2021/04/08 更新

写真a

マ ケンボウ
馬 建鋒
MA Jian Feng
所属
資源植物科学研究所 教授
職名
教授
外部リンク

学位

  • 農学博士 ( 京都大学 )

研究キーワード

  • Plant nutrition

  • 植物栄養学

研究分野

  • ライフサイエンス / 植物栄養学、土壌学

学歴

  • 京都大学   Graduate School, Division of Agriculture  

    - 1991年

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  • 京都大学    

    - 1991年

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    国名: 日本国

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  • 南京農業大学   土壌及び農業化学部   農業化学

    - 1984年

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    国名: 中華人民共和国

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経歴

  • - 岡山大学資源植物科学研究所 教授

    2005年

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  • - Professor,Institute of Plant Science and Resources,Okayama University

    2005年

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所属学協会

  • 日本植物生理学会

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  • 日本根の研究会

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  • 国際低pH植物土壌相互作用シンポジウム

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  • 日本土壌肥料学会

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  • International Symposium on Plant-Soil Interactions at Low pH

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  • 国際植物栄養会議

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  • International Plant Nutrition Colloquium

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委員歴

  • 国際低pH植物土壌相互作用シンポジウム   評議員  

    2015年   

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    団体区分:学協会

    国際低pH植物土壌相互作用シンポジウム

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  • International Symposium on Plant-Soil Interactions at Low pH   Committee member  

    2015年   

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    団体区分:学協会

    International Symposium on Plant-Soil Interactions at Low pH

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  • International Plant Nutrition Colloquium   Council member  

    2013年   

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    団体区分:学協会

    International Plant Nutrition Colloquium

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  • 国際植物栄養会議   評議員  

    2013年   

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    団体区分:学協会

    国際植物栄養会議

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  • 日本土壌肥料学会   代議員  

    2008年   

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    団体区分:学協会

    日本土壌肥料学会

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  • 日本植物生理学会   評議員  

    2008年   

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    団体区分:学協会

    日本植物生理学会

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論文

  • Transport of cadmium from soil to grain in cereal crops: A review

    Jian Feng Ma, Ren Fang Shen, Ji Feng Shao

    PEDOSPHERE31 ( 1 ) 3 - 10   2021年2月

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    記述言語:英語   出版者・発行元:SCIENCE PRESS  

    Due to rapid urbanization and industrialization, many soils for crop production are contaminated by cadmium (Cd), a heavy metal highly toxic to many organisms. Cereal crops such as rice, wheat, maize, and barley are the primary dietary source of Cd for humans, and reducing Cd transfer from soil to their grains is therefore an important issue for food safety. During the last decade, great progress has been made in elucidating the molecular mechanisms of Cd transport, particularly in rice. Inter- and intraspecific variations in Cd accumulation have been observed in cereal crops. Transporters for Cd have been identified in rice and other cereal crops using genotypic differences in Cd accumulation and mutant approaches. These transporters belong to different transporter families and are involved in the uptake, vacuolar sequestration, root-to-shoot translocation, and distribution of Cd. Attempts have been made to reduce Cd accumulation in grains by manipulating these transporters through overexpression or knockout of the transporter genes, as well as through marker-assisted selection breeding based on genotypic differences in Cd accumulation in the grains. In this review, we describe recent progress on molecular mechanisms of Cd accumulation in cereal crops and compare different molecular strategies for minimizing Cd accumulation in grains.

    DOI: 10.1016/S1002-0160(20)60015-7

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  • A transporter for delivering zinc to the developing tiller bud and panicle in rice

    Shuai Mu, Naoki Yamaji, Akimasa Sasaki, Le Luo, Binbin Du, Jing Che, Huichao Shi, Haoqiang Zhao, Sheng Huang, Fenglin Deng, Zhenguo Shen, Mary Lou Guerinot, Luqing Zheng, Jian Feng Ma

    PLANT JOURNAL105 ( 3 ) 786 - 799   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Tiller number is one of the most important agronomic traits that determine rice (Oryza sativa) yield. Active growth of tiller bud (TB) requires high amount of mineral nutrients; however, the mechanism underlying the distribution of mineral nutrients to TB with low transpiration is unknown. Here, we found that the distribution of Zn to TB is mediated by OsZIP4, one of the ZIP (ZRT, IRT-like protein) family members. The expression of OsZIP4 was highly detected in TB and nodes, and was induced by Zn deficiency. Immunostaining analysis revealed that OsZIP4 was mainly expressed in phloem of diffuse vascular bundles in the nodes and the axillary meristem. The mutation of OsZIP4 did not affect the total Zn uptake, but altered Zn distribution; less Zn was delivered to TB and new leaf, but more Zn was retained in the basal stems at the vegetative growth stage. Bioimaging analysis showed that the mutant aberrantly accumulated Zn in enlarged and transit vascular bundles of the basal node, whereas in wild-type high accumulation of Zn was observed in the meristem part. At the reproductive stage, mutation of OsZIP4 resulted in delayed panicle development, which is associated with decreased Zn distribution to the panicles. Collectively, OsZIP4 is involved in transporting Zn to the phloem of diffuse vascular bundles in the nodes for subsequent distribution to TBs and other developing tissues. It also plays a role in transporting Zn to meristem cells in the TBs.

    DOI: 10.1111/tpj.15073

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  • Fine regulation system for distribution of boron to different tissues in rice

    Ji Feng Shao, Naoki Yamaji, Sheng Huang, Jian Feng Ma

    NEW PHYTOLOGIST   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Boron (B) is essential for growth and development, with the B requirement differing depending on the particular organs and tissues, but the molecular mechanisms underlying the preferential distribution of B to different tissues are poorly understood.We investigated the role of a rice gene (OsBOR1) encoding a B efflux transporter in the distribution of B to different tissues under different B supplies.OsBOR1 was highly expressed in the nodes at all growth stages. The OsBOR1 protein shows polar localization at the distal side of bundle sheath cells in nodes and xylem parenchyma cells of elongating leaf sheath, but in the mature leaf sheath and blade at the proximal side of bundle sheath cells. Furthermore, the expression of OsBOR1 was not affected by external B fluctuations, but the OsBOR1 protein was gradually degraded in response to high B. Knockout of this gene altered B distribution, decreasing the distribution of B to new leaves and panicles but increasing B distribution to old leaves.These results indicate that OsBOR1 expressed in nodes and leaf sheath is involved in the preferential distribution of B to different tissues in rice. Furthermore, the OsBOR1 undergoes degradation in response to high B for fine regulation of B distribution to different tissues.

    DOI: 10.1111/nph.17169

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  • Role of a vacuolar iron transporter OsVIT2 in the distribution of iron to rice grains

    Jing Che, Naoki Yamaji, Jian Feng Ma

    NEW PHYTOLOGIST   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Iron (Fe) from rice grains is an important source of dietary intake; however, the molecular mechanisms responsible for loading of Fe to the grains are poorly understood.We functionally characterized a vacuolar iron transporter gene, OsVIT2 in terms of expression pattern, cellular localization, and mutant phenotypes.OsVIT2 was expressed in the parenchyma cell bridges of nodes, in the mestome sheath of leaf sheath and aleurone of the caryopsis. Mutation of OsVIT2 resulted in decreased Fe distribution to the leaf sheath, nodes, and aleurone, but increased Fe to the leaf blade and grains. Furthermore, Fe was heavily deposited in the parenchyma cell bridges, mestome sheath and aleurone in the wild-type rice, but this accumulation was decreased in the knockout lines. Conversely, heavier deposition of Fe was observed in the embryo and endosperm of the grains of knockout lines compared with the wild-type rice, resulting in increased Fe accumulation in the polished rice without yield penalty.These results indicate that OsVIT2 is involved in the distribution of Fe to the grains through sequestering Fe into vacuoles in mestome sheath, nodes, and aleurone layer and that knockout of this gene provides a potential way for Fe biofortification without yield penalty.

    DOI: 10.1111/nph.17219

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  • Linking transport system of silicon with its accumulation in different plant species

    Namiki Mitani-Ueno, Jian Feng Ma

    SOIL SCIENCE AND PLANT NUTRITION67 ( 1 ) 10 - 17   2021年1月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    Silicon (Si) is a beneficial element for plants, which helps to mitigate various biotic and abiotic stresses. Since the last review on Si published in this journal in 2004, great progress has been made in understanding transport system of Si in different plant species. The discovery of two different transporters for Si (Lsi1 and Lsi2) in rice led to intensive investigation of Si transporters in other plant species. Lsi1 belongs to the Nodulin 26-like intrinsic proteins (NIPs) subfamily in the aquaporin (AQP) family and functions as an influx transporter for Si. By contrast, Lsi2 belongs to the anion transporter superfamily and functions as an efflux transporter of Si. They are polarly localized at the distal and proximal sides, respectively, of both exodermis and endodermis of rice roots and are required for efficient uptake of Si. So far, homologs of Lsi1 and Lsi2 are identified not only in monocots, but also in dicots, which greatly differ in Si accumulation in the aboveground parts. However, the expression pattern, cell-type-specific expression, and polar localization of these transporters differ with plant species. In this review, we focus on recent progress in Si transporters identified in different plant species. We link these transporters with an accumulation of Si in different plant species in terms of expression pattern, cell-type-specific expression, polar localization of these transporters and propose three uptake systems of Si in different plant species. We also provide the perspectives toward a better understanding of Si transport system in different plant species and discuss its essentiality for plant growth.

    DOI: 10.1080/00380768.2020.1845972

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  • Cadmium transfer in contaminated soil-rice systems: Insights from solid-state speciation analysis and stable isotope fractionation

    Matthias Wiggenhauser, Anne-Marie Aucour, Sarah Bureau, Sylvain Campillo, Philippe Telouk, Marco Romani, Jian Feng Ma, Gautier Landrot, Geraldine Sarret

    ENVIRONMENTAL POLLUTION269   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Initial Cadmium (Cd) isotope fractionation studies in cereals ascribed the retention of Cd and its light isotopes to the binding of Cd to sulfur (S). To better understand the relation of Cd binding to S and Cd isotope fractionation in soils and plants, we combined isotope and XAS speciation analyses in soil-rice systems that were rich in Cd and S. The systems included distinct water management (flooded vs. non-flooded) and rice accessions with (excluder) and without (non-excluder) functional membrane transporter OsHMA3 that transports Cd into root vacuoles. Initially, 13% of Cd in the soil was bound to S. Through soil flooding, the proportion of Cd bound to S increased to 100%. Soil flooding enriched the rice plants towards heavy isotopes (delta Cd-114/110 = -0.37 to -0.39%) compared to the plants that grew on non-flooded soils (delta Cd-114/110 = -0.45 to -0.56%) suggesting that preferentially light Cd isotopes precipitated into Cd sulfides. Isotope compositions in CaCl2 root extracts indicated that the root surface contributed to the isotope shift between soil and plant during soil flooding. In rice roots, Cd was fully bound to S in all treatments. The roots in the excluder rice strongly retained Cd and its lights isotopes while heavy isotopes were transported to the shoots (Delta Cd-114/110(shoot-root) 0.16-0.19 parts per thousand). The non-excluder rice accumulated Cd in shoots and the apparent difference in isotope composition between roots and shoots was smaller than that of the excluder rice (Delta Cd-114/110(shoot-root) -0.02 to 0.08 parts per thousand). We ascribe the retention of light Cd isotopes in the roots of the excluder rice to the membrane transport of Cd by OsHMA3 and/or chelating Cd-S complexes in the vacuole. Cd-S was the major binding form in flooded soils and rice roots and partly contributed to the immobilization of Cd and its light isotopes in soil-rice systems. (C) 2020 The Author(s). Published by Elsevier Ltd.

    DOI: 10.1016/j.envpol.2020.115934

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  • Jian Feng Ma

    Jian Feng Ma

    NEW PHYTOLOGIST229 ( 2 ) 673 - 674   2021年1月

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    記述言語:英語   出版者・発行元:WILEY  

    DOI: 10.1111/nph.17066

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  • Two metallothionein genes highly expressed in rice nodes are involved in distribution of Zn to the grain

    Gui Jie Lei, Naoki Yamaji, Jian Feng Ma

    NEW PHYTOLOGIST229 ( 2 ) 1007 - 1020   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    A rice node is a hub for distribution of mineral elements; however, most genes highly expressed in the node have not been functionally characterized. Transcriptomic analysis of a rice node revealed that two metallothionein genes,OsMT2bandOsMT2c, were highly expressed in the node I. We functionally characterized these genes in terms of gene expression pattern, cellular and subcellular localization, phenotypic analysis of the single and double knockout mutants and metal-binding ability. BothOsMT2bandOsMT2cwere mainly and constitutively expressed in the phloem region of enlarged and diffuse vascular bundles in the nodes and of the anther. Knockout of eitherOsMT2borOsMT2cincreased zinc (Zn) accumulation in the nodes, but decreased Zn distribution to the panicle, resulting in decreased grain yield. A double mutant,osmt2bmt2c, showed further negative effects on the Zn distribution and grain yield. By contrast, knockout ofOsMT2bhad a small effect on copper (Cu) accumulation. Both OsMT2b and OsMT2c showed binding ability with Zn, whereas only OsMT2b showed binding ability with Cu in yeast. Our results suggest that bothOsMT2bandOsMT2cplay an important role mainly in the distribution of Zn to grain through chelation and subsequent transport of Zn in the phloem in rice.

    DOI: 10.1111/nph.16860

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  • Silicon suppresses zinc uptake through down-regulating zinc transporter gene in rice

    Sheng Huang, Jian Feng Ma

    PHYSIOLOGIA PLANTARUM170 ( 4 ) 580 - 591   2020年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    One of the beneficial effects of silicon (Si) is to improve nutrient imbalance including deficiency and excess of nutrients, however the molecular mechanisms underlying this effect are still poorly understood. In this study, we investigated the interaction between Si and zinc (Zn) in rice by using a mutant (lsi1) defective in Si uptake and its wild-type (WT, cv. Oochikara) at different Zn levels. High Zn inhibited the root elongation of both WT andlsi1mutant, but Si did not alleviate this inhibition in both lines. By contrast, Si supply decreased Zn concentration in both the roots and shoots of the WT, but not in thelsi1mutant. A short-term (24 h) labeling experiment with stable isotope(67)Zn showed that Si decreased(67)Zn uptake, but did not affect the root-to-shoot translocation and distribution ratio to different organs of(67)Zn in the WT. Furthermore, Si accumulated in the shoots, rather than Si in the external solution, is required for suppressing Zn uptake, but this was not caused by Si-decreased transpiration. A kinetic study showed that Si did not affect K(m)value of root Zn uptake, but decreased V(max)value in the WT. Analysis of genes related with Zn transport showed that among ZIP family genes, the expression of onlyOsZIP1implicated in Zn uptake, was down-regulated by Si in the WT, but not in thelsi1mutant. These results indicate that Si accumulated in the shoots suppresses the Zn uptake through down-regulating the transporter gene involved in Zn uptake in rice.

    DOI: 10.1111/ppl.13196

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  • The grapevine NIP2;1 aquaporin is a silicon channel

    Henrique Noronha, Angelica Silva, Namiki Mitani-Ueno, Carlos Conde, Farzana Sabir, Catarina Prista, Graca Soveral, Paul Isenring, Jian Feng Ma, Richard R. Belanger, Hernani Geros

    JOURNAL OF EXPERIMENTAL BOTANY71 ( 21 ) 6789 - 6798   2020年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Silicon (Si) supplementation has been shown to improve plant tolerance to different stresses, and its accumulation in the aerial organs is mediated by NIP2;1 aquaporins (Lsi channels) and Lsi2-type exporters in roots. In the present study, we tested the hypothesis that grapevine expresses a functional NIP2;1 that accounts for root Si uptake and, eventually, Si accumulation in leaves. Own-rooted grapevine cuttings of the cultivar Vinhao accumulated >0.2% Si (DW) in leaves when irrigated with 1.5 mM Si for 1 month, while Si was undetected in control leaves. Real-time PCR showed that VvNIP2;1 was highly expressed in roots and in green berries. The transient transformation of tobacco leaf epidermal cells mediated by Agrobacterium tumefaciens confirmed VvNIP2;1 localization at the plasma membrane. Transport experiments in oocytes showed that VvNIP2;1 mediates Si and arsenite uptake, whereas permeability studies revealed that VvNIP2;1 expressed in yeast is unable to transport water and glycerol. Si supplementation to pigmented grape cultured cells (cv. Gamay Freaux) had no impact on the total phenolic and anthocyanin content, or on the growth rate and VvNIP2;1 expression. Long-term experiments should help determine the extent of Si uptake over time and whether grapevine can benefit from Si fertilization.

    DOI: 10.1093/jxb/eraa294

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  • OsNRAMP1transporter contributes to cadmium and manganese uptake in rice

    Jia-Dong Chang, Sheng Huang, Naoki Yamaji, Wenwen Zhang, Jian Feng Ma, Fang-Jie Zhao

    PLANT CELL AND ENVIRONMENT43 ( 10 ) 2476 - 2491   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Rice is a major dietary source of the toxic metal, cadmium (Cd). Previous studies reported that the rice transporter, OsNRAMP1, (Natural resistance-associated macrophage protein 1) could transport iron (Fe), Cd and arsenic (As) in heterologous yeast assays. However, thein plantafunction of OsNRAMP1 remains unknown. Here, we showed that OsNRAMP1 was able to transport Cd and manganese (Mn) when expressed in yeast, but did not transport Fe or As.OsNRAMP1was mainly expressed in roots and leaves and encoded a plasma membrane-localized protein.OsNRAMP1expression was induced by Cd treatment and Fe deficiency. Immunostaining showed that OsNRAMP1 was localized in all root cells, except the central vasculature, and in leaf mesophyll cells. The knockout ofOsNRAMP1resulted in significant decreases in root uptake of Cd and Mn and their accumulation in rice shoots and grains, and increased sensitivity to Mn deficiency. The knockout ofOsNRAMP1had smaller effects on Cd and Mn uptake than knockout ofOsNRAMP5, while knockout of both genes resulted in large decreases in the uptake of the two metals. Taken together, OsNRAMP1 contributes significantly to the uptake of Mn and Cd in rice, and the functions of OsNRAMP1 and OsNRAMP5 are similar but not redundant.

    DOI: 10.1111/pce.13843

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  • Effect of ferritin on nitrogen fixation in Lotus japonicus nodules under various iron concentrations

    Yamikani Frank Chikoti, Supriadi, Mallika Duangkhet, Sirinapa Chungopast, Shigeyuki Tajima, Jian Feng Ma, Mika Nomura

    JOURNAL OF PLANT PHYSIOLOGY252   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH  

    In the nitrogen fixation process, iron plays a vital role by being part of many symbiotic proteins, such as nitrogenase and leghemoglobin, in an active symbiosis. Excess or insufficient iron in active nitrogen fixation negatively affects the entire process. In Lotus japonicus nodules, ferritin is expressed at the initial stages of nodule development and increases at the nodule senescence stage to mobilize iron release during that stage. In this study, we investigated the effects of overexpressing and suppressing ferritin on nitrogen fixation. Acetylene reduction activity revealed that nitrogen fixation is affected by the overexpression of ferritin at high iron concentrations, but at low iron concentrations, higher nitrogen fixation was observed in ferritin-suppressed plants. qRT-PCR data indicated that suppression of ferritin in nodules induces antioxidant genes, such as superoxide dismutase, dehydroascorbate reductase and ascorbate peroxidase, to detoxify reactive oxygen species. Our data suggest that suppressing ferritin in the nodules is effective for higher nitrogen fixation under iron deficient conditions. Overaccumulated ferritin in nodule is effective under the higher iron conditions, such as senescence state.

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  • Overexpression of the manganese/cadmium transporter OsNRAMP5 reduces cadmium accumulation in rice grain

    Jia-Dong Chang, Sheng Huang, Noriyuki Konishi, Peng Wang, Jie Chen, Xin-Yuan Huang, Jian Feng Ma, Fang-Jie Zhao

    JOURNAL OF EXPERIMENTAL BOTANY71 ( 18 ) 5705 - 5715   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Rice is a major dietary source of the toxic metal cadmium (Cd), and reducing its accumulation in the grain is therefore important for food safety. We selected two cultivars with contrasting Cd accumulation and generated transgenic lines overexpressing OsNRAMP5, which encodes a major influx transporter for manganese (Mn) and Cd. We used two different promoters to control the expression, namely OsActin1 and maize Ubiquitin. Overexpression of OsNRAMP5 increased Cd and Mn uptake into the roots, but markedly decreased Cd accumulation in the shoots, whilst having a relatively small effect on Mn accumulation in the shoots. The overexpressed OsNRAMP5 protein was localized to the plasma membrane of all cell types in the root tips and lateral root primordia without polarity. Synchrotron X-ray fluorescence mapping showed that the overexpression lines accumulated more Cd in the root tips and lateral root primordia compared with the wild-type. When grown in three Cd-contaminated paddy soils, overexpression of OsNRAMP5 decreased concentration of Cd in the grain by 49-94% compared with the wild type. OsNRAMP5-overexpression plants had decreased Cd translocation from roots to shoots as a result of disruption of its radial transport into the stele for xylem loading, demonstrating the effect of transporter localization and polarity on ion homeostasis.

    DOI: 10.1093/jxb/eraa287

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  • Breeding for low cadmium barley by introgression of a Sukkula-like transposable element

    Gui Jie Lei, Miho Fujii-Kashino, De Zhi Wu, Hiroshi Hisano, Daisuke Saisho, Fenglin Deng, Naoki Yamaji, Kazuhiro Sato, Fang-Jie Zhao, Jian Feng Ma

    NATURE FOOD1 ( 8 ) 489 - U17   2020年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGERNATURE  

    Barley is the fourth most produced cereal crop in the world and one of the major dietary sources of cadmium (Cd), which poses serious threats to human health. Here, we identify a gene that encodes a P-type heavy metal ATPase 3 (HvHMA3) responsible for grain Cd accumulation in barley. HvHMA3 from the high Cd barley variety Haruna Nijo in Japan and the low Cd variety BCS318 in Afghanistan shared 97% identity at the amino acid level. In addition, the HvHMA3 from both varieties showed similar transport activity for Cd and the same subcellular localization at the tonoplast. However, the expression of HvHMA3 was double in BCS318 than in Haruna Nijo. A 3.3-kilobase Sukkula-like transposable element was found to be inserted upstream of the gene in the low Cd variety, which functioned as a promoter and enhanced the expression of HvHMA3. Introgression of this insertion to an elite barley cultivar through backcrossing resulted in decreased Cd accumulation in the grain grown in Cd-contaminated soil without yield penalty. The decreased Cd accumulation resulting from the insertion was also found in some other barley landraces in the world. Our results indicate that insertion of the Sukkula-like transposable element plays an important role in upregulating HvHMA3 expression.The primary source of human exposure to the highly toxic heavy metal cadmium (Cd) is diet. This study identified a gene encoding a P-type heavy metal ATPase 3 (HvHMA3) that is responsible for Cd accumulation in barley grain. A Sukkula-like transposable element was found to play an important role in upregulating the expression of HvHMA3, thereby decreasing Cd accumulation in the grain.

    DOI: 10.1038/s43016-020-0130-x

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  • Diel magnesium fluctuations in chloroplasts contribute to photosynthesis in rice

    Jian Li, Kengo Yokosho, Sheng Liu, Hong Rui Cao, Naoki Yamaji, Xin Guang Zhu, Hong Liao, Jian Feng Ma, Zhi Chang Chen

    NATURE PLANTS6 ( 7 ) 848 - +   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Photosynthesis in plants responds to light fluctuations and displays circadian rhythms in various aspects. Here a new regulator of photosynthetic rhythms is identified in rice and shown to control diurnal magnesium fluctuations in the chloroplast. Further engineering attempts yield enhanced rice growth and photosynthesis.Photosynthesis provides food, fibre and fuel that support our society; understanding the mechanisms controlling dynamic changes in this process helps identify new options to improve photosynthesis. Photosynthesis shows diel changes, which have been largely attributed to external light/dark conditions, as well as internal gene expression and the post-translational modification of critical enzymes. Here we report diel fluctuations of magnesium (Mg) in rice (Oryza sativa) chloroplasts, which may function as a rhythm regulator contributing to the post-translational regulation of photosynthetic CO(2)assimilation in rice. We found that a chloroplast-localized Mg(2+)transporter gene,OsMGT3, which is rhythmically expressed in leaf mesophyll cells, partly modulates Mg fluctuations in rice chloroplasts. Knockout ofOsMGT3substantially reduced Mg(2+)uptake, as well as the amplitude of free Mg(2+)fluctuations in chloroplasts, which was closely associated with a decrease in ribulose 1,5-bisphosphate carboxylase activity in vivo and a consequent decline in the photosynthetic rate. In addition, the mesophyll-specific overexpression ofOsMGT3remarkably improved photosynthetic efficiency and growth performance in rice. Taken together, these observations demonstrate that OsMGT3-dependent diel Mg fluctuations in chloroplasts may contribute to Mg-dependent enzyme activities for photosynthesis over the daily cycle. Enhancing Mg(2+)input to chloroplasts could be a potential approach to improving photosynthetic efficiency in plants.

    DOI: 10.1038/s41477-020-0686-3

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  • The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions(1)([OPEN])

    Sheng Huang, Akimasa Sasaki, Naoki Yamaji, Haruka Okada, Namiki Mitani-Ueno, Jian Feng Ma

    PLANT PHYSIOLOGY183 ( 3 ) 1224 - 1234   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Zinc (Zn) is an important essential micronutrient for plants and humans; however, the exact transporter responsible for root zinc uptake from soil has not been identified. Here, we found that OsZIP9, a member of the ZRT-IRT-related protein, is involved in Zn uptake in rice (Oryza sativa) under Zn-limited conditions. OsZIP9 was mainly localized to the plasma membrane and showed transport activity for Zn in yeast (Saccharomyces cerevisiae). Expression pattern analysis showed thatOsZIP9was mainly expressed in the roots throughout all growth stages and its expression was upregulated by Zn-deficiency. Furthermore,OsZIP9was expressed in the exodermis and endodermis of root mature regions. For plants grown in a hydroponic solution with low Zn concentration, knockout ofOsZIP9significantly reduced plant growth, which was accompanied by decreased Zn concentrations in both the root and shoot. However, plant growth and Zn accumulation did not differ between knockout lines and wild-type rice under Zn-sufficient conditions. When grown in soil, Zn concentrations in the shoots and grains of knockout lines were decreased to half of wild-type rice, whereas the concentrations of other mineral nutrients were not altered. A short-term kinetic experiment with stable isotope(67)Zn showed that(67)Zn uptake in knockout lines was much lower than that in wild-type rice. Combined, these results indicate that OsZIP9 localized at the root exodermis and endodermis functions as an influx transporter of Zn and contributes to Zn uptake under Zn-limited conditions in rice.The zinc transporter OsZIP9, expressed at the exodermis and endodermis of root mature region, contributes to Zn uptake from soil in rice.

    DOI: 10.1104/pp.20.00125

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  • Plant Nutrition for Human Nutrition: Hints from Rice Research and Future Perspectives

    Sheng Huang, Peitong Wang, Naoki Yamaji, Jian Feng Ma

    MOLECULAR PLANT13 ( 6 ) 825 - 835   2020年6月

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    記述言語:英語   出版者・発行元:CELL PRESS  

    Both plants and humans require mineral elements for their healthy growth and development. Mineral elements in the soil are taken up by the plant roots and transported to the edible parts for human consumption through various different transporters. An ideal future crop for human health should be rich in essential mineral elements but with less toxic elements in the edible parts. However, due to the great difference in the numbers and amounts of mineral elements required between plants and humans, it is a challenge to balance plant growth and nutrient requirement for humans. In this article, we mainly focus on the transport system of mineral elements from soil to grain in rice, a staple food for half of the world's population, and discuss recent progress on the underlying genetic and physiological mechanisms. Examples are given for silicon, zinc, and iron essential/beneficial for both plants and humans, selenium and iodine only essential for humans, and toxic cadmium and arsenic for all organisms. Manipulation of some transporters for these elements, especially those localized in the node for allocation of mineral elements to the grain, has been successful in generating rice with higher density and bioavailability of essential elements but with less accumulation of toxic elements. We provide our perspectives toward breeding future crops for human health.

    DOI: 10.1016/j.molp.2020.05.007

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  • OASTL-A1 functions as a cytosolic cysteine synthase and affects arsenic tolerance in rice

    Chengcheng Wang, Lihua Zheng, Zhong Tang, Shengkai Sun, Jian Feng Ma, Xin-Yuan Huang, Fang-Jie Zhao

    JOURNAL OF EXPERIMENTAL BOTANY71 ( 12 ) 3678 - 3689   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Arsenic (As) contamination in paddy soil can cause phytotoxicity and elevated As accumulation in rice grains. Arsenic detoxification is closely linked to sulfur assimilation, but the genes involved have not been described in rice. In this study, we characterize the function of OASTL-A1, an O-acetylserine(thiol) lyase, in cysteine biosynthesis and detoxification of As in rice. Tissue expression analysis revealed that OsOASTL-A1 is mainly expressed in roots at the vegetative growth stage and in nodes at the reproductive stage. Furthermore, the expression of OsOASTL-A1 in roots was strongly induced by As exposure. Transgenic rice plants expressing pOsOASTL-A1::GUS (beta-glucuronidase) indicated that OsOASTL-A1 was strongly expressed in the outer cortex and the vascular cylinder in the root mature zone. Subcellular localization using OsOASTL-A1:eGFP (enhanced green fluorescent protein) fusion protein showed that OsOASTL-A1 was localized to the cytosol. In vivo and in vitro enzyme activity assays showed that OsOASTL-A1 possessed the O-acetylserine(thiol) lyase activity. Knockout of OsOASTL-A1 led to significantly lower levels of cysteine, glutathione, and phytochelatins in roots and increased sensitivity to arsenate stress. Furthermore, the osoastl-al knockout mutants reduced As accumulation in the roots, but increased As accumulation in shoots. We conclude that OsOASTL-A1 is the cytosolic O-acetylserine(thiol) lyase that plays an important role in non-protein thiol biosynthesis in roots for As detoxification.

    DOI: 10.1093/jxb/eraa113

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  • Plastic transport systems of rice for mineral elements in response to diverse soil environmental changes

    Peitong Wang, Naoki Yamaji, Komaki Inoue, Keiich Mochida, Jian Feng Ma

    NEW PHYTOLOGIST226 ( 1 ) 156 - 169   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Climate change will increase frequency of drought and flooding, which threaten global crop productivity and food security. Rice (Oryza sativa) is unique in that it is able to grow in both flooded and upland conditions, which have large differences in the concentrations and chemical forms of mineral elements available to plants. To comprehensively understand the mechanisms of rice for coping with different water status, we performed ionomics and transcriptomics analysis of the roots, nodes and leaves of rice grown in flooded and upland conditions. Focusing the analysis on genes encoding proteins involved in transport functions for mineral elements, it was found that, although rice plants maintained similar levels of each element in the shoots for optimal growth, different transporters for mineral elements were utilised for nitrogen, iron, copper and zinc to deal with different soil water conditions. For example, under flooded conditions, rice roots take up nitrogen using transporters for both ammonium (OsAMT1/2) and nitrate (OsNPF2.4, OsNRT1.1A and OsNRT2.3), whereas under upland conditions, nitrogen uptake is mediated by different nitrate transporters (OsNRT1.1B and OsNRT1.5A). This study shows that rice possesses plastic transport systems for mineral elements in response to different water conditions (upland and flooding).

    DOI: 10.1111/nph.16335

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  • Altered Root Structure Affects Both Expression and Cellular Localization of Transporters for Mineral Element Uptake in Rice

    En Yu, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY61 ( 3 ) 481 - 491   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    One of the most important roles of plant roots is to take up mineral elements for their growth. Although several genes involved in root growth have been identified, the association between root structure and mineral element uptake is less investigated. In this study, we isolated a rice mutant (dice1, defective in cell elongation 1) with short-root phenotype. This mutant was characterized by partial defect in the formation of root outer cell layers. Mapping of the responsible gene revealed that the short-root phenotype in the mutant was caused by a single-nucleotide substitution of a gene encoding a membrane-anchored endo-1,4-beta-glucanase (OsGlu3). The growth of both the roots and shoots was partially recovered with increasing strength of nutrient solution and glucose in the mutant. The mutant showed a decreased uptake (normalized by root dry weight) for Mg, Mn, Fe, Cu, Zn, Cd, As and Ge but increased uptake for K and Ca. The expression level of some transporter genes including OsLsi1 and OsLsi2 for Si uptake and OsNromp5 for Mn uptake was significantly decreased in the mutant compared with the wild-type (WT) rice. Furthermore, the cellular localization of OsLsi1 was altered; OsLsi1 localized at the root exodermis of the WT rice was changed to be localized to other cell layers of the mutant roots. However, this localization became normal in the presence of exogenous glucose in the mutant. Our results indicate that a normal root structure is required for maintaining the expression and localization of transporters involved in the mineral element uptake.

    DOI: 10.1093/pcp/pcz213

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  • Tomato roots have a functional silicon influx transporter but not a functional silicon efflux transporter

    Hao Sun, Yaoke Duan, Namiki Mitani-Ueno, Jing Che, Jianhua Jia, Jiaqi Liu, Jia Guo, Jian Feng Ma, Haijun Gong

    PLANT CELL AND ENVIRONMENT43 ( 3 ) 732 - 744   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Silicon (Si) accumulation in shoots differs greatly with plant species, but the molecular mechanisms for this interspecific difference are unknown. Here, we isolated homologous genes of rice Si influx (SlLsi1) and efflux (SlLsi2) transporter genes in tomato (Solanum lycopersicum L.) and functionally characterized these genes. SlLsi1 showed transport activity for Si when expressed in both rice lsi1 mutant and Xenopus laevis oocytes. SlLsi1 was constitutively expressed in the roots. Immunostaining showed that SlLsi1 was localized at the plasma membrane of both root tip and basal region without polarity. Furthermore, overexpression of SlLsi1 in tomato increased Si concentration in the roots and root cell sap but did not alter the Si concentration in the shoots. By contrast, two Lsi2-like proteins did not show efflux transport activity for Si in Xenopus oocytes. However, when functional CsLsi2 from cucumber was expressed in tomato, the Si uptake was significantly increased, resulting in higher Si accumulation in the leaves and enhanced tolerance of the leaves to water deficit and high temperature. Our results suggest that the low Si accumulation in tomato is attributed to the lack of functional Si efflux transporter Lsi2 required for active Si uptake although SlLsi1 is functional.

    DOI: 10.1111/pce.13679

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  • A transcription factor OsbHLH156 regulates Strategy II iron acquisition through localising IRO2 to the nucleus in rice

    Shoudong Wang, Lin Li, Yinghui Ying, Jin Wang, Ji Feng Shao, Naoki Yamaji, James Whelan, Jian Feng Ma, Huixia Shou

    NEW PHYTOLOGIST225 ( 3 ) 1247 - 1260   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Plants have evolved two strategies to acquire ferrous (Strategy I) or ferric (Strategy II) iron from soil. The iron-related bHLH transcription factor 2 (IRO2) has been identified as a key regulator of iron acquisition (Strategy II) in rice. However, its mode of action, subcellular localisation and binding partners are not clearly defined. Using RNA-seq analyses, we identified a novel bHLH-type transcription factor, OsbHLH156. The function of OsbHLH156 in Fe homeostasis was analysed by characterisation of the phenotypes, elemental content, transcriptome, interaction and subcellular localisation of OsbHLH156 and IRO2. OsbHLH156 is primarily expressed in the roots and transcript abundance is greatly increased by Fe deficiency. Loss of function of OsbHLH156 resulted in Fe-deficiency-induced chlorosis and reduced Fe concentration in the shoots under upland or Fe(III) supplied conditions. Transcriptome analyses revealed that the expression of most Fe-deficiency-responsive genes involved in Strategy II were not induced in the osbhlh156-1 mutant. Furthermore, OsbHLH156 was required for nuclear localisation of IRO2. We conclude that OsbHLH156 is required for a Strategy II uptake mechanism in rice, partnering with a previously identified 'master' regulator IRO2. Mechanistically it is required for the nuclear localisation of IRO2.

    DOI: 10.1111/nph.16232

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  • Functional evolution of nodulin 26-like intrinsic proteins: from bacterial arsenic detoxification to plant nutrient transport

    Benjamin Pommerrenig, Till A. Diehn, Nadine Bernhardt, Manuela D. Bienert, Namiki Mitani-Ueno, Jacqueline Fuge, Annett Bieber, Christoph Spitzer, Andrea Braeutigam, Jian Feng Ma, Francois Chaumont, Gerd P. Bienert

    NEW PHYTOLOGIST225 ( 3 ) 1383 - 1396   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Nodulin 26-like intrinsic proteins (NIPs) play essential roles in transporting the nutrients silicon and boron in seed plants, but the evolutionary origin of this transport function and the co-permeability to toxic arsenic remains enigmatic. Horizontal gene transfer of a yet uncharacterised bacterial AqpN-aquaporin group was the starting-point for plant NIP evolution. We combined intense sequence, phylogenetic and genetic context analyses and a mutational approach with various transport assays in oocytes and plants to resolve the transorganismal and functional evolution of bacterial and algal and terrestrial plant NIPs and to reveal their molecular transport specificity features. We discovered that aqpN genes are prevalently located in arsenic resistance operons of various prokaryotic phyla. We provided genetic and functional evidence that these proteins contribute to the arsenic detoxification machinery. We identified NIPs with the ancestral bacterial AqpN selectivity filter composition in algae, liverworts, moss, hornworts and ferns and demonstrated that these archetype plant NIPs and their prokaryotic progenitors are almost impermeable to water and silicon but transport arsenic and boron. With a mutational approach, we demonstrated that during evolution, ancestral NIP selectivity shifted to allow subfunctionalisations. Together, our data provided evidence that evolution converted bacterial arsenic efflux channels into essential seed plant nutrient transporters.

    DOI: 10.1111/nph.16217

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  • Vascular Cambium-Localized AtSPDT Mediates Xylem-to-Phloem Transfer of Phosphorus for Its Preferential Distribution in Arabidopsis

    Guangda Ding, Gui Jie Lei, Naoki Yamaji, Kengo Yokosho, Namiki Mitani-Ueno, Sheng Huang, Jian Feng Ma

    MOLECULAR PLANT13 ( 1 ) 99 - 111   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CELL PRESS  

    During plant growth and development mineral elements are preferentially delivered to different organs and tissues to meet the differential demand. It has been shown that the preferential distribution of mineral nutrients in gramineous plants is mediated by node-based transporters, but the mechanisms of preferential distribution in dicots are poorly understood. Here, we report a distinct mechanism for the preferential distribution of phosphorus (P) in Arabidopsis plants, revealed by detailed functional analysis of AtSPDT/AtSULTR3;4 (SULTR-like P Distribution Transporter), a homolog of rice OsSPDT. Like OsSPDT, AtSPDT is localized at the plasma membrane and showed proton-dependent transport activity for P. Interestingly, we found that AtSPDT is mainly expressed in the rosette basal region and leaf petiole, and its expression is up-regulated by P deficiency. Tissue-specific analysis showed that AtSPDT is mainly located in the vascular cambium of different organs, as well as in the parenchyma tissues of both xylem and phloem regions. Knockout of AtSPDT inhibited the growth of new leaves under low P due to decreased P distribution to those organs. The seed yields of the wild-type and atspdt mutant plants are similar, but the seeds of mutant plants contain - less P. These results indicate that AtSPDT localized in the vascular cambium is involved in preferential distribution of P to the developing tissues, through xylem-to-phloem transfer mainly at the rosette basal region and leaf petiole.

    DOI: 10.1016/j.molp.2019.10.002

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  • Silicon accumulation in rice plant aboveground biomass affects leaf carbon quality

    Jorg Schaller, Robin Heimes, Jian Feng Ma, Jean-Dominique Meunier, Ji Feng Shao, Miho Fujii-Kashino, Klaus Holger Knorr

    PLANT AND SOIL444 ( 1-2 ) 399 - 407   2019年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Background and aim Silicon is known to be able to substitute carbon in plant biomass, especially in cellulose, lignin and phenols. However, a more comprehensive picture regarding the effect of silicon accumulation on plant carbon quality (cellulose, lignin, phenol, wax, lipids, and free organic acids content) with regard to potential decomposability is still missing. Methods Two different rice varieties (French brown and red rice cultivars) were cultivated under five different soil silicon availabilities. After maturity we harvested the plants and analyzed them regarding carbon quality by FTIR spectroscopy and regarding plant silicon concentrations. Results Silicon accumulation was found to be dependent on silicon availability and on the specific rice cultivar. The lowering of carbon compounds content by silicon was found not to be restricted to cellulose, lignin and phenol. Silicon accumulation was able to decrease other carbon compounds such as fat, wax, lipids, and free organic acids, too. Conclusions Consequently, silicon is important for the carbon quality of silicon accumulating plants. Furthermore, silicon accumulation in plants is interfering with a large range of different carbon compounds potentially altering the leaf economic spectra, decomposability, and thus potentially interfering with the whole performance of ecosystems dominated by silicon accumulating plant species.

    DOI: 10.1007/s11104-019-04267-8

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  • The ABC transporter ABCG36 is required for cadmium tolerance in rice

    Shan Fu, Youshe Lu, Xiang Zhang, Guangzhe Yang, Dong Chao, Zhigang Wang, Mingxing Shi, Jiugeng Chen, Dai-Yin Chao, Rongbai Li, Jian Feng Ma, Jixing Xia

    JOURNAL OF EXPERIMENTAL BOTANY70 ( 20 ) 5909 - 5918   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Cadmium (Cd) is a highly toxic heavy metal in nature, which causes severe damage to plant growth. The molecular mechanisms for Cd detoxification are poorly understood. Here, we report that a G-type ATP-binding cassette transporter, OsABCG36, is involved in Cd tolerance in rice. OsABCG36 was expressed in both roots and shoots at a low level, but expression in the roots rather than the shoots was greatly up-regulated by a short exposure to Cd. A spatial expression analysis showed that Cd-induced expression of OsABCG36 was found in both the root tip and the mature root region. Transient expression of OsABCG36 in rice protoplast cells showed that it was localized to the plasma membrane. Immunostaining showed that OsABCG36 was localized in all root cells except the epidermal cells. Knockout of OsABCG36 resulted in increased Cd accumulation in root cell sap and enhanced Cd sensitivity, but did not affect tolerance to other metals including Al, Zn, Cu, and Pb. The concentration of Cd in the shoots was similar between the knockout lines and wild-type rice. Heterologous expression of OsABCG36 in yeast showed an efflux activity for Cd, but not for Zn. Taken together, our results indicate that OsABCG36 is not involved in Cd accumulation in the shoots, but is required for Cd tolerance by exporting Cd or Cd conjugates from the root cells in rice.

    DOI: 10.1093/jxb/erz335

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  • A Vacuolar Phytosiderophore Transporter Alters Iron and Zinc Accumulation in Polished Rice Grains

    Jing Che, Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    PLANT PHYSIOLOGY181 ( 1 ) 276 - 288   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Essential metals, such as iron (Fe) and zinc (Zn), in grains are important sources for seed germination and nutritional requirements, but the molecular mechanisms underlying their loading into grains are poorly understood. Recently, nodes in rice (Oryza sativa) were reported to play an important role in the preferential distribution of mineral elements to the grains. In this study, we functionally characterized a rice gene highly expressed in nodes, OsVMT (VACUOLAR MUGINEIC ACID TRANSPORTER), belonging to a major facilitator superfamily. OsVMT is highly expressed in the parenchyma cell bridges of node I, where Fe and Zn are highly deposited. The expression of OsVMT was induced by Fe deficiency in the roots but not in the shoot basal region and uppermost node. OsVMT localized to the tonoplast and showed efflux transport activity for 2'-deoxymugineic acid (DMA). At the vegetative stage, knockout of OsVMT resulted in decreased DMA but increased ferric Fe in the root cell sap. As a result, the concentration of DMA in the xylem sap increased but that of ferric Fe decreased in the xylem sap in the mutants. In the polished rice grain, the mutants accumulated 1.8- to 2.1-fold, 1.5- to 1.6-fold, and 1.4- to 1.5-fold higher Fe, Zn, and DMA, respectively, than the wild type. Taken together, our results indicate that OsVMT is involved in sequestering DMA into the vacuoles and that knockout of this gene enhances the accumulation of Fe and Zn in polished rice grains through DMA-increased solubilization of Fe and Zn deposited in the node.

    DOI: 10.1104/pp.19.00598

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  • Magnesium Deficiency Triggers SGR-Mediated Chlorophyll Degradation for Magnesium Remobilization

    Yu Yang Peng, Li Li Liao, Sheng Liu, Miao Miao Nie, Jian Li, Lu Dan Zhang, Jian Feng Ma, Zhi Chang Chen

    PLANT PHYSIOLOGY181 ( 1 ) 262 - 275   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Magnesium (Mg) is a relatively mobile element that is remobilized in plants under Mg-limited conditions through transport from old to young tissues. However, the physiological and molecular mechanisms underlying Mg remobilization in plants remain poorly understood. In this study, we investigated Mg remobilization in rice (Oryza sativa) as facilitated through a Mg dechelatase gene involved in chlorophyll degradation, STAY-GREEN (OsSGR). We first observed that mid-aged leaves of rice are more susceptible to Mg deficiency. Expression of OsSGR was specifically upregulated by Mg deficiency, and the response was more pronounced in mid-aged leaves. Knockout of OsSGR exhibited the stay-green phenotype, which hindered the mobility of Mg from mid-aged leaves to young developing leaves. This decline in Mg mobility was associated with inhibited growth of developing leaves in mutants under Mg-limited conditions. Furthermore, Mg deficiency enhanced reactive oxygen species (ROS) generation in mid-aged leaves. ROS levels, particularly hydrogen peroxide, in turn, positively regulated OsSGR expression, probably through chloroplast-to-nucleus signaling, which triggers chlorophyll degradation to protect mid-aged leaves from photodamage. Taken together, these results show that OsSGR-mediated chlorophyll degradation contributes to not only internal remobilization of Mg from mid-aged leaves to developing leaves, but also photooxidative protection of mid-aged leaves under Mg-limited conditions. ROS appear to act as feedback regulators of OsSGR expression to precisely govern chlorophyll degradation in mid-aged leaves where Mg and photosynthetic capacities are relatively high.

    DOI: 10.1104/pp.19.00610

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  • Bioimaging of multiple elements by high‐resolution LA ‐ ICP ‐ MS reveals altered distribution of mineral elements in the nodes of rice mutants 査読

    Naoki Yamaji, Jian Feng Ma

    The Plant Journal99 ( 6 ) 1254 - 1263   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/tpj.14410

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  • The Soybean Sugar Transporter GmSWEET15 Mediates Sucrose Export from Endosperm to Early Embryo

    Shoudong Wang, Kengo Yokosho, Runze Guo, James Whelan, Yong-Ling Ruan, Jian Feng Ma, Huixia Shou

    PLANT PHYSIOLOGY180 ( 4 ) 2133 - 2141   2019年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Soybean (Glycine max) seed is primarily composed of a mature embryo that provides a major source of protein and oil for humans and other animals. Early in development, the tiny embryos grow rapidly and acquire large quantities of sugars from the liquid endosperm of developing seeds. An insufficient supply of nutrients from the endosperm to the embryo results in severe seed abortion and yield reduction. Hence, an understanding of the molecular basis and regulation of assimilate partitioning involved in early embryo development is important for improving soybean seed yield and quality. Here, we used expression profiling analysis to show that two paralogous sugar transporter genes from the SWEET (Sugars Will Eventually be Exported Transporter) family, GmSWEET15a and GmSWEET15b, were highly expressed in developing soybean seeds. In situ hybridization and quantitative real-time PCR showed that both genes were mainly expressed in the endosperm at the cotyledon stage. GmSWEET15b showed both efflux and influx activities for sucrose in Xenopus oocytes. In Arabidopsis (Arabidopsis thaliana), knockout of three AtSWEET alleles is required to see a defective, but not lethal, embryo phenotype, whereas knockout of both GmSWEET15 genes in soybean caused retarded embryo development and endosperm persistence, resulting in severe seed abortion. In addition, the embryo sugar content of the soybean knockout mutants was greatly reduced. These results demonstrate that the plasma membrane sugar transporter, GmSWEET15, is essential for embryo development in soybean by mediating Suc export from the endosperm to the embryo early in seed development.

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  • In defence of the selective transport and role of silicon in plants

    Devrim Coskun, Rupesh Deshmukh, Humira Sonah, James G. Menzies, Olivia Reynolds, Jian Feng Ma, Herbert J. Kronzucker, Richard R. Belanger

    NEW PHYTOLOGIST223 ( 2 ) 514 - 516   2019年7月

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    記述言語:英語   出版者・発行元:WILEY  

    DOI: 10.1111/nph.15764

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  • Producing cadmium-free Indica rice by overexpressing OsHMA3

    Chenni Lu, Lingxiao Zhang, Zhong Tang, Xin-Yuan Huang, Jian Feng Ma, Fang-Jie Zhao

    ENVIRONMENT INTERNATIONAL126   619 - 626   2019年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Background: Considerable proportions of rice grains produced in some areas in southern China contain high concentrations of cadmium (Cd), leading to unsafe levels of dietary Cd intake. Cultivars of Indica rice, widely grown in southern China, are particularly prone to high Cd accumulation in the grain. Effective methods are needed to decrease Cd accumulation in Indica rice.Methods: OsHMA3, encoding a tonoplast Cd transporter, was overexpressed in an elite Indica rice cultivar (Zhongjiazao 17) driven by CaMV 35S promoter. The effects on Cd translocation, accumulation and tolerance, as well as on the agronomic traits and micronutrient concentrations were evaluated.Results: OsHMA3 overexpression markedly decreased Cd translocation from roots to shoots and increased Cd tolerance. OsHMA3 overexpression decreased Cd concentrations in brown rice by 94-98%, to levels just above the detection limit, when rice plants were grown in two Cd-contaminated paddy soils. OsHMA3 overexpression generally had no significant effect on grain yield and the concentrations of the essential micronutrients including zinc, iron, copper and manganese in field trials.Conclusion: Overexpression of OsHMA3 is a highly effective method to reduce Cd accumulation in Indica rice, producing rice grains that were almost Cd free with little effect on grain yield or essential micronutrient concentrations.

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  • The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice

    Hongmei Cai, Sheng Huang, Jing Che, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY70 ( 10 ) 2717 - 2725   2019年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    In order to respond to fluctuating zinc (Zn) in the environment, plants must have a system to control Zn homeostasis. However, how plants maintain an appropriate level of Zn during their growth and development is still poorly understood. In this study, we found that OsHMA3, a tonoplast-localized transporter for Zn/Cd, plays an important role in Zn homeostasis in rice. Accessions with the functional allele of OsHMA3 showed greater tolerance to high Zn than those with the non-functional allele based on root elongation test. A 67Zn-labeling experiment showed that accessions with loss of function of OsHMA3 had lower Zn accumulation in the roots but similar concentrations in the shoots compared with functional OsHMA3 accessions. When exposed to Zn-free growing medium, the concentration in the root cell sap was rapidly decreased in accessions with functional OsHMA3, but less dramatic changes were observed in non-functional accessions. A mobility experiment showed that more Zn in the roots was translocated to the shoots in accessions with functional OsHMA3. Higher expression levels of OsZIP4, OsZIP5, OsZIP8, and OsZIP10 were found in the roots of accessions with functional OsHMA3 in response to Zn deficiency. Taken together, our results indicate that OsHMA3 plays an important role in rice roots in both Zn detoxification and storage by sequestration into the vacuoles, depending on Zn concentration in the environment.

