Faculty Profiles - IBI Takayuki

MISC - IBI Takayuki

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岡山県固有の黒毛和種希少系統の遺伝学的特徴の解析

米田一裕, 奥田ゆう, Siqintuya, 西牧孝洋, 松本大和, 宮崎義之, 揖斐隆之, 辻岳人, 国枝哲夫

日本畜産学会報 87 ( 1 ) 1‐10(J‐STAGE) - 10 2016.2

Language：Japanese Publisher：公益社団法人日本畜産学会

The effective population size of Japanese Black cattle has been significantly reduced and maintaining the genetic diversity of the population is important for breeding of Japanese Black cattle. For the conservation of genetic diversity, use of different lines of the breed with unique genetic characteristics for breeding, instead of intensive use of sires of few particular lines as common in current breeding of Japanese Black cattle, will be effective to prevent the genetic homogenization of the population. In the present study, we performed genetic characterization a population of a rare line of Japanese Black cattle, which has been originated from ancestral "Tsuru-ushi" in Okayama prefecture. By using microsatellite markers, allelic richness, and average observed and expected heterozygosity are 3.48, 0.514, and 0.511, respectively, and these values were lower than those of most of the Japanese Black cattle local subpopulations compared. The result of the clustering analyses indicated that the animals of the rare line formed a single group with a cluster that was clearly distinguished from the other populations. Sequence analysis of mitochondrial D-loop region revealed that only two haplotypes were observed in the population and 80% of the animals in the population possess a single haplotype. However, the other haplotype was a novel unique haplotype that has not been reported in cattle. Genotyping of six genes associated with important traits revealed that SREBP1 and NCAPG loci were fixed for a single allele in the population and more than 90% of animals possess an allele of MC1R locus. These findings indicated that while genetic diversity of the population of the rare line is lower than those of the Japanese Black cattle local subpopulations, this population has unique genetic characteristics that were distinguished from the other populations and, therefore, the rare line is important for maintaining genetic diversity of Japanese Black cattle.

DOI： 10.2508/chikusan.87.1

Survey on the undernourished university students who tend to lack breakfast; a proposal for a novel viewpoint for the improvement

田村隆, 揖斐隆之, 稲垣賢二, 久保康隆, 奥田潔

岡山大学農学部学術報告(Web) 105 ( 105 ) 1-5 (WEB ONLY) - 5 2016.2

Language：Japanese Publisher：岡山大学農学部

This study investigated the current status and causes underneath the life of university students who tend to lack breakfast at a relatively high frequency, and statistical analysis on consequences leading to such lack of well-nourished eating habitat in their university life. In October 2014, self-assessed questionnaires were administered to over 150 faculty students. It contained questions about breakfast habits, time allowance for the morning class, and lunchtime setting in their high school timetable. Breakfast states were clearly separated in three groups : 68% of students regularly have breakfast throughout the weekdays, 21% students skipping the breakfast occasionally, and 11% student no habit for breakfast at all. The survey on the high school lives revealed that 70% students used to have lunch 30 min later than the lunchtime set in the university timetable, 7% of them had the lunch time even more than 1 h later. Lunchtime varies among high schools, and statistical significance was revealed (p<0.01) that schools with higher deviation scores tend have late lunch beyond 12: 30. Accordingly, university students were given directions to prepare for the timetable reform on postulation of having lunch time over one o'clock. After continuous survey on the breakfast habits during the second semester, more than 90% of students established the habit of breakfast regularly in their university lives with the improved consciousness toward well-balanced healthy breakfast contents for their higher level of education quality.

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「豊味(うま)いの証」豊後牛肉生産技術の確立―牛肉中のオレイン酸含有率を取り入れた産肉能力育種価手法の検討―

志村英明, 利光昭彦, 衞藤央好, 安高康幸, 揖斐隆之

九州農業研究発表会専門部会発表要旨集 78th 71 2015.8

岡山県のジャージー集団におけるBCO2遺伝子の新たな変異と脂肪淡黄色との関連性

清水佑起, 石倉健一, 揖斐隆之, 国枝哲夫, 辻岳人

日本畜産学会大会講演要旨 119th 115 2015.3

オレイン酸含有率を用いた新たな育種価評価の確立

利光昭彦, 手島久智, 衛藤央好, 三輪友樹, 藤田達男, 揖斐隆之

九州沖縄農業試験研究の成果情報(Web) 2015 WEB ONLY 2015

Allelic distributions of genes involved in economical traits, hereditary disorder, and coat color in a population of Kuchinoshima cattle

