Faculty Profiles - KUNISADA Toshiyuki

Papers - KUNISADA Toshiyuki

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液体窒素処理骨による再建を行った肩甲骨Ewing肉腫の1例

金澤智子, 国定俊之, 島村安則, 野田知之, 斉藤太一, 尾崎敏文

肩関節 36 ( 1 ) 167 - 167 2011.9

上腕骨通顆骨折に対する内固定法の力学的比較 ONI plate対従来固定法

島村安則, 今谷潤也, 野田知之, 中原龍一, 齋藤太一, 金澤智子, 井上円加, 小澤正嗣, 古松毅之, 国定俊之, 西田圭一郎, 橋詰博行, 尾崎敏文

日本整形外科学会雑誌 85 ( 8 ) S1338 - S1338 2011.8

脛骨近位部関節内骨折の関節面骨折形態のCTによる評価

小澤正嗣, 野田知之, 金澤智子, 長谷井嬢, 齋藤太一, 中原龍一, 島村安則, 国定俊之, 尾崎敏文

骨折 33 ( Suppl. ) S212 - S212 2011.7

鎖骨骨幹部骨折に対するMIPO法による治療成績

島村安則, 中原龍一, 斉藤太一, 金澤智子, 小澤正嗣, 井上円加, 国定俊之, 野田知之, 尾崎敏文, 島村好信

骨折 33 ( Suppl. ) S239 - S239 2011.7

上腕骨近位端骨折に対する最少侵襲プレート骨接合術(MIPO)による手術成績

島村安則, 中原龍一, 斉藤太一, 金澤智子, 小澤正嗣, 井上円加, 国定俊之, 野田知之, 尾崎敏文, 島村好信

骨折 33 ( Suppl. ) S80 - S80 2011.7

下腿骨幹部骨折術後非感染性偽関節の治療経験

藤原智洋, 野田知之, 金澤智子, 長谷井嬢, 中原龍一, 島村安則, 国定俊之, 尾崎敏文, 寺田忠司

骨折 33 ( Suppl. ) S245 - S245 2011.7

Preclinical evaluation of telomerase-specific oncolytic virotherapy for human bone and soft tissue sarcomas

Tsuyoshi Sasaki, Hiroshi Tazawa, Jo Hasei, Toshiyuki Kunisada, Aki Yoshida, Yuki Morimoto, Yasuo Urata, Kazuhiro Nouso, Toshifumi Ozaki, Toshiyoshi Fujiwara

CANCER RESEARCH 71 2011.4

Preclinical Evaluation of Telomerase-Specific Oncolytic Virotherapy for Human Bone and Soft Tissue Sarcomas Reviewed

Tsuyoshi Sasaki, Hiroshi Tazawa, Jo Hasei, Toshiyuki Kunisada, Aki Yoshida, Yuuri Hashimoto, Shuya Yano, Ryosuke Yoshida, Futoshi Uno, Shunsuke Kagawa, Yuki Morimoto, Yasuo Urata, Toshifumi Ozaki, Toshiyoshi Fujiwara

CLINICAL CANCER RESEARCH 17 ( 7 ) 1828 - 1838 2011.4

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER ASSOC CANCER RESEARCH

Purpose: Tumor-specific replication-selective oncolytic virotherapy is a promising antitumor therapy for induction of cell death in tumor cells but not of normal cells. We previously developed an oncolytic adenovirus, OBP-301, that kills human epithelial malignant cells in a telomerase-dependent manner. Recent evidence suggests that nonepithelial malignant cells, which have low telomerase activity, maintain telomere length through alternative lengthening of telomeres (ALT). However, it remains unclear whether OBP-301 is cytopathic for nonepithelial malignant cells. Here, we evaluated the antitumor effect of OBP-301 on human bone and soft tissue sarcoma cells.
Experimental Design: The cytopathic activity of OBP-301, coxsackie and adenovirus receptor (CAR) expression, and telomerase activity were examined in 10 bone (OST, U2OS, HOS, HuO9, MNNG/HOS, SaOS-2, NOS-2, NOS-10, NDCS-1, and OUMS-27) and in 4 soft tissue (CCS, NMS-2, SYO-1, and NMFH-1) sarcoma cell lines. OBP-301 antitumor effects were assessed using orthotopic tumor xenograft models. The fiber-modified OBP-301 (termed OBP-405) was used to confirm an antitumor effect on OBP-301-resistant sarcomas.
Results: OBP-301 was cytopathic for 12 sarcoma cell lines but not for the non-CAR-expressing OUMS27 and NMFH-1 cells. Sensitivity to OBP-301 was dependent on CAR expression and not on telomerase activity. ALT-type sarcomas were also sensitive to OBP-301 because of upregulation of human telomerase reverse transcriptase (hTERT) mRNA following virus infection. Intratumoral injection of OBP-301 significantly suppressed the growth of OST and SYO-1 tumors. Furthermore, fiber-modified OBP-405 showed antitumor effects on OBP-301-resistant OUMS-27 and NMFH-1 cells.
Conclusions: A telomerase-specific oncolytic adenovirus is a promising antitumor reagent for the treatment of bone and soft tissue sarcomas. Clin Cancer Res; 17(7); 1828-38. (C) 2011 AACR.

