共同研究・競争的資金等の研究 - HARA EMILIO SATOSHI
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三次元骨組織迅速作製技術の構築を指向したリン脂質ナノフラグメント作製法の確立
2018年04月 - 2019年03月
公益財団法人京都技術科学センター
ハラ エミリオ・サトシ
担当区分：研究代表者
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Bioengineering bridges between Okayama and Sao Paulo Universities
2017年
岡山大学 H29岡山大学次世代研究コア形成支援事業・若手研究者育成支援事業
ハラ エミリオ・サトシ
担当区分：研究代表者
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合成微小環境を用いた軟骨組織のｉｎ ｖｉｔｒｏ構築
研究課題/領域番号：14F04106 2014年04月 - 2016年03月
日本学術振興会 科学研究費助成事業 特別研究員奨励費 特別研究員奨励費
松本 卓也, ハラ エミリオ・サトシ
配分額：2400000円 （ 直接経費：2400000円 ）
The purpose of this study was to identify new methods and culture conditions to enhance chondrogenic differentiation of human bone marrow-derived mesenchymal stem/progenitor cells (hBMSCs) by applying chemical, physical and biological cues, including overexpression of DNA methyltransferases (DNMTs) in cells. We attempted to fabricate a chemically- and physically-controlled microenvironment using materials (e.g., hydrogels) that recapitulate native cell niche characteristics to optimally modulate the chondrogenic differentiation of BMSCs. Besides the physical and chemical stimulations, we also evaluated the chondrogenic differentiation of hBMSCs after overexpression of DNMTs that eventually induce DNA methylation at CPG rich regions of key genes. To study the effect of DNMTs on chondrogenesis of hBMSCs, we transfected overexpression vectors of DNMT3A, DNMT3B and the pcDNA control in hBMSCs, and culture the cells in a 3D micromass culture system with growth factors and glucocorticoids.
Additionally, since in vitro synthesized cartilage tissue generally undergoes through mineralization, we also attempted to understand the mechanisms involved in the chondrocyte death associated with initial mineralization process by using the secondary ossification center of mouse femur as in vivo model.