SIQINTUYA, 西牧孝洋, 揖斐隆之, 辻岳人, 米田一裕, 大島一郎, 片平清美, 万年英之, 下桐猛, 印牧美佐生, 国枝哲夫

動物遺伝育種研究 42 ( 1 ) 11 - 19 2014.7

Language：Japanese Publisher：日本動物遺伝育種学会

Kuchinoshima cattle is a breed of the unique feral cattle originated from grazing Japanese native cattle in Kuchinoshima island of Tokara Islands in Kagoshima prefecture during Meiji and Taisho periods. The distribution and frequencies of alleles of the genes associated with economical traits and hereditary disorders have been reported in populations of Japanese Black cattle that is a major beef cattle breed in Japan and Mishima cattle that is another Japanese native cattle breed in Mishima island, but those of Kuchinoshima cattle have not been reported. Therefore, we investigated the allelic distribution and frequencies of the genes associated with economical traits, hereditary disorders, and coat color to reveal genetic characteristic of Kuchinoshima cattle by comparison of the distributions among Kuchinoshima, Japanese Black, and Mishima cattle. By using 32 DNA samples collected from Kuchinoshima cattle, we genotyped NCAPG, FASN, SCD, and SREBP-1 genes associated with economical traits including carcass weight and fatty acid composition, F11 gene responsible for factor XI deficiency, and MC1R gene involved in coat color determination by PCR or PCR-RFLP assay and compared these results with those of Japanese Black and Mishima cattle. As a result, we found that while both G and T alleles of NCAPG locus were observed in the population of Japanese black cattle, the locus was fixed to T allele in the populations of Kuchinoshima and Mishima cattle, and that while both TW and AR alleles of FASN locus were observed in Japanese black cattle, the locus was fixed to AR allele in Kuchinoshima cattle and TW allele in Mishima cattle. For the SCD and SREBP-1 loci, both A and V alleles of SCD locus were observed in Japanese Black, Kuchinoshima, and Mishima cattle, and both S and L alleles SREBP-1 locus were observed in Japanese Black and Mishima cattle but fixed to L allele in Kuchinoshima cattle. Furthermore, while both (+) and (-) alleles of F11 locus were observed in the population of Japanese black cattle, the locus was fixed to (+) allele in the populations of Kuchinoshima and Mishima cattle. Finally, we observed E + and e alleles of MC1R locus in Kuchinoshima cattle, while E D and E + allele but no e allele in Japanese Black cattle and only E + allele in Mishima cattle were observed. These findings indicate that Kuchinoshima cattle comprises a population of Japanese native cattle possessing unique genetic characteristics different from those of Japanese Black and Mishima cattle, which has been formed under geographically isolated circumstance with relatively small population without influence of foreign breeds.

DOI： 10.5924/abgri.42.11

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和牛の胚死滅を引き起こす劣性変異の探索

佐々木慎二, 渡邊敏夫, 揖斐隆之, 杉本喜憲

日本畜産学会大会講演要旨 116th 155 2013.3

黒毛和種の胚死滅を引き起こす劣性変異の探索

佐々木慎二, 渡邊敏夫, 揖斐隆之, 杉本喜憲

動物遺伝育種研究 40 ( 2 ) 69 2012.12

黒毛和種における第XI因子欠乏症と異常産,新生仔死との関連

斯琴図雅, 緒方良彦, 森友靖生, 三浦潔, 西牧孝洋, 揖斐隆之, 国枝哲夫

動物遺伝育種研究 40 ( 2 ) 78 2012.12

黒毛和種の枝肉形質に対するSCD,SREBP1,FASNおよびNCAPG遺伝子多型の関連解析

西牧孝洋, 揖斐隆之, 斯琴図雅, 小林直彦, 松橋珠子, 今井佳積, 松井茉由, 上村圭一, 衛藤央好, 渡邉直人, 藤田達男, 齊藤陽介, 小松智彦, 万年英之, 笹崎晋史, 国枝哲夫

