Updated on 2025/10/23

写真a

 
MITOH Yoshihiro
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Associate Professor
Position
Associate Professor
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Degree

  • Master of Pharmaceutical Science ( Okayama University )

  • Doctor of Philosophy ( Okayama University )

Research Interests

  • Oral Physiology

  • 口腔生理学

Research Areas

  • Life Science / Oral biological science

Education

  • Okayama University   薬学研究科  

    - 1991

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    Country: Japan

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  • Kobe Gakuin University   薬学部  

    - 1988

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    Country: Japan

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Research History

  • Okayama University   Graduate School of Medicine, Dentistry and Pharmaceutical Sciences   Associate Professor

    2024.11

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    Country:Japan

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  • - 岡山大学医歯薬学総合研究科 助教

    2004

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  • - Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2004

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Professional Memberships

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Committee Memberships

  • 日本栄養・食糧学会   会員  

    2019   

  • 日本栄養・食糧学会   会員  

    2019   

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  • International Association for Dental Research   会員  

    2005 - 2006   

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    Committee type:Academic society

    International Association for Dental Research

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  • Society for Neuroscience   会員  

    2000 - 2015   

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    Committee type:Academic society

    Society for Neuroscience

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  • 岡山歯学会   評議員  

    1997   

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    Committee type:Academic society

    岡山歯学会

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  • 日本味と匂学会   評議員  

    1994   

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    Committee type:Academic society

    日本味と匂学会

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  • 歯科基礎医学会   会員  

    1994   

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    Committee type:Academic society

    歯科基礎医学会

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  • 日本生理学会   評議員  

    1993   

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    Committee type:Academic society

    日本生理学会

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  • 日本薬学会   会員  

    1988 - 2015   

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    Committee type:Academic society

    日本薬学会

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Papers

  • Exploring the Role of Ccn3 in Type III Cell of Mice Taste Buds Reviewed

    Kuanyu Wang, Yoshihiro Mitoh, Kengo Horie, Ryusuke Yoshida

    Journal of Neurochemistry   169 ( 1 )   1 - 19   2024.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jnc.16291

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  • Salivary buffering capacity is correlated with umami but not sour taste sensitivity in healthy adult Japanese subjects Reviewed

    Aiko Hyodo, Ayaka Mikami, Kengo Horie, Yoshihiro Mitoh, Yuzo Ninomiya, Seiji Iida, Ryusuke Yoshida

    Archives of Oral Biology   165   106013 - 106013   2024.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.archoralbio.2024.106013

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  • The role of GABA in modulation of taste signaling within the taste bud Reviewed

    Ayaka Mikami, Hai Huang, Aiko Hyodo, Kengo Horie, Keiko Yasumatsu, Yuzo Ninomiya, Yoshihiro Mitoh, Seiji Iida, Ryusuke Yoshida

    Pflügers Archiv - European Journal of Physiology   2024.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    Taste buds contain 2 types of GABA-producing cells: sour-responsive Type III cells and glial-like Type I cells. The physiological role of GABA, released by Type III cells is not fully understood. Here, we investigated the role of GABA released from Type III cells using transgenic mice lacking the expression of GAD67 in taste bud cells (Gad67-cKO mice). Immunohistochemical experiments confirmed the absence of GAD67 in Type III cells of Gad67-cKO mice. Furthermore, no difference was observed in the expression and localization of cell type markers, ectonucleoside triphosphate diphosphohydrolase 2 (ENTPD2), gustducin, and carbonic anhydrase 4 (CA4) in taste buds between wild-type (WT) and Gad67-cKO mice. Short-term lick tests demonstrated that both WT and Gad67-cKO mice exhibited normal licking behaviors to each of the five basic tastants. Gustatory nerve recordings from the chorda tympani nerve demonstrated that both WT and Gad67-cKO mice similarly responded to five basic tastants when they were applied individually. However, gustatory nerve responses to sweet–sour mixtures were significantly smaller than the sum of responses to each tastant in WT mice but not in Gad67-cKO mice. In summary, elimination of GABA signalling by sour-responsive Type III taste cells eliminates the inhibitory cell–cell interactions seen with application of sour–sweet mixtures.

    DOI: 10.1007/s00424-024-03007-x

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    Other Link: https://link.springer.com/article/10.1007/s00424-024-03007-x/fulltext.html

  • Distribution of alpha‐synuclein in rat salivary glands Reviewed

    Tomiko Nishitani, Yoshihiro Mitoh, Takehiro Yajima, Daisuke Tachiya, Tomohiro Hoshika, Tomohiro Fukunaga, Yoshihiro Nishitani, Ryusuke Yoshida, Itaru Mizoguchi, Hiroyuki Ichikawa, Tadasu Sato

    The Anatomical Record   2024.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    Abstract

    Expression of alpha‐synuclein (Syn), a presynaptic neuronal protein, was immunohistochemically examined in intact rat submandibular, sublingual, and lingual glands. The submandibular gland contained abundant periductal Syn‐immunoreactive (−ir) nerve fibers. Abundant Syn‐ir varicosities were present in acini of the sublingual and serous lingual glands. By confocal laser scanning microscopy, Syn‐ir nerve fibers around smooth muscle actin (SMA)‐ir cells alone were infrequent; however, those around aquaporin‐5 (AQP5)‐ir cells alone and both SMA‐ and AQP5‐ir cells were abundant in the sublingual and serous lingual glands. SMA‐ir cells were occasionally immunoreactive for toll‐like receptor 4, a Syn receptor. Syn‐ir nerve fibers contained tyrosine hydroxylase (TH) in the submandibular gland and choline acetyltransferase (ChAT) in all examined salivary glands. In the superior cervical (SCG), submandibular, and intralingual ganglia, sympathetic and parasympathetic neurons co‐expressed Syn with TH and ChAT, respectively. SCG neurons innervating the submandibular gland contained mostly Syn. In the thoracic spinal cord, 14.7% of ChAT‐ir preganglionic sympathetic neurons co‐expressed Syn. In the superior salivatory nucleus, preganglionic parasympathetic neurons projecting to the lingual nerve co‐expressed Syn and ChAT. The present findings indicate that released Syn acts on myoepithelial cells. Syn in pre‐ and post‐ganglionic neurons may regulate neurotransmitter release and salivary volume and composition.

    DOI: 10.1002/ar.25395

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  • ラット唾液腺支配の上唾液核ニューロンに対するドーパミンの影響

    美藤 純弘, 佐藤 匡, 矢島 健大, 堀江 謙吾, 市川 博之, 吉田 竜介

    Journal of Oral Biosciences Supplement   2023   [P3 - 08]   2023.9

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    Language:Japanese   Publisher:(一社)歯科基礎医学会  

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  • Sugar signals from oral glucose transporters elicit cephalic-phase insulin release in mice Reviewed

    Mitsuhito Takamori, Yoshihiro Mitoh, Kengo Horie, Masahiko Egusa, Takuya Miyawaki, Ryusuke Yoshida

    The Journal of Physiological Sciences   73 ( 1 )   2023.7

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    Abstract

    Cephalic-phase insulin release (CPIR) occurs before blood glucose increases after a meal. Although glucose is the most plausible cue to induce CPIR, peripheral sensory systems involved are not fully elucidated. We therefore examined roles of sweet sensing by a T1R3-dependent taste receptor and sugar sensing by oral glucose transporters in the oropharyngeal region in inducing CPIR. Spontaneous oral ingestion of glucose significantly increased plasma insulin 5 min later in wild-type (C57BL/6) and T1R3-knockout mice, but intragastric infusion did not. Oral treatment of glucose transporter inhibitors phlorizin and phloretin significantly reduced CPIR after spontaneous oral ingestion. In addition, a rapid increase in plasma insulin was significantly smaller in WT mice with spontaneous oral ingestion of nonmetabolizable glucose analog than in WT mice with spontaneous oral ingestion of glucose. Taken together, the T1R3-dependent receptor is not required for CPIR, but oral glucose transporters greatly contribute to induction of CPIR by sugars.

    DOI: 10.1186/s12576-023-00875-3

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    Other Link: https://link.springer.com/article/10.1186/s12576-023-00875-3/fulltext.html

  • Taste Responses and Ingestive Behaviors to Ingredients of Fermented Milk in Mice Reviewed

    Yamase Yuko, Huang Hai, Mitoh Yoshihiro, Egusa Masahiko, Miyawaki Takuya, Yoshida Ryusuke

    Foods   12 ( 6 )   1150   2023.3

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    DOI: 10.3390/foods12061150

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  • Orexin A and B in the rat superior salivatory nucleus Reviewed

    Tadasu Sato, Takehiro Yajima, Masako Fujita, Motoi Kobashi, Hiroyuki Ichikawa, Ryusuke Yoshida, Yoshihiro Mitoh

    AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL   228   102712 - 102712   2020.11

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.autneu.2020.102712

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  • The Effects of Mutual Interaction of Orexin-A and Glucagon-Like Peptide-1 on Reflex Swallowing Induced by SLN Afferents in Rats Reviewed

    Motoi Kobashi, Yuichi Shimatani, Masako Fujita, Yoshihiro Mitoh, Ryusuke Yoshida, Ryuji Matsuo

    International Journal of Molecular Sciences   21 ( 12 )   4422 - 4422   2020.6

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    (1) Background: Our previous studies revealed that orexin-A, an appetite-increasing peptide, suppressed reflex swallowing via the commissural part of the nucleus tractus solitarius (cNTS), and that glucagon-like peptide-1 (GLP-1), an appetite-reducing peptide, also suppressed reflex swallowing via the medial nucleus of the NTS (mNTS). In this study, we examined the mutual interaction between orexin-A and GLP-1 in reflex swallowing. (2) Methods: Sprague–Dawley rats under urethane–chloralose anesthesia were used. Swallowing was induced by electrical stimulation of the superior laryngeal nerve (SLN) and was identified by the electromyographic (EMG) signals obtained from the mylohyoid muscle. (3) Results: The injection of GLP-1 (20 pmol) into the mNTS reduced the swallowing frequency and extended the latency of the first swallow. These suppressive effects of GLP-1 were not observed after the fourth ventricular administration of orexin-A. After the injection of an orexin-1 receptor antagonist (SB334867) into the cNTS, an ineffective dose of GLP-1 (6 pmol) into the mNTS suppressed reflex swallowing. Similarly, the suppressive effects of orexin-A (1 nmol) were not observed after the injection of GLP-1 (6 pmol) into the mNTS. After the administration of a GLP-1 receptor antagonist (exendin-4(5-39)), an ineffective dose of orexin-A (0.3 nmol) suppressed reflex swallowing. (4) Conclusions: The presence of reciprocal inhibitory connections between GLP-1 receptive neurons and orexin-A receptive neurons in the NTS was strongly suggested.

    DOI: 10.3390/ijms21124422

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  • Effects of bitter receptor antagonists on behavioral lick responses of mice Reviewed

    Michimasa Masamoto, Yoshihiro Mitoh, Motoi Kobashi, Noriatsu Shigemura, Ryusuke Yoshida

    Neuroscience Letters   730   135041 - 135041   2020.6

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    DOI: 10.1016/j.neulet.2020.135041

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  • Effects of cevimeline on excitability of parasympathetic preganglionic neurons in the superior salivatory nucleus of rats Reviewed

    Yoshihiro Mitoh, Hirotaka Ueda, Hiroyuki Ichikawa, Masako Fujita, Motoi Kobashi, Ryuji Matsuo

    AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL   206   1 - 7   2017.9

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    DOI: 10.1016/j.autneu.2017.05.010

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  • Central glucagon like peptide-1 inhibits reflex swallowing elicited by the superior laryngeal nerve via caudal brainstem in the rat Reviewed

    Motoi Kobashi, Satoshi Mizutani, Masako Fujita, Yoshihiro Mitoh, Yuichi Shimatani, Ryuji Matsuo

    BRAIN RESEARCH   1671   26 - 32   2017.9

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    DOI: 10.1016/j.brainres.2017.07.004

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  • Aflatoxins in Rice Artificially Contaminated with Aflatoxin-producing Aspergillus flavus under Natural Storage in Japan Reviewed

    Satoshi Sugihara, Hiroyuki Doi, Masahiko Kato, Yoshihiro Mitoh, Toshihide Tsuda, Satoru Ikeda

    ACTA MEDICA OKAYAMA   70 ( 3 )   167 - 173   2016.6

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    DOI: 10.18926/AMO/54415

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  • Identification of the effective site of GLP-1 to suppress reflex swallowing Reviewed

    Satoshi Mizutani, Motoi Kobashi, Yuichi Shimatani, Masako Fujita, Yoshihiro Mitoh, Masatoshi Mizutani, Ryuji Matsuo

    CHEMICAL SENSES   40 ( 8 )   589 - 589   2015.10

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  • Orexin-A inhibits the action of GLP-1 on the reflex swallowing Reviewed

    Kobayashi Motoi, Mizutani Satoshi, Fujita Masako, Mitoh Yoshihiro, Shimatani Yuichi, Matsuo Ryuji

    The Japanese journal of taste and smell research   22 ( 3 )   371 - 374   2015

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:The Japanese Association for the Study of Taste and Smell  

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    Other Link: http://search.jamas.or.jp/link/ui/2016233777

  • Central orexin inhibits reflex swallowing elicited by the superior laryngeal nerve via caudal brainstem in the rat Reviewed

    Motoi Kobashi, Satoshi Mizutani, Masako Fujita, Yoshihiro Mitoh, Yuichi Shimatani, Ryuji Matsuo

    PHYSIOLOGY & BEHAVIOR   130   6 - 12   2014.5

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    DOI: 10.1016/j.physbeh.2014.03.009

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  • Differential involvement of two cortical masticatory areas in submandibular salivary secretion in rats Reviewed

    Naoto Maeda, Motoi Kobashi, Yoshihiro Mitoh, Masako Fujita, Shogo Minagi, Ryuji Matsuo

    BRAIN RESEARCH   1543   200 - 208   2014.1

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    DOI: 10.1016/j.brainres.2013.11.024

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  • GLP-1中枢投与による反射性嚥下抑制を惹起する部位の同定(共著) Reviewed

    水谷諭史, 小橋基, 島谷祐一, 藤田雅子, 美藤純弘, 水谷雅年, 松尾龍二

    日本味と匂学会誌   21 ( 3 )   245 - 248   2014

  • Role of the lateral hypothalamus in submandibular salivary secretion during feeding in rats.(共著) Reviewed

    Matsuo R, Kobashi M, Mitoh Y, Fujita M

    Brain Research   1596   99 - 107   2014

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    DOI: 10.1016/j.brainres.2014.11.026.

