Updated on 2024/06/14

写真a

 
FUKADA Fumi
 
Organization
Institute of Plant Science and Resources Special-Appointment Assistant Professor
Position
Special-Appointment Assistant Professor
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Degree

  • 博士(農学) ( 2017.3   京都府立大学 )

Research Areas

  • Life Science / Plant molecular biology and physiology

  • Environmental Science/Agriculture Science / Plant protection science

  • Life Science / Applied microbiology

Education

  • Kyoto Prefectural University   生命環境科学研究科  

    2012.4 - 2017.3

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  • Kyoto Prefectural University   生命環境学部  

    2008.4 - 2012.3

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Research History

  • Okayama University   Institute of Plant Science and Resources   WTT assistant professor

    2021.4

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  • Okayama University   Institute of Plant Science and Resources

    2020.10 - 2021.3

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  • Max Planck Institute for Terrestrial Microbiology   Organismic Interactions   Postdoctoral Fellow

    2017.4 - 2020.9

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    Country:Germany

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  • Kyoto Prefectural University   Graduate School of Life and Environmental Sciences (MC and DC) Division of Applied Life Sciences

    2014.4 - 2017.3

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Professional Memberships

Committee Memberships

  • 一般社団法人 日本植物生理学会   編集委員補  

    2023.1   

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  • 糸状菌分子生物学研究会若手の会   運営委員  

    2021.11   

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  • 一般社団法人 日本植物病理学会   編集幹事  

    2021.1 - 2023.3   

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    Committee type:Academic society

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Papers

  • An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and function. Reviewed International journal

    Yuying Li, Qiong Wang, Huimin Jia, Kazuya Ishikawa, Ken-Ichi Kosami, Takahiro Ueba, Atsumi Tsujimoto, Miki Yamanaka, Yasuyuki Yabumoto, Daisuke Miki, Eriko Sasaki, Yoichiro Fukao, Masayuki Fujiwara, Takako Kaneko-Kawano, Li Tan, Chojiro Kojima, Rod A Wing, Alfino Sebastian, Hideki Nishimura, Fumi Fukada, Qingfeng Niu, Motoki Shimizu, Kentaro Yoshida, Ryohei Terauchi, Ko Shimamoto, Yoji Kawano

    Nature communications   15 ( 1 )   4610 - 4610   2024.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    NLR family proteins act as intracellular receptors. Gene duplication amplifies the number of NLR genes, and subsequent mutations occasionally provide modifications to the second gene that benefits immunity. However, evolutionary processes after gene duplication and functional relationships between duplicated NLRs remain largely unclear. Here, we report that the rice NLR protein Pit1 is associated with its paralogue Pit2. The two are required for the resistance to rice blast fungus but have different functions: Pit1 induces cell death, while Pit2 competitively suppresses Pit1-mediated cell death. During evolution, the suppression of Pit1 by Pit2 was probably generated through positive selection on two fate-determining residues in the NB-ARC domain of Pit2, which account for functional differences between Pit1 and Pit2. Consequently, Pit2 lost its plasma membrane localization but acquired a new function to interfere with Pit1 in the cytosol. These findings illuminate the evolutionary trajectory of tandemly duplicated NLR genes after gene duplication.

    DOI: 10.1038/s41467-024-48943-5

    PubMed

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  • Mitigating the Trade-Off between Growth and Stress Resistance in Plants by Fungal Volatile Compounds

    Fumi Fukada

    Plant And Cell Physiology   2024.2

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcae005

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  • Niemann-Pick Type C Proteins Are Required for Sterol Transport and Appressorium-Mediated Plant Penetration of Colletotrichum orbiculare. Reviewed International journal