動物遺伝育種研究 40 ( 2 ) 71 2012.12

黒毛和種・雌牛の受胎率を低下させる遺伝的要因の解明

佐々木慎二, 揖斐隆之, 松橋珠子, 池田省吾, 小林直彦, 杉本喜憲

日本畜産学会大会講演要旨 115th 166 2012.3

黒毛和種における各地域集団の遺伝的多様性と類縁関係の解析

西牧孝洋, 揖斐隆之, 田邉義弘, 宮崎義之, 小林直彦, 松橋珠子, 秋山敬孝, 吉田恵実, 今井佳積, 松井茉由, 上村圭一, 渡邉直人, 藤田達男, 齊藤陽介, 万年英之, 笹崎晋史, 国枝哲夫

動物遺伝育種研究 39 ( 2 ) 142 2011.12

黒毛和種における雌牛の繁殖能力に関するゲノム解析

佐々木慎二, 揖斐隆之, 松橋珠子, 池田省吾, 小林直彦, 渡邊敏夫, 杉本喜憲

動物遺伝育種研究 39 ( 2 ) 132 2011.12

Genome-Wide Association Study for Female Reproductive Efficiency in Japanese Black Cattle

Shinji Sasaki, Takayuki Ibi, Toshio Watanabe, Yoshikazu Sugimoto

BIOLOGY OF REPRODUCTION 85 2011.7

Estimation of genetic parameters for beef marbling score and marbling on top round of Japanese Black

揖斐隆之

岡山大学農学部学術報告 100 61 - 65 2011.2

Language：Japanese Publisher：岡山大学農学部

Japanese Black cattle are the predominant beef breed in Japan. This breed has excellent meat quality, especially degree of (intramuscular) marbling. Degree of marbling was measured at the 6th to 7th rib section and according to the Beef Marbling Standard with scores 1 to 12 (so-called BMS), with number 12 being the best. BMS is used with business deal of beef carcass and has strongly effect on selling price of carcass. On the other hands, more degree of marbling on top round (MTR) is better carcass unit price in same BMS, because of good price of part of thigh. MTR has been focused in recent years. However, MTR is unclear how to evaluate, therefore, collection of records and genetic evaluations are not enough. In this study, records of MTR were collected and then, genetic parameters for BMS and MTR of Japanese Black were estimated. The original data were 11,052 carcass records collected from April in 2008 to June in 2009 at the Agura Farm. Only information from abattoirs having more than 20 records and feedlot farms having more than 10 records were used. This resulted in a total of 10,990 records. In this study, pedigree information was traced back to two generations. An animal model that included all relationships among all animals was used as a statistical model. The fixed effects included sex, farm, abattoir and the combination of slaughter year and month. Slaughter age was fitted as linear covariables. Genetic parameters were estimated using REMLF90 and THRGIBBS1F90 programs. The estimates of heritability were moderately high (0.37 to 0.44) for MTR treated as threshold traits. The estimates of phenotypic and genetic correlations between BMS and MTR were very high (0.87 and 0.98, respectively). These indicate that MTR can be improved genetically and improvement of MTR can treat BMS as indicator.

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黒毛和午におけるBMSおよびモモヌケの遺伝的パラメータの推定

揖斐隆之

岡山大學農學部學術報告 = Scientific report of the Faculty of Agriculture, Okayama University ( 100 ) 61 - 65 2011.2