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  • GLP-1 modulates reflex swallowing of the rat Reviewed

    Mizutani Satoshi, Kobashi Motoi, Fujita Masako, Mitoh Yoshihiro, Shimatani Yuichi, Matsuo Ryuji

    The Japanese journal of taste and smell research   20 ( 3 )   237 - 240   2013

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:The Japanese Association for the Study of Taste and Smell  

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    Other Link: http://search.jamas.or.jp/link/ui/2014158479

  • オレキシン-Aによる反射性嚥下抑制を惹起する中枢作用部位の検討.(共著) Reviewed

    水谷諭史, 小橋基, 藤田雅子, 美藤純弘, 島谷祐一, 松尾龍二

    日本味と匂学会誌   19 ( 3 )   365 - 368   2012

  • Muscarinic receptor immunoreactivity in the superior salivatory nucleus neurons innervating the salivary glands of the rat Reviewed

    Hirotaka Ueda, Yoshihiro Mitoh, Masako Fujita, Motoi Kobashi, Takashi Yamashiro, Tomosada Sugimoto, Hiroyuki Ichikawa, Ryuji Matsuo

    NEUROSCIENCE LETTERS   499 ( 1 )   42 - 46   2011.7

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    DOI: 10.1016/j.neulet.2011.05.029

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  • オレキシン中枢投与による嚥下反射の抑制.(共著) Reviewed

    水谷諭史, 小橋基, 藤田雅子, 美藤純弘, 島谷祐一, 松尾龍二

    日本味と匂学会誌   18 ( 3 )   327 - 330   2011

  • Central ghrelin inhibits reflex swallowing elicited by activation of the superior laryngeal nerve in the rat Reviewed

    Motoi Kobashi, Song-Yu Xuan, Masako Fujita, Yoshihiro Mitoh, Ryuji Matsuo

    REGULATORY PEPTIDES   160 ( 1-3 )   19 - 25   2010.2

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    DOI: 10.1016/j.regpep.2009.12.014

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  • Fourth ventricular administration of ghrelin induces relaxation of the proximal stomach in the rat Reviewed

    Motoi Kobashi, Mamoru Yanagihara, Masako Fujita, Yoshihiro Mitoh, Ryuji Matsuo

    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY   296 ( 2 )   R217 - R223   2009.2

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    DOI: 10.1152/ajpregu.00878.2007

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  • Postnatal development of inhibitory synaptic transmission to superior salivatory neurons in rats

    Yoshihiro Mitoh, Makoto Funahashi, Masako Fujita, Motoi Kobashi, Ryuji Matsuo

    Journal of Medical Investigation   56 ( 1 )   270 - 272   2009

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    DOI: 10.2152/jmi.56.270

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  • Cevimeline enhances the excitability of rat superior salivatory neurons

    Hirotaka Ueda, Yoshihiro Mitoh, Hiroyuki Ichikawa, Motoi Kobashi, Takashi Yamashiro, Ryuji Matsuo

    Journal of Medical Investigation   56 ( 1 )   267 - 269   2009

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    DOI: 10.2152/jmi.56.267

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  • Immunohistochemical study on the distribution and origin of GABAergic nerve terminals in the superior salivatory nucleus

    Ayumi Matsushima, Hiroyuki Ichikawa, Masako Fujita, Yoshihiro Mitoh, Motoi Kobashi, Takashi Yamashiro, Ryuji Matsuo

    Journal of Medical Investigation   56 ( 1 )   264 - 266   2009

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    DOI: 10.2152/jmi.56.264

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  • Development of inhibitory synaptic transmission to the superior salivatory nucleus in rats Reviewed

    Yoshihiro Mitoh, Makoto Funahashi, Akihito Fujii, Masako Fujita, Motoi Kobashi, Ryuji Matsuo

    BRAIN RESEARCH   1191   47 - 54   2008.1

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    DOI: 10.1016/j.brainres.2007.11.020

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  • Purinergic modulation of area postrema neuronal excitability in rat brain slices Reviewed

    Naoki Kodama, Makoto Funahashi, Yoshihiro Mitoh, Shogo Minagi, Ryuji Matsuo

    Brain Research   1165 ( 1 )   50 - 59   2007.8

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    DOI: 10.1016/j.brainres.2007.06.003

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  • 上喉頭神経由来の反射性嚥下におよぼす中枢グレリンの調節作用.(共著) Reviewed

    小橋基, 玄松玉, 美藤純弘, 藤田雅子, 松尾龍二

    日本味と匂学会誌   14 ( 3 )   365 - 368   2007

  • Central neuropeptide Y induces proximal stomach relaxation via Y1 receptors in the dorsal vagal complex of the rat Reviewed

    M Kobashi, Y Shimatani, K Shirota, SY Xuan, Y Mitoh, R Matsuo

    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY   290 ( 2 )   R290 - R297   2006.2

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    DOI: 10.1152/ajpregu.00423.2005

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  • Variety of morphological and electrophysiological properties of area postrema neurons in adult rat brain slices Reviewed

    M Funahashi, Y Mitoh, T Akaike, R Matsuo

    NEUROSCIENCE RESEARCH   54 ( 1 )   43 - 48   2006.1

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    DOI: 10.1016/j.neures.2005.10.005

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  • Central neuropeptide Y induces gastric relaxation via Y1 receptors in the dorsal vagal complex of the rats Reviewed

    M Kobashi, K Shirota, SY Xuan, Y Shimatani, Y Mitoh, R Matsuo

    CHEMICAL SENSES   31 ( 1 )   J4 - J4   2006.1

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  • グレリン中枢投与による胃近位部食物受け入れ機能の促進作用.(共著) Reviewed

    小橋基, 柳原衞, 玄松玉, 美藤純弘, 松尾龍二

    日本味と匂学会誌   13 ( 3 )   509 - 512   2006

  • The origin of sensory nerve fibers that innervate the submandibular salivary gland in the rat Reviewed

    M Kobashi, H Ichikawa, M Kobashi, M Funahashi, Y Mitoh, R Matsuo

    BRAIN RESEARCH   1060 ( 1-2 )   184 - 187   2005.10

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    DOI: 10.1016/j.brainres.2005.08.012

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  • ニューロペプチドY-Y1受容体を介した胃近位部の食物受け入れ応答.(共著) Reviewed

    小橋 基, 城田惠祐, 玄 松玉, 島谷祐一, 美藤純弘, 松尾龍二

    日本味と匂学会誌   12 ( 3 )   239 - 242   2005

  • Activation of presynaptic 5-HT3 receptors facilities glutamatergic synaptic inputs to area postrema neurons in rat brain slices Reviewed

    M Funahashi, Y Mitoh, R Matsuo

    METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY   26 ( 8 )   615 - 622   2004.10

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    DOI: 10.1358/mf.2004.26.8.863726

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  • Nicotinic modulation of area postrema neuronal excitability in rat brain slices Reviewed

    M Funahashi, Y Mitoh, R Matsuo

    BRAIN RESEARCH   1017 ( 1-2 )   227 - 233   2004.8

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    DOI: 10.1016/j.brainres.2004.05.028

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  • The sensitivity of hyperpolarization-activated cation current (I-h) to propofol in rat area postrerna neurons Reviewed

    M Funahashi, Y Mitoh, R Matsuo

    BRAIN RESEARCH   1015 ( 1-2 )   198 - 201   2004.7

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    DOI: 10.1016/j.brainres.2004.04.043

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  • Excitatory and inhibitory postsynaptic currents of the superior salivatory nucleus innervating the salivary glands and tongue in the rat Reviewed

    Y Mitoh, M Funahashi, M Kobashi, R Matsuo

    BRAIN RESEARCH   999 ( 1 )   62 - 72   2004.2

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    DOI: 10.1016/j.brainres.2003.11.053

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  • Role of the hyperpolarization-activated cation current (I-h) in pacemaker activity in area postrema neurons of rat brain slices Reviewed

    M Funahashi, Y Mitoh, A Kohjitani, R Matsuo

    JOURNAL OF PHYSIOLOGY-LONDON   552 ( 1 )   135 - 148   2003.10

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    DOI: 10.1113/jphysiol.2003.047191

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  • Suppression of the hyperpolarization-activated inward current contributes to the inhibitory actions of propofol on rat CA1 and CA3 pyramidal neurons Reviewed

    H Higuchi, M Funahashi, T Miyawaki, Y Mitoh, A Kohjitani, M Shimada, R Matsuo

    NEUROSCIENCE RESEARCH   45 ( 4 )   459 - 472   2003.4

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    DOI: 10.1016/S0168-0102(03)00003-8

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  • Mexiletine inhibits nonadrenergic noncholinergic lower oesophageal sphincter relaxation in rabbits Reviewed

    A Kohjitani, T Miyawaki, M Funahashi, Y Mitoh, R Matsuo, M Shimada

    EUROPEAN JOURNAL OF PHARMACOLOGY   465 ( 1-2 )   145 - 151   2003.3

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    DOI: 10.1016/S0014-2999(03)01483-3

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  • Morphological study on the superior salivary nucleus innervating the submandibular and sublingual salivary glands and tongue in rats.(共著) Reviewed

    Matsuo R, Mitoh Y, Funahashi M, Kobashi M

    Dent Japan (Tokyo)   39   34 - 36   2003

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  • Electrophysiological properties of the rat area postrema neurons displaying both the transient outward current and the hyperpolarization-activated inward current Reviewed

    M Funahashi, Y Mitoh, R Matsuo

    BRAIN RESEARCH BULLETIN   58 ( 3 )   337 - 343   2002.7

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    DOI: 10.1016/S0361-9230(02)00798-0

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  • Two distinct types of transient outward currents in area postrema neurons in rat brain slices Reviewed

    M Funahashi, Y Mitoh, R Matsuo

    BRAIN RESEARCH   942 ( 1-2 )   31 - 45   2002.6

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    DOI: 10.1016/S0006-8993(02)02651-3

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  • Effects of amiloride on gustatory neural responses to salts in the frog Reviewed

    Y Kitada, K Okuda-Akabane, Y Mitoh

    CHEMICAL SENSES   26 ( 9 )   1203 - 1210   2001.11

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  • Intravenous anesthetics inhibit nonadrenergic noncholinergic lower esophageal sphincter relaxation via nitric oxide-cyclic guanosine monophosphate pathway modulation in rabbits Reviewed

    A Kohjitani, T Miyawaki, M Funahashi, Y Mitoh, R Matsuo, M Shimada

    ANESTHESIOLOGY   95 ( 1 )   176 - 183   2001.7

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  • Role of parabrachial nucleus in submandibular salivary secretion induced by bitter taste stimulation in rats Reviewed

    R Matsuo, Y Yamauchi, M Kobashi, M Funahashi, Y Mitoh, A Adachi

    AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL   88 ( 1-2 )   61 - 73   2001.4

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    DOI: 10.1016/S1566-0702(01)00234-X

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  • Salt taste responses of the IXth nerve in Sprague-Dawley rats: Lack of sensitivity to amiloride Reviewed

    Y Kitada, Y Mitoh, DL Hill

    PHYSIOLOGY & BEHAVIOR   63 ( 5 )   945 - 949   1998.3

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    DOI: 10.1016/S0031-9384(98)00009-2

