Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

In biofluids, charged functional groups on the surface of nanoparticles (NPs) interact with cells through the protein corona. However, the cascade effects of charged groups on corona formation and cellular uptake remain unclear. Herein, carboxy, sulfate, and amino groups are quantitatively introduced at the periphery of polyglycerol (PG)-grafted nanodiamond and superparamagnetic iron oxide NP to probe their roles in corona formation and cellular uptake. The uptake efficiency and intracellular aggregation state of NPs are revealed to correlate with protein affinity of the charged groups; sulfate at lower density and carboxylate exhibit no affinity to proteins, inducing negligible or no cellular uptake. In contrast, sulfate at higher density and ammonium associate with fetal bovine serum proteins to alter the aggregation state of the internalized NPs. It is further demonstrated that the distinct protein corona profiles on NP-PG-OSO3- and NP-PG-NH3+ surfaces dictate their uptake mechanism. The protein corona of NP-PG-OSO3- suppresses cellular uptake via downregulation of macropinocytosis and clathrin-mediated endocytosis, whereas that of NP-PG-NH3+ enhances uptake through upregulation of macropinocytosis and caveolae-mediated endocytosis. This study clarifies the elusive role of the charged groups in protein adsorption and cellular uptake, which sheds light on NP design for controlled cellular uptake and theranostics in nanomedicine.

DOI： 10.1002/adfm.202111077

Web of Science

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/adfm.202111077

Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

This on-chip device realizes large-area and predictable spin detection of fluorescent nanodiamonds, providing a scalable platform for quantum-sensor-based bioassay.

DOI： 10.1039/d2lc00112h

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Chemical Society of Japan  

DOI： 10.1246/bcsj.20210200

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

DOI： 10.1021/acs.molpharmaceut.1c00188

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1002/cnma.202000271

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cnma.202000271

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.carbon.2020.02.089

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

Upon contact with biofluids, proteins are quickly adsorbed onto the nanoparticle (NP) surface to form a protein corona, which initiates the opsonization and facilitates the rapid clearance of the NP by macrophage uptake. Although polyethylene glycol (PEG) functionalization has been the standard approach to evade macrophage uptake by reducing protein adsorption, it cannot fully eliminate nonspecific uptake. Herein, polyglycerol (PG) grafting is demonstrated as a better alternative to PEG. NPs of various size and material were grafted with PG and PEG at 30, 20, and 10 wt % contents by controlling the reaction conditions, and the resulting NP-PG and NP-PEG were characterized qualitatively by IR spectroscopy and quantitatively by thermogravimetric analysis. Their resistivity to adsorption of the proteins in fetal bovine serum and human plasma were compared by polyacrylamide gel electrophoresis, bicinchoninic acid assay, and liquid chromatography-tandem mass spectrometry, giving a consistent conclusion that PG shields protein adsorption more efficiently than does PEG. The macrophage uptake was assayed by transmission electron microscopy and by extinction spectroscopy or inductively coupled plasma mass spectrometry, revealing that PG avoids macrophage uptake more efficiently than does PEG. In particular, a NP coated with PG at 30 wt % (NP-PG-h) prevents corona formation almost completely, regardless of NP size and core material, leading to the complete evasion of macrophage uptake. Our findings demonstrate that PG grafting is a promising strategy in nanomedicine to improve anti-biofouling property and stealth efficiency in nanoformulations.

DOI： 10.1021/acsnano.0c02289

PubMed

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Preferential accumulation of nanoparticles in a tumor is realized commonly by combined effects of active and passive targeting. However, passive targeting based on an enhanced permeation and retention (EPR) effect is not sufficient to observe clear tumor fluorescence images in most of the in vivo experiments using tumor-bearing mice. Herein, polyglycerol-functionalized nanodiamonds (ND-PG) conjugated with cyanine dye (Cy7) are synthesized and it is found that the resulting ND-PG-Cy7 is preferentially accumulated in the tumor, giving clear fluorescence in in vivo and ex vivo fluorescence images. One of the plausible reasons is the longer in vivo blood circulation time of ND-PG-Cy7 (half-life: 58 h determined by the pharmacokinetic analysis) than that of other nanoparticles (half-life: <20 h in most of the previous reports). In a typical example, the fluorescence intensity of tumors increases due to continuous tumor accumulation of ND-PG-Cy7, even more than one week postinjection. This may be owing to the stealth effect of PG that was reported previously, avoiding recognition and excretion by reticuloendothelial cells, which are abundant in liver and spleen. In fact, the fluorescence intensities from the liver and spleen is similar to those from other organs, while the tumor exhibits much stronger fluorescence in the ex vivo image.

DOI： 10.1002/smll.201901930

Scopus

PubMed

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/smll.201901930

Language：English   Publishing type：Research paper (scientific journal)   Publisher：IOP Publishing  

Cancer phototherapy (PT) including photodynamic therapy (PDT) and photothermal therapy (PTT) has attracted extensive interest due to its non-invasive and region-specific nature. In order to enhance the efficacy, we have fabricated various nanocomposites consisting of a photosensitizer (chlorin e 6 (Ce6), pheophorbide a (Pa) or bacteriopheophorbide a (bPa)) for PDT and a two-dimensional nanosheet (graphene or MoS2) for PTT by a one-pot protocol through liquid phase exfoliation. In addition, an anticancer drug (doxorubicin (DOX) or irinotecan (Ir)) was loaded on the nanosheet in the same way for comparison. The nanocomposites demonstrated stability when dispersed in water except for MoS2-Ir. We observed that MoS2-Ce6 killed cancer cells over ten times more effectively than other Ce6-loaded nanocomposites.

DOI： 10.1088/2053-1583/ab377b

Scopus

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Other Link： https://iopscience.iop.org/article/10.1088/2053-1583/ab377b/pdf

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.toxlet.2018.11.002

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

DOI： 10.1007/s11356-018-3111-y

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Other Link： http://link.springer.com/content/pdf/10.1007/s11356-018-3111-y.pdf

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

DOI： 10.1093/nar/gky707

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

DOI： 10.1007/s11356-017-9626-9

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Other Link： http://link.springer.com/content/pdf/10.1007/s11356-017-9626-9.pdf

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.envpol.2016.03.022

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1002/smll.200902049

CiNii Article

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