担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   出版者・発行元：Oxford University Press (OUP)  

Abstract

Establishing an efficient plant regeneration system is a crucial prerequisite for the genetic engineering technology in plants. However, the regeneration rate exhibits considerable variability among genotypes, and the key factors underlying shoot regeneration capacity remain largely elusive. Blueberry leaf explants cultured on a medium rich in cytokinins exhibit direct shoot organogenesis without prominent callus formation, which holds promise for expediting genetic transformation while minimizing somatic mutations during culture. The objective of this study is to unravel the molecular and genetic determinants that govern cultivar-specific shoot regeneration potential in highbush blueberry (Vaccinium corymbosum L.). We conducted comparative transcriptome analysis using two highbush blueberry genotypes, ‘Blue Muffin’ (‘BM’) displaying a high regeneration rate (>80%) and ‘O’Neal’ (‘ON’) exhibiting a low regeneration rate (<10%). The findings revealed differential expression of numerous auxin-related genes, notably, ‘BM’ exhibited higher expression of auxin signaling genes compared to ‘ON’. Among blueberry orthologues of transcription factors involved in meristem formation in Arabidopsis, expressions of VcENHANCER OF SHOOT REGENERATION (VcESR), VcWUSCHEL (VcWUS), and VcCUP-SHAPED COTYLEDON 2.1 (VcCUC2.1) were significantly higher in ‘BM’ relative to ‘ON’. Exogenous application of auxin promoted regeneration, as well as VcESR and VcWUS expressions, whereas the inhibition of auxin biosynthesis yielded the opposite effects. Overexpression of VcESR in ‘BM’ promoted shoot regeneration under phytohormone-free conditions by activating the expression of cytokinin- and auxin-related genes. These findings provide new insights into the molecular mechanisms underlying blueberry regeneration and have practical implications for enhancing plant regeneration and transformation techniques.

DOI： 10.1093/hr/uhae114

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担当区分：筆頭著者   掲載種別：研究論文（国際会議プロシーディングス）   出版者・発行元：International Society for Horticultural Science (ISHS)  

DOI： 10.17660/actahortic.2023.1362.8

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担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japanese Society for Horticultural Science  

DOI： 10.2503/hortj.qh-062

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担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

DOI： 10.1016/j.scienta.2022.111458

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担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   出版者・発行元：Informa UK Limited  

DOI： 10.1080/14620316.2020.1822760

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担当区分：筆頭著者   掲載種別：研究論文（国際会議プロシーディングス）   出版者・発行元：International Society for Horticultural Science (ISHS)  

DOI： 10.17660/actahortic.2020.1280.27

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