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  • Effects of a bite-raising splint on the duration of the chewing cycle and the EMG activities of masticatory muscles during chewing in freely moving rabbits Reviewed

    Y Matsuka, Y Kitada, Y Mitoh, A Adachi, A Yamashita

    JOURNAL OF ORAL REHABILITATION   25 ( 2 )   159 - 165   1998.2

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  • Enhancing effect of nickel ions on the response to magnesium ions of single fibers of the frog glossopharyngeal nerve: Competitive inhibition by calcium ions of the nickel-enhanced response to magnesium ions Reviewed

    Y Kitada, Y Mitoh

    CHEMICAL SENSES   22 ( 6 )   613 - 622   1997.12

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    DOI: 10.1093/chemse/22.6.613

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  • A quantitative study of the enhancing effect of nickel ions on the taste response to sodium ions of single fibers of the frog glossopharyngeal nerve: Competitive inhibition by calcium ions of the nickel-enhanced response to sodium ions Reviewed

    Y Kitada, Y Mitoh

    CHEMICAL SENSES   21 ( 1 )   65 - 73   1996.2

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    DOI: 10.1093/chemse/21.1.65

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  • Influence of occlusal changes on masticatory behavior in rabbits Reviewed

    Y Matsuka, Y Kitada, N Jitsunari, Y Mitoh, H Yatani, A Yamashita

    BRAIN AND ORAL FUNCTIONS   1079   339 - 342   1995

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  • DEVELOPMENT OF A SENSITIVE ENZYME-IMMUNOASSAY FOR OPC-7251 - A NOVEL ANTIMICROBIAL AGENT FOR PERCUTANEOUS APPLICATION Reviewed

    N MUTO, Y MITOH, YAMAMOTO, I

    JOURNAL OF IMMUNOASSAY   11   1 - 6   1990.11

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  • ANTISCORBUTIC ACTIVITY OF L-ASCORBIC-ACID 2-GLUCOSIDE AND ITS AVAILABILITY AS A VITAMIN-C SUPPLEMENT IN NORMAL RATS AND GUINEA-PIGS Reviewed

    YAMAMOTO, I, S SUGA, Y MITOH, M TANAKA, N MUTO

    JOURNAL OF PHARMACOBIO-DYNAMICS   13 ( 11 )   688 - 695   1990.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1248/bpb1978.13.688

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Books

  • 平成30-令和2年度科学研究費補助金(基盤研究C)成果報告書

    美藤純弘( Role: Sole author)

    日本学術振興会  2021.5 

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    Language:Japanese

  • 平成30-令和2年度科学研究費補助金(基盤研究C)成果報告書

    美藤純弘( Role: Sole author)

    日本学術振興会  2021.5 

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  • A study for role of the feeding center in submandibular and sublingual salivary secretion

    Yoshihiro Mitoh( Role: Sole author)

    Japan Society for the Promotion of Science  2016 

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  • 平成21-23年度科学研究費補助金(基盤研究C)成果報告書

    美藤純弘( Role: Sole author ,  摂食関連ペプチドによる上唾液核ニューロン活動の調節機構に関する研究)

    日本学術振興会  2011 

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  • 平成19-20年度科学研究費補助金(基盤研究C)成果報告書

    美藤純弘( Role: Sole author ,  抑制系を指標にした口腔機能の生後発育の電気生理学的解析.)

    日本学術振興会  2009 

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  • 薬剤師・MR・コメディカルのための救急医療マニュアル

    *美藤純弘 他(6.3 ハチ毒アナフィラキシー・ショックとエピペンの取り扱い.)

    エルゼピア・ジャパン,東京  2007 

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    Responsible for pages:82-88  

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  • 生物学に関する試験研究論叢(第二十二集)

    *美藤純弘、根岸友恵、阿保達彦、斉藤健司、津田誠、上村一雄、畑中唯史、稲垣賢二、岸本晃治、前川賢治、河井まりこ、森宏樹、大塚文男、(口腔機能の生後発育に関する神経生理学的研究ー抑制性ニューロンの機能を指標にした解析ー.)

    財団法人 両備てい園記念財団  2007 

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    Responsible for pages:1-8  

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  • 平成17-18年度科学研究費補助金(基盤研究C)成果報告書

    *美藤純弘( Role: Sole author ,  抑制系を指標にした口腔機能の生後発育の電気生理学的解析.)

    日本学術振興会  2007 

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  • Neurobiology of Mastication-from Molecular to Systems Approach

    Kitada Y, Okuda-Akabane K, Kawarada K, Mitoh Y( Role: Joint author ,  Effect of amiloride on the taste responses of the frog glossopharyngeal nerve to Ca,Mg and Na salts.)

    Elsevier Science B.V.  1999 

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  • Hepatic osmosensors and their role in fluid homeostasis.(共著)

    ( Role: Joint author)

    John Libbey & Company  1996 

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    Responsible for pages:ReaD移行データ日付 : 1996   Language:English

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  • Influence of occlusal changes on masticatory behavior in rabbits.(共著)

    ( Role: Joint author)

    Elsevier Science B.V.  1995 

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    Responsible for pages:339-342   Language:English

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MISC

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Presentations

  • うま味刺激により活性化される神経経路の探索

    望月晴, 堀江謙吾, 美藤純弘, 吉田⻯介

    第57回日本栄養•食糧学会 中国•四国支部大会  2024.11.17 

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    Event date: 2024.11.16 - 2024.11.17

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  • マウス酸味細胞の GABA が果たす酸味甘味相互作用について

    三上彩可, 海, 兵藤藍子, 堀江謙吾, 安松啓子, 二ノ宮裕三, 美藤純弘, 飯田征二, 吉田竜介

    第57回日本栄養•食糧学会 中国•四国支部大会  2024.11.17 

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    Event date: 2024.11.16 - 2024.11.17

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • マウスIII型味細胞の GABA が果たす細胞間コミュニケーションの可能性について

    三上彩可, 黄海, 兵藤藍子, 堀江謙吾, 安松啓子, 二ノ宮裕三, 美藤純弘, 飯田征二, 吉田竜介

    第76回日本生理学会中国四国地方会  2024.11.4 

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    Event date: 2024.11.3 - 2024.11.4

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • SNAP25はType III味細胞の神経伝達と細胞維持を制御する

    堀江謙吾, 美藤純弘, 吉田竜介

    第66回歯科基礎医学会学術大会  2024.11.4 

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    Event date: 2024.11.2 - 2024.11.4

    Presentation type:Oral presentation (invited, special)  

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  • 味蕾における古典的シナプスの研究

    堀江謙吾, 美藤純弘, 吉田竜介

    第66回歯科基礎医学会学術大会  2024.11.3 

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    Event date: 2024.11.2 - 2024.11.4

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  • うま味受容体欠損マウスを用いた味覚唾液反射経路の検討

    望月晴, 堀江謙吾, 美藤純弘, 吉田竜介

    日本解剖学会第 78 回中国・四国支部学術集会  2024.10.20 

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    Event date: 2024.10.19 - 2024.10.20

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  • Membrane properties and orexin-induced responses in the rat superior salivatory nucleus neurons

    Yoshihiro Mitoh, Kengo Horie, Ryusuke Yoshida

    The 58th Annual Meeting of the Japanese Association for the Study of Taste and Smell  2024.9.11 

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    Event date: 2024.9.11 - 2024.9.13

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  • Distribution of the superior salivatory nucleus neurons innervating the salivary glands, blood vessels of the tongue, and lacrimal glands and the responsivenesses to orexin

    Yoshihiro Mitoh, Kengo Horie, Tadasu Sato, Takehiro Yajima, Hiroyuki Ichikawa, Ryusuke Yoshida

    The 101st Annual Meeting of The Physiological Society of Japan  2024.3.29 

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    Event date: 2024.3.28 - 2024.3.30

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  • Enantiomers of organic acids are identified by the preference taste receptors Tas1R2/Tas1R3 or Tas1R1/Tas1R3

    Yuko Yamase, Hai Huang, Yoshihiro Mitoh, Kengo Horie, Ryusuke Yoshida

    The 20th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception  2023.11.26 

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    Event date: 2023.11.25 - 2023.11.26

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  • SNAP-25 contributes to the maintenance of Type III taste receptor cells

    Kengo Horie, Hai Huang, Kuanyu Wang, Yu Zuo, Keiko Yasumatsu, Yuzo Ninomiya, Yoshihiro Mitoh, Ryusuke Yoshida

    The 20th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception  2023.11.26 

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    Event date: 2023.11.25 - 2023.11.26

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  • Ccn3 expression in the murine taste bud does not confer essential roles in taste perception

    Kuanyu Wang, Yoshihiro Mitoh, Kengo Horie, Ryusuke Yoshida

    The 20th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception  2023.11.26 

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    Event date: 2023.11.25 - 2023.11.26

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  • Analysis of mice lacking synapses in taste buds

    Ryusuke Yoshida, Kengo Horie, Yoshihiro Mitoh

    The 65th Annual Meeting of Japanese Association for Oral Biology  2023.9.18 

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    Event date: 2023.9.16 - 2023.9.18

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  • Effects of dopamine on the superior salivatory nucleus neurons innervating the salivary glands in rats

    Yoshihiro Mitoh, Tadasu Sato, Takehiro Yajima, Kengo Horie, Hiroyuki Ichikawa, Ryusuke Yoshida

    The 65th Annual Meeting of Japanese Association for Oral Biology  2023.9.18 

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    Event date: 2023.9.16 - 2023.9.18

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  • 唾液緩衝能が味覚に及ぼす影響について

    The 56th Annual Meeting of the Japanese Association for the Study of Taste and Smell  2023.9.13 

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    Event date: 2023.9.11 - 2023.9.13

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  • CN3 expression in the murine taste bud does not confer essential roles in taste perception

    The 56th Annual Meeting of the Japanese Association for the Study of Taste and Smell  2023.9.13 

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    Event date: 2023.9.11 - 2023.9.13

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  • 甘味・うま味受容体が有機酸の光学異性体の識別に関与している

    The 56th Annual Meeting of the Japanese Association for the Study of Taste and Smell  2023.9.12 

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    Event date: 2023.9.11 - 2023.9.13

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  • 糖刺激による頭相インスリン分泌の誘発

    The 56th Annual Meeting of the Japanese Association for the Study of Taste and Smell  2023.9.12 

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    Event date: 2023.9.11 - 2023.9.13

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  • Expression of taste related genes in the central chemoreceptor area of mouse brain

    Yuri Namba, Yoshihiro Mitoh, Suzuka Yoshida, Yohei Takeshita, Junichi Asaumi, Ryusuke Yoshida

    The 46th Annual Meeting of the Japan Neuroscience Society  2023.8.2 

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    Event date: 2023.8.1 - 2023.8.4

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  • Elucidation of the mechanism of the gustatory salivary reflex using mice: c-Fos expression in the central nervous system.

    Adachi Reimi, Mitoh Yoshihiro, Yoshida Ryusuke

    The 100th Annual Meeting of the Physiological Society of Japan  2023.3.14 

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    Event date: 2023.3.14 - 2023.3.16

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  • Relationship between salivary buffering capacity and taste sensitivity in humans

    Aiko Hyodo, Yoshihiro Mitoh, Ryusuke Yoshida

    The 100th Annual Meeting of the Physiological Society of Japan  2023.3.14 

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    Event date: 2023.3.14 - 2023.3.16

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  • 発酵乳成分におけるマウスの味覚応答の解析

    山瀬裕子, 黄海, 美藤純弘, 江草正彦, 宮脇卓也, 吉田竜介

    第43回岡山歯学会  2022.12.11 

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    Event date: 2022.12.11

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  • 唾液腺支配の上唾液核ニューロンに対するドーパミンの影響

    美藤純弘、佐藤匡、矢島健大、小橋基、市川博之、吉田竜介

    第 55 回 日本栄養•食糧学会 中国•四国支部大会  2022.10.30 

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    Event date: 2022.10.29 - 2022.10.30

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Excitatory effects of dopamine on the superior salivatory nucleus neurons innervating the rat submandibular and sublingual salivary glands

    Yoshihiro Mitoh, Tadasu Sato, Takehiro Yajima, Motoi Kobashi, Hiroyuki Ichikawa, Ryusuke Yoshida

    The 56th Annual Meeting of the Japanese Association for the Study of Taste and Smell  2022.8.24 

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    Event date: 2022.8.22 - 2022.8.24

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  • Behavioral analysis of taste responses to ingredients in fermented milk in mice

    Yuko Yamase, Huang Hai, Yoshihiro Mitoh, Masahiko Egusa, Takuya Miyawaki, Ryusuke Yoshida

    The 56th Annual Meeting of the Japanese Association for the Study of Taste and Smell  2022.8.22 

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    Event date: 2022.8.22 - 2022.8.24

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  • Immunohistochemical study on the distribution of orexinergic nerve fibers and receptors in the superior salivatory nucleus