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  • A nodule‐localized phosphate transporter Gm PT 7 plays an important role in enhancing symbiotic N 2 fixation and yield in soybean 査読

    Liyu Chen, Lu Qin, Lili Zhou, Xinxin Li, Zhichang Chen, Lili Sun, Wenfei Wang, Zhihao Lin, Jing Zhao, Naoki Yamaji, Jian Feng Ma, Mian Gu, Guohua Xu, Hong Liao

    New Phytologist221 ( 4 ) 2013 - 2025   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

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  • OsPHT1;3 Mediates Uptake, Translocation, and Remobilization of Phosphate under Extremely Low Phosphate Regimes 査読

    Ming Xing Chang, Mian Gu, Yu Wei Xia, Xiao Li Dai, Chang Rong Dai, Jun Zhang, Shi Chao Wang, Hong Ye Qu, Naoki Yamaji, Jian Feng Ma, Guo Hua Xu

    PLANT PHYSIOLOGY179 ( 2 ) 656 - 670   2019年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Plant roots rely on inorganic orthophosphate (Pi) transporters to acquire soluble Pi from soil solutions that exists at micromolar levels in natural ecosystems. Here, we functionally characterized a rice (Oryza sativa) Pi transporter, Os Phosphate Transporter1;3 (OsPHT1;3), that mediates Pi uptake, translocation, and remobilization. OsPHT1;3 was directly regulated by Os Phosphate Starvation Response-2 and, in response to Pi starvation, showed enhanced expression in young leaf blades and shoot basal regions and even more so in roots and old leaf blades. OsPHT1;3 was able to complement a yeast mutant strain defective in five Pi transporters and mediate Pi influx in Xenopus laevis oocytes. Overexpression of OsPHT1;3 led to increased Pi concentration both in roots and shoots. However, unlike that reported for other known OsPHT1 members that facilitate Pi uptake at relatively higher Pi levels, mutation of OsPHT1;3 impaired Pi uptake and root-to-shoot Pi translocation only when external Pi concentration was below 5 mu M. Moreover, in basal nodes, the expression of OsPHT1;3 was restricted to the phloem of regular vascular bundles and enlarged vascular bundles. An isotope labeling experiment with P-32 showed that ospht1;3 mutant lines were impaired in remobilization of Pi from source to sink leaves. Furthermore, overexpression and mutation of OsPHT1;3 led to reciprocal alteration in the expression of OsPHT1;2 and several other OsPHT1 genes. Yeast-two-hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays all demonstrated a physical interaction between OsPHT1;3 and OsPHT1;2. Taken together, our results indicate that OsPHT1;3 acts as a crucial factor for Pi acquisition, root-to-shoot Pi translocation, and redistribution of phosphorus in plants growing in environments with extremely low Pi levels.

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  • The controversies of silicon's role in plant biology

    Devrim Coskun, Rupesh Deshmukh, Humira Sonah, James G. Menzies, Olivia Reynolds, Jian Feng Ma, Herbert J. Kronzucker, Richard R. Belanger

    NEW PHYTOLOGIST221 ( 1 ) 67 - 85   2019年1月

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    記述言語:英語   出版者・発行元:WILEY  

    Silicon (Si) is not classified as an essential plant nutrient, and yet numerous reports have shown its beneficial effects in a variety of species and environmental circumstances. This has created much confusion in the scientific community with respect to its biological roles. Here, we link molecular and phenotypic data to better classify Si transport, and critically summarize the current state of understanding of the roles of Si in higher plants. We argue that much of the empirical evidence, in particular that derived from recent functional genomics, is at odds with many of the mechanistic assertions surrounding Si's role. In essence, these data do not support reports that Si affects a wide range of molecular-genetic, biochemical and physiological processes. A major reinterpretation of Si's role is therefore needed, which is critical to guide future studies and inform agricultural practice. We propose a working model, which we term the 'apoplastic obstruction hypothesis', which attempts to unify the various observations on Si's beneficial influences on plant growth and yield. This model argues for a fundamental role of Si as an extracellular prophylactic agent against biotic and abiotic stresses (as opposed to an active cellular agent), with important cascading effects on plant form and function.

    DOI: 10.1111/nph.15343

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  • Two Genes Encoding a Bacterial-Type ATP-Binding Cassette Transporter are Implicated in Aluminum Tolerance in Buckwheat

    Jing Che, Naoki Yamaji, Kengo Yokosho, Ren Fang Shen, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY59 ( 12 ) 2502 - 2511   2018年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Buckwheat (Fagopyrum esculentum Moench) shows high tolerance to aluminum (Al) toxicity, but the molecular mechanisms underlying its high Al tolerance are poorly understood. Here, we functionally characterized two genes (FeSTAR1 and FeSTAR2), which encode a nucleotide-binding domain and a membrane domain, respectively, of a bacterial-type ATP-binding cassette (ABC) transporter. The expression of FeSTAR1 and FeSTAR2 was induced by Al in both roots and leaves with higher expression in the roots. Spatial and tissue-specific expression analysis showed that the Al-induced expression of these two genes was found in both the root tips and basal root regions with higher expression in the root outer cell layers. The expression was neither induced by other metals including Cd and La nor by low pH and phosphorus-deficiency. FeSTAR1 and FeSTAR2 were present in a single copy in the genome, but the Al-induced transcript copy number of FeSTAR1 and FeSTAR2 was much higher than their homologous genes in rice and Arabidopsis. FeSTAR1 and FeSTAR2 form a complex when co-expressed in onion epidermal cells. Introduction of FeSTAR1 and FeSTAR2 into Arabidopsis mutants atstar1 and als3/atstar2, respectively, rescued the sensitivity of the mutants to Al. Taken together, our results indicate that FeSTAR1 and FeSTAR2 are involved in Al tolerance and that their high expression level may contribute to high Al tolerance in buckwheat.

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  • Engineering rice with lower grain arsenic

    Fenglin Deng, Naoki Yamaji, Jian Feng Ma, Sang-Kyu Lee, Jong-Seong Jeon, Enrico Martinoia, Youngsook Lee, Won-Yong Song

    PLANT BIOTECHNOLOGY JOURNAL16 ( 10 ) 1691 - 1699   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Arsenic (As) is a poisonous element that causes severe skin lesions and cancer in humans. Rice (Oryza sativa L.) is a major dietary source of As in humans who consume this cereal as a staple food. We hypothesized that increasing As vacuolar sequestration would inhibit its translocation into the grain and reduce the amount of As entering the food chain. We developed transgenic rice plants expressing two different vacuolar As sequestration genes, ScYCF1 and OsABCC1, under the control of the RCc3 promoter in the root cortical and internode phloem cells, along with a bacterial gamma-glutamylcysteine synthetase driven by the maize UBI promoter. The transgenic rice plants exhibited reduced root-to-shoot and internode-to-grain As translocation, resulting in a 70% reduction in As accumulation in the brown rice without jeopardizing agronomic traits. This technology could be used to reduce As intake, particularly in populations of South East Asia suffering from As toxicity and thereby improve human health.

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  • Retrotransposon Insertion and DNA Methylation Regulate Aluminum Tolerance in European Barley Accessions 査読 国際誌

    Miho Kashino-Fujii, Kengo Yokosho, Naoki Yamaji, Miki Yamane, Daisuke Saisho, Kazuhiro Sato, Jian Feng Ma

    PLANT PHYSIOLOGY178 ( 2 ) 716 - 727   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Aluminum (Al) toxicity is a major stress factor limiting crop productivity in acid soil. Although there is great genotypic variation in tolerance to Al toxicity, the underlying molecular mechanisms are poorly understood. Here, we report that, in barley (Hordeum vulgare), the fourth largest cereal crop produced in the world, both retrotransposon insertion and DNA methylation are involved in regulating differential Al tolerance. HvAACT1 is a major gene responsible for citrate secretion from the roots for external detoxification of Al. A multiretrotransposon-like (MRL) sequence insertion at least 15.3 kb in length was detected in the upstream genomic region of HvAACT1 that displayed promoter activity and significantly enhanced HvAACT1 expression, especially in the root tips of Al-tolerant accessions. Furthermore, in a number of accessions with low levels of HvAACT1 expression, this MRL insertion was present but highly methylated. Geographical analysis showed that accessions with this MRL insertion are distributed mainly in European areas with acid soils. Two wild barley accessions were found to possess this MRL insertion, but with a high degree of methylation. These results indicate that the MRL insertion and its degree of DNA methylation influence HvAACT1 expression and that demethylation of this MRL insertion, which facilitates adaptation to acid soils, occurred following barley domestication. Moreover, our results indicate that barley accessions in East Asia and Europe have developed independent but equivalent strategies to withstand Al toxicity in acid soils.

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  • OsYSL16 is Required for Preferential Cu Distribution to Floral Organs in Rice

    Chang Zhang, Wenhui Lu, Yang Yang, Zhenguo Shen, Jian Feng Ma, Luqing Zheng

    PLANT AND CELL PHYSIOLOGY59 ( 10 ) 2039 - 2051   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Deficiency of copper (Cu) causes low fertility in many plant species, but the molecular mechanisms underlying distribution of Cu to the floral organs are poorly understood. Here, we found that a member of yellow-stripe like (YSL) family, YSL16 encoding the Cu-nicotianamine (Cu-NA) transporter, was highly expressed in the rachilla, with less expression in the palea and lemma of rice (Oryza sativa). beta-Glucuronidase (GUS) staining of transgenic rice carrying the OsYSL16 promoter-GUS showed that OsYSL16 was mainly expressed in vascular bundles of the rachilla as well as the palea and lemma. Knockout of OsYSL16 resulted in decreased Cu distribution to the stamens, but increased distribution to the palea and lemma. A short-term (24 h) Cu-65 labeling experiment confirmed increased Cu concentration of palea and lemma in the mutant. Furthermore, we found that redistribution of Cu from the palea and lemma was impaired in the osysl16 mutant after exposure to Cu-free solution. The osysl16 mutant showed low pollen germination, but this was rescued by addition of Cu in the medium. Our results indicate that OsYSL16 expressed in the vascular bundles of the rachilla is important for preferential distribution of Cu to the stamens, while OsYSL16 in vascular bundles of the palea and lemma is involved in Cu redistribution under Cu-limited conditions in rice.

    DOI: 10.1093/pcp/pcy124

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  • Functional characterization of an aluminum (Al)-inducible transcription factor, ART2, revealed a different pathway for Al tolerance in rice 査読

    Jing Che, Tomokazu Tsutsui, Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    NEW PHYTOLOGIST220 ( 1 ) 209 - 218   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    High aluminum (Al) tolerance in rice (Oryza sativa) is controlled by a Cys2His2-type zinc finger transcription factor ART1 (Al resistance transcription factor1). There are five close homologs of ART1 in the rice genome, but the role of these homologs is unknown.We functionally characterized one of the ART1 homologs, ART2, in terms of tissue and spatial expression, subcellular localization, transcriptional activation activity, and phenotypic analysis of the knockout lines.ART2 was localized to the nucleus and showed a transcriptional activation potential in yeast. ART2 was mainly expressed in the roots, but the expression level was much lower than that of ART1. The ART2 expression was rapidly induced by Al in the roots of the wild-type rice, but not in art1 mutant. Knockout of ART2 resulted in increased sensitivity to Al toxicity, but did not alter sensitivity to different pH values. Expression profile analysis by RNA-sequencing showed that ART2 was not involved in activation of genes regulated by ART1; however, four genes seems to be regulated by ART2, which are implicated in Al tolerance.These results indicate that ART1 and ART2 regulate different pathways leading to Al tolerance, and ATR2 plays a supplementary role in Al tolerance in rice.

    DOI: 10.1111/nph.15252

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  • The Putative Peptide Gene FEP1 Regulates Iron Deficiency Response in Arabidopsis

    Takashi Hirayama, Gui Jie Lei, Naoki Yamaji, Naoki Nakagawa, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY59 ( 9 ) 1739 - 1752   2018年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Iron is an essential element for all organisms, and plants have developed sophisticated systems to acquire iron and maintain iron homeostasis. We found that an Arabidopsis thaliana ABA-hypersensitive mutant, aba hypersensitive germination2-1 (ahg2-1), that is known to be defective in mitochondrial mRNA regulation, had increased expression of iron deficiency response genes. The ahg2-1 mutant had lower heme levels than the wild type. Transcriptome data further revealed that novel genes encoding short polypeptides were highly expressed in this mutant. The expression of one of these genes, which we named FE-UPTAKE-INDUCING PEPTIDE 1 (FEP1), was induced under iron-deficient conditions and was observed in the vascular tissues of the leaves and roots, as well as in leaf mesophyll cells. Notably, deletion or insertion mutations of FEN exhibited impaired iron accumulation in shoots but normal iron levels in roots. Artificially induced expression of FEP1 was sufficient to induce iron deficiency response genes, such as basic HELIX- LOOP-HELIX 38 (bHLH38), bHLH39, IRON-REGULATED TRANSPORTERI (IRT1) and FERRIC REDUCTION OXIDASE2 (FRO2), and led to iron accumulation in planta. Further analysis confirmed that the encoded peptide, but not the FEP1 RNA, was responsible for this activity. Remarkably, the activation of bHLH39 by FEP1 was independent of FER-LIKE IRON DEFICIENCY INDUCED (FIT), a key transcription factor in the iron deficiency response. Taken together, our results indicate that FEP1 functions in iron homeostasis through a previously undescribed regulatory mechanism for iron acquisition in Arabidopsis.

    DOI: 10.1093/pcp/pcy145

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  • A member of cation diffusion facilitator family, MTP11, is required for manganese tolerance and high fertility in rice 査読

    Yuta Tsunemitsu, Mayuko Genga, Tomoyuki Okada, Naoki Yamaji, Jian Feng Ma, Akira Miyazaki, Shin-ichiro Kato, Kozo Iwasaki, Daisei Ueno

    PLANTA248 ( 1 ) 231 - 241   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Rice MTP11 is the trans-Golgi-localized transporter that is involved in Mn tolerance with MTP8.1, and it is required for normal fertility.Rice (Oryza sativa L.) is one of the most manganese (Mn)-tolerant species, and it is able to accumulate high levels of this metal in the leaves without showing toxic symptoms. The metal tolerance protein 8.1 (MTP8.1), a member of the Mn-cation diffusion facilitator (CDF) family, has been shown to play a central role in high Mn tolerance by sequestering Mn into vacuoles. Recently, rice MTP11 was identified as an Mn transporter that is localized to Golgi-associated compartments, but its exact role in Mn tolerance in planta has not yet been understood. Here, we investigated the role of MTP11 in rice Mn tolerance using knockout lines. Old leaves presented higher levels of constitutively expressed MTP11 than other tissues and MTP11 expression was also found in reproductive organs. Fused MTP11:green fluorescent protein was co-localized to trans-Golgi markers and differentiated from other Golgi-associated markers. Knockout of MTP11 in wild-type rice did not affect tolerance and accumulation of Mn and other heavy metals, but knockout in the mtp8.1 mutant showed exacerbated Mn sensitivity at the vegetative growth stage. Knockout of MTP11 alone resulted in decreased grain yield and fertility at the reproductive stage. Thus, MTP11 is a trans-Golgi localized transporter for Mn, which plays a role in Mn tolerance through intracellular Mn compartmentalization. It is also required for maintaining high fertility in rice.

    DOI: 10.1007/s00425-018-2890-1

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  • Decreasing arsenic accumulation in rice by overexpressing OsNIP1;1 and OsNIP3;3 through disrupting arsenite radial transport in roots

    Sheng-Kai Sun, Yi Chen, Jing Che, Noriyuki Konishi, Zhong Tang, Anthony J. Miller, Jian Feng Ma, Fang-Jie Zhao

    NEW PHYTOLOGIST219 ( 2 ) 641 - 653   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Rice is a major dietary source of the toxic metalloid arsenic. Reducing arsenic accumulation in rice grain is important for food safety.We generated transgenic rice overexpressing two aquaporin genes, OsNIP1;1 and OsNIP3;3, under the control of a maize ubiquitin promoter or the rice OsLsi1 promoter, and tested the effect on arsenite uptake and translocation.OsNIP1;1 and OsNIP3;3 were highly permeable to arsenite in Xenopus oocyte assays. Both transporters were localized at the plasma membrane. Knockout of either gene had little effect on arsenite uptake or translocation. Overexpression of OsNIP1;1 or OsNIP3;3 in rice did not affect arsenite uptake but decreased root-to-shoot translocation of arsenite and shoot arsenic concentration markedly. The overexpressed OsNIP1;1 and OsNIP3;3 proteins were localized in all root cells without polarity. Expression of OsNIP1;1 driven by the OsLsi1 promoter produced similar effects. When grown in two arsenic-contaminated paddy soils, overexpressing lines contained significantly lower arsenic concentration in rice grain than the wild-type without compromising plant growth or the accumulation of essential nutrients.Overexpression of OsNIP1;1 or OsNIP3;3 provides a route for arsenite to leak out of the stele, thus restricting arsenite loading into the xylem. This strategy is effective in reducing arsenic accumulation in rice grain.

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  • Transcription factor WRKY22 promotes aluminum tolerance via activation of OsFRDL4 expression and enhancement of citrate secretion in rice (Oryza sativa) 査読

    Ge Zi Li, Zhan Qi Wang, Kengo Yokosho, Bing Ding, Wei Fan, Qiao Qiao Gong, Gui Xin Li, Yun Rong Wu, Jian Li Yang, Jian Feng Ma, Shao Jian Zheng

    NEW PHYTOLOGIST219 ( 1 ) 149 - 162   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Whilst WRKY transcription factors are known to be involved in diverse plant responses to biotic stresses, their involvement in abiotic stress tolerance is poorly understood. OsFRDL4, encoding a citrate transporter, has been reported to be regulated by ALUMINUM (Al) RESISTANCE TRANSCRIPTION FACTOR 1 (ART1) in rice, but whether it is also regulated by other transcription factors is unknown.We define the role of OsWRKY22 in response to Al stress in rice by using mutation and transgenic complementation assays, and characterize the regulation of OsFRDL4 by OsWRKY22 via yeas one-hybrid, electrophoretic mobility shift assay and ChIP-quantitative PCR.We demonstrate that loss of OsWRKY22 function conferred by the oswrky22 T-DNA insertion allele causes enhanced sensitivity to Al stress, and a reduction in Al-induced citrate secretion. We next show that OsWRKY22 is localized in the nucleus, functions as a transcriptional activator and is able to bind to the promoter of OsFRDL4 via W-box elements. Finally, we find that both OsFRDL4 expression and Al-induced citrate secretion are significantly lower in art1 oswrky22 double mutants than in the respective single mutants.We conclude that OsWRKY22 promotes Al-induced increases in OsFRDL4 expression, thus enhancing Al-induced citrate secretion and Al tolerance in rice.

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  • Efficient and flexible uptake system for mineral elements in plants 査読

    Jing Che, Naoki Yamaji, Jian Feng Ma

    NEW PHYTOLOGIST219 ( 2 ) 513 - 517   2018年7月

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    記述言語:英語   出版者・発行元:WILEY  

    Mineral elements required for plant growth and development must first be taken up by the roots from soil. Plants have developed an efficient uptake system for the radial transport of mineral elements from soil to central stele through the allocation of various transporters at different root cells. These transporters are regulated at transcriptional, translational and/or post-translational level to cope with the fluctuation of mineral elements in soil. In this insight, we describe an efficient uptake system for mineral elements formed by influx and efflux transporters, regulatory mechanisms and polarity of these transporters, and sensing and signal pathways, in response to spatial and temporal changes of mineral elements in soil. An understanding of the mineral element uptake system in different plant species, and its regulatory network, will contribute to high and safe crop production under varying environments.

    DOI: 10.1111/nph.15140

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  • Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of the OsHMA2 promoter 査読

    Ji Feng Shao, Jixing Xia, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY69 ( 10 ) 2743 - 2752   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Reducing cadmium (Cd) accumulation in rice grain is an important issue for human health. The aim of this study was to manipulate both expression and tissue localization of OsHMA3, a tonoplast-localized Cd transporter, in the roots by expressing it under the control of the OsHMA2 promoter, which shows high expression in different organs including roots, nodes, and shoots. In two independent transgenic lines, the expression of OsHMA3 was significantly enhanced in all organs compared with non-transgenic rice. Furthermore, OsHMA3 protein was detected in the root pericycle cells and phloem region of both the diffuse vascular bundle and the enlarged vascular bundle of the nodes. At the vegetative stage, the Cd concentration in the shoots and xylem sap of the transgenic rice was significantly decreased, but that of the whole roots and root cell sap was increased. At the reproductive stage, the concentration of Cd, but not other essential metals, in the brown rice of transgenic lines was decreased to less than one-tenth that of the non-transgenic rice. These results indicate that expression of OsHMA3 under the control of the OsHMA2 promoter can effectively reduce Cd accumulation in rice grain through sequestering more Cd into the vacuoles of various tissues.

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  • Preferential Distribution of Boron to Developing Tissues Is Mediated by the Intrinsic Protein OsNIP3 査読

    Ji Feng Shao, Naoki Yamaji, Xin Wei Liu, Kengo Yokosho, Ren Fang Shen, Jian Feng Ma

    PLANT PHYSIOLOGY176 ( 2 ) 1739 - 1750   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Boron is especially required for the growth of meristem and reproductive organs, but the molecular mechanisms underlying the preferential distribution of B to these developing tissues are poorly understood. Here, we show evidence that a member of nodulin 26-like intrinsic protein (NIP), OsNIP3; 1, is involved in this preferential distribution in rice (Oryza sativa). OsNIP3; 1 was highly expressed in the nodes and its expression was up-regulated by B deficiency, but down-regulated by high B. OsNIP3; 1 was polarly localized at the xylem parenchyma cells of enlarged vascular bundles of nodes facing toward the xylem vessels. Furthermore, this protein was rapidly degraded within a few hours in response to high B. Knockout of this gene hardly affected the uptake and root-to-shoot translocation of B, but altered B distribution in different organs in the above-ground parts, decreased distribution of B to the new leaves, and increased distribution to the old leaves. These results indicate that OsNIP3; 1 located in the nodes is involved in the preferential distribution of B to the developing tissues by unloading B from the xylem in rice and that it is regulated at both transcriptional and protein level in response to external B level.

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  • OsNRT2.4 encodes a dual-affinity nitrate transporter and functions in nitrate-regulated root growth and nitrate distribution in rice 査読

    Jia Wei, Yi Zheng, Huimin Feng, Hongye Qu, Xiaorong Fan, Naoki Yamaji, Jian Feng Ma, Guohua Xu

    JOURNAL OF EXPERIMENTAL BOTANY69 ( 5 ) 1095 - 1107   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Plant NRT2 nitrate transporters commonly require a partner protein, NAR2, for transporting nitrate at low concentrations, but their role in plants is not well understood. In this study, we characterized the gene for one of these transporters in the rice genome, OsNRT2.4, in terms of its activity and roles in rice grown in environments with different N supply. In Xenopus oocytes, OsNRT2.4 alone without OsNAR2 co-expression facilitated nitrate uptake showing biphasic kinetics at a wide concentration range, with high- and low-affinity K-M values of 0.15 and 4 mM, respectively. OsNRT2.4 did not have nitrate efflux or IAA influx activity. In rice roots, OsNRT2.4 was expressed mainly in the base of lateral root primordia. Knockout of OsNRT2.4 decreased lateral root number and length, and the total N uptake per plant at both 0.25 and 2.5 mM NO3- levels. In the shoots, OsNRT2.4 was expressed mainly in vascular tissues, and its knockout decreased the growth and NO3--N distribution. Knockout of OsNRT2.4, however, did not affect rice growth and N uptake under conditions without N or with only NH4+ supply. We conclude that OsNRT2.4 functions as a dual-affinity nitrate transporter and is required for nitrate-regulated root and shoot growth of rice.

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  • T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress 査読

    Yuuka Oda, Natsuko I. Kobayashi, Keitaro Tanoi, Jian Feng Ma, Yukiko Itou, Maki Katsuhara, Takashi Itou, Tomoaki Horie

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES19 ( 1 )   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI  

    The high affinity K+ transporter 1;4 (HKT1;4) in rice (Oryza sativa), which shows Na+ selective transport with little K+ transport activity, has been suggested to be involved in reducing Na in leaves and stems under salt stress. However, detailed physiological roles of OsHKT1;4 remain unknown. Here, we have characterized a transfer DNA (T-DNA) insertion mutant line of rice, which overexpresses OsHKT1;4, owing to enhancer elements in the T-DNA, to gain an insight into the impact of OsHKT1;4 on salt tolerance of rice. The homozygous mutant (the O/E line) accumulated significantly lower concentrations of Na in young leaves, stems, and seeds than the sibling WT line under salt stress. Interestingly, however, the mutation rendered the O/E plants more salt sensitive than WT plants. Together with the evaluation of biomass of rice lines, rhizosphere acidification assays using a pH indicator bromocresol purple and (NaCl)-Na-22 tracer experiments have led to an assumption that roots of O/E plants suffered heavier damages from Na which excessively accumulated in the root due to increased activity of Na+ uptake and Na+ exclusion in the vasculature. Implications toward the application of the HKT1-mediated Na+ exclusion system to the breeding of salt tolerant crop cultivars will be discussed.

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  • Rice reduces Mn uptake in response to Mn stress 査読

    Yuta Tsunemitsu, Naoki Yamaji, Jian Feng Ma, Shin-ichiro Kato, Kozo Iwasaki, Daisei Ueno

    PLANT SIGNALING & BEHAVIOR13 ( 1 )   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS INC  

    Rice (Oryza sativa L) is one of the most Mn-tolerant crops that can grow in submerged paddy fields, where the Mn concentration in soil solution is very high due to reduction. Although a large part of Mn is transferred from the roots to the shoot in rice, the roots are constantly exposed to high Mn concentrations in submerged paddies. Thus, mechanisms for preventing Mn overaccumulation in the cytoplasm of root cells are necessary. Recently, we showed that two cation diffusion facilitators, MTP8.1 and MTP8.2, play a crucial role in Mn tolerance in rice roots by sequestering Mn in vacuoles. Moreover, we observed that disruption of MTP8.1 and MTP8.2 resulted in reduced Mn accumulation under excess Mn. In the present study, we examined the effects of disruption of MTP8.1 and MTP8.2 on Mn uptake and determined that this phenotype is caused by a rapid and significant reduction of Mn uptake in response to excess Mn. Previously, we showed that Mn export from root cells through MTP9 was promoted by high Mn. Together, these findings suggest that optimal Mn concentration in rice roots is maintained by reduced uptake, vacuolar sequestration, and extrusion by cation diffusion facilitators.

    DOI: 10.1080/15592324.2017.1422466

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  • Both retrotransposon insertion and demethylation regulate aluminum tolerance of European barley for postdomestication expansion to acid soil.

    Fujii-Kashino, M, Yamaji, N, Yamane, M, Saisho, D, Sato, K, Ma, J. F

    Plant Physiology   2018年

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  • Reducing phosphorus accumulation in rice grains with an impaired transporter in the node (vol 541, pg 92, 2017) 査読

    Naoki Yamaji, Yuma Takemoto, Takaaki Miyaji, Namiki Mitani-Ueno, Kaoru T. Yoshida, Jian Feng Ma

    NATURE541 ( 7635 ) 136 - 136   2017年1月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    DOI: 10.1038/nature21404

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  • A model of silicon dynamics in rice 査読

    Gen Sakurai, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Keisuke Ono, Jian Feng Ma

    Frontiers in Plant Science8   E31-E34   2017年

  • 植物栄養と数理モデルの接点ー数理モデルで植物栄養の仕組みを理解する

    馬建鋒, 小林高範, 山地直樹, 小林安文, 小山博之, 岩本明敏, 櫻井玄

    日本土壌肥料学会誌86   226 - 230   2015年

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    記述言語:日本語  

    DOI: 10.20710/dojo.86.3_232

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  • Study of alkaline lignin from Arundo donax Linn based on FT Raman spectroscopy

    Ting-Ting You, Jian-Feng Ma, Si-Qin Guo, Feng Xu

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY247   2014年3月

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    記述言語:英語   出版者・発行元:AMER CHEMICAL SOC  

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  • LjMATE1: A Citrate Transporter Responsible for Iron Supply to the Nodule Infection Zone of Lotus japonicas (vol 54, pg 585, 2013)

    Kojiro Takanashi, Kengo Yokosho, Kazuhiko Saeki, Akifumi Sugiyama, Shusei Sato, Satoshi Tabata, Jian Feng Ma, Kazufumi Yazaki

    PLANT AND CELL PHYSIOLOGY54 ( 10 ) 1749 - 1749   2013年10月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    DOI: 10.1093/pcp/pct118

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  • ABC transporter for Al tolerance 査読

    Chao-Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma

    The Plant Journal69 ( 5 ) 857 - 867   2012年

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    Chao-Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma, 2012, 'ABC transporter for Al tolerance', <i>The Plant Journal</i>, vol. 69, no. 5, pp. 857-867

    DOI: 10.1111/j.1365-313x.2011.04837.x

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  • Identification and application of genes related to toxic element accumulation in rice

    Ma Jian Feng

    GENES & GENETIC SYSTEMS86 ( 6 ) 399 - 399   2011年12月

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    記述言語:英語   出版者・発行元:GENETICS SOC JAPAN  

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  • Understanding how plants cope with acid soils

    Jian Feng Ma, Peter R. Ryan

    FUNCTIONAL PLANT BIOLOGY37 ( 4 ) III - VI   2010年

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    記述言語:英語   出版者・発行元:CSIRO PUBLISHING  

    Supplying food and fuel to meet the demands of a growing population is a challenge facing every society. Crop yields will need to increase significantly over the next 40 years to support an extra two billion people by 2050. Acid soils limit plant production around the world but especially in the tropical and sub-tropical latitudes where a large proportion of the population increases are expected to occur. Aluminium toxicity and phosphorus deficiency are two of the major stresses affecting plant growth on acid soils. How plants combat these stresses was a theme at the '7th International Symposium on Plant-Soil Interactions at Low pH', held in Guangzhou in 2009, and the focus of this issue of FPB. We provide an overview of recent progress in the area and introduce a selection of invited papers for this research front on acid soils.

    DOI: 10.1071/FPv37n4_FO

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  • A specific transporter for iron(III)-phytosiderophore in barley roots (vol 46, pg 563, 2006) 査読

    Murata Y, Ma J. F, Yamaji N, Ueno D, Nomoto K, Iwashita T

    Plant Journal61 ( 1 ) 188   2010年

  • Silicon Transporters in Higher Plants

    Jian Feng Ma

    MIPS AND THEIR ROLE IN THE EXCHANGE OF METALLOIDS679   99 - 110   2010年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG BERLIN  

    Silicon (Si) is the second most abundant element in the Earth's crust and exerts beneficial effects on plant growth and production by alleviating both biotic and abiotic stresses including diseases, pests, lodging, drought and nutrient imbalance. Silicon is taken up by the roots in the form of silicic acid, a noncharged molecule. Recently both influx (Lsi1) and efflux (Lsi2) transporters for silicic acid have been identified in gramineous plants including rice, barley and maize. Lsi1 and its homologs are influx Si transporters, which belong to a Nod26-like major intrinsic protein (NIP) subfamily in the aquaporin protein family. They are responsible for the transport of Si from the external solution to the root cells. On the other hand, Lsi2 and its homologs are efflux Si transporters, belonging to putative anion transporters and are responsible for the transport of Si out of the cells toward the xylem. All influx transporters show polar localization at the distal side. Among efflux transporters, Lsi2 in rice shows polar localization at the proximal side, but that in barley and maize does not show polar localization. The cell-specificity of localization of Si transporters and expression patterns are different between species. Rice Si transporters are also permeable to arsenite.

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  • Arsenic transport in plants

    Fangjie Zhao, Jian Feng Ma, Steve P. McGrath

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY153A ( 2 ) S187 - S187   2009年6月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    DOI: 10.1016/j.cbpa.2009.04.411

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  • Characterization of silicon permeability of NIP in gramineous plants 査読

    Namiki Mitani, Naoki Yamaji, Maki Katsuhara, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY48   S30 - S30   2007年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

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  • Gene expression and functional analysis of a candidate gene related to Al-activated citrate transporter in barley 査読

    Jun Furukawa, Naoki Yamaji, Hua Wang, Maki Katsuhara, Kazuhiro Sato, Kazuyoshi Takeda, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY48   S44 - S44   2007年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

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  • Functional analysis of a rice Si transporter homolog Lsi6. 査読

    Naoki Yamaji, Namiki Mitani, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY48   S30 - S30   2007年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    DOI: 10.14841/jspp.2007.0.054.0

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  • Further characterization of an Fe-phytosiderophore transporter gene ZmYS 1 in maize 査読

    Daisei Ueno, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY48   S29 - S29   2007年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

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  • イネの生産性・品質と栄養生理 査読

    牧野 周, 早川俊彦, 馬 建鋒, 安藤 豊, 近藤始彦, 寺尾富夫, 眞崎 聡, 金田吉弘, 前 忠彦, 林 浩昭, 大山卓爾

    日本土壌肥料学雑誌77   361 - 365   2006年6月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.20710/dojo.77.3_361

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▼全件表示

書籍等出版物

  • Signaling, Communication in Plants. Vol.24, Aluminum Stress Adaptation in Plants.

    Springer Link  2015年  ( ISBN:9783319199689

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  • Handbook of Plant Nutrition, Second Edition.

    CRC Press.  2015年  ( ISBN:9781439881972

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  • Encyclopedia of Metalloproteins.

    Springer.  2013年 

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  • Mineral Nutrition of Higher Plants. Third Edition.

    Elsevier.  2011年 

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  • Mineral Nutrition of Higher Plants. Third Edition.

    Elsevier.  2011年 

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  • Advances in Experimental Medicine and Biology. MIPs and Their Role in the Exchange of Metalloids.

    Springer.  2010年  ( ISBN:9781441963147

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  • 植物栄養学 第2版.

    文永堂出版.  2010年  ( ISBN:9784830041198

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  • Handbook of Biomineralization

    WILEY-VCH  2007年 

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  • Advances in Agronomy

    Academic Press.  2007年  ( ISBN:9780123742063

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  • イネの生産性・品質と栄養生理

    博友社  2006年 

     詳細を見る

  • 農学大辞典

    養賢堂  2004年 

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  • Physiological Plant Ecology

    Springer  2003年 

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  • Silicon Biomineralization

    Springer  2003年 

     詳細を見る

  • 植物栄養・肥料の事典

    朝倉書店  2002年 

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  • Encyclopedia of Soil Science

    Marcel Dekker, Inc. New York  2002年 

     詳細を見る

  • Soil, Fertilizer, and Plant Silicon Research in Japan

    Elsevier Science  2002年 

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▼全件表示

MISC

  • イネのART1と相互作用するタンパク質OsBBPIsの機能解析

    横正健剛, 陳志長, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集64   66   2018年8月

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    記述言語:日本語  

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  • Silicon accumulated in the shoots results in down-regulation of phosphorus transporter gene expression and decrease of phosphorus uptake in rice

    An Yong Hu, Jing Che, Ji Feng Shao, Kengo Yokosho, Xue Qiang Zhao, Ren Fang Shen, Jian Feng Ma

    PLANT AND SOIL423 ( 1-2 ) 317 - 325   2018年2月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Silicon (Si) as a beneficial element can improve nutrient imbalance, but the molecular mechanism for this effect is poorly understood. The objective of this study is to examine the mechanism underlying Si-induced decrease of phosphorus (P) uptake in rice (Oryza sativa) at adequate/high P supply.A rice mutant (lsi1) defective in Si uptake and its wild type (cv. Oochikara) were used. The P uptake was compared in the presence and absence of Si and the expression of Pi transporter genes was quantified.Si addition in the nutrient solution significantly decreased shoot P concentration and uptake in the WT, but not in lsi1 mutant at two P levels, adequate (90 mu M) and high (210 mu M). Neither the root-to-shoot translocation of P nor the P distribution in different organs was altered by Si in both WT and lsi1. Heterogeneous expression of Lsi1 in Xenopus oocyte did not show transport activity for Pi. The expression of Pi transporter genes (OsPT1, 2 and 8) in the roots was hardly affected by Si in both WT and lsi1, but that of OsPT6 was down-regulated by Si in the WT roots, but not in the lsi1 roots. Furthermore, a split root experiment showed that Si accumulated in the shoots suppressed the expression of OsPT6. In rice grown in paddy field, lsi1 showed higher P concentration in the straw, husk and brown rice than the WT.Si decreased P uptake through down-regulating the expression of P transporter gene, OsPT6 in rice and Si accumulated in the shoot is required for this down-regulation.

    DOI: 10.1007/s11104-017-3512-6

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  • Two MATE Transporters with Different Subcellular Localization are Involved in Al Tolerance in Buckwheat

    Gui Jie Lei, Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY58 ( 12 ) 2179 - 2189   2017年12月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Buckwheat (Fagopyrum esculentum) shows high tolerance to aluminum (Al) toxicity, but the molecular mechanisms responsible for this high Al tolerance are still poorly understood. Here, we investigated the involvement of two MATE (multi-drug and toxic compound extrusion) genes in Al tolerance. Both FeMATE1 and FeMATE2 showed efflux transport activity for citrate, but not for oxalate when expressed in Xenopus oocytes. A transient assay with buckwheat leaf protoplasts using green fluorescent protein (GFP) fusion showed that FeMATE1 was mainly localized to the plasma membrane, whereas FeMATE2 was localized to the trans-Golgi and Golgi. The expression of FeMATE1 was induced by Al only in the roots, but that of FeMATE2 was up-regulated in both the roots and leaves. Furthermore, the expression of both genes only responded to Al toxicity, but not to other stresses including low pH, cadmium (Cd) and lanthanum (La). Heterologous expression of FeMATE1 or FeMATE2 in the Arabidopsis mutant atmate partially rescued its Al tolerance. Expression of FeMATE1 also partially recovered the Al-induced secretion of citrate in the transgenic lines, whereas expression of FeMATE2 did not complement the citrate secretion. Further physiological analysis showed that buckwheat roots also secreted citrate in addition to oxalate in response to Al in a dose-responsive manner. Taken together, our results indicate that FeMATE1 is involved in the Al-activated citrate secretion in the roots, while FeMATE2 is probably responsible for transporting citrate into the Golgi system for the internal detoxification of Al in the roots and leaves of buckwheat.

    DOI: 10.1093/pcp/pcx152

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  • Root cone angle is enlarged in docs1 LRR-RLK mutants in rice

    M. Bettembourg, M. Dal-Soglio, C. Bureau, A. Vernet, A. Dardoux, M. Portefaix, M. Bes, D. Meynard, D. Mieulet, B. Cayrol, C. Perin, B. Courtois, J. F. Ma, A. Dievart

    RICE10 ( 1 ) 453 - 459   2017年12月

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    記述言語:英語   出版者・発行元:SPRINGER  

    The DEFECTIVE IN OUTER CELL LAYER SPECIFICATION 1 (DOCS1) gene belongs to the Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) subfamily. It has been discovered few years ago in Oryza sativa (rice) in a screen to isolate mutants with defects in sensitivity to aluminum. The c68 (docs1-1) mutant possessed a nonsense mutation in the C-terminal part of the DOCS1 kinase domain.
    We have generated a new loss-of-function mutation in the DOCS1 gene (docs1-2) using the CRISPR-Cas9 technology. This new loss-of-function mutant and docs1-1 present similar phenotypes suggesting the original docs1-1 was a null allele. Besides the aluminum sensitivity phenotype, both docs1 mutants shared also several root phenotypes described previously: less root hairs and mixed identities of the outer cell layers. Moreover, our new results suggest that DOCS1 could also play a role in root cap development. We hypothesized these docs1 root phenotypes may affect gravity responses. As expected, in seedlings, the early gravitropic response was delayed. Furthermore, at adult stage, the root gravitropic set angle of docs1 mutants was also affected since docs1 mutant plants displayed larger root cone angles.
    All these observations add new insights into the DOCS1 gene function in gravitropic responses at several stages of plant development.

    DOI: 10.1186/s12284-017-0190-1

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  • A GDSL-motif esterase/acyltransferase/lipase is responsible for leaf water retention in barley

    Chao Li, Guoxiong Chen, Kohei Mishina, Naoki Yamaji, Jian Feng Ma, Fumiko Yukuhiro, Akemi Tagiri, Cheng Liu, Mohammad Pourkheirandish, Nadia Anwar, Masaru Ohta, Pengshan Zhao, Udda Lundqvist, Xinrong Li, Takao Komatsuda

    PLANT DIRECT1 ( 5 ) 1 - 12   2017年11月

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    記述言語:英語   出版者・発行元:JOHN WILEY & SONS LTD  

    The hydrophobic cuticle covers the surface of the most aerial organs of land plants. The barley mutant eceriferum-zv (cer-zv), which is hypersensitive to drought, is unable to accumulate a sufficient quantity of cutin in its leaf cuticle. The mutated locus has been mapped to a 0.02 cM segment in the pericentromeric region of chromosome 4H. As a map-based cloning approach to isolate the gene was therefore considered unlikely to be feasible, a comparison was instead made between the transcriptomes of the mutant and the wild type. In conjunction with extant genomic information, on the basis of predicted functionality, only two genes were considered likely to encode a product associated with cutin formation. When eight independent cer-zv mutant alleles were resequenced with respect to the two candidate genes, it was confirmed that the gene underlying the mutation in each allele encodes a Gly-Asp-Ser-Leu (GDSL)-motif esterase/acyltransferase/lipase. The gene was transcribed in the epidermis, and its product was exclusively deposited in cell wall at the boundary of the cuticle in the leaf elongation zone, coinciding with the major site of cutin deposition. CER-ZV is speculated to function in the deposition of cutin polymer. Its homologs were found in green algae, moss, and euphyllophytes, indicating that it is highly conserved in plant kingdom.

    DOI: 10.1002/pld3.25

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  • Node-controlled allocation of mineral elements in Poaceae

    Naoki Yamaji, Jian Feng Ma

    CURRENT OPINION IN PLANT BIOLOGY39   18 - 24   2017年10月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:CURRENT BIOLOGY LTD  

    Mineral elements taken up by the roots will be delivered to different organs and tissues depending on their requirements. In Poaceae, this selective distribution is mainly mediated in the nodes, which have highly developed and fully organized vascular systems. Inter-vascular transfer of mineral elements from enlarged vascular bundles to diffuse vascular bundles is required for their preferential distribution to developing tissues and reproductive organs. A number of transporters involved in this inter-vascular transfer processes have been identified mainly in rice. They are localized at the different cell layers and form an efficient machinery within the node. Furthermore, some these transporters show rapid response to the environmental changes of mineral elements at the protein level. In addition to the node-based transporters, distinct nodal structures including enlarged xylem area, folded plasma membrane of xylem transfer cells and presence of an apoplastic barrier are also required for the efficient inter-vascular transfer. Manipulation of node-based transporters will provide a novel breeding target to improve nutrient use efficiency, productivity, nutritional value and safety in cereal crops.

    DOI: 10.1016/j.pbi.2017.05.002

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  • Detection of quantitative trait loci controlling grain zinc concentration using Australian wild rice, Oryza meridionalis, a potential genetic resource for biofortification of rice

    Ryo Ishikawa, Masahide Iwata, Kenta Taniko, Gotaro Monden, Naoya Miyazaki, Chhourn Orn, Yuki Tsujimura, Shusaku Yoshida, Jian Feng Ma, Takashige Ishii

    PLOS ONE12 ( 10 ) e0187224   2017年10月

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    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Zinc (Zn) is one of the essential mineral elements for both plants and humans. Zn deficiency in human is one of the major causes of hidden hunger, a serious health problem observed in many developing countries. Therefore, increasing Zn concentration in edible part is an important issue for improving human Zn nutrition. Here, we found that an Australian wild rice O. meridionalis showed higher grain Zn concentrations compared with cultivated and other wild rice species. The quantitative trait loci (QTL) analysis was then performed to identify the genomic regions controlling grain Zn levels using backcross recombinant inbred lines derived from O. sativa 'Nipponbare' and O. meridionalis W1627. Four QTLs responsible for high grain Zn were detected on chromosomes 2, 9, and 10. The QTL on the chromosome 9 (named qGZn9), which showed the largest effect on grain Zn concentration was confirmed with the introgression line, which had a W1627 chromosomal segment covering the qGZn9 region in the genetic background of O. sativa 'Nipponbare'. Fine mapping of this QTL resulted in identification of two tightly linked loci, qGZn9a and qGZn9b. The candidate regions of qGZn9a and qGZn9b were estimated to be 190 and 950 kb, respectively. Furthermore, we also found that plants having a wild chromosomal segment covering qGZn9a, but not qGZn9b, is associated with fertility reduction. qGZn9b, therefore, provides a valuable allele for breeding rice with high Zn in the grains.

    DOI: 10.1371/journal.pone.0187224

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  • Editorial: Role of Silicon in Plants

    Rupesh K. Deshmukh, Jian F. Ma, Richard R. Belanger

    FRONTIERS IN PLANT SCIENCE8   1858   2017年10月

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    記述言語:英語   出版者・発行元:FRONTIERS MEDIA SA  

    DOI: 10.3389/fpls.2017.01858

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  • The Tonoplast-Localized Transporter MTP8.2 Contributes to Manganese Detoxification in the Shoots and Roots of Oryza sativa L.

    Yuma Takemoto, Yuta Tsunemitsu, Miho Fujii-Kashino, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma, Shin-ichiro Kato, Kozo Iwasaki, Daisei Ueno

    PLANT AND CELL PHYSIOLOGY58 ( 9 ) 1573 - 1582   2017年9月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Manganese (Mn) cation diffusion facilitators (Mn-CDFs) play important roles in the Mn homeostasis of plants. In rice, the tonoplast-localized Mn-CDF metal tolerance protein 8.1 (MTP8.1) is involved in Mn detoxification in the shoots. This study functionally characterized the Mn-CDF MTP8.2 and determined its contribution to Mn tolerance. MTP8.2 was found to share 68% identity with MTP8.1 and was expressed in both the shoots and roots, but its transcription level was lower than that of MTP8.1. Transient expression of the MTP8.2:green fluorescent protein (GFP) fusion protein and immunoblotting studies indicated that MTP8.2 was also localized to the tonoplast. MTP8.2 expression in yeast conferred tolerance to Mn but not to Fe, Zn, Co, Ni or Cd. MTP8.2 knockdown caused further growth reduction of shoots and roots in the mtp8.1 mutant, which already exhibits stunted growth under conditions of excess Mn. In the presence of high Mn, the MTP8.2 knockdown lines of the mtp8.1 mutant showed lower root Mn concentrations, as well as lower root: total Mn ratios, than those of wild-type rice and the mtp8.1 mutant. These findings indicate that MTP8.2 mediates Mn tolerance along with MTP8.1 through the sequestration of Mn into the shoot and root vacuoles.

    DOI: 10.1093/pcp/pcx082

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  • Silicon reduces cadmium accumulation by suppressing expression of transporter genes involved in cadmium uptake and translocation in rice

    Ji Feng Shao, Jing Che, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY68 ( 20 ) 5641 - 5651   2017年9月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Silicon (Si) alleviates cadmium (Cd) toxicity and accumulation in a number of plant species, but the exact molecular mechanisms responsible for this effect are still poorly understood. Here, we investigated the effect of Si on Cd toxicity and accumulation in rice (Oryza sativa) by using two mutants (lsi1 and lsi2) defective in Si uptake and their wild types (WTs). Root elongation was decreased with increasing external Cd concentrations in both WTs and mutants, but Si did not show an alleviative effect on Cd toxicity in all lines. By contrast, the Cd concentration in both the shoots and roots was decreased by Si in the WTs, but not in the mutants. Furthermore, Si supply resulted in a decreased Cd concentration in the root cell sap and xylem sap in the WTs, but not in the mutants. Pre-treatment with Si also decreased Cd accumulation in the WTs, but not in the mutants. Silicon slightly decreased Cd accumulation in the cell wall of the roots. The expression level of OsNramp5 and OsHMA2 was down-regulated by Si in the WTs, but not in the mutants. These results indicate that the Si-decreased Cd accumulation was caused by down-regulating transporter genes involved in Cd uptake and translocation in rice.

    DOI: 10.1093/jxb/erx364

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  • Functional characterization of two half-size ABC transporter genes in aluminium-accumulating buckwheat

    Gui Jie Lei, Kengo Yokosho, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

    NEW PHYTOLOGIST215 ( 3 ) 1080 - 1089   2017年8月

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    記述言語:英語   出版者・発行元:WILEY  

    Buckwheat (Fagopyrum esculentum Moench) is able to detoxify high aluminium (Al) internally by sequestering it to the vacuoles in the leaves; however, the molecular mechanisms underlying this sequestration are unknown.We performed proteomic analysis with the leaf tonoplast-rich fraction and identified two half-size ABC transporters; FeASL1.1 and FeALS1.2. We investigated the gene expression patterns and subcellular localization. To demonstrate their physiological role, we expressed FeALS1.1 or FeALS1.2 in the Arabidopsis atals1 mutant under the control of AtALS1 promoter.FeALS1.1 expression was upregulated by Al in both the leaves and the roots, and its expression level in the roots was six times higher than its homologous gene (AtALS1) of Arabidopsis. FeALS1.2 expression, however, was not affected by Al but showed a 39 times higher expression level than AtALS1 in the leaves. When FeALS1.1 or FeALS1.2 was expressed in atals1, both of them recovered their Al tolerance through altering the subcellular localization of Al in root cells.Taken together, our results indicate that FeALS1.1 and FeALS1.2 are involved in the internal detoxification of Al in the roots and leaves, respectively, by sequestering Al into the vacuoles. Their high expression is probably required for high Al tolerance in buckwheat.

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  • OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice

    Natsuko I. Kobayashi, Naoki Yamaji, Hiroki Yamamoto, Kaoru Okubo, Hiroki Ueno, Alex Costa, Keitaro Tanoi, Hideo Matsumura, Miho Fujii-Kashino, Tomoki Horiuchi, Mohammad Al Nayef, Sergey Shabala, Gynheung An, Jian Feng Ma, Tomoaki Horie

    PLANT JOURNAL91 ( 4 ) 657 - 670   2017年8月

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    記述言語:英語   出版者・発行元:WILEY  

    Salt tolerance quantitative trait loci analysis of rice has revealed that the SKC1 locus, which is involved in a higher K+/Na+ ratio in shoots, corresponds to the OsHKT1;5 gene encoding a Na+-selective transporter. However, physiological roles of OsHKT1;5 in rice exposed to salt stress remain elusive, and no OsHKT1;5 gene disruption mutants have been characterized to date. In this study, we dissected two independent TDNA insertional OsHKT1;5 mutants. Measurements of ion contents in tissues and 22 Na+ tracer imaging experiments showed that loss-of-function of OsHKT1;5 in salt-stressed rice roots triggers massive Na+ accumulation in shoots. Salt stress-induced increases in the OsHKT1;5 transcript were observed in roots and basal stems, including basal nodes. Immuno-staining using an anti-OsHKT1;5 peptide antibody indicated that OsHKT1;5 is localized in cells adjacent to the xylem in roots. Additionally, direct introduction of 22 Na+ tracer to leaf sheaths also demonstrated the involvement of OsHKT1;5 in xylem Na+ unloading in leaf sheaths. Furthermore, OsHKT1;5 was indicated to be present in the plasma membrane and found to localize also in the phloem of diffuse vascular bundles in basal nodes. Together with the characteristic 22 Na+ allocation in the blade of the developing immature leaf in the mutants, these results suggest a novel function of OsHKT1;5 in mediating Na+ exclusion in the phloem to prevent Na+ transfer to young leaf blades. Our findings further demonstrate that the function of OsHKT1;5 is crucial over growth stages of rice, including the protection of the next generation seeds as well as of vital leaf blades under salt stress.

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  • A Model of Silicon Dynamics in Rice: An Analysis of the Investment Efficiency of Si Transporters

    Gen Sakurai, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Keisuke Ono, Jian Feng Ma

    FRONTIERS IN PLANT SCIENCE8   1187   2017年7月

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    記述言語:英語   出版者・発行元:FRONTIERS MEDIA SA  

    Silicon is the second most abundant element in soils and is beneficial for plant growth. Although, the localizations and polarities of rice Si transporters have been elucidated, the mechanisms that control the expression of Si transporter genes and the functional reasons for controlling expression are not well-understood. We developed a new model that simulates the dynamics of Si in the whole plant in rice by considering Si transport in the roots, distribution at the nodes, and signaling substances controlling transporter gene expression. To investigate the functional reason for the diurnal variation of the expression level, we compared investment efficiencies (the amount of Si accumulated in the upper leaf divided by the total expression level of Si transporter genes) at different model settings. The model reproduced the gradual decrease and diurnal variation of the expression level of the transporter genes observed by previous experimental studies. The results of simulation experiments showed that a considerable reduction in the expression of Si transporter genes during the night increases investment efficiency. Our study suggests that rice has a system that maximizes the investment efficiency of Si uptake.