黒毛和種における雌牛の繁殖能力に関するゲノム解析

佐々木慎二, 揖斐隆之, 渡邊敏夫, 松橋珠子, 中藤由紀, 小林直彦, 杉本喜憲

動物遺伝育種研究 38 ( 2 ) 131 2010.12

3.黒毛和牛におけるBMSおよびモモヌケの遺伝的パラメータの推定

揖斐隆之

肉用牛研究会報 88 ( 88 ) 67 - 68 2010.1

黒毛和牛におけるBMSおよびモモヌケの遺伝的パラメータの推定

揖斐隆之

肉用牛研究会報 88 67 - 68 2010.1

性判別技術や双子生産技術の導入が黒毛和種繁殖生産の経済性に及ぼす影響

大石風人, 揖斐隆之, 熊谷元, 広岡博之

日本畜産学会大会講演要旨 110th 158 2009.3

安愚楽共済牧場における家畜育種の取り組み

揖斐隆之

動物遺伝育種研究 36 ( 1 ) 29 - 37 2008.6

繁殖雌牛の経産回数と繁殖性,割引率の変化が和牛繁殖生産の経済性に及ぼす影響

大石風人, 揖斐隆之, 熊谷元, 広岡博之

日本畜産学会大会講演要旨 109th 95 2008.3

黒毛和種の子牛における初期の生存性に関する遺伝的パラメータの推定

揖斐隆之, 広岡博之

日本畜産学会大会講演要旨 109th 95 2008.3

Breeding programs of Japanese Black cattle in Agura Farm

IBI Takayuki

The journal of animal genetics 36 ( 1 ) 29 - 37 2008

黒毛和種における広域種牛評価の精度に関する研究

中岡博史, 藤中邦則, 渡邉直人, 川田啓介, 揖斐隆之, 佐々江洋太郎, 佐々木義之

動物遺伝育種研究 35 ( 2 ) 232 2007.12

和牛繁殖生産における繁殖雌牛の供用年数と繁殖率の変化が生産効率に及ぼす影響

大石風人, 揖斐隆之, 熊谷元, 広岡博之

日本畜産学会大会講演要旨 108th 38 2007.9

肉用牛経営の個別属性や経営意識が家畜排泄物の処理・利用に及ぼす影響

長命洋佑, 揖斐隆之, 仙田徹志, 森佳子, 広岡博之

農林業問題研究 43 ( 1 ) 51 - 56 2007.6

家畜育種における遺伝と環境の交互作用

揖斐隆之, 広岡博之

動物遺伝育種研究 35 ( 1 ) 25 - 31 2007.6

黒毛和種の妊娠期間に関する遺伝的パラメータの推定と生時体重や枝肉形質との遺伝的関連性

揖斐隆之, 広岡博之

日本畜産学会大会講演要旨 107th 64 2007.3

肉用牛経営における潜在的窒素負荷量が家畜排泄物の処理・利用の意向に及ぼす影響

長命洋佑, 揖斐隆之, 熊谷元, 広岡博之

日本畜産学会大会講演要旨 107th 156 2007.3

肉用牛経営の個別属性や経営意識が家畜排泄物の処理・利用に及ぼす影響

長命洋佑, 揖斐隆之, 仙田徹志, 森佳子, 広岡博之

農林業問題研究 43 ( 1 ) 51 - 56 2007

Genotype × environment interaction for animal breeding

IBI Takayuki, HIROOKA Hiroyuki

The journal of animal genetics 35 ( 1 ) 25 - 31 2007

和牛繁殖生産における繁殖雌牛の供用年数と生産効率との関係

大石風人, 熊谷元, 揖斐隆之, 広岡博之

システム農学会シンポジウム要旨集 2007 22 - 23 2007

黒毛和種集団の広域種牛評価によるサブクラス間不均一分散の補正の有効性

中岡博史, 三宅武, 揖斐隆之, 佐々江洋太郎, 山田宜永, 佐々木義之

動物遺伝育種研究 34 ( 2 ) 119 2006.12

Effects of Attributes and Consciousness of Fattening Farms Households on Carcass Prices and Traits

CHOUMEI Yousuke, IBI Takayuki, HIROOKA Hiroyuki

Nihon Chikusan Gakkaiho 77 ( 2 ) 289 - 294 2006.5

Language：Japanese Publisher：Japanese Society of Animal Science

The objective of this study was to investigate effects of attributes and consciousness of farm households on prices and traits of carcass sold from the farms. Questionnaire surveys were conducted to farms under the Agura Co-operative Farm and 91 questionnaires were available for the present analysis. Best linear unbiased estimations (BLUEs) of each farm for carcass prices and traits were estimated using 20,346 carcass records collected from January 2004 to March 2005 at the Agura co-operation Farm. The BLUEs were analyzed by least squares procedures using a model containing attributes and consciousness of farm households obtained from questionnaires as sources of variation. The result showed that farm households who make great account of both income and joy of life in keeping animals can produce carcasses with higher quality. In addition, it was shown that the effect of farm size on carcass unit price was quadratic ; as farm size increased, carcass unit price decreased and increased beyond the size of more than 932 heads.

