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Association Analysis between the Deletion Mutant Allele of Claudin‐16 Deficiency and Carcass Traits in Japanese Black Cattle.

小林直彦, 平野貴, 揖斐隆之, 大谷健, 杉本喜憲

Animal Science Journal 73 ( 1 ) 19 - 23 2002.2

Language：Japanese Publisher：公益社団法人日本畜産学会

Claudin-16 (CL-16) deficiency is an autosomal recessive disease in Japanese black cattle. The association analysis between the deletion mutant allele (d) of CL-16 deficiency and carcass traits was investigated. Normal allele (D) and mutant allele (d) were diagnosed in 464 fattened progeny of three carrier sires (Dd), and 140 cows of Sire 1 whose common ancestor was revealed as Dd. The progeny were composed of 230 normal (DD) and 234 carrier (Dd). The cows were composed of 60 DD and 80 Dd. The association analysis was carried out among the CL-16 genotypes, and carcass traits and predicted breeding value for carcass traits using the 464 progeny and 140 cows, respectively. Analysis of variance was performed considering the CL-16 genotypes, sire, sex, farm and age (linear and quadratic) as the source of variance in order to evaluate factors affecting carcass traits. Significant differences in BMS number, carcass weight, area of rib eye, and rib thickness were observed between the sires. Significant differences in carcass weight were observed in the influence of sex. Significant differences in BMS number, carcass weight, and rib thickness were observed between farms. The regression of any carcass traits on final age (linear and quadratic) was not statistically significant. The significant association of the CL-16 genotypes were not seen in the carcass traits after the effects of environmental factors were eliminated. Moreover, a significant association of the genotypes was not seen (P>0.05) with predicted breeding value for carcass traits in cows of Sire-1. Furthermore, these carcass traits had no significant linkage with the approximately 30 cM region including CL-16 locus in a half-sib family of Sire-1 comprising 136 offspring (P>0.05). Therefore, exclusion of the mutant allele d from Japanese Black cattle population would not deteriorate the carcass traits.

DOI： 10.2508/chikusan.73.19

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Differential display method used for the comparison of mRNA expression level among many samples

NAGATA Yukihiro, IBI Takayuki, TANIGUCHI Yukio, YAMADA Takahisa, SASAKI Yoshiyuki

The journal of animal genetics 30 ( 1 ) 11 - 18 2002

Language：Japanese Publisher：Japanese Society of Animal Breeding and Genetics

A reproducibility of experimental data is important for application of differential display method to the comparison of mRNA expression level among many samples. First, we developed the optimum method to evaluate size (BP) and yield (IOD) of PCR products by analyzing an electrophoretic image. BP (A), which is a way to calculate size of each product by using 3 molecular markers loaded in extreme and central lanes of gel as references, was optimal for size evaluation. Further, IOD (A) and IOD (P), respectively, which are way to use a measured absolute value of signal intensity of each product, and to use a relative value of signal intensity of each product as proportion of total sum of all products in an identical lane, as yield, were determined as the suboptimum and optimum evaluation methods. Next, we performed an analysis of variance of intergel, interlane, and interreaction effects, when using the optimum method. We detected statistically significant effects for intergel and interlane, but not for interreaction, when using BP (A) method. As for IOD (A) and IOD (P), significant effects for intergel and interreaction were observed, but not for interlane. Finally, we evaluated the replicate pattern that is suitable for a largescale gene expression profiling, by examining coefficient of variation of combined effects for BP and IOD obtained by 4 different replicate patterns.

DOI： 10.5924/abgri2000.30.11

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Association Analysis between the Deletion Mutant Allele of Claudin-16 Deficiency and Carcass Traits in Japanese Black Cattle

KOBAYASHI Naohiko, HIRANO Takashi, IBI Takayuki, OHTANI Tuyoshi, SUGIMOTO Yoshikazu