DOI： 10.1158/1078-0432.CCR-10-2066

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軟部腫瘍の良悪性鑑別におけるTI-201腫瘍シンチグラフィの有用性

佐藤修平, 稲井良太, 原田聡介, 新家崇義, 郷原英夫, 奥村能啓, 国定俊之, 尾崎敏文, 金澤右

日本医学放射線学会学術集会抄録集 70回 S365 - S366 2011.2

The functional outcome after subtotal scaplectomy for chondrosarcoma: two case reports

OKADA Yoshiki

The Central Japan Journal of Orthopaedic Surgery & Traumatology 54 ( 1 ) 41 - 42 2011

Well leg compartment syndrome following radical cystectomy: A case report

OKADA Yoshiki

The Central Japan Journal of Orthopaedic Surgery & Traumatology 54 ( 4 ) 869 - 870 2011

Minimally invasive plate osteosynthesis for osteofibrous dysplasia of the tibia: a case report. Reviewed International journal

Nakahara H, Kunisada T, Noda T, Ozaki T

Journal of orthopaedic surgery (Hong Kong) 18 ( 3 ) 374 - 377 2010.12

Language：English Publishing type：Research paper (scientific journal)

Osteofibrous dysplasia occurs most frequently in the tibia and may result in deformity and pathological fracture. We report one such case in a 6-year-old girl who underwent minimally invasive plate osteosynthesis using a locking compression plate. The varus deformity of the tibia was manually corrected, and the plate was inserted without extensive surgical exposure. At 20-week follow-up, the fracture had healed completely, and she had returned to normal daily living. At 3-year follow-up, radiographs showed no progression of the varus deformity. Minimally invasive plate osteosynthesis is useful for treatment of pathological fractures caused by osteofibrous dysplasia. It preserves blood flow at the fracture site, leading to good bone healing and stability to prevent further deformity and an early return to daily activity.

DOI： 10.1177/230949901001800325

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ケルブールプレートと人工骨を併用した臼蓋再置換術THAの短期成績の検討

遠藤裕介, 三谷茂, 藤原一夫, 三宅由晃, 鉄永智紀, 国定俊之, 尾崎敏文

日本人工関節学会誌 40 102 - 103 2010.12

Language：Japanese Publisher：(一社)日本人工関節学会

2000年〜2007年に人工骨とケルブールプレート併用の臼蓋側再置換術(THA)を施行した骨欠損症37例中2年以上経過観察できた30例30関節(男性5例、女性25例、44〜78歳・平均66歳)を対象に、短期成績について検討した。前回手術は人工骨頭置換術(BHA)2例、人工股関節置換術(THA)28例で前回手術からの経過観察期間は平均15年(2〜26年)、再置換の理由は無菌性のゆるみ27例、感染2例、脱臼1例、平均追跡期間は4年6ヵ月(2〜9年)であった。術前のAAOS分類はIが5股、IIが2股、IIIが20股、IVが3股であった。術直後のX線像におけるケルブールプレートのフックの設置位置は14股(46%)に外側設置を認め、荷重部の移植骨の厚みは平均15mm(0〜27mm)であった。最終調査時迄に遅発性感染を2例に認め、スクリューのゆるみ・破損を6股(20%)に認めた。カップの内方への移動を7股(23%)に認め移動距離は平均7.2mm(1〜20mm)であった。JOAスコアは術前平均50点から最終観察時平均77点と改善した。感染例2例を除く28股においてカップの移動を生じた5股と問題のなかった23股を比較検討したが両群に術前の平均年齢、BMIに差はなく成績不良群には術前AAOS分類でタイプIVが2股存在した。成績不良群は全例荷重部にブロックを使用せずに人工骨顆粒のみを使用していた。荷重部の移植骨の厚みには有意差は認めず、プレートの設置状態が良好であった15股中2股に成績不良を認めた。

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当科における滑膜肉腫の治療成績

藤原智洋, 森本裕樹, 井谷智, 国定俊之, 尾崎敏文

日本整形外科学会雑誌 84 ( 6 ) S998 - S998 2010.6

Operative Treatment for Pincer Type Femoroacetabular Impingement: A Case Report Reviewed

Hirosuke Endo, Tomoyuki Noda, Shigeru Mitani, Ryuichi Nakahara, Tomonori Tetsunaga, Toshiyuki Kunisada, Toshifumi Ozaki

ACTA MEDICA OKAYAMA 64 ( 2 ) 149 - 154 2010.4

Language：English Publishing type：Research paper (scientific journal) Publisher：OKAYAMA UNIV MED SCHOOL