    Yoshihiro Mitoh, Tadasu Sato, Takehiro Yajima, Hiroyuki Ichikawa, Motoi Kobashi, Ryusuke Yoshida

    The 98th Annual Meeting of the Physiological Society of Japan  2021.3.30 

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    Event date: 2021.3.28 - 2021.3.30

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  • Mutual interaction of orexin-A and glucagon-like peptide-1 on reflex swallowing in rats

    Motoi Kobashi, Masako Fujita, Yoshihiro Mitoh

    The 62nd Annual Meeting of Japanese Association for Oral Biology  2020.9.11 

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    Event date: 2020.9.11 - 2020.10.9

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  • オレキシンは顎下腺・舌下腺支配の副交感神経節前ニューロンを興奮させる

    美藤純弘, 佐藤 匡, 矢島健太, 藤田雅子, 小橋基, 市川博之, 吉田竜介

    第74回日本栄養・食糧学会大会  2020.5.16 

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    Event date: 2020.5.15 - 2020.5.17

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  • Effects of appetite-boosting peptides on the superior salivatory nucleus neurons in rats

    Yoshihiro Mitoh, Tadasu Sato, Takehiro Yajima, Masako Fujita, Hiroyuki Ichikawa, Motoi Kobashi, Ryusuke Yoshida

    The 97th Annual Meeting of the Physiological Society of Japan  2020.3.19 

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    Event date: 2020.3.17 - 2020.3.19

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  • 上唾液核ニューロンの摂食亢進ペプチドの影響

    美藤純弘, 佐藤匡, 矢島健太, 藤田雅子, 小橋基, 市川博之, 吉田竜介

    第40回岡山歯学会  2019.12.15 

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    Event date: 2019.12.15

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  • Effects of appetite-boosting peptides on the superior salivatory nucleus neurons innervating the salivary glands in rats

    Yoshihiro Mitoh, Tadasu Sato, Masako Fujita, Motoi Kobashi, Hiroyuki Ichikawa, Ryusuke Yoshida

    The 61st Annual Meeting of Japanese Association for Oral Biology  2019.10.14 

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    Event date: 2019.10.12 - 2019.10.14

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  • Effects of appetite-boosting peptides on parasympathetic preganglionic neurons innervating the submandibular and sublingual salivary glands in rats.

    Yoshihiro Mitoh, Tadasu Sato, Takehiro Yajima, Masako Fujita, Motoi Kobashi, Hiroyuki Ichikawa, Ryusuke Yoshida

    The 53th Annual Meeting of the Japanese Association for the Study of Taste and Smell  2019.9 

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    Event date: 2019.9.17 - 2019.9.19

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  • Effects of feeding-promoting peptides on excitability of the superior salivatory nucleus neurons

    Yoshihiro Mitoh, Tadasu Sato, Masako Fujita, Hiroyuki Ichikawa, Motoi Kobashi, Ryusuke Yoshida

    9th FAOPS Congress(FAOPS2019)  2019.3.29 

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    Event date: 2019.3.28 - 2019.3.30

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  • Mutual interaction of orexin-A and glucagon-like peptide-1 on reflex swallowing in anesthetized rats

    Motoi Kobashi, Yuichi Shimatani, Masako Fujita, Yoshihiro Mitoh, Ryuji Matsuo

    9th FAOPS Congress(FAOPS2019)  2019.3.28 

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    Event date: 2019.3.28 - 2019.3.30

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  • Effects of peptides promoting food intake on the superior salivatory nucleus neurons innervating the submandibular and sublingual salivary glands

    Yoshihiro Mitoh, Tadasu Sato, MasakoFujita, Hiroyuki Ichikawa, Motoi Kobashi

    The 60rd Annual Meeting of Japanese Association for Oral Biology  2018.9.18 

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    Event date: 2018.9.5 - 2018.9.7

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  • GLP-1 はオレキシンA による嚥下減弱作用を抑制する

    小橋 基, 島谷 祐一, 藤田 雅子, 美藤 純弘, 松尾 龍二

    第95回日本生理学会大会  2018.3.28 

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    Event date: 2018.3.28 - 2018.3.30

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  • Orexin-A diminishes the suppressive effect of GLP-1 on the reflex swallowing

    2017 

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  • オレキシン A は GLP-1 による嚥下減弱作用を抑制する

    第94回日本生理学会大会  2017 

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  • Orexins excite parasympathetic preganglionic neurons in the superior salivatory nucleus innervating the salivary glands.

    17th International Symposium on Olfaction and Taste (ISOT2016)  2016 

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  • GLP-1 suppresses reflex swallowing via the medial part of nucleus tractus solitarius

    2015 

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  • GLP-1は孤束核内側部を介して反射性嚥下を抑制する

    第92回日本生理学会大会  2015 

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  • GLP-1 の嚥下反射減弱作用に及ぼすオレキシン A の効果

    日本味と匂学会第49回大会  2015 

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  • オレキシンはラット顎下唾液腺を支配する上唾液核ニューロンを興奮させる

    第56回歯科基礎医学会学術大会・総会  2014 

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  • GLP-1 中枢投与による嚥下反射におよぼす作用

    第91回日本生理学会大会  2014 

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  • Effects of orexins on parasympathetic preganglionic neurons in the superior salivatory nucleus innervating the salivary glands.

    2014 

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  • Identification of the effective site of GLP-1 to suppress reflex swallowing.

    2014 

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  • 顎下腺・舌下腺を支配する上唾液核ニューロン活動の摂食中枢による調節

    第35回岡山歯学会総会・学術集会  2014 

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  • 渋味溶液の舌刺激による舌神経と鼓索神経の応答と温度覚との関連

    第56回歯科基礎医学会学術大会・総会  2014 

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  • GLP-1 中枢投与による反射性嚥下抑制を惹起する部位の同定

    日本味と匂学会第48回大会  2014 

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  • 上唾液核における唾液腺支配の副交感神経節前ニューロンに対するオレキシンの影響

    第91回日本生理学会大会  2014 

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  • GLP-1中枢投与による反射性嚥下修飾作用

    日本味と匂学会第46回大会  2013 

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  • ラット顎下腺・舌下腺を支配する上唾液核ニューロンに対するオレキシンの興奮作用

    第55回歯科基礎医学会学術大会・総会  2013 

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  • Developmental Switch of GABA Action in Superior Salivatory Nucleus Neurons.

    第3回岡山医療教育研究シンポジウム  2013 

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  • ラット中枢 GLP-1 の反射性嚥下におよぼす作用

    第55回歯科基礎医学会学術大会・総会  2013 

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  • タンニン酸の舌刺激に対するラット舌神経と鼓索 神経の応答

    第55回歯科基礎医学会学術大会・総会  2013 

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  • オレキシン-A は孤束核交連部のオレキシン 1 受容体を介して嚥下反射を抑制する

    第90回日本生理学会大会  2013 

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  • オレキシン-Aによる反射性嚥下抑制を惹起する中枢作用部位の検討

    日本味と匂学会第46回大会  2012 

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  • ラット大脳皮質の電気刺激で誘発されるリズミカルな顎運動と唾液分泌の関連

    第54回歯科基礎医学会学術大会・総会  2012 

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  • 嚥下反射抑制におよぼすオレキシン A 作用部位の検討

    第89回日本生理学会  2012 

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  • ラット中枢オレキシンによる反射性嚥下の抑制

    第88回日本生理学会大会 (震災のため中止、誌上開催)  2011 

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  • オレキシンはラット顎下腺・舌下腺を支配する上唾液核ニューロンの興奮性を促進する

    第53回歯科基礎医学会学術大会・総会  2011 

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  • オレキシン中枢投与による嚥下反射の抑制

    日本味と匂学会第45回大会  2011 

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  • オレキシン中枢投与による反射性嚥下の抑制

    第53回歯科基礎医学会学術大会・総会  2011 

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  • ラット大脳皮質咀嚼野の電気刺激が誘発するリズミカルな顎運動と唾液分泌の関連

    第53回歯科基礎医学会学術大会・総会  2011 

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  • ラット上唾液核へのGABA作動性抑制性入力に関する免疫組織化学的研究

    第52回歯科基礎医学会学術大会・総会  2010 

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  • ラット上唾液核ニューロンに対するセビメリンの興奮性作用に関する電気生理学的および免疫組織化学的解析

    第51回 歯科基礎医学会学術大会・総会  2009 

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  • Central ghrelin induces proximal stomach relaxation and inhibits reflex swallowing in the anaesthetized rats

    グレリン国際シンポジウム2009  2009 

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  • Central ghrelin induces gastric relaxation of the proximal stomach via the caudal dorsal vagal complex in the rat

    The 36th Congress of the International Union of Physiological Sciences  2009 

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  • Cevimeline enhances the excitability in the rat superior salivatory nucleus neurons

    The 36th Congress of the International Union of Physiological Sciences  2009 

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  • Cevimeline enhances the excitability in the rat superior salivatory nucleus

    The 11th International Symposium on Exocrine Secretion Tokushima 09-Exocrine Secretion-Mechanism and Disease  2009 

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  • Postnatal development of inhibitory synaptic transmission to the superior salivatory neurons in rats

    The 11th International Symposium on Exocrine Secretion Tokushima 09-Exocrine Secretion-Mechanism and Disease  2009 

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  • 中枢摂食亢進ペプチドによる麻酔下ラット胃近位部の弛緩応答

    第7回せとうち消化管運動研究会  2009 

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  • Immunohistochemical study on the Distribution and origin of GABAergic nerve terminals in the superior salivatory nucleus

    The 11th International Symposium on Exocrine Secretion Tokushima 09-Exocrine Secretion-Mechanism and Disease  2009 

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  • GABAergic nerve terminals in the superior salivatory nucleus; immunohistochemical and retrograde tracing study

    The 36th Congress of the International Union of Physiological Sciences  2009 

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  • ラット上唾液核における抑制性受容体と抑制性ニューロンの中枢分布に関する免疫組織化学的研究

    第51回 歯科基礎医学会学術大会・総会  2009 

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  • ラット上唾液核に投射するGABA作動性ニューロンの分布

    第85回日本生理学会大会  2008 

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  • セビメリンはラット上唾液核ニューロンの興奮性を促進する

    第50回 歯科基礎医学会  2008 

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  • 中枢摂食亢進ペプチドによる嚥下の調節作用

    「咀嚼・嚥下」研究発表会 咀嚼・嚥下の生理学的メカニズム、神経性調節の解明  2008 

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  • ラット上唾液核に投射する抑制性ニューロンの分布

    第50回 歯科基礎医学会  2008 

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  • ラット脳スライスにおける最後野ニューロン活動に対するムスカリン受容体活性化による興奮性および抑制性効果

    第85回日本生理学会大会  2008 

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  • プレートの厚みおよび噛みしめ様式がヒトのヒラメ筋H反射に及ぼす影響

    第85回日本生理学会大会  2008 

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  • ラット上唾液核に投射するGABA作動性ニューロンの分布

    第85回日本生理学会大会  2007 

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  • ラット中枢グレリンによる反射性嚥下の調節作用

    第49回 歯科基礎医学会  2007 

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  • ラット脳スライス標本における延髄最後野ニューロンのセピメリンによる興奮性応答

    第49回 歯科基礎医学会  2007 

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  • ラット顎下腺の自律神経中を走行する求心性神経の応答特性

    第49回 歯科基礎医学会  2007 

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  • 上喉頭神経由来の反射性嚥下におよぼす中枢グレリンの調節作用

    日本味と匂学会第41回大会  2007 

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  • 最後野におけるP2X受容体の局在とその機能

    第49回歯科基礎医学会サテライトシンポジウムⅧ 「口腔機能の中枢制御-電気生理学的アプローチ-」  2007 

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  • 中枢摂食亢進ペプチドによるラット胃近位部の弛緩応答

    第49回日本平滑筋学会総会  2007 

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  • ラット脳スライスにおける最後野ニューロン活動に対するムスカリン受容体活性化による興奮性および抑制性効果

    第85回日本生理学会大会  2007 

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  • プレートの厚みおよび噛みしめ様式がヒトのヒラメ筋H反射に及ぼす影響

    第85回日本生理学会大会  2007 

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  • Electrophysiological study of the inhibitory inputs from the forebrain and brainstem to the superior salivatory nucleus in rats.

    3rd International symposium on salivary glands in honor of Niels Stensen.  2006 

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  • ニューロペプチドYの胃近位部弛緩におよぼす中枢性作用

    第83回日本生理学会大会  2006 

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  • Electrophysiological study on the descending excitatory synaptic inputs to the superior salivatory nucleus in the rat.