DOI： 10.2508/chikusan.77.289

黒毛和種牛の産肉性に関する選抜指数の設定

DORONBEKOV Kanat, 揖斐隆之, 佐々江洋太郎, 三宅武, 佐々木義之

日本畜産学会大会講演要旨 106th 58 2006.3

Gibbs Samplingによる黒毛和種枝肉におけるかしの遺伝率推定

揖斐隆之, 三宅武, 佐々木義之

肉用牛研究会報 ( 80 ) 69 - 73 2005.12

Estimation of heritability for defects of beef carcass using Gibbs sampling in Japanese Black cattle

IBI Takayuki, MIYAKE Takeshi, SASAKI Yoshiyuki

Bulletin of Beef Cattle Science 80 69 - 73 2005.12

枝肉形質間の遺伝相関係数に基づく肉牛品種間系統樹の作成ならびに遺伝的距離の検討

三宅武, 中岡博史, 小谷基, 広岡博之, 小邦朋子, 住尾善彦, 松本道夫, 伊藤雅之, 倉原貴美, 揖斐隆之, 佐々江洋太郎, 藤中邦則, 太田垣進, 道後泰治, 佐々木義之

肉用牛研究会報 80 ( 80 ) 23 - 25 2005.12

黒毛和種集団の全国的な種牛評価におけるサブクラス間の不均一分散の影響~不均一分散が複数形質を考慮した総合評価に及ぼす影響

中岡博史, 揖斐隆之, 佐々江洋太郎, 佐々木義之

肉用牛研究会報 80 ( 80 ) 4 - 6 2005.12

黒毛和牛における枝肉価格に関する遺伝的パラメータのBSEの発生による変遷

揖斐隆之, 広岡博之

日本畜産学会大会講演要旨 105th 57 2005.8

全国的な黒毛和種集団の種牛評価における不均一分散の補正

中岡博史, 揖斐隆之, 佐々江洋太郎, 佐々木義之

日本畜産学会大会講演要旨 105th 52 2005.8

サブクラス間の分散成分不均一性を考慮した種牛評価法とその予測育種価への影響

中岡博史, 揖斐隆之, 佐々江洋太郎, 佐々木義之

日本畜産学会大会講演要旨 104th 43 2005.3

Gibbs Samplingによる黒毛和牛枝肉におけるかしの遺伝率推定

揖斐隆之, 三宅武, 佐々江洋太郎, 佐々木義之

日本畜産学会大会講演要旨 104th 84 2005.3

枝肉形質間の遺伝相関係数に基づく肉牛品種間系統樹の作成ならびに遺伝的距離の検討

肉用牛研究会大会 43rd 23 - 26 2005

黒毛和種集団の全国的な種牛評価におけるサブクラス間の不均一分散の影響~不均一分散が複数形質を考慮した総合評価に及ぼす影響

中岡博史, 揖斐隆之, 佐々江洋太郎, 佐々木義之

肉用牛研究会大会 43rd 1 - 4 2005

黒毛和種における全国的な種牛評価の可能性および数学モデルに関する研究

小谷基, 中岡博史, 成田暁, 揖斐隆之, 佐々江洋太郎, 佐々木義之

日本畜産学会報 75 ( 3 ) 353 - 361 2004.8

Language：Japanese Publisher：公益社団法人日本畜産学会

Comparisons of breeding stock among the Japanese Black cattle across regions were impeded because breeding programs and genetic evaluations were carried out primarily within regions. A nationwide connectedness was missing. The objective of this study was to investigate the possibility of a national genetic evaluation of breeding stocks based on appropriate mathematical models and suited material. Goodness of fit of the models was measured by the prediction error variance, the Akaike&rsquo;s information criterion and coefficient of determination. The data consisted of field records on carcass traits (beef marbling standard number, BMS No. ; carcass weight, CW ; rib-eye area, REA ; rib thickness, RT ; subcutaneous fat thickness, SFT) of fattened Japanese Black cattle collected from 1997 to 2002 among the nationwide fattening farms of Agura Kyosai Bokujo, Inc. (N=49,810). The pedigree of those animals was surveyed, too. More than 95% of all possible pairs of fattening farms have beef animals with common sires. Further beef animals from different prefectures were dispersed over fattening farms so that comparisons among all prefectures were possible. Six mathematical models were applied, which differed in the way how each of the fixed effects was taken into account. The estimated heritabilities do not vary significantly among the applied mathematical models (h2 for BMS No. : 0.43 ; CW : 0.45-0.49 ; REA : 0.32-0.33 ; RT : 0.31-0.33 ; SFT : 0.46-0.47). The models with the best goodness of fit are obtained when all the fixed effects are taken into account as a combination effect or when all the fixed effects but the effect of sex are taken into account as a combination of them. We were able to demonstrate that the genetic connectedness among subpopulations is sufficient in the involved fattening farms in order to establish a genetic evaluation on a national scale. A nationwide evaluation of the Japanese Black breeding stocks will enhance the genetic improvement of the breed by a more efficient selection of excellent sires and dams.