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  • A Magnesium Transporter OsMGT1 Plays a Critical Role in Salt Tolerance in Rice(1[OPEN])

    Zhi Chang Chen, Naoki Yamaji, Tomoaki Horie, Jing Che, Jian Li, Gynheung An, Jian Feng Ma

    PLANT PHYSIOLOGY174 ( 3 ) 1837 - 1849   2017年7月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Salt stress is one of the major factors limiting rice (Oryza sativa) production globally. Although several transporters involved in salt tolerance have been identified in rice, the mechanisms regulating their transport activity are still poorly understood. Here, we show evidence that a rice Mg transporter OsMGT1 is required for salt tolerance probably by regulating transport activity of OsHKT1;5, a key transporter for the removal of Na+ from the xylem sap at the root mature zone. Knockout of OsMGT1 did not affect total Na uptake, but increased Na concentration in the shoots and xylem sap, resulting in a significant increase in salt sensitivity at low external Mg2+ concentration (20-200 mu M). However, such differences were abolished at a higher Mg2+ concentration (2 mM), although the total Na uptake was not altered. OsMGT1 was expressed in both the roots and shoots, but only that in the roots was moderately up-regulated by salt stress. Spatial expression analysis revealed that OsMGT1 was expressed in all root cells of the root tips but was highly expressed in the pericycle of root mature zone. OsMGT1 was also expressed in the phloem region of basal node, leaf blade, and sheath. When expressed in Xenopus laevis oocytes, the transport activity of OsHKT1;5 was enhanced by elevating external Mg2+ concentration. Furthermore, knockout of OsHKT1;5 in osmgt1 mutant background did not further increase its salt sensitivity. Taken together, our results suggest that Mg2+ transported by OsMGT1 in the root mature zone is required for enhancing OsHKT1;5 activity, thereby restricting Na accumulation to the shoots.

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  • Identification of a mammalian silicon transporter

    Sarah Ratcliffe, Ravin Jugdaohsingh, Julien Vivancos, Alan Marron, Rupesh Deshmukh, Jian Feng Ma, Namiki Mitani-Ueno, Jack Robertson, John Wills, Mark V. Boekschoten, Michael Mueller, Robert C. Mawhinney, Stephen D. Kinrade, Paul Isenring, Richard R. Belanger, Jonathan J. Powell

    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY312 ( 5 ) C550 - C561   2017年5月

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    記述言語:英語   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Silicon (Si) has long been known to play a major physiological and structural role in certain organisms, including diatoms, sponges, and many higher plants, leading to the recent identification of multiple proteins responsible for Si transport in a range of algal and plant species. In mammals, despite several convincing studies suggesting that silicon is an important factor in bone development and connective tissue health, there is a critical lack of understanding about the biochemical pathways that enable Si homeostasis. Here we report the identification of a mammalian efflux Si transporter, namely Slc34a2 (also termed NaPiIIb), a known sodium-phosphate cotransporter, which was upregulated in rat kidney following chronic dietary Si deprivation. Normal rat renal epithelium demonstrated punctate expression of Slc34a2, and when the protein was heterologously expressed in Xenopus laevis oocytes, Si efflux activity (i.e., movement of Si out of cells) was induced and was quantitatively similar to that induced by the known plant Si transporter OsLsi2 in the same expression system. Interestingly, Si efflux appeared saturable over time, but it did not vary as a function of extracellular HPO42- or Na+ concentration, suggesting that Slc34a2 harbors a functionally independent transport site for Si operating in the reverse direction to the site for phosphate. Indeed, in rats with dietary Si depletion-induced upregulation of transporter expression, there was increased urinary phosphate excretion. This is the first evidence of an active Si transport protein in mammals and points towards an important role for Si in vertebrates and explains interactions between dietary phosphate and silicon.

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  • Polar Localization of the NIP5;1 Boric Acid Channel Is Maintained by Endocytosis and Facilitates Boron Transport in Arabidopsis Roots

    Sheliang Wang, Akira Yoshinari, Tomoo Shimada, Ikuko Hara-Nishimura, Namiki Mitani-Ueno, Jian Feng Ma, Satoshi Naito, Junpei Takano

    PLANT CELL29 ( 4 ) 824 - 842   2017年4月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Boron uptake in Arabidopsis thaliana is mediated by nodulin 26-like intrinsic protein 5;1 (NIP5;1), a boric acid channel that is located preferentially on the soil side of the plasma membrane in root cells. However, the mechanism underlying this polar localization is poorly understood. Here, we show that the polar localization of NIP5;1 in epidermal and endodermal root cells is mediated by the phosphorylation of Thr residues in the conserved TPG (ThrProGly) repeat in the N-terminal region of NIP5;1. Although substitutions of Ala for three Thr residues in the TPG repeat did not affect lateral diffusion in the plasma membrane, these substitutions inhibited endocytosis and strongly compromised the polar localization of GFP-NIP5;1. Consistent with this, the polar localization was compromised in m subunit mutants of the clathrin adaptor AP2. The Thr-to-Ala substitutions did not affect the boron transport activity of GFP-NIP5;1 in Xenopus laevis oocytes but did inhibit the ability to complement boron translocation to shoots and rescue growth defects in nip5;1-1 mutant plants under boron-limited conditions. These results demonstrate that the polar localization of NIP5;1 is maintained by clathrin-mediated endocytosis, is dependent on phosphorylation in the TPG repeat, and is necessary for the efficient transport of boron in roots.

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  • The Key to Vn Homeostasis in Plants: Regulation of Mn Transporters

    Ji Feng Shao, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    TRENDS IN PLANT SCIENCE22 ( 3 ) 215 - 224   2017年3月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:ELSEVIER SCIENCE LONDON  

    Plants only require small amounts of manganese (Mn) for healthy growth, but Mn concentrations in soil solution vary from sub-micromolar to hundreds of micromolar across the growth period. Therefore, plants must deal with large Mn concentration fluctuations, but the molecular mechanisms underlying how plants cope with low and high Mn concentrations are poorly understood. In this Opinion we discuss the role of Mn transporters in the uptake, distribution, and detoxification of Mn in response to changes in Mn concentrations through their regulation at the transcriptional and protein levels, mainly focusing on rice, an Mn-tolerant and accumulating species. We also propose mechanisms involved in the hyperaccumulation of Mn and future prospects for studying this specific trait.

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  • Isolation and characterization of a rice line with high Cd accumulation for potential use in phytoremediation

    Ji Feng Shao, Miho Fujii-Kashino, Naoki Yamaji, Shinichi Fukuoka, Ren Fang Shen, Jian Feng Ma

    PLANT AND SOIL410 ( 1-2 ) 357 - 368   2017年1月

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    記述言語:英語   出版者・発行元:SPRINGER  

    To reduce cadmium (Cd) intake, remediation of Cd-contaminated soil and breeding crops with low Cd accumulation are important. This study aims to isolate rice mutants with altered accumulation of Cd.We used rice seeds mutated by N-methyl-N-nitrosourea for screening. The mutant was physiologically, genetically, and molecularly characterized. Cd accumulation was compared among five rice varieties cultivated in a Cd-contaminated soil.From 1000 lines screened, we isolated a line (TCM213) with high Cd accumulation. There was no difference in the root Cd uptake, but a higher root-to-shoot translocation of Cd was found in TCM213 compared with a common rice cultivar, T-65. The expression and sequence of OsNramp5 and OsHMA2 did not differ between TCM213 and T-65. However, several SNPs and deletion were found in the sequence of OsHMA3, although its expression and tissue localization were similar to those of T-65. Genetic analysis of an F-2 population derived from T-65 and TCM213 showed that the variation of OsHMA3 explained 72 % of variation in total Cd accumulation. TCM213 accumulated the largest Cd amount in the shoots among five Cd-accumulating varieties.High Cd accumulation in TCM213 results from loss of function of OsHMA3, and its high Cd accumulation has potential for efficient phytoremediation of Cd-contaminated soil.

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  • Iron-induced nitric oxide leads to an increase in the expression of ferritin during the senescence of Lotus japonicus nodules

    Sirinapa Chungopast, Mallika Duangkhet, Shigeyuki Tajima, Jian Feng Ma, Mika Nomura

    JOURNAL OF PLANT PHYSIOLOGY208   40 - 46   2017年1月

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    記述言語:英語   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Iron is an essential nutrient for legume-rhizobium symbiosis and accumulates abundantly in the nodules. However, the concentration of free iron in the cells is strictly controlled to avoid toxicity. It is known that ferritin accumulates in the cells as an iron storage protein. During nodule senescence, the expression of the ferritin gene, Ljfer1, was induced in Lotus japonicus. We investigated a signal transduction pathway leading to the increase of Ljfer1 in the nodule. The Ljfer1 promoter of L. japonicus contains a conserved Iron-Dependent Regulatory Sequence (IDRS). The expression of Ljfer1 was induced by the application of iron or sodium nitroprusside, which is a nitric oxide (NO) donor. The application of iron to the nodule increased the level of NO. These data strongly suggest that iron-induced NO leads to increased expression of Ljfer1 during the senescence of L. japonicus nodules. (C) 2016 Elsevier GmbH. All rights reserved.

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  • Reducing phosphorus accumulation in rice grains with an impaired transporter in the node

    Naoki Yamaji, Yuma Takemoto, Takaaki Miyaji, Namiki Mitani-Ueno, Kaoru T. Y. Oshida, Jian Feng Ma

    NATURE541 ( 7635 ) 92 - +   2017年1月

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    記述言語:英語   出版者・発行元:NATURE RESEARCH  

    Phosphorus is an important nutrient for crop productivity. More than 60% of the total phosphorus in cereal crops is finally allocated into the grains and is therefore removed at harvest. This removal accounts for 85% of the phosphorus fertilizers applied to the field each year(1,2). However, because humans and non-ruminants such as poultry, swine and fish cannot digest phytate, the major form of phosphorus in the grains, the excreted phosphorus causes eutrophication of waterways. A reduction in phosphorus accumulation in the grain would contribute to sustainable and environmentally friendly agriculture. Here we describe a rice transporter, SULTR-like phosphorus distribution transporter (SPDT), that controls the allocation of phosphorus to the grain. SPDT is expressed in the xylem region of both enlarged-and diffuse-vascular bundles of the nodes, and encodes a plasma-membrane-localized transporter for phosphorus. Knockout of this gene in rice (Oryza sativa) altered the distribution of phosphorus, with decreased phosphorus in the grains but increased levels in the leaves. Total phosphorus and phytate in the brown de-husked rice were 20-30% lower in the knockout lines, whereas yield, seed germination and seedling vigour were not affected. These results indicate that SPDT functions in the rice node as a switch to allocate phosphorus preferentially to the grains. This finding provides a potential strategy to reduce the removal of phosphorus from the field and lower the risk of eutrophication of waterways.

    DOI: 10.1038/nature20610

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  • 4-2-10 ソバのNrampファミリー遺伝子の機能解析(4-2 植物の微量栄養素,2017年度仙台大会)

    横正 健剛, 邵 継鋒, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集63 ( 0 ) 66 - 66   2017年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice

    Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY57 ( 12 ) 2510 - 2518   2016年12月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Rice requires high silicon (Si) for its high and sustainable yield. The efficient uptake of Si in rice is mediated by two transporters OsLsi1 and OsLsi2, which function as influx and efflux transporters, respectively. Our previous studies showed that the mRNA expression levels of these transporter genes were down-regulated by Si. Herein we investigated the mechanism underlying regulation of OsLsi1 and OsLsi2 expression. There was a negative correlation between the expression level of OsLsi1 and OsLsi2 and shoot Si accumulation when the rice seedlings were exposed to different Si supply conditions. A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also downregulated in half the roots without direct Si exposure when the other half of the roots were exposed to Si. Analysis with transgenic rice carrying different lengths of OsLsi1 promoter regions fused with green fluorescent protein (GFP) as a reporter gene revealed that the region responsible for the Si response of OsLsi1 expression is present between -327 to -292 in the promoter. However, this region was not associated with the tissue and cellular localization of OsLsi1. In conclusion, the Si-induced down-regulation of Si transporter genes is controlled by shoot Si, not root Si, and the region between -327 and -292 in the OsLsi1 promoter is involved in this regulation of OsLsi1 expression in rice.

    DOI: 10.1093/pcp/pcw163

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  • Retrotransposon-Mediated Aluminum Tolerance through Enhanced Expression of the Citrate Transporter OsFRDL4

    Kengo Yokosho, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

    PLANT PHYSIOLOGY172 ( 4 ) 2327 - 2336   2016年12月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    High aluminum (Al) tolerance of rice (Oryza sativa) is controlled by multiple tolerance genes, but the regulatory mechanisms underlying the differential expression of these genes are poorly understood. Here, we investigated the factors regulating the expression of OsFRDL4, a gene encoding a citrate efflux transporter involved in Al-induced citrate secretion from the roots. Analysis with chromosome segment substitution lines derived from cv Nipponbare (high OsFRDL4 expression) and cv Kasalath (low OsFRDL4 expression) revealed that the differential expression of OsFRDL4 is responsible for the quantitative trait locus for Al tolerance detected previously on chromosome 1. Comparison of the OsFRDL4 gene structure in cv Nipponbare and cv Kasalath showed that there was no difference in the position of the transcriptional start site, but a 1.2-kb insertion showing high similarity to the solo long terminal repeat of the retrotransposon was found in the promoter region of OsFRDL4 in cv Nipponbare. This insertion showed higher promoter activity and contained nine cis-acting elements for ALUMINUM RESISTANCE TRANSCRIPTION FACTOR1 (ART1). However, this insertion did not alter the spatial expression or cellular localization of OsFRDL4. Furthermore, this insertion was found in most japonica varieties but was largely absent from indica varieties or wild rice species. These results indicate that the 1.2-kb insertion in the OsFRDL4 promoter region in japonica subspecies is responsible for their higher expression level of OsFRDL4 due to the increased number of cis-acting elements of ART1. Our results also suggest that this insertion event happened at the initial stage of domestication of japonica subspecies.

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  • The HvNramp5 Transporter Mediates Uptake of Cadmium and Manganese, But Not Iron 国際誌

    Dezhi Wu, Naoki Yamaji, Miki Yamane, Miho Kashino-Fujii, Kazuhiro Sato, Jian Feng Ma

    PLANT PHYSIOLOGY172 ( 3 ) 1899 - 1910   2016年11月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    The Natural Resistance Associated Macrophage Protein (Nramp) represents a transporter family for metal ions in all organisms. Here, we functionally characterized a member of Nramp family in barley (Hordeum vulgare), HvNramp5. This member showed different expression patterns, transport substrate specificity, and cellular localization from its close homolog in rice (Oryza sativa), OsNramp5, although HvNramp5 was also localized to the plasma membrane. HvNramp5 was mainly expressed in the roots and its expression was not affected by Cd and deficiency of Zn, Cu, and Mn, but slightly up-regulated by Fe deficiency. Spatial expression analysis showed that the expression of HvNramp5 was higher in the root tips than that in the basal root regions. Furthermore, analysis with laser microdissection revealed higher expression of HvNramp5 in the outer root cell layers. HvNramp5 showed transport activity for both Mn2+ and Cd2+, but not for Fe2+ when expressed in yeast. Immunostaining with a HvNramp5 antibody showed that this protein was localized in the root epidermal cells without polarity. Knockdown of HvNramp5 in barley resulted in a significant reduction in the seedling growth at low Mn supply, but this reduction was rescued at high Mn supply. The concentration of Mn and Cd, but not other metals including Cu, Zn, and Fe, was decreased in both the roots and shoots of knockdown lines compared with the wild-type barley. These results indicate that HvNramp5 is a transporter required for uptake of Mn and Cd, but not for Fe, and that barley has a distinct uptake system from rice.

    DOI: 10.1104/pp.16.01189

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  • An Al-inducible expansin gene, OsEXPA10 is involved in root cell elongation of rice

    Jing Che, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    PLANT JOURNAL88 ( 1 ) 132 - 142   2016年10月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Expansins are cell wall loosening proteins, which are encoded by multigene families. However, the physiological role of most expansin genes is still poorly understood. Here, we functionally characterized an Al-inducible expansin gene, OsEXPA10, which is regulated by a C2H2-type zinc-finger transcription factor, ART1 in rice. A detailed expression analysis showed that OsEXPA10 was expressed in both the roots and shoots at a similar level, but only the expression in the roots was rapidly upregulated in response to Al. Furthermore, spatial expression analysis showed that the Al-induced expression was only found in the root tips (0-3 mm), but not in the mature root zones. The expression was neither induced by other metals including Cd and La nor by low pH. Immunostaining showed that OsEXPA10 was localized at all cells of the root tips. Knockout of OsEXPA10 resulted in a significant decrease in the cell elongation of the roots in the absence of Al. In the presence of Al, knockout of OsEXPA10 did not alter the Al sensitivity evaluated by relative root elongation, but the root cell wall of knockout lines accumulated less Al compared to those of the wild-type rice. Collectively, our results indicate that OsEXPA10 expressed in the root tips is required for the root cell elongation, but that the contribution of this gene to high Al tolerance in rice is small.

    DOI: 10.1111/tpj.13237

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  • An Al-inducible expansin gene, OsEXPA10 is involved in root cell elongation of rice

    Jing Che, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    PLANT JOURNAL88 ( 1 ) 132 - 142   2016年10月

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    記述言語:英語   出版者・発行元:WILEY  

    Expansins are cell wall loosening proteins, which are encoded by multigene families. However, the physiological role of most expansin genes is still poorly understood. Here, we functionally characterized an Al-inducible expansin gene, OsEXPA10, which is regulated by a C2H2-type zinc-finger transcription factor, ART1 in rice. A detailed expression analysis showed that OsEXPA10 was expressed in both the roots and shoots at a similar level, but only the expression in the roots was rapidly upregulated in response to Al. Furthermore, spatial expression analysis showed that the Al-induced expression was only found in the root tips (0-3 mm), but not in the mature root zones. The expression was neither induced by other metals including Cd and La nor by low pH. Immunostaining showed that OsEXPA10 was localized at all cells of the root tips. Knockout of OsEXPA10 resulted in a significant decrease in the cell elongation of the roots in the absence of Al. In the presence of Al, knockout of OsEXPA10 did not alter the Al sensitivity evaluated by relative root elongation, but the root cell wall of knockout lines accumulated less Al compared to those of the wild-type rice. Collectively, our results indicate that OsEXPA10 expressed in the root tips is required for the root cell elongation, but that the contribution of this gene to high Al tolerance in rice is small.

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  • OsFRDL1 expressed in nodes is required for distribution of iron to grains in rice

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY67 ( 18 ) 5485 - 5494   2016年10月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    OsFRDL1 expressed in the upper nodes is required for the distribution of Fe to the panicles through solubilizing Fe deposited in the apoplastic part of nodes in rice.Iron (Fe) is essential for plant growth and development, but the molecular mechanisms underlying its distribution to different organs are poorly understood. We found that OsFRDL1 (FERRIC REDUCTASE DEFECTIVE LIKE 1), a plasma membrane-localized transporter for citrate, was highly expressed in the upper nodes of rice at the reproductive growth stage. OsFRDL1 was expressed in most cells of enlarged vascular bundles, diffuse vascular bundles, and the interjacent parenchyma cell bridges of uppermost node I, as well as vascular tissues of the leaf blade, leaf sheath, peduncle, rachis, husk, and stamen. Knockout of OsFRDL1 decreased pollen viability and grain fertility when grown in a paddy field. Iron was deposited in the parenchyma cell bridges, a few of the cell layers of the parenchyma tissues outside of the bundle sheath of enlarged vascular bundles in node I in both the wild-type rice and osfrdl1 mutant, but the mutant accumulated more Fe than the wild-type rice in this area. A stem-fed experiment with stable isotope Fe-57 showed that the distribution of Fe in the anther and panicle decreased in the knockout line, but that in the flag leaf it increased compared with the wild-type rice. Taken together, our results show that OsFRDL1 expressed in the upper nodes is required for the distribution of Fe in the panicles through solubilizing Fe deposited in the apoplastic part of nodes in rice.

    DOI: 10.1093/jxb/erw314

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  • A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain

    Xin-Yuan Huang, Fenglin Deng, Naoki Yamaji, Shannon R. M. Pinson, Miho Fujii-Kashino, John Danku, Alex Douglas, Mary Lou Guerinot, David E. Salt, Jian Feng Ma

    NATURE COMMUNICATIONS7   2016年7月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Rice is a major source of calories and mineral nutrients for over half the world's human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of a quantitative trait locus controlling Cu accumulation in rice grain. We provide evidence that OsHMA4 functions to sequester Cu into root vacuoles, limiting Cu accumulation in the grain. The difference in grain Cu accumulation is most likely attributed to a single amino acid substitution that leads to different OsHMA4 transport activity. The allele associated with low grain Cu was found in 67 of the 1,367 rice accessions investigated. Identification of natural allelic variation in OsHMA4 may facilitate the development of rice varieties with grain Cu concentrations tuned to both the concentration of Cu in the soil and dietary needs.

    DOI: 10.1038/ncomms12138

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  • An Aluminum-Inducible IREG Gene is Required for Internal Detoxification of Aluminum in Buckwheat

    Kengo Yokosho, Naoki Yamaji, Namiki Mitani-Ueno, Ren Fang Shen, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY57 ( 6 ) 1169 - 1178   2016年6月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Buckwheat (Fagopyrum esculentum Moench) is able to detoxify aluminum (Al) both externally and internally, but the molecular mechanisms underlying its high Al tolerance are not understood. We functionally characterized a gene (FeIREG1) belonging to IRON REGULATED/ferroportin in buckwheat, which showed high expression in our previous genome-wide transcriptome analysis. FeIREG1 was mainly expressed in the roots, and its expression was up-regulated by Al, but not by other metals and low pH. Furthermore, in contrast to AtIREG1 and AtIREG2 in Arabidopsis, the expression of FeIREG1 was not induced by Fe deficiency. Spatial expression analysis showed that the Al-induced expression of FeIREG1 was found in the root tips and higher expression was detected in the outer layers of this part. Immunostaining also showed that FeIREG1 was localized at the outer cell layers in the root tip. A FeIREG1-green fluorescent protein (GFP) fusion protein was localized to the tonoplast when transiently expressed in onion epidermal cells. Overexpression of FeIREG1 in Arabidopsis resulted in increased Al tolerance, but did not alter the tolerance to Cd, Co and Fe. The tolerance to Ni was slightly enhanced in the overexpression lines. Mineral analysis showed that the accumulation of total root Al and other essential mineral elements was hardly altered in the overexpression lines. Taken together, our results suggest that FeIREG1 localized at the tonoplast plays an important role in internal Al detoxification by sequestering Al into the root vacuoles in buckwheat.

    DOI: 10.1093/pcp/pcw065

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  • Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice

    Yosuke Toda, Yin Wang, Akira Takahashi, Yuya Kawai, Yasuomi Tada, Naoki Yamaji, Jian Feng Ma, Motoyuki Ashikari, Toshinori Kinoshita

    PLANT AND CELL PHYSIOLOGY57 ( 6 ) 1220 - 1230   2016年6月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    The stomatal apparatus consists of a pair of guard cells and regulates gas exchange between the leaf and atmosphere. In guard cells, blue light (BL) activates H+-ATPase in the plasma membrane through the phosphorylation of its penultimate threonine, mediating stomatal opening. Although this regulation is thought to be widely adopted among kidney-shaped guard cells in dicots, the molecular basis underlying that of dumbbell-shaped guard cells in monocots remains unclear. Here, we show that H+-ATPases are involved in the regulation of dumbbell-shaped guard cells. Stomatal opening of rice was promoted by the H+-ATPase activator fusicoccin and by BL, and the latter was suppressed by the H+-ATPase inhibitor vanadate. Using H+-ATPase antibodies, we showed the presence of phosphoregulation of the penultimate threonine in Oryza sativa H+-ATPases (OSAs) and localization of OSAs in the plasma membrane of guard cells. Interestingly, we identified one H+-ATPase isoform, OSA7, that is preferentially expressed among the OSA genes in guard cells, and found that loss of function of OSA7 resulted in partial insensitivity to BL. We conclude that H+-ATPase is involved in BL-induced stomatal opening of dumbbell-shaped guard cells in monocotyledon species.

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  • Transporters involved in mineral nutrient uptake in rice

    Akimasa Sasaki, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY67 ( 12 ) 3645 - 3653   2016年6月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    One of the most important roles of plant roots is to take up essential mineral nutrients from the soil for use in plant growth and development. The uptake of mineral elements is mediated by various transporters belonging to different transporter families. Here we reviewed transporters for the uptake of macronutrients and micronutrients identified in rice, an important staple food for half of the world's population. Rice roots are characterized by having two Casparian strips on the exodermis and endodermis and by the formation of aerenchyma in the mature root zone. This distinct anatomical structure dictates that a pair of influx and efflux transporters at both the exodermis and endodermis is required for the radial transport of a mineral element from the soil solution to the stele. Some transporters showing polar localization at the distal and proximal sides of the exodermis and endodermis have been identified for silicon and manganese, forming an efficient uptake system. However, transporters for the uptake of most mineral elements remain to be identified.

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  • Functional Analysis of a MATE Gene OsFRDL2 Revealed its Involvement in Al-Induced Secretion of Citrate, but a Lower Contribution to Al Tolerance in Rice

    Kengo Yokosho, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY57 ( 5 ) 976 - 985   2016年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    The multidrug and toxic compound extrusion (MATE) transporters represent a large transporter family in plants, but the role of most genes in this family has not been examined. We functionally characterized a MATE family member, OsFRDL2, in rice (Oryza sativa). OsFRDL2 showed an efflux transport activity for citrate when it was expressed in both Xenopus oocytes and cultured tobacco cells. OsFRDL2 was mainly expressed in the roots and its expression was not induced by iron (Fe) deficiency, but it was rapidly up-regulated by aluminum (Al). Furthermore, the expression of OsFRDL2 was regulated by ART1, a C2H2-type zinc-finger transcription factor for Al tolerance. OsFRDL2 protein was localized at unidentified vesicles in the cytosol, but not co-localized with either mitochondria or peroxisomes when expressed in both onion epidermal cells and cultured tobacco cells. Knockout of OsFRDL2 decreased Al-induced secretion of citrate from the roots, but did not affect the internal citrate concentration. The Al-induced inhibition of root elongation was similar between the OsFRDL2 knockout line and its wild-type rice. Knockout of OsFRDL2 did not affect the translocation of Fe from the roots to the shoots. A double mutant between osfrdl2 and osfrdl4 or osfrdl1 did not further decrease the Al-induced citrate secretion and Fe translocation compared with the single mutant. Collectively, our results indicate that although OsFRDL2 is involved in the Al-induced secretion of citrate, its contribution to high Al tolerance is relatively small in rice.

    DOI: 10.1093/pcp/pcw026

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  • A Cation-Chloride Cotransporter Gene Is Required for Cell Elongation and Osmoregulation in Rice

    Zhi Chang Chen, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

    PLANT PHYSIOLOGY171 ( 1 ) 494 - 507   2016年5月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) is characterized by having fibrous root systems; however, the molecular mechanisms underlying the root development are not fully understood. Here, we isolated a rice mutant with short roots and found that the mutant had a decreased cell size of the roots and shoots compared with wild-type rice. Map-based cloning combined with whole-genome sequencing revealed that a single nucleotide mutation occurred in a gene, which encodes a putative cation-chloride cotransporter (OsCCC1). Introduction of OsCCC1 cDNA into the mutant rescued the mutant growth, indicating that growth defects of both the roots and shoots are caused by loss of function of OsCCC1. Physiological analysis showed that the mutant had a lower concentration of Cl- and K+ and lower osmolality in the root cell sap than the wild type at all KCl supply conditions tested; however, the mutant only showed a lower Na+ concentration at high external Na+. Expression of OsCCC1 in yeast increased accumulation of K+, Na+, and Cl-. The expression of OsCCC1 was found in both the roots and shoots, although higher expression was found in the root tips. Furthermore, the expression in the roots did not respond to different Na+, K+, and Cl- supply. OsCCC1 was expressed in all cells of the roots, leaf, and basal node. Immunoblot analysis revealed that OsCCC1 was mainly localized to the plasma membrane. These results suggest that OsCCC1 is involved in the cell elongation by regulating ion (Cl-, K+, and Na+) homeostasis to maintain cellular osmotic potential.

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  • Silicon decreases both uptake and root-to-shoot translocation of manganese in rice

    Jing Che, Naoki Yamaji, Ji Feng Shao, Jian Feng Ma, Ren Fang Shen

    JOURNAL OF EXPERIMENTAL BOTANY67 ( 5 ) 1535 - 1544   2016年3月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Silicon (Si) is known to alleviate manganese (Mn) toxicity in a number of plant species; however, the mechanisms responsible for this effect are poorly understood. Here, we investigated the interaction between Si and Mn in rice (Oryza sativa) by using a mutant defective in Si uptake. Silicon alleviated Mn toxicity in the wild-type (WT) rice, but not in the mutant exposed to high Mn. The Mn concentration in the shoots was decreased, but that in the roots was increased by Si in the WT. In contrast, the Mn concentration in the roots and shoots was unaffected by Si in the mutant. Furthermore, Si supply resulted in an increased Mn in the root cell sap, decreased Mn in the xylem sap in the WT, but these effects of Si were not observed in the mutant. A short-term labelling experiment with Mn-54 showed that the uptake of Mn was similar between plants with and without Si and between WT and the mutant. However, Si decreased root-to-shoot translocation of Mn in the WT, but not in the mutant. The expression of a Mn transporter gene for uptake, OsNramp5, was unaffected by a short exposure (< 1 d) to Si, but down-regulated by relatively long-term exposure to Si in WT. In contrast, the expression of OsNramp5 was unaffected by Si in the mutant. These results indicated that Si-decreased Mn accumulation results from both Si-decreased root-to-shoot translocation of Mn, probably by the formation of Mn-Si complex in root cells, and uptake by down-regulating Mn transporter gene.

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  • OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress

    Kei Suzuki, Naoki Yamaji, Alex Costa, Eiji Okuma, Natsuko I. Kobayashi, Tatsuhiko Kashiwagi, Maki Katsuhara, Cun Wang, Keitaro Tanoi, Yoshiyuki Murata, Julian I. Schroeder, Jian Feng Ma, Tomoaki Horie

    BMC PLANT BIOLOGY16 ( 1 )   2016年1月

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    記述言語:英語   出版者・発行元:BMC  

    Background: Na+ exclusion from leaf blades is one of the key mechanisms for glycophytes to cope with salinity stress. Certain class I transporters of the high-affinity K+ transporter (HKT) family have been demonstrated to mediate leaf blade-Na+ exclusion upon salinity stress via Na+-selective transport. Multiple HKT1 transporters are known to function in rice (Oryza sativa). However, the ion transport function of OsHKT1;4 and its contribution to the Na+ exclusion mechanism in rice remain to be elucidated.Results: Here, we report results of the functional characterization of the OsHKT1; 4 transporter in rice. OsHKT1; 4 mediated robust Na+ transport in Saccharomyces cerevisiae and Xenopus laevis oocytes. Electrophysiological experiments demonstrated that OsHKT1; 4 shows strong Na+ selectivity among cations tested, including Li+, Na+, K+, Rb+, Cs+, and NH4+, in oocytes. A chimeric protein, EGFP-OsHKT1;4, was found to be functional in oocytes and targeted to the plasma membrane of rice protoplasts. The level of OsHKT1; 4 transcripts was prominent in leaf sheaths throughout the growth stages. Unexpectedly however, we demonstrate here accumulation of OsHKT1;4 transcripts in the stem including internode II and peduncle in the reproductive growth stage. Moreover, phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress. However, imposition of salinity stress on the RNAi plants in the reproductive growth stage caused significant Na+ overaccumulation in aerial organs, in particular, leaf blades and sheaths. In addition, Na-22(+) tracer experiments using peduncles of RNAi and WT plants suggested xylem Na+ unloading by OsHKT1;4.Conclusions: Taken together, our results indicate a newly recognized function of OsHKT1;4 in Na+ exclusion in stems together with leaf sheaths, thus excluding Na+ from leaf blades of a japonica rice cultivar in the reproductive growth stage, but the contribution is low when the plants are in the vegetative growth stage.

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  • Toxic Heavy Metal and Metalloid Accumulation in Crop Plants and Foods

    Stephan Clemens, Jian Feng Ma

    ANNUAL REVIEW OF PLANT BIOLOGY, VOL 6767   489 - 512   2016年

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    記述言語:英語   出版者・発行元:ANNUAL REVIEWS  

    Arsenic, cadmium, lead, and mercury are toxic elements that are almost ubiquitously present at low levels in the environment because of anthropogenic influences. Dietary intake of plant-derived food represents a major fraction of potentially health-threatening human exposure, especially to arsenic and cadmium. In the interest of better food safety, it is important to reduce toxic element accumulation in crops. A molecular understanding of the pathways responsible for this accumulation can enable the development of crop varieties with strongly reduced concentrations of toxic elements in their edible parts. Such understanding is rapidly progressing for arsenic and cadmium but is in its infancy for lead and mercury. Basic discoveries have been made in Arabidopsis, rice, and other models, and most advances in crops have been made in rice. Proteins mediating the uptake of arsenic and cadmium have been identified, and the speciation and biotransformations of arsenic are now understood. Factors controlling the efficiency of root-to-shoot translocation and the partitioning of toxic elements through the rice node have also been identified.

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  • 4-3-9 Further characterization of two Al-inducible MATE genes in buckwheat(4-3 植物の有害元素,2016年度佐賀大会)

    雷 貴傑, 横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集62 ( 0 ) 65 - 65   2016年

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    記述言語:英語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 4-3-18 イネ科作物の土壌pH適応におけるトレードオフの可能性(4-3 植物の有害元素,2016年度佐賀大会)

    山地 直樹, 柏野 美帆, 横正 健剛, 馬 建鋒

    日本土壌肥料学会講演要旨集62 ( 0 ) 68 - 68   2016年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 4-2-4 シロイヌナズナホウ酸チャネルNIP5;1の細胞膜上偏在性は効率的なホウ酸輸送に貢献する(4-2 植物の微量栄養素,2016年度佐賀大会)

    Wang Shelling, 永森 彩奈, 三谷 奈見季, 馬 建鋒, 内藤 哲, 高野 順平

    日本土壌肥料学会講演要旨集62 ( 0 ) 58 - 58   2016年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P4-1-6 イネ由来ケイ酸輸送体Lsi1の更なる解析(ポスター,4-1 植物の多量栄養素,2016年度佐賀大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集62 ( 0 ) 52 - 52   2016年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 16 イネのケイ酸吸収に関与する輸送体 Lsi1 と Lsi2 の更なる解析(関西支部講演会,2015年度各支部会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集62 ( 0 ) 284 - 284   2016年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • A polarly localized transporter for efficient manganese uptake in rice

    Daisei Ueno, Akimasa Sasaki, Naoki Yamaji, Takaaki Miyaji, Yumi Fujii, Yuma Takemoto, Sawako Moriyama, Jing Che, Yoshinori Moriyama, Kozo Iwasaki, Jian Feng Ma

    NATURE PLANTS1 ( 12 )   2015年11月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.

    DOI: 10.1038/NPLANTS.2015.170

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  • Genome-wide association mapping of cadmium accumulation in different organs of barley 国際誌

    Dezhi Wu, Kazuhiro Sato, Jian Feng Ma

    NEW PHYTOLOGIST208 ( 3 ) 817 - 829   2015年11月

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    記述言語:英語   出版者・発行元:WILEY  

    The threshold value of cadmium (Cd) concentration in grains of barley (Hordeum vulgare) is the lowest among cereal crops; however, it is poorly understood how Cd accumulation in barley grain is genetically controlled.We investigated genotypic variation in Cd accumulation of different organs in 100 accessions from a subset of the barley core collection using both hydroponic and Cd-contaminated soil culture. We also performed a genome-wide association (GWA) mapping for Cd accumulation in different organs.A large genotypic variation in the Cd concentration was found in all organs. There was a good correlation between shoot Cd of solution and soil culture, the shoot Cd and grain Cd, but no correlation between the root Cd and grain Cd. GWA mapping detected 9 quantitative trait loci (QTL) for root Cd, 21 for shoot Cd, 14 for root-to-shoot translocation and 15 for grain Cd. A common QTL for the shoot Cd and root-to-shoot translocation was found at 132.6 cM on chromosome 5H. Two major QTL for grain Cd were identified on chromosome 2H and chromosome 5H.The genetic variation in Cd accumulation and major QTL detected provide useful information helpful for cloning candidate genes for Cd accumulation and breeding low-Cd barley cultivars in future.

    DOI: 10.1111/nph.13512

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  • Effect of in planta phosphorus on aluminum-induced inhibition of root elongation in wheat

    Ji Feng Shao, Jing Che, Rong Fu Chen, Jian Feng Ma, Ren Fang Shen

    PLANT AND SOIL395 ( 1-2 ) 307 - 315   2015年10月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Both aluminum (Al) toxicity and phosphorus (P) deficiency are limiting factors of crop production on acid soils. Although Al-P interaction has been extensively studied, the results are controversial. The aim of this study is to investigate the effect of in planta P on Al-induced inhibition of root elongation in wheat (Triticum aestivum L.).Roots of wheat (cv. Atlas 66) with different internal P concentrations were prepared by two methods; split-root and re-rooting in a hydroponic solution using three different P levels (0, 25 and 250 mu M) to avoid direct precipitation of Al-P in the solution. Al toxicity was evaluated by root elongation inhibition and callose induction. The Al and P concentrations in the root tips were also compared among different treatments.Both split-root and re-rooting methods generated roots with different P concentrations in the tips when exposed to different P levels. Lower P in the root tips resulted in less Al-induced inhibition of the root elongation, less callose content and less Al accumulation, while higher root P caused a higher Al-induced inhibition of the root elongation, increased callose content and Al accumulation in the root tips. Furthermore, Al in the root cell sap was not altered by different P concentrations, but Al in the root cell wall was increased with increasing in planta P concentrations.Al toxicity in wheat is associated with P in the root cell wall; lower root P enhanced Al tolerance, while higher root P aggravated Al toxicity in wheat.

    DOI: 10.1007/s11104-015-2566-6

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  • A node-localized transporter OsZIP3 is responsible for the preferential distribution of Zn to developing tissues in rice

    Akimasa Sasaki, Naoki Yamaji, Namiki Mitani-Ueno, Miho Kashino, Jian Feng Ma

    PLANT JOURNAL84 ( 2 ) 374 - 384   2015年10月

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    記述言語:英語   出版者・発行元:WILEY  

    Developing tissues such as meristem with low transpiration require high Zn levels for their active growth, but the molecular mechanisms underlying the preferential distribution to these tissues are poorly understood. We found that a member of the ZIP (ZRT, IRT-like protein), OsZIP3, showed high expression in the nodes of rice (Oryza sativa). Immunostaining revealed that OsZIP3 was localized at the xylem intervening parenchyma cells and xylem transfer cells of the enlarged vascular bundle in both basal and upper nodes. Neither OsZIP3 gene expression nor encoded protein was affected by either deficiency or toxic levels of Zn. Knockdown of OsZIP3 resulted in significantly reduced Zn levels in the shoot basal region containing the shoot meristem and elongating zone, but increased Zn levels in the transpiration flow. A short-term experiment with the Zn-67 stable isotope showed that more Zn was distributed to the lower leaves, but less to the shoot elongating zone and nodes in the knockdown lines compared with the wild-type rice at both the vegetative and reproductive growth stages. Taken together, OsZIP3 located in the node is responsible for unloading Zn from the xylem of enlarged vascular bundles, which is the first step for preferential distribution of Zn to the developing tissues in rice.Significance Statement Zinc homeostasis is achieved by regulating diverse zinc transporters. Here we show that the zinc transporter OsZIP3 mediates the first step leading to preferential distribution of zinc in developing tissues, by unloading zinc from the xylem of enlarged vascular bundles.

    DOI: 10.1111/tpj.13005

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  • Orchestration of three transporters and distinct vascular structures in node for intervascular transfer of silicon in rice

    Naoki Yamaji, Gen Sakurai, Namiki Mitani-Ueno, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA112 ( 36 ) 11401 - 11406   2015年9月

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    記述言語:英語   出版者・発行元:NATL ACAD SCIENCES  

    Requirement of mineral elements in different plant tissues is not often consistent with their transpiration rate; therefore, plants have developed systems for preferential distribution of mineral elements to the developing tissues with low transpiration. Here we took silicon (Si) as an example and revealed an efficient system for preferential distribution of Si in the node of rice (Oryza sativa). Rice is able to accumulate more than 10% Si of the dry weight in the husk, which is required for protecting the grains from water loss and pathogen infection. However, it has been unknown for a long time how this hyperaccumulation is achieved. We found that three transporters (Lsi2, Lsi3, and Lsi6) located at the node are involved in the intervascular transfer, which is required for the preferential distribution of Si. Lsi2 was polarly localized to the bundle sheath cell layer around the enlarged vascular bundles, which is next to the xylem transfer cell layer where Lsi6 is localized. Lsi3 was located in the parenchyma tissues between enlarged vascular bundles and diffuse vascular bundles. Similar to Lsi6, knockout of Lsi2 and Lsi3 also resulted in decreased distribution of Si to the panicles but increased Si to the flag leaf. Furthermore, we constructed a mathematical model for Si distribution and revealed that in addition to cooperation of three transporters, an apoplastic barrier localized at the bundle sheath cells and development of the enlarged vascular bundles in node are also required for the hyperaccumulation of Si in rice husk.

    DOI: 10.1073/pnas.1508987112

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  • Improving Nitrogen Use Efficiency in Rice through Enhancing Root Nitrate Uptake Mediated by a Nitrate Transporter, NRT1.1B

    Zhi Chang Chen, Jian Feng Ma

    JOURNAL OF GENETICS AND GENOMICS42 ( 9 ) 463 - 465   2015年9月

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    記述言語:英語   出版者・発行元:SCIENCE PRESS  

    DOI: 10.1016/j.jgg.2015.08.003

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  • Altered cell wall properties are responsible for ammonium-reduced aluminium accumulation in rice roots

    Wei Wang, Xue Qiang Zhao, Rong Fu Chen, Xiao Ying Dong, Ping Lan, Jian Feng Ma, Ren Fang Shen

    PLANT CELL AND ENVIRONMENT38 ( 7 ) 1382 - 1390   2015年7月

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    記述言語:英語   出版者・発行元:WILEY  

    The phytotoxicity of aluminium (Al) ions can be alleviated by ammonium (NH4+) in rice and this effect has been attributed to the decreased Al accumulation in the roots. Here, the effects of different nitrogen forms on cell wall properties were compared in two rice cultivars differing in Al tolerance. An in vitroAl-binding assay revealed that neither NH4+ nor NO3- altered the Al-binding capacity of cell walls, which were extracted from plants not previously exposed to N sources. However, cell walls extracted from NH4+-supplied roots displayed lower Al-binding capacity than those from NO3--supplied roots when grown in non-buffered solutions. Fourier-transform infrared microspectroscopy analysis revealed that, compared with NO3--supplied roots, NH4+-supplied roots possessed fewer Al-binding groups (-OH and COO-) and lower contents of pectin and hemicellulose. However, when grown in pH-buffered solutions, these differences in the cell wall properties were not observed. Further analysis showed that the Al-binding capacity and properties of cell walls were also altered by pHs alone. Taken together, our results indicate that the NH4+-reduced Al accumulation was attributed to the altered cell wall properties triggered by pH decrease due to NH4+ uptake rather than direct competition for the cell wall binding sites between Al3+ and NH4+.Ammonium (NH4+) is known to alleviate Al toxicity by reducing Al accumulation in rice roots, but the responsible mechanisms are poorly understood. In this study, we found that the NH4+-reduced accumulation of Al was associated with decreased Al-binding groups (-OH and COO-) and contents of pectin and hemicellulose in both Al-tolerant and Al-sensitive rice cultivars. Furthermore, these alterations of cell wall properties were caused by pH decrease due to uptake of NH4+.

    DOI: 10.1111/pce.12490

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  • A cooperative system of silicon transport in plants

    Jian Feng Ma, Naoki Yamaji

    TRENDS IN PLANT SCIENCE20 ( 7 ) 435 - 442   2015年7月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:ELSEVIER SCIENCE LONDON  

    The high accumulation of silicon (Si) protects plants from biotic and abiotic stresses. Two different types of Si transporter [Low Silicon 1 (Lsi1) and 2 (Lsi2)] involved in the uptake and distribution of Si have been identified. Lsi1, a Si permeable channel, belongs to the Nod26-like major intrinsic protein (NIP) III subgroup of the aquaporin membrane protein family with a distinct selectivity, whereas Lsi2, an efflux Si transporter, belongs to an uncharacterized anion transporter family. These transporters are localized to the plasma membrane, but, in different plant species, show different expression patterns and tissue or cellular localizations that are associated with different levels of Si accumulation. A recent mathematical modeling study revealed that cooperation of Lsi1 and Lsi2, which show a polarized localization, is required for the efficient transport of Si in rice.

    DOI: 10.1016/j.tplants.2015.04.007

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  • The role of nodes in arsenic storage and distribution in rice

    Yi Chen, Katie L. Moore, Anthony J. Miller, Steve P. McGrath, Jian Feng Ma, Fang-Jie Zhao

    JOURNAL OF EXPERIMENTAL BOTANY66 ( 13 ) 3717 - 3724   2015年7月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Knowledge of arsenic (As) accumulation in rice (Oryza sativa L.) is important for minimizing As transfer to the food chain. The aim of this study was to investigate the role of rice nodes in As storage and distribution. Synchrotron mu X-ray fluorescence (mu-XRF) was used to map As distribution in the top node and internode of a lsi2 mutant defective in silicon/arsenite efflux carrier and its wild-type (WT) grown in soil. Lsi2 expression in different tissues during grain filling was investigated by quantitative RT-PCR. Arsenite or dimethylarsinic acid (DMA) was supplied to excised panicles to investigate the roles of Lsi2 and phytochelatins (PC) in As distribution. mu-XRF mapping revealed As storage in the phloem of different vascular bundles in the top node and internode. Soil-grown plants of lsi2 had markedly decreased As accumulation in the phloem compared with the WT. Lsi2 was strongly expressed, not only in the roots but also in the nodes. When excised panicles were exposed to As(III), the lsi2 mutant distributed more As to the node and flag leaf but less As to the grain compared with the WT, while there was no significant difference in DMA distribution. Inhibition of PC synthesis by L-buthionine-sulphoximine decreased As(III) deposition in the top node but increased As accumulation in the grain and flag leaf. The results suggest that rice nodes serve as a filter restricting As(III) distribution to the grain. Furthermore, Lsi2 plays a role in As(III) distribution in rice nodes and phytochelatins are important compounds for As(III) storage in the nodes.

    DOI: 10.1093/jxb/erv164

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  • 葉緑体ビタミンCトランスポーターの同定とその生理的役割

    宮地孝明, 黒森崇, 竹内優, 山地直樹, 横正健剛, 嶋澤厚, 杉本絵理子, 表弘志, 馬建鋒, 篠崎一雄, 森山芳則, 森山芳則

    トランスポーター研究会年会抄録集10th   41   2015年6月

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    記述言語:日本語  

    J-GLOBAL

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  • In Silico Simulation Modeling Reveals the Importance of the Casparian Strip for Efficient Silicon Uptake in Rice Roots

    Gen Sakurai, Akiko Satake, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Francois Gabriel Feugier, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY56 ( 4 ) 631 - 639   2015年4月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Silicon (Si) uptake by the roots is mediated by two different transporters, Lsi1 (passive) and Lsi2 (active), in rice (Oryza sativa). Both transporters are polarly localized in the plasma membranes of exodermal (outer) and endodermal (inner) cells with Casparian strips. However, it is unknown how rice is able to take up large amounts of Si compared with other plants, and why rice Si transporters have a characteristic cellular localization pattern. To answer these questions, we simulated Si uptake by rice roots by developing a mathematical model based on a simple diffusion equation that also accounts for active transport by Lsi2. In this model, we calibrated the model parameters using in vivo experimental data on the Si concentrations in the xylem sap and a Monte Carlo method. In our simulation experiments, we compared the Si uptake between roots with various transporter and Casparian strip locations and estimated the Si transport efficiency of roots with different localization patterns and quantities of the Lsi transporters. We found that the Si uptake by roots that lacked Casparian strips was lower than that of normal roots. This suggests that the doublelayer structure of the Casparian strips is an important factor in the high Si uptake by rice. We also found that among various possible localization patterns, the most efficient one was that of the wild-type rice; this may explain the high Si uptake capacity of rice.

    DOI: 10.1093/pcp/pcv017

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  • The Rice CK2 Kinase Regulates Trafficking of Phosphate Transporters in Response to Phosphate Levels

    Jieyu Chen, Yifeng Wang, Fei Wang, Jian Yang, Mingxing Gao, Changying Li, Yingyao Liu, Yu Liu, Naoki Yamaji, Jian Feng Ma, Javier Paz-Ares, Laurent Nussaume, Shuqun Zhang, Keke Yi, Zhongchang Wu, Ping Wu

    PLANT CELL27 ( 3 ) 711 - 723   2015年3月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Phosphate transporters (PTs) mediate phosphorus uptake and are regulated at the transcriptional and posttranslational levels. In one key mechanism of posttranslational regulation, phosphorylation of PTs affects their trafficking from the endoplasmic reticulum (ER) to the plasma membrane. However, the kinase(s) mediating PT phosphorylation and the mechanism leading to ER retention of phosphorylated PTs remain unclear. In this study, we identified a rice (Oryza sativa) kinase subunit, CK2 beta 3, which interacts with PT2 and PT8 in a yeast two-hybrid screen. Also, the CK2 alpha 3/beta 3 holoenzyme phosphorylates PT8 under phosphate-sufficient conditions. This phosphorylation inhibited the interaction of PT8 with PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1, a key cofactor regulating the exit of PTs from the ER to the plasma membrane. Additionally, phosphorus starvation promoted CK2 beta 3 degradation, relieving the negative regulation of PT phosphorus-insufficient conditions. In accordance, transgenic expression of a nonphosphorylatable version of OsPT8 resulted in elevated levels of that protein at the plasma membrane and enhanced phosphorus accumulation and plant growth under various phosphorus regimes. Taken together, these results indicate that CK2 alpha 3/beta 3 negatively regulates PTs and phosphorus status regulates CK2 alpha 3/beta 3.

    DOI: 10.1105/tpc.114.135335

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  • Exploring the power of plants to overcome environmental stresses

    Motoyuki Ashikari, Jian Feng Ma

    RICE8   10   2015年2月

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    記述言語:英語   出版者・発行元:SPRINGER  

    DOI: 10.1186/s12284-014-0037-y

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  • A small RNA mediated regulation of a stress-activated retrotransposon and the tissue specific transposition during the reproductive period in Arabidopsis

    Wataru Matsunaga, Naohiko Ohama, Noriaki Tanabe, Yukari Masuta, Seiji Masuda, Namiki Mitani, Kazuko Yamaguchi-Shinozaki, Jian F. Ma, Atsushi Kato, Hidetaka Ito

    FRONTIERS IN PLANT SCIENCE6   48   2015年2月

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    記述言語:英語   出版者・発行元:FRONTIERS RESEARCH FOUNDATION  

    Transposable elements (TEs) are key elements that facilitate genome evolution of the host organism. A number of studies have assessed the functions of TEs, which change gene expression in the host genome. Activation of TEs is controlled by epigenetic modifications such as DNA methylation and histone modifications. Several recent studies have reported that TEs can also be activated by biotic or abiotic stress in some plants. We focused on a Ty1/copia retrotransposon, ONSEN, that is activated by heat stress (HS) in Arabidopsis. We found that transcriptional activation of ONSEN was regulated by a small interfering RNA (siRNA)-related pathway, and the activation could also be induced by oxidative stress. Mutants deficient in siRNA biogenesis that were exposed to HS at the initial stages of vegetative growth showed transgenerational transposition. The transposition was also detected in the progeny, which originated from tissue that had differentiated after exposure to the HS. The results indicated that in some undifferentiated cells, transpositional activity could be maintained quite long after exposure to the HS.

    DOI: 10.3389/fpls.2015.00048

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  • AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis

    Takaaki Miyaji, Takashi Kuromori, Yu Takeuchi, Naoki Yamaji, Kengo Yokosho, Atsushi Shimazawa, Eriko Sugimoto, Hiroshi Omote, Jian Feng Ma, Kazuo Shinozaki, Yoshinori Moriyama

    NATURE COMMUNICATIONS6   5928   2015年1月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4; 4 protein exhibit membrane potential- and Cl- dependent ascorbate uptake. The AtPHT4; 4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4; 4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4; 4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress.

