Faculty Profiles - YAMAMOTO Hideki

Papers - YAMAMOTO Hideki

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A New Method for Intraluminal Gastrointestinal Stromal Tumor Resection Using Laparoscopic Seromuscular Dissection Technique

Kazuaki Tanabe, Yuji Urabe, Noriaki Tokumoto, Takahisa Suzuki, Hideki Yamamoto, Shiro Oka, Shinji Tanaka, Hideki Ohdan

Digestive Surgery 27 ( 6 ) 461 - 465 2010

Laminin gamma 2 Mediates Wnt5a-Induced Invasion of Gastric Cancer Cells Reviewed

Hideki Yamamoto, Yasuhiko Kitadai, Hideki Yamamoto, Naohide Oue, Hideki Ohdan, Wataru Yasui, Akira Kikuchi

GASTROENTEROLOGY 137 ( 1 ) 242 - 252 2009.7

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：W B SAUNDERS CO-ELSEVIER INC

BACKGROUND & AIMS: Wnt5a expression stimulates in vitro migration and invasion of cultured gastric cancer cells by an unknown mechanism and is also correlated with aggressiveness of gastric tumors. The aim of this study was to show that Wnt5a is involved in metastasis of gastric cancer cells in vivo and to explore the molecular mechanism by which Wnt5a regulates migration and invasion. METHODS: In an experimental liver metastasis assay, Wnt5a-knockdown gastric cancer cells were injected into the spleens of nude mice. Microarray anatyses were used to compare expression patterns between mouse fibroblast L cells that stably express wild-type and a inurant form of Wnt5a to investigate Wnt5a-dependent gene expression. The expression of genes found to be regulated by Writ5a was investigated in cultured gastric cancer cells. Immunohistochemical analyses were performed to measure levels of Wnt-regulated gene products in 153 gastric cancer samples. RESULTS: Knockdown of Wnt5a in gastric cancer cells reduced the number of liver metastases that formed in nude mice. Microarray analyses indicated that Wnt5a activity induced expression of the gene encoding laminin gamma 2, a subunit of the epithelial basement membrane protein laminin-5. Wnt5a induced the expression of laminin gamma 2 through the activation of protein kinase C and c-Jun-N-terminal kinase. The invasive activity of gastric cancer cells depended on laminin gamma 2; Wnt5a expression levels correlated with those of laminin gamma 2 in diffuse-scattered type gastric tumor samples from patients. CONCLUSIONS: Wnt5a contributes to gastric cancer progression by increasing metastatic potential. Wnt5a up-regulates laminin gamma 2 to mediate gastric cancer cell aggressiveness.

File： Hideki Yamamoto; Wnt5a & GCa (Gastroenterology).pdf

DOI： 10.1053/j.gastro.2009.02.003

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Wnt3a and Dkk1 regulate distinct internalization pathways of LRP6 to tune the activation of beta-catenin signaling Reviewed

Hideki Yamamoto, Hiroshi Sakane, Hideki Yamamoto, Tatsuo Michiue, Akira Kikuchi

DEVELOPMENTAL CELL 15 ( 1 ) 37 - 48 2008.7

Language：English Publishing type：Research paper (scientific journal) Publisher：CELL PRESS

Wnt and Dickkopf (Dkk) regulate the stabilization of beta-catenin antagonistically in the Wnt signaling pathway; however, the molecular mechanism is not clear. In this study, we found that Wnt3a. acts in parallel to induce the caveolin-dependent internalization of low-density-lipoprotein receptor-related protein 6 (LRP6), as well as the phosphorylation of LRP6 and the recruitment of Axin to LRP6 on the cell surface membrane. The phosphorylation and internalization of LRP6 occurred independently of one another, and both were necessary for the accumulation of beta-catenin. In contrast, Dkk1, which inhibits Wnt3a-dependent stabilization of beta-catenin, induced the internalization of LRP6 with clathrin. Knockdown of clathrin suppressed the Dkk1-dependent inhibition of the Wnt3a response. Furthermore, Dkk1 reduced the distribution of LRP6 in the lipid raft fraction where caveolin is associated. These results indicate that Wnt3a and Dkk1 shunt LRP6 to distinct internalization pathways in order to activate and inhibit the beta-catenin signaling, respectively.

File： Dev Cell 2008; Wnt3a & Dkk1; Yamamoto H, Sakane H, Yamamoto H et al.pdf

DOI： 10.1016/j.devcel.2008.04.015

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TS-1/Weekly CDDP併用化学放射線療法が著効した進行食道癌の１例

坂口善久, 椛島章, 山本英喜, 島袋林春, 小島康知, 山村晋史, 西崎隆, 田代英哉, 松坂俊光

31 2159 - 2162 2004

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