DOI： 10.2508/chikusan.75.353

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黒毛和種および褐毛和種の枝肉形質に関する遺伝性の類似

三宅武, 揖斐隆之, 松本道夫, 藤中邦則, 山田宜永, 佐々木義之

日本畜産学会大会講演要旨 103rd 137 2004.3

黒毛和種の枝肉形質における遺伝と管理システムの交互作用に関する研究

揖斐隆之, 広岡博之, 佐々江洋太郎, 佐々木義之

日本畜産学会大会講演要旨 103rd 138 2004.3

Gibbs Samplingによる競走馬における上喉頭片麻痺の遺伝率推定

揖斐隆之, 三宅武, 帆保誠二

馬の科学 41 ( 3 ) 155 - 162 2004

黒毛和種の枝肉形質における遺伝と地域の交互作用に関する研究

揖斐隆之, 広岡博之, 佐々江洋太郎, 佐々木義之

日本畜産学会大会講演要旨 102nd 39 2003.9

黒毛和種における全国的な種牛評価に関する研究その1 遺伝的結合度の検討と数学モデルの遺伝率推定値への影響

中岡博史, 小谷基, 成田暁, 揖斐隆之, 佐々江洋太郎, 佐々木義之

日本畜産学会大会講演要旨 102nd 39 2003.9

黒毛和種における全国的な種牛評価に関する研究その2 最適な数学モデルに関する検討

小谷基, 中岡博史, 成田暁, 揖斐隆之, 佐々江洋太郎, 佐々木義之

日本畜産学会大会講演要旨 102nd 39 2003.9

Estimation of heritability of laryngeal Hemiplegia in the Thoroughbred horse by gibbs sampling

Takayuki Ibi, Takeshi Miyake, Seiji Hobo, Hironori Oki, Nobushige Ishida, Yoshiyuki Sasaki

Journal of Equine Science 14 ( 3 ) 81 - 86 2003.9

Language：English

Laryngeal Hemiplegia (LH) leads to a reduction in performance and because of it many promising racehorses have been forced to end their racing career. Therefore it is important for breeding good racehorses to estimate the heritability of LH. In this study, the computer program was developed based on the Bayesian analysis with Gibbs sampling for estimating the heritability of categorical traits assuming liability. A total of 706 records with LH-grade in Thoroughbreds aged 2 to 5 years were assigned for the genetic analysis. LH-grades consisted of five severity classes from 0 to 4. Racehorse breeders are often interested in whether the genetic effect controlling a complex disorder is present in the population. To answer this question, the binary trait analysis would be also useful. The heritability of LH-grade in the Thoroughbred horse was then also estimated as a binary or as a categorical trait. The mode values of the posterior distributions of heritability were 0.23 and 0.20 for the binary and the categorical trait, respectively. The fact that in the Thoroughbred population studied LH is at least partially controlled by genetic factors leads to the suggestion that when applying adequate breeding measures the prevalence of LH will be able to be reduced.

DOI： 10.1294/jes.14.81

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Estimation of heritability of laryngeal Hemiplegia in the Thoroughbred horse by gibbs sampling

Takayuki Ibi, Takeshi Miyake, Seiji Hobo, Hironori Oki, Nobushige Ishida, Yoshiyuki Sasaki

Journal of Equine Science 14 ( 3 ) 81 - 86 2003.9

Language：English Publisher：日本ウマ科学会

DOI： 10.1294/jes.14.81

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Genetic Statistical Analysis on Carcass Traits and Daily Gain of Japanese Black Cattle in Southern Part of Iwate Prefecture

