources of these enzymes in order to investigate the differences in the behaviors of normal GalT and GAT associated with ovarian malignant tumors.

DOI： 10.1267/ahc.32.209

Web of Science

researchmap

Two soluble forms of beta 1,4-galactosyltransferase released from ovarian cancer cells and from COS-1 cells transfected with its cDNA

D Aoki, E Saitoh, Y Matsumoto, E Tominaga, A Hirasawa, N Susumu, Y Udagawa, S Nozawa

ACTA HISTOCHEMICA ET CYTOCHEMICA 32 ( 3 ) 209 - 214 1999

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY

DOI： 10.1267/ahc.32.209

Web of Science

researchmap

子宮肉腫の2症例

HIRASAWA AKIRA

栃木県産婦人科医報 25 ( 1 ) 40-42 - 42 1998

診断に苦慮した子宮頸部原発絨毛癌の1例

HIRASAWA AKIRA

栃木県産婦人科医報 25 ( 2 ) 113-116 - 116 1998

　 PREV - NEXT 　

< HIRASAWA Akira >

Papers - HIRASAWA Akira