    DOI: 10.1038/ncomms6928

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  • Physiological characterization of aluminum tolerance and accumulation in tartary and wild buckwheat

    Hua Wang, Rong Fu Chen, Takashi Iwashita, Ren Fang Shen, Jian Feng Ma

    NEW PHYTOLOGIST205 ( 1 ) 273 - 279   2015年1月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Ionic aluminum (Al) is toxic for plant growth, but some plant species are able to accumulate Al at high concentrations without showing toxicity symptoms. In order to determine whether other species in the genus Fagopyrum are able to accumulate Al like common buckwheat (Fagopyrum esculentum), we investigated the external and internal detoxification mechanisms of Al in two self-compatible species: tartary (Fagopyrum tataricum) and wild buckwheat (Fagopyrum homotropicum). Both tartary and wild buckwheat showed high Al tolerance comparable to common buckwheat. Furthermore, these two species also secreted oxalate rapidly from the roots in response to Al in a time-dependent manner. Both tartary and wild buckwheat accumulated >1mgg(-1) Al in the leaves after short-term exposure to Al. Analysis with Al-27-nuclear magnetic resonance (NMR) revealed that Al was present in the form of Al-oxalate (1:3 ratio) in the roots and leaves, but in the form of Al-citrate (1:1 ratio) in the xylem sap in both species. These results indicate that similar to common buckwheat, both tartary and wild buckwheat detoxify Al externally and internally, respectively, by secreting oxalate from the roots and by forming the Al-oxalate complex, which is a nonphytotoxic form. These features of Al response and accumulation may be conserved in genus Fagopyrum.

    DOI: 10.1111/nph.13011

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  • 「新学術領域研究「大地環境変動に対する植物の生存・成長」

    馬 建鋒

    日本数理生物学会ニュースレター76   1 - 2   2015年

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  • 酸性土壌を突破する植物の戦略

    山地直樹, 馬 建鋒

    化学と生物53   529 - 534   2015年

  • 酸性土壌を突破する植物の戦略

    山地直樹, 馬 建鋒

    化学と生物53   529 - 534   2015年

  • 「新学術領域研究「大地環境変動に対する植物の生存・成長」

    馬 建鋒

    日本数理生物学会ニュースレター76   1 - 2   2015年

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  • 4-1-1 イネ節におけるケイ酸分配機構の統合解析(4-1 植物の多量栄養素,2015年度京都大会)

    山地 直樹, 櫻井 玄, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集61 ( 0 )   2015年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P4-3-11 アルミニウム処理下のソバの細胞膜と液胞膜のプロテオーム解析(ポスター,4-3 植物の有害元素,2015年度京都大会)

    横正 健剛, 雷 貴傑, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集61 ( 0 )   2015年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 4-3-15 Characterization of two Al-induced half-size ABC transporters, FeALS1 with different size in buckwheat

    雷 貴傑, 横正 健剛, 山地 直樹, 柏野 美帆, 馬 建鋒

    日本土壌肥料学会講演要旨集61 ( 0 )   2015年

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    記述言語:英語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 葉緑体のアスコルビン酸トランスポーターの同定とその生理的役割

    宮地孝明, 黒森崇, 竹内優, 山地直樹, 横正健剛, 嶋澤厚, 杉本絵理子, 表弘志, 馬建鋒, 篠崎一雄, 森山芳則

    日本薬学会年会要旨集(CD-ROM)135th   ROMBUNNO.26P-PM12   2015年

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    記述言語:日本語  

    J-GLOBAL

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  • Global Transcriptome Analysis of Al-Induced Genes in an Al-Accumulating Species, Common Buckwheat (Fagopyrum esculentum Moench)

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY55 ( 12 ) 2077 - 2091   2014年12月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Buckwheat (Fagopyrum esculentum Moench) is a species with high aluminum (Al) tolerance and accumulation. Although the physiological mechanisms for external and internal detoxification of Al have been well studied, the molecular mechanisms responsible are poorly understood. Here, we conducted a genome-wide transcriptome analysis of Al-responsive genes in the roots and leaves using RNA sequencing (RNA-Seq) technology. RNA-Seq generated reads ranging from 56 x 10(6) to 93 x 10(6). A total of 148,734 transcript contigs with an average length of 1,014 bp were assembled, generating 84,516 unigenes. Among them, 31,730 and 23,853 unigenes were annotated, respectively, in the NCBI plant database and TAIR database for Arabidopsis. Of the annotated genes, 4,067 genes in the roots and 2,663 genes in the leaves were up-regulated (>2-fold) by Al exposure, while 2,456 genes in the roots and 2,426 genes in the leaves were down-regulated (<2-fold) A few STOP1/ART1 (SENSITIVE TO PROTON RHIZOTOXICITY1/AL RESISTANCE TRANSCRIPTION FACTOR1)-regulated gene homologs including FeSTAR1, FeALS3 (ALUMINUM SENSITIVE3), FeALS1 (ALUMINUM SENSITIVE1), FeMATE1 and FeMATE2 (MULTIDRUG AND TOXIC COMPOUND EXTRUSION1 and 2) were also up-regulated in buckwheat, indicating some common Al tolerance mechanism across the species, although most STOP1/ART1-regulated gene homologs were not changed. Most genes involved in citric and oxalic acid biosynthesis were not significantly altered. Some transporter genes were highly expressed in the roots and leaves and responded to Al stress, implicating their role in Al tolerance and accumulation. Overall, our data provide a platform for further characterizing the functions of genes involved in Al tolerance and accumulation in buckwheat.

    DOI: 10.1093/pcp/pcu135

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  • Transcriptional activity and silencing of heat activated retrotransposon in Arabidopsis

    Wataru Matsunaga, Yukari Masuta, Namiki Mitani, Ma Jian Feng, Atsushi Kato, Hidetaka Ito

    GENES & GENETIC SYSTEMS89 ( 6 ) 316 - 316   2014年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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  • Overexpression of OsHMA3 enhances Cd tolerance and expression of Zn transporter genes in rice

    Akimasa Sasaki, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY65 ( 20 ) 6013 - 6021   2014年11月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    As a member of the heavy metal ATPase (HMA) family, OsHMA3 is a tonoplast-localized transporter for Cd in the roots of rice (Oryza sativa). Overexpression of OsHMA3 selectively reduces Cd accumulation in the grain. Further characterization in the present study revealed that overexpression of OsHMA3 also enhances the tolerance to toxic Cd. The growth of both the roots and shoots was similar in the absence of Cd between an OsHMA3-overexpressed line and vector control, but the Cd-inhibited growth was significantly alleviated in the OsHMA3-overexpressed line. The over-expressed line showed higher Cd concentration in the roots, but lower Cd concentration in the shoots compared with the wild-type rice and vector control line, indicating that overexpression of OsHMA3 enhanced vacuolar sequestration of Cd in the roots. The Zn concentration in the roots of the OsHMA3-overexpressed line was constantly higher than that of vector control, but the Zn concentration in the shoots was similar between the overexpressed line and vector control. Five transporter genes belonging to the ZIP family were constitutively up-regulated in the OsHMA3-overexpressed line. These results suggest that shoot Zn level was maintained by up-regulating these genes involved in the Zn uptake/translocation. Taken together, overexpression of OsHMA3 is an efficient way to reduce Cd accumulation in the grain and to enhance Cd tolerance in rice.

    DOI: 10.1093/jxb/eru340

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  • A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain

    Won-Yong Song, Tomohiro Yamaki, Naoki Yamaji, Donghwi Ko, Ki-Hong Jung, Miho Fujii-Kashino, Gynheung An, Enrico Martinoia, Youngsook Lee, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA111 ( 44 ) 15699 - 15704   2014年11月

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    記述言語:英語   出版者・発行元:NATL ACAD SCIENCES  

    Arsenic (As) is a chronic poison that causes severe skin lesions and cancer. Rice (Oryza sativa L.) is a major dietary source of As; therefore, reducing As accumulation in the rice grain and thereby diminishing the amount of As that enters the food chain is of critical importance. Here, we report that a member of the Oryza sativa C-type ATP-binding cassette (ABC) transporter (OsABCC) family, OsABCC1, is involved in the detoxification and reduction of As in rice grains. We found that OsABCC1 was expressed in many organs, including the roots, leaves, nodes, peduncle, and rachis. Expression was not affected when plants were exposed to low levels of As but was up-regulated in response to high levels of As. In both the basal nodes and upper nodes, which are connected to the panicle, OsABCC1 was localized to the phloem region of vascular bundles. Furthermore, OsABCC1 was localized to the tonoplast and conferred phytochelatin-dependent As resistance in yeast. Knockout of OsABCC1 in rice resulted in decreased tolerance to As, but did not affect cadmium toxicity. At the reproductive growth stage, the As content was higher in the nodes and in other tissues of wild-type rice than in those of OsABCC1 knockout mutants, but was significantly lower in the grain. Taken together, our results indicate that OsABCC1 limits As transport to the grains by sequestering As in the vacuoles of the phloem companion cells of the nodes in rice.

    DOI: 10.1073/pnas.1414968111

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  • The node, a hub for mineral nutrient distribution in graminaceous plants

    Naoki Yamaji, Jian Feng Ma

    TRENDS IN PLANT SCIENCE19 ( 9 ) 556 - 563   2014年9月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:ELSEVIER SCIENCE LONDON  

    Mineral elements, including both essential and toxic elements, are delivered to different tissues after they are taken up from the roots, but the mechanism (or mechanisms) underlying the distribution remains poorly understood. In graminaceous plants, this distribution occurs in nodes, which have a complex, well-organized vascular system. A transfer of mineral elements between different vascular bundles is required, especially for preferential distribution to developing tissues that have low transpiration but high nutrient requirements. This intervascular transfer is mediated by various transporters localized at different cells in the node. In this opinion article, we propose four modes of distribution for different mineral elements: xylem-switch, phloem-tropic, phloem-kickback, and minimum-shift, based on specific molecular transport processes identified in the nodes mainly of rice (Oryza sativa). We also discuss the prospects for future studies on mineral nutrient distribution in the nodes.

    DOI: 10.1016/j.tplants.2014.05.007

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  • Molecular mechanisms of Al tolerance in gramineous plants

    Jian Feng Ma, Zhi Chang Chen, Ren Fang Shen

    PLANT AND SOIL381 ( 1-2 ) 1 - 12   2014年8月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:SPRINGER  

    Aluminum (Al) toxicity has limited the productivity and expansion of cereal crops on acid soils; however, a number of plant species or cultivars have developed different strategies for detoxifying aluminum both internally and externally.This review focuses on recent progress on molecular mechanisms of Al tolerance in gramineous plants.A common mechanism in all gramineous plants is the secretion of organic acid anions (citrate and malate) from the roots. Genes belonging to ALMT (for Aluminum-activated malate transporter) and MATE (Multidrug and toxic compound extrusion) family involved in the secretion have been identified in several plant species; however, different plant species show different gene expression patterns including Al-induction, spatial and temporal expression, and tissue localization. Furthermore, the mechanisms regulating the gene expression also differ with plant species, which are achieved by increased tandem repeated element, increase of copy number, insertion of transposon, or alteration of cis-acting element. In addition to these common Al exclusion mechanisms, rice as a highly Al-tolerant species has developed a number of other mechanisms for detoxification of Al. A transcription factor for Al tolerance ART1 identified in rice regulates at least 30 genes implicated in internal and external detoxification of Al. These multiple genes may contribute to the high Al tolerance of rice. In the future, regulation mechanisms of Al-tolerance genes need to be further investigated.

    DOI: 10.1007/s11104-014-2073-1

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  • Differential expression of Nrat1 is responsible for Al-tolerance QTL on chromosome 2 in rice

    Jixing Xia, Naoki Yamaji, Jing Che, Ren Fang Shen, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY65 ( 15 ) 4297 - 4304   2014年8月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Although rice (Oryza sativa) is the most Al-tolerant species among small-grain cereal crops, there is wide genotypic variation in its tolerance to Al toxicity. A number of quantitative trait loci (QTLs) for Al tolerance have been detected, but the responsible genes have not been identified. By using chromosome segment substitution lines, this work found that Nrat1, a gene encoding an Al transporter, is responsible for a QTL previously detected on chromosome 2. Substitution of the chromosome segment containing Nrat1 from Koshihikari (Al-tolerant variety) by that from Kasalath (Al-sensitive variety) decreased Nrat1 expression and Al uptake and tolerance, but increased binding of Al to the cell wall. Nrat1 in Kasalath showed tissue localization similar to Koshihikari in the roots. Although Koshihikari and Kasalath differed in four amino acids in Nrat1 protein, Nrat1 from Kasalath also showed transport activity for Al. Analysis with site-directed mutagenesis revealed that these differences did not affect the Al-transport activity much. Furthermore, there was no correlation between Al tolerance and the open-reading-frame sequence differences in other rice varieties. On the other hand, there was good correlation between Nrat1 expression and Al tolerance; however, sequence comparison of the promoter region up to 2.1 kb did not give a clear difference between the Al-tolerant and -sensitive varieties. Taken together, these results indicate that differential expression of Nrat1 is responsible for the QTL for Al tolerance on chromosome 2, although the mechanism controlling Nrat1 expression remains to be examined.

    DOI: 10.1093/jxb/eru201

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  • Physiological and molecular characterization of Si uptake in wild rice species

    Namiki Mitani-Ueno, Hisao Ogai, Naoki Yamaji, Jian Feng Ma

    PHYSIOLOGIA PLANTARUM151 ( 3 ) 200 - 207   2014年7月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Cultivated rice (Oryza sativa) accumulates high concentration of silicon (Si), which is required for its high and sustainable production. High Si accumulation in cultivated rice is achieved by a high expression of both influx (Lsi1) and efflux (Lsi2) Si transporters in roots. Herein, we physiologically investigated Si uptake, isolated and functionally characterized Si transporters in six wild rice species with different genome types. Si uptake by the roots was lower in Oryza rufipogon, Oryza barthii (AA genome), Oryza australiensis (EE genome) and Oryza punctata (BB genome), but similar in Oryza glumaepatula and Oryza meridionalis (AA genome) compared with the cultivated rice (cv. Nipponbare). However, all wild rice species and the cultivated rice showed similar concentration of Si in the shoots when grown in a field. All species with AA genome showed the same amino acid sequence of both Lsi1 and Lsi2 as O. sativa, whereas species with EE and BB genome showed several nucleotide differences in both Lsi1 and Lsi2. However, proteins encoded by these genes also showed transport activity for Si in Xenopus oocyte. The mRNA expression of Lsi1 in all wild rice species was lower than that in the cultivated rice, whereas the expression of Lsi2 was lower in O. rufipogon and O. barthii but similar in other species. Similar cellular localization of Lsi1 and Lsi2 was observed in all wild rice as the cultivated rice. These results indicate that superior Si uptake, the important trait for rice growth, is basically conserved in wild and cultivated rice species.

    DOI: 10.1111/ppl.12125

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  • Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation

    Matthew J. Milner, Namiki Mitani-Ueno, Naoki Yamaji, Kengo Yokosho, Eric Craft, Zhangjun Fei, Stephen Ebbs, M. Clemencia Zambrano, Jian Feng Ma, Leon V. Kochian

    PLANT JOURNAL78 ( 3 ) 398 - 410   2014年5月

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    記述言語:英語   出版者・発行元:WILEY  

    The Zn/Cd hyperaccumulator, Noccaea caerulescens, has been studied extensively for its ability to accumulate high levels of Zn and Cd in its leaves. Previous studies have indicated that the Zn and Cd hyperaccumulation trait exhibited by this species involves different transport and tolerance mechanisms. It has also been well documented that certain ecotypes of N.caerulescens are much better Cd hyperaccumulators than others. However, there does not seem to be much ecotypic variation for Zn hyperaccumulation in N.caerulescens. In this study we employed a comparative transcriptomics approach to look at root and shoot gene expression in Ganges and Prayon plants in response to Cd stress to identify transporter genes that were more highly expressed in either the roots or shoots of the superior Cd accumulator, Ganges. Comparison of the transcriptomes from the two ecotypes of Noccaea caerulescens identified a number of genes that encoded metal transporters that were more highly expressed in the Ganges ecotype in response to Cd stress. Characterization of one of these transporters, NcNramp1, showed that it is involved in the influx of Cd across the endodermal plasma membrane and thus may play a key role in Cd flux into the stele and root-to-shoot Cd transport. NcNramp1 may be one of the main transporters involved in Cd hyperaccumulation in N.caerulescens and copy number variation appears to be the main reason for high NcNramp1 gene expression underlying the increased Cd accumulation in the Ganges ecotype.

    DOI: 10.1111/tpj.12480

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  • Normal root elongation requires arginine produced by argininosuccinate lyase in rice

    Jixing Xia, Naoki Yamaji, Jing Che, Ren Fang Shen, Jian Feng Ma

    PLANT JOURNAL78 ( 2 ) 215 - 226   2014年4月

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    記述言語:英語   出版者・発行元:WILEY  

    Plant roots play an important role in the uptake of water and nutrients, structural support and environmental sensing, but the molecular mechanisms involved in root development are poorly understood in rice (Oryza sativa), which is characterized by a dense fibrous root system. Here we report a rice mutant (red1 for root elongation defect 1) with short roots. Morphological and physiological analyses showed that the mutant had a shorter length from the quiescent center (QC) to the starting point of the elongation zone but a similar cell size and number of lateral and crown roots compared with the wild type. Furthermore, the mutant had similar radial structure and nutrient uptake patterns to the wild type. Map-based cloning revealed that the mutant phenotype was caused by a point mutation of a gene encoding an argininosuccinate lyase (ASL), catalyzing the last step of arginine biosynthesis. The OsASL1 gene has two distinct transcripts, OsASL1.1 and OsASL1.2, which result from different transcription start sites, but only OsASL1.1 was able to complement the mutant phenotype. OsASL1.1 was expressed in both the roots and shoots. The protein encoded by OsASL1.1 showed ASL activity in yeast. OsALS1.1 was localized to the plastid. The short root of the mutant was rescued by exogenous addition of arginine, but not by other amino acids. These results indicate that arginine produced by ASL is required for normal root elongation in rice.

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  • Rice grain resistance to brown spot and yield are increased by silicon

    Leandro J. Dallagnol, Fabricio A. Rodrigues, Mateus V. B. Mielli, Jian F. Ma

    TROPICAL PLANT PATHOLOGY39 ( 1 ) 56 - 63   2014年1月

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    記述言語:英語   出版者・発行元:SOC BRASILEIRA FITOPATHOLOGIA  

    Brown spot, caused by Bipolaris oryzae, is one of the most important diseases of rice and can cause a reduction in yield and grain quality. The effect of silicon (Si) on the resistance of rice grains to brown spot was investigated. Plants from cv. Oochikara and its mutant, defective in the Lsi1 transporter (lsi1 mutant), were grown in hydroponic culture either with Si (+ Si; 2 mM) or without Si (-Si). Panicle inoculation with B. oryzae was carried out at the beginning of the milk-grain stage. Panicles were harvested at physiological grain maturity. The supply of Si significantly increased Si concentration in husks compared to -Si plants. Si concentration in husks from cv. Oochikara was up to three times greater than the lsi1 mutant. In the presence of Si, brown spot severity was reduced by 88% in grains from cv. Oochikara and by 53% in grains from lsi1 mutant. Brown spot severity was 77% lower for grains of cv. Oochikara than for the lsi1 mutant, both plant types were grown in the presence of Si. Panicle inoculation reduced significantly the following yield components: number of grains per panicle, the weight of 1000 grains and the percentage of filled grains. Si significantly increased these yield components, especially for inoculated panicles. Considering kernel quality, the panicle inoculation with B. oryzae significantly reduced the yield of husked kernel, yield of whole kernel and kernel diameter, especially for grains from -Si plants. For panicles from + Si plants, the kernel quality was improved under inoculation, compared to -Si plants. Results from this study show that Si improved rice yield and kernel quality in panicles inoculated with B. oryzae. Furthermore the functional Lsi1 gene contributed significantly for increasing the yield of whole kernel and kernel diameter, possibly due to the increasing Si concentration in husks.

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  • An appropriate concentration of arginine is required for normal root growth in rice

    Jixing Xia, Naoki Yamaji, Jian Feng Ma

    Plant Signaling and Behavior9   e28717   2014年

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    記述言語:英語   出版者・発行元:Landes Bioscience  

    Plant roots play an important role in uptake of water and nutrients, support of above-ground part and environmental sensing, but the molecular mechanisms underlying the root development are poorly understood in rice. We found that a gene (OsASL1) encoding argininosuccinate lyase is involved in normal root development of rice. OsASL1 cleaves argininosuccinate to arginine and fumarate reversibly, the last step in the arginine biosynthetic pathway. Here, we further characterized OsASL1 in terms of expression pattern, subcellular localization, and arginine effect on the root growth. A detailed expression analysis revealed that 2 transcripts of OsASL1, OsASL1.1 and OsASL1.2, showed different expression patterns
    OsASL1.1 was expressed in most organs throughout the whole growth period, whereas OsASL1.2 was mainly expressed in the roots. In contrast to plastid-localized OsASL1.1, OsASL1.2 was localized to the cytosol and nucleus. The short-root phenotype of the mutant was not rescued by exogenous addition of the sodium nitroprusside, a nitric oxide donor, but rescued by an appropriate concentration of Arg. Our results indicate that the subcellular localization was determined by the N terminus of OsASL1 and that appropriate concentration of Arg is required for normal root elongation in rice. © 2014 Landes Bioscience.

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  • 1-5 イネにおけるケイ素吸収・輸送モデルによる植物体構造とその意義の解明(I 植物栄養と数理モデルの接点-数理モデルで植物栄養の仕組を理解する,シンポジウム,2014年度東京大会)

    櫻井 玄, 佐竹 暁子, 山地 直樹, 三谷 奈見季, 横沢 正幸, 馬 建鋒

    日本土壌肥料学会講演要旨集60 ( 0 )   2014年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 4-3-17 アルミニウム集積植物ソバのAl応答遺伝子の網羅的発現解析(4-3 植物の有害元素,2014年度東京大会)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集60 ( 0 )   2014年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 8 クエン酸輸送体OsFRDL1はイネの地上部の鉄分配にも関与する(関西支部講演会,2013年度各支部会)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集60 ( 0 )   2014年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 10 イネ野生種におけるケイ酸吸収機構に関する研究(関西支部講演会,2013年度各支部会)

    三谷 奈見季, 大貝 久夫, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集60 ( 0 )   2014年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 14 イネのマンガン集積に関与する輸送体OsMTP8の解析(関西支部講演会,2013年度各支部会)

    竹本 侑馬, 三谷 奈見季, 佐々木 明正, 山地 直樹, 馬 建鋒, 加藤 伸一郎, 岩崎 貢三, 上野 大勢

    日本土壌肥料学会講演要旨集60 ( 0 )   2014年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family

    Zonghui Chen, Yumi Fujii, Naoki Yamaji, Sakine Masuda, Yuma Takemoto, Takehiro Kamiya, Yusufujiang Yusuyin, Kozo Iwasaki, Shin-ichiro Kato, Masayoshi Maeshima, Jian Feng Ma, Daisei Ueno

    JOURNAL OF EXPERIMENTAL BOTANY64 ( 14 ) 4375 - 4387   2013年11月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Manganese (Mn) is an essential micronutrient for plants, but is toxic when present in excess. The rice plant (Oryza sativa L.) accumulates high concentrations of Mn in the aerial parts; however, the molecular basis for Mn tolerance is poorly understood. In the present study, genes encoding Mn tolerance were screened for by expressing cDNAs of genes from rice shoots in Saccharomyces cerevisiae. A gene encoding a cation diffusion facilitator (CDF) family member, OsMTP8.1, was isolated, and its expression was found to enhance Mn accumulation and tolerance in S. cerevisiae. In plants, OsMTP8.1 and its transcript were mainly detected in shoots. High or low supply of Mn moderately induced an increase or decrease in the accumulation of OsMTP8.1, respectively. OsMTP8.1 was detected in all cells of leaf blades through immunohistochemistry. OsMTP8.1 fused to green fluorescent protein was localized to the tonoplast. Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots. However, there was no difference in the accumulation of other metals, including Zn, Cu, Fe, Mg, Ca, and K. These results suggest that OsMTP8.1 is an Mn-specific transporter that sequesters Mn into vacuoles in rice and is required for Mn tolerance in shoots.

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  • A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

    Fenglin Deng, Naoki Yamaji, Jixing Xia, Jian Feng Ma

    PLANT PHYSIOLOGY163 ( 3 ) 1353 - 1362   2013年11月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Heavy metal-transporting P-type ATPase (HMA) has been implicated in the transport of heavy metals in plants. Here, we report the function and role of an uncharacterized member of HMA, OsHMA5 in rice (Oryza sativa). Knockout of OsHMA5 resulted in a decreased copper (Cu) concentration in the shoots but an increased Cu concentration in the roots at the vegetative stage. At the reproductive stage, the concentration of Cu in the brown rice was significantly lower in the mutants than in the wild-type rice; however, there was no difference in the concentrations of iron, manganese, and zinc between two independent mutants and the wild type. The Cu concentration of xylem sap was lower in the mutants than in the wild-type rice. OsHMA5 was mainly expressed in the roots at the vegetative stage but also in nodes, peduncle, rachis, and husk at the reproductive stage. The expression was up-regulated by excess Cu but not by the deficiency of Cu and other metals, including zinc, iron, and manganese, at the vegetative stage. Analysis of the transgenic rice carrying the OsHMA5 promoter fused with green fluorescent protein revealed that it was localized at the root pericycle cells and xylem region of diffuse vascular bundles in node I, vascular tissues of peduncle, rachis, and husk. Furthermore, immunostaining with an antibody against OsHMA5 revealed that it was localized to the plasma membrane. Expression of OsHMA5 in a Cu transport-defective mutant yeast (Saccharomyces cerevisiae) strain restored the growth. Taken together, OsHMA5 is involved in loading Cu to the xylem of the roots and other organs.

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  • A plasma membrane-localized small peptide is involved in rice aluminum tolerance

    Jixing Xia, Naoki Yamaji, Jian Feng Ma

    PLANT JOURNAL76 ( 2 ) 345 - 355   2013年10月

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    記述言語:英語   出版者・発行元:WILEY  

    A transcription factor for Al tolerance, ART1, regulates the expression of at least 30 genes in rice. Here we functionally characterized one of the downstream genes, OsCDT3, which encodes a predicted peptide of only 53 amino acid residues rich in cysteine. Knockdown of this gene resulted in decreased tolerance to Al, but did not affect the tolerance to Cd. The aluminum (Al) content in the root residues including cell wall and the plasma membrane of knockdown lines decreased, but the Al concentration in the root cell sap increased compared with those of the wild-type rice. OsCDT3 was mainly expressed in the roots and its expression was specifically induced by Al exposure, not by low pH and other metals. There was a small genotypic variation in OsCDT3 expression level, but no correlation between Al tolerance and the OsCDT3 variation was found among 17 rice cultivars. Analysis of pOsCDT3::GFP transgenic rice showed that OsCDT3 was expressed at all cells in the root tips. Transient expression of OsCDT3 fused with GFP at both N- and C-termini showed that OsCDT3 was anchored to the plasma membrane. Expression of OsCDT3 in yeast conferred tolerance to Al, but not to Cd. Furthermore, OsCDT3 did not show transport activity for Al in yeast, but was able to directly bind Al in vitro. Taken together, our results indicate that OsCDT3 anchoring to the plasma membrane may play a role in stopping entry of Al into the root cells by binding Al, therefore, contributing to high Al tolerance in rice.

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  • Adaptation to acidic soil is achieved by increased numbers of cis-acting elements regulating ALMT1 expression in Holcus lanatus

    Zhi Chang Chen, Kengo Yokosho, Miho Kashino, Fang-Jie Zhao, Naoki Yamaji, Jian Feng Ma

    PLANT JOURNAL76 ( 1 ) 10 - 23   2013年10月

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    記述言語:英語   出版者・発行元:WILEY  

    Yorkshire fog (Holcus lanatus), which belongs to the Poaceae family and is a close relative of the agronomic crop oat (Avena sativa), is a widely adaptable grass species that is able to grow on highly acidic soils with high levels of Al, but the mechanism underlying the high Al tolerance is unknown. Here, we characterized two accessions of H.lanatus collected from an acid plot (soil pH 3.6, HL-A) and a neutral plot (pH 7.1, HL-N) in terms of Al tolerance, organic acid anion secretion and related gene expression. In response to Al (pH 4.5), the HL-A roots secreted approximately twice as much malate as the HL-N roots, but there was no difference in citrate secretion. Cloning of the gene HlALMT1 responsible for malate secretion showed that the encoded amino acid sequence did not differ between two accessions, but the expression level in the outer cell layers of the HL-A roots was twice as high as in the HL-N roots. This difference was not due to the genomic copy number, but was due to the number of cis-acting elements for an Al-responsive transcription factor (HlART1) in the promoter region of HlALMT1, as demonstrated by both a yeast one-hybrid assay and a transient assay in tobacco protoplasts. Furthermore, introduction of HlALMT1 driven by the HL-A promoter into rice resulted in significantly more Al-induced malate secretion than introduction of HlALMT1 driven by the HL-N promoter. These findings indicate that the adaptation of H.lanatus to acidic soils may be achieved by increasing number of cis-acting elements for ART1 in the promoter region of the HlALMT1 gene, enhancing the expression of HlALMT1 and the secretion of malate.

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  • A node-based switch for preferential distribution of manganese in rice

    Naoki Yamaji, Akimasa Sasaki, Ji Xing Xia, Kengo Yokosho, Jian Feng Ma

    NATURE COMMUNICATIONS4   2442   2013年9月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Mineral nutrients, such as manganese, are required for the development of plants and their reproductive organs, but these can be toxic if accumulated at high concentrations. Therefore, plants must have a system for preferentially delivering an adequate amount of minerals to these organs for active growth and development, while preventing mineral overaccumulation in the face of changing environments. Here we show that a member of the Nramp transporter family, OsNramp3, functions as a switch in response to environmental Mn changes. OsNramp3 is constitutively expressed in the node, a junction of vasculatures connecting leaves, stems and panicles. At low Mn concentration, OsNramp3 preferentially transports Mn to young leaves and panicles. However, at high Mn concentration, the OsNramp3 protein is rapidly degraded within a few hours, resulting in the distribution of Mn to old tissues. Our results reveal the OsNramp3-mediated strategy of rice for adapting to a wide change of Mn in the environment.

    DOI: 10.1038/ncomms3442

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  • TWIN SISTER OF FT, GIGANTEA, and CONSTANS Have a Positive But Indirect Effect on Blue Light-Induced Stomatal Opening in Arabidopsis

    Eigo Ando, Masato Ohnishi, Yin Wang, Tomonao Matsushita, Aiko Watanabe, Yuki Hayashi, Miho Fujii, Jian Feng Ma, Shin-ichiro Inoue, Toshinori Kinoshita

    PLANT PHYSIOLOGY162 ( 3 ) 1529 - 1538   2013年7月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    FLOWERING LOCUS T (FT) is the major regulatory component controlling photoperiodic floral transition. It is expressed in guard cells and affects blue light-induced stomatal opening induced by the blue-light receptor phototropins phot1 and phot2. Roles for other flowering regulators in stomatal opening have yet to be determined. We show in Arabidopsis (Arabidopsis thaliana) that TWIN SISTER OF FT (TSF), CONSTANS (CO), and GIGANTEA (GI) provide a positive effect on stomatal opening. TSF, which is the closest homolog of FT, was transcribed in guard cells, and light-induced stomatal opening was repressed in tsf-1, a T-DNA insertion mutant of TSF. Overexpression of TSF in a phot1 phot2 mutant background gave a constitutive open-stomata phenotype. Then, we examined whether CO and GI, which are upstream regulators of FT and TSF in photoperiodic flowering, are involved in stomatal opening. Similar to TSF, light-induced stomatal opening was suppressed in the GI and CO mutants gi-1 and co-1. A constitutive open-stomata phenotype was observed in GI and CO overexpressors with accompanying changes in the transcription of both FT and TSF. In photoperiodic flowering, photoperiod is sensed by photoreceptors such as the cryptochromes cry1 and cry2. We examined stomatal phenotypes in a cry1 cry2 mutant and in CRY2 overexpressors. Light-induced stomatal opening was suppressed in cry1 cry2, and the transcription of FT and TSF was down-regulated. In contrast, the stomata in CRY2 overexpressors opened even in the dark, and FT and TSF transcription was up-regulated. We conclude that the photoperiodic flowering components TSF, GI, and CO positively affect stomatal opening.

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  • Magnesium transporters and their role in Al tolerance in plants

    Zhi Chang Chen, Jian Feng Ma

    PLANT AND SOIL368 ( 1-2 ) 51 - 56   2013年7月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Magnesium (Mg) is an essential macronutrient for plant growth, which has diverse biological functions. However, little is known about the transport system of this nutrient in plants. In the genome of plants such as rice and Arabidopsis, there are homologues of bacterial Mg transporters (CorA) and some of them have been functionally characterized, but the physiological role of these transporters are poorly understood. On the other hand, Mg is able to alleviate Al toxicity in a number of plant species, but the mechanisms underlying this alleviation are not well understood. Recently, this alleviation has been associated with a Mg transporter in rice. In this paper, we present our opinions on Mg transporters, which are required for uptake, translocation, distribution and storage in plants. Possible mechanisms for Mg-mediated alleviation of Al toxicity are also discussed.

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  • Magnesium transporters and their role in Al tolerance in plants

    Zhi Chang Chen, Jian Feng Ma

    PLANT AND SOIL368 ( 1-2 ) 51 - 56   2013年7月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Magnesium (Mg) is an essential macronutrient for plant growth, which has diverse biological functions. However, little is known about the transport system of this nutrient in plants. In the genome of plants such as rice and Arabidopsis, there are homologues of bacterial Mg transporters (CorA) and some of them have been functionally characterized, but the physiological role of these transporters are poorly understood. On the other hand, Mg is able to alleviate Al toxicity in a number of plant species, but the mechanisms underlying this alleviation are not well understood. Recently, this alleviation has been associated with a Mg transporter in rice. In this paper, we present our opinions on Mg transporters, which are required for uptake, translocation, distribution and storage in plants. Possible mechanisms for Mg-mediated alleviation of Al toxicity are also discussed.

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  • Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

    Naoki Yamaji, Jixing Xia, Namiki Mitani-Ueno, Kengo Yokosho, Jian Feng Ma

    PLANT PHYSIOLOGY162 ( 2 ) 927 - 939   2013年6月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Developing tissues such as meristems and reproductive organs require high zinc, but the molecular mechanisms of how zinc taken up by the roots is preferentially delivered to these tissues with low transpiration are unknown. Here, we report that rice (Oryza sativa) heavy metal ATPase2 (OsHMA2), a member of P-type ATPases, is involved in preferential delivery of zinc to the developing tissues in rice. OsHMA2 was mainly expressed in the mature zone of the roots at the vegetative stage, but higher expression was also found in the nodes at the reproductive stage. The expression was unaffected by either zinc deficiency or zinc excess. OsHMA2 was localized at the pericycle of the roots and at the phloem of enlarged and diffuse vascular bundles in the nodes. Heterologous expression of OsHMA2 in yeast (Saccharomyces cerevisiae) showed influx transport activity for zinc as well as cadmium. Two independent Tos17 insertion lines showed decreased zinc concentration in the crown root tips, decreased concentration of zinc and cadmium in the upper nodes and reproductive organs compared with wild-type rice. Furthermore, a short-term labeling experiment with Zn-67 showed that the distribution of zinc to the panicle and uppermost node I was decreased, but that, to the lower nodes, was increased in the two mutants. Taken together, OsHMA2 in the nodes plays an important role in preferential distribution of zinc as well as cadmium through the phloem to the developing tissues.

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  • Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

    Naoki Yamaji, Jixing Xia, Namiki Mitani-Ueno, Kengo Yokosho, Jian Feng Ma

    PLANT PHYSIOLOGY162 ( 2 ) 927 - 939   2013年6月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Developing tissues such as meristems and reproductive organs require high zinc, but the molecular mechanisms of how zinc taken up by the roots is preferentially delivered to these tissues with low transpiration are unknown. Here, we report that rice (Oryza sativa) heavy metal ATPase2 (OsHMA2), a member of P-type ATPases, is involved in preferential delivery of zinc to the developing tissues in rice. OsHMA2 was mainly expressed in the mature zone of the roots at the vegetative stage, but higher expression was also found in the nodes at the reproductive stage. The expression was unaffected by either zinc deficiency or zinc excess. OsHMA2 was localized at the pericycle of the roots and at the phloem of enlarged and diffuse vascular bundles in the nodes. Heterologous expression of OsHMA2 in yeast (Saccharomyces cerevisiae) showed influx transport activity for zinc as well as cadmium. Two independent Tos17 insertion lines showed decreased zinc concentration in the crown root tips, decreased concentration of zinc and cadmium in the upper nodes and reproductive organs compared with wild-type rice. Furthermore, a short-term labeling experiment with Zn-67 showed that the distribution of zinc to the panicle and uppermost node I was decreased, but that, to the lower nodes, was increased in the two mutants. Taken together, OsHMA2 in the nodes plays an important role in preferential distribution of zinc as well as cadmium through the phloem to the developing tissues.

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  • LjMATE1: A citrate transporter responsible for iron supply to the nodule infection zone of lotus japonicus

    Kojiro Takanashi, Kengo Yokosho, Kazuhiko Saeki, Akifumi Sugiyama, Shusei Sato, Satoshi Tabata, Jian Feng Ma, Kazufumi Yazaki

    Plant and Cell Physiology54 ( 4 ) 585 - 594   2013年4月

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    記述言語:英語  

    Symbiotic nitrogen fixation by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. While exchanged molecules imply nitrogen compounds, carbohydrates and also various minerals, knowledge of the molecular basis of plant transporters that mediate those metabolite exchanges is still limited. In this study, we have shown that a multidrug and toxic compound extrusion (MATE) protein, LjMATE1, is specifically induced during nodule formation, which nearly paralleled nodule maturation, in a model legume Lotus japonicus. Reporter gene experiments indicated that the expression of LjMATE1 was restricted to the infection zone of nodules. To characterize the transport function of LjMATE1, we conducted a biochemical analysis using a heterologous expression system, Xenopus oocytes, and found that LjMATE1 is a specific transporter for citrate. The physiological role of LjMATE1 was analyzed after generation of L. japonicus RNA interference (RNAi) lines. One RNAi knock-down line revealed limited growth under nitrogen-deficient conditions with inoculation of rhizobia compared with the controls (the wild type and an RNAi line in which LjMATE1 was not suppressed). It was noteworthy that Fe localization was clearly altered in nodule tissues of the knock-down line. These results strongly suggest that LjMATE1 is a nodule-specific transporter that assists the translocation of Fe from the root to nodules by providing citrate. © 2013 The Author 2013. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

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  • LjMATE1: A Citrate Transporter Responsible for Iron Supply to the Nodule Infection Zone of Lotus japonicus

    Kojiro Takanashi, Kengo Yokosho, Kazuhiko Saeki, Akifumi Sugiyama, Shusei Sato, Satoshi Tabata, Jian Feng Ma, Kazufumi Yazaki

    PLANT AND CELL PHYSIOLOGY54 ( 4 ) 585 - 594   2013年4月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Symbiotic nitrogen fixation by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. While exchanged molecules imply nitrogen compounds, carbohydrates and also various minerals, knowledge of the molecular basis of plant transporters that mediate those metabolite exchanges is still limited. In this study, we have shown that a multidrug and toxic compound extrusion (MATE) protein, LjMATE1, is specifically induced during nodule formation, which nearly paralleled nodule maturation, in a model legume Lotus japonicus. Reporter gene experiments indicated that the expression of LjMATE1 was restricted to the infection zone of nodules. To characterize the transport function of LjMATE1, we conducted a biochemical analysis using a heterologous expression system, Xenopus oocytes, and found that LjMATE1 is a specific transporter for citrate. The physiological role of LjMATE1 was analyzed after generation of L. japonicus RNA interference (RNAi) lines. One RNAi knock-down line revealed limited growth under nitrogen-deficient conditions with inoculation of rhizobia compared with the controls (the wild type and an RNAi line in which LjMATE1 was not suppressed). It was noteworthy that Fe localization was clearly altered in nodule tissues of the knock-down line. These results strongly suggest that LjMATE1 is a nodule-specific transporter that assists the translocation of Fe from the root to nodules by providing citrate.

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  • イネの銅再転流に関わる輸送体OsYSL16の解析

    鄭録慶, 山地直樹, 横正健剛, 馬建鋒

    日本植物生理学会年会要旨集54th   153   2013年3月

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  • Divergence of expression pattern contributed to neofunctionalization of duplicated HD-Zip I transcription factor in barley

    Shun Sakuma, Mohammad Pourkheirandish, Goetz Hensel, Jochen Kumlehn, Nils Stein, Akemi Tagiri, Naoki Yamaji, Jian Feng Ma, Hidenori Sassa, Takato Koba, Takao Komatsuda

    NEW PHYTOLOGIST197 ( 3 ) 939 - 948   2013年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Barley (Hordeum vulgare) spikes are developmentally switched from two-rowed to six-rowed by a single recessive gene, six-rowed spike 1 (vrs1), which encodes a homeodomain-leucine zipper I class transcription factor. Vrs1 is a paralog of HvHox2 and both were generated by duplication of an ancestral gene. HvHox2 is conserved among cereals, whereas Vrs1 acquired its current function during the evolution of barley. It was unclear whether divergence of expression pattern or protein function accounted for the functionalization of Vrs1. Here, we conducted a comparative analysis of protein functions and gene expression between HvHox2 and Vrs1 to clarify the functionalization mechanism. We revealed that the transcriptional activation activity of HvHOX2 and VRS1 was conserved. In situ hybridization analysis showed that HvHox2 is localized in vascular bundles in developing spikes, whereas Vrs1 is expressed exclusively in the pistil, lemma, palea and lodicule of lateral spikelets. The transcript abundance of Vrs1 was &gt; 10-fold greater than that of HvHox2 during the pistil developmental stage, suggesting that the essential function of Vrs1 is to inhibit gynoecial development. We demonstrated the quantitative function of Vrs1 using RNAi transgenic plants and Vrs1 expression variants. Expression analysis of six-rowed spike mutants that are nonallelic to vrs1 showed that Vrs1 expression was up-regulated by Vrs4, whereas HvHox2 expression was not. These data demonstrate that the divergence of gene expression pattern contributed to the neofunctionalization of Vrs1.

    DOI: 10.1111/nph.12068

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  • Divergence of expression pattern contributed to neofunctionalization of duplicated HD-Zip I transcription factor in barley

    Shun Sakuma, Mohammad Pourkheirandish, Goetz Hensel, Jochen Kumlehn, Nils Stein, Akemi Tagiri, Naoki Yamaji, Jian Feng Ma, Hidenori Sassa, Takato Koba, Takao Komatsuda

    NEW PHYTOLOGIST197 ( 3 ) 939 - 948   2013年2月

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    記述言語:英語   出版者・発行元:WILEY  

    Barley (Hordeum vulgare) spikes are developmentally switched from two-rowed to six-rowed by a single recessive gene, six-rowed spike 1 (vrs1), which encodes a homeodomain-leucine zipper I class transcription factor. Vrs1 is a paralog of HvHox2 and both were generated by duplication of an ancestral gene. HvHox2 is conserved among cereals, whereas Vrs1 acquired its current function during the evolution of barley. It was unclear whether divergence of expression pattern or protein function accounted for the functionalization of Vrs1. Here, we conducted a comparative analysis of protein functions and gene expression between HvHox2 and Vrs1 to clarify the functionalization mechanism. We revealed that the transcriptional activation activity of HvHOX2 and VRS1 was conserved. In situ hybridization analysis showed that HvHox2 is localized in vascular bundles in developing spikes, whereas Vrs1 is expressed exclusively in the pistil, lemma, palea and lodicule of lateral spikelets. The transcript abundance of Vrs1 was > 10-fold greater than that of HvHox2 during the pistil developmental stage, suggesting that the essential function of Vrs1 is to inhibit gynoecial development. We demonstrated the quantitative function of Vrs1 using RNAi transgenic plants and Vrs1 expression variants. Expression analysis of six-rowed spike mutants that are nonallelic to vrs1 showed that Vrs1 expression was up-regulated by Vrs4, whereas HvHox2 expression was not. These data demonstrate that the divergence of gene expression pattern contributed to the neofunctionalization of Vrs1.

    DOI: 10.1111/nph.12068

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  • 4-1-2 野生イネ由来ケイ酸トランスポーターの解析(4-1 植物の多量栄養素)

    三谷 奈見季, 大貝 久生, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集59 ( 0 )   2013年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 4-2-4 イネ銅の再転流に関与するトランスポーターの同定(4-2 植物の微量栄養素)

    鄭 録慶, 山地 直樹, 横正 健剛, 馬 建鋒

    日本土壌肥料学会講演要旨集59 ( 0 )   2013年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P4-2-3 イネ地上部の鉄分配に関与するクエン酸トランスポーターOsFRDL1(4-2 植物の微量栄養素)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集59 ( 0 )   2013年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • イネの節におけるミネラルの維管束間輸送と分配制御機構

    山地直樹, 佐々木明正, 鄭録慶, 夏継星, 横正健剛, 三谷奈見季, 馬建鋒

    トランスポーター研究会年会抄録集8th   47   2013年

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  • Functional Characterization of a Silicon Transporter Gene Implicated in Silicon Distribution in Barley

    Naoki Yamaji, Yukako Chiba, Namiki Mitani-Ueno, Jian Feng Ma

    PLANT PHYSIOLOGY160 ( 3 ) 1491 - 1497   2012年11月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Silicon (Si) is a beneficial element for plant growth. In barley (Hordeum vulgare), Si uptake by the roots is mainly mediated by a Si channel, Low Silicon1 (HvLsi1), and an efflux transporter, HvLsi2. However, transporters involved in the distribution of Si in the shoots have not been identified. Here, we report the functional characterization of a homolog of HvLsi1, HvLsi6. HvLsi6 showed permeability for Si and localized to the plasma membrane. At the vegetative growth stage, HvLsi6 was expressed in both the roots and shoots. The expression level was unaffected by Si supply. In the roots, HvLsi6 was localized in epidermis and cortex cells of the tips, while in the leaf blades and sheaths, HvLsi6 was only localized at parenchyma cells of vascular bundles. At the reproductive growth stage, high expression of HvLsi6 was also found in the nodes. HvLsi6 in node I was polarly located at the transfer cells surrounding the enlarged vascular bundles toward the numerous xylem vessels. These results suggest that HvLsi6 is involved in Si uptake in the root tips, xylem unloading of Si in leaf blade and sheath, and intervascular transfer of Si in the nodes. Furthermore, HvLsi2 was found to be localized at the parenchyma cell layer adjacent to the transfer cells with opposite polarity of HvLsi6, suggesting that the coupling of HvLsi6 and HvLsi2 is involved in the intervascular transfer of Si at the nodes. Si translocated via the enlarged vascular bundles is unloaded to the transfer cells by HvLsi6, followed by HvLsi2 to reload Si to the diffuse vascular bundles, which are connected to the upper part of the plant, especially the panicles, the ultimate Si sink.

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  • Effect of silicon deficiency on secondary cell wall synthesis in rice leaf

    Tsuyoshi Yamamoto, Atsuko Nakamura, Hiroaki Iwai, Tadashi Ishii, Jian Feng Ma, Ryusuke Yokoyama, Kazuhiko Nishitani, Shinobu Satoh, Jun Furukawa

    JOURNAL OF PLANT RESEARCH125 ( 6 ) 771 - 779   2012年11月

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    記述言語:英語   出版者・発行元:SPRINGER JAPAN KK  

    Rice (Oryza sativa L.) is a typical Si-accumulating plant and is able to accumulate Si up to > 10 % of shoot dry weight. The cell wall has been reported to become thicker under Si-deficient condition. To clarify the relationship between Si accumulation and cell wall components, the physical properties of, and macromolecular components and Si content in, the pectic, hemicellulosic, and cellulosic fractions prepared from rice seedlings grown in hydroponics with or without 1.5 mM silicic acid were analyzed. In the absence of Si (the -Si condition), leaf blades drooped, but physical properties were enhanced. Sugar content in the cellulosic fraction and lignin content in the total cell wall increased under -Si condition. After histochemical staining, there was an increase in cellulose deposition in short cells and the cell layer just beneath the epidermis in the -Si condition, but no significant change in the pattern of lignin deposition. Expression of the genes involved in secondary cell wall synthesis, OsCesA4, OsCesA7, OsPAL, OsCCR1 and OsCAD6 was up-regulated under -Si condition, but expression of OsCesA1, involved in primary cell wall synthesis, did not increase. These results suggest that an increase in secondary cell wall components occurs in rice leaves to compensate for Si deficiency.

    DOI: 10.1007/s10265-012-0489-3

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  • Comparative Genome-Wide Transcriptional Analysis of Al-Responsive Genes Reveals Novel Al Tolerance Mechanisms in Rice

    Tomokazu Tsutsui, Naoki Yamaji, Chao Feng Huang, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

    PLOS ONE7 ( 10 ) e48197   2012年10月

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    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Rice (Oryza sativa) is the most aluminum (Al)-tolerant crop among small-grain cereals, but the mechanism underlying its high Al resistance is still not well understood. To understand the mechanisms underlying high Al-tolerance, we performed a comparative genome-wide transcriptional analysis by comparing expression profiling between the Al-tolerance cultivar (Koshihikari) and an Al-sensitive mutant star1 (SENSITIVE TO AL RHIZOTOXICITY 1) in both the root tips and the basal roots. Exposure to 20 mu M AlCl3 for 6 h resulted in up-regulation (higher than 3-fold) of 213 and 2015 genes including 185 common genes in the root tips of wild-type and the mutant, respectively. On the other hand, in the basal root, genes up-regulated by Al were 126 and 2419 including 76 common genes in the wild-type and the mutant, respectively. These results indicate that Al-response genes are not only restricted to the root tips, but also in the basal root region. Analysis with genes up-or down-regulated only in the wild-type reveals that there are other mechanisms for Al-tolerance except for a known transcription factor ART1-regulated one in rice. These mechanisms are related to nitrogen assimilation, secondary metabolite synthesis, cell-wall synthesis and ethylene synthesis. Although the exact roles of these putative tolerance genes remain to be examined, our data provide a platform for further work on Al-tolerance in rice.

    DOI: 10.1371/journal.pone.0048197

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  • イネケイ酸輸送体Lsi2(SIET1)のホモログSIET3‐5の解析

    久家徳之, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集58   58   2012年9月

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  • P10-12 OsNramp3によるマンガン分配制御機構(10.植物の微量栄養素,2012年度鳥取大会)

    佐々木 明正, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集 ( 58 )   2012年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • YSL16 Is a Phloem-Localized Transporter of the Copper-Nicotianamine Complex That Is Responsible for Copper Distribution in Rice

    Luqing Zheng, Naoki Yamaji, Kengo Yokosho, Jian Feng Ma

    PLANT CELL24 ( 9 ) 3767 - 3782   2012年9月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Cu is an essential element for plant growth, but the molecular mechanisms of its distribution and redistribution within the plants are unknown. Here, we report that Yellow stripe-like16 (YSL16) is involved in Cu distribution and redistribution in rice (Oryza sativa). Rice YSL16 was expressed in the roots, leaves, and unelongated nodes at the vegetative growth stage and highly expressed in the upper nodes at the reproductive stage. YSL16 was expressed at the phloem of nodes and vascular tissues of leaves. Knockout of this gene resulted in a higher Cu concentration in the older leaves but a lower concentration in the younger leaves at the vegetative stage. At the reproductive stage, a higher Cu concentration was found in the flag leaf and husk, but less Cu was present in the brown rice, resulting in a significant reduction in fertility in the knockout line. Isotope labeling experiments with Cu-65 showed that the mutant lost the ability to transport Cu-nicotianamine from older to younger leaves and from the flag leaf to the panicle. Rice YSL16 transported the Cu-nicotianamine complex in yeast. Taken together, our results indicate that Os-YSL16 is a Cu-nicotianamine transporter that is required for delivering Cu to the developing young tissues and seeds through phloem transport.

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  • Up-Regulation of a Magnesium Transporter Gene OsMGT1 Is Required for Conferring Aluminum Tolerance in Rice

    Zhi Chang Chen, Naoki Yamaji, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

    PLANT PHYSIOLOGY159 ( 4 ) 1624 - 1633   2012年8月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Magnesium (Mg)-mediated alleviation of aluminum (Al) toxicity has been observed in a number of plant species, but the mechanisms underlying the alleviation are still poorly understood. When a putative rice (Oryza sativa) Mg transporter gene, Oryza sativa MAGNESIUM TRANSPORTER1 (OsMGT1), was knocked out, the tolerance to Al, but not to cadmium and lanthanum, was decreased. However, this inhibition could be rescued by addition of 10 mu M Mg, but not by the same concentration of barium or strontium. OsMGT1 was expressed in both the roots and shoots in the absence of Al, but the expression only in the roots was rapidly up-regulated by Al. Furthermore, the expression did not respond to low pH and other metals including cadmium and lanthanum, and was regulated by an Al-responsive transcription factor, AL RESISTANCE TRANSCRIPTION FACTOR1. An investigation of subcellular localization showed that OsMGT1 was localized to the plasma membrane. A short-term (30 min) uptake experiment with stable isotope Mg-25 showed that knockout of OsMGT1 resulted in decreased Mg uptake, but that the uptake in the wild type was enhanced by Al. Mg concentration in the cell sap of the root tips was also increased in the wild-type rice, but not in the knockout lines in the presence of Al. A microarray analysis showed that transcripts of genes related to stress were more up- and down-regulated in the knockout lines. Taken together, our results indicate that OsMGT1 is a transporter for Mg uptake in the roots and that up-regulation of this gene is required for conferring Al tolerance in rice by increasing Mg concentration in the cell.

