Faculty Profiles - MA Jian Feng

MISC - MA Jian Feng

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11-17 オオムギHvAACT1の5-UTRの挿入配列の解析 : イネによる検証(11.植物の有害元素,2010年度北海道大会)

横正健剛, 藤井美帆, 山地直樹, 馬建鋒

日本土壌肥料学会講演要旨集 56 ( 0 ) 2010

9-3 イネケイ酸輸送体遺伝子Lsi1を過剰発現したシロイヌナズナの解析(9.植物の多量栄養素,2010年度北海道大会)

三谷奈見季, 山地直樹, 馬建鋒

日本土壌肥料学会講演要旨集 56 ( 0 ) 2010

Isolation and characterization of two MATE genes in rye

Yokosho Kengo, Yamaji Naoki, Ma Jian Feng

Plant and Cell Physiology Supplement 2010 ( 0 ) 679 - 679 2010

Publisher：The Japanese Society of Plant Physiologists

Multidrug and toxic compound extrusion (MATE) proteins are widely present in bacteria, fungi, plants and mammals. Recent studies have showed that a group of plant MATE genes encodes citrate transporter, which are involved in the Al detoxification or Fe translocation from the roots to the shoots. In this study we isolated two homologous genes (ScFRDL1 and ScFRDL2) of this-type MATE in rye. ScFRDL1 shared 94 % identity with barley Al-induced citrate transporter HvAACT1 and ScFRDL2 shared 80 % identity with rice Al-induced gene OsFRDL2. Both genes were expressed in the roots, however, they showed different expression pattern. The expression of ScFRDL1 was unaffected by Al treatment, but induced by Fe-deficiency. By contrast, the expression of ScFRDL2 was induced by Al but not by Fe deficiency. Furthermore, the expression pattern of OsFRDL2 was consistent with citrate secretion pattern. Immunostaining showed that ScFRDL1 is localized at central cylinder in the basal roots. Taken together, our results suggest that ScFRDL1 is involved in efflux of citrate into the xylem for Fe translocation from root to shoot, while ScFRDL2 is involved in Al-activated citrate secretion in rye.

DOI： 10.14841/jspp.2010.0.0679.0

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植物のケイ酸吸収能力の差が新鮮火山灰のアルミニウム富化に及ぼす影響について

福永祥子, 阿江教治, 鈴木武志, 馬建鋒, 間藤徹, 藤嶽暢英

日本土壌肥料学会講演要旨集 55 26 2009.9

11-5 A rice Nramp gene is involved in Al tolerance

Xia Jixing

Abstracts of the meeting, the Society of the Science of Soil and Manure ( 55 ) 90 - 90 2009.9

11-10 Cloning and functional analysis of a rice gene involved in the tolerance to multiple metal toxicity

Abstracts of the meeting, the Society of the Science of Soil and Manure ( 55 ) 92 - 92 2009.9

Iron Acquisition Mechanism of Graminaceous Plants by Mugineic Acids

Murata Yoshiko, Namba Kosuke, Ma Jian Feng, Sugase Kenji, Horikawa Manabu, Iwashita Takashi, Kusumoto Shoichi

Symposium on the Chemistry of Natural Products, symposium papers ( 51 ) 103 - 108 2009.9

Language：Japanese Publisher：Symposium on the chemistry of natural products

Iron is an essential element for plant growth. Gramineous plants have generally developed a distinct strategy to efficiently acquire insoluble iron, which is characterized by the synthesis and secretion of an iron-chelating substance, phytosiderophore (PS) such as mugineic acid (MA), and by a specific uptake system for iron(III)-MAs complexes. In this study, we have identified a gene specifically encoding an iron(III)-MAS transporter (HvYS1) in barley. This gene as well as the encoded protein is specifically expressed in ghe epidermal cells of the roots and gene expression is greatly enhanced under iron-deficient conditions. The localization and substrate specificity of HvYS1 indicate that it is a specific transporter in barley roots. In contrast, ZmYS1, reported as iron-MAs transporter from maize, possesses broad substrate specificity despite a high homology with HvYS1. By assessing the transport activity of a series of HvYS1-ZmYS1 chimeras, we revealed that the outer membrane loop between the sixth and seenth transmembrane regions is essential for the substrate specificity. Circular dichroism spectra revealed that a synthetic peptide corresponding to the loop of HvYS1 forms an α-helix in solution, whereas that of ZmYS1 is flexible. We disclosed that the structural difference at this particular loop determines the substrate specificity of the HvYS1 transporter. We also achieved an efficient short-step synthesis of MA and 2'-deoxymugineic acid (DMA). Our new synthetic method enabled a sufficient supply of these compounds that are essential for further biological studies.