    3rd International symposium on salivary glands in honor of Niels Stensen.  2006 

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  • ラット脳スライス中の孤束膠様核ニューロンの形態学的特徴と化学感受性

    第83回日本生理学会大会  2006 

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  • 圧刺激に反応するラット顎下腺からの求心性情報の電気生理学的解析

    第83回日本生理学会大会  2006 

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  • 種々の食物摂取によるラット顎下腺唾液分泌の測定

    第83回日本生理学会大会  2006 

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  • ラット最高野ニューロンにおける細胞外ATPの膜電位および自発発火に対する効果

    第83回日本生理学会大会  2006 

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  • ラット上唾液核細胞における抑制性シナプス伝達の発達

    第83回日本生理学会大会  2006 

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  • ラット上唾液核細胞に対する下行性の興奮性および抑制性入力に関する電気生理学的研究

    平成16年度生理研研究会「唾液分泌機構解明に向けての戦略的展開」  2005 

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  • 自律系ニューロンのおもしろさ:延髄最後野単一ニューロンの形態および機能の多様性

    第82回日本生理学会大会  2005 

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  • ラット顎下腺に分布する感覚神経の入力経路に関する組織化学的研究

    第25回岡山歯学会総会  2005 

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  • 飼料摂食時のラット顎下腺唾液分泌量と咀嚼筋活動の分析

    第47回 歯科基礎医学会  2005 

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  • 最後野におけるプリン受容体(P2X)による神経活動の修飾

    第47回 歯科基礎医学会  2005 

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  • 孤束膠様核単一ニューロンの形態的特徴,膜特性および化学感受性

    第47回 歯科基礎医学会  2005 

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  • 舌および顎下腺・舌下腺を支配するラット上唾液核細胞における抑制性シナプス伝達の生後発達

    第47回 歯科基礎医学会  2005 

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  • 迷走神経複合核のニューロペプチドY-Y1受容体を介した胃近位部弛緩の解析

    第47回 歯科基礎医学会  2005 

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  • ラット最高野ニューロンの機能と形態

    第85回日本生理学会北海道地方会  2005 

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  • ニューロペプチドY-Y1受容体を介した胃近位部の食物受け入れ応答

    日本味と匂学会第39回大会  2005 

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  • ラット上唾液核ニューロンに対する下行性の興奮性シナプス入力に関する電気生理学的検討

    第82回日本生理学会大会  2005 

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  • ラット上唾液核細胞に対する下行性抑制性シナプス入力に関する電気生理学的解析

    第82回日本生理学会大会  2005 

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  • 脳スライス標本における最後野ニューロンのATP受容体を介する興奮性の修飾

    第82回日本生理学会大会  2005 

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  • ラット顎下腺求心性神経の経路に関する組織化学的研究

    第82回日本生理学会大会  2005 

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  • The excitatory inputs to the superior salivatory neurons

    Japanese Association for Dental Research 53rd Annual Meeting  2005 

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  • The inhibitory synaptic inputs to the superior salivatory nucleus

    Japanese Association for Dental Research 53rd Annual Meeting  2005 

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  • Modulation of area postrema neuronal excitability via P2X purinoceptors in rat brain slices.

    第35回北米神経科学会  2005 

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  • Functional interaction between neurons in the area postrema and in the nucleus gelatinosus solitarius.

    第35回北米神経科学会  2005 

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  • 口腔領域の刺激によるラット顎下腺唾液分泌の神経生理学的研究

    第81回日本生理学会大会  2004 

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  • ラット上唾液核細胞のシナプス入力に関する電気生理学的解析

    第46回歯科基礎医学会学術大会ならびに総会  2004 

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  • ラット顎下腺に分布する感覚神経の経路に関する組織化学的研究

    第46回歯科基礎医学会学術大会ならびに総会  2004 

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  • 最高野ニューロンにおける過分極作動性カチオン電流(Ih)のプロポフォールに対する感受性

    第44回歯科基礎医学会学術大会ならびに総会  2004 

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  • ラット顎下腺からの求心性情報の電気生理学的解析

    第46回歯科基礎医学会学術大会ならびに総会  2004 

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  • 自律神経支配から見た唾液分泌機構ー電気生理学的解析

    第46回歯科基礎医学会学術大会若手サテライトシンポジウム 「唾液腺機能制御機構解析法の最前線線」  2004 

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  • ラット上唾液核細胞における興奮性の反復シナプス入力に対する発火特性

    第46回歯科基礎医学会学術大会ならびに総会  2004 

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  • 顎下腺・舌下腺および舌を支配するラット上唾液核ニューロンのシナプス応答

    第81回日本生理学会大会  2004 

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  • ラット上最高野ニューロンの電気生理学的および形態学的特徴

    第81回日本生理学会大会  2004 

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  • ラット最高野ニューロンのセロトニン(5-HT)感受性

    第76回日本薬理学年会・第80回日本生理学会大会  2003 

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  • ELECTROPHYSIOLOGICAL ANALYSIS OF THE AFFERENT NEURAL ACTIVITY FROM THE SUBMANDIBULAR SALIVARY GLAND IN RATS.

    Society for Neuroscience 33rd Annual Meeting 2003  2003 

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  • Facilitation of glutamate release by the activation of presynaptic 5-HT3 receptors in rat area postrema neurons.

    Society for Neurosience 33rd Annual Meeting  2003 

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  • ラット上唾液核の抑制生シナプス後電流はシナプス後膜のGABAB受容体を介して調節される。

    第76回日本薬理学年会・第80回日本生理学会大会  2003 

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  • ラット上唾液核細胞における抑制性シナプス伝達はGABAB受容体を介する

    第44回歯科基礎医学会学術大会ならびに総会  2002 

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  • 唾液腺を支配するラット上唾液核細胞の抑制性入力の解析

    第79回日本生理学会大会  2002 

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  • ラット最高野ニューロンにおけるH電流の特性

    第79回日本生理学会大会  2002 

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  • Suppression of the hyperpolarization-activated inward current contributes to the inhibitory actions of propofol on the epileptiform activities of hippocampal neurons.

    第32回北米神経科学  2002 

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  • 最高野におけるセロトニン(5-HT)受容体による神経活動の調節メカニズム

    第23回岡山歯学会総会  2002 

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  • 過分極作動性内向き電流(Ih)によるラット最後野神経活動の制御機構

    第44回歯科基礎医学会学術大会ならびに総会  2002 

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  • Excitability of area postrema neurons controlled by the hyperpolarization-activated inward currents.

    第32回北米神経科学  2002 

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  • PropofolのH電流抑制作用に関する電気生理学的検討

    第79回日本生理学会大会  2002 

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  • 海馬CA1ニューロンのH電流に対するプロポフォールの抑制作用

    第43回歯科基礎医学会総会  2001 

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  • ラット上唾液核細胞の興奮性および抑制性入力の解析

    第15回唾液腺談話会  2001 

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  • スライスパッチクランプ法によるラット最高野ニューロンの膜特性解析

    第43回歯科基礎医学会総会  2001 

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  • Excitatory and inhibitory postsynaptic currents recorded from the superior salivatory neurons in rats.

    International Society for Autonomic Neuroscience (ISAN)  2000 

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  • Electrophysiological analysis of sharp-wave associated gamma-frequency ocillation in the retorohippocampal cortices and amygdala in rat brain slice.

    International Society for Autonomic Neuroscience (ISAN)  2000 

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  • てんかん様バースト発火に対するプロポフォールの抑制機序の検討

    第42回歯科基礎医学会総会  2000 

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  • ラット脳スライスを用いた海馬CA1ニューロンのバースト発火に対するプロポフォールの抑制機序の検討

    第21回岡山歯学会総会  2000 

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  • 舌、顎下腺・舌下腺を支配するラット上唾液核細胞のシナプス入力の解析

    第42回歯科基礎医学会総会  2000 

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  • 海馬体および扁桃体ニューロンの膜特性および神経連絡網

    海馬体および扁桃体ニューロンの膜特性および神経連絡網  2000 

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  • Role of the parabrachial nucleus in salivation induced by unpalatable taste in rats.

    International Society for Autonomic Neuroscience (ISAN)  2000 

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  • Enhancement of synaptic transmission induced by burst responses of the deep layer neurons in the entrohinal cortex and the parasubiculum.

    第23回神経科学大会・第10回神経回路学会大会合同大会  2000 

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  • カエル舌咽神経の塩応答に対するアミロライドの効果

    第31回東北生理談話会  1999 

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  • 海馬体および扁桃体ニューロンの膜特性および神経連絡網

    第41回歯科基礎医学会学術大会ならびに総会  1999 

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  • 舌、顎下腺・舌下腺を支配するラット上唾液核細胞の形態学的特徴と膜特性

    第20回岡山歯学会総会  1999 

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  • 扁桃体単一ニューロン特性および海馬体との神経連絡に関する電気生理学的検討

    第76回日本生理学会大会  1999 

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  • 舌と顎下腺・舌下腺を支配するラット上唾液核細胞の形態学的特徴

    第41回歯科基礎医学会学術大会ならびに総会  1999 

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  • 舌、顎下腺及び舌下腺を支配する上唾液核細胞の形態学的解析

    第76回日本生理学会大会  1999 

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  • Effects of appetite-boosting peptides on the superior salivatory nucleus neurons in rats

    Yoshihiro Mitoh, Tadasu Sato, Takehiro Yajima, Masako Fujita, Hiroyuki Ichikawa, Motoi Kobashi, Ryusuke Yoshida

    The 97th Annual Meeting of the Physiological Society of Japan 

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    Language:English   Presentation type:Poster presentation  

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Research Projects

  • Elucidation of the mechanism of taste cell shedding and its application to model mice for neurological disorders

    Grant number:24K22186  2024.06 - 2027.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    吉田 竜介, 美藤 純弘, 堀江 謙吾

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    Grant amount:\6370000 ( Direct expense: \4900000 、 Indirect expense:\1470000 )

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  • Protection of the salivary gland during radiotherapy for head and neck cancer

    Grant number:24K13156  2024.04 - 2027.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    美藤 純弘, 竹下 洋平, 久富 美紀, 安尾 敏明

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

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  • 口腔機能低下症に迷走神経刺激は有効か:脳腸連関によるニューロモジュレーション解析

    Grant number:22K10246  2022.04 - 2025.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    植田 紘貴, 吉田 竜介, 美藤 純弘

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

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  • 新技術を用いた革新的包括的自律神経研究の拠点形成

    2021.10 - 2022.03

    岡山大学  「令和3年度岡山大学次世代研究育成グループ」事業 

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\500000

  • The raison d'etre of classical synapses in taste buds

    Grant number:21H03106  2021.04 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    吉田 竜介, 美藤 純弘, 樽野 陽幸, 池亀 美華

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    Grant amount:\17420000 ( Direct expense: \13400000 、 Indirect expense:\4020000 )

    本年度は、本研究で用いる以下の遺伝子改変マウスを作成した。(1)味蕾においてGABA合成酵素であるGAD67を欠損するマウス(K5-Cre;GAD67-flox)、(2)味蕾においてGABA合成酵素であるGAD67を欠損しGFPを発現するマウス(K5-Cre;GAD67-GFP/flox)、(3)味蕾においてシナプス関連遺伝子SNAP25を欠損するマウス(K5-Cre;SNAP25-flox)、(4)味蕾内でIII型細胞のみで発現する遺伝子を欠損するマウス2種(TypeIII-A-KO、TypeIII-B-KO)。今後、(3)または(4)かつGAD67-GFPを発現するマウス、(1)~(4)かつTRPV1を欠損するマウスを順次作成する予定である。また、K5-Creの味蕾での発現を確認するため、GAD67-GFPを発現し、K5発現細胞でtomatoの発現を誘導するマウス(K5-Cre;ROSA26-tomato;GAD67-GFP)を作成し、GAD67発現細胞(III型細胞)を含む味蕾細胞でK5-Creが発現することを確認した。さらに免疫組織化学的実験により、(2)のマウスにおいて味蕾内GFP発現細胞でGAD67発現が消失し、他の味細胞マーカー遺伝子の発現は変化しないことを確認した。
    次に、これらマウスを作成するために用いた各種遺伝子改変マウス(K5-Cre、GAD67-flox、SNAP25-flox、TRPV1-KO)の味覚行動応答について短時間リック法にて調べた。その結果、これらのマウスは各種味溶液(ショ糖、キニーネ、NaCl、グルタミン酸ナトリウム、HCl、クエン酸など)に対し野生型マウスとほぼ同様の応答を示すことを確認した。ただし、TRPV1-KOマウスに関してはカプサイシンに対する忌避反応が減弱していた。

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  • The raison d'etre of classical synapses in taste buds

    Grant number:23K21484  2021.04 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    吉田 竜介, 美藤 純弘, 樽野 陽幸, 池亀 美華

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    Grant amount:\17420000 ( Direct expense: \13400000 、 Indirect expense:\4020000 )