    DOI: 10.1104/pp.112.199778

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  • Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation

    Matthew J. Milner, Eric Craft, Naoki Yamaji, Emi Koyama, Jian Feng Ma, Leon V. Kochian

    NEW PHYTOLOGIST195 ( 1 ) 113 - 123   2012年7月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the zinc (Zn)/cadmium (Cd) hyperaccumulating plant species, Noccaea caerulescens, formerly known as Thlaspi caerulescens. NcZNT1 was previously suggested to be the primary root Zn/Cd uptake transporter. Both a characterization of NcZNT1 transport function in planta and in heterologous systems, and an analysis of NcZNT1 gene expression and NcZNT1 protein localization were carried out. We show that NcZNT1 is not only expressed in the root epidermis, but also is highly expressed in the root and shoot vasculature, suggesting a role in long-distance metal transport. Also, NcZNT1 was found to be a plasma membrane transporter that mediates Zn but not Cd, iron (Fe), manganese (Mn) or copper (Cu) uptake into plant cells. Two novel regions of the NcZNT1 promoter were identified which may be involved in both the hyperexpression of NcZNT1 and its ability to be regulated by plant Zn status. In conclusion, we demonstrate here that NcZNT1 plays a role in Zn and not Cd uptake from the soil, and based on its strong expression in the root and shoot vasculature, could be involved in long-distance transport of Zn from the root to the shoot via the xylem.

    DOI: 10.1111/j.1469-8137.2012.04144.x

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  • Transcriptional regulation of aluminium tolerance genes

    Emmanuel Delhaize, Jian Feng Ma, Peter R. Ryan

    TRENDS IN PLANT SCIENCE17 ( 6 ) 341 - 348   2012年6月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:ELSEVIER SCIENCE LONDON  

    Trivalent aluminium (Al3+) is the major toxin encountered by plants on acid soils. These cations inhibit root growth by damaging cells at the root apex. The physiology and genetics of Al3+ tolerance mechanisms involving organic anion efflux from roots have now been investigated in a range of species. Over the past decade, genes encoding these and other newly discovered mechanisms of tolerance have been cloned. In this review, we describe the genes controlling the genotypic variation in Al3+ tolerance for several important crop species. We focus on recent insights into the transcriptional regulation of these and other genes involved in Al3+ tolerance and discuss the pathways coordinating their expression in Arabidopsis and rice.

    DOI: 10.1016/j.tplants.2012.02.008

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  • Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene

    Jonatan Montpetit, Julien Vivancos, Namiki Mitani-Ueno, Naoki Yamaji, Wilfried Remus-Borel, Francois Belzile, Jian Feng Ma, Richard R. Belanger

    PLANT MOLECULAR BIOLOGY79 ( 1-2 ) 35 - 46   2012年5月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Silicon (Si) is known to be beneficial to plants, namely in alleviating biotic and abiotic stresses. The magnitude of such positive effects is associated with a plant's natural ability to absorb Si. Many grasses can accumulate as much as 10% on a dry weight basis while most dicots, including Arabidopsis, will accumulate less than 0.1%. In this report, we describe the cloning and functional characterization of TaLsi1, a wheat Si transporter gene. In addition, we developed a heterologous system for the study of Si uptake in plants by introducing TaLsi1 and OsLsi1, its ortholog in rice, into Arabidopsis, a species with a very low innate Si uptake capacity. When expressed constitutively under the control of the CaMV 35S promoter, both TaLsi1 and OsLsi1 were expressed in cells of roots and shoots. Such constitutive expression of TaLsi1 or OsLsi1 resulted in a fourfold to fivefold increase in Si accumulation in transformed plants compared to WT. However, this Si absorption caused deleterious symptoms. When the wheat transporter was expressed under the control of a root-specific promoter (a boron transporter gene (AtNIP5;1) promoter), a similar increase in Si absorption was noted but the plants did not exhibit symptoms and grew normally. These results demonstrate that TaLsi1 is indeed a functional Si transporter as its expression in Arabidopsis leads to increased Si uptake, but that this expression must be confined to root cells for healthy plant development. The availability of this heterologous expression system will facilitate further studies into the mechanisms and benefits of Si uptake.

    DOI: 10.1007/s11103-012-9892-3

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  • Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene

    Jonatan Montpetit, Julien Vivancos, Namiki Mitani-Ueno, Naoki Yamaji, Wilfried Remus-Borel, Francois Belzile, Jian Feng Ma, Richard R. Belanger

    PLANT MOLECULAR BIOLOGY79 ( 1-2 ) 35 - 46   2012年5月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Silicon (Si) is known to be beneficial to plants, namely in alleviating biotic and abiotic stresses. The magnitude of such positive effects is associated with a plant's natural ability to absorb Si. Many grasses can accumulate as much as 10% on a dry weight basis while most dicots, including Arabidopsis, will accumulate less than 0.1%. In this report, we describe the cloning and functional characterization of TaLsi1, a wheat Si transporter gene. In addition, we developed a heterologous system for the study of Si uptake in plants by introducing TaLsi1 and OsLsi1, its ortholog in rice, into Arabidopsis, a species with a very low innate Si uptake capacity. When expressed constitutively under the control of the CaMV 35S promoter, both TaLsi1 and OsLsi1 were expressed in cells of roots and shoots. Such constitutive expression of TaLsi1 or OsLsi1 resulted in a fourfold to fivefold increase in Si accumulation in transformed plants compared to WT. However, this Si absorption caused deleterious symptoms. When the wheat transporter was expressed under the control of a root-specific promoter (a boron transporter gene (AtNIP5;1) promoter), a similar increase in Si absorption was noted but the plants did not exhibit symptoms and grew normally. These results demonstrate that TaLsi1 is indeed a functional Si transporter as its expression in Arabidopsis leads to increased Si uptake, but that this expression must be confined to root cells for healthy plant development. The availability of this heterologous expression system will facilitate further studies into the mechanisms and benefits of Si uptake.

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  • Nramp5 Is a Major Transporter Responsible for Manganese and Cadmium Uptake in Rice

    Akimasa Sasaki, Naoki Yamaji, Kengo Yokosho, Jian Feng Ma

    PLANT CELL24 ( 5 ) 2155 - 2167   2012年5月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Paddy rice (Oryza sativa) is able to accumulate high concentrations of Mn without showing toxicity; however, the molecular mechanisms underlying Mn uptake are unknown. Here, we report that a member of the Nramp (for the Natural Resistance-Associated Macrophage Protein) family, Nramp5, is involved in Mn uptake and subsequently the accumulation of high concentrations of Mn in rice. Nramp5 was constitutively expressed in the roots and encodes a plasma membrane-localized protein. Nramp5 was polarly localized at the distal side of both exodermis and endodermis cells. Knockout of Nramp5 resulted in a significant reduction in growth and grain yield, especially when grown at low Mn concentrations. This growth reduction could be partially rescued by supplying high concentrations of Mn but not by the addition of Fe. Mineral analysis showed that the concentration of Mn and Cd in both the roots and shoots was lower in the knockout line than in wild-type rice. A short-term uptake experiment revealed that the knockout line lost the ability to take up Mn and Cd. Taken together, Nramp5 is a major transporter of Mn and Cd and is responsible for the transport of Mn and Cd from the external solution to root cells.

    DOI: 10.1105/tpc.112.096925

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  • ソバのアルミニウム誘導性輸送体遺伝子(FeIREG2)の機能解析

    横正健剛, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集53rd   303   2012年3月

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  • Acquisition of aluminium tolerance by modification of a single gene in barley. 国際誌

    Miho Fujii, Kengo Yokosho, Naoki Yamaji, Daisuke Saisho, Miki Yamane, Hirokazu Takahashi, Kazuhiro Sato, Mikio Nakazono, Jian Feng Ma

    Nature communications3   713 - 713   2012年3月

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    記述言語:英語  

    Originating from the Fertile Crescent in the Middle East, barley has now been cultivated widely on different soil types including acid soils, where aluminium toxicity is a major limiting factor. Here we show that the adaptation of barley to acid soils is achieved by the modification of a single gene (HvAACT1) encoding a citrate transporter. We find that the primary function of this protein is to release citrate from the root pericycle cells to the xylem to facilitate the translocation of iron from roots to shoots. However, a 1-kb insertion in the upstream of the HvAACT1 coding region occurring only in the Al-tolerant accessions, enhances its expression and alters the location of expression to the root tips. The altered HvAACT1 has an important role in detoxifying aluminium by secreting citrate to the rhizosphere. Thus, the insertion of a 1-kb sequence in the HvAACT1 upstream enables barley to adapt to acidic soils.

    DOI: 10.1038/ncomms1726

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  • A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice

    Chao-Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma

    PLANT JOURNAL69 ( 5 ) 857 - 867   2012年3月

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    記述言語:英語   出版者・発行元:WILEY  

    Toxic aluminum enters the root cells rapidly, therefore internal detoxification is required. However, the molecular mechanisms underlying this process are poorly understood. Here we functionally characterized a rice gene, Os03g0755100 (OsALS1), that is regulated by ART1, a C2H2-type zinc finger transcription factor. OsALS1 encodes a half-size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub-group. Expression of OsALS1 was rapidly and specifically induced by Al in the roots, but not by other metals or low pH. OsALS1 was localized at all cells of the roots. Furthermore, OsALS1 is localized to the tonoplast. These expression patterns and cell specificity of localization are different from those of the homologous gene AtALS1 in Arabidopsis. Knockout of OsALS1 in three independent lines resulted in significant increased sensitivity to Al, but did not affect the sensitivity to other metals and low pH. Comparison of Al accumulation patterns between wild-type and osals1 mutants showed that there was no difference in Al levels in the cell sap of root tips between wild-type and the mutants, but the mutants accumulated more Al in the cytosol and nucleus than the wild-type. Expression of OsALS1 in yeast resulted in increased Al sensitivity due to mis-localization. These results indicate that OsALS1 localized at the tonoplast is responsible for sequestration of Al into the vacuoles, which is required for internal detoxification of Al in rice.

    DOI: 10.1111/j.1365-313X.2011.04837.x

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  • A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice

    Chao-Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma

    PLANT JOURNAL69 ( 5 ) 857 - 867   2012年3月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Toxic aluminum enters the root cells rapidly, therefore internal detoxification is required. However, the molecular mechanisms underlying this process are poorly understood. Here we functionally characterized a rice gene, Os03g0755100 (OsALS1), that is regulated by ART1, a C2H2-type zinc finger transcription factor. OsALS1 encodes a half-size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub-group. Expression of OsALS1 was rapidly and specifically induced by Al in the roots, but not by other metals or low pH. OsALS1 was localized at all cells of the roots. Furthermore, OsALS1 is localized to the tonoplast. These expression patterns and cell specificity of localization are different from those of the homologous gene AtALS1 in Arabidopsis. Knockout of OsALS1 in three independent lines resulted in significant increased sensitivity to Al, but did not affect the sensitivity to other metals and low pH. Comparison of Al accumulation patterns between wild-type and osals1 mutants showed that there was no difference in Al levels in the cell sap of root tips between wild-type and the mutants, but the mutants accumulated more Al in the cytosol and nucleus than the wild-type. Expression of OsALS1 in yeast resulted in increased Al sensitivity due to mis-localization. These results indicate that OsALS1 localized at the tonoplast is responsible for sequestration of Al into the vacuoles, which is required for internal detoxification of Al in rice.

    DOI: 10.1111/j.1365-313X.2011.04837.x

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  • Acquisition of aluminium tolerance by modification of a single gene in barley

    Miho Fujii, Kengo Yokosho, Naoki Yamaji, Daisuke Saisho, Miki Yamane, Hirokazu Takahashi, Kazuhiro Sato, Mikio Nakazono, Jian Feng Ma

    NATURE COMMUNICATIONS3   713   2012年3月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Originating from the Fertile Crescent in the Middle East, barley has now been cultivated widely on different soil types including acid soils, where aluminium toxicity is a major limiting factor. Here we show that the adaptation of barley to acid soils is achieved by the modification of a single gene (HvAACT1) encoding a citrate transporter. We find that the primary function of this protein is to release citrate from the root pericycle cells to the xylem to facilitate the translocation of iron from roots to shoots. However, a 1-kb insertion in the upstream of the HvAACT1 coding region occurring only in the Al-tolerant accessions, enhances its expression and alters the location of expression to the root tips. The altered HvAACT1 has an important role in detoxifying aluminium by secreting citrate to the rhizosphere. Thus, the insertion of a 1-kb sequence in the HvAACT1 upstream enables barley to adapt to acidic soils.

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  • A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots

    Chao-Feng Huang, Naoki Yamaji, Kazuko Ono, Jian Feng Ma

    PLANT JOURNAL69 ( 4 ) 565 - 576   2012年2月

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    記述言語:英語   出版者・発行元:WILEY  

    Root outer cell layers of Oryza sativa (rice), which comprise the epidermis, exodermis and sclerenchyma, play an important role in protecting the roots from various stresses in soil, but the molecular mechanisms for the specification of these cell layers are poorly understood. In this work, we report on defective in outer cell layer specification 1 (Docs1), which is involved in the specification of outer cell layers in rice roots. Docs1 was isolated by map-based cloning using a mutant (c68) defective in the outer cell layers of primary roots. It encodes a leucine-rich repeat receptor-like kinase (LRR RLK). Docs1 mRNA was expressed in all tissues including roots, leaf blades and sheaths, and flowers. Immunostaining with an anti-Docs1 antibody showed that Docs1 was localized at the epidermis and exodermis, depending on the root region. Furthermore, Docs1 showed polar localization at the distal side. Subcellular examination showed that Docs1 was localized to the plasma membrane. Comparison of genome-wide transcriptional profiles between the wild-type and the knock-out mutant roots using microarray analysis showed that 61 and 41 genes were up- and downregulated in the mutant, including genes encoding putative transcription factors and genes potentially involved in cell wall metabolism. These results suggest that Docs1 might directly or indirectly regulate multiple genes involved in the proper development of root outer cell layers in rice.

    DOI: 10.1111/j.1365-313X.2011.04824.x

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  • A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots

    Chao-Feng Huang, Naoki Yamaji, Kazuko Ono, Jian Feng Ma

    Plant Journal69 ( 4 ) 565 - 576   2012年2月

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    記述言語:英語  

    Root outer cell layers of Oryza sativa (rice), which comprise the epidermis, exodermis and sclerenchyma, play an important role in protecting the roots from various stresses in soil, but the molecular mechanisms for the specification of these cell layers are poorly understood. In this work, we report on defective in outer cell layer specification 1 (Docs1), which is involved in the specification of outer cell layers in rice roots. Docs1 was isolated by map-based cloning using a mutant (c68) defective in the outer cell layers of primary roots. It encodes a leucine-rich repeat receptor-like kinase (LRR RLK). Docs1 mRNA was expressed in all tissues including roots, leaf blades and sheaths, and flowers. Immunostaining with an anti-Docs1 antibody showed that Docs1 was localized at the epidermis and exodermis, depending on the root region. Furthermore, Docs1 showed polar localization at the distal side. Subcellular examination showed that Docs1 was localized to the plasma membrane. Comparison of genome-wide transcriptional profiles between the wild-type and the knock-out mutant roots using microarray analysis showed that 61 and 41 genes were up-and downregulated in the mutant, including genes encoding putative transcription factors and genes potentially involved in cell wall metabolism. These results suggest that Docs1 might directly or indirectly regulate multiple genes involved in the proper development of root outer cell layers in rice. © 2011 Blackwell Publishing Ltd.

    DOI: 10.1111/j.1365-313X.2011.04824.x

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  • 近年の農業へのケイ酸利用と研究.1.植物のケイ酸栄養と輸送.

    山地直樹, 馬 建鋒

    日本土壌肥料学雑誌83   319 - 325   2012年

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  • Silicon deficiency promotes lignin accumulation in rice

    Shiro Suzuki, Jian Feng Ma, Naoki Yamamoto, Takefumi Hattori, Masahiro Sakamoto, Toshiaki Umezawa

    PLANT BIOTECHNOLOGY29 ( 4 ) 391 - 394   2012年

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    記述言語:英語   出版者・発行元:JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY  

    Although both lignin and silica accumulate on cell walls and confer rigidity, mechanical strength, and resistance to pathogen invasion in rice, it remains unclear whether silicon deficiency affects lignin accumulation. We demonstrate that low silicon rice mutated in a silicon influx transporter, Lsi1, or a silicon efflux transporter, Lsi2, contained larger amounts of lignin in the rice straws. Furthermore, wild-type rice cultivated on low silicon media also accumulated larger amounts of lignin in shoots. Significant accumulation of guaiacyl lignin upon silicon deficiency was determined by nitrobenzene oxidation analysis. These data indicate a negative correlation between silicon accumulation and lignin deposition.

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  • A MATE-type transporter responsible for iron supply to nodule infection zone of Lotus japonicus.

    高梨功次郎, 横正健剛, 高橋宏和, 佐伯和彦, 杉山暁史, 佐藤修正, 田畑哲之, 中園幹生, 馬建鋒, 矢崎一史

    第15回国際分子・植物・微生物相互作用学会(IS-MPMI) 2012.7.29-2012.8.2   2012年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)  

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  • 11-9 イネのMATE遺伝子OsFRDL2の更なる機能解析(11.植物の有害元素,2012年度鳥取大会)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集58 ( 0 )   2012年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 11-11 オオムギのアルミニウム耐性機構の獲得(11.植物の有害元素,2012年度鳥取大会)

    柏野 美帆, 横正 健剛, 山地 直樹, 最相 大輔, 山根 美樹, 高橋 宏和, 佐藤 和広, 中園 幹生, 馬 建鋒

    日本土壌肥料学会講演要旨集58 ( 0 )   2012年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P9-3 ケイ酸輸送体Lsi1のケイ酸応答性および組織局在性を制御するプロモーターの解析(9.植物の多量栄養素,2012年度鳥取大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集58 ( 0 )   2012年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • ケイ素欠乏はイネ地上部におけるリグニンの蓄積を促進する

    鈴木史朗, 馬建鋒, 山本直樹, 服部武文, 坂本正弘, 梅澤俊明

    第30回日本植物細胞分子生物学会(生駒)大会・シンポジウム講演要旨集,72   2012年

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  • OsYSL6 Is Involved in the Detoxification of Excess Manganese in Rice

    Akimasa Sasaki, Naoki Yamaji, Jixing Xia, Jian Feng Ma

    PLANT PHYSIOLOGY157 ( 4 ) 1832 - 1840   2011年12月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Yellow Stripe-Like (YSL) proteins belong to the oligopeptide transporter family and have been implicated in metal transport and homeostasis in different plant species. Here, we functionally characterized a rice (Oryza sativa) YSL member, OsYSL6. Knockout of OsYSL6 resulted in decreased growth of both roots and shoots only in the high-manganese (Mn) condition. There was no difference in the concentration of total Mn and other essential metals between the wild-type rice and the knockout line, but the knockout line showed a higher Mn concentration in the leaf apoplastic solution and a lower Mn concentration in the symplastic solution than wild-type rice. OsYSL6 was constitutively expressed in both the shoots and roots, and the expression level was not affected by either deficiency or toxicity of various metals. Furthermore, the expression level increased with leaf age. Analysis with OsYSL6 promoter-green fluorescent protein transgenic rice revealed that OsYSL6 was expressed in all cells of both the roots and shoots. Heterogolous expression of OsYSL6 in yeast showed transport activity for the Mn-nicotianamine complex but not for the Mn-mugineic acid complex. Taken together, our results suggest that OsYSL6 is a Mn-nicotianamine transporter that is required for the detoxification of excess Mn in rice.

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  • An Al-inducible MATE gene is involved in external detoxification of Al in rice

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    PLANT JOURNAL68 ( 6 ) 1061 - 1069   2011年12月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    A number of plant species, including rice, secretes citrate from roots in response to Al stress. Here we characterized the functions of a gene, OsFRDL4 (Os01g0919100) that belongs to the multidrug and toxic compound extrusion (MATE) family in rice (Oryza sativa). Heterologous expression in Xenopus oocyte showed that the OsFRDL4 protein was able to transport citrate and was activated by Al. The expression level of the OsFRDL4 gene in roots was very low in the absence of Al, but was greatly enhanced by Al after short exposure. Furthermore, the OsFRDL4 expression was regulated by ART1, a C2H2-type zinc finger transcription factor for Al tolerance. Transient expression of OsFRDL4 in onion epidermal cells showed that it localized to the plasma membrane. Immunostaining showed that OsFRDL4 was localized in all cells in the root tip. These expression patterns and cell specificity of localization of OsFRDL4 are different from other MATE members identified previously. Knockout of OsFRDL4 resulted in decreased Al tolerance and decreased citrate secretion compared with the wild-type rice, but did not affect citrate concentration in the xylem sap. Furthermore, there is a positive correlation between OsFRDL4 expression level and the amount of citrate secretion in rice cultivars that are differing in Al tolerance. Taken together, our results show that OsFRDL4 is an Al-induced citrate transporter localized at the plasma membrane of rice root cells and is one of the components of high Al tolerance in rice.

    DOI: 10.1111/j.1365-313X.2011.04757.x

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  • Brachypodium distachyon as a new model system for understanding iron homeostasis in grasses: phylogenetic and expression analysis of Yellow Stripe-Like (YSL) transporters

    Burcu K. Yordem, Sarah S. Conte, Jian Feng Ma, Kengo Yokosho, Kenneth A. Vasques, Srinivasa N. Gopalsamy, Elsbeth L. Walker

    ANNALS OF BOTANY108 ( 5 ) 821 - 833   2011年10月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Background and Aims Brachypodium distachyon is a temperate grass with a small stature, rapid life cycle and completely sequenced genome that has great promise as a model system to study grass-specific traits for crop improvement. Under iron (Fe)-deficient conditions, grasses synthesize and secrete Fe(III)-chelating agents called phytosiderophores (PS). In Zea mays, Yellow Stripe1 (ZmYS1) is the transporter responsible for the uptake of Fe(III)-PS complexes from the soil. Some members of the family of related proteins called Yellow Stripe-Like (YSL) have roles in internal Fe translocation of plants, while the function of other members remains uninvestigated. The aim of this study is to establish brachypodium as a model system to study Fe homeostasis in grasses, identify YSL proteins in brachypodium and maize, and analyse their expression profiles in brachypodium in response to Fe deficiency.Methods The YSL family of proteins in brachypodium and maize were identified based on sequence similarity to ZmYS1. Expression patterns of the brachypodium YSL genes (BdYSL genes) were determined by quantitative RT-PCR under Fe-deficient and Fe-sufficient conditions. The types of PS secreted, and secretion pattern of PS in brachypodium were analysed by high-performance liquid chromatography.Key Results Eighteen YSL family members in maize and 19 members in brachypodium were identified. Phylogenetic analysis revealed that some YSLs group into a grass-specific clade. The Fe status of the plant can regulate expression of brachypodium YSL genes in both shoots and roots. 3-Hydroxy-2'-deoxymugineic acid (HDMA) is the dominant type of PS secreted by brachypodium, and its secretion is diurnally regulated.Conclusions PS secretion by brachypodium parallels that of related crop species such as barley and wheat. A single grass species-specific YSL clade is present, and expression of the BdYSL members of this clade could not be detected in shoots or roots, suggesting grass-specific functions in reproductive tissues. Finally, the Fe-responsive expression profiles of several YSLs suggest roles in Fe homeostasis.

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  • A functional cutin matrix is required for plant protection against water loss

    Guoxiong Chen, Takao Komatsuda, Jian Feng Ma, Chao Li, Naoki Yamaji, Eviatar Nevo

    Plant Signaling and Behavior6 ( 9 ) 1297 - 1299   2011年9月

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    記述言語:英語  

    The plant cuticle, a cutin matrix embedded with and covered by wax, seals the aerial organ's surface to protect the plant against uncontrolled water loss. The cutin matrix is essential for the cuticle to function as a barrier to water loss. Recently, we identified from wild barley a drought supersensitive mutant, eibi1, which is caused by a defective cutin matrix as the result of the loss of function of HvABCG31, an ABCG full transporter. Here, we report that eibi1 epidermal cells contain lipid-like droplets, which are supposed to consist of cutin monomers that have not been transported out of the cells. The eibi1 cuticle is fragile due to a defective cutin matrix. The rice ortholog of the EIBI1 gene has a similar pattern of expression, young shoot but not flag leaf blade, as the barley gene. The model of the function of Eibi1 is discussed. The HvABCG31 full transporter functions in the export of cutin components and contributed to land plant colonization, hence also to terrestrial life evolution. © 2011 Landes Bioscience.

    DOI: 10.4161/psb.6.9.17507

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  • The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic

    Namiki Mitani-Ueno, Naoki Yamaji, Fang-Jie Zhao, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY62 ( 12 ) 4391 - 4398   2011年8月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Nodulin-26-like intrinsic proteins (NIPs) of the aquaporin family are involved in the transport of diverse solutes, but the mechanisms controlling the selectivity of transport substrates are poorly understood. The purpose of this study was to investigate how the aromatic/arginine (ar/R) selectivity filter influences the substrate selectivity of two NIP aquaporins; the silicic acid (Si) transporter OsLsi1 (OsNIP2;1) from rice and the boric acid (B) transporter AtNIP5;1 from Arabidopsis; both proteins are also permeable to arsenite. Native and site-directed mutagenized variants of the two genes were expressed in Xenopus oocytes and the transport activities for Si, B, arsenite, and water were assayed. Substitution of the amino acid at the ar/R second helix (H2) position of OsLsi1 did not affect the transport activities for Si, B, and arsenite, but that at the H5 position resulted in a total loss of Si and B transport activities and a partial loss of arsenite transport activity. Conversely, changes of the AtNIP5;1 ar/R selectivity filter and the NPA motifs to the OsLsi1 type did not result in a gain of Si transport activity. B transport activity was partially lost in the H5 mutant but unaffected in the H2 mutant of AtNIP5;1. In contrast, both the single and double mutations at the H2 and/or H5 positions of AtNIP5;1 did not affect arsenite transport activity. The results reveal that the residue at the H5 position of the ar/R filter of both OsLsi1 and AtNIP5;1 plays a key role in the permeability to Si and B, but there is a relatively low selectivity for arsenite.

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  • Transport of silicon from roots to panicles in plants

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani-Ueno

    PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES87 ( 7 ) 377 - 385   2011年7月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:JAPAN ACAD  

    Silicon (Si) is the most abundant minerals in soil and exerts beneficial effects on plant growth by alleviating various stresses. The transport of Si from soil to the panicles is mediated by different transporters. Lsi1, belonging to a NIP group of the aquaporin family, is responsible for the uptake of Si from soil into the root cells in both dicots and monocots although its expression patterns and cellular localization differ with plant species. The subsequent transport of Si out of the root cells towards the stele is medicated by an active efflux transporter, Lsi2. Lsi1. and Lsi2 are polarly localized at the distal and proximal sides, respectively, of both exodermis and endodermis in rice root. Silicon in the xylem sap is presented in the form of monosilicic acid and is unloaded by Lsi6, a homolog of Lsi1 in rice. Lsi6 is also involved in the inter-vascular transfer of Si at the node, which is necessary for preferential Si distribution to the panicles.

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  • An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice

    Guoxiong Chen, Takao Komatsuda, Jian Feng Ma, Christiane Nawrath, Mohammad Pourkheirandish, Akemi Tagiri, Yin-Gang Hu, Mohammad Sameri, Xinrong Li, Xin Zhao, Yubing Liu, Chao Li, Xiaoying Ma, Aidong Wang, Sudha Nair, Ning Wang, Akio Miyao, Shun Sakuma, Naoki Yamaji, Xiuting Zheng, Eviatar Nevo

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA108 ( 30 ) 12354 - 12359   2011年7月

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    記述言語:英語   出版者・発行元:NATL ACAD SCIENCES  

    Land plants have developed a cuticle preventing uncontrolled water loss. Here we report that an ATP-binding cassette (ABC) subfamily G (ABCG) full transporter is required for leaf water conservation in both wild barley and rice. A spontaneous mutation, eibi1.b, in wild barley has a low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. Map-based cloning revealed that Eibi1 encodes an HvABCG31 full transporter. The gene was highly expressed in the elongation zone of a growing leaf (the site of cutin synthesis), and its gene product also was localized in developing, but not in mature tissue. A de novo wild barley mutant named "eibi1.c," along with two transposon insertion lines of rice mutated in the ortholog of HvABCG31 also were unable to restrict water loss from detached leaves. HvABCG31 is hypothesized to function as a transporter involved in cutin formation. Homologs of HvABCG31 were found in green algae, moss, and lycopods, indicating that this full transporter is highly conserved in the evolution of land plants.

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  • An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice

    Guoxiong Chen, Takao Komatsuda, Jian Feng Ma, Christiane Nawrath, Mohammad Pourkheirandish, Akemi Tagiri, Yin-Gang Hu, Mohammad Sameri, Xinrong Li, Xin Zhao, Yubing Liu, Chao Li, Xiaoying Ma, Aidong Wang, Sudha Nair, Ning Wang, Akio Miyao, Shun Sakuma, Naoki Yamaji, Xiuting Zheng, Eviatar Nevo

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA108 ( 30 ) 12354 - 12359   2011年7月

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    記述言語:英語   出版者・発行元:NATL ACAD SCIENCES  

    Land plants have developed a cuticle preventing uncontrolled water loss. Here we report that an ATP-binding cassette (ABC) subfamily G (ABCG) full transporter is required for leaf water conservation in both wild barley and rice. A spontaneous mutation, eibi1.b, in wild barley has a low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. Map-based cloning revealed that Eibi1 encodes an HvABCG31 full transporter. The gene was highly expressed in the elongation zone of a growing leaf (the site of cutin synthesis), and its gene product also was localized in developing, but not in mature tissue. A de novo wild barley mutant named "eibi1.c," along with two transposon insertion lines of rice mutated in the ortholog of HvABCG31 also were unable to restrict water loss from detached leaves. HvABCG31 is hypothesized to function as a transporter involved in cutin formation. Homologs of HvABCG31 were found in green algae, moss, and lycopods, indicating that this full transporter is highly conserved in the evolution of land plants.

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  • Elevated expression of TcHMA3 plays a key role in the extreme Cd tolerance in a Cd-hyperaccumulating ecotype of Thlaspi caerulescens

    Daisei Ueno, Matthew J. Milner, Naoki Yamaji, Kengo Yokosho, Emi Koyama, M. Clemencia Zambrano, Molly Kaskie, Stephen Ebbs, Leon V. Kochian, Jian Feng Ma

    PLANT JOURNAL66 ( 5 ) 852 - 862   2011年6月

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    記述言語:英語   出版者・発行元:WILEY  

    P>Cadmium (Cd) is a highly toxic heavy metal for plants, but several unique Cd-hyperaccumulating plant species are able to accumulate this metal to extraordinary concentrations in the aboveground tissues without showing any toxic symptoms. However, the molecular mechanisms underlying this hypertolerance to Cd are poorly understood. Here we have isolated and functionally characterized an allelic gene, TcHMA3 (heavy metal ATPase 3) from two ecotypes (Ganges and Prayon) of Thlaspi caerulescens contrasting in Cd accumulation and tolerance. The TcHMA3 alleles from the higher (Ganges) and lower Cd-accumulating ecotype (Prayon) share 97.8% identity, and encode a P-1B-type ATPase. There were no differences in the expression pattern, cell-specificity of protein localization and transport substrate-specificity of TcHMA3 between the two ecotypes. Both alleles were characterized by constitutive expression in the shoot and root, a tonoplast localization of the protein in all leaf cells and specific transport activity for Cd. The only difference between the two ecotypes was the expression level of TcHMA3: Ganges showed a sevenfold higher expression than Prayon, partly caused by a higher copy number. Furthermore, the expression level and localization of TcHMA3 were different from AtHMA3 expression in Arabidopsis. Overexpression of TcHMA3 in Arabidopsis significantly enhanced tolerance to Cd and slightly increased tolerance to Zn, but did not change Co or Pb tolerance. These results indicate that TcHMA3 is a tonoplast-localized transporter highly specific for Cd, which is responsible for sequestration of Cd into the leaf vacuoles, and that a higher expression of this gene is required for Cd hypertolerance in the Cd-hyperaccumulating ecotype of T. caerulescens.

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  • High-Resolution Secondary Ion Mass Spectrometry Reveals the Contrasting Subcellular Distribution of Arsenic and Silicon in Rice Roots

    Katie L. Moore, Markus Schroeder, Zhongchang Wu, Barry G. H. Martin, Chris R. Hawes, Steve P. McGrath, Malcolm J. Hawkesford, Jian Feng Ma, Fang-Jie Zhao, Chris R. M. Grovenor

    PLANT PHYSIOLOGY156 ( 2 ) 913 - 924   2011年6月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) takes up arsenite mainly through the silicic acid transport pathway. Understanding the uptake and sequestration of arsenic (As) into the rice plant is important for developing strategies to reduce As concentration in rice grain. In this study, the cellular and subcellular distributions of As and silicon (Si) in rice roots were investigated using high-pressure freezing, high-resolution secondary ion mass spectrometry, and transmission electron microscopy. Rice plants, both the lsi2 mutant lacking the Si/arsenite efflux transporter Lsi2 and its wild-type cultivar, with or without an iron plaque, were treated with arsenate or arsenite. The formation of iron plaque on the root surface resulted in strong accumulation of As and phosphorous on the epidermis. The lsi2 mutant showed stronger As accumulation in the endodermal vacuoles, where the Lsi2 transporter is located in the plasma membranes, than the wild-type line. As also accumulated in the vacuoles of some xylem parenchyma cells and in some pericycle cells, particularly in the wild-type mature root zone. Vacuolar accumulation of As is associated with sulfur, suggesting that As may be stored as arsenite-phytochelatin complexes. Si was localized in the cell walls of the endodermal cells with little apparent effect of the Lsi2 mutation on its distribution. This study reveals the vacuolar sequestration of As in rice roots and contrasting patterns of As and Si subcellular localization, despite both being transported across the plasma membranes by the same transporters.

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  • Identification of a Cis-Acting Element of ART1, a C2H2-Type Zinc-Finger Transcription Factor for Aluminum Tolerance in Rice

    Tomokazu Tsutsui, Naoki Yamaji, Jian Feng Ma

    PLANT PHYSIOLOGY156 ( 2 ) 925 - 931   2011年6月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) is one of the most aluminum (Al)-tolerant species among small-grain cereals. Recent identification of a transcription factor AL RESISTANCE TRANSCRIPTION FACTOR1 (ART1) revealed that this high Al tolerance in rice is achieved by multiple genes involved in detoxification of Al at different cellular levels. ART1 is a C2H2-type zinc-finger transcription factor and regulates the expression of 31 genes in the downstream. In this study, we attempted to identify a cis-acting element of ART1. We used the promoter region of SENSITIVE TO AL RHIZOTOXICITY1, an Al tolerance gene in the downstream of ART1. With the help of gel-shift assay, we were able to identify the cis-acting element as GGN(T/g/a/C)V(C/A/g)S(C/G). This element was found in the promoter region of 29 genes among 31 ART1-regulated genes. To confirm this cis-acting element in vivo, we transiently introduced this element one or five times tandemly repeated sequence with 35S minimal promoter and green fluorescent protein reporter together with or without ART1 gene in the tobacco (Nicotiana tabacum) mesophyll protoplasts. The results showed that the expression of green fluorescent protein reporter responded to ART1 expression. Furthermore, the expression increased with repetition of the cis-acting element. Our results indicate that the five nucleotides identified are the target DNA-binding sequence of ART1.

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  • Isolation and Characterization of a Barley Yellow Stripe-Like Gene, HvYSL5

    Luqing Zheng, Miho Fujii, Naoki Yamaji, Akimasa Sasaki, Miki Yamane, Isamu Sakurai, Kazuhiro Sato, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY52 ( 5 ) 765 - 774   2011年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Yellow stripe-like (YSL) family transporters, belonging to a novel subfamily of oligopeptide transporter (OPT), has been proposed to be involved in metal uptake and long-distance transport, but only a few of them have been functionally characterized so far. In the present study, we isolated an uncharacterized member of the YSL family, HvYSL5, in barley based on expressed sequence tag (EST) information. HvYSL5 shared 50% identity with HvYS1, a transporter for the ferric-mugineic acid complex, at the amino acid level. Promoter analysis showed that the HvYSL5 upstream sequence contains both iron deficiency response element 1 and 2 (IDE1 and 2). HvYSL5 was expressed in the roots and the expression was greatly induced by Fe deficiency, but not by deficiency of other metals including Zn, Cu and Mn. Spatial investigation showed that much higher expression of HvYSL5 was found in the mature zones of the roots, but not in the root tips. Furthermore, the expression showed a diurnal rhythm, being the highest in the morning, but with no expression in the afternoon. HvYSL5 was localized in all root cells, and subcellular localization analysis showed that HvYSL5 is likely to be localized in the vesicles. Knockdown of HvYSL5 did not result in any detectable phenotype changes. Although the exact role of HvYSL5 remains to be examined, our results suggest that it is involved in the transient storage of Fe or phytosiderophores.

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  • Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation

    Namiki Mitani, Naoki Yamaji, Yukiko Ago, Kozo Iwasaki, Jian Feng Ma

    PLANT JOURNAL66 ( 2 ) 231 - 240   2011年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    P>A high accumulation of silicon (Si) is required for overcoming abiotic and biotic stresses, but the molecular mechanisms of Si uptake, especially in dicotyledonous species, is poorly understood. Herein, we report the identification of an influx transporter of Si in two Cucurbita moschata (pumpkin) cultivars greatly differing in Si accumulation, which are used for the rootstocks of bloom and bloomless Cucumis sativus (cucumber), respectively. Heterogeneous expression in both Xenopus oocytes and rice mutant defective in Si uptake showed that the influx transporter from the bloom pumpkin rootstock can transport Si, whereas that from the bloomless rootstock cannot. Analysis with site-directed mutagenesis showed that, among the two amino acid residues differing between the two types of rootstocks, only changing a proline to a leucine at position 242 results in the loss of Si transport activity. Furthermore, all pumpkin cultivars for bloomless rootstocks tested have this mutation. The transporter is localized in all cells of the roots, and investigation of the subcellular localization with different approaches consistently showed that the influx Si transporter from the bloom pumpkin rootstock was localized at the plasma membrane, whereas the one from the bloomless rootstock was localized at the endoplasmic reticulum. Taken together, our results indicate that the difference in Si uptake between two pumpkin cultivars is probably the result of allelic variation in one amino acid residue of the Si influx transporter, which affects the subcellular localization and subsequent transport of Si from the external solution to the root cells.

    DOI: 10.1111/j.1365-313X.2011.04483.x

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  • Further characterization of a rice silicon efflux transporter, Lsi2

    Naoki Yamaji, Jian Feng Ma

    SOIL SCIENCE AND PLANT NUTRITION57 ( 2 ) 259 - 264   2011年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    High silicon (Si) accumulation is required for high and sustainable production of rice. The uptake of Si by rice roots is mediated by both influx transporter Lsi1 and efflux transporter Lsi2. Here we further characterized Lsi2 in terms of localization and expression patterns. Analysis with promoter-GFP transgenic rice revealed that Lsi2 was expressed in the main and lateral roots, but not in the root hairs. In lateral roots, Lsi2 was localized at the proximal side of both exodermis and endodermis like main roots. The expression of Lsi2 gene was down-regulated by Si in the wild-type rice, but unaffected in the lsi2 mutant, suggesting that the expression of Lsi2 is regulated by Si accumulated in the shoots. Treatment with drought and abscisic acid rapidly resulted in decreased Lsi2 expression. Monitoring of Lsi2 expression at different growth stage showed a transient increase around the heading stage in rice grown in a paddy field. These expression patterns are similar to those of Lsi1, indicating that Lsi2 is co-regulated with Lsi1 and the expression of these two genes responds to both environmental condition and plant Si requirement.

    DOI: 10.1080/00380768.2011.565480

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  • Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan

    Daisei Ueno, Emi Koyama, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY62 ( 7 ) 2265 - 2272   2011年4月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Cadmium (Cd) in rice is a major source of Cd intake for people on a staple rice diet. The mechanisms underlying Cd accumulation in rice plant are still poorly understood. Here, we characterized the physiology and genetics of Cd transport in a high-Cd-accumulating cultivar (Jarjan) of rice (Oryza sativa). Jarjan showed 5- to 34-fold higher Cd accumulation in the shoots and grains than the cultivar Nipponbare, when it was grown in either a non-Cd-contaminated or a Cd-contaminated soil. A short-term uptake experiment showed no significant difference in Cd uptake by the roots between the two cultivars. However, Jarjan translocated 49% of the total Cd taken up to the shoots, whereas Nipponbare retained most of the Cd in the roots. In both concentration- and time-dependent experiments, Jarjan showed a superior capacity for root-to-shoot translocation of Cd. These results indicate that the high-Cd-accumulation phenotype in Jarjan results from efficient translocation of Cd from roots to shoots. Genetic analysis using an F(2) population derived from Jarjan and Nipponbare revealed that plants showing high- and low-Cd-accumulation phenotypes segregated in a 1:3 ratio, indicating that high accumulation in Jarjan is controlled by a single recessive gene. Furthermore, we isolated OsHMA3, a gene encoding a tonoplast-localized Cd transporter from Jarjan. The OsHMA3 protein was localized in all roots cells, but the sequence has a mutation leading to loss of function. Therefore, failure to sequester Cd into the root vacuoles by OsHMA3 is probably responsible for high Cd accumulation in Jarjan.

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  • Further characterization of an aluminum influx transporter in rice

    Jixing Xia, Naoki Yamaji, Jian Feng Ma

    Plant Signaling and Behavior6 ( 1 ) 160 - 163   2011年1月

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    記述言語:英語  

    Nrat1 is a plasma membrane-localized aluminum transporter recently identified in rice, which is a member of Nramp family. Here, we further characterized this transporter in terms of transport substrate specificity. Heterologous assay in yeast showed that Al transport activity by Nrat1 was unaffected by the presence of high concentration of Ca, but significantly inhibited by trivalent ions including Yb and Ga, analogs of Al. Knockout of Nrat1 did not affect the uptake of Cd and Mn in rice. On the other hand, over expression of Nrat1 led to enhanced Al uptake by rice roots compared with wild-type rice, but did not affect Cd uptake. These results provide further evidence that unlike other Nramp members, Nrat1 is an influx transporter for trivalent Al ion. © 2011 Landes Bioscience.

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  • 植物の多量栄養素

    牧野 周, 馬 建鋒

    日本土壌肥料学雑誌82 ( 6 ) 495 - 499   2011年

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  • Silicon efflux transporters isolated from two pumpkin cultivars contrasting in Si uptake

    Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    Plant Signaling and Behavior6 ( 7 ) 73 - 76   2011年

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    記述言語:英語  

    The accumulation of silicon (Si) differs greatly with plant species and cultivars due to different ability of the roots to take up Si. In Si accumulating plants such as rice, barley and maize, Si uptake is mediated by the influx (Lsi1) and efflux (Lsi2) transporters. Here we report isolation and functional analysis of two Si efflux transporters (CmLsi2-1 and CmLsi2-2) from two pumpkin (Cucurbita moschata Duch.) cultivars contrasting in Si uptake. These cultivars are used for rootstocks of bloom and bloomless cucumber, respectively. Different from mutations in the Si influx transporter CmLsi1, there was no difference in the sequence of either CmLsi2 between two cultivars. Both CmLsi2-1 and CmLsi2-2 showed an efflux transport activity for Si and they were expressed in both the roots and shoots. These results confirm our previous finding that mutation in CmLsi1, but not in CmLsi2-1 and CmLsi2-2 are responsible for bloomless phenotype resulting from low Si uptake. © 2011 Landes Bioscience.

    DOI: 10.4161/psb.6.7.15462

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  • Further characterization of a rice Si efflux transporter, Lsi2.

    Yamaji, N, Ma, J. F

    Soil Science and Plant Nutrition57   259 - 264   2011年

  • 植物の多量栄養素

    牧野 周, 馬 建鋒

    日本土壌肥料学雑誌82 ( 6 ) 495 - 499   2011年

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  • ミヤコグサ根粒で発現する MATE 型トランスポーターは根粒内の鉄転流に関与している

    高梨功次郎, 高橋宏和, 横正健剛, 杉山暁史, 佐藤修正, 田畑哲之, 馬建鋒, 中園幹生, 矢崎一史

    第21回植物微生物研究交流会 2011.9.20-2011.9.22   2011年

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    記述言語:日本語   掲載種別:研究発表ペーパー・要旨(全国大会,その他学術会議)  

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  • 11-27 ソバのアルミニウム誘導性トランスポーターの単離と機能解析(11.植物の有害元素)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集57 ( 0 )   2011年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 11-30 Identification of the transporter involved in Mn tolerance in rice

    陳 宗慧, 神谷 岳洋, 岩崎 貢三, 加藤 伸一郎, 山地 直樹, 馬 建鋒, 上野 大勢

    日本土壌肥料学会講演要旨集57 ( 0 ) 88 - 88   2011年

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    記述言語:英語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.57.0_88_3

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  • ソバのアルミニウム誘導性輸送体遺伝子の単離と機能解析

    横正 健剛, 山地 直樹, 馬 建鋒

    日本植物生理学会年会およびシンポジウム 講演要旨集2011 ( 0 ) 960 - 960   2011年

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    出版者・発行元:日本植物生理学会  

    ソバはアルミニウム(Al)耐性が強いだけではなく、地上部に高濃度のAlを集積する。生理学的な解析から、ソバはAlに応答し根からシュウ酸を分泌し、地上部ではAlをシュウ酸との錯体で液胞に隔離することが明らかとなっている。これらの過程には多くのトランスポーターの関与が考えられるが未だ同定されていない。私たちはソバのAl耐性及び集積の分子機構を網羅的に解析するために、Alで処理したソバの根からRNAを抽出し次世代シークエンサーを用いてEST解析を行った。均一化したcDNAライブラリーから遺伝子配列の決定を行い約10万のコンティグを作成した。また、均一化していないcDNAライブラリーを用いたショートリード解析から発現量の測定を行った。本研究では、トランスポーター候補遺伝子の中で発現上位にあるFeIREG2についてその全長の単離と機能解析を行った。RACE法を用いて完全長cDNAを単離したところ、FeIREG2はシロイヌナズナのFeとCo、Niの排出トランスポーターAtIREG2とアミノ酸レベルで約60%の相同性を示した。FeIREG2は根で発現し、Al処理6時間後には約20倍に増加していた。この遺伝子とGFPの融合遺伝子をタマネギ表皮細胞に発現させたところ、液胞膜に局在していた。これらのことからFeIREG2はAlの液胞への隔離に関与している可能性が考えられ、更なる解析を進めている。

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  • 11-38 イネ亜ヒ酸排出能の品種間差(11.植物の有害元素)

    大貝 久生, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集57 ( 0 )   2011年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-15 オオムギ低親和性硝酸トランスポーターの機能解析(9.植物の多量栄養素)

    石川 伸二, 園部 和幸, 三谷 奈見季, 山地 直樹, 馬 建鋒, 大山 卓爾, 末吉 邦

    日本土壌肥料学会講演要旨集57 ( 0 )   2011年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-28 ケイ酸輸送体Lsi1の発現制御機構の解析(9.植物の多量栄養素)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集57 ( 0 )   2011年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • トランスポーター研究が・肥料・植物栄養学にもたらすインパクト : 3.アルミニウム耐性に関わるトランスポーター

    馬 建鋒, 山地 直樹

    日本土壌肥料學雜誌81 ( 5 ) 518 - 522   2010年10月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

    DOI: 10.20710/dojo.81.5_518

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  • Plasma membrane-localized transporter for aluminum in rice

    Jixing Xia, Naoki Yamaji, Tomonari Kasai, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA107 ( 43 ) 18381 - 18385   2010年10月

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    記述言語:英語   出版者・発行元:NATL ACAD SCIENCES  

    Aluminum (Al) is the most abundant metal in the Earth's crust, but its trivalent ionic form is highly toxic to all organisms at low concentrations. How Al enters cells has not been elucidated in any organisms. Herein, we report a transporter, Nrat1 (Nramp aluminum transporter 1), specific for trivalent Al ion in rice. Nrat1 belongs to the Nramp (natural resistance-associated macrophage protein) family, but shares a low similarity with other Nramp members. When expressed in yeast, Nrat1 transports trivalent Al ion, but not other divalent ions, such as manganese, iron, and cadmium, or the Al-citrate complex. Nrat1 is localized at the plasma membranes of all cells of root tips except epidermal cells. Knockout of Nrat1 resulted in decreased Al uptake, increased Al binding to cell wall, and enhanced Al sensitivity, but did not affect the tolerance to other metals. Expression of Nrat1 is up-regulated by Al in the roots and regulated by a C2H2 zinc finger transcription factor (ART1). We therefore concluded that Nrat1 is a plasma membrane-localized transporter for trivalent Al, which is required for a prior step of final Al detoxification through sequestration of Al into vacuoles.

    DOI: 10.1073/pnas.1004949107

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  • 11-16 オオムギアルミニウム活性型クエン酸トランスポーター遺伝子HvAACT1の発現調節機構(11.植物の有害元素,2010年度北海道大会)

    藤井 美帆, 山地 直樹, 中園 幹生, 佐藤 和広, 馬 建鋒

    日本土壌肥料学会講演要旨集 ( 56 )   2010年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • Gene limiting cadmium accumulation in rice

    Daisei Ueno, Naoki Yamaji, Izumi Kono, Chao Feng Huang, Tsuyu Ando, Masahiro Yano, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA107 ( 38 ) 16500 - 16505   2010年9月

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    記述言語:英語   出版者・発行元:NATL ACAD SCIENCES  

    Intake of toxic cadmium (Cd) from rice caused Itai-itai disease in the past and it is still a threat for human health. Therefore, control of the accumulation of Cd from soil is an important food-safety issue, but the molecular mechanism for the control is unknown. Herein, we report a gene (OsHMA3) responsible for low Cd accumulation in rice that was isolated from a mapping population derived from a cross between a high and low Cd-accumulating cultivar. The gene encodes a transporter belonging to the P(1B)-type ATPase family, but shares low similarity with other members. Heterologous expression in yeast showed that the transporter from the low-Cd cultivar is functional, but the transporter from the high-Cd cultivar had lost its function, probably because of the single amino acid mutation. The transporter is mainly expressed in the tonoplast of root cells at a similar level in both the low and high Cd-accumulating cultivars. Overexpression of the functional gene from the low Cd-accumulating cultivar selectively decreased accumulation of Cd, but not other micronutrients in the grain. Our results indicated that OsHMA3 from the low Cd-accumulating cultivar limits translocation of Cd from the roots to the above-ground tissues by selectively sequestrating Cd into the root vacuoles.

    DOI: 10.1073/pnas.1005396107

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  • Knockout of a Bacterial-Type ATP-Binding Cassette Transporter Gene, AtSTAR1, Results in Increased Aluminum Sensitivity in Arabidopsis

    Chao-Feng Huang, Naoki Yamaji, Jian Feng Ma

    PLANT PHYSIOLOGY153 ( 4 ) 1669 - 1677   2010年8月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    ATP-binding cassette (ABC) transporters represent a large family in plants, but the functions of most of these transporters are unknown. Here we report a gene, AtSTAR1, only encoding an ATP-binding domain of a bacterial-type ABC transporter in Arabidopsis (Arabidopsis thaliana). AtSTAR1 is an ortholog of rice (Oryza sativa) OsSTAR1, which has been implicated in aluminum (Al) tolerance. Knockout of AtSTAR1 resulted in increased sensitivity to Al and earlier flowering. Unlike OsSTAR1, AtSTAR1 was expressed in both the roots and shoots and its expression was not induced by Al or other stresses. Investigation of tissue-specific localization of AtSTAR1 through beta-glucuronidase fusion revealed that AtSTAR1 was predominantly expressed at outer cell layers of root tips and developing leaves, whose localization is also different from those of OsSTAR1. However, introduction of OsSTAR1 into atstar1 mutant rescued the sensitivity of atstar1 to Al, indicating that AtSTAR1 has a similar function as OsSTAR1. Furthermore, we found that AtSTAR1 may interact with ALS3, a transmembrane-binding domain in Arabidopsis to form a complex because introduction of OsSTAR1, a functional substitute of AtSTAR1, into als3 mutant resulted in the loss of OsSTAR1 protein. All these findings indicate that AtSTAR1 is involved in the basic detoxification of Al in Arabidopsis.