DOI： 10.24496/tennenyuki.51.0_103

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オオムギ由来排出型ケイ酸輸送体HvLsi2の更なる機能解析

三谷奈見季, 山地直樹, 千葉由佳子, 馬建鋒

日本植物生理学会年会要旨集 50th 2009

ケイ酸輸送体Lsi6によるイネ生殖生長期のケイ酸分配

山地直樹, 馬建鋒

日本植物生理学会年会要旨集 50th 2009

異なるイネ品種におけるカドミウム集積能の比較

小山絵美, 上野大勢, 馬建鋒

日本土壌肥料学会講演要旨集 55 2009

ゲノム育種による効率的品種育成技術の開発-QTL遺伝子解析の推進-3 環境ストレス耐性に関与する遺伝子の解析(6)イネアルミニウム耐性遺伝子の単離

馬建鋒

農林水産省農林水産技術会議事務局研究成果 ( 473 ) 2009

アルミニウムストレス耐性の分子機構

馬建鋒

日本土壌肥料学会講演要旨集 55 2009

イネにおけるケイ素と細胞壁多糖との相互作用解析

山本剛史, 古川純, 中村敦子, 古西智之, 石井忠, 岩井宏暁, 馬建鋒, 佐藤忍

日本植物学会大会研究発表記録 73rd 2009

44Kマイクロアレイによるイネアルミニウム応答遺伝子の網羅的解析

筒井友和, 黄朝鋒, 山地直樹, 長村吉晃, 馬建鋒

日本植物生理学会年会要旨集 50th 2009

カドミウム超集積植物Thlaspi caerulescens(ecotype,Ganges)由来のP-type ATPase HMA3の単離と機能解析

上野大勢, 山地直樹, 馬建鋒

日本植物生理学会年会要旨集 50th 2009

オオムギアルミニウム活性型クエン酸トランスポーター遺伝子(HvAACT1)の発現調節機構に関する研究

藤井美帆, 山地直樹, 佐藤和広, 馬建鋒

日本植物生理学会年会要旨集 50th 2009

有用遺伝子活用のための植物(イネ)・動物ゲノム研究-イネ・ゲノムの重要形質関連遺伝子の機能解明-第1章おもな個別研究課題の成果 5 不良環境に強い遺伝子の解明 2)ミネラルの吸収や利用を司る遺伝子の単離と応用

間藤徹, 西澤直子, 馬建鋒, 藤原徹

農林水産省農林水産技術会議事務局研究成果 ( 475 ) 2009

Cd超集積植物Thlaspi caerulescens(ecotype,Ganges)由来のP-type ATPase HMA3の機能解析

上野大勢, 山地直樹, 馬建鋒

日本土壌肥料学会講演要旨集 55 2009

イネのケイ素分配の分子機構(I)

山地直樹, 馬建鋒

日本土壌肥料学会講演要旨集 55 2009

Functional analysis of OsFRDL4, a citrate transporter involved in Al tolerance in rice

Yokosho Kengo, Yamaji Naoki, Ma Jian Feng

Plant and Cell Physiology Supplement 2009 ( 0 ) 436 - 436 2009

Publisher：The Japanese Society of Plant Physiologists

We recently characterized two MATE genes, HvAACT1 and OsFRDL1, which both are citrate transporter in barley and rice. HvAACT1 is involved in the Al-induced citrate secretion, while OsFRDL1 is involved in Fe translocation from the roots to the shoots. Here we report the function of a homolog of OsFRDL1, OsFRDL4, in rice. We first compared the phenotype between the wild-type and a Tos17 insertion line. Knockout of this gene did not cause chlorosis and decreased concentration of Fe and citrate in the xylem sap, but resulted in decreased Al-induced secretion of citrate from the roots. The protein encoded by this gene was able to transport citrate out of cell. All our results indicate that OsFRDL4 is a citrate transporter, which is involved in the Al-induced citrate secretion in rice. However, different from HvAACT1, which expression is not induced by Al, the expression of OsFRDL4 is greatly induced by Al.

DOI： 10.14841/jspp.2009.0.0436.0

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