    Sayo Kodama, Naoki Kajikawa, Fumi Fukada, Yasuyuki Kubo

    mBio   13 ( 5 )   e0223622   2022.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    Many biotrophic and hemibiotrophic fungal pathogens use appressoria to directly penetrate the host plant surface. In the cucumber anthracnose fungus Colletotrichum orbiculare, differentiation of appressoria requires a proper G1/S cell cycle progression, regulated by the GTPase-activating protein complex CoBub2-CoBfa1 and its downstream GTPase CoTem1. To explore the mechanisms by which the CoTem1 cascade regulates plant infection, we screened for CoTem1 interaction factors and identified a Niemann-Pick type C2 homolog (CoNpc2). Niemann-Pick type C proteins NPC1 and NPC2 are sterol-binding proteins required for sterol export from lysosomes (vacuoles) in humans and yeasts. We showed that CoNpc2 colocalized with CoNpc1 in late endosomes and vacuoles and that disruption of its gene resulted in aberrant sterol accumulation in vacuoles and loss of sterol membrane localization, indicating that NPC proteins are engaged in sterol transport in C. orbiculare. For appressorium infection, sterol transport and proper distribution mediated by CoNpc1 and CoNpc2 are critical for membrane integrity and membrane curvature with actin assembly, leading to penetration peg emergence and appressorial cone formation. Our results revealed a novel mechanism by which NPC proteins regulate appressorium-mediated plant infection. IMPORTANCE Fungal morphogenesis requires accurate cell cycle progression. Two-component GTPase-activating protein (GAP) CoBub2-CoBfa1 interacts with downstream GTPase CoTem1 and is required for G1/S progression to establish plant infection in Colletotrichum orbiculare. To understand the pathogenicity related functions of CoTem1 downstream, we identified a Niemann-Pick type C2 homolog (CoNpc2) as a novel physical interaction factor with CoTem1. Whereas NPC proteins (NPC1 and NPC2) are essential for sterol homeostasis in humans and yeasts, their functions in plant invasion by pathogenic fungi have remained unclear. In this study, we show that CoNPC1 and CoNPC2 play a critical role in intracellular sterol transport and that appropriate sterol distribution is required for membrane integrity and membrane curvature with actin assembly that leads to appressorium-mediated plant penetration and pathogenicity of C. orbiculare. Our findings suggest the importance of sterol distribution in fungal morphogenesis during plant infection.

    DOI: 10.1128/mbio.02236-22

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  • The Small GTPase OsRac1 Forms Two Distinct Immune Receptor Complexes Containing the PRR OsCERK1 and the NLR Pit Reviewed

    Akira Akamatsu, Masayuki Fujiwara, Satoshi Hamada, Megumi Wakabayashi, Ai Yao, Qiong Wang, Ken-ichi Kosami, Thu Thi Dang, Takako Kaneko-Kawano, Fumi Fukada, Ko Shimamoto, Yoji Kawano

    Plant and Cell Physiology   62 ( 11 )   1662 - 1675   2021.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    <title>Abstract</title>
    Plants employ two different types of immune receptors, cell surface pattern recognition receptors (PRRs) and intracellular nucleotide-binding and leucine-rich repeat-containing proteins (NLRs), to cope with pathogen invasion. Both immune receptors often share similar downstream components and responses but it remains unknown whether a PRR and an NLR assemble into the same protein complex or two distinct receptor complexes. We have previously found that the small GTPase OsRac1 plays key roles in the signaling of OsCERK1, a PRR for fungal chitin, and of Pit, an NLR for rice blast fungus, and associates directly and indirectly with both of these immune receptors. In this study, using biochemical and bioimaging approaches, we revealed that OsRac1 formed two distinct receptor complexes with OsCERK1 and with Pit. Supporting this result, OsCERK1 and Pit utilized different transport systems for anchorage to the plasma membrane (PM). Activation of OsCERK1 and Pit led to OsRac1 activation and, concomitantly, OsRac1 shifted from a small to a large protein complex fraction. We also found that the chaperone Hsp90 contributed to the proper transport of Pit to the PM and the immune induction of Pit. These findings illuminate how the PRR OsCERK1 and the NLR Pit orchestrate rice immunity through the small GTPase OsRac1.