    DOI: 10.1104/pp.110.155028

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  • Involvement of Silicon Influx Transporter OsNIP2;1 in Selenite Uptake in Rice

    Xue Qiang Zhao, Namiki Mitani, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    PLANT PHYSIOLOGY153 ( 4 ) 1871 - 1877   2010年8月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) as a staple food, provides a major source of dietary selenium (Se) for humans, which essentially requires Se, however, the molecular mechanism for Se uptake is still poorly understood. Herein, we show evidence that the uptake of selenite, a main bioavailable form of Se in paddy soils, is mediated by a silicon (Si) influx transporter Lsi1 (OsNIP2;1) in rice. Defect of OsNIP2;1 resulted in a significant decrease in the Se concentration of the shoots and xylem sap when selenite was given. However, there was no difference in the Se concentration between the wild-type rice and mutant of OsNIP2;1 when selenate was supplied. A short-term uptake experiment showed that selenite uptake greatly increased with decreasing pH in the external solution. Si as silicic acid did not inhibit the Se uptake from selenite in both rice and yeast (Saccharomyces cerevisiae) at low pHs. Expression of OsNIP2;1 in yeast enhanced the selenite uptake at pH 3.5 and 5.5 but not at pH 7.5. On the other hand, defect of Si efflux transporter Lsi2 did not affect the uptake of Se either from selenite or selenate. Taken together, our results indicate that Si influx transporter OsNIP2;1 is permeable to selenite.

    DOI: 10.1104/pp.110.157867

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  • Silicon enhances growth independent of silica deposition in a low-silica rice mutant, lsi1

    Mami Isa, Shuqin Bai, Takushi Yokoyama, Jian Feng Ma, Yushi Ishibashi, Takashi Yuasa, Mari Iwaya-Inoue

    PLANT AND SOIL331 ( 1-2 ) 361 - 375   2010年6月

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    記述言語:英語   出版者・発行元:SPRINGER  

    To examine whether silica bodies are essential for silicon-enhanced growth of rice seedlings, we investigated the response of rice, Oryza sativa L., to silicon treatment. Silicic acid treatment markedly enhanced the SPAD (soil plant analytical development) values of leaf blades and the growth and development of leaves and lateral roots in cvs. Hinohikari and Oochikara, and a low-silicon mutant, lsi1. Combination of ethanol-benzene displacement and staining with crystal violet lactone enabled more detailed histochemical analysis to visualize silica bodies in the epidermis under bright-field microscopy. Supply of silicon induced the development of motor cells and silica bodies in epidermal cells in Hinohikari and Oochikara but not or marginal in lsi1. X-ray analytical microscopy detected silicon specifically in the leaf sheath, the outermost part of the stem, and the leaf blade midrib, suggesting that silicon is distributed to tissues involved in maintaining rigidity of the plant to prevent lodging, rather than being passively deposited in growing tissues. Silicon supplied at high dose accumulated in all rice seedlings and enhanced growth and SPAD values with or without silica body formation. Silicon accumulated in the cell wall may play an important physiological role different from that played by the silica deposited in the motor cell and silica bodies.

    DOI: 10.1007/s11104-009-0258-9

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  • 植物のミネラルストレス応答

    牧野周, 山谷知行, 鎌田淳, 落合久美子, 小山博之, 信濃卓郎, MA Jian Feng, 渡部敏裕

    日本土壌肥料学雑誌81 ( 2 ) 181 - 189   2010年4月

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    記述言語:日本語  

    DOI: 10.20710/dojo.81.2_181

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  • 植物におけるミネラル関連トランスポーター

    植物の成長調節45   49 - 57   2010年

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  • The role of the rice aquaporin Lsi1 in arsenite efflux from roots

    Fang-Jie Zhao, Yukiko Ago, Namiki Mitani, Ren-Ying Li, Yu-Hong Su, Naoki Yamaji, Steve P. McGrath, Jian Feng Ma

    NEW PHYTOLOGIST186 ( 2 ) 392 - 399   2010年

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    When supplied with arsenate (As(V)), plant roots extrude a substantial amount of arsenite (As(III)) to the external medium through as yet unidentified pathways. The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots. Whether Lsi1 also mediates arsenite efflux was investigated.Expression of Lsi1 in Xenopus laevis oocytes enhanced arsenite efflux, indicating that Lsi1 facilitates arsenite transport bidirectionally.Arsenite was the predominant arsenic species in arsenate-exposed rice plants. During 24-h exposure to 5 mu M arsenate, rice roots extruded arsenite to the external medium rapidly, accounting for 60-90% of the arsenate uptake. A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots. By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium.We conclude that Lsi1 plays a role in arsenite efflux in rice roots exposed to arsenate. However, this pathway accounts for only 15-20% of the total efflux, suggesting the existence of other efflux transporters.

    DOI: 10.1111/j.1469-8137.2010.03192.x

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  • Isolation and characterisation of two MATE genes in rye

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    FUNCTIONAL PLANT BIOLOGY37 ( 4 ) 296 - 303   2010年

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    記述言語:英語   出版者・発行元:CSIRO PUBLISHING  

    Multidrug and toxic compound extrusion (MATE) proteins are widely present in bacteria, fungi, plants and mammals. Recent studies have showed that a group of plant MATE genes encodes citrate transporter, which are involved in the detoxification of aluminium or translocation of iron from the roots to the shoots. In this study, we isolated two homologous genes (ScFRDL1 and ScFRDL2) from this family in rye (Secale cereale L.). ScFRDL1 shared 94.2% identity with HvAACT1, an Al-activated citrate transporter in barley (Hordeum vulgare L.) and ScFRDL2 shared 80.6% identity with OsFRDL2, a putative Al-responsive protein in rice (Oryza sativa L.). Both genes were mainly expressed in the roots, however, they showed different expression patterns. Expression of ScFRDL1 was unaffected by Al treatment, but up-regulated by Fe-deficiency treatment. In contrast, expression of ScFRDL2 was greatly induced by Al but not by Fe deficiency. The Al-induced up-regulation of ScFRDL2 was found in both the root tips and basal roots. Furthermore, the expression pattern of ScFRDL2 was consistent with citrate secretion pattern. Immunostaining showed that ScFRDL1 was localised at all cells in the root tips and central cylinder and endodermis in the basal root. Taken together, our results suggest that ScFRDL1 was involved in efflux of citrate into the xylem for Fe translocation from the roots to the shoots, while ScFRDL2 was involved in Al-activated citrate secretion in rye.

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  • 9-4 イネケイ酸輸送体Lsi3の解析(9.植物の多量栄養素,2010年度北海道大会)

    山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集56 ( 0 )   2010年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 11-17 オオムギHvAACT1の5-UTRの挿入配列の解析 : イネによる検証(11.植物の有害元素,2010年度北海道大会)

    横正 健剛, 藤井 美帆, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集56 ( 0 )   2010年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-3 イネケイ酸輸送体遺伝子Lsi1を過剰発現したシロイヌナズナの解析(9.植物の多量栄養素,2010年度北海道大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集56 ( 0 )   2010年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • ライムギ由来MATE遺伝子(ScFRDL1とScFRDL2)の単離と機能解析

    横正 健剛, 山地 直樹, 馬 建鋒

    日本植物生理学会年会およびシンポジウム 講演要旨集2010 ( 0 ) 679 - 679   2010年

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    出版者・発行元:日本植物生理学会  

    Multidrug and toxic compound extrusion(MATE)は排出型の輸送体で生物界に広く存在する。近年、いくつかの植物のMATE遺伝子は外向きのクエン酸輸送タンパク質をコードすることが報告されている。&lt;br&gt;ライムギはAl耐性の強い種とされその耐性は根からの有機酸(リンゴ酸、クエン酸)分泌に由来する。しかし分泌に関与する遺伝子は単離されていない。本研究ではライムギから2つのMATE遺伝子(ScFRDL1,ScFRDL2)を単離し機能解析を行った。まず、オオムギやイネのMATE遺伝子をもとにライムギMATE遺伝子の完全長cDNA配列を決定した。単離されたScFRDL1はオオムギのAl活性型クエン酸トランスポーターHvAACT1とアミノ酸配列で94%、ScFRDL2はイネのAl誘導型MATE遺伝子OsFRDL2と80%の相同性を示した。次に、遺伝子の発現解析を行った。ScFRDL1はAlによる発現の誘導は受けず、鉄欠乏に誘導された。また根の基部側では中心柱に局在していた。一方、ScFRDL2はAlにより発現が誘導され、根からのクエン酸分泌量もこの発現と同様に増加していた。しかし、鉄欠乏による影響は受けなかった。これらの結果からScFRDL1はクエン酸を導管に排出し地上部への鉄輸送に、ScFRDL2はAlによる根圏へのクエン酸分泌に関与することを示している。

    DOI: 10.14841/jspp.2010.0.0679.0

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  • Iron for plants and humans

    Jian Feng Ma, Hong-Qing Ling

    PLANT AND SOIL325 ( 1-2 ) 1 - 3   2009年12月

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    記述言語:英語   出版者・発行元:SPRINGER  

    DOI: 10.1007/s11104-009-0203-y

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  • Identification of a Novel Major Quantitative Trait Locus Controlling Distribution of Cd Between Roots and Shoots in Rice

    Daisei Ueno, Emi Koyama, Izumi Kono, Tsuyu Ando, Masahiro Yano, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY50 ( 12 ) 2223 - 2233   2009年12月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Accumulation of Cd in rice grain is a serious concern of food safety since rice as a staple food is a major source of Cd intake in Asian countries. However, the mechanisms controlling Cd accumulation in rice are still poorly understood. Herein, we report both physiological and genetic analysis of two rice cultivars contrasting in Cd accumulation, which were screened from a core collection of rice cultivars. The cultivar Anjana Dhan (Indica) accumulated much higher levels of Cd than Nipponbare (Japonica) in the shoots and grains when grown in both soil and solution culture. A short-term uptake experiment (20 min) showed that Cd uptake by Nipponbare was higher than that by Anjana Dhan. However, the concentration of Cd in the shoot and xylem sap was much higher in Anjana Dhan than in Nipponbare. Of the Cd taken up by the roots, <4% was translocated to the shoots in Nipponbare, compared with 10-25% in Anjana Dhan, indicating a higher root-to-shoot translocation of Cd in the latter. A quantitative trait locus (QTL) analysis for Cd accumulation was performed using an F-2 population derived from Anjana Dhan and Nipponbare. A QTL with large effect for Cd accumulation was detected on the short arm of chromosome 7, explaining 85.6 of the phenotypic variance in the shoot Cd concentration of the F-2 population. High accumulation is likely to be controlled by a single recessive gene. A candidate genomic region was defined to <1.9 Mb by means of substitution mapping.

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  • A Zinc Finger Transcription Factor ART1 Regulates Multiple Genes Implicated in Aluminum Tolerance in Rice

    Naoki Yamaji, Chao Feng Huang, Sakiko Nagao, Masahiro Yano, Yutaka Sato, Yoshiaki Nagamura, Jian Feng Ma

    PLANT CELL21 ( 10 ) 3339 - 3349   2009年10月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Aluminum (Al) toxicity is the major limiting factor of crop production on acid soils, but some plant species have evolved ways of detoxifying Al. Here, we report a C2H2-type zinc finger transcription factor ART1 (for Al resistance transcription factor 1), which specifically regulates the expression of genes related to Al tolerance in rice (Oryza sativa). ART1 is constitutively expressed in the root, and the expression level is not affected by Al treatment. ART1 is localized in the nucleus of all root cells. A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1, an important factor in rice Al tolerance. Microarray analysis revealed 31 downstream transcripts regulated by ART1, including STAR1 and 2 and a couple of homologs of Al tolerance genes in other plants. Some of these genes were implicated in both internal and external detoxification of Al at different cellular levels. Our findings shed light on comprehensively understanding how plants detoxify aluminum to survive in an acidic environment.

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  • Secretion time of phytosiderophore differs in two perennial grasses and is controlled by temperature

    Daisei Ueno, Jian Feng Ma

    PLANT AND SOIL323 ( 1-2 ) 335 - 341   2009年10月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Some perennial grasses secrete phytosiderophores from the roots in response to Fe-deficiency. Here, we characterized the pattern of phytosiderophore secretion by Lolium perenne (cv. 'Tove') and Poa pratensis (cv. 'Baron'), which are used to correct iron-deficiency induced chlorosis in fruit trees grown on calcareous soils. Both species showed a distinct diurnal rhythm in phytosiderophore secretion, but the secretion time differed between species; the secretion peak time was about 2 h earlier in L. perenne than in P. pratensis under the same growth conditions. The secretion time was shifted by changing temperature during the collection of phytosiderophores in both L. perenne and P. pratensis. Increasing root-zone temperature resulted in earlier secretion, while lowering the temperature resulted in delayed secretion. Furthermore, this shift of secretion time was achieved by changing the temperature around the root-zone. Shading treatment during the secretion period did not affect the secretion time in either species. These results indicate that the secretion of phytosiderophore is triggered by the temperature around the roots, but not light, in these two perennial grasses.

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  • 植物のケイ酸吸収能力の差が新鮮火山灰のアルミニウム富化に及ぼす影響について

    福永祥子, 阿江教治, 鈴木武志, 馬建鋒, 間藤徹, 藤嶽暢英

    日本土壌肥料学会講演要旨集55   26   2009年9月

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    記述言語:日本語  

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  • A Transporter at the Node Responsible for Intervascular Transfer of Silicon in Rice

    Naoki Yamaji, Jian Feng Ma

    PLANT CELL21 ( 9 ) 2878 - 2883   2009年9月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    The concentration of essential mineral nutrients in the edible portion of plants such as grains may affect the nutritional value of these foods, while concentrations of toxic minerals in the plant are matter of food safety. Minerals taken up by the roots from soils are normally redirected at plant nodes before they are finally transported into developing seeds. However, the molecular mechanisms involved in this process have not been identified so far. Herein, we report on a transporter (Lsi6) responsible for the redirection of a plant nutrient at the node. Lsi6 is a silicon transporter in rice (Oryza sativa), and its expression in node I below the panicles is greatly enhanced when the panicle is completely emerged. Lsi6 is mainly localized at the xylem transfer cells located at the outer boundary region of the enlarged large vascular bundles in node I. Knockout of Lsi6 decreased Si accumulation in the panicles but increased Si accumulation in the flag leaf. These results suggest that Lsi6 is a transporter involved in intervascular transfer (i.e., transfer of silicon from the large vascular bundles coming from the roots to the diffuse vascular bundles connected to the panicles). These findings will be useful for selectively enhancing the accumulation of essential nutrients and reducing toxic minerals in the edible portion of cereals.

    DOI: 10.1105/tpc.109.069831

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  • Further characterization of ferric-phytosiderophore transporters ZmYS1 and HvYS1 in maize and barley

    Daisei Ueno, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY60 ( 12 ) 3513 - 3520   2009年8月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Roots of some gramineous plants secrete phytosiderophores in response to iron deficiency and take up Fe as a ferric-phytosiderophore complex through the transporter YS1 (Yellow Stripe 1). Here, this transporter in maize (ZmYS1) and barley (HvYS1) was further characterized and compared in terms of expression pattern, diurnal change, and tissue-type specificity of localization. The expression of HvYS1 was specifically induced by Fe deficiency only in barley roots, and increased with the progress of Fe deficiency, whereas ZmYS1 was expressed in maize in the leaf blades and sheaths, crown, and seminal roots, but not in the hypocotyl. HvYS1 expression was not induced by any other metal deficiency. Furthermore, in maize leaf blades, the expression was higher in the young leaf blades showing severe chlorosis than in the old leaf blades showing no chlorosis. The expression of HvYS1 showed a distinct diurnal rhythm, reaching a maximum before the onset of phytosiderophore secretion. In contrast, ZmYS1 did not show such a rhythm in expression. Immunostaining showed that ZmYS1 was localized in the epidermal cells of both crown and lateral roots, with a polar localization at the distal side of the epidermal cells. In maize leaves, ZmYS1 was localized in mesophyll cells, but not epidermal cells. These differences in gene expression pattern and tissue-type specificity of localization suggest that HvYS1 is only involved in primary Fe acquisition by barley roots, whereas ZmYS1 is involved in both primary Fe acquisition and intracellular transport of iron and other metals in maize.

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  • Effects of active silicon uptake by rice on Si-29 fractionation in various plant parts

    Jan Reent Koester, Roland Bol, Melanie J. Leng, Adrian G. Parker, Hilary J. Sloane, Jian F. Ma

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY23 ( 16 ) 2398 - 2402   2009年8月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Rice (Oryza sativa L.) accumulates large amounts of silicon which improves its growth and health due to enhanced resistance to biotic and abiotic stresses. Silicon uptake and loading to xylem in rice are predominantly active processes performed by transporters encoded by the recently identified genes Lsi1 (Si influx transporter gene) and Lsi2 (Si efflux transporter gene). Silicon deposition in rice during translocation to upper plant tissues is known to discriminate against the heavier isotopes Si-29 and Si-30, resulting in isotope fractionation within the plant. We analyzed straw and husk samples of rice mutants defective in Lsi1, Lsi2 or both for silicon content and delta Si-29 using isotope ratio mass spectrometry (IRMS) and compared these results with those for the corresponding wild-type varieties (WT). The silicon content was higher in husk than in straw. All the mutant rice lines showed clearly lower silicon content than the WT lines (4-23% Si of WT). The delta Si-29 was lower in straw and husk for the uptake defective mutant (lsi1) than for WT, albeit delta Si-29 was 0.3 parts per thousand higher in husk than in straw in both lines. The effect of defective efflux (lsi2) differed for straw and husk with higher delta Si-29 in straw, but lower delta Si-29 in husk while WT showed similar delta Si-29 in both fractions. These initial results show the potential of Si isotopes to enlighten the influence of active uptake on translocation and deposition processes in the plant. Copyright (C) 2009 John Wiley & Sons, Ltd.

    DOI: 10.1002/rcm.3971

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  • The Rice Aquaporin Lsi1 Mediates Uptake of Methylated Arsenic Species

    Ren-Ying Li, Yukiko Ago, Wen-Ju Liu, Namiki Mitani, Joerg Feldmann, Steve P. McGrath, Jian Feng Ma, Fang-Jie Zhao

    PLANT PHYSIOLOGY150 ( 4 ) 2071 - 2080   2009年8月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Pentavalent methylated arsenic (As) species such as monomethylarsonic acid [MMA(V)] and dimethylarsinic acid [DMA(V)] are used as herbicides or pesticides, and can also be synthesized by soil microorganisms or algae through As methylation. The mechanism of MMA(V) and DMA(V) uptake remains unknown. Recent studies have shown that arsenite is taken up by rice (Oryza sativa) roots through two silicon transporters, Lsi1 (the aquaporin NIP2;1) and Lsi2 (an efflux carrier). Here we investigated whether these two transporters also mediate the uptake of MMA(V) and DMA(V). MMA(V) was partly reduced to trivalent MMA(III) in rice roots, but only MMA(V) was translocated to shoots. DMA(V) was stable in plants. The rice lsi1 mutant lost about 80% and 50% of the uptake capacity for MMA(V) and DMA(V), respectively, compared with the wild-type rice, whereas Lsi2 mutation had little effect. The short-term uptake kinetics of MMA(V) can be described by a Michaelis-Menten plus linear model, with the wild type having 3.5-fold higher V-max than the lsi1 mutant. The uptake kinetics of DMA(V) were linear with the slope being 2.8-fold higher in the wild type than the lsi1 mutant. Heterologous expression of Lsi1 in Xenopus laevis oocytes significantly increased the uptake of MMA(V) but not DMA(V), possibly because of a very limited uptake of the latter. Uptake of MMA(V) and DMA(V) by wild-type rice was increased as the pH of the medium decreased, consistent with an increasing proportion of the undissociated species. The results demonstrate that Lsi1 mediates the uptake of undissociated methylated As in rice roots.

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  • Identification and Characterization of Maize and Barley Lsi2-Like Silicon Efflux Transporters Reveals a Distinct Silicon Uptake System from That in Rice

    Namiki Mitani, Yukako Chiba, Naoki Yamaji, Jian Feng Ma

    PLANT CELL21 ( 7 ) 2133 - 2142   2009年7月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Silicon (Si) uptake has been extensively examined in rice (Oryza sativa), but it is poorly understood in other gramineous crops. We identified Low Silicon Rice 2 (Lsi2)-like Si efflux transporters from two important gramineous crops: maize (Zea mays) and barley (Hordeum vulgare). Both maize and barley Lsi2 expressed in Xenopus laevis oocytes showed Si efflux transport activity. Furthermore, barley Lsi2 was able to recover Si uptake in a rice mutant defective in Si efflux. Maize and barley Lsi2 were only expressed in the roots. Expression of maize and barley Lsi2 was downregulated in response to exogenously applied Si. Moreover, there was a significant positive correlation between the ability of roots to absorb Si and the expression levels of Lsi2 in eight barley cultivars, suggesting that Lsi2 is a key Si transporter in barley. Immunostaining showed that maize and barley Lsi2 localized only at the endodermis, with no polarity. Protein gel blot analysis indicated that maize and barley Lsi2 localized on the plasma membrane. The unique features of maize and barley Si influx and efflux transporters, including their cell-type specificity and the lack of polarity of their localization in Lsi2, indicate that these crops have a different Si uptake system from that in rice.

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  • A Rice Mutant Sensitive to Al Toxicity is Defective in the Specification of Root Outer Cell Layers

    Chao-Feng Huang, Naoki Yamaji, Minoru Nishimura, Shigeyuki Tajima, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY50 ( 5 ) 976 - 985   2009年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Outer cell layers of rice roots, which comprise epidermis, exodermis and sclerenchyma, have been proposed to protect the roots from various stresses in soil. Here, we report a mutant which is defective in the specification of outer cell layers, and examined the role of these layers in Al and other metal resistance. Morphological and histochemical observations revealed that the mutant isolated based on Al sensitivity frequently showed a disordered pattern of periclinal cell division in the epidermal layers at a region close to the root apical meristem. The lateral root caps in the mutant became difficult to peel off from the epidermis, and epidermal cells became smaller and irregular with far fewer root hairs. Furthermore, some exodermal cells were transformed into additional sclerenchyma cells. However, there was no difference in the inner cell layers between the wild-type rice and the mutant. The mutant showed similar root growth to the wild-type rice in the absence of Al, but greater inhibition of root elongation by Al was found in the mutant. Morin staining showed that Al penetrated into the inner cortical cells in the mutant. Furthermore, the mutant was also sensitive to other metals including Cd and La. Taken together, our results indicate that root outer cell layers protect the roots against the toxicity of Al and other metals by preventing metal penetration into the inner cells. Genetic analysis showed that the mutant phenotypes were controlled by a single recessive gene, which was located on the short arm of rice chromosome 2.

    DOI: 10.1093/pcp/pcp050

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  • Mitigation of Arsenic Accumulation in Rice with Water Management and Silicon Fertilization

    R. Y. Li, J. L. Stroud, J. F. Ma, S. P. McGrath, F. J. Zhao

    ENVIRONMENTAL SCIENCE & TECHNOLOGY43 ( 10 ) 3778 - 3783   2009年5月

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    記述言語:英語   出版者・発行元:AMER CHEMICAL SOC  

    Rice represents a major route of As exposure in populations that depend on a rice diet. Practical measures are needed to mitigate the problem of excessive As accumulation in paddy rice. Two potential mitigation methods, management of the water regime and Si fertilization, were investigated under greenhouse conditions. Growing rice aerobically during the entire rice growth duration resulted in the leastAS accumulation. Maintaining aerobic conditions during either vegetative or reproductive stage of rice growth also decreased As accumulation in rice straw and grain significantly compared with rice grown under flooded conditions. The effect of water management regimes was consistent with the observed effect of flooding-induced arsenite mobilization in the soil solution. Aerobic treatments increased the percentage of inorganic As in grain, but the concentrations of inorganic As remained lower than in the flooded rice. Silicon fertilization decreased the total As concentration in straw and grain by 78 and 16%, respectively, even though Si addition increased As concentration in the soil solution. Silicon also significantly influenced As speciation in rice grain and husk by enhancing methylation. Silicon decreased the inorganic As concentration in grain by 59% while increasing the concentration of dimethylarsinic acid (DMA) by 33%. There were also significant differences between two rice genotypes in grain As speciation. This study demonstrated that water management, Si fertilization, and selection of rice cultivars are effective measures that can be used to reduce As accumulation in rice.

    DOI: 10.1021/es803643v

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  • HvLsi1 is a silicon influx transporter in barley

    Yukako Chiba, Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    PLANT JOURNAL57 ( 5 ) 810 - 818   2009年3月

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    記述言語:英語   出版者・発行元:WILEY  

    Most plants accumulate silicon in their bodies, and this is thought to be important for resistance against biotic and abiotic stresses; however, the molecular mechanisms for Si uptake and accumulation are poorly understood. Here, we describe an Si influx transporter, HvLsi1, in barley. This protein is homologous to rice influx transporter OsLsi1 with 81% identity, and belongs to a Nod26-like major intrinsic protein sub-family of aquaporins. Heterologous expression in both Xenopus laevis oocytes and a rice mutant defective in Si uptake showed that HvLsi1 has transport activity for silicic acid. Expression of HvLsi1 was detected specifically in the basal root, and the expression level was not affected by Si supply. There was a weak correlation between Si uptake and the expression level of HvLsi1 in eight cultivars tested. In the seminal roots, HvLsi1 is localized on the plasma membrane on the distal side of epidermal and cortical cells. HvLsi1 is also located in lateral roots on the plasma membrane of hypodermal cells. These cell-type specificity of localization and expression patterns of HvLsi1 are different from those of OsLsi1. These observations indicate that HvLsi1 is a silicon influx transporter that is involved in radial transport of Si through the epidermal and cortical layers of the basal roots of barley.

    DOI: 10.1111/j.1365-313X.2008.03728.x

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  • A Bacterial-Type ABC Transporter Is Involved in Aluminum Tolerance in Rice

    Chao Feng Huang, Naoki Yamaji, Namiki Mitani, Masahiro Yano, Yoshiaki Nagamura, Jian Feng Ma

    PLANT CELL21 ( 2 ) 655 - 667   2009年2月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Aluminum (Al) toxicity is a major factor limiting crop production in acidic soil, but the molecular mechanisms of Al tolerance are poorly understood. Here, we report that two genes, STAR1 (for sensitive to Al rhizotoxicity1) and STAR2, are responsible for Al tolerance in rice. STAR1 encodes a nucleotide binding domain, while STAR2 encodes a transmembrane domain, of a bacterial-type ATP binding cassette (ABC) transporter. Disruption of either gene resulted in hypersensitivity to aluminum toxicity. Both STAR1 and STAR2 are expressed mainly in the roots and are specifically induced by Al exposure. Expression in onion epidermal cells, rice protoplasts, and yeast showed that STAR1 interacts with STAR2 to form a complex that localizes to the vesicle membranes of all root cells, except for those in the epidermal layer of the mature zone. When expressed together in Xenopus laevis oocytes, STAR1/2 shows efflux transport activity specific for UDP-glucose. Furthermore, addition of exogenous UDP-glucose rescued root growth in the star1 mutant exposed to Al. These results indicate that STAR1 and STAR2 form a complex that functions as an ABC transporter, which is required for detoxification of Al in rice. The ABC transporter transports UDP-glucose, which may be used to modify the cell wall.

    DOI: 10.1105/tpc.108.064543

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  • Identification of Maize Silicon Influx Transporters

    Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY50 ( 1 ) 5 - 12   2009年1月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Maize (Zea mays L.) shows a high accumulation of silicon (Si), but transporters involved in the uptake and distribution have not been identified. In the present study, we isolated two genes (ZmLsi1 and ZmLsi6), which are homologous to rice influx Si transporter OsLsi1. Heterologous expression in Xenopus laevis oocytes showed that both ZmLsi1 and ZmLsi6 are permeable to silicic acid. ZmLsi1 was mainly expressed in the roots. By contrast, ZmLsi6 was expressed more in the leaf sheaths and blades. Different from OsLsi1, the expression level of both ZmLsi1 and ZmLsi6 was unaffected by Si supply. Immunostaining showed that ZmLsi1 was localized on the plasma membrane of the distal side of root epidermal and hypodermal cells in the seminal and crown roots, and also in cortex cells in lateral roots. In the shoots, ZmLsi6 was found in the xylem parenchyma cells that are adjacent to the vessels in both leaf sheaths and leaf blades. ZmLsi6 in the leaf sheaths and blades also exhibited polar localization on the side facing towards the vessel. Taken together, it can be concluded that ZmLsi1 is an influx transporter of Si, which is responsible for the transport of Si from the external solution to the root cells and that ZmLsi6 mainly functions as a Si transporter for xylem unloading.

    DOI: 10.1093/pcp/pcn110

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  • Defective Active Silicon Uptake Affects Some Components of Rice Resistance to Brown Spot

    Leandro J. Dallagnol, Fabricio A. Rodrigues, Mateus V. B. Mielli, Jian F. Ma, Lawrence E. Datnoff

    PHYTOPATHOLOGY99 ( 1 ) 116 - 121   2009年1月

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    記述言語:英語   出版者・発行元:AMER PHYTOPATHOLOGICAL SOC  

    Rice is known to accumulate high amounts of silicon (Si) in plant tissue, which helps to decrease the intensity of many economically important rice diseases. Among these diseases, brown spot, caused by the fungus Bipolaris oryzae, is one of the most devastating because it negatively affects yield and grain quality. This study aimed to evaluate the importance of active root Si uptake in rice for controlling brown spot development. Some components of host resistance were evaluated in a rice mutant, low silicon 1 (lsi1), defective in active Si uptake, and its wild-type counterpart (cv. Oochikara). Plants were inoculated with B. oryzae after growing for 35 days in a hydroponic culture amended with 0 or 2 mMol Si. The components of host resistance evaluated were incubation period (IP), relative infection efficiency (RIE), area under brown spot progress curve (AUBSPC), final lesion size (FLS), rate of lesion expansion (r), and area under lesion expansion progress curve (AULEPC). Si content from both Oochikara and lsi1 in the +Si treatment increased in leaf tissue by 219 and 178%, respectively, over the nonamended controls. Plants from Oochikara had 112% more Si in leaf tissue than plants from lsi1. The IP of brown spot from Oochikara increased approximate to 6 h in the presence of Si and the RIE, AUBSPC, FLS, r, and AULEPC were significantly reduced by 65, 75, 33, 36, and 35%, respectively. In the presence of Si, the IP increased 3 h for lsi1 but the RIE, AUBSPC, FLS, r, and AULEPC were reduced by only 40, 50, 12, 21, and 12%, respectively. The correlation between Si leaf content and IP was significantly positive but Si content was negatively correlated with RIE, AUBSPC, FLS, r, and AULEPC. Single degree-of-freedom contrasts showed that Oochikara and lsi1 supplied with Si were significantly different from those not supplied with Si for all components of resistance evaluated. This result showed that a reduced Si content in tissues of plants from lsi1 dramatically affected its basal level of resistance to brown spot, suggesting that a minimum Si concentration is needed. Consequently, the results of this study emphasized the importance of an active root Si uptake system for an increase in rice resistance to brown spot.

    DOI: 10.1094/PHYTO-99-1-0116

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  • OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice

    Kengo Yokosho, Naoki Yamaji, Daisei Ueno, Namiki Mitani, Jian Feng Ma

    PLANT PHYSIOLOGY149 ( 1 ) 297 - 305   2009年1月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Multidrug and toxic compound extrusion (MATE) transporters represent a large family in plants, but their functions are poorly understood. Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1), the closest homolog of barley (Hordeum vulgare) HvAACT1 (aluminum [Al]-activated citrate transporter 1), in terms of metal stress (iron [Fe] deficiency and Al toxicity). This gene was mainly expressed in the roots and the expression level was not affected by either Fe deficiency or Al toxicity. Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root's stele. The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice. Heterologous expression of OsFRDL1 in Xenopus oocytes showed transport activity for citrate. Immunostaining showed that OsFRDL1 was localized at the pericycle cells of the roots. On the other hand, there was no difference in the Al-induced secretion of citrate from the roots between the knockout line and the wild-type rice. Taken together, our results indicate that OsFRDL1 is a citrate transporter localized at the pericycle cells, which is necessary for efficient translocation of Fe to the shoot as a Fe-citrate complex.

    DOI: 10.1104/pp.108.128132

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  • NIP1;1, an Aquaporin Homolog, Determines the Arsenite Sensitivity of Arabidopsis thaliana

    Takehiro Kamiya, Mayuki Tanaka, Namiki Mitani, Jian Feng Ma, Masayoshi Maeshima, Toru Fujiwara

    JOURNAL OF BIOLOGICAL CHEMISTRY284 ( 4 ) 2114 - 2120   2009年1月

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    記述言語:英語   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Arsenite [As(III)] is highly toxic to organisms, including plants. Very recently, transporters in rice responsible for As(III) transport have been described (Ma, J. F., Yamaji, N., Mitani, N., Xu, X. Y., Su, Y. H., McGrath, S. P., and Zhao, F. J. (2008) Proc. Natl. Acad. Sci. U. S. A. 105, 9931-9935), but little is known about As(III) tolerance. In this study, three independent As(III)-tolerant mutants were isolated from ethyl methanesulfonate-mutagenized M2 seeds of Arabidopsis thaliana. All three mutants carried independent mutations in Nodulin 26-like intrinsic protein 1;1 (NIP1;1), a homolog of an aquaporin. Two independent transgenic lines carrying T-DNA in NIP1; 1 were highly tolerant to As(III), establishing that NIP1;1 is the causal gene of As(III) tolerance. Because an aquaglyceroporin is able to transport As(III), we measured As(III) transport activity. When expressed in Xenopus oocytes, NIP1; 1 was capable of transporting As(III). As content in the mutant plants was 30% lower than in wild-type plants. Promoter beta-glucuronidase and real-time PCR analysis showed that NIP1; 1 is highly expressed in roots, and GFP-NIP1;1 is localized to the plasma membrane. These data show that NIP1; 1 is involved in As(III) uptake into roots and that disruption of NIP1; 1 function confers As(III) tolerance to plants. NIP1;2 and NIP5;1, closely related homologs of NIP1;1, were also permeable to As(III). Although the disruption of these genes reduced the As content in plants, As(III) tolerance was not observed in nip1;2 and nip5;1 mutants. This indicates that As(III) tolerance cannot be simply explained by decreased As contents in plants.

    DOI: 10.1074/jbc.M806881200

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  • A major quantitative trait locus controlling cadmium translocation in rice (Oryza sativa)

    D. Ueno, I. Kono, K. Yokosho, T. Ando, M. Yano, J. F. Ma

    NEW PHYTOLOGIST182 ( 3 ) 644 - 653   2009年

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    The trait of low cadmium (Cd) accumulation in brown rice (Oryza sativa) is important for food safety. An effective way to reduce Cd accumulation in the grain is to control Cd transfer from the roots to the shoots.
    Here, we investigated genotypic variation in the shoot Cd concentration among 146 accessions from a rice core collection and performed a quantitative trait locus (QTL) analysis to determine the loci controlling shoot Cd accumulation. Furthermore, we physiologically characterized the two accessions used for QTL analysis.
    Large genotypic variation (13-fold) in the shoot Cd concentration was found. A major QTL was detected on chromosome 11 using a F(2) population derived from Badari Dhan (a high-Cd accession) and Shwe War (a low-Cd accession). This QTL explained 16.1% of the phenotypic variation in Cd accumulation. Furthermore, this QTL was confirmed by analysis of advanced progeny. Physiological studies showed that Badari Dhan and Shwe War did not differ in uptake of Cd by the roots, but differed greatly in the translocation of Cd from the roots to the shoots.
    Taken together, our findings suggest that the major QTL detected is responsible for the translocation of Cd from the roots to the shoots.
    New Phytologist (2009) 182: 644-653doi: 10.1111/j.1469-8137.2009.02784.x.

    DOI: 10.1111/j.1469-8137.2009.02784.x

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  • Differential gene expression of rice in response to silicon and rice blast fungus Magnaporthe oryzae

    A. M. Brunings, L. E. Datnoff, J. F. Ma, N. Mitani, Y. Nagamura, B. Rathinasabapathi, M. Kirst

    ANNALS OF APPLIED BIOLOGY155 ( 2 ) 161 - 170   2009年

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Silicon increases the resistance of rice (Oryza sativa) to the rice blast pathogen Magnaporthe oryzae. This study described the relationship between silicon and M. oryzae in terms of whole-genome gene expression. By assessing gene expression patterns in the rice cultivar Monko-to using microarray technology, the physiological basis for silicon-induced resistance was investigated. Silicon amendment resulted in the differential regulation of 221 genes in rice without being challenged with the pathogen. This means that silicon had an observable effect on rice metabolism, as opposed to playing a simple passive role in the resistance response of rice. Compared with control plants, silicon-amended rice differentially regulated 60% less genes, implying that silicon affects the rice response to rice blast infection at a transcriptional level.

    DOI: 10.1111/j.1744-7348.2009.00347.x

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  • Arsenic uptake and metabolism in plants

    F. J. Zhao, J. F. Ma, A. A. Meharg, S. P. McGrath

    NEW PHYTOLOGIST181 ( 4 ) 777 - 794   2009年

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Arsenic (As) is an element that is nonessential for and toxic to plants. Arsenic contamination in the environment occurs in many regions, and, depending on environmental factors, its accumulation in food crops may pose a health risk to humans. Recent progress in understanding the mechanisms of As uptake and metabolism in plants is reviewed here. Arsenate is taken up by phosphate transporters. A number of the aquaporin nodulin26-like intrinsic proteins (NIPs) are able to transport arsenite, the predominant form of As in reducing environments. In rice (Oryza sativa), arsenite uptake shares the highly efficient silicon (Si) pathway of entry to root cells and efflux towards the xylem. In root cells arsenate is rapidly reduced to arsenite, which is effluxed to the external medium, complexed by thiol peptides or translocated to shoots. One type of arsenate reductase has been identified, but its in planta functions remain to be investigated. Some fern species in the Pteridaceae family are able to hyperaccumulate As in above-ground tissues. Hyperaccumulation appears to involve enhanced arsenate uptake, decreased arsenite-thiol complexation and arsenite efflux to the external medium, greatly enhanced xylem translocation of arsenite, and vacuolar sequestration of arsenite in fronds. Current knowledge gaps and future research directions are also identified.

    DOI: 10.1111/j.1469-8137.2008.02716.x

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  • イネアルミニウム耐性におけるOsFRDL4の機能解析

    横正 健剛, 山地 直樹, 馬 建鋒

    日本植物生理学会年会およびシンポジウム 講演要旨集2009 ( 0 ) 436 - 436   2009年

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    出版者・発行元:日本植物生理学会  

    Multidrug and toxic compound extrusion(MATE)は排出型のトランスポーターであり生物界に広く存在することが知られている。近年、私たちはオオムギのMATE遺伝子HvAACT1がクエン酸トランスポーターであり、アルミニウムによる根圏へのクエン酸分泌に関与していることを報告した。HvAACT1のイネでの相同遺伝子は五つあり、そのうちの一つであるOsFRDL1は導管へクエン酸を輸送し、根から地上部への鉄輸送に関与することを明らかにした。本研究ではイネのもう一つの相同遺伝子、OsFRDL4の機能解析について報告する。まず、OsFRDL4のTos-17挿入株(NF)を取得し生理学的手法を用いて解析を行った。その結果、NFではOsFRDL1の破壊株のようなクロロシスや導管液中のクエン酸の低下などはみられなかった。次に、Alによる根圏へのクエン酸の分泌量を比較したところ、NFはWTに比べて著しく低下していることが明らかとなった。また破壊株のアルミニウム耐性は野生型と比べ、少し低下していた。これらのことはOsFRDL4がOsFRDL1と異なり、HvAACT1のようにAlによる根圏へのクエン酸の分泌に機能していることを示している。OsFRDL4は主に根で発現し、その発現量はHvAACT1とは異なり、アルミニウムによって数時間で100倍程度に増加していた。

    DOI: 10.14841/jspp.2009.0.0436.0

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  • 10-4 イネの鉄輸送とアルミニウム無毒化におけるOsFRDL2の役割(10.植物の微量栄養素,2009年度京都大会)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集55 ( 0 )   2009年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 11-24 イネカドミウム集積に関与する新規QTLの解析(11.植物の有害元素,2009年度京都大会)

    小山 絵美, 上野 大勢, 河野 いづみ, 横正 健剛, 安藤 露, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集55 ( 0 )   2009年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-20 ブルームレスキュウリ用カボチャ台木のケイ酸吸収特性のさらなる解析(9.植物の多量栄養素,2009年度京都大会)

    馬 建鋒, 吾郷 幸子, 山地 直樹, 三谷 奈見季, 岩崎 貢三

    日本土壌肥料学会講演要旨集55 ( 0 )   2009年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 22 カボチャ及びトマトのケイ酸吸収特性と関連遺伝子の単離(関西支部講演会,2008年度各支部会)

    馬 建鋒, 吾郷 幸子, 山地 直樹, 三谷 奈見季, 岩崎 貢三

    日本土壌肥料学会講演要旨集55 ( 0 )   2009年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 亜ヒ酸の輸送と耐性に関与するアクアポリン、NIP1;1

    神谷 岳洋, 田中 真幸, 三谷 奈見季, 馬 建鋒, 前島 正義, 藤原 徹

    日本植物生理学会年会およびシンポジウム 講演要旨集2009 ( 0 ) S0033 - S0033   2009年

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    出版者・発行元:日本植物生理学会  

    近年、アジアを中心としたヒ素(As)による土壌や水質、ひいては作物の汚染が深刻な問題となっている。作物にとってのAsは毒であり、収量低下を引き起こす。本研究では、Asの主な存在形態の一つである亜ヒ酸(As(III))の植物体内への輸送経路や耐性メカニズムを明らかにするために、シロイヌナズナのEMS処理種子を用いたスクリーニングを行った。その結果、3株のAs(III)耐性株を単離した。これらはいずれも植物のアクアグリセロポリン<I>NIP1;1</I>に変異を有していた。T-DNA挿入株も耐性を示した事から、原因遺伝子は<I>NIP1;1</I>であることが示された。<I>Xenopus</I> oocyteの発現系により、NIP1;1がAs(III)を輸送することを明らかにした。また、野生型株と<I>NIP1;1</I>破壊株のAs含量を測定したところ、野生型株に比べ<I>NIP1;1</I>破壊株では低下していた。以上の結果から、<I>NIP1;1</I>が亜ヒ酸耐性の原因遺伝子であり、輸送活性の喪失によりAs(III)耐性を付与していることが示唆された。<I>NIP1;1</I>は根で多く発現している。また、GFPを用いた解析によりNIP1;1は細胞膜の遠心側に極性を持って局在していることを明らかにした。以上のことから、NIP1;1は根におけるAs(III)の取り込みを担う主要な分子であることが示された。一方、NIP1;1の本来の生理的な機能は現在解析中である。

    DOI: 10.14841/jspp.2009.0.S0033.0

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  • Specific transporter for iron(III)-phytosiderophore complex involved in iron uptake by barley roots

    Yoshiko Murata, Emiko Harada, Kenji Sugase, Kosuke Namba, Manabu Horikawa, Jian Feng Ma, Naoki Yamaji, Daisei Ueno, Kyosuke Nomoto, Takashi Iwashita, Shoichi Kusumoto

    PURE AND APPLIED CHEMISTRY80 ( 12 ) 2689 - 2697   2008年12月

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    記述言語:英語   出版者・発行元:INT UNION PURE APPLIED CHEMISTRY  

    Iron (Fe) is an essential element for plant growth. Gramineous plants have generally developed a distinct strategy to efficiently acquire insoluble Fe, which is characterized by the synthesis and secretion of an Fe-chelating substance, phytosiderophore (PS) such as mugineic acid (MA), and by a specific uptake system for Fe(III)-PS complexes. In a previous study, we identified a gene specifically encoding an Fe(III)-PS transporter (HvYS1) in barley. This gene as well as the encoded protein is specifically expressed in the epidermal cells of the roots, and gene expression is greatly enhanced under Fe-deficient conditions. The localization and substrate specificity of HvYS1 indicate that it is a Fe(III)-PS specific transporter in barley roots. In contrast, ZmYS1, which has been reported as ail Fe-PS transporter from maize, possesses broad substrate specificity despite a high homology with HvYS1. By assessing the transport activity of a series of HvYS1-ZmYS1 chimeras, we revealed that the outer membrane loop between the 6(th) and 7(th) transmembrane regions is essential for the substrate specificity. We also achieved an efficient short-step synthesis of MA and 2'-deoxymugineic acid (DMA). Our new synthetic method enabled us to use them in a large quantity for biological studies.

    DOI: 10.1351/pac200880122689

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  • Functions and transport of silicon in plants

    J. F. Ma, N. Yamaji

    CELLULAR AND MOLECULAR LIFE SCIENCES65 ( 19 ) 3049 - 3057   2008年10月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:BIRKHAUSER VERLAG AG  

    Silicon exerts beneficial effects on plant growth and production by alleviating both biotic and abiotic stresses including diseases, pests, lodging, drought, and nutrient imbalance. Recently, two genes (Lsi1 and Lsi2) encoding Si transporters have been identified from rice. Lsi1 (low silicon 1) belongs to a Nod26-like major intrinsic protein subfamily in aquaporin, while Lsi2 encodes a putative anion transporter. Lsi1 is localized on the distal side of both exodermis and endodermis in rice roots, while Lsi2 is localized on the proximal side of the same cells. Lsi1 shows influx transport activity for Si, while Lsi2 shows efflux transport activity. Therefore, Lsi1 is responsible for transport of Si from the external solution to the root cells, whereas Lsi2 is an efflux transporter responsible for the transport of Si from the root cells to the apoplast. Coupling of Lsi1 with Lsi2 is required for efficient uptake of Si in rice.

    DOI: 10.1007/s00018-008-7580-x

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  • Transporters of arsenite in rice and their role in arsenic accumulation in rice grain

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Xiao-Yan Xu, Yu-Hong Su, Steve P. McGrath, Fang-Jie Zhao

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA105 ( 29 ) 9931 - 9935   2008年7月

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    記述言語:英語   出版者・発行元:NATL ACAD SCIENCES  

    Arsenic poisoning affects millions of people worldwide. Human arsenic intake from rice consumption can be substantial because rice is particularly efficient in assimilating arsenic from paddy soils, although the mechanism has not been elucidated. Here we report that two different types of transporters mediate transport of arsenite, the predominant form of arsenic in paddy soil, from the external medium to the xylem. Transporters belonging to the NIP subfamily of aquaporins in rice are permeable to arsenite but not to arsenate. Mutation in OsNIP2;1 (Lsi1, a silicon influx transporter) significantly decreases arsenite uptake. Furthermore, in the rice mutants defective in the silicon efflux transporter Lsi2, arsenite transport to the xylem and accumulation in shoots and grain decreased greatly. Mutation in Lsi2 had a much greater impact on arsenic accumulation in shoots and grain in field-grown rice than Lsi1. Arsenite transport in rice roots therefore shares the same highly efficient pathway as silicon, which explains why rice is efficient in arsenic accumulation. Our results provide insight into the uptake mechanism of arsenite in rice and strategies for reducing arsenic accumulation in grain for enhanced food safety.

    DOI: 10.1073/pnas.0802361105

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  • Characterization of substrate specificity of a rice silicon transporter, Lsi1

    Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY456 ( 4 ) 679 - 686   2008年7月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:SPRINGER HEIDELBERG  

    Lsi1 (OsNIP2;1) is the first silicon (silicic acid) transporter identified in plant, which belongs to the nodulin 26-like intrinsic membrane protein (NIP) subfamily. In this study, we characterized the function of this transporter by using the Xenopus laevis oocyte expression system. The transport activity of Lsi1 for silicic acid was significantly inhibited by HgCl2 but not by low temperature. Lsi1 also showed an efflux transport activity for silicic acid. The substrate specificity study showed that Lsi1 was able to transport urea and boric acid; however, the transport activity for silicic acid was not affected by the presence of equimolar urea and was decreased only slightly by boric acid. Furthermore, among the NIPs subgroup, OsNIP2;2 showed transport activity for silicic acid, whereas OsNIP1;1 and OsNIP3;1 did not. We propose that Lsi1 and its close homologues form a unique subgroup of NIP with a distinct ar/R selectivity filter, which is located in the narrowest region on the extra-membrane mouth and govern the substrate specificity of the pore.

    DOI: 10.1007/s00424-007-0408-y

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  • Reexamination of silicon effects on rice growth and production under field conditions using a low silicon mutant

    Kazunori Tamai, Jian Feng Ma

    PLANT AND SOIL307 ( 1-2 ) 21 - 27   2008年6月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Silicon (Si) is a beneficial element for healthy growth and high and sustainable production of rice, but the mode of action of the beneficial effects has not been well understood. We carried out field trials for four years at two different locations to re-examine the effects of Si on the growth and production of rice using a low silicon rice (lsi1) mutant. The mutant accumulated much lower Si at each growth stage compared with the wild-type rice (Oryza sativa L. cv Oochikara), but there was no difference in the accumulation of other nutrients including N, P, and K. Measurements at different growth stages showed that low Si in the mutant hardly affected the tiller number, chlorophyll content (SPAD value), and root growth. The plant height and shoot dry weight of the wild-type rice were slightly higher than those of the mutant at a later growth stage, but the difference was not significant between the two lines. However, grain yield was reduced by 79-98%, depending on year, due to a low Si accumulation in the mutant, which showed the largest effect of Si on rice production among all studies reported so far. Among the yield components, the percentage of filled spikelets was mostly affected, being only 13.9% of the wild-type rice in the mutant. The grain color of the mutant became brown because of excessive transpiration and infection of pathogens. These results indicate that Si increases rice yield mainly by enhancing the fertility of spikelets.

    DOI: 10.1007/s11104-008-9571-y

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  • A transporter regulating silicon distribution in rice shoots

    Naoki Yamaji, Namiki Mitatni, Jian Feng Ma

    PLANT CELL20 ( 5 ) 1381 - 1389   2008年5月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) accumulates very high concentrations of silicon (Si) in the shoots, and the deposition of Si as amorphous silica helps plants to overcome biotic and abiotic stresses. Here, we describe a transporter, Lsi6, which is involved in the distribution of Si in the shoots. Lsi6 belongs to the nodulin-26 intrinsic protein III subgroup of aquaporins and is permeable to silicic acid. Lsi6 is expressed in the leaf sheath and leaf blades as well as in the root tips. Cellular localization studies revealed that Lsi6 is found in the xylem parenchyma cells of the leaf sheath and leaf blades. Moreover, Lsi6 showed polar localization at the side facing toward the vessel. Knockdown of Lsi6 did not affect the uptake of Si by the roots but resulted in disordered deposition of silica in the shoots and increased excretion of Si in the guttation fluid. These results indicate that Lsi6 is a transporter responsible for the transport of Si out of the xylem and subsequently affects the distribution of Si in the leaf.

    DOI: 10.1105/tpc.108.059311

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  • Secretion of malate and citrate from roots is related to high Al-resistance in Lespedeza bicolor

    Xiao Ying Dong, Ren Fang Shen, Rong Fu Chen, Zhao Liang Zhu, Jian Feng Ma

    PLANT AND SOIL306 ( 1-2 ) 139 - 147   2008年4月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Lespedeza bicolor (Lespedeza bicolor Turcz. cv. Jiangxi) is a leguminous shrub that is well adapted to acid infertile soils. However, the mechanisms of aluminum resistance in this species have not been established. This study aimed to assess the possible resistance mechanisms of this plant to Al. An Al-sensitive species of Lespedeza, sericea lespedeza [Lespedeza cuneata (Dum.-Cours.) G. Don cv. Zhejiang], was used as a reference. The roots of L. bicolor secreted both malate and citrate after exposure to Al, but roots of L. cuneata did not. The secretion of organic acids from L. bicolor was specific to Al; neither 15-day P starvation nor 50 mu M lanthanum induced the secretion of these organic acid anions. Secretion of organic acid anions in L. bicolor was detected after 3-6 h exposure to Al, and the amount increased significantly after 6 h exposure, suggesting that this plant shows a pattern II-type organic acid secretion. This is supported by the finding that the secretion was significantly inhibited by a protein-synthesis inhibitor, cycloheximide. Two kinds of anion-channel inhibitors had different effects on Al-induced secretion of organic acids: 9-anthracene carboxylic acid completely inhibited secretion, phenylglyoxal had no effect. Root elongation in L. bicolor was more severely inhibited by Al in the presence of 9-anthracene carboxylic acid. All these results indicated that the secretion of malate and citrate is a specialized response to Al stress in L. bicolor roots, which might be one of the Al-resistance mechanisms in this species.

