Faculty Profiles - OOHASHI Toshitaka

MISC - OOHASHI Toshitaka

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In vitro analysis of mouse ten-m/odz protein structure and it's expression

OOHASHI Toshitaka, FASSLER Reinhard, SU Wei-Dong, NINOMIYA Yoshifumi

21 614 - 614 1998.12

Hypoxia Increases Transforming Growth Factor-β1 Concomitantly with Types I and III Collagen without Further Enhancement by Reoxygenation in Cultured Rat Cardiac Fibroblasts

NUNOYAMA Hiroshi, KUSACHI Shozo, NINOMIYA Yoshifumi, OOHASHI Toshitaka, KONDO Jun, MURAKAMI Masahiro, MURAKAMI Takashi, OHNISHI Hiromichi, DOI Masayuki, TSUJI Takao

Connect Tissue 30 ( 3 ) 207 - 211 1998.9

Language：English Publisher：The Japanese Society for Connective Tissue

The effects of oxygen concentration and reoxygenation following hypoxic culture on transforming growth factor-β1(TGF-β1)expression and types I and III collagen[α2(I)and α1(III)]mRNA expression were examined in neonatal rat cardiac fibroblasts cultured with medium containing 10% or 1% fetal calf serum at confluency. The oxygen tension was varied using a N_2-CO_2-O_2 controllable incubator. Cells were exposed to continuous hypoxia(2% O_2)or were reoxygenated(21% O_2)after hypoxic culture. The TGF-β1 protein expression level was determined by an enzyme-linked immunosorbent assay, and the expression levels of TGF-β1, α2(I)and α1(III)mRNA were measured by a Northern blot analysis. Both the TGF-β1 mRNA and protein levels were higher in the continuous hypoxic cultures than in the continuous normoxic cultures. Their levels in reoxygenation cultures were not substantially different from those in the continuous normoxic cultures. Similarly, the expressions of both α2(I)and α1(III)mRNA were enhanced in continuous hypoxic culture, and no differences were obtained between normoxic and reoxygenation cultures. The present results indicate that hypoxia induces high expression levels of TGF-β1 and types I and III collagen in rat cardiac fibroblasts and reoxygenation does not further enhance this hypoxia-induced upregulation.

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COL4A3 and COL4A4 are arranged head-to-head and COL4A4 has two alternative promoters.

Momota Ryusuke, Oohashi Toshitaka, Ninomiya Yoshifumi

Connective tissue 30 ( 2 ) 135 - 135 1998.6