    本年度は、これまでに作成した味蕾においてGABA合成酵素を欠失するマウス(GAD67-cKOマウス)、味蕾においてシナプス関連遺伝子SNAP25を欠損するマウス(SNAP25-cKOマウス)、およびそれらのコントロールマウスを用い、各種味刺激に対する応答を記録した。GAD67-cKOマウスはコントロールマウスと比較し、甘味、苦味、うま味、酸味、塩味刺激に対する鼓索神経応答については有意な差が見られなかった。これは、これまで得られた行動学的実験結果とも一致する。一方、SNAP25-cKOマウスでは、通常応答の標準化に用いる塩化アンモニウムに対する応答が減少しており、代りに甘味溶液を標準化溶液として用いた。その結果、酸味に対する応答がほぼ消失し、他の味刺激に対する応答はコントロールマウスと有意な差は見られなかった。行動学的実験では、SNAP25-cKOのみでは酸に対する忌避応答が若干減少するのみであったが、侵害受容体であるTRPV1も同時に欠損するダブルノックアウトマウスでは酸味の忌避応答がかなり減弱した。しかし高濃度酸溶液に対してはまだ野生型と同様の忌避を示した。以上の結果、味蕾内のGAD67については、直接的な味覚情報伝達には寄与せず、SNAP25は酸味および塩化アンモニウムの味覚応答の伝達に必須である可能性が示された。また、別途、味蕾内でシナプスを発現する細胞に特異的に発現する新たな分子としてCCN3を見出したが、そのノックアウトマウスの行動応答や神経応答を解析したところ目立った表現型の変化は見られなかった。CCN3は新たな味細胞マーカーとして用いることが可能であるが、その機能については未だ不明である。

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  • おいしく食べるための唾液、その中枢神経機構の解明

    Grant number:21K11697  2021.04 - 2024.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    美藤 純弘, 向井 康敬

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    Grant amount:\4030000 ( Direct expense: \3100000 、 Indirect expense:\930000 )

    マウスは遺伝子組換えの種類が豊富なので、本研究課題はマウスを使った測定系を確立し分析を進める予定であった。しかし、共同研究者の向井らがオレキシンCreラットを開発したので、これまでのラットにおける研究成果を生かすためにもラットで行うことにした。本研究ではラット視床下部外側野ニューロンを電気刺激あるいは光刺激したときの上唾液核ニューロンの応答や唾液分泌を調べる予定であるが、そのためには視床下部外側野に電気刺激または光刺激するための光ファイバーを刺入する位置を特定する必要がある。そこで上唾液核ニューロンに投射する視床下部外側野ニューロンの分布を検討するために、上唾液核に蛍光色素を投与し、逆行性軸索輸送により視床下部外側野ニューロンを染色した。上唾液核(吻尾側方向に500マイクロメートル、背腹方向に500マイクロメートル)に局所的に色素注入するのは精密な技術を要するためにまだ成功例が少ないが(n=2)、標識ニューロンは視床下部外側野において吻尾側方向に広範囲に渡って分布しているように思われた。この標識ニューロンのうちオレキシン産生ニューロンはどれくらい割合で存在し、またどのように分布しているか、オレキシンに対する免疫染色を行うことにより検討中である。この実験は本研究において重要な部分で、刺激部位について確証を得るために現在集中的に行なっている。一方、オレキシンニューロンを特異的に興奮させるために、470 nm光で細胞を活性化させるチャネルロドプシン2をオレキシンニューロンに組み込むことを試みている。

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  • A study on brain mechanisms in which mental activity influences salivary secretion

    Grant number:18K11022  2018.04 - 2021.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Mitoh Yoshihiro

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    The effects of dopamine (DA), serotonin (5HT), and noradrenaline (NA) on the rat superior salivary nucleus neurons, which is the parasympathetic center of the submandibular and sublingual salivary glands, were investigated using slice patch clamp method. DA and 5HT generated inward currents, but not NA. DA also generated outward currents. 5HT and NA increased the frequency of miniature excitatory postsynaptic currents, but not DA. Immunohistochemical studies for DA receptors demonstrated that the neurons had immunoreactivities for D1-like receptors involved in excitatory effects, and those for D2-like receptors involved in inhibitory effects. These results suggest that salivary secretion may change with mental condition.

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  • 精神活動により影響される唾液分泌に関する研究

    Grant number:17K00921  2017.04 - 2020.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    藤田 雅子, 美藤 純弘, 松尾 龍二, 小橋 基

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

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  • Study for regulatory mechanism of feeding-related phenomena by GLP-1

    Grant number:15K00818  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Kobashi Motoi

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    Grant amount:\4680000 ( Direct expense: \3600000 、 Indirect expense:\1080000 )

    The effects of glucagon like peptide-1 (GLP-1), which is appetite-reducing peptide, on reflex swallowing were examined using anaesthetized rats. GLP-1 suppressed reflex swallowing via the medial part of the NTS in the dorsal medulla. Orexin-A, which is appetite enhancing peptide, also suppressed reflex swallowing. The administration of orexin-A prior to the injection of GLP-1 attenuated the degree of suppression of the swallowing response induced by GLP-1-administration. Suppression of reflex swallowing by appetite-related peptides is thought to be helpful in keeping the ability of reflex swallowing constant in cooperation with the suppression ofappetite-enhancing peptides.

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  • Neural mechanisms of salivary secretion related to oral senses and mastication.

    Grant number:26462811  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    MATSUO Ryuji, Carpenter Guy H.

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    Grant amount:\4810000 ( Direct expense: \3700000 、 Indirect expense:\1110000 )

    The neural mechanism for salivary secretion induced by mastication and oral senses was investigated by electrophysiological techniques in the rat. As the result, we found that oral senses are cue sign for starting and determining patterns of both mastication and salivation. This suggests that salivation is not a result of mastication or oral sensory information. Secreted saliva dissolves foods and helps to make sense of taste, affecting taste quality and intensity. Moreover, saliva protects taste receptors in the oral cavity. Mastication, salivation, and oral senses are affected each other.

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  • A study on mechanisms regulating salivary secretion during feeding

    Grant number:26500009  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    FUJITA Masako

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    It is most likely that salivary secretion during feeding is regulated by the lateral hypothalamic area (LH). We investigated the effects of melanin concentrating hormone (MCH) which is a feeding promoting peptide localized in the LH, on superior salivatory nucleus (SSN) neurons, which is the primary center of the parasympathetic nervous systems for the submandibular and sublingual glands. No SSN neurons exhibited immunoreactivity for MCH receptor and responded to MCH application. However, in the same recording neurons, orexin, a feeding promoting peptide localized in the LH as with MCH, showed excitatory responses. Therefore, regulation of SSN neuronal activity by LH would be conducted by other transmitters including orexin.

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  • Approach to Educate HACCP in Schools of Medicine, Dentistry and Pharmacy

    Grant number:26670261  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    Kato Masahiko, KONNO Katsuhiko

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    Grant amount:\2860000 ( Direct expense: \2200000 、 Indirect expense:\660000 )

    A questionnaire on hazard analysis and critical control points (HACCP) was conducted among students in schools of dentistry and pharmacy in 2014. The results suggested that students did not have adequate understanding of HACCP. We gave training on HACCP application to pharmacy students in 2015 using commercially available textbooks. After the training, we collected student-written documents and distributed another questionnaire on HACCP. The next year (2016), we then performed the training and evaluation a second time using textbooks that we created ourselves. The number of errors in the student-written documents was higher and the average score on the questionnaire was lower in 2015 than in 2016, suggesting that our textbook is effective. We have revised our textbook accordingly, which we are publishing as “HACCP for Students”.

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  • A study for role of the feeding center in submandibular and sublingual salivary secretion

    Grant number:25462887  2013.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Mitoh Yoshihiro, MATSUO RYUJI, KOBASHI MOTOI, FUJITA MASAKO

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    Grant amount:\4940000 ( Direct expense: \3800000 、 Indirect expense:\1140000 )

    Using free-moving rats, we examined the salivation when rats were provided with diets with various water contents. In sham-operated rats, salivation was decreased with increase of water contents in diets (powder > hard pellet > hard mash > medium mash > soft mash). Lesions of feeding center largely reduced the salivation although the tendency for salivary volume was maintained. In the superior salivatory nucleus (SSN) neurons which is the primary parasympathetic center of the submandibular and sublingual salivary glands in rats, effects of orexins and melanin-concentrating hormone (MCH), which were a feeding stimulator that were specifically produced in the lateral hypothalamic area (feeding center). SSN neurons were excited by orexins via orexin receptor 1 and 2 with decrease of potassium conductance, but not MCH. Excitation of SSN neurons via orexin may be involved in abundant salivation in feeding.

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  • Analysis of neural network for maintenance salivary secretion

    Grant number:23592731  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    MATSUO RYUJI, KOBASHI Motoi, TERAYAMA Ryuji, MITOH Yoshihiro, FUJITA Masako

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    Grant amount:\5070000 ( Direct expense: \3900000 、 Indirect expense:\1170000 )

    The neural circuit for regulation of salivary secretion was investigated by anatomical and electrophysiological techniques. Our histochemical study shows that the superior salivatory nucleus in the lower brainstem (parasympathetic neurons innervating the submandibular and sublingual salivary glands) has heavy connection with the lateral hypothalamic area (feeding center). Our electrophysiological study also shows that main neural circuit for vigorous salivation is composed of the cerebral cortex of masticatory area, lateral hypothalamic area, and superior salivatory nucleus. It is suggested that the texture of food is recognized in the cerebral cortex that forms the command for salivation and mastication suitable for the recognized food. This salivary function may be maintained by visceral afferents form the salivary glands.

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  • Central orexin-A inhibits reflex swallowing via caudal brainstem

    Grant number:22592064  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    KOBASHI Motoi, SHIMATANI Yuichi, MITOH Yoshihiro, MATSUO Ryuji, FUJITA Masako

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    We examined the effects of orexins on the reflex swallowing using anaesthetized rats. Orexins were administered into the fourth ventricle. Swallowing was induced by repeated electrical stimulation of the central cut end of the superior laryngeal nerve (SLN) and was identified by the electromyogram lead penetrated the mylohyoide muscle through bipolar electrodes. The frequency of swallowing during the electrical stimulation of the SLN decreased after the administration of orexin-A in a dose-dependent manner. The latency of the first swallowing tended to be extended after the administration of orexin-A. The administration of orexin-B did not affect swallowing frequency. Pre-administration of SB334867, an orexin1 receptor antagonist, attenuated the degree of inhibition of swallowing frequency induced by the administration of orexin-A. To identify the effective site of orexin-A, the effect of a microinjection of orexin-A into the dorsal vagal complex (DVC) was evaluated. Orexin-A was injected into one of the lateral DVC, the intermediate DVC, or the medial DVC. Microinjection of orexin-A into the medial DVC but not the other two sites decreased swallowing frequency. Pre-injection of SB334867 into the medial DVC disrupted the inhibitory response induced by fourth ventricular administration of orexin-A. The electrical lesion of the commissural part of the NTS, but not ablation of the AP, abolished the inhibition of reflex swallowing induced by fourth ventricular administration of orexin-A. These results suggest that orexin-A inhibits reflex swallowing via orexin1 receptors situated in the commissural part of the NTS and/or its vicinity.

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  • Reguraltory mechanisms of neuronal activity in the superior salivatory nucleus by feeding-related peptides.

    Grant number:21592361  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    MITOH Yoshihiro, MATSUO Ryuji, KOBASHI Motoi, FUJITA Masako, ICHIKAWA Hiroyuki

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    The superior salivatory nucleus(SSN) neurons which is the primary parasympathetic center of the submandibular and sublingual salivary glands in rats were excited by orexins, one of feeding-related peptides, from lateral hypothalamic neurons(feeding center). The excitation in SSN neurons was completely inhibited by antagonists for OX1R and OX2R, orexins receptors subtypes. Additionally, SSN neurons showed immunoreactivities for OX1R and OX2R, suggesting that the excitation is mediated by OX1R and OX2R. Orexin-induced excitation in SSN neurons may be involved in abundant salivation in feeding.