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  • Characterization of Cd translocation and identification of the Cd form in xylem sap of the Cd-hyperaccumulator Arabidopsis halleri

    Daisei Ueno, Takashi Iwashita, Fang-Jie Zhao, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY49 ( 4 ) 540 - 548   2008年4月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Arabidopsis halleriis a Cd hyperaccumulator; however, the mechanisms involved in the root to shoot translocation of Cd are not well understood. In this study, we characterized Cd transfer from the root medium to xylem in this species. Arabidopsis halleri accumulated 1,500 mg kg(1) Cd in the shoot without growth inhibition. A time-course experiment showed that the release of Cd into the xylem was very rapid; by 2 h exposure to Cd, Cd concentration in the xylem sap was 5-fold higher than that in the external solution. The concentration of Cd in the xylem sap increased linearly with increasing Cd concentration in the external solution. Cd transfer to the xylem was completely inhibited by the metabolic inhibitor carbonyl cyanide 3-chlorophenylhydrazone (CCCP). Cd concentration in the xylem sap was decreased by increasing the concentration of external Zn, but enhanced by Fe deficiency treatment. Analysis with (113)Cd-nuclear magnetic resonance (NMR) showed that the chemical shift of (113)Cd in the xylem sap was the same as that of Cd(NO(3))(2). Metal speciation with Geochem-PC also showed that Cd occurred mainly in the free ionic form in the xylem sap. These results suggest that Cd transfer from the root medium to the xylem in A. halleri is an energy-dependent process that is partly shared with Zn and/or Fe transport. Furthermore, Cd is translocated from roots to shoots in inorganic forms.

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  • Variation in root-to-shoot translocation of cadmium and zinc among different accessions of the hyperaccumulators Thlaspi caerulescens and Thlaspi praecox

    J. P. Xing, R. F. Jiang, D. Ueno, J. F. Ma, H. Schat, S. P. McGrath, F. J. Zhao

    NEW PHYTOLOGIST178 ( 2 ) 315 - 325   2008年

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING  

    Efficient root-to-shoot translocation is a key trait of the zinc/cadmium hyperaccumulators Thlaspi caerulescens and Thlaspi praecox, but the extent of variation among different accessions and the underlying mechanisms remain unclear.
    Root-to-shoot translocation of Cd and Zn and apoplastic bypass flow were determined in 10 accessions of T. caerulescens and one of T. praecox, using radiolabels Cd-109 and Zn-65. Two contrasting accessions (Pr and Ga) of T. caerulescens were further characterized for TcHMA4 expression and metal compartmentation in roots.
    Root-to-shoot translocation of Cd-109 and Zn-65 after 1 d exposure varied 4.4 to 5-fold among the 11 accessions, with a significant correlation between the two metals, but no significant correlation with uptake or the apoplastic bypass flow. The F-2 progeny from a cross between accessions from Prayon, Belgium (Pr) and Ganges, France (Ga) showed a continuous phenotype pattern and transgression. There was no significant difference in the TcHMA4 expression in roots between Pr and Ga. Compartmentation analysis showed a higher percentage of Cd-109 sequestered in the root vacuoles of Ga than Pr, the former being less efficient in translocation than the latter.
    Substantial natural variation exists in the root-to-shoot translocation of Cd and Zn, and root vacuolar sequestration may be an important factor related to this variation.

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  • イネの鉄栄養に関与するクエン酸トランスポーターOsFRDL1の機能解析

    横正 健剛, 上野 大勢, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本植物生理学会年会およびシンポジウム 講演要旨集2008 ( 0 ) 27 - 27   2008年

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    出版者・発行元:日本植物生理学会  

    近年本研究室からオオムギのアルミニウム耐性に関わる遺伝子HvAACT1が同定された。この遺伝子はmultidrug and toxin excrusion (MATE)ファミリーに属しており、アルミニウム活性型クエン酸トランスポーターである。イネにおいてHvAACT1の相同遺伝子は3つあるが、今回はもっとも相同性の高い遺伝子(アミノ酸レベルで87%)であるOsFRDL1の機能について報告する。まずはOsFRDL1のTos-17の破壊株を取得して、アルミニウムによるクエン酸の分泌量を野生型と比較した。その結果、クエン酸の分泌量に差が認められなかった。次に、異なる鉄濃度(0.2μMと10μM FeSO4)で栽培したところ、0.2μM の場合のみ、破壊株の新葉にクロロシスが観察された。地上部の鉄濃度を比較した結果、破壊株の鉄濃度は野生株より低く、導管液中の鉄濃度とクエン酸濃度も野生型の約半分であった。また破壊株の根の中心柱に鉄の沈積が観察された。この遺伝子をアフリカツメガエルの卵母細胞に発現させたところ、クエン酸の外向きの輸送活性が認められた。この遺伝子は主に根で発現していて、その発現量は鉄の有無によってほとんど影響を受けなかった。これらの結果はOsFRDL1はオオムギのHvAACT1とは異なり、中心柱へクエン酸を輸送するために必要なトランスポーターであることを示している。このトランスポーターは鉄をクエン酸との錯体の形態で地上部に輸送するのに必要である。

    DOI: 10.14841/jspp.2008.0.0027.0

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  • P9-17 イネの地上部へのカドミウム集積に関与するQTL解析(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)

    上野 大勢, 矢野 昌裕, 河野 いつみ, 安藤 露, 横正 健剛, 馬 建鋒

    日本土壌肥料学会講演要旨集54 ( 0 )   2008年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P9-30 カボチャ2品種のケイ酸吸収特性の解析(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)

    吾郷 幸子, 三谷 奈見季, 山地 直樹, 岩崎 貢三, 馬 建鋒

    日本土壌肥料学会講演要旨集54 ( 0 )   2008年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P9-31 異なるイネ科植物由来のケイ酸トランスポーター特性の比較解析(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)

    三谷 奈見季, 千葉 由佳子, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集54 ( 0 )   2008年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-23 植物の金属ストレスにおけるMATE遺伝子の機能解析(9.植物の無機栄養,2008年度愛知大会)

    横正 健剛, 上野 大勢, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集54 ( 0 )   2008年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-26 イネのヒ素吸収に関与するトランスポーターの同定(9.植物の無機栄養,2008年度愛知大会)

    馬 建鋒, 山地 直樹, 三谷 奈見季, Zhao Fangjie

    日本土壌肥料学会講演要旨集54 ( 0 )   2008年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • The genetic basic and fine-mapping of a stable quantitative-trait loci for aluminium tolerance in rice

    Y. Xue, L. Jiang, N. Su, J. K. Wang, P. Deng, J. F. Ma, H. Q. Zhai, J. M. Wan

    PLANTA227 ( 1 ) 255 - 262   2007年12月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Aluminium (Al) toxicity is a primary cause of low rice productivity in acid soils. We have mapped a number of quantitative-trait loci (QTL) controlling Al tolerance in a recombinant inbred line population derived from a cross between the tolerant japonica cultivar Asominori and the sensitive indica cultivar IR24. Tolerance was assessed on the basis of relative root elongation. QTL were detected on chromosomes 1, 9, and 11, with the percentages of phenotypic variance explained ranging from 13.5 to 17.7%. Alleles from Asominori at all three QTL were associated with increased Al tolerance. qRRE-9 is expressed both in the genetic background of IR24 and in an Asominori/IR24-mixed background. qRRE-9 was reduced to the single recessive Mendelian factor Alt-9. High-resolution genetic and physical maps were constructed for Alt-9 in a BC3F2 population of 1,043 individuals. Alt-9 maps between RM24702 and ID47-2 on chromosome 9, and co-segregates with RM5765.

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  • Mutation in nicotianamine aminotransferase stimulated the Fe(II) acquisition system and led to iron accumulation in Rice

    Longjun Cheng, Fang Wang, Huixia Shou, Fangliang Huang, Luqing Zheng, Fei He, Jinhui Li, Fang-Jie Zhao, Daisei Ueno, Jian Feng Ma, Ping Wu

    PLANT PHYSIOLOGY145 ( 4 ) 1647 - 1657   2007年12月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Higher plants acquire iron (Fe) from the rhizosphere through two strategies. Strategy II, employed by graminaceous plants, involves secretion of phytosiderophores (eg. deoxymugineic acid in rice [Oryza sativa]) by roots to solubilize Fe(III) in soil. In addition to taking up Fe in the form of Fe(III)-phytosiderophore, rice also possesses the strategy I-like system that may absorb Fe(II) directly. Through mutant screening, we isolated a rice mutant that could not grow with Fe(III)-citrate as the sole Fe source, but was able to grow when Fe(II)-EDTA was supplied. Surprisingly, the mutant accumulated more Fe and other divalent metals in roots and shoots than the wild type when both were supplied with EDTA-Fe(II) or grown under waterlogged field conditions. Furthermore, the mutant had a significantly higher concentration of Fe in both unpolished and polished grains than the wild type. Using the map-based cloning method, we identified a point mutation in a gene encoding nicotianamine aminotransferase (NAAT1), which was responsible for the mutant phenotype. Because of the loss of function of NAAT1, the mutant failed to produce deoxymugineic acid and could not absorb Fe(III) efficiently. In contrast, nicotianamine, the substrate for NAAT1, accumulated markedly in roots and shoots of the mutant. Microarray analysis showed that the expression of a number of the genes involved in Fe(II) acquisition was greatly stimulated in the naat1 mutant. Our results demonstrate that disruption of deoxymugineic acid biosynthesis can stimulate Fe(II) acquisition and increase iron accumulation in rice.

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  • Genotypic difference in silicon uptake and expression of silicon transporter genes in rice

    Jian Feng Ma, Naoki Yamaji, Kazunori Tamai, Namiki Mitani

    PLANT PHYSIOLOGY145 ( 3 ) 919 - 924   2007年11月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) is a highly silicon (Si)-accumulating species that shows genotypic differences in Si accumulation. We investigated the physiological and molecular mechanisms involved in the genotypic difference in Si uptake between the japonica var. Nipponbare and the indica var. Kasalath. Both the Si concentration in the shoot and the Si uptake per root dry weight were higher in Nipponbare than in Kasalath grown in either soil or nutrient solution. The Si uptake by a single root was also higher in Nipponbare than in Kasalath. A kinetics study showed that Nipponbare and Kasalath had a similar K-m value, whereas the V-max was higher in Nipponbare. The expression of two Si transporter genes (Low silicon rice 1 [Lsi1] and Lsi2) investigated using real-time reverse transcription polymerase chain reaction revealed higher expression of both genes in Nipponbare than in Kasalath. Immunostaining with Lsi1 and Lsi2 antibodies revealed a similar pattern of subcellular localization of these two Si transporters in both varieties; Lsi1 and Lsi2 were localized at the distal and proximal sides, respectively, of both exodermis and endodermis of the roots. These results revealed that the genotypic difference in the Si accumulation results from the difference in abundance of Si transporters in rice roots.

    DOI: 10.1104/pp.107.107599

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  • An aluminum-activated citrate transporter in barley

    Jun Furukawa, Naoki Yamaji, Hua Wang, Namiki Mitani, Yoshiko Murata, Kazuhiro Sato, Maki Katsuhara, Kazuyoshi Takeda, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY48 ( 8 ) 1081 - 1091   2007年8月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Soluble ionic aluminum (Al) inhibits root growth and reduces crop production on acid soils. Al-resistant cultivars of barley (Hordeum vulgare L.) detoxify Al by secreting citrate from the roots, but the responsible gene has not been identified yet. Here, we identified a gene (HvAACT1) responsible for the Al-activated citrate secretion by fine mapping combined with microarray analysis, using an Al-resistant cultivar, Murasakimochi, and an Al-sensitive cultivar, Morex. This gene belongs to the multidrug and toxic compound extrusion (MATE) family and was constitutively expressed mainly in the roots of the Al-resistant barley cultivar. Heterologous expression of HvAACTI in Xenopus oocytes showed efflux activity for 14 C-labeled citrate, but not for malate. Twoelectrode voltage clamp analysis also showed transport activity of citrate in the HvAACT1-expressing oocytes in the presence of Al. Overexpression of this gene in tobacco enhanced citrate secretion and Al resistance compared with the wild-type plants. Transiently expressed green fluorescent protein-tagged HvAACT1 was localized at the plasma membrane of the onion epidermal cells, and immunostaining showed that HvAACT1 was localized in the epidermal cells of the barley root tips. A good correlation was found between the expression of HvAACT1 and citrate secretion in 10 barley cultivars differing in Al resistance. Taken together, our results demonstrate that HvAACT1 is an Al-activated citrate transporter responsible for Al resistance in barley.

    DOI: 10.1093/pcp/pcm091

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  • An efflux transporter of silicon in rice

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Kazunori Tamai, Saeko Konishi, Toru Fujiwara, Maki Katsuhara, Masahiro Yano

    NATURE448 ( 7150 ) 209 - U12   2007年7月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Silicon is an important nutrient for the optimal growth and sustainable production of rice(1-4). Rice accumulates up to 10% silicon in the shoot, and this high accumulation is required to protect the plant from multiple abiotic and biotic stresses(1-5). A gene, Lsi1, that encodes a silicon influx transporter has been identified in rice(6). Here we describe a previously uncharacterized gene, low silicon rice 2 (Lsi2), which has no similarity to Lsi1. This gene is constitutively expressed in the roots. The protein encoded by this gene is localized, like Lsi1, on the plasma membrane of cells in both the exodermis and the endodermis, but in contrast to Lsi1, which is localized on the distal side, Lsi2 is localized on the proximal side of the same cells. Expression of Lsi2 in Xenopus oocytes did not result in influx transport activity for silicon, but preloading of the oocytes with silicon resulted in a release of silicon, indicating that Lsi2 is a silicon efflux transporter. The identification of this silicon transporter revealed a unique mechanism of nutrient transport in plants: having an influx transporter on one side and an efflux transporter on the other side of the cell to permit the effective transcellular transport of the nutrients.

    DOI: 10.1038/nature05964

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  • Sorghum roots are inefficient in uptake of EDTA-chelated lead

    Yong Xu, Naoki Yamaji, Renfang Shen, Jian Feng Ma

    ANNALS OF BOTANY99 ( 5 ) 869 - 875   2007年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Background and Aims Ethylene diamine tetraacetic acid (EDTA)-assisted phytoremediation has been developed to clean up lead (Pb)-contaminated soil; however, the mechanism responsible for the uptake of EDTA-Pb complex is not well understood. In this study, the accumulation process of Pb from EDTA-Pb is characterized in comparison to ionic Pb [Pb(NO3)(2)] in sorghum (Sorghum bicolor).Methods Sorghum seedlings were exposed to a 0.5 mm CaCl2 (pH 5.0) solution containing 0, 1 mm Pb(NO3)(2) or EDTA-Pb complexes at a molar ratio of 1:0.5, 1:1, 1:2 and 1:4 (Pb:EDTA). The root elongation of sorghum at different ratios of Pb:EDTA was measured. Xylem sap was collected after the stem was severed at different times. The concentration of Pb in the shoots and roots were determined by an atomic absorption spectrometer. In addition, the roots were stained with Fluostain I for observation of the root structure.Key Results Lead accumulation in the shoots of the plants exposed to EDTA-Pb at 1:1 ratio was only one-fifth of that exposed to ionic Pb at the same concentration. Lead accumulation decreased when transpiration was suppressed. The concentration of Pb in the xylem sap from the EDTA-Pb-treated plants was about 1/25 000 of that in the external solution. Root elongation was severely inhibited by ionic Pb, but not by EDTA-Pb at a 1:1 ratio. Root staining showed that a physiological barrier was damaged in the roots exposed to ionic Pb, but not in the roots exposed to EDTA-Pb.Conclusions All these results suggest that sorghum roots are inefficient in uptake of EDTA-chelated Pb and that enhanced Pb accumulation from ionic Pb was attributed to the damaged structure of the roots.

    DOI: 10.1093/aob/mcm038

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  • Spatial distribution and temporal variation of the rice silicon transporter Lsi1

    Naoki Yamaji, Jian Feng Ma

    PLANT PHYSIOLOGY143 ( 3 ) 1306 - 1313   2007年3月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) is a typical silicon (Si) accumulator and requires a large amount of Si for high-yield production. Recently, a gene (Low silicon rice1 [Lsi1]) encoding a Si transporter was identified in rice roots. Here, we characterized Lsi1 in terms of spatial distribution and temporal variation using both physiological and molecular approaches. Results from a multicompartment transport box experiment showed that the major site for Si uptake was located at the basal zone (> 10 mm from the root tip) of the roots rather than at the root tips (< 10 mm from the root tip). Consistent with the Si uptake pattern, Lsi1 expression and distribution of the Lsi1 protein were found only in the basal zone of roots. In the basal zones of the seminal, crown, and lateral roots, the Lsi1 protein showed a polar localization at the distal side of both the exodermis and endodermis, where the Casparian bands are formed. This indicates that Lsi1 is required for the transport of Si through the cells of the exodermis and endodermis. Expression of Lsi1 displayed a distinct diurnal pattern. Furthermore, expression was transiently enhanced around the heading stage, which coincides with a high Si requirement during this growth stage. Expression was down-regulated by dehydration stress and abscisic acid, suggesting that expression of Lsi1 may be regulated by abscisic acid.

    DOI: 10.1104/pp.106.093005

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  • Phytoremediation of Pb contaminated soil with polymer-coated EDTA

    Masaru Shibata, Takayuki Konno, Ryo Akaike, Yong Xu, Renfang Shen, Jian Feng Ma

    PLANT AND SOIL290 ( 1-2 ) 201 - 208   2007年1月

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    記述言語:英語   出版者・発行元:SPRINGER  

    EDTA-assisted phytoextraction of lead (Pb) has been developed, but concerns have arisen due to the possibility of leaching of both Pb and EDTA to ground water caused by uncontrolled release. We developed five types of controlled-release EDTA (polymer-coated EDTA) by coating the EDTA with a polyolefin polymer. A test of the release rate showed that the duration for the release of 75% of total EDTA ranged from 3 to 210 days. A pot experiment was conducted to compare the effect of these polymer-coated EDTA and non-coated EDTA on the concentrations of Pb and EDTA in soil solution, and Pb accumulation in sorghum (Sorghum bicolor L. cv. EARLY SUMAC) in a Pb-contaminated soil. One of the polymer-coated EDTAs, C-EDTA-4, with a release period of 80 days proved to be the best in decreasing Pb and EDTA concentrations in soil solution, and increasing Pb accumulation in sorghum shoots compared to the direct application of EDTA. Our results suggest that polymer-coated EDTA has a potential for phytoextraction of Pb with a reduced environmental risk.

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  • Identification of two novel phytosiderophores secreted by perennial grasses

    D. Ueno, A. D. Rombola, T. Iwashita, K. Nomoto, J. F. Ma

    NEW PHYTOLOGIST174 ( 2 ) 304 - 310   2007年

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING  

    It has been suggested that some perennial grasses secrete phytosiderophores in response to iron (Fe) deficiency, but the compounds have not been identified. Here, we identified and characterized the phytosiderophores secreted by two perennial grasses, Lolium perenne cv. Tove and Poa pratensis cv. Baron.
    Root exudates were collected from the roots of Fe-deficient grasses and then purified with various chromatographies. The structure of the purified compounds was determined using both nuclear magnetic resonance and fast atom bombardment mass spectrometry.
    Both species secreted phytosiderophores in response to Fe deficiency, and the amount of phytosiderophores secreted increased with the development of Fe deficiency. The type of phytosiderophores secreted differed with plant species; L. perenne cv. Tove secreted 3-epihydroxy-2'-deoxymugineic acid (epiHDMA), 2'-deoxymugineic acid (DMA) and an unknown compound, whereas P. pratensis cv. Baron secreted DMA, avenic acid A (AVA) and an unknown compound.
    Purification and subsequent analysis with nuclear magnetic resonance and mass led to identification of the two novel phytosiderophores; 3-hydroxy-2'-deoxymugineic acid (HDMA) from L. perenne, and 2'-hydroxyavenic acid A (HAVA) from P. pratensis. Both novel phytosiderophores have similar chelating activity to known phytosiderophores.

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  • イネのケイ素トランスポーター.

    馬 建鋒, 山地直樹, 三谷奈見季

    タンパク質核酸酵素52   1849 - 1856   2007年

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  • Syndrome of aluminum toxicity and diversity of aluminum resistance in higher plants

    Jian Feng Ma

    SURVEY OF CELL BIOLOGY264   225 - +   2007年

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    記述言語:英語   出版者・発行元:ELSEVIER ACADEMIC PRESS INC  

    Aluminum (Al) is the most abundant metal in the earth's crust, while its soluble ionic form (Al3+) shows phytotoxicity, which is characterized by a rapid inhibition of root elongation. Aluminum targets multiple cellular sites by binding, resulting in disrupted structure and/or functions of the cell wait, plasma membrane, signal transduction pathway, and Ca homeostasis. On the other hand, some plant species have evolved mechanisms to cope with At toxicity both externally and internally. The well-documented mechanisms for external detoxification of At include the release of organic acid anions from roots and alkalination of the rhizosphere. Genes encoding transporters for Al-induced secretion of organic acid anions have been identified and characterized. Recent studies show that ABC transporters are involved in At resistance. The internal detoxification of Al in Al-accumulating plants is achieved by the formation of nontoxic Al complexes with organic acids or other chelators and sequestration of these complexes in the vacuoles. In some plant species, Al shows beneficial effects on plant growth under particular conditions, although the exact mechanisms for these effects are unknown.

    DOI: 10.1016/S0074-7696(07)64005-4

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  • Strategies of plants to adapt to mineral stresses in problem soils

    Syuntaro Hiradate, Jian Feng Ma, Hideaki Matsumoto

    ADVANCES IN AGRONOMY, VOL 9696   65 - 132   2007年

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    記述言語:英語   出版者・発行元:ELSEVIER ACADEMIC PRESS INC  

    DOI: 10.1016/S0065-2113(07)96004-6

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  • 9-6 オオムギにおけるアルミニウムによるクエン酸分泌に関与する遺伝子の単離と機能解析(9.植物の無機栄養,2007年度東京大会)

    古川 純, 山地 直樹, 王 華, 三谷 奈見季, 村田 佳子, 佐藤 和広, 且原 真木, 武田 和義, 馬 建鋒

    日本土壌肥料学会講演要旨集53 ( 0 )   2007年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P9-40 大麦におけるケイ酸吸収機構の解析(9.植物の無機栄養,2007年度東京大会)

    千葉 由佳子, 三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集53 ( 0 )   2007年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P9-41 イネのケイ酸輸送体と吸収・集積機構(9.植物の無機栄養,2007年度東京大会)

    山地 直樹, 三谷 奈見季, 玉井 一規, 馬 建鋒

    日本土壌肥料学会講演要旨集53 ( 0 )   2007年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-41 トウモロコシ由来のケイ酸吸収遺伝子の単離と解析(9.植物の無機栄養,2007年度東京大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集53 ( 0 )   2007年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • Genetic dissection of silicon uptake ability in rice (Oryza sativa L.)

    Q. -S. Wu, X. -Y. Wan, N. Su, Z. -J. Cheng, J. -K. Wang, C. -L. Lei, X. Zhang, L. Jiang, J. -F. Ma, J. -M. Wan

    PLANT SCIENCE171 ( 4 ) 441 - 448   2006年10月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    The adequate presence of silicon (Si) in rice plants can enhance their yield and improve their tolerance to various biotic and abiotic stresses. In this study Si uptake abilities were compared between the japonica rice cultivar (cv.) Kinmaze and the indica rice cv. DV85 under three Si concentrations (0.16, 0.4, and 1.6 mM) at different time points from 1 to 12 h. The results showed that the phenotypic values of two traits-Si uptake by individual plants (SP, Si uptake by all roots of a plant) and Si uptake per unit root dry weight (SR = SP/root dry weight)-of Kinmaze were significantly higher than those of DV85 (P &lt; 0.01). Meanwhile, a kinetic study indicated that the Si transporters in Kinmaze and DV85 had the same affinity for silicic acid, but with different V-max values, indicating that Kinmaze had more Si transporters in the roots than DV85. This may be the main reason for the difference in Si uptake ability between Kinmaze and DV85. In addition, a mapping population consisting of 81 recombinant inbred lines (RILs) derived from the cross between Kinmaze and DV85 was used to detect quantitative trait loci (QTLs) underlying SP and SR. The RILs follow a continuous one-peak distribution and show transgressive segregation in both directions for SP, SR, and root dry weight (RDW). Three QTLs for SP, four for SR, and three for RDW were detected. This can explain 7.16-17.15% of the phenotypic variation (PVE). Thus, the results obtained in this study provide a better understanding of the mechanism of rice Si uptake ability and the basis for fine-mapping the genes involved. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.plantsci.2006.05.001

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  • QTL analysis of aluminum resistance in rice (Oryza sativa L.)

    Y. Xue, J. M. Wan, L. Jiang, L. L. Liu, N. Su, H. Q. Zhai, J. F. Ma

    PLANT AND SOIL287 ( 1-2 ) 375 - 383   2006年9月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Aluminum (Al) toxicity is considered as one of the primary causes of low-rice productivity in acid soils. In the present study, quantitative trait loci (QTLs) controlling Al resistance based on relative root elongation (RRE) were dissected using a complete linkage map and a recombinant inbred lines (RILs) derived from a cross of Al-tolerant japonica cultivar Asominori (Oryza sativa L.) and Al sensitive indica cultivar IR24 (O. sativa L.). A total of three QTLs (qRRE-1, qRRE-9, and qRRE-11) were detected on chromosomes 1, 9, and 11 with LOD score ranging from 2.64 to 3.60 and the phenotypic variance explained from 13.5 to 17.7%. The Asominori alleles were all associated with Al resistance at all the three QTLs. The existence of these QTLs was confirmed using Asominori chromosome segment substitution lines (CSSLs) in IR24 genetic background (IAS). By QTL comparative analysis, the two QTLs (qRRE-1 and qRRE-9) on chromosomes 1 and 9 appeared to be consistent among different rice populations while qRRE-11 was newly detected and syntenic with a major Al resistance gene on chromosome 10 of maize. This region may provide an important case for isolating genes responsible for different mechanisms of Al resistance among different cereals. These results also provide the possibilities of enhancing Al resistance in rice breeding program by marker-assisted selection (MAS) and pyramiding QTLs.

    DOI: 10.1007/s11104-006-9086-3

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  • Silicon uptake and accumulation in higher plants

    Jian Feng Ma, Naoki Yamaji

    TRENDS IN PLANT SCIENCE11 ( 8 ) 392 - 397   2006年8月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:ELSEVIER SCIENCE LONDON  

    Silicon (Si) accumulation differs greatly between plant species because of differences in Si uptake by the roots. Recently, a gene encoding a Si uptake transporter in rice, a typical Si-accumulating plant, was isolated. The beneficial effects of Si are mainly associated with its high deposition in plant tissues, enhancing their strength and rigidity. However, Si might play an active role in enhancing host resistance to plant diseases by stimulating defense reaction mechanisms. Because many plants are not able to accumulate Si at high enough levels to be beneficial, genetically manipulating the Si uptake capacity of the root might help plants to accumulate more Si and, hence, improve their ability to overcome biotic and abiotic stresses.

    DOI: 10.1016/j.tplants.2006.06.007

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  • Response of rice (Oryza sativa) with root surface iron plaque under aluminium stress

    Rong Fu Chen, Ren Fang Shen, Pei Gu, Xiao Ying Dong, Chang Wen Du, Jian Feng Ma

    ANNALS OF BOTANY98 ( 2 ) 389 - 395   2006年8月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Background and Aims Rice (Oryza sativa) is an aquatic plant with a characteristic of forming iron plaque on its root surfaces. It is considered to be the most Al-tolerant species among the cereal crops. The objective of this study was to determine the effects of root surface iron plaque on Al translocation, accumulation and the change of physiological responses under Al stress in rice in the presence of iron plaque. 9Methods The japonica variety rice, Koshihikari, was used in this study and was grown hydroponically in a growth chamber. Iron plaque was induced by exposing the rice roots to 30 mg L-1 ferrous iron either as Fe(II)-EDTA in nutrient solution (6 d, Method I) or as FeSO4 in water solution (12 h, Method II). Organic acid in root exudates was retained in the anion-exchange resin and eluted with 2M HCl, then analysed by high-performance liquid chromatography (HPLC) after proper pre-treatment. Fe and Al in iron plaque were extracted with DCB (dithionite-citrate-bicarbonate) solution.Key Results and Conclusions Both methods (I and II) could induce the formation of iron plaque on rice root surfaces. The amounts of DCB-extractable Fe and Al on root surfaces were much higher in the presence of iron plaque than in the absence of iron plaque. Al contents in root tips were significantly decreased with iron plaque; translocation of At from roots to shoots was significantly reduced with iron plaque. Al-induced secretion of citrate was observed and iron plaque could greatly depress this citrate secretion. These results suggested that iron plaque on rice root surfaces can be a sink to sequester Al onto the root surfaces and Fe ions can pre-saturate Al-binding sites in root tips, which protects the rice root tips from suffering Al stress to a certain extent.

    DOI: 10.1093/aob/mcl110

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  • Buckwheat accumulates aluminum in leaves but not in seeds

    Ren Fang Shen, Rong Fu Chen, Jian Feng Ma

    PLANT AND SOIL284 ( 1-2 ) 265 - 271   2006年6月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Buckwheat (Fagopyrum esculentum Moench. cv Jianxi) is highly resistant to Al stress and is known to be an Al-accumulator. Pot experiments were carried out in a greenhouse to investigate the accumulation of Al in leaves and seeds of buckwheat. Plants were grown for 12 weeks in a strong acid soil amended with or without CaCO3 at a rate of 1 g kg(-1) soil. Old leaves accumulated as much as 10 g kg(-1) Al of dry weight when the plants were grown in the acid soil, while the Al concentrations in leaves immediately adjacent to seeds, seed coats, and embryos were, on average, 4516, 41.2 and 7.7 mg kg(-1) stop, respectively. The Al concentration significantly decreased in leaves when the plants were grown in the limed soil, and the Al concentrations in leaves immediately adjacent to seeds, seed coats, and embryos were, on average, 1586, 21.3 and 3.1 mg kg(-1), respectively. These results show that seeds accumulate much less Al than buckwheat leaves. The underlying mechanisms are discussed.

    DOI: 10.1007/s11104-006-0044-x

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  • A specific transporter for iron(III)-phytosiderophore in barley roots

    Y Murata, JF Ma, N Yamaji, D Ueno, K Nomoto, T Iwashita

    PLANT JOURNAL46 ( 4 ) 563 - 572   2006年5月

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING  

    Iron acquisition of graminaceous plants is characterized by the synthesis and secretion of the iron-chelating phytosiderophore, mugineic acid (MA), and by a specific uptake system for iron(III)-phytosiderophore complexes. We identified a gene specifically encoding an iron-phytosiderophore transporter (HvYS1) in barley, which is the most tolerant species to iron deficiency among graminaceous plants. HvYS1 was predicted to encode a polypeptide of 678 amino acids and to have 72.7% identity with ZmYS1, a first protein identified as an iron(III)-phytosiderophore transporter in maize. Real-time RT-PCR analysis showed that the HvYS1 gene was mainly expressed in the roots, and its expression was enhanced under iron deficiency. In situ hybridization analysis of iron-deficient barley roots revealed that the mRNA of HvYS1 was localized in epidermal root cells. Furthermore, immunohistological staining with anti-HvYS1 polyclonal antibody showed the same localization as the mRNA. HvYS1 functionally complemented yeast strains defective in iron uptake on media containing iron(III)-MA, but not iron-nicotianamine (NA). Expression of HvYS1 in Xenopus oocytes showed strict specificity for both metals and ligands: HvYS1 transports only iron(III) chelated with phytosiderophore. The localization and substrate specificity of HvYS1 is different from those of ZmYS1, indicating that HvYS1 is a specific transporter for iron(III)-phytosiderophore involved in primary iron acquisition from soil in barley roots.

    DOI: 10.1111/j.1365-313X.2006.02714.x

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  • A silicon transporter in rice

    JF Ma, K Tamai, N Yamaji, N Mitani, S Konishi, M Katsuhara, M Ishiguro, Y Murata, M Yano

    NATURE440 ( 7084 ) 688 - 691   2006年3月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Silicon is beneficial to plant growth and helps plants to overcome abiotic and biotic stresses by preventing lodging ( falling over) and increasing resistance to pests and diseases, as well as other stresses(1-3). Silicon is essential for high and sustainable production of rice(4), but the molecular mechanism responsible for the uptake of silicon is unknown. Here we describe the Low silicon rice 1 (Lsi1) gene, which controls silicon accumulation in rice, a typical silicon-accumulating plant. This gene belongs to the aquaporin family(5) and is constitutively expressed in the roots. Lsi1 is localized on the plasma membrane of the distal side of both exodermis and endodermis cells, where casparian strips are located. Suppression of Lsi1 expression resulted in reduced silicon uptake. Furthermore, expression of Lsi1 in Xenopus oocytes showed transport activity for silicon only. The identification of a silicon transporter provides both an insight into the silicon uptake system in plants, and a new strategy for producing crops with high resistance to multiple stresses by genetic modification of the root's silicon uptake capacity.

    DOI: 10.1038/nature04590

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  • A silicon transporter in rice

    JF Ma, K Tamai, N Yamaji, N Mitani, S Konishi, M Katsuhara, M Ishiguro, Y Murata, M Yano

    NATURE440 ( 7084 ) 688 - 691   2006年3月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Silicon is beneficial to plant growth and helps plants to overcome abiotic and biotic stresses by preventing lodging ( falling over) and increasing resistance to pests and diseases, as well as other stresses(1-3). Silicon is essential for high and sustainable production of rice(4), but the molecular mechanism responsible for the uptake of silicon is unknown. Here we describe the Low silicon rice 1 (Lsi1) gene, which controls silicon accumulation in rice, a typical silicon-accumulating plant. This gene belongs to the aquaporin family(5) and is constitutively expressed in the roots. Lsi1 is localized on the plasma membrane of the distal side of both exodermis and endodermis cells, where casparian strips are located. Suppression of Lsi1 expression resulted in reduced silicon uptake. Furthermore, expression of Lsi1 in Xenopus oocytes showed transport activity for silicon only. The identification of a silicon transporter provides both an insight into the silicon uptake system in plants, and a new strategy for producing crops with high resistance to multiple stresses by genetic modification of the root's silicon uptake capacity.

    DOI: 10.1038/nature04590

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  • イネのケイ酸吸収機構.

    山地直樹, 馬 建鋒

    化学と生物44:453-458   2006年

  • イネのケイ酸輸送体.

    馬 建鋒, 山地直樹, 三谷奈見季, 玉井一規

    細胞工学25:778-779   2006年

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  • 9-66 イネケイ酸吸収遺伝子Lsi1の輸送特性の解析(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

    三谷 奈見季, 且原 真木, 馬 建鋒

    日本土壌肥料学会講演要旨集52 ( 0 )   2006年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • ケイ酸吸収遺伝子<I>Lsi1の機能解析</I>

    三谷 奈見季, 山地 直樹, 玉井 一規, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本植物生理学会年会およびシンポジウム 講演要旨集2006 ( 0 ) 147 - 147   2006年

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    出版者・発行元:日本植物生理学会  

    前年度の年会ではイネのケイ酸吸収遺伝子&lt;I&gt;Lsi1&lt;/I&gt;の単離について報告したが、今回はこの遺伝子の機能解析の結果を報告する。まずは発現部位を定量RT-PCRで解析した。その結果、この遺伝子は主に根に構成的に発現していることがわかった。またこの遺伝子の発現量はケイ酸の有無によって変動し、ケイ酸が十分ある条件と比べ、ない場合は&lt;I&gt;Lsi1&lt;/I&gt;の発現量が4倍増加した。&lt;I&gt;Lsi1&lt;/I&gt;によってコードされたタンパク質の局在性を調べるために、&lt;I&gt;Lsi1&lt;/I&gt;のプロモーター及び&lt;I&gt;Lsi1&lt;/I&gt;遺伝子とGFPを連結させたコンストラクトを作成し、組み換えイネを作出した。その結果、Lsi1タンパク質は主に主根と側根に存在し、根毛にないことを明らかにした。また細胞局在性を調べると、Lsi1は根の外皮と内皮細胞に局在していることが明らかとなった。さらに、Lsi1の抗体を作成し、抗体染色した結果、同じく外皮と内皮、しかも外側の細胞膜に局在していることがわかった。RNA干渉法(RNAi)で&lt;I&gt;Lsi1&lt;/I&gt;の発現を抑制した形質転換植物体では、ケイ酸吸収量も減少した。さらに、&lt;I&gt;Lsi1&lt;/I&gt;にコードされたタンパクにケイ酸の輸送活性があるか調べるために、&lt;I&gt;Lsi1&lt;/I&gt;のcRNAを作成し、それをアフリカツメガエルの卵母細胞に注入し、ケイ酸の輸送活性を測定した。その結果、水のみを注入したコントロールに比べ、野生型のcRNAを注入した場合、ケイ酸の輸送活性は3倍増加した。また変異体のcRNAを注入した場合、ケイ酸の輸送活性は野生型と比べ、かなり低かった。

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  • 9-67 イネケイ酸吸収関連遺伝子lsi2の解析(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

    玉井 一規, 山地 直樹, 三谷 奈見季, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集52 ( 0 )   2006年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • Physiological mechanisms of at resistance in higher plants

    JF Ma

    SOIL SCIENCE AND PLANT NUTRITION51 ( 5 ) 609 - 612   2005年9月

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    記述言語:英語   出版者・発行元:JAPANESE SOC SOIL SCIENCE PLANT NUTRITION  

    Aluminum (Al) ion is toxic to plant growth, while the resistance to Al toxicity varies widely among plant species. Accumulating evidence has shown that organic acids play an important role in both internal and external detoxification of Al. Two patterns of Al-induced secretion of organic acid anions have been characterized in the external detoxification. Involvement of ABA or protein phosphorylation in the activation of anion channel in Pattern I and less contribution of organic acid metabolism in Pattern 11 have been reported. Recently, gene or quantitative trait locus (QTL) for Al-activated secretion of organic acid anions has been identified. Other mechanisms of Al resistance are also reported such as pH increase in the rhizosphere. The formation of a non-toxic Al complex with organic acids or other chelators and sequestering these complexes in the vacuoles play an important role in internal detoxification of Al in Al-accumulating plants.

    DOI: 10.1111/j.1747-0765.2005.tb00074.x

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  • Identification of the form of Cd in the leaves of a superior Cd-accumulating ecotype of Thlaspi caerulescens using Cd-113-NMR

    D Ueno, JF Ma, T Iwashita, FJ Zhao, SP McGrath

    PLANTA221 ( 6 ) 928 - 936   2005年8月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Thlaspi caerulescens (Ganges ecotype) is a known Cd hyperaccumulator, however, the ligands which coordinate to Cd ions in the leaves have not been identified. In the present study, the chemical form of Cd was investigated by using (113)supercript stopCd-nuclear magnetic resonance (NMR) spectroscopy. Plants were grown hydroponically with a highly enriched (113)supercript stopCd stable isotope. Measurements of (113)supercript stopCd-NMR with intact leaves showed a signal at the chemical shift of around -16 ppm. Crude leaf sap also gave a similar chemical shift. Purification by gel filtration (Sephadex G-10), followed by cationic and anionic exchange chromatography, showed that Cd occurred only in the anionic fraction, which gave the same chemical shift as intact leaves. Further purification of the anionic fraction, combined with (113)supercript stopCd- and (1)supercript stopH-NMR studies, revealed that only the fraction containing malate showed a chemical shift similar to the intact leaves. These results indicate that Cd was coordinated mainly with malate in the leaves of T. caerulescens. The malate concentration in the leaves was not affected by increasing Cd concentration in the solution, suggesting that malate synthesis is not induced by Cd. Because the Cd-malate complex is relatively weak, we suggest that the complex forms inside the vacuoles as a result of an efficient tonoplast transport of Cd and a constitutively high concentration of malate in the vacuoles, and that the formation of the Cd-malate complex may lead to a decrease of subsequent Cd efflux to the cytoplasm.

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  • Isolation and characterization of a rice mutant hypersensitive to Al

    JF Ma, S Nagao, CF Huang, M Nishimura

    PLANT AND CELL PHYSIOLOGY46 ( 7 ) 1054 - 1061   2005年7月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Rice (Oryza sativa L.) is a highly Al-resistant species among small grain crops, but the mechanism responsible for the high Al resistance has not been elucidated. In this study, rice mutants sensitive to Al were isolated from M-3 lines derived from an Al-resistant cultivar, Koshihikari, irradiated with gamma-rays. Relative root elongation was used as a parameter for evaluating Al resistance. After initial screening plus two rounds of confirmatory testing, a mutant (als1) was isolated from a total of 560 lines. This mutant showed a phenotype similar to the wild-type plant in the absence of Al. However, in the presence of 10 mu M Al, root elongation was inhibited 70% in the mutant, but only 8% in the wild-type plant. The mutant also showed poorer root growth in acid soil. The Al content of root apices (0-1 cm) was much lower in the wild-type plant. The sensitivity to other metals including Cd and La did not differ between the mutant and the wild-type plants. A small amount of citrate was secreted from the roots of the mutant in response to Al stress, but there was no difference from that secreted by the wild-type plant. Genetic analysis of F-2 populations between als1 and wild-type plants showed that the Al-resistant seedlings and Al-sensitive seedlings segregated at a 3 : 1 ratio, indicating that the high sensitivity to Al in als1 is controlled by a single recessive gene. The gene was mapped to the long arm of chromosome 6, flanked by InDel markers MaOs0619 and MaOs0615.

    DOI: 10.1093/pcp/pci116

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  • Uptake system of silicon in different plant species

    N Mitani, JF Ma

    JOURNAL OF EXPERIMENTAL BOTANY56 ( 414 ) 1255 - 1261   2005年4月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    The accumulation of silicon (Si) in the shoots varies considerably among plant species, but the mechanism responsible for this variation is poorly understood. The uptake system of Si was investigated in terms of the radial transport from the external solution to the root cortical cells and the release of Si from the cortical cells to the xylem in rice, cucumber, and tomato, which differ greatly in shoot Si concentration. Symplasmic solutions of the root tips were extracted by centrifugation. The concentrations of Si in the root-cell symplast in all species were higher than that in the external solution, although the concentration in rice was 3- and 5-fold higher than that in cucumber and tomato, respectively. A kinetic study showed that the radial transport of Si was mediated by a transporter with a K-m value of 0.15 mM in all species, but with different V-max values in the order of rice &gt; cucumber &gt; tomato. In the presence of the metabolic inhibitor 2,4-dinitrophenol, and at low temperature, the Si concentration in the root-cell symplast decreased to a level similar to that of the apoplasmic solution. These results suggest that both transporter-mediated transport and passive diffusion of Si are involved in the radial transport of Si and that the transporter-mediated transport is an energy-dependent process. The Si concentration of xylem sap in rice was 20- and 100-fold higher than that in cucumber and tomato, respectively. In contrast to rice, the Si concentration in the xylem sap was lower than that in the external solution in cucumber and tomato. A kinetic study showed that xylem loading of Si was also mediated by a kind of transporter in rice, but by passive diffusion in cucumber and tomato. These results indicate that a higher density of transporter for radial transport and the presence of a transporter for xylem loading are responsible for the high Si accumulation in rice.

    DOI: 10.1093/jxb/eri121

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  • Subcellular localisation of Cd and Zn in the leaves of a Cd-hyperaccumulating ecotype of Thlaspi caerulescens

    JF Ma, D Ueno, FJ Zhao, SP McGrath

    PLANTA220 ( 5 ) 731 - 736   2005年3月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Thlaspi caerulescens (Ganges ecotype) is able to accumulate large concentrations of cadmium (Cd) and zinc (Zn) in the leaves without showing any toxicity, suggesting a strong internal detoxification. The distribution of Cd and Zn in the leaves was investigated in the present study. Although the Cd and Zn concentrations in the epidermal tissues were 2-fold higher than those of mesophyll tissues, 65-70% of total leaf Cd and Zn were distributed in the mesophyll tissues, suggesting that mesophyll is a major storage site of the two metals in the leaves. To examine the subcellular localisation of Cd and Zn in mesophyll tissues, protoplasts and vacuoles were isolated from plants exposed to 50 muM Cd and Zn hydroponically. Pure protoplasts and vacuoles were obtained based on light-microscopic observation and the activities of marker enzymes of cytosol and vacuoles. Of the total Cd and Zn in the mesophyll tissues, 91% and 77%, respectively, were present in the protoplast, and all Cd and 91% Zn in the protoplast were localised in the vacuoles. Furthermore, about 70% and 86% of total Cd and Zn, respectively, in the leaves were extracted in the cell sap, suggesting that most Cd and Zn in the leaves is present in soluble form. These results indicate that internal detoxification of Cd and Zn in Thlaspi caerulescens leaves is achieved by vacuolar compartmentalisation.

    DOI: 10.1007/s00425-004-1392-5

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  • Identification of the silicon form in xylem sap of rice (Oryza sativa L.)

    N Mitani, JF Ma, T Iwashita

    PLANT AND CELL PHYSIOLOGY46 ( 2 ) 279 - 283   2005年2月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Rice (Oryza sativa L.) is a typical silicon (Si)-accumulating plant, but the mechanism responsible for the translocation from the root to the shoot is poorly understood. In this study, the form of Si in xylem sap was identified by 2 Si-29-nuclear magnetic resonance (NMR) spectroscopy. In rice (cv. Oochikara) cultured in a monosilicic acid solution containing 0.5 mM Si, the Si concentration in the xylem reached 6 mM,l within 30 min. In the Si-29-NMR spectra of the xylem sap, only one signal was observed at a chemical shift of -72.6 ppm, which is consistent with that of monosilicic acid. A H-1-NMR study of xylem sap did not show any significant difference between the wild-type rice and mutant rice defective in Si uptake, and the components of the xylem sap were not affected by the Si supply. The Si concentration in the xylem sap in vitro decreased from an initial 18 mM to 2.6 mM with time. Addition of xylem sap to a solution containing 8 mM Si did not prevent the polymerization of silicic acid. All these results indicate that Si is translocated in the form of monosilicic acid through the xylem and that the concentration of monosilicic acid is high in the xylem only transiently.

    DOI: 10.1093/pcp/pci018

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  • Plant root responses to three abundant soil minerals: Silicon, aluminum and iron

    JF Ma

    CRITICAL REVIEWS IN PLANT SCIENCES24 ( 4 ) 267 - 281   2005年

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:TAYLOR & FRANCIS INC  

    Silicon (Si), aluminum (Al), and iron (Fe) are the three most abundant minerals in soil; however, their effects on plants differ because they are beneficial, toxic, and essential to plant growth, respectively. High accumulation of silicon in the shoots helps some plants to overcome a range of biotic and abiotic stresses. However, plants vary in their ability to take up Si from the soil and load it into the xylem and so the accumulation of silicon varies greatly between plant species. Aluminum toxicity is characterized by a rapid inhibition of root elongation but some species and even genotypes within species can tolerate Al toxicity better than others. While the mechanisms controlling this tolerance in most of the more resistant species are poorly understood, some plants are able to detoxify Al externally and/or internally by complexation with ligands or by pH changes in the rhizosphere. Iron is taken up from the soil by two efficient mechanisms called Strategy I and Strategy II, which operate in distinct phylogenic groups. Strategy I plants increase soil Fe solubility by releasing protons and reductants/chelators, such as organic acids and phenolics, into the rhizosphere, while Strategy II plants are characterized by the secretion of ferric chelating substances (phytosiderophores) coupled with a specific Fe3+ :chelate uptake system. In this review, the molecular mechanisms underlying root response to Si, Al, and Fe are described.

    DOI: 10.1080/07352680500196017

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  • 9-26 イネケイ酸吸収関連遺伝子Lsi1の機能解析(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)

    三谷 奈見季, 山地 直樹, 玉井 一規, 小西 左江子, 矢野 昌裕, 村田 佳子, 馬 建鋒

    日本土壌肥料学会講演要旨集51 ( 0 )   2005年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-25 新規イネケイ酸吸収関連遺伝子Lsi2の単離と解析(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)

    玉井 一規, 三谷 奈見季, 山地 直樹, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集51 ( 0 )   2005年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • Genotypic variation in Fe concentration of barley grain

    JF Ma, A Higashitani, K Sato, K Takeda

    SOIL SCIENCE AND PLANT NUTRITION50 ( 7 ) 1115 - 1117   2004年12月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    Iron deficiency in humans is the most prevalent micronutrient deficiency worldwide. To screen for high Fe cultivar, genotypic variation in Fe concentration of barley grain was investigated in two collections of barley: 274 standard varieties selected at the Barley Germplasm Center of the Research Institute for Bioresources, Okayama University (SV) and 135 varieties from the Barley Core Collection of Americas (BCCAM). The Fe concentration of barley grain showed large variation, ranging from 24.6 to 63.3 mg kg(-1) in SV, from 21.0 to 83.0 mg kg(-1) in BCCAM barleys. The Fe concentration was not affected by the key characters of barley varieties, kernel row types, and hulled or hull-less. The Fe concentration was also not related to the place of origin of the barley variety. About 90% of total Fe was localized in the grain without hull. These results provide fundamental data for breeding Fe-rich cultivars and for studying the mechanisms involved in genotypic variation in Fe concentration.

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  • Characterization of the silicon uptake system and molecular mapping of the silicon transporter gene in rice

    JF Ma, N Mitani, S Nagao, S Konishi, K Tamai, T Iwashita, M Yano

    PLANT PHYSIOLOGY136 ( 2 ) 3284 - 3289   2004年10月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa L. cv Oochikara) is a typical silicon-accumulating plant, but the mechanism responsible for the high silicon uptake by the roots is poorly understood. We characterized the silicon uptake system in rice roots by using a low-silicon rice mutant (Isi1) and wild-type rice. A kinetic study showed that the concentration of silicon in the root symplastic solution increased with increasing silicon concentrations in the external solution but saturated at a higher concentration in both lines. There were no differences in the silicon concentration of the symplastic solution between the wild-type rice and the mutant. The form of soluble silicon in the root, xylem, and leaf identified by Si-29-NMR was also the same in the two lines. However, the concentration of silicon in the xylem sap was much higher in the wild type than in the mutant. These results indicate that at least two transporters are involved in silicon transport from the external solution to the xylem and that the low-silicon rice mutant is defective in loading silicon into xylem rather than silicon uptake from external solution to cortical cells. To map the responsible gene, we performed a bulked segregant analysis by using both microsatellite and expressed sequence tag-based PCR markers. As a result, the gene was mapped to chromosome 2, flanked by microsatellite marker RM5303 and expressed sequence tag-based PCR marker E60168.

    DOI: 10.1104/pp.104.047365

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  • Cadmium and zinc accumulation by the hyperaccumulator Thlaspi caerulescens from soils enriched with insoluble metal compounds

    D Ueno, FJ Zhao, RF Shen, JF Ma

    SOIL SCIENCE AND PLANT NUTRITION50 ( 4 ) 511 - 515   2004年8月

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    記述言語:英語   出版者・発行元:JAPANESE SOC SOIL SCIENCE PLANT NUTRITION  

    Thlaspi caerulescens J. & C. Presl is a hyperaccumulator of both Zn and Cd. A pot experiment was carried out to investigate whether T caerulescens (the Ganges ecotype) could utilize Cd and Zn from insoluble sources. An uncontaminated soil was enriched with either insoluble Cd compounds (CdS or CdCO3), or soluble Cd (CdSO4) at a rate of 56 mg Cd kg(-1) soil. In a separate experiment, soil was enriched with either ZnSO4, Zn-3(PO4)(2), or ZnS at a rate of 100 mg Zn kg(-1). Neither soluble nor insoluble forms of Cd or Zn affected the growth of T caerulescens. The Cd concentration of the shoots of the plants grown on the unenriched soil was 40 mg kg(-1) dry wt., whereas the concentration in the case of the enriched soil reached 2,050 to 2,900 mg kg(-1) dry wt. However, there were no significant differences in the shoot Cd concentration between the treatments with soluble or insoluble Cd compounds, even though the Cd concentration in the soil solution was in the order of CdSO4 much greater thanCdC03 &gt; CdS. T caerulescens grown on the ZnS-enriched soil accumulated up to 6,900 mg kg(-1) Zn in the shoots, although Zn accumulation was 1.5-fold higher with the addition of the more soluble compounds Zn-3(PO4)(2) or ZnSO4. These results indicate that the Ganges ecotype of T caerulescens is able to utilize both insoluble Cd and Zn compounds.

