研究者詳細 - 大橋　俊孝

MISC - 大橋　俊孝

分割表示 147 件中 141 - 147 件目／全件表示 >>

マウスten-m/odz蛋白質の構造解析と発現

大橋俊孝, FASSLER Reinhard, 蘇衛東, ニ宮善文

日本分子生物学会年会プログラム・講演要旨集 21 614 - 614 1998年12月

Hypoxia Increases Transforming Growth Factor-β1 Concomitantly with Types I and III Collagen without Further Enhancement by Reoxygenation in Cultured Rat Cardiac Fibroblasts

NUNOYAMA Hiroshi, KUSACHI Shozo, NINOMIYA Yoshifumi, OOHASHI Toshitaka, KONDO Jun, MURAKAMI Masahiro, MURAKAMI Takashi, OHNISHI Hiromichi, DOI Masayuki, TSUJI Takao

Connective tissue 30 ( 3 ) 207 - 211 1998年9月

記述言語：英語出版者・発行元：日本結合組織学会

The effects of oxygen concentration and reoxygenation following hypoxic culture on transforming growth factor-β1(TGF-β1)expression and types I and III collagen[α2(I)and α1(III)]mRNA expression were examined in neonatal rat cardiac fibroblasts cultured with medium containing 10% or 1% fetal calf serum at confluency. The oxygen tension was varied using a N_2-CO_2-O_2 controllable incubator. Cells were exposed to continuous hypoxia(2% O_2)or were reoxygenated(21% O_2)after hypoxic culture. The TGF-β1 protein expression level was determined by an enzyme-linked immunosorbent assay, and the expression levels of TGF-β1, α2(I)and α1(III)mRNA were measured by a Northern blot analysis. Both the TGF-β1 mRNA and protein levels were higher in the continuous hypoxic cultures than in the continuous normoxic cultures. Their levels in reoxygenation cultures were not substantially different from those in the continuous normoxic cultures. Similarly, the expressions of both α2(I)and α1(III)mRNA were enhanced in continuous hypoxic culture, and no differences were obtained between normoxic and reoxygenation cultures. The present results indicate that hypoxia induces high expression levels of TGF-β1 and types I and III collagen in rat cardiac fibroblasts and reoxygenation does not further enhance this hypoxia-induced upregulation.

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A-5 ヒトα3(IV)、α4(IV)遺伝子プロモータ-構造と組織特異的発現について

百田龍輔, 大橋俊孝, 二宮善文

Connective tissue 30 ( 2 ) 135 - 135 1998年6月