    DOI: 10.1093/pcp/pcab121

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    Other Link: https://academic.oup.com/pcp/article-pdf/62/11/1662/42269784/pcab121.pdf

  • NB-LRR-encoding genes conferring susceptibility to organophosphate pesticides in sorghum. Reviewed International journal

    Zihuan Jing, Fiona Wacera W, Tsuneaki Takami, Hideki Takanashi, Fumi Fukada, Yoji Kawano, Hiromi Kajiya-Kanegae, Hiroyoshi Iwata, Nobuhiro Tsutsumi, Wataru Sakamoto

    Scientific reports   11 ( 1 )   19828 - 19828   2021.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    Organophosphate is the commonly used pesticide to control pest outbreak, such as those by aphids in many crops. Despite its wide use, however, necrotic lesion and/or cell death following the application of organophosphate pesticides has been reported to occur in several species. To understand this phenomenon, called organophosphate pesticide sensitivity (OPS) in sorghum, we conducted QTL analysis in a recombinant inbred line derived from the Japanese cultivar NOG, which exhibits OPS. Mapping OPS in this population identified a prominent QTL on chromosome 5, which corresponded to Organophosphate-Sensitive Reaction (OSR) reported previously in other mapping populations. The OSR locus included a cluster of three genes potentially encoding nucleotide-binding leucine-rich repeat (NB-LRR, NLR) proteins, among which NLR-C was considered to be responsible for OPS in a dominant fashion. NLR-C was functional in NOG, whereas the other resistant parent, BTx623, had a null mutation caused by the deletion of promoter sequences. Our finding of OSR as a dominant trait is important not only in understanding the diversified role of NB-LRR proteins in cereals but also in securing sorghum breeding free from OPS.

    DOI: 10.1038/s41598-021-98908-7

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  • A small Ustilago maydis effector acts as a novel adhesin for hyphal aggregation in plant tumors Reviewed

    Fumi Fukada, Nicole Rössel, Karin Münch, Timo Glatter, Regine Kahmann

    New Phytologist   231 ( 1 )   416 - 431   2021.7

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/nph.17389

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.17389

  • Plant pathogenic fungi Colletotrichum and Magnaporthe share a common G1 phase monitoring strategy for proper appressorium development Reviewed International journal

    Fumi Fukada, Sayo Kodama, Takumi Nishiuchi, Naoki Kajikawa, Yasuyuki Kubo

    New Phytologist   222 ( 4 )   1909 - 1923   2019.6

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    To breach the plant cuticle, many plant pathogenic fungi differentiate specialized infection structures (appressoria). In Colletotrichum orbiculare (cucumber anthracnose fungus), this differentiation requires unique proper G1 /S phase progression, regulated by two-component GTPase activating protein CoBub2/CoBfa1 and GTPase CoTem1. Since their homologues regulate mitotic exit, cytokinesis, or septum formation from yeasts to mammals, we asked whether the BUB2 function in G1 /S progression is specific to plant pathogenic fungi. Colletotrichum higginsianum and Magnaporthe oryzae were genetically analyzed to investigate conservation of BUB2 roles in cell cycle regulation, septum formation, and virulence. Expression profile of cobub2Δ was analyzed using a custom microarray. In bub2 mutants of both fungi, S phase initiation was earlier, and septum formation coordinated with a septation initiation network protein and contractile actin ring was impaired. Earlier G1 /S transition in cobub2Δ results in especially high expression of DNA replication genes and differing regulation of virulence-associated genes that encode proteins such as carbohydrate-active enzymes and small secreted proteins. The virulence of chbub2Δ and mobub2Δ was significantly reduced. Our evidence shows that BUB2 regulation of G1 /S transition and septum formation supports its specific requirement for appressorium development in plant pathogenic fungi.