Nihon Chikusan Gakkaiho 73 ( 1 ) 19 - 23 2002

Language：Japanese Publisher：Japanese Society of Animal Science

Claudin-16 (CL-16) deficiency is an autosomal recessive disease in Japanese black cattle. The association analysis between the deletion mutant allele (d) of CL-16 deficiency and carcass traits was investigated. Normal allele (D) and mutant allele (d) were diagnosed in 464 fattened progeny of three carrier sires (Dd), and 140 cows of Sire 1 whose common ancestor was revealed as Dd. The progeny were composed of 230 normal (DD) and 234 carrier (Dd). The cows were composed of 60 DD and 80 Dd. The association analysis was carried out among the CL-16 genotypes, and carcass traits and predicted breeding value for carcass traits using the 464 progeny and 140 cows, respectively. Analysis of variance was performed considering the CL-16 genotypes, sire, sex, farm and age (linear and quadratic) as the source of variance in order to evaluate factors affecting carcass traits. Significant differences in BMS number, carcass weight, area of rib eye, and rib thickness were observed between the sires. Significant differences in carcass weight were observed in the influence of sex. Significant differences in BMS number, carcass weight, and rib thickness were observed between farms. The regression of any carcass traits on final age (linear and quadratic) was not statistically significant. The significant association of the CL-16 genotypes were not seen in the carcass traits after the effects of environmental factors were eliminated. Moreover, a significant association of the genotypes was not seen (P>0.05) with predicted breeding value for carcass traits in cows of Sire-1. Furthermore, these carcass traits had no significant linkage with the approximately 30 cM region including CL-16 locus in a half-sib family of Sire-1 comprising 136 offspring (P>0.05). Therefore, exclusion of the mutant allele d from Japanese Black cattle population would not deteriorate the carcass traits.

DOI： 10.2508/chikusan.73.19

DOI： 10.1292/jvms.68.319_references_DOI_3Tfx6jEYktXsr2H9WH83lLPf6qK

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I6266493

多サンプル間での遺伝子発現量の比較に適用可能な半定量的デイファレンシャルディスプレイ法の確立

永田幸洋, 揖斐隆之, 谷口幸雄, 山田宜永, 佐々木義之

日本畜産学会大会講演要旨 99th 46 2001.8

Gibbs Samplingによる競走馬における上部気道疾患の遺伝率の推定

揖斐隆之, 三宅武, 楠瀬良, 帆保誠二, 沖博憲, 佐々木義之

日本畜産学会大会講演要旨 99th 45 2001.8

近交係数の上昇を抑え遺伝的改良を最大にする育種計画

揖斐隆之, 佐々木義之

日本畜産学会大会講演要旨 98th 93 2001.3

ラット最長筋内脂肪量の組織化学的測定法の確立ならびにOLETF,F344およびそれらのF1間の比較

田之村秀樹, 三宅武, 谷口幸雄, 揖斐隆之, 真鍋昇, 山田宜永, 佐々木義之

日本畜産学会大会講演要旨 97th 61 2000.3

近交係数の上昇を抑え遺伝的改良を最大にする育種システム

揖斐隆之, 守田智, 佐々木義之

日本畜産学会大会講演要旨 96th 67 1999.9

Transition of group with construction of genetic evaluation system in Kumamoto based brown-haired Japanese species.

揖斐隆之, 守田智, 守屋和幸, 佐々木義之

肉用牛研究会報 66 ( 66 ) 8 - 9 1998.12

肉牛育種システムの構築 2. 近交係数の上昇を抑え遺伝的改良を最大にする交配システム

揖斐隆之, 守田智, 守屋和幸, 佐々木義之

システム農学会シンポジウム要旨集 1998 44 - 45 1998.11

肉牛育種システムの構築 1. 遺伝的評価システムの構築に伴なう集団の変遷

佐々木義之, 揖斐隆之, 守田智, 守屋和幸

システム農学会シンポジウム要旨集 1998 42 - 43 1998.11

Elucidation of population structure of brown-haired Japanese variant group in Kumamoto prefecture based on the contribution ratio on the genetics of the typical founder bull.

揖斐隆之, 守屋和幸, 松本道夫, 木場俊太郎, 佐々木義之

日本畜産学会大会講演要旨 91st 175 1996.3

熊本県褐毛和種集団の集団構造の変化ならびに産地間の遺伝的関連性

佐々木義之, 揖斐隆之, 守屋和幸, 高柳誠二, 松本道夫, 木場俊太郎

日本畜産学会大会講演要旨 90th 144 1995.3

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