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  • Interactions between Cd and Zn in relation to their hyperaccumulation in Thlaspi caerulescens

    D Ueno, FJ Zhao, JF Ma

    SOIL SCIENCE AND PLANT NUTRITION50 ( 4 ) 591 - 597   2004年8月

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    記述言語:英語   出版者・発行元:JAPANESE SOC SOIL SCIENCE PLANT NUTRITION  

    Ganges, an ecotype of Thlaspi caerulescens from southern France accumulates both Zn and Cd to extraordinary levels in the shoots. The interactions between Zn and Cd in this ecotype were investigated using solution culture and pot experiments with soil. Results from both relatively long-term (4 weeks) and short-term (1 week) solution culture experiments indicated that Cd accumulation in the shoots was not affected by the supply of a 4 to 10-fold excess of Zn, whereas the Cd concentration of the roots decreased with increasing Zn concentration in the solution. By contrast, the supply of an equimolar concentration of Cd significantly reduced the Zn concentration in both roots and shoots. However, when 4-fold more Zn than Cd was supplied, Cd-induced decrease in Zn accumulation was not observed. In soil treated with Cd or Zn, addition of one metal increased the metal concentration of the other in the soil solution. However, Zn accumulation by T caerulescens decreased by Cd addition, whereas Cd accumulation was not affected by Zn addition. These results suggested that the Ganges ecotype of T. caerulescens displayed different uptake systems for Cd and Zn, and that Cd competed with Zn uptake while Zn did not compete with Cd uptake.

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  • Molecular mapping of a gene responsible for Al-activated secretion of citrate in barley. 国際誌

    Jian Feng Ma, Sakiko Nagao, Kazuhiro Sato, Hiroyuki Ito, Jun Furukawa, Kazuyoshi Takeda

    Journal of experimental botany55 ( 401 ) 1335 - 41   2004年6月

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    記述言語:英語  

    Aluminium (Al) toxicity is an important limitation to barley (Hordeum vulgare L.) on acid soil. Al-resistant cultivars of barley detoxify Al externally by secreting citrate from the roots. To link the genetics and physiology of Al resistance in barley, genes controlling Al resistance and Al-activated secretion of citrate were mapped. An analysis of Al-induced root growth inhibition from 100 F2 seedlings derived from an Al-resistant cultivar (Murasakimochi) and an Al-sensitive cultivar (Morex) showed that a gene associated with Al resistance is localized on chromosome 4H, tightly linked to microsatellite marker Bmag353. Quantitative trait locus (QTL) analysis from 59 F4 seedlings derived from an F3 plant heterozygous at the region of Al resistance on chromosome 4H showed that a gene responsible for the Al-activated secretion of citrate was also tightly linked to microsatellite marker Bmag353. This QTL explained more than 50% of the phenotypic variation in citrate secretion in this population. These results indicate that the gene controlling Al resistance on barley chromosome 4H is identical to that for Al-activated secretion of citrate and that the secretion of citrate is one of the mechanisms of Al resistance in barley. The identification of the microsatellite marker associated with both Al resistance and citrate secretion provides a valuable tool for marker-assisted selection of Al-resistant lines.

    DOI: 10.1093/jxb/erh152

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  • Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses

    JF Ma

    SOIL SCIENCE AND PLANT NUTRITION50 ( 1 ) 11 - 18   2004年2月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:JAPANESE SOC SOIL SCIENCE PLANT NUTRITION  

    Although silicon (Si) has not been recognized as an essential element for plant growth, the beneficial effects of Si have been observed in a wide variety of plant species. The beneficial effects of Si are usually expressed more clearly in Si-accumulating plants under various abiotic and biotic stress conditions. Silicon is effective in controlling various pests and diseases caused by both fungi and bacteria in different plant species. Silicon also exerts alleviative effects on various abiotic stresses including salt stress, metal toxicity, drought stress, radiation damage, nutrient imbalance, high temperature, freezing and so on. These beneficial effects are mainly attributed to the high accumulation of silica on the tissue surface although other mechanisms have also been proposed. To obtain plants resistant to multiple stresses, genetic modification of the root ability to take up Si has been proposed. In this review, the role of Si in conferring resistance to mutiple stresses is described.

    DOI: 10.1080/00380768.2004.10408447

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  • Form of Al changes with Al concentration in leaves of buckwheat

    RF Shen, T Iwashita, JF Ma

    JOURNAL OF EXPERIMENTAL BOTANY55 ( 394 ) 131 - 136   2004年1月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Buckwheat (Fagopyrum esculentum Moench. cv. Jianxi) is known as an Al-accumulating plant. The process leading to the accumulation of Al in the leaves was investigated, focusing on the chemical form of Al using Al-27-nuclear magnetic resonance. Leaves with different Al concentrations were prepared by growing buckwheat on a very acidic soil (Andosol) amended with or without CaCO3 (1 or 3 g kg(-1) soil). When the Al concentration of the leaves was lower, only one major signal was observed at a chemical shift of 16.1 ppm, which was assigned to an Al-oxalate complex at a 1:3 ratio. However, when the Al concentration of the leaves increased to a high level (e.g. 12 g Al kg(-1)), an additional signal at a chemical shift of 11.2 ppm was observed. This signal was assigned to an Al-citrate complex at a 1:1 ratio. In the leaf with a high Al concentration, both Al-oxalate (1:3) and Al-citrate (1:1) were detected in marginal and middle parts, while only Al-oxalate was detected in the basal part. The oxalate concentration did not differ very much between leaves with low and high Al concentrations at the same position, while citrate concentration significantly increased with increasing Al concentration when the oxalate/Al ratio became lower than 3.0. As the Al-citrate complex has been demonstrated to be the form of transport in the xylem, the results suggest that when internal oxalate is enough to form a complex with Al at a 3:1 ratio in the leaves with a low Al concentration, Al-citrate converts to Al-oxalate. However, this conversion does not occur in the leaves with a very high Al concentration, resulting in the coexistence of both Al-oxalate and Al-citrate complexes.

    DOI: 10.1093/jxb/erh016

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  • Aluminum targets elongating cells by reducing cell wall extensibility in wheat roots

    JF Ma, RF Shen, S Nagao, E Tanimoto

    PLANT AND CELL PHYSIOLOGY45 ( 5 ) 583 - 589   2004年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Phytotoxicity of aluminum is characterized by a rapid inhibition of root elongation at micromolar concentrations, however, the mechanisms primarily responsible for this response are not well understood. We investigated the effect of Al on the viscosity and elasticity parameters of root cell wall by a creep-extension analysis in two cultivars of wheat (Triticum aestivum L.) differing in Al resistance. The root elongation and both viscous and elastic extensibility of cell wall of the root apices were hardly affected by the exposure to 10 muM Al in an Al-resistant cultivar, Atlas 66. However, similar exposure rapidly inhibited root elongation in an Al-sensitive cultivar, Scout 66 and this was associated with a time-dependent accumulation of Al in the root tissues with more than 77% residing in the cell wall. Al caused a significant decrease in both the viscous and elastic extensibility of cell wall of the root apices of Scout 66. The "break load" of the root apex of Scout 66 was also decreased by Al. However, neither the viscosity nor elasticity of the cell wall was affected by in vitro Al treatment. Furthermore, pre-treatment of seedlings with Al in conditions where root elongation was slow (i.e. low temperature) did not affect the subsequent elongation of roots in a 0 Al treatment at room temperature. These results suggest that the Al-dependent changes in the cell wall viscosity and elasticity are involved in the inhibition of root growth. Furthermore, for Al to reduce cell wall extensibility it must interact with the cell walls of actively elongating cells.

    DOI: 10.1093/pcp/pch060

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  • アポプラストと植物栄養-無機栄養を中心として- 4.アプポラストにおける金属毒性と耐性

    馬 建鋒

    日本土壌肥料学雑誌75:505-509   2004年

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  • イネ低ケイ酸含量突然変異体 (lsi1) の解析と原因遺伝子のマッピング(9. 植物の無機栄養, 2004年度大会講演要旨集)

    玉井 一規, 三谷 奈見季, 長尾 咲子, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集50 ( 0 )   2004年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • イネにおけるケイ酸輸送形態の同定(関西支部講演会, 日本土壌肥料学会支部講演会講演要旨集2004年度)

    三谷 奈見季, 岩下 孝, 馬 建鋒

    日本土壌肥料学会講演要旨集50 ( 0 )   2004年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 異なる植物におけるケイ酸吸収及び輸送機構に関する解析(9. 植物の無機栄養, 2004年度大会講演要旨集)

    三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集50 ( 0 )   2004年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • Characterization of phytosiderophore secretion under Fe deficiency stress in Festuca rubra

    JF Ma, H Ueno, D Ueno, AD Rombola, T Iwashita

    PLANT AND SOIL256 ( 1 ) 131 - 137   2003年9月

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    記述言語:英語   出版者・発行元:KLUWER ACADEMIC PUBL  

    In the present study we investigated the response to iron (Fe) deficiency in two cultivars of Festuca rubra L. (Rubina and Barnica) used in correction of chlorosis of fruit trees cultivated on calcareous soils. We found that a Fe-chelating compound, identified as 2'-deoxymugineic acid (DMA), was secreted from the roots in response to Fe-deficiency in both cultivars. The amount of DMA secreted into solution increased with the development of Fe-deficiency. The secretion showed a distinct diurnal rhythm characterized by a secretion peak at between 2 and 5 hours after sunrise at 20degreesC. However, this secretion peak was delayed by 3 hour at low temperature (&lt; 10&DEG;C) and occurred 3 h earlier at high temperature (30&DEG;C). When water used for the collection of root exudates was pre-warmed (25&DEG;C) or pre-cooled (10&DEG;C), this led to an earlier or a delayed secretion compared to control (15&DEG;C) under the same air temperature, respectively. Short-term shading treatment did not affect the secretion pattern of DMA. These results demonstrate that the secretion time of DMA from the roots is, at least partly controlled by the temperature in the root environment. Overall, these findings suggest that the ability of Festuca rubra to prevent Fe chlorosis symptoms('re-greening effect') of associated fruit trees is partially related to the secretion of DMA which increase Fe availability in calcareous soils.

    DOI: 10.1023/A:1026285813248

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  • Differential Al resistance and citrate secretion in barley (Hordeum vulgare L.). 国際誌

    Zhuqing Zhao, Jian Feng Ma, Kazuhiro Sato, Kazuyoshi Takeda

    Planta217 ( 5 ) 794 - 800   2003年9月

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    記述言語:英語  

    While barley ( Hordeum vulgare L.) is the most sensitive species to Al toxicity among small-grain crops, variation in Al resistance between cultivars does exist. We examined the mechanism responsible for differential Al resistance in 21 barley varieties. Citrate was secreted from the roots in response to Al stress. A positive correlation between citrate secretion and Al resistance [(root elongation with Al)/(root elongation without Al)] and a negative correlation between citrate secretion and Al content of root apices, were obtained, suggesting that citrate secretion from the root apices plays an important role in excluding Al and thereby detoxifying Al. The Al-induced secretion of citrate was characterized using an Al-resistant variety (Sigurdkorn) and an Al-sensitive variety (Kearney). In Sigurdkorn, Al-induced secretion of citrate occurred within 20 min, and the secretion did not increase with increasing external Al concentration. The Al-induced citrate secretion ceased at low temperature (6 degrees C) and was inhibited by anion-channel inhibitors. Internal citrate content of root apices was increased by Al exposure in Sigurdkorn, but was not affected in Kearney. The activity of citrate synthase was unaffected by Al in both Al-resistant and Al-sensitive varieties. The secretion rate of organic acid anions from barley was the lowest among wheat, rye and triticale.

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  • Recent progress in the research of external Al detoxification in higher plants: a minireview

    JF Ma, J Furukawa

    JOURNAL OF INORGANIC BIOCHEMISTRY97 ( 1 ) 46 - 51   2003年9月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    Aluminum (Al) is highly toxic to plant growth. The toxicity is characterized by rapid inhibition of root elongation. However, some plant species and cultivars have evolved some mechanisms for detoxifying Al both internally and externally. In this review, the recent progress made in the research of external detoxification of Al is described. Accumulating evidence has shown that organic acids play an important role in the detoxification of Al. Some plant species and cultivars respond to Al by secreting citrate, malate or oxalate from the roots. Recently, the anion channel of malate and citrate in the plasma membrane has been characterized and a gene encoding the malate channel has been cloned. The metabolism of organic acids seems to be poorly correlated with the Al-induced secretion of organic acid anions. A number of QTLs (quantitative trait loci) for Al resistance have been identified in rice, Arabidopsis, and other species. Transgenic plants with enhanced resistance to Al have also been reported, but introduction of multiple genes may be required to gain high Al resistance in future. (C) 2003 Published by Elsevier Inc.

    DOI: 10.1016/S0162-0134(03)00245-9

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  • Al-induced efflux of organic acid anions is poorly associated with internal organic acid metabolism in triticale roots

    JE Hayes, JF Ma

    JOURNAL OF EXPERIMENTAL BOTANY54 ( 388 ) 1753 - 1759   2003年7月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    The secretion of organic acid anions from roots has been identified as a mechanism of resistance to Al. However, the process leading to the secretion of organic acid anions is poorly understood. The effect of Al on organic acid metabolism was investigated in two lines of triticale (xTriticosecale Wittmark) differing in Al-induced secretion of malate and citrate and in Al resistance. The site of Al-induced secretion of citrate and malate from a resistant line was localized to the root apices (terminal 5 mm). The levels of citrate (root apices and mature root segments) and malate (mature segments only) in roots increased during exposure to Al, but similar changes were observed in both triticale genotypes. The in vitro activities of four enzymes involved in malate and citrate metabolism (citrate synthase, phosphoenolpyruvate carboxylase, malate dehydrogenase, and NADP-isocitrate dehydrogenase) were similar for sensitive and resistant lines in both root apices and mature root segments. The response of these enzymes to pH did not differ between tolerant and sensitive lines or in the presence and absence of Al. Moreover, cytoplasmic and vacuolar pH were not affected by exposure to Al in either line. Together, these results indicate that the Al-dependent efflux of organic acid anions from the roots of triticale is not regulated by their internal levels in the roots or by the capacity of the root cells to synthesize malate and citrate.

    DOI: 10.1093/jxb/erg188

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  • Characterization of silicon uptake by rice roots

    K Tamai, JF Ma

    NEW PHYTOLOGIST158 ( 3 ) 431 - 436   2003年6月

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING LTD  

    Rice (Oryza sativa) is a typical Si-accumulating plant and it has been suggested that it has a specific uptake system for silicic acid in the roots.
    Here, we characterized this specific system in rice roots. The ability of rice roots to take up Si was much higher than that of other gramineous species.
    A kinetic study indicated that Si uptake was mediated by a type of proteinaceous transporter; the K-m value was estimated to be 0.32 mm, suggesting that the transporter had a low affinity for silicic acid. Si uptake increased linearly with time, but pretreatment with Si did not affect the uptake of Si, suggesting that the system for Si uptake was not inducible. Mercuric chloride and phloretin, significantly inhibited Si uptake, but 4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid (DIDS) did not. Mercuric chloride and phloretin also inhibited water uptake, but to a lesser extent. Si uptake was unaffected by the presence of boric acid.
    Taken together, the data indicate that the uptake of Si by rice roots is a transporter-mediated process and this transporter contains Cys residues but not Lys residues.

    DOI: 10.1046/j.1469-8137.2003.00773.x

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  • Genotypic variation in silicon concentration of barley grain

    JF Ma, A Higashitani, K Sato, K Takeda

    PLANT AND SOIL249 ( 2 ) 383 - 387   2003年2月

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    記述言語:英語   出版者・発行元:KLUWER ACADEMIC PUBL  

    Soluble silicon (Si) in foods and drinks has been suggested to have a protective effect against neurotoxicity of Al. We investigated the genotypic variation in Si concentration of barley grain, which has many uses including in livestock feeds, malts for beer and whisky, and some foods for human consumption. Two collections of barley, grown in the same field, were subjected to analysis; 274 standard varieties selected at the Barley Germplasm Center of the Research Institute for Bioresources, Okayama University (SV), and 135 varieties from the Barley Core Collection of Americans (BCCAM). The Si concentration of barley grain showed large variation, ranging from 0 (under detection) to 3600 mg kg(-1) in SV and from 0 to 3800 mg kg(-1) in BCCAM barleys. The Si concentration was much lower in hull-less barley than in hulled barley. The Si concentration of two-row barley was similar to that of six-row barley, suggesting that Si concentration is not affected by the number of spike rows. Si concentration also did not differ with the origin of the barley variety. More than 80% of total Si was localized in the hull. The Si concentration of the hull was between 15 343 and 27 089 mg kg(-1) in the varieties tested. A close correlation was obtained between the Si concentrations of barley grains harvested in different years, suggesting that the variation in Si concentration of barley grain is controlled genetically. These results provide fundamental data for breeding Si-rich cultivars.

    DOI: 10.1023/A:1022842421926

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  • 植物による鉄の獲得と集積

    馬 建鋒

    肥料95:51-57   2003年

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  • 植物のケイ酸吸収機構

    馬 建鋒

    肥料94:26-32   2003年

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  • 9-68 異なる植物によるケイ酸吸収機構の解析(9.植物の無機栄養)

    三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集49 ( 0 )   2003年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • A rice mutant defective in Si uptake

    JF Ma, K Tamai, M Ichii, GF Wu

    PLANT PHYSIOLOGY130 ( 4 ) 2111 - 2117   2002年12月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) accumulates silicon (Si) in the tops to levels up to 10.0% of shoot dry weight, but the mechanism responsible for high Si uptake by rice roots is not understood. We isolated a rice mutant (GR1) that is defective in active Si uptake by screening M-2 seeds (64,000) of rice cv Oochikara that were treated with 10(-3) M sodium azide for 6 h at 25degreesC. There were no phenotypic differences between wild type (WT) and GR1 except that the leaf blade of GR1 remained droopy when Si was supplied. Uptake experiments showed that Si uptake by GR1 was significantly lower than that by WT at both low and high Si concentrations. However, there was no difference in the uptake of other nutrients such as phosphorus and potassium. Si concentration in the xylem sap of WT was 33-fold that of the external solution, but that of GR1 was 3-fold higher than the external solution at 0.15 mm Si. Si uptake by WT was inhibited by metabolic inhibitors including NaCN and 2,4-dinitrophenol and by low temperature, whereas Si uptake by GR1 was not inhibited by these agents. These results suggest that an active transport system for Si uptake is disrupted in GR1. Analysis of F-2 populations between GR1 and WT showed that roots with high Si uptake and roots with low Si uptake segregated at a 3:1 ratio, suggesting that GR1 is a recessive mutant of Si uptake.

    DOI: 10.1104/pp.010348

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  • Compartmentation of aluminium in leaves of an Al-accumulator, Fagopyrum esculentum Moench.

    RF Shen, JF Ma, M Kyo, T Iwashita

    PLANTA215 ( 3 ) 394 - 398   2002年7月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Buckwheat (Fagopyrum esculentum Moench.) is an Al-accumulating plant, but the internal mechanism(s) of detoxification of Al is not fully understood. We investigated the subcellular localization of Al in the leaves of this plant (cv. Jianxi) by directly isolating protoplasts and vacuoles. Pure protoplasts and vacuoles from the leaves of buckwheat, grown hydroponically in Al solution, were obtained based oil light-microscopic observation and the activities of marker enzymes of cytosol and vacuoles. More than 80% of total Al in the leaves was present in the protoplasts. and was identified as an Al-oxalate complex (1:3 ratio) by 27 Al-nuclear magnetic resonance. Oxalate and Al in the protoplasts was localized in the vacuoles. These results suggest that internal detoxification of Al in the buckwheat leaves is achieved by both complexation with oxalate and sequestration into vacuoles.

    DOI: 10.1007/s00425-002-0763-z

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  • Response of rice to Al stress and identification of quantitative trait loci for Al tolerance

    JF Ma, RF Shen, ZQ Zhao, M Wissuwa, Y Takeuchi, T Ebitani, M Yano

    PLANT AND CELL PHYSIOLOGY43 ( 6 ) 652 - 659   2002年6月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Rice (Oryza sativa L.) shows the highest tolerance to Al toxicity among small-grain cereal crops, however, the mechanisms and genetics responsible for its high Al tolerance are not yet well understood. We investigated the response of rice to Al stress using the japonica variety Koshihikari in comparison to the indica variety Kasalath. Koshihikari showed higher tolerance at various Al concentrations than Kasalath. The Al content in root apexes was less in Koshihikari than in Kasalath, suggesting that exclusion mechanisms rather than internal detoxification are acting in Koshihikari. Al-induced secretion of citrate was observed in both Koshihikari and Kasalath, however, it is unlikely to be the mechanism for Al tolerance because there was no significant difference in the amount of citrate secreted between Koshihikari and Kasalath. Quantitative trait loci (QTLs) for Al tolerance were mapped in a population of 183 backcross inbred lines (BILs) derived from a cross of Koshihikari and Kasalath. Three putative QTLs controlling Al tolerance were detected on chromosomes 1, 2 and 6. Kasalath QTL alleles on chromosome 1 and 2 reduced Al tolerance but increased tolerance on chromosome 6. The three QTLs explained about 27% of the phenotypic variation in Al tolerance. The existence of QTLs for Al tolerance was confirmed in substitution lines for corresponding chromosomal segments.

    DOI: 10.1093/pcp/pcf081

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  • Aluminum-induced secretion of both citrate and malate in rye

    XF Li, JF Ma, H Matsumoto

    PLANT AND SOIL242 ( 2 ) 235 - 243   2002年5月

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    記述言語:英語   出版者・発行元:KLUWER ACADEMIC PUBL  

    Aluminum (Al)-resistant mechanisms responsible for Al-induced secretion of organic acids are poorly understood. In this study, we characterized the Al-induced secretion of both citrate and malate from rye (Secale cereale L. cv. King). Secretion of organic acids increased with increasing concentration (10, 30 and 50 muM) and duration of Al treatments. Neither phosphorous (P) deficiency up to 15 days nor addition of 50 muM lanthanum, 50 muM lead, 10 muM cadmium, or 200 muM manganese caused secretion of organic acids, suggesting that this secretion was a specific response to Al stress. Aluminum activated citrate synthase, the main enzyme for the synthesis of citrate, but its activation occurred only in the root tip. The elongation of roots of an Al-sensitive cultivar of wheat (Tritium aestivum L. cv. Scout 66) was not inhibited by 50 muM Al in the presence of externally applied 50 muM citrate or 400 muM malate. The secretion of citrate and malate from intact rye roots exposed to 50 muM Al corresponded to 31.3 +/- 1.7 muM and 11.5 +/- 2.5 muM, respectively, in the rhizosphere based on an assumption of a 2 mm thick unstirred layer around root tips. This result indicated that Al-resistance in rye was achieved by the Al-induced synthesis of citrate in root apices followed by Al-induced specific secretion of citrate from root tips.

    DOI: 10.1023/A:1016257906153

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  • カルシウム欠乏ストレス(固体、組織、細胞レベル).

    馬 建鋒

    植物栄養・肥料の事典、朝倉書店   2002年

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  • 土壌酸性をめぐる研究の進展と今後の課題―レビューと課題の抽出

    犬伏和之, 平館俊太郎, 櫻井克年, 川東正幸, 馬 建鋒, 谷昌幸, 岡崎正則, 林健太郎, 坂本一憲, 三枝正彦

    日本土壌肥料学雑誌73:575-583   2002年

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  • Root-cap mucilage binds aluminum and accumulates organic acids in Zea mays L.

    Li, X. F, Ma, J. F, Matsumoto, H

    J. Plant Physiol. Mol. Biol.   2002年

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  • アポプラストの機能と無機元素

    阿江教治, 小林優, 桜井直樹, 白石友紀, 藤原徹, 馬建鋒, 間藤徹

    日本土壌肥料学雑誌73:827-832   2002年

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  • Role of root hairs and lateral roots in silicon uptake by rice

    JF Ma, S Goto, K Tamai, M Ichii

    PLANT PHYSIOLOGY127 ( 4 ) 1773 - 1780   2001年12月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    The rice plant (Oryza sativa L. cv Oochikara) is known to be a Si accumulator, but the mechanism responsible for the high uptake of Si by the roots is not well understood. We investigated the role of root hairs and lateral roots in the Si uptake using two mutants of rice, one defective in the formation of root hairs (RH2) and another in that of lateral roots (RM109). Uptake experiments with nutrient solution during both a short term (up to 12 h) and relatively long term (26 d) showed that there was no significant difference in Si uptake between RH2 and the wild type (WT), whereas the Si uptake of RM109 was much less than that of WT. The number of silica bodies formed on the third leaf in RH2 was similar to that in WT, but the number of silica bodies in RM109 was only 40% of that in WT, when grown in soil amended with Si under flooded conditions. There was also no difference in the shoot Si concentration between WT and RH2 when grown in soil under upland conditions. Using a multi-compartment transport box, the Si uptake at the root tip (0-1 cm, without lateral roots and root hairs) was found to be similar in WT, RH2, and RM109. However, the Si uptake in the mature zone (1-4 cm from root tip) was significantly lower in RM109 than in WT, whereas no difference was found in Si uptake between WT and RH2. All these results clearly indicate that lateral roots contribute to the Si uptake in rice plant, whereas root hairs do not. Analysis of F-2 populations between RM109 and WT showed that Si uptake was correlated with the presence of lateral roots and that the gene controlling formation of lateral roots and Si uptake is a dominant gene.

    DOI: 10.1104/pp.127.4.1773

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  • Distribution and mobility of aluminium in an Al-accumulating plant, Fagopyrum esculentum Moench.

    RF Shen, JF Ma

    JOURNAL OF EXPERIMENTAL BOTANY52 ( 361 ) 1683 - 1687   2001年8月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Buckwheat (Fagopyrum esculentum Moench. cv. Jianxi) accumulates high concentrations of Al in the leaves without showing any toxicity. To understand the accumulation mechanism of All in buckwheat, the distribution and mobility of Al in buckwheat were investigated. Relatively long-term treatment (28 d) with Al led to a decrease in Al concentration from old to young leaves, while a short-term (1 d) exposure to Al resulted in a uniform distribution of All in the leaves. When the fourth leaf was wrapped inside a transparent plastic bag to suppress transpiration, the Al concentration of this leaf was only one-quarter of that in the corresponding leaf without wrappng. Wit in a leaf, the Al concentration at the margins was much higher than that in the centre. These results indicate that Al distribution in the leaves is controlled by both rate and duration of transpiration. The mobility of Al between old and new leaves was studied by first growing plants in a solution with Al, followed by culture in a solution without Al. The Al content in the two new leaves appeared after removal of external Al was very low, whereas that in the old leaves did not decrease but continued to increase. The increased Al content was found to be translocated from Al remaining in the roots. It is concluded that Al is not mobile once it is accumulated in the leaf.

    DOI: 10.1093/jexbot/52.361.1683

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  • Aluminium tolerance in plants and the complexing role of organic acids

    JF Ma, PR Ryan, E Delhaize

    TRENDS IN PLANT SCIENCE6 ( 6 ) 273 - 278   2001年6月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:ELSEVIER SCIENCE LONDON  

    The aluminium cation Al3+ is toxic to many plants at micromolar concentrations. A range of plant species has evolved mechanisms that enable them to grow on acid soils where toxic concentrations of Al3+ can limit plant growth, Organ ic acids play a central role in these aluminium tolerance mechanisms. Some plants detoxify aluminium in the rhizosphere by releasing organic acids that chelate aluminium. In at least two species, wheat and maize, the transport of organic acid anions out of the root cells is mediated by aluminium-activated anion channels in the plasma membrane. Other plants, including species that accumulate aluminium in their leaves, detoxify aluminium internally by forming complexes with organic acids.

    DOI: 10.1016/S1360-1385(01)01961-6

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  • Regulatory mechanisms of Al-induced secretion of organic acids anions-Involvement of ABA in the Al-induced secretion of oxalate in buckwheat

    Ma, J. F, Zhang, W, Zhao, Z

    Plant Nutrition-Food security and sustainability of agro-ecosystems through basic and applied Research.   2001年

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  • Chapter 2 Silicon as a beneficial element for crop plants

    J. F. Ma, Y. Miyake, E. Takahashi

    Studies in Plant Science8 ( C ) 17 - 39   2001年

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    記述言語:英語  

    Silicon (Si) has not been proven to be an essential element for higher plants, but its beneficial effects on growth have been reported in a wide variety of crops, including rice, wheat, barley, and cucumber. Si fertilizer is applied to crops in several countries for increased productivity and sustainable production. Plants take up Si in the form of silicic acid, which is transported to the shoot, and after loss of water, it is polymerized as silica gel on the surface of leaves and stems. Evidence is lacking concerning the physiological role of Si in plant metabolism. Since the beneficial effects of this element are apt to be observed in plants which accumulate Si, the silica gel deposited on the plant surface is thought to contribute to the beneficial effects of Si, which may be small under optimized growth conditions, but become obvious under stress conditions. In this review, the effects of Si under biotic stresses (disease and insect damage) and abiotic stresses including climate stresses (typhoon and cool summer damage), water deficiency stress, and mineral stresses (deficiency of P and excess of P, Na, Mn, N and Al) are discussed. © 2001 Elsevier B.V. All rights reserved.

    DOI: 10.1016/S0928-3420(01)80006-9

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  • Pattern of aluminum-induced secretion of organic acids differs between rye and wheat

    XF Li, JF Ma, H Matsumoto

    PLANT PHYSIOLOGY123 ( 4 ) 1537 - 1543   2000年8月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT PHYSIOLOGISTS  

    Al-Induced secretion of organic acids from the roots has been considered as a mechanism of Al tolerance, but the processes leading to the secretion of organic acids are still unknown. In this study, the secretion pattern and alteration in the metabolism of organic acids under Al stress were examined in rye (Secale cereale L. cv King) and wheat (Triticum aestivum L. cv Atlas 66). Al induced rapid secretion of malate in the wheat, but a lag (6 and 10 h for melic and citric acids, respectively) between the exposure to Al and the secretion of organic acids was observed in the rye. The activities of isocitrate dehydrogenase, phosphoenolpyruvate carboxylase, and malate dehydrogenase were not affected by Al in either plant. The activity of citrate synthase was increased by the exposure to Al in the rye, but not in the wheat. The secretion of malate was not suppressed at low temperature in the wheat, but that of citrate was stopped in the rye. The Al-induced secretion of citrate from roots of the rye was inhibited by the inhibitors of a citrate carrier, which transports citrate from the mitochondria to the cytoplasm. All of these results suggest that alteration in the metabolism of organic acids is involved in the Al-induced secretion of organic acids in rye, but only activation of an anion channel seems to be responsible for the rapid secretion of malate in the wheat.

    DOI: 10.1104/pp.123.4.1537

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  • Role of organic acids in detoxification of aluminum in higher plants

    JF Ma

    PLANT AND CELL PHYSIOLOGY41 ( 4 ) 383 - 390   2000年4月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:OXFORD UNIV PRESS  

    Phytotoxicity of aluminum ion (Al3+) is a serious problem limiting crop production on acid soils. Organic acids with Al-chelating ability play an important role in the detoxification of Al both externally and internally. Al is detoxified externally by the secretion of organic acids such as citric, oxalic, and /or malic acids from the roots. The secretion of organic acids is highly specific to Al and the site of secretion is localized to the root apex. The kind of organic acids secreted as well as secretion pattern differ among plant species. There are two patterns of Al-induced secretion of organic acids: In pattern I, there is no discernible delay between the addition of Al and the onset of the release of organic acids. Activation of the anion channel seems to be involved in this pattern; In pattern II, there is a marked lag phase between the addition of Al and the onset of organic acid release. The action of genes related to the metabolism and secretion of organic acids seems to be involved in this pattern. Internal detoxification of Al in Al-accumulating plants is achieved by the formation of Al-organic acid complex. For instance, the complex of Al-citrate (1:1) in hydrangea and Al-oxalate (1:3) in buckwheat has been identified.

    DOI: 10.1093/pcp/41.4.383

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  • Aluminum tolerance genes on the short arm of chromosome 3R are linked to organic acid release in triticale

    JF Ma, S Taketa, ZM Yang

    PLANT PHYSIOLOGY122 ( 3 ) 687 - 694   2000年3月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT PHYSIOLOGISTS  

    Triticale, a hybrid between wheat and rye, shows a high degree of Al tolerance that is inherited from rye, but the mechanisms of high Al tolerance in both rye and triticale are unknown. We found that the short arm of chromosome 3R carries genes necessary for Al tolerance in triticale (x Triticosecale Wittmark cv Currency). Detailed comparative studies with a 3DS.3RL translocation line (ST22) and a non-substitution line (ST2) were conducted. Root elongation was similarly inhibited by Al in ST2 and ST22 during the first 12 h of Al treatment, but more strongly in ST22 than in ST2 at 18 h and thereafter. The root inhibition induced by other metals (Cu, Cd, and La) was similar between ST2 and ST22, suggesting that the action of the genes for Al tolerance on the short arm of triticale chromosome 3R is highly specific to Al. A 2-fold larger amount of malate and citrate was released from the roots of STZ than From ST22 at 12 and 18 h after Al treatment, respectively. The marked lag phase in the inhibition of root elongation and the release of organic acids implies that the expression of genes on the short arm of triticale chromosome 38 is induced by Al, and that these genes are necessary for the release of organic acids.

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  • Aluminum tolerance genes on the short arm of chromosome 3R are linked to organic acid release in triticale

    JF Ma, S Taketa, ZM Yang

    PLANT PHYSIOLOGY122 ( 3 ) 687 - 694   2000年3月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT PHYSIOLOGISTS  

    Triticale, a hybrid between wheat and rye, shows a high degree of Al tolerance that is inherited from rye, but the mechanisms of high Al tolerance in both rye and triticale are unknown. We found that the short arm of chromosome 3R carries genes necessary for Al tolerance in triticale (x Triticosecale Wittmark cv Currency). Detailed comparative studies with a 3DS.3RL translocation line (ST22) and a non-substitution line (ST2) were conducted. Root elongation was similarly inhibited by Al in ST2 and ST22 during the first 12 h of Al treatment, but more strongly in ST22 than in ST2 at 18 h and thereafter. The root inhibition induced by other metals (Cu, Cd, and La) was similar between ST2 and ST22, suggesting that the action of the genes for Al tolerance on the short arm of triticale chromosome 3R is highly specific to Al. A 2-fold larger amount of malate and citrate was released from the roots of STZ than From ST22 at 12 and 18 h after Al treatment, respectively. The marked lag phase in the inhibition of root elongation and the release of organic acids implies that the expression of genes on the short arm of triticale chromosome 38 is induced by Al, and that these genes are necessary for the release of organic acids.

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  • Mucilage strongly binds aluminum but does not prevent roots from aluminum injury in Zea mays

    XF Li, JF Ma, S Hiradate, H Matsumoto

    PHYSIOLOGIA PLANTARUM108 ( 2 ) 152 - 160   2000年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    The possible role of mucilage in protecting roots from aluminum (Al) injury mas investigated in Zea mays L. (cv. Golden Cross Bantam), focusing on binding of Al with mucilage and effects of mucilage on Al toxicity. Al mas bound to mucilage after the treatment of roots with 10-50 mu M Al for 1 h and 30 mu M Al for 30, 60, 90 and 120 min. Using molecular sieve chromatography (Sephadex G-100), Al was co-eluted with a high molecular mass sugar and a low molecular mass sugar. The difficulty in desorbing Al from mucilage with organic acids confirmed the strong binding strength of Al by mucilage revealed by (27)Al nuclear magnetic resonance (NMR). Al could not be desorbed completely by succinic, melic, oxalic and citric acid at a molar ratio of 1:1. It could only be completely removed by oxalic acid at a molar ratio of 20:1 (oxalate:Al). Bioassay experiments showed that cell viability and callose formation were unaffected by Al bound to mucilage. However, mucilage deprived roots had only 0.21-0.59 nmol apex(-1) higher Al content than control roots after treatment with 30 mu M Al for 1, 1.5 and 2 h. Moreover, inhibition of root elongation by 5 mu M Al for 6, 12, 24 and 36 h was independent of the presence or absence of mucilage prior to the Al treatment. These results indicate that although mucilage affects the accumulation of Al by roots, it does not confer Al resistance to Z. mays root apices.

    DOI: 10.1034/j.1399-3054.2000.108002152.x

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  • Mucilage strongly binds aluminum but does not prevent roots from aluminum injury in Zea mays

    XF Li, JF Ma, S Hiradate, H Matsumoto

    PHYSIOLOGIA PLANTARUM108 ( 2 ) 152 - 160   2000年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    The possible role of mucilage in protecting roots from aluminum (Al) injury mas investigated in Zea mays L. (cv. Golden Cross Bantam), focusing on binding of Al with mucilage and effects of mucilage on Al toxicity. Al mas bound to mucilage after the treatment of roots with 10-50 mu M Al for 1 h and 30 mu M Al for 30, 60, 90 and 120 min. Using molecular sieve chromatography (Sephadex G-100), Al was co-eluted with a high molecular mass sugar and a low molecular mass sugar. The difficulty in desorbing Al from mucilage with organic acids confirmed the strong binding strength of Al by mucilage revealed by (27)Al nuclear magnetic resonance (NMR). Al could not be desorbed completely by succinic, melic, oxalic and citric acid at a molar ratio of 1:1. It could only be completely removed by oxalic acid at a molar ratio of 20:1 (oxalate:Al). Bioassay experiments showed that cell viability and callose formation were unaffected by Al bound to mucilage. However, mucilage deprived roots had only 0.21-0.59 nmol apex(-1) higher Al content than control roots after treatment with 30 mu M Al for 1, 1.5 and 2 h. Moreover, inhibition of root elongation by 5 mu M Al for 6, 12, 24 and 36 h was independent of the presence or absence of mucilage prior to the Al treatment. These results indicate that although mucilage affects the accumulation of Al by roots, it does not confer Al resistance to Z. mays root apices.

    DOI: 10.1034/j.1399-3054.2000.108002152.x

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  • Form of aluminium for uptake and translocation in buckwheat.

    Ma, J. F, Hiradate, S

    Planta   2000年

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  • Form of aluminium for uptake and translocation in buckwheat.

    Planta   2000年

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  • 有用元素

    馬 建鋒

    農学大辞典   2000年

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  • Role of organic acids in detoxification of aluminum in higher plants.

    Plant Cell Physiol.41   384 - 390   2000年

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  • Silicon as a Beneficial Element for Crop Plants.

    In Silicon in Agriculture. Elsevier Science   2000年

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  • Al binding in the epidermis cell wall inhibits cell elongation of okra hypocotyl

    JF Ma, R Yamamoto, DJ Nevins, H Matsumoto, PH Brown

    PLANT AND CELL PHYSIOLOGY40 ( 5 ) 549 - 556   1999年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Al inhibits root elongation at micromolar concentrations, but the mechanisms leading to this process are unknown. In these studies, Al-induced inhibition of cell elongation was examined using hypocotyl of okra (Abelmoschus esculentus Moench cv, Clemson Spineless) as an experimental model. One-h exposure to Al (0.5 mM AlCl(3)) in the presence of 10 mu M auxin in 0.5 mM CaCl(2), pH 4.0 significantly inhibited auxin-induced cell elongation of okra hypocotyl segments. Elongation was further suppressed with increasing Al concentrations up to 1 mM, Treatment of the hypocotyl with 1 mM citrate for 10 minutes after 2-h exposure to Al resulted in significant recovery of elongation. The amount of Al in the cell wall relative to the total in the tissue was 96.0, 96.2, and 85.4%, respectively, following 1-, 2-, and 3-h exposure to the Al solution. The total and cell wall Al content was decreased by half after the citrate desorption treatment. Furthermore, 95% of Al was found in the epidermis, and 95% of the Al in the epidermis was associated with the cell wall. Experiments using split hypocotyl segments showed that Al exposure increased the outward bending of hypocotyl segments, suggesting that the epidermis elongation was specifically inhibited by Al. Al inhibited the autolysis of epidermis by about 20%, but had little effect on the autolysis of core tissue. Taken together, these results suggest that Al binding in the epidermal cell wall inhibits critical components in cell wall loosening mechanism, resulting in inhibition of cell elongation.

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  • Al binding in the epidermis cell wall inhibits cell elongation of okra hypocotyl

    JF Ma, R Yamamoto, DJ Nevins, H Matsumoto, PH Brown

    PLANT AND CELL PHYSIOLOGY40 ( 5 ) 549 - 556   1999年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Al inhibits root elongation at micromolar concentrations, but the mechanisms leading to this process are unknown. In these studies, Al-induced inhibition of cell elongation was examined using hypocotyl of okra (Abelmoschus esculentus Moench cv, Clemson Spineless) as an experimental model. One-h exposure to Al (0.5 mM AlCl(3)) in the presence of 10 mu M auxin in 0.5 mM CaCl(2), pH 4.0 significantly inhibited auxin-induced cell elongation of okra hypocotyl segments. Elongation was further suppressed with increasing Al concentrations up to 1 mM, Treatment of the hypocotyl with 1 mM citrate for 10 minutes after 2-h exposure to Al resulted in significant recovery of elongation. The amount of Al in the cell wall relative to the total in the tissue was 96.0, 96.2, and 85.4%, respectively, following 1-, 2-, and 3-h exposure to the Al solution. The total and cell wall Al content was decreased by half after the citrate desorption treatment. Furthermore, 95% of Al was found in the epidermis, and 95% of the Al in the epidermis was associated with the cell wall. Experiments using split hypocotyl segments showed that Al exposure increased the outward bending of hypocotyl segments, suggesting that the epidermis elongation was specifically inhibited by Al. Al inhibited the autolysis of epidermis by about 20%, but had little effect on the autolysis of core tissue. Taken together, these results suggest that Al binding in the epidermal cell wall inhibits critical components in cell wall loosening mechanism, resulting in inhibition of cell elongation.

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  • Effect of Al on the phytosiderophore-mediated solubilization of Fe and uptake of Fe-phytosiderophore complex in wheat (Triticum aestivum)

    YC Chang, JF Ma, T Iwashita, H Matsumoto

    PHYSIOLOGIA PLANTARUM106 ( 1 ) 62 - 68   1999年5月

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    記述言語:英語   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    The effect of Aluminum (Al) on phytosiderophore-mediated solubilization of insoluble Fe and the uptake of phytosiderophore-Fe3+ complex was examined in wheat (Triticum aestivum L. cv. Atlas 66). Al addition did not affect the Fe solubilization by 2'-deoxymugineic acid (DMA)I although Cu addition significantly inhibited the solubilization capacity. Addition of ten times more Al than Fe to the solution of DMA-Fe3+ complex did not decrease the absorption of the DMA-Fe3+ complex at 375 nm. Furthermore, NMR study indicated that Al did not shift the proton chemical shifts of DMA, All these results suggest that Al could not form a complex with the phytosiderophore, and is thereby unlikely to affect the profess of phytosiderophore-mediated solubilization of Fe, Exposure of root to Al up to 100 mu M for 3 h did not inhibit the DMA-Fe3+ uptake by the roots, but longer pretreatment ( &gt; 6 h) inhibited the uptake of the DMA-Fe3+ by more than 50%, Neither the uptake of DNA-Fe3+ nor root elongation was inhibited by 24 h pretreatment with 10 mu M Al, but both uptake and root elongation were inhibited by higher Al (&gt; 20 mu M) pretreatment, These results suggest that Al did nor directly block the transport of the phytosiderophore-Fe3+ complex, and that the decreased uptake of the phytosiderophore-Fe3+ complex resulted from the roots being damaged by Al.

    DOI: 10.1034/j.1399-3054.1999.106109.x

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  • Effect of Al on the phytosiderophore-mediated solubilization of Fe and uptake of Fe-phytosiderophore complex in wheat (Triticum aestivum)

    YC Chang, JF Ma, T Iwashita, H Matsumoto

    PHYSIOLOGIA PLANTARUM106 ( 1 ) 62 - 68   1999年5月

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    記述言語:英語   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    The effect of Aluminum (Al) on phytosiderophore-mediated solubilization of insoluble Fe and the uptake of phytosiderophore-Fe3+ complex was examined in wheat (Triticum aestivum L. cv. Atlas 66). Al addition did not affect the Fe solubilization by 2'-deoxymugineic acid (DMA)I although Cu addition significantly inhibited the solubilization capacity. Addition of ten times more Al than Fe to the solution of DMA-Fe3+ complex did not decrease the absorption of the DMA-Fe3+ complex at 375 nm. Furthermore, NMR study indicated that Al did not shift the proton chemical shifts of DMA, All these results suggest that Al could not form a complex with the phytosiderophore, and is thereby unlikely to affect the profess of phytosiderophore-mediated solubilization of Fe, Exposure of root to Al up to 100 mu M for 3 h did not inhibit the DMA-Fe3+ uptake by the roots, but longer pretreatment ( &gt; 6 h) inhibited the uptake of the DMA-Fe3+ by more than 50%, Neither the uptake of DNA-Fe3+ nor root elongation was inhibited by 24 h pretreatment with 10 mu M Al, but both uptake and root elongation were inhibited by higher Al (&gt; 20 mu M) pretreatment, These results suggest that Al did nor directly block the transport of the phytosiderophore-Fe3+ complex, and that the decreased uptake of the phytosiderophore-Fe3+ complex resulted from the roots being damaged by Al.

    DOI: 10.1034/j.1399-3054.1999.106109.x

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  • Biosynthesis of phytosiderophores in several Triticeae species with different genomes

    JF Ma, S Taketa, YC Chang, K Takeda, H Matsumoto

    JOURNAL OF EXPERIMENTAL BOTANY50 ( 334 ) 723 - 726   1999年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    To examine variation in phytosiderophore biosynthesis in Triticeae, phytosiderophores were investigated in wild and cultivated species of wheat and barley with different genomes. All wheats tested including hexaploid (AABBDD), tetraploid (AABB), and diploid (AA or Un) lines produced only one phytosiderophore, 2'-deoxymugineic acid. The phytosiderophores biosynthesized in wild barleys varied among species. Using substitution-type triticale lines and wheat-barley addition lines, it was revealed that, in triticale, genes for the biosynthesis of both mugineic and hydroxymugineic acids were located in the long arm of chromosome 5R and that, in barley, the gene for production of mugineic acid was located in the long arm of chromosome 4H.

    DOI: 10.1093/jexbot/50.334.723

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  • Biosynthesis of phytosiderophores in several Triticeae species with different genomes

    JF Ma, S Taketa, YC Chang, K Takeda, H Matsumoto

    JOURNAL OF EXPERIMENTAL BOTANY50 ( 334 ) 723 - 726   1999年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    To examine variation in phytosiderophore biosynthesis in Triticeae, phytosiderophores were investigated in wild and cultivated species of wheat and barley with different genomes. All wheats tested including hexaploid (AABBDD), tetraploid (AABB), and diploid (AA or Un) lines produced only one phytosiderophore, 2'-deoxymugineic acid. The phytosiderophores biosynthesized in wild barleys varied among species. Using substitution-type triticale lines and wheat-barley addition lines, it was revealed that, in triticale, genes for the biosynthesis of both mugineic and hydroxymugineic acids were located in the long arm of chromosome 5R and that, in barley, the gene for production of mugineic acid was located in the long arm of chromosome 4H.

    DOI: 10.1093/jexbot/50.334.723

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  • Genes controlling hydroxylations of phytosiderophores are located on different chromosomes in barley (Hordeum vulgare L.)

    JF Ma, S Taketa, YC Chang, T Iwashita, H Matsumoto, K Takeda, K Nomoto

    PLANTA207 ( 4 ) 590 - 596   1999年2月

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    記述言語:英語   出版者・発行元:SPRINGER VERLAG  

    Phytosiderophores, mugineic acids, have been demonstrated to be involved in Fe acquisition in gramineous plants. In this study, chromosomal arm locations of genes encoding for biosynthesis of various phytosiderophores were identified in a cultivar of barley (Hordeum vulgare L. cv. Betzes). Using wheat (Triticum aestivum L. cv, Chinese Spring)-barley (cv. Betzes) ditelosomic addition lines for 4HS and 4HL, a gene for hydroxylation of 2'-deoxymugineic acid to mugineic acid was localized to the long arm of barley chromosome 4H. To locate the gene for hydroxylation of mugineic acid to 3-epihydroxymugineic acid, hybrids between the 4H addition line and other wheat-barley addition lines were studied, Only a hybrid between 4H and 7H addition lines produced 3-epihydroxymugineic acid. The gene was further localized to the long arm of chromosome 7H by feeding mugineic acid to ditelosomic addition lines for 7HS and 7HL. A new phytosiderophore was discovered in both 7H and 7HL addition lines, which was identified to be 3-epihydroxy-2'-deoxymugineic acid by detailed nuclear magnetic resonance studies. These results revealed that in barley there are two pathways from 2'-deoxymugineic acid to 3-epihydroxymugineic acid: 2'-deoxymugineic acid --&gt; mugineic acid --&gt; 3-epihydroxymugineic acid and 2'-deoxymugineic acid --&gt; 3-epihydroxy-2'-deoxymugineic acid --&gt; 3-epihydroxymugineic acid. Barley genes encoding for the hydroxylations of phytosiderophores are located in different chromosomes and each gene hydroxylates different C-positions: the long arm of chromosome 4H carries the gene for hydroxylating the C-2' position and the long arm of chromosome 7H carries the gene for hydroxylating the C-3 position of the azetidine ring.

    DOI: 10.1007/s004250050522

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  • Genes controlling hydroxylations of phytosiderophores are located on different chromosomes in barley (Hordeum vulgare L.)

    JF Ma, S Taketa, YC Chang, T Iwashita, H Matsumoto, K Takeda, K Nomoto

    PLANTA207 ( 4 ) 590 - 596   1999年2月

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    記述言語:英語   出版者・発行元:SPRINGER VERLAG  

    Phytosiderophores, mugineic acids, have been demonstrated to be involved in Fe acquisition in gramineous plants. In this study, chromosomal arm locations of genes encoding for biosynthesis of various phytosiderophores were identified in a cultivar of barley (Hordeum vulgare L. cv. Betzes). Using wheat (Triticum aestivum L. cv, Chinese Spring)-barley (cv. Betzes) ditelosomic addition lines for 4HS and 4HL, a gene for hydroxylation of 2'-deoxymugineic acid to mugineic acid was localized to the long arm of barley chromosome 4H. To locate the gene for hydroxylation of mugineic acid to 3-epihydroxymugineic acid, hybrids between the 4H addition line and other wheat-barley addition lines were studied, Only a hybrid between 4H and 7H addition lines produced 3-epihydroxymugineic acid. The gene was further localized to the long arm of chromosome 7H by feeding mugineic acid to ditelosomic addition lines for 7HS and 7HL. A new phytosiderophore was discovered in both 7H and 7HL addition lines, which was identified to be 3-epihydroxy-2'-deoxymugineic acid by detailed nuclear magnetic resonance studies. These results revealed that in barley there are two pathways from 2'-deoxymugineic acid to 3-epihydroxymugineic acid: 2'-deoxymugineic acid --&gt; mugineic acid --&gt; 3-epihydroxymugineic acid and 2'-deoxymugineic acid --&gt; 3-epihydroxy-2'-deoxymugineic acid --&gt; 3-epihydroxymugineic acid. Barley genes encoding for the hydroxylations of phytosiderophores are located in different chromosomes and each gene hydroxylates different C-positions: the long arm of chromosome 4H carries the gene for hydroxylating the C-2' position and the long arm of chromosome 7H carries the gene for hydroxylating the C-3 position of the azetidine ring.

    DOI: 10.1007/s004250050522

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  • High aluminum resistance in buckwheat - I. Al-induced specific secretion of oxalic acid from root tips

    SJ Zheng, JF Ma, H Matsumoto

    PLANT PHYSIOLOGY117 ( 3 ) 745 - 751   1998年7月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    High Al resistance in buckwheat (Fagopyrum esculentum Moench. cv Jianxi) has been suggested to be associated with both internal and external detoxification mechanisms. In this study the characteristics of the external detoxification mechanism, Al-induced secretion of oxalic acid, were investigated. Eleven days of P depletion failed to induce secretion of oxalic acid. Exposure to 50 mu M LaCl3 also did not induce the secretion of oxalic acid, suggesting that this secretion is a specific response to Al stress. Secretion of oxalic acid was maintained for 8 h by a 3-h pulse treatment with 150 mu M Al. A nondestructive method was developed to determine the site of the secretion along the root. Oxalic acid was found to be secreted in the region 0 to 10 mm from the root tip. Experiments using exci