    DOI: 10.1111/nph.15728

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.15728

  • Neofunctionalization of the secreted Tin2 effector in the fungal pathogen Ustilago maydis International journal

    Shigeyuki Tanaka, Gabriel Schweizer, Nicole Rössel, Fumi Fukada, Marco Thines, Regine Kahmann

    Nature Microbiology   4 ( 2 )   251 - 257   2019.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media {LLC}  

    DOI: 10.1038/s41564-018-0304-6

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    Other Link: http://www.nature.com/articles/s41564-018-0304-6.pdf

  • Development of the infection strategy of the hemibiotrophic plant pathogen, Colletotrichum orbiculare, and plant immunity Reviewed

    Yasuyuki Kubo, Ken Harata, Sayo Kodama, Fumi Fukada

    Physiological and Molecular Plant Pathology   95   32 - 36   2016.7

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier {BV}  

    DOI: 10.1016/j.pmpp.2016.02.008

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  • Colletotrichum orbiculare Regulates Cell Cycle G1/S Progression via a Two-Component GAP and a GTPase to Establish Plant Infection Reviewed

    Fumi Fukada, Yasuyuki Kubo

    The Plant Cell   27 ( 9 )   2530 - 2544   2015.9

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:American Society of Plant Biologists ({ASPB})  

    DOI: 10.1105/tpc.15.00179

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MISC

  • Functional analysis on RALF family peptide that regulates rice immunity

    深田史美, TING Guo, WANG Wanqing, 西村秀希, 小野奈津子, 水口洋平, 豊田敦, 古田智敬, 河野洋治

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • トウモロコシ黒穂病菌が分泌するエフェクター Lep1 は植物腫瘍組織中で新規アドへシンとして機能する

    第62回 日本植物生理学会年会 プログラム集   39 - 39   2021

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  • トウモロコシ黒穂病菌は植物腫瘍において菌糸接着を促すためにエフェクターを新規のアドへシンとして利用する

    深田史美, Nicole Rössel, Karin Münch, Timo Glatter, Regine Kahmann

    令和3年度 日本植物病理学会大会プログラム・講演要旨予稿集   41 - 41   2021

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  • The virulence factors CoNpc1 and CoNpc2 are involved in intracellular sterol transport and penetration peg formation of Colletotrichum orbiculare.

    小玉紗代, 梶河直起, 深田史美, 久保康之

    日本植物病理学会報   86 ( 1 )   2020

  • ウリ類炭疽病菌のCoNPC2は出芽酵母におけるステロール輸送因子NPC2の機能的オルソログであり病原性に関与する

    梶河直起, 深田史美, 久保康之

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • 炭疽病菌およびいもち病菌においてRabGAP Bub2は細胞周期および隔壁形成を制御し植物感染に関与する

    深田史美, 西内巧, 久保康之

    日本植物病理学会報   83 ( 1 )   2017

  • ウリ類炭疽病菌におけるCoPpt1は非選択的オートファジー関連因子CoAtg8と共局在し病原性に関与する

    梶河直起, 深田史美, 久保康之

    日本植物病理学会大会プログラム・講演要旨予稿集   2017   2017

  • 酵母ツーハイブリッド法を用いたウリ類炭疽病菌における細胞周期制御因子CoTem1相互作用因子の同定

    梶河直起, 深田史美, 久保康之

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • ウリ類炭疽病菌のLacO/LacI-GFPシステムを用いた細胞周期およびCoTem1局在解析