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  • Central ghrelin inhibits reflex swallowing and induces gastric relaxation of the rats

    Grant number:19592144  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    KOBASHI Motoi, MATSUO Ryuji, MITOH Yoshihiro, TSUKAMOTO Goichi

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    空腹を起こし、その結果食事の量を増やす作用を持つグレリンという物質の作用を、麻酔を施したラットを用いて検討した。グレリンを脳に投与すると胃の弛緩が生じ、嚥下の頻度が減少することが明らかとなった。胃の弛緩は食事の量が増えても胃に適切に食事が収まるように、胃の容量を広げてやる作用があることを示している。嚥下の頻度の減少の意味を明らかにするには、さらなる研究が必要である。

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  • Study on the glutamatergic inputs from the forebrain and brainstem to the neurons innervating submandibular salivary glands

    Grant number:19592143  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    MITOU Yoshihiro, MATUO Ryuji, KOBASHI Motoi

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    麻酔下ラット唾液分泌神経(上唾液核)にグルタミン酸バイオセンサーを挿入し、味覚や機械刺激に対する応答を分析した。正常および切断除脳を行ったラットのパッチクランプ実験で、上唾液核は口腔感覚情報を中継している下位脳幹からのグルタミン酸入力の他に、前脳からもグルタミン酸入力を受けていることが示唆された。自由行動下のラットで摂食中、摂食中枢にグルタミン酸入力をブロックする薬物を投与すると唾液分泌は減少した。よって、摂食中の唾液分泌には、下位脳幹からの興奮性入力以外に摂食中枢など前脳からの興奮性入力が重要であることが示唆された。

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  • 唾液核に対する興奮性入力の解析-グルタミン酸バイオセンサーによる行動学的測定法一

    Grant number:18659552  2006 - 2007

    日本学術振興会  科学研究費助成事業  萌芽研究

    松尾 龍二, 小橋 基, 美藤 純弘

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    Grant amount:\3300000 ( Direct expense: \3300000 )

    唾液分泌の神経制御は、脳幹部における反射と上位中枢からの下行性制御に区分して論じられている。本研究では、特に摂食行動に関係の深い上位中枢(視床下部外側野、大脳皮質味覚野と咀嚼野)からの興奮性入力(グルタミン酸作働性)を動物実験により検討する。具体的には唾液分泌の副交感神経中枢である上唾液核細胞に対する上位中枢からの興奮性入力量とそれに伴う唾液分泌動態を分析する。とくに本実験計画では、興奮性入力量をグルタミン酸バイオセンサーを用いて解析する。本年度は、1.グルタミン酸バイオセンサーを急性実験と慢性実験に適用した。2.免疫組織学的に上位中枢の興奮性細胞と抑制性細胞を染色した。3.脳スライス標本で唾液核への入力を電気生理学的に検索した。
    1.麻酔下動物(Wistar系雄性ラット)にて、口腔領域の味覚刺激によって唾液分泌を誘発した。同時に上唾液核のグルタミン酸変動をグルタミン酸バイオセンサーで観察し、定量的に解析した。その結果、グルタミン酸バイオセンサーの変動は味覚刺激では小さかった。慢性実験では、とくに摂食行動中に変動が大きく、摂食に関連する中枢(視床下部外側野、扁桃体)の影響が大きいことが示唆された。
    2.免疫組織学的実験により、上唾液核に入力する神経を検索した。その結果、上位中枢(視床下部外側野、扁桃体、大脳皮質味覚野と咀嚼野)には興奮性細胞(グルタミン酸作働性)が多数存在し、抑制性細胞は極めて少数であることが分かった。
    3.電気生理学的に、上唾液核細胞は興奮性と抑制性の入力を受けるが、両者はともに大きな膜電流の変化を発生させた。これは基本的に脳幹部に抑制性神経が多く存在することを示唆するものであった。
    以上の結果より、上唾液核細胞は、摂食行動中に主に上位中枢からは興奮性入力を受け、抑制性入力は脳幹部の介在神経から受けることが示唆された。

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  • Electrophysiological study on development of inhibitory synaptic transmission in central neurons involved in oral function.

    Grant number:17591935  2005 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    MITOH Yoshihiro, MATSUO Ryuji, FUNHASHI Makoto

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    Grant amount:\2800000 ( Direct expense: \2800000 )

    We showed the excitatory (glutamate) and inhibitory (GABA and glycine) synaptic inputs to superior salivatory (SS) neurons in neonatal rat brainstem slices. There is a possibility that the synaptic transmission to SS neurons develop postnatally with the development of oral function. In the present study, we studied the development of the inhibitory synaptic transmission to SS neurons electrophysiologically. We used Wistar rats at postnatal day 2 (P2) to P14. The SS neurons innervating the submandibular salivary glands and tongue were labeled by retrograde axonal transport of a fluorescent dye. Gramicidin perforated patch-clamp recordings from the labeled cell were performed. To examine the reversal potential (V_<rev>), the inhibitory postsynaptic currents (IPSCs) were evoked by electrical stimulation near the recording cell at various potentials. Additionally, to examine the effect of GABA_A receptor activation on the excitability of the cells, GABA (1 mM, 100 ms) was applied via pressure ejection near the recording cell at its resting potential. The V_<rev> was shifted with age, and that at P2-P4, P5-P7 and P8-P14 were-52.6 ± 5.1 mV (n = 9),-62.3 ± 1.6 mV (n = 12) and-71.4 ± 1.5 mV (n = 12), respectively. Exogenous GABA application produced depolarization in 40 % cells at P2-P4, and produced hyperpolarization after P8. Such functional change of synaptic transmission might be involved in the transition from sucking to mastication.

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  • The study for functional correlation between palatability and gastric motility induced by appetite-enhancing peptides.

    Grant number:17591936  2005 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    KOBASHI Motoi, TSUKAMOTO Goichi, MATSUO Ryuji, MITOH Yoshihiro

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    Grant amount:\3400000 ( Direct expense: \3400000 )

    The functional correlation between palatability and gastric motility induced by appetite-enhancing peptides were investigated in this study. The effects of neuropeptide Y (NPY) on motility of the proximal stomach were examined in anaesthetized rats. The administration of NPY into the 4th ventricle induced relaxation of the proximal stomach in a dose-dependent manner. The administrations of Y1 receptor (Y1R) agonist induced relaxation potently rather than those of NPY. The microinjections of Y1R agonist into the caudal part of the dorsal vagal complex (c-DVC) induced relaxation of the proximal stomach. The administration of NPY into the 4th ventricle did not induce relaxation after bilateral injection of Y1R antagonist into the c-DVC. These results revealed that NPY induced the relaxation in the proximal stomach via Y1R situated in the DVC. The effect of ghrelin on motility of the proximal stomach was also examined. The administration of ghrelin into the fourth ventricle induced relaxation of the proximal stomach in a dose-dependent manner. Simultaneous administration of ghrelin and GHS-R antagonist did not induce significant change in IGP. Microinjections of ghrelin into the c-DVC induced relaxation of the proximal stomach. These results indicate that ghrelin induces the relaxation of the proximal stomach via the GHS-R situated in the DVC as well as NPY. These results strongly suggest that the appetite-enhancing peptide facilitate reservoir function of the stomach to accommodate body to overconsumption elicited by the appetite-enhancing neuropeptides. However, the administration of the other appetite-enhancing peptides, melanin-concentrating hormone (MCH), did not induce gastric response. Intracerebroventricular (ICV) administrations of orexin, MCH and NPY increased the intake of saccharin. Drinking of saccharin in turn elevated the mRNA levels of orexin and NPY, but not MCH. Pre-treatments of naloxone, an opioid antagonist, blocked the orexigenic effects of orexin and NPY. These results suggest that the overconsumption promoted by sweet and palatable tastes is attributed to the activation of orexigenic neuropeptides, such as orexin and NPY, and a downstream opioid system together with enhanced digestive functions.

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  • Analysis of the functional and biological roles of the hyperpolarization-activated cation current in the central nervous system,

    Grant number:17591937  2005 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    FUNAHASHI Makoto, SOGAWA Chiharu, MITOH Yoshihiro, SOGAWA Norio, MATSUO Ryuji

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    Grant amount:\3500000 ( Direct expense: \3500000 )

    We performed whole-cell patch-clamp recordings from area postrema neurons to investigate the functional and biological roles of the hyperpolarization-activated cation current (Ih).
    1) Morphological properties
    Using electrophysiological criteria, AP neurons were subdivided into three groups : 1) cells displaying both the hyperpolarization-activated cation current (I_h) and the fast transient outward current (fast I_<to>) ; 2) cells displaying only the fast I_<to> ; and 3) cells displaying only the slow Ito. All AP neurons had a single axon that was distinctly thinner than the cells' dendrites. No systematic differences, across groups, in the orientation of dendrites or axons were identified. Mean values of cell size and capacitance of neurons from group 3 were significantly larger than those of the other groups. Interestingly, a number of cells from group 1 and 3 but not group 2 were found to extend their dendrites into the nucleus tractus solitarius (NTS), suggesting that AP neurons could receive vagal afferent inputs at their dendritic termini within the NTS. Although the AP have been implicated to contain uniformly shaped neurons, this study indicates the presence of significantly different subpopulations of AP neurons, which were characterized not only electrophysiologically but also morphologically.
    2) Sensitivity to ATP
    Most area postrema neurons responded to ATP application, and most responses were excitatory. Voltage-clamp recordings showed three different types of response : 1) a postsynaptic or extrasynaptic excitatory response (inward currents ; n=26/51 cells), 2) a presynaptic excitatory response (increased frequency of miniature excitatory postsynaptic currents with only a small direct postsynaptic current; n=24/51 cells, or 3) a postsynaptic inhibitory response (outward current ; n=1/51). The excitatory responses were found in both of the two major electrophysiological cell classes, i.e. cells displaying I_h and cells not displaying I_h, while the inhibitory responses were found in only cells not displaying I_h. Current-clamp recordings showed ATP-induced depolarization (n=13/15) or hyperpolarization (n=2/15) of membrane potential that modulated the frequency of action potentials. In the presence of CNQX, mEPSCs were abolished and bath applied ATP did not generate mEPSCs, indicating that glutamate release was facilitated by the activation of presynaptically located ATP receptors. Our pharmacological results from studies with ATP, aβme-ATP, βyme-ATP and PPADS indicate that P2X_2, P2X_5 and P2X_7 are the most likely subunits which compose the P2X receptor in the post-and/or extrasynaptic regions. We conclude that half of the presynaptic P2X receptors are most likely composed of P2X_2, P2X_5 and P2X_7 subunits while the others also contain P2X_1 subunits. It is well known that P2X_7 subunit forms only homomultimeric P2X receptors. Finally, the present study suggests that purinoceptor activation may contribute to the control of several autonomic functions by area postrema neurons.

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  • Mechanisms Involving in the Inhibition of Nitric Oxide-Mediated Lower Esophageal Sphincter Relaxation Induced by Volatile Anesthetics in Rabbits.

    Grant number:15592110  2003 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    MITOH Yoshihiro, MIYAWAKI Takuya, MATSUO Ryuji

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    Grant amount:\3500000 ( Direct expense: \3500000 )

    Nonadrenergic noncholinergic (NANC) nerves, which regulate peristaltic waves and/or relaxing mechanisms of the gastrointestinal tract, are known to be nitrergic and to initiate NO-cGMP signaling pathway. We investigated the effect of sevoflurane on NANC relaxation of the lower esophageal sphincter (LES), and the relevance of peripheral N-methyl-D-aspartate (NMDA) receptors in the myenteric plexus mediating NANC relaxation. Sevoflurane inhibited NANC relaxation in a concentration-dependent manner. MK801 concentration-dependently inhibited NANC relaxation, accompanied by a decrease in cGMP production. NMDA induced a concentration-dependent relaxation, which is antagonized by MK801. NMDA stimulated cGMP production, which was inhibited by N^G-nitro-L-arginine. Superoxide dismutase (SOD) shifted the concentration-response relationship of MK801 mediating inhibition of NANC relaxation to the right, but catalase did not. Treatment with diethyldithiocarbamic acid to inactivate Cu/Zn SOD shifted the concentration-response relationships of pyrogallol, ketamine, and MK801 mediating the inhibition of NANC relaxation to the left. These findings suggest that the peripheral NMDA receptors mediates NANC smooth muscle relaxation, and modulates it in part through extracellular production of superoxide anion thus eliminating the relaxant effect of endogenous NO, and that volatile anesthetics may modify LES function by modulating NANC relaxation through antagonism of peripheral NMDA receptors. The functional linkage of peripheral NMDA receptors in the myenteric plexus and NO-cGMP pathway leading to smooth muscle relaxation could provide a key on elucidating the roles of peripheral excitatory amino acids and endogenous NO regulating gastrointestinal motility in the near future.

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  • Analysis of the functional role of the hyperpolarization-activated cation cunert within the centaral nervous system.

