MISC - OOHASHI Toshitaka

O-GlucNAcylation：Ａ Morphologic Regulator of Osteoblast Differentiation Reviewed

2024.12

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皮膚創傷治癒におけるⅩⅧ型コラーゲンの機能解析 Reviewed

張暁雯, 米澤朋子, 前場崇宏, 上野智規, 大野充昭, 佐々木隆子, 百田龍輔, 水野一乘, 服部俊治, 大橋俊孝

第47回日本分子生物学会年会要旨集   2024.11

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Inverse genetics tracing the differentiation pathway of human chondrocytes. International journal

H T Do, M Ono, Z Wang, W Kitagawa, A T Dang, T Yonezawa, T Kuboki, T Oohashi, S Kubota

Osteoarthritis and cartilage   32 ( 11 )   1419 - 1432   2024.11

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OBJECTIVE: Mammalian somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) via the forced expression of Yamanaka reprogramming factors. However, only a limited population of the cells that pass through a particular pathway can metamorphose into iPSCs, while the others do not. This study aimed to clarify the pathways that chondrocytes follow during the reprogramming process. DESIGN: The fate of human articular chondrocytes under reprogramming was investigated through a time-coursed single-cell transcriptomic analysis, which we termed an inverse genetic approach. The iPS interference technique was also employed to verify that chondrocytes inversely return to pluripotency following the proper differentiation pathway. RESULTS: We confirmed that human chondrocytes could be converted into cells with an iPSC phenotype. Moreover, it was clarified that a limited population that underwent the silencing of SOX9, a master gene for chondrogenesis, at a specific point during the proper transcriptome transition pathway, could eventually become iPSCs. Interestingly, the other cells, which failed to be reprogrammed, followed a distinct pathway toward cells with a surface zone chondrocyte phenotype. The critical involvement of cellular communication network factors (CCNs) in this process was indicated. The idea that chondrocytes, when reprogrammed into iPSCs, follow the differentiation pathway backward was supported by the successful iPS interference using SOX9. CONCLUSIONS: This inverse genetic strategy may be useful for seeking candidates for the master genes for the differentiation of various somatic cells. The utility of CCNs in articular cartilage regeneration is also supported.

DOI： 10.1016/j.joca.2024.06.009

PubMed

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How Bioinformatics Accelerates Breakthrough in;Biomedical Research;Case Studies

2024.10

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Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Lecture material (seminar, tutorial, course, lecture, etc.)  

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Bioinformatics Reveals the Potential Mechanisms Underlying the Co-occurrence of Anorexia Nervosa and Exercise Addiction Reviewed

2024.9

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中枢無髄神経におけるｍTORシグナル伝達経路の役割 Reviewed

宮﨑晴子, 昌子浩孝, 宮下哲典, 原範和, 山中智行, 宮崎育子, 浅沼幹人, 宮川剛, 池内, 貫名信行, 大橋俊孝

第49回岡山脳研究セミナー抄録集   2024.9

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Language：Japanese   Publishing type：Lecture material (seminar, tutorial, course, lecture, etc.)  

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食品容器から溶出するエポキシ樹脂BADGEへの妊娠期曝露による産仔脳発達異常と自閉症様行動 Reviewed

正井加織, 宮崎育子, 禅正和真, 富本佳奈, 宮﨑晴子, 大橋俊孝, 浅沼幹人

第49回岡山脳研究セミナー抄録集   2024.9

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R-Shiny を用いたインタラクティブなscRNA-seq データ解析 Reviewed

大野充昭, Wang Ziyi, 米澤朋子, 大橋俊孝, 窪木拓男

NGS EXPO 2024   2024.9

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Analysis of striatal medium spiny neuron specific Tsc1 knockout mice Reviewed

2024.7

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Investigation of ECM remodeling in human aortic valve stenosis

2024.6

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Critical role of CCN family members in articular chondrocyte differentiation suggested by an inverse genetic approach Reviewed

Satoshi Kubota, Hang Thuy Do, Ziyi Wang, Wakana Kitagawa, Anh Tuan Dang, Tomoko Yonezawa, Takuo Kuboki, Mitsuaki Ono, Toshitaka Oohashi

12th International Workshop on the CCN Family of Genes   2024.6

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Assessment of perineuronal nets and parvalbumin interneurons in the brain of activity-based anorexia mouse model Reviewed

2024.6

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Understanding the mechanism of delayed long bone wou;healing in;aged;mice a;he single-cell level

2024.6

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Exploration of the origin of CAR cells, one of the bone marrow-derived mesenchymal stem cells, using scRNA-seq

2024.5

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Inverse genetics to trace the differen to trace the differentiation pathway of human chondrocytes retrospectively Reviewed

Hang Thuy Do, Mitsuaki Ono, Ziyi Wang, Wakana Kitagawa, Anh Tuan Dang, Tomoko Yonezawa, Takuo Kuboki, Toshitaka Oohashi, Satoshi Kubota

2024.3

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The difference between longbone and jawbone at single-cell resolution

DANG TA, ONO M., TOSA I., KITAGAWA W., WANG Z., YONEZAWA T., DO TH, ISHIBASHI K., KUBOKI T., OOHASHI T.

2024.3

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Filamin A Mediated Embryonic Palate Fusion: A Bioinformatics-oriented Study

Satoru Hayano, Ziyi Wang, Yao Weng, Xindi Mu, Mitsuaki Ono, Toshitaka Oohashi, Hiroshi Kamioka

2024 IADR/AADOCR/CADR General Session & Exhibition   2024.3

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Enhancing Vertical Bone Regeneration with Fibrin-Infused E-rhBMP-2

Kun Zhao, Mitsuaki Ono, Xindi Mu, Ziyi Wang, Tomoko Yonezawa, Takuo Kuboki, Toshitaka Oohashi

2024 IADR/AADOCR/CADR General Session & Exhibition   2024.3

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Retrospective re-discovery of the transcription factor that controls chondrocyte differentiation

DO THUY HANG, ONO MITSUAKI, WANG ZIYI, KITAGAWA WAKANA, DANG TUAN ANH, YONEZAWA TOMOKO, OOHASHI TOSHITAKA, KUBOKI TAKUO, KUBOTA SATOSHI

2024 IADR/AADOCR/CADR General Session & Exhibition   2024.3

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The Roles of BMP-2 on Modulating Oral Mucosal Keratinization

Xindi Mu, Mitsuaki Ono, Ha Thi Thu Nguyen, Ziyi Wang, Kun Zhao, Tomoko Yonezawa, Takuo Kuboki, Toshitaka Oohashi

2024 IADR/AADOCR/CADR General Session & Exhibition   2024.3

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