    深田史美, 久保康之

    日本植物病理学会報   82 ( 1 )   2016

  • ウリ類炭疽病菌の感染器官の形態形成と病原性研究

    久保康之, 西内巧, 原田賢, 原田賢, 小玉紗代, 深田史美

    日本植物病理学会植物感染生理談話会論文集   ( 51 )   2016

  • ウリ類炭疽病菌におけるCoBub2-CoBfa1と出芽酵母Bub2-Bfa1のスワッピングはG1/S期進行を遅延させる

    深田史美, 坂口歩, 久保康之

    日本植物病理学会報   81 ( 1 )   2015

  • ウリ類炭疽病菌はRab GAPおよびGTPaseを介してG1/S期進行を制御し植物感染を成立させる

    深田史美, 久保康之

    日本生化学会大会(Web)   88th   2015

  • ウリ類炭疽病菌GAP複合体CoBub2/CoBfa1はGTPase CoTem1を介してG1/S期進行制御および病原性に関与する

    深田史美, 坂口歩, 久保康之

    日本植物病理学会大会プログラム・講演要旨予稿集   2015   2015

  • ウリ類炭疽病菌のCoBub2/CoBfa1は付着器分化過程におけるG1/S期の進行制御および病原性に関与する

    深田史美, 坂口歩, 久保康之

    日本植物病理学会報   80 ( 1 )   2014

  • ウリ類炭疽病菌の選択的なオートファジー因子CoATG20は宿主への侵入に必要である

    DONG C., 深田史美, 原田賢, 小玉紗代, 坂口歩, 久保康之

    日本植物病理学会報   80 ( 1 )   2014

  • プロテインホスファターゼ2A制御サブユニットをコードする出芽酵母のRTS1のホモログCoRTS1はウリ類炭疽病菌において病原性,適切な胞子形成に必要である

    川端昂, 深田史美, 坂口歩, 久保康之

    日本植物病理学会報   80 ( 1 )   2014

  • ウリ類炭疽病菌における出芽酵母BUB2のホモログ遺伝子CoBUB2は付着器分化過程における適切な細胞周期の進行に関与する

    深田史美, 坂口歩, 久保康之

    日本植物病理学会報   79 ( 1 )   2013

  • ウリ類炭疽病菌の出芽酵母BUB2ホモログ遺伝子CoBUB2は形態形成および病原性に関与する

    深田史美, 坂口歩, 久保康之

    日本植物病理学会大会プログラム・講演要旨予稿集   2012   2012

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Presentations

  • 植物-病原糸状菌相互作用における分泌タンパク質・ペプチドの解析 Invited

    深田史美, Ting Guo, Pingyu Wang, 河野洋治

    令和4年度植物感染生理談話会  2022.9.6 

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    Event date: 2022.9.5 - 2022.9.7

    Presentation type:Oral presentation (invited, special)  

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  • A secreted RALF peptide modulates rice immune response upon Magnaporthe oryzae infection Invited

    Fukada, F, Guo, T, Wang, W, Nishimura, H, Ono, N, Minakuchi, Y, Toyoda, A, Kawano, Y

    12th Japan-US Seminar in Plant Pathology  2022.8.30 

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    Event date: 2022.8.28 - 2022.9.2

    Presentation type:Oral presentation (invited, special)  

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  • イネ免疫を制御するRALFファミリーペプチドの機能解析

    深田史美, Ting Guo, Wanqing Wang, 西村秀希, 小野奈津子, 水口洋平, 豊田敦, 古田智敬, 河野洋治

    令和4年度日本植物病理学会大会  2022.3.28 

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    Event date: 2022.3.27 - 2022.3.30

    Presentation type:Oral presentation (general)  

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  • A secreted virulence effector protein of the biotrophic fungal plant pathogen Ustilago maydis Invited

    深田史美, Nicole Rössel, Timo Glatter, Karin Münch, Petra Happel, Regine Kahmann

    第96回 日本細菌学会  2023.3.18 

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  • Secreted proteins and peptides used for the interaction between plant cell and plant pathogenic fungi Invited

    深田史美

    神戸大学第57回インターゲノミクスセミナー  2022.11.11 

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  • 植物サイトカインRALFaによる免疫制御と病原菌との相互作用 Invited

    深田史美

    岩手生工研所内セミナー  2022.9.9 

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  • 植物腫瘍内で黒穂病菌の増殖を促進するエフェクター Invited

    第41回 日本植物病理学会 関西部会 若手の会  2021.9.17 

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  • Ustilago maydis secretes the novel virulence-promoting Lep1 effector for hyphal aggregation in plant tumors

    Fumi Fukada, Nicole Rössel, Karin Münch, Timo Glatter, Regine Kahmann

    2021 IS-MPMI Congress: eSymposia Series  2021.9.15 

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  • Ustilago maydis utilizes an effector as a novel adhesin to promote hyphal aggregation in plant tumors

    Fukada Fumi, Nicole Rössel, Glatter Timo, Kahmann Regine

    2021.3.17 

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  • A secreted Ustilago maydis effector acts as a novel adhesin for hyphal aggregation in plant tumors