    Grant number:15591965  2003 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    FUNAHASHI Makoto, SOGAWA Chiharu, MITOH Yoshihiro, MATSUO Ryuji

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    Grant amount:\3500000 ( Direct expense: \3500000 )

    This study elucidated the membrane properties, morphology and chemosensitivities of area postrema neurons using a patch-clamp technique in rat brain slices. Results are summarized as below.
    1)The activation of the hyperpolarization-activated cation current (I_h) was identified in approximately 62% of area postrema neurons tested. Extracellular Cs^+ ions (2 mM) and ZD7288 (100 mM), a potent selective I_h channel antagonist, blocked I_h and induced a membrane potential hyperpolarization, suggesting the sustained activation of I_h near the resting potential and a contribution from I_h to membrane potential maintenance at more depolarized levels. ZD7288 decreased the spontaneous discharge rate by prolonging the slow depolarization between two spikes, with minimal effect on the amplitude of the afterhyperpolarization or action potential waveforms. I_h stabilized the latency of rebound action potentials. I_h was weakly activated by external 8-bromoadenosine 3,5, cyclic monophosphate (1 mM) or forskolin (50-100 mM), indicating that the I_h channel subtypes in area postrema cells could be modulated by intracellular cAMP. Our findings indicate that I_h contributes to the subthreshold membrane and firing properties of rat area postrema neurons and may regulate their resting membrane potential and firing patterns.
    2)mEPSCs and evoked EPSCs were completely blocked in media containing the non-NMDA ionotropic receptor antagonist, CNQX (10 μM), indicating that EPSC were glutamate events.
    3)Excitatory responses to the bath application of nicotine (50 μM) were found in approximately 78% (35/45) of all cells tested. Responsive cells included both the cells that display the hyperpolarization-activated cation current (Ih) and cells that do not display Ih. An inhibitory effect of nicotine was never seen. We conclude that nicotinic receptors in the rat area postrema can excite cells via (1)a direct post- and/or extrasynaptic mechanism ; and (2)an indirect enhancement of glutamate release.
    4)The bath application of serotonin (5-HT, 50 μM) or phenylbiguanide (PBA, 50 μM, a potent 5-HT_3 receptor agonist) increased the frequency of spontaneous excitatory postsynaptic currents (sEPSCs) or miniature EPSCs (mEPSCs) in 35 of 83 neurons (42%). These increases occurred in all electrophysiological cell classes. No cells exhibited a decrease in EPSC frequency. These results suggest that glutamate release is increased in the area postrema by presynaptic 5-HT_3 receptor activation. Further, we present evidence that 5-HT_3 receptor activation may be able to directly release glutamate from terminals, by-passing a requirement for voltage-dependent calcium entry into terminals. Such a mechanism may contribute to the chemosensitive function of area postrema neurons.
    5)Although propofol suppressed Ih of area postrema neurons in a dose-dependent manner that was similar to what we observed for the hippocampal CA1 neurons, the IC_<50>, for I_h in area postrema neurons (38 mM) was more than six times less than that found for hippocampal CA1 neurons (235 mM). We conclude that rat area postrema neurons are exquisitely sensitive to propofol. Given that reductions of Ih are associated with decreased excitability in neurons, we believe that the known antiemetic effects of propofol anesthesia are at least partly a result of a direct action on area postrema neurons to lower their excitability.

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  • Electrophysiological analysis of impulse formation in the salivary secretory neurons

    Grant number:14370594  2002 - 2003

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    MATSUO Ryuji, MITOH Yoshihiro, FUNAHASHI Makoto, KOBASHI Motoi

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    Grant amount:\9600000 ( Direct expense: \9600000 )

    The superior salivatory (SS) nucleus contains parasympathetic preganglionic neurons innervating the submandibular and sublingual salivary glands and those innervating the anterior part of the tongue. To evaluate mechanisms of impulse formation, we analyzed postsynaptic currents of the SS neurons. The SS neurons were labeled by injection of fluorescent tracers into the peripheral course of the neurons in neonatal Wistar rats. Two days after the injection, the brainstem was sliced and whole-cell patch-clamp recordings were obtained from the labeled neurons. The whole-cell postsynaptic currents were evoked by electrical stimulation applied near the recording cell. The following results were obtained. 1) The SS neurons innervating the different targets scattered and intermingled in the lateral reticular formation of the medulla oblongata, which have 3-4 main dendrites that extended dorso-ventrally about 700 um. 2) The whole-cell currents were antagonized by bicuculline, strychnine, CPP, and CNQX, suggesting that major components of the postsynaptic currents are mediated by GABA_<A->, glycine-, NMDA-, and non-NMDA-receptors. 3) In the SS neurons for the salivary glands, the ratio of the NMDA component to the total excitatory postsynaptic currents was larger than that of the non-NMDA component, and the GABA_A component was larger than the glycine component. 4) Bath application of baclofen, GABA_B-receptor-agonist, reversibly and dose-dependently reduced the amplitude of the inhibitory postsynaptic currents in some neurons. No changes in the input resistance of the postsynaptic membrane were observed during application of baclofen. When paired electrical stimulation (50 ms interval) was applied, the amplitude ratio of the first and second postsynaptic currents was enhanced by baclofen. These results suggest existence of presynaptic GABA_B receptors. These characteristics of the synaptic inputs may be involved in determining the firing properties of the SS neurons.

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  • Electrophysiological analysis of brainstem neurons subserving autonomic and motor oral functions

    Grant number:11470391  1999 - 2000

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B).

    MATSUO Ryuji, MITOH Yoshihiro, FUNAHASHI Makoto, KOBASHI Motoi

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    Grant amount:\14100000 ( Direct expense: \14100000 )

    Neural network in the brainstem subserving autonomic and motor oral functions was investigated by electrophysiological and behavioral studies. In the electrophysiological study, biophysical properties of the superior salivatory (SS) neurons were analyzed by the whole-cell patch clamp technique in sliced brainstem preparations. Neural activity of the trigeminal motor neurons were also analyzed by the intra-cellular recording technique in sliced preparations. The SS neurons innervating the tongue displayed a prominent transient outward current (A-current), and fired up to 70 Hz. Those innervating the salivary glands expressed A-current lasting longer period, and fired up to 30 Hz. The SS neurons innervating the different targets scattered and intermingled in the lateral reticular formation of the medulla oblongata, which have 3-4 main dendrites that extended dorso-ventrally about 700 μm. The two kinds of SS neurons showed similar whole cell currents which of some neurons were antagonized by picrotoxin, 2-amino-5-phosphono-valeric acid (AP-5) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), suggesting that major components of the postsynaptic currents could be mediated by GABA_A-. NMDA- and non-NMDA-receptors. In behavioral study, salivary flow from the submandibular gland and activity of the masseter muscle were chronically recorded from the freely moving rats. During chewing. the amount of saliva and muscle activity were always parallel. When emesis was yielded by intravenous injection so apomorphine, salivary flow rate was correlated with movement of the stomach, but now with jaw movements.
    Through these studies, we conclude that the central nervous system integrates the autonomic and motor oral functions, and it is likely that a contact point between the two functions is situated in the lower brainstem especially the lateral reticular formation. The activity of the pre-motor neurons of the trigeminal motor nucleus should be analyzed in a new research project.

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  • Electrophysiological analysis of the lateral reticular formation controlling orofacial autonomic and motor functions

    Grant number:09470402  1997 - 1998

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    MATSUO Ryuji, MASUDA Yuji, INOUE Tomio, MITHO Yoshihiro, FUNAHASHI Makoto

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    Grant amount:\13200000 ( Direct expense: \13200000 )

    Neural network in the brainstem subserving autonomic and motor oral functions was investigated by anatomical, electrophysiological and behavioral studies. In the anatomical study, FTTC and Rhodamine were injected into the anterior part of the tongue or the secretory nerve of the subamndibular gland, respectively, and the superior salivatory (SS) neurons innervating the tongue and those innervating the submandibular salivary glands were identified in rats. The two groups of SS neurons were similar in shape and intermingled in the lateral reticular formation. In the electrophysiological study, biophysical properties of the SS neurons were analyzed by the whole-cell patch clamp technique in sliced brainstem preparations. The SS neurons innervating the tongue displayed a prominent transient outward current (A-current), and fired up to 70 Hz. Those innervating the salivary glands expressed A current lasting longer period, and fired up to 30 Hz. Post-natal development of the A-currents was observed when we compared between A- currents of one-week and two-week old rats. In behavioral study, salivary flow from the submandibular gland and activity of the masseter muscle were chronically recorded from the freely moving rats, During chewing, the amount of saliva and muscle activity were not always parallel, suggesting different centrifugal effects on autonomic and motor functions.
    Through these studies, we conclude that the autonomic and motor oral functions are under control from the higher central nervous system, but there is a contact point between the two functions in the lower brainstem especially the lateral reticular formation. A further study is needed to clarify relationship between feeding behavior and blood flow rate of the tongue.

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  • 咽頭部および喉頭部の化学受容:Clイオンチャネルの関与

    Grant number:08771607  1996

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    美藤 純弘

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    Grant amount:\1000000 ( Direct expense: \1000000 )

    咽頭部、喉頭部を蒸留水(DW)やNa_2SO_4溶液で刺激すると嚥下が誘発される。NaCl溶液では嚥下が誘発されない。化学刺激による嚥下の誘発に、次のようなメカニズムが考えられた。粘膜の受容細胞にはClイオンチャネルがあり、水刺激では受容細胞の外側のClが少なくなり受容細胞が興奮し、NaCl刺激は受容細胞を抑制する。また、SO_4にはClイオンのチャネル透過性を増加させる作用があり、強い興奮作用を持つ。このように、化学刺激による嚥下にはClイオンチャネルの関与が考えられているが直接の証拠は得られていない。今回、喉頭蓋を支配する上喉頭神経神経から化学刺激による応答を記録し、これらの応答に対する種々Clイオンチャネル阻害剤の効果を調べた。
    ウレタン麻酔下のウサギをガラミンで非動化し、人工呼吸器で呼吸を維持し、喉頭蓋を露出した。喉頭蓋に刺激液を与え、上喉頭神経から応答を記録した。刺激液としてDW、154mM Na_2SO_4、154mM NaCl、及びそれぞれにClイオンチャネル阻害剤(SITS、furosemide、DPC、9-A-C)を0.1mM含有した溶液を使用した。刺激後のリンス溶液は154mM NaCLを用いた。DW、154mM Na_2SO_4の刺激で大きな応答が得られた。しかし、Clイオンチャネル阻害剤でこれらの応答は抑制されなかった。また154mM NaClに対する放電頻度にも影響はなかった以上、今回の実験では種々のClイオンチャネル阻害剤を使用したにもかかわらずそれらの薬物による効果は見られず、化学刺激による嚥下にはClイオンチャネルが関与する直接的証拠は得られなかった。そこで、Clイオンチャネルは受容細胞の表面膜でなく受容細胞間のタイトジャンクションより深い粘膜下の細胞膜にあり、Clイオンはタイトジャンクションを透過できるがClイオンチャネル阻害剤は透過できないので効果がなかったことが考えられる。

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  • Electrophysiological and microdialytic analysis on responses of dopaminergic and noradrenergic neurons in the medulla ablongata (A2aea)

    Grant number:05454502  1993 - 1994

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for General Scientific Research (B)

    ADACHI Akira, MITOH Y, KOBAYASHI M, KOBASHI M

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    Grant amount:\5700000 ( Direct expense: \5700000 )

    Responses of neurons within the nucleus taractus solitarius NTS) to gastric distension were analyzed. Though no primary afferents decrease their discharge rates in response to gastric distension, some NTS neurons decreased their discharage rate resoponding to this stimulus. Convergenece to this neuron of glucose responsive visceral afferent indicates the participation of NTS on control of ingestion.
    Further analysis of the glucose responsive neurons within the area postrema (AP) was made on the brain tissue slice preparations so that confirmed the existence of enteroceptor neuron within this structure.
    It was unable to detect change in dopamine release from the A2 area responding to gastric distension or intraperitoneal injection of LiCl. Futher analysis will be required after technical improvement.
    Homeostatic control of body fluid mediated by the branistem was analyzed by observing effects of portal infusion of hypertonic saline on the neural activity of the dorsal motor nucleus of vagus (DMV)
    The glucose responsive neurons were also found within the DMV.They might play a role on a fail safe mechanism as well as glycemic homeostatic control mediated by the brainstem.

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  • 歯学部先端領域研究センター(ARCOCS)セミナーへの参加

    2015

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    毎月開催される本セミナーに参加し、最先端の研究を学と同時に共同研究の可能性を探った。

 

Class subject in charge

  • Human Physiology (2025academic year) Concentration  - その他

  • Physiology of animal function (2025academic year) 1st semester  - 水1,水2,水3

  • Oral Physiology (2025academic year) Third semester  - 水1~3,木5

  • Practicals: Oral Physiology (2025academic year) special  - その他

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  • Practicals: Oral Physiology (2024academic year) special  - その他

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  • Final digest of diagnosis and treatment of oral disease (2024academic year) special  - その他

  • Final digest of dental sciences (2024academic year) 1st semester  - 火1,火2,火3

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  • Introduction to Dental Science (2023academic year) 1st-4th semester  - その他

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  • Tutorial (2020academic year) 1st semester  - 火4,火5,火6,火7

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Social Activities

  • だ液のはたらき

    Role(s):Lecturer

    大学コンソーシアム岡山  R4年度前期「吉備創生カレッジ」講座  山陽新聞本社ビル  2022.4.16

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    Audience: High school students, College students, Graduate students, General

    Type:Lecture

  • 玉ねぎの切ったときの涙と唾液の分泌の関係について

    Role(s):Advisor

    フジテレビ  林修のニッポンドリル  2020.5

  • たかが唾液、されど唾液〜唾液が出ることの意味〜

    Role(s):Lecturer

    熊本県立宇土高等学校  2018.10.12

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    Audience: High school students

    Type:Visiting lecture

    スーパーサイエンスハイスクールに指定されている同校1・2年生約50人を対象として、唾液の生理学的意義と、その分泌の中枢神経機構について講義を行った。