Femoroacetabular impingement (FAT) is a condition characterized by the impingement of the femur and acetabulum. In Japan, this disorder has become recognized gradually. Here we report a rare case of surgically treated FAT, associated with an osseous protrusion on the acetabulum of a 30-year-old female. Plain computed tomography (CT) and reconstructive 3D-CT images clearly demonstrated an anterolateral bony protrusion. Hip arthroscopy showed no degeneration of the cartilage on either the femoral or acetabular side, but degeneration at the edge of labrum was observed in the region of the bony protrusion. The complete removal of the bony protrusion under hip arthroscopy was thus considered impracticable, and a small skin incision was therefore made anteriorly to approach the acetabulum. The Short-Form 36-Item Health Survey (SF-36) revealed improvement in all scores.

DOI： 10.18926/AMO/32853

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Frequent methylation of RASSF1A in synovial sarcoma and the anti-tumor effects of 5-aza-2'-deoxycytidine against synovial sarcoma cell lines Reviewed

Kunihiko Numoto, Aki Yoshida, Shinsuke Sugihara, Toshiyuki Kunisada, Yuki Morimoto, Yasushi Yoneda, Yasuko Fujita, Keiichiro Nishida, Mamoru Ouchida, Toshifumi Ozaki

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 136 ( 1 ) 17 - 25 2010.1

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

In this study, the methylation status of RASSF1A in synovial sarcomas and the effect of de-methylation on synovial sarcoma were examined.
The methylation status in 74 soft tissue sarcomas (STSs) including 21 synovial sarcomas was determined by methylation specific PCR. The effect of the de-methylating agent 5-aza-2'-deoxycytidine (5-Aza-dC) on synovial sarcoma was examined using synovial sarcoma cell lines (SYO-1 and HS-SY-II).
RASSF1A methylation was observed in 10 (47.6%) of 21 synovial sarcomas and in 10 (18.9%) of 53 the other STSs (P = 0.0295). De-methylation of the cells by treatment with 5-Aza-dC induced re-expression of RASSF1A and growth suppression of the cells. The calculated IC50 of 5-Aza-dC against the SYO-1 and the HS-SY-II cells were 0.9 and 1.3 mu M (96 h), respectively. With twice weekly administration of 1 or 10 mg/kg 5-Aza-dC, the growth of the mouse xenograft tumors of SYO-1 was significantly suppressed in comparison to the controls (P < 0.01).
This is the first report showing the anti-tumor effect of 5-Aza-dC on synovial sarcoma. 5-Aza-dC is suggested to have a good therapeutic potential against synovial sarcoma.

DOI： 10.1007/s00432-009-0632-2

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Giant Cell Tumor of the Sacrum Treated with Selective Intra-Arterial Embolization : A Case Report

Sasaki Tsuyoshi, Morimoto Yuki, Ozaki Toshifumi, Kunisada Toshiyuki, Gobara Hideo

The Journal of the Chugoku-Shikoku Orthopaedic Association 22 ( 2 ) 267 - 272 2010

Language：Japanese Publisher：The Chugoku-Shikoku Orthopaedic Association

Giant cell tumor (GCT) of the sacrum is rare and difficult to treat, although several treatment modalities have been reported. Here we report a patient with sacral GCT who underwent selective intra-arterial embolization.<br>The patient, a 23-year-old woman, had been treated with curettage alone at a local hospital. However, 4 months after surgery, MRI showed recurrence of the tumor, and the patient was referred to our hospital for further treatment.<br>After discussion with her and her family, they accepted intra-arterial embolization of the tumor vessels. Embolization using a temporary embolic agent reduced the size of the tumor and the level of serum acid phosphatase (ACP). However, both the tumor size and the level of ACP increased a few months after the procedure. Therefore, embolization was performed using an embolic agent consisting of spherical particles. MRI showed a marked reduction of tumor size 1 month after the last embolization, and the level of serum ACP also fell to less than the standard value.<br>Embolization using a spherical-particulate embolic agent can be an effective treatment for sacral GCT.

DOI： 10.11360/jcsoa.22.267

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Short-term follow-up of hip transposition using external fixator after resection of pelvic tumor

FUJIWARA Tomohiro

The Central Japan Journal of Orthopaedic Surgery & Traumatology 53 ( 4 ) 937 - 938 2010

自然退縮を認めた骨軟骨腫の2例

藤原智洋, 国定俊之, 井谷智, 森本裕樹, 尾崎敏文

岡山医学会雑誌 121 ( 3 ) 233 - 233 2009.12

血管病変と診断され血管置換術が行われた滑膜肉腫の2例

藤原智洋, 国定俊之, 佐々木剛, 井谷智, 森本裕樹, 尾崎敏文

中部日本整形外科災害外科学会雑誌 52 ( 秋季学会 ) 214 - 214 2009.9

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