    Fumi Fukada, Nicole Rössel, Timo Glatter, Karin Münch, Petra Happel, Regine Kahmann

    2021.3.14 

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  • A small Ustilago maydis effector acts as a novel adhesin for hyphal aggregation in plant tumors

    Fumi Fukada, Nicole Rössel, Timo Glatter, Regine Kahmann

    2020.11.13 

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  • A secreted small protein of Ustilago maydis is used as a mediator of hydrophobicity for hyphal aggregates formation after plant colonization Invited

    2020 Japan-US Early Career Online Symposium  2020.9.29 

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Awards

  • 学術奨励賞

    2024.3   日本植物病理学会  

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  • 育志賞

    2017.3   日本学術振興会  

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Research Projects

  • 植物由来の細胞外小胞による病原菌感染に対するカウンターアタックの解析

    Grant number:24K17890  2024.04 - 2027.03

    日本学術振興会  科学研究費助成事業  若手研究

    深田 史美

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    Grant amount:\4680000 ( Direct expense: \3600000 、 Indirect expense:\1080000 )

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  • Deciphering activation and evolutionary mechanisms of the paired NLR immune receptors based on paralog suppression and neofunctionalization

    Grant number:23H02213  2023.04 - 2027.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    河野 洋治, 深田 史美

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    Grant amount:\18590000 ( Direct expense: \14300000 、 Indirect expense:\4290000 )

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  • 植物の免疫シグナル因子を逆手に取った病原菌の宿主認識機構

    Grant number:JPMJPR22D8  2022.10 - 2026.03

    国立研究開発法人科学技術振興機構  さきがけ 

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    Authorship:Principal investigator 

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  • 植物サイトカインによる免疫システムの解明

    2021.04 - 2024.03

    文部科学省  科学研究費助成事業 若手研究  若手研究

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    Authorship:Principal investigator 

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  • 植物病原糸状菌の侵入器官の形態形成と細胞周期の制御機構に関する研究

    Grant number:14J11463  2014.04 - 2017.03

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    深田 史美

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    Grant amount:\3200000 ( Direct expense: \3200000 )

    一般に糸状菌の形態形成は適切な細胞周期進行に基づいて行われるが、植物病原糸状菌において細胞周期制御と感染過程における形態分化の関連性に関する研究事例は極めて限られている。申請者はこれまでに、ウリ科植物を宿主とするウリ類炭疽病菌の植物への病原性にはRab GAP複合体を構成するBub2/Bfa1によるG1/S期の移行抑制が必要であることを明らかにしてきた。一方で、酵母や非植物病原糸状菌におけるこれらの相同遺伝子はG1/S期の進行制御には関与せずM期の進行制御や隔壁形成に関与することから、本シグナルカスケードは生物種によって異なる機能を持つ可能性が示唆される。本年度は、第一にマイクロアレイを用いたトランスクリプトーム解析により、GAP複合体制御下の遺伝子発現変動を検討した。その結果、bub2破壊株ではDNA合成酵素やDNAヘリカーゼといったDNA複製に関与するタンパク質をコードする遺伝子群の顕著な発現上昇が認められた。加えて転写因子、小型分泌タンパク質、糖質関連酵素、膜輸送体に関連する遺伝子に関しても大幅な発現変動が認められ、これらの遺伝子発現の変動が病原性低下の要因となる可能性が強く示唆された。また、ウリ類炭疽病菌と同様に付着器と呼ばれる侵入器官を介した植物感染を行う植物病原菌におけるGAP複合体の機能の保存性を検討した。その結果、アブラナ科炭疽病菌、イネいもち病菌におけるbub2破壊株はG1/S期が異常に早期化した上に、核分裂に伴う隔壁形成の頻度が顕著に低下し、宿主植物への侵入能力が顕著に低下した。以上の結果は、本GAP複合体による制御機構が炭疽病菌といもち病菌に共通して存在し機能することを示しており、付着器を介した植物感染を行う植物病原糸状菌が独自の細胞周期、隔壁形成制御機構を獲得してきた可能性を示唆する。

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