川田啓介, 兼松重任, 黒沢弥悦, 揖斐隆之, 佐々木義之

日本畜産学会報 74 ( 2 ) 187 - 193 2003.5

Language：Japanese Publisher：公益社団法人日本畜産学会

In order to elucidate the properties for the breeding of Japanese Black Cattle population in the southern part of Iwate prefecture, genetic parameters, breeding values and genetic trends of carcass traits and daily gain (DG) were estimated using 3,467 field records from the steers and heifers fattened in Maesawa, Iwate prefecture, from 1994 to 1999. Only farmers who shipped more than 30 animals during this period were chosen. It was remarkably noticed that best linear unbiased estimations for beef marbling standard number (BMS) have decreased successively for years. The heritability estimates for carcass traits and DG were as follows : 0.66 for BMS, 0.51 for DG, 0.64 for carcass weight, 0.36 for rib-eye area, 0.25 for rib thickness and 0.33 for subcutaneous fat thickness. The genetic correlation, between BMS and other traits were as follows : 0.1 for DG, 0.18 for the carcass weight, 0.48 for the rib-eye area, 0.37 for the rib thickness and -0.24 for the subcutaneous fat thickness. As far as the genetic trends were concerned, improvements on the BMS and the subcutaneous fat thickness were highly regarded, while the carcass weight was rarely taken into consideration since 1991. On the other hand, the phenotypic average for BMS of animals fattened in Maesawa has tended to decrease since 1991. Based on the present results, factors other than genetic improvement could be considered the cause of the decrease.

DOI： 10.2508/chikusan.74.187

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黒毛和種における全国的な種牛評価のための数学モデルに関する予備的検討

小谷基, 成田暁, 揖斐隆之, 佐々江洋太郎, 佐々木義之

日本畜産学会大会講演要旨 101st 107 2003.3

遺伝子発現量の多サンプル間での比較のためのディファレンシャルディスプレイ法に関する検討

永田幸洋, 揖斐隆之, 谷口幸雄, 山田宜永, 佐々木義之

動物遺伝育種研究 30 ( 1 ) 11 - 18 2002.11

Gibbs samplingにより混合遺伝子モデルを用いたQTL遺伝子型推定法の検討

大野涼太, 成田暁, 揖斐隆之, 佐々木義之

日本畜産学会大会講演要旨 100th 108 2002.3

非近交系集団における混合遺伝モデルを用いた複数のQTL解析法に関する研究 RJMCMCを応用したQTL数の推定

成田暁, 大野涼太, 揖斐隆之, 佐々木義之

日本畜産学会大会講演要旨 100th 109 2002.3

Association Analysis between the Deletion Mutant Allele of Claudin‐16 Deficiency and Carcass Traits in Japanese Black Cattle.

小林直彦, 平野貴, 揖斐隆之, 大谷健, 杉本喜憲

Animal Science Journal 73 ( 1 ) 19 - 23 2002.2

Language：Japanese Publisher：公益社団法人日本畜産学会

Claudin-16 (CL-16) deficiency is an autosomal recessive disease in Japanese black cattle. The association analysis between the deletion mutant allele (d) of CL-16 deficiency and carcass traits was investigated. Normal allele (D) and mutant allele (d) were diagnosed in 464 fattened progeny of three carrier sires (Dd), and 140 cows of Sire 1 whose common ancestor was revealed as Dd. The progeny were composed of 230 normal (DD) and 234 carrier (Dd). The cows were composed of 60 DD and 80 Dd. The association analysis was carried out among the CL-16 genotypes, and carcass traits and predicted breeding value for carcass traits using the 464 progeny and 140 cows, respectively. Analysis of variance was performed considering the CL-16 genotypes, sire, sex, farm and age (linear and quadratic) as the source of variance in order to evaluate factors affecting carcass traits. Significant differences in BMS number, carcass weight, area of rib eye, and rib thickness were observed between the sires. Significant differences in carcass weight were observed in the influence of sex. Significant differences in BMS number, carcass weight, and rib thickness were observed between farms. The regression of any carcass traits on final age (linear and quadratic) was not statistically significant. The significant association of the CL-16 genotypes were not seen in the carcass traits after the effects of environmental factors were eliminated. Moreover, a significant association of the genotypes was not seen (P>0.05) with predicted breeding value for carcass traits in cows of Sire-1. Furthermore, these carcass traits had no significant linkage with the approximately 30 cM region including CL-16 locus in a half-sib family of Sire-1 comprising 136 offspring (P>0.05). Therefore, exclusion of the mutant allele d from Japanese Black cattle population would not deteriorate the carcass traits.

DOI： 10.2508/chikusan.73.19

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Differential display method used for the comparison of mRNA expression level among many samples

NAGATA Yukihiro, IBI Takayuki, TANIGUCHI Yukio, YAMADA Takahisa, SASAKI Yoshiyuki

The journal of animal genetics 30 ( 1 ) 11 - 18 2002

Language：Japanese Publisher：Japanese Society of Animal Breeding and Genetics

A reproducibility of experimental data is important for application of differential display method to the comparison of mRNA expression level among many samples. First, we developed the optimum method to evaluate size (BP) and yield (IOD) of PCR products by analyzing an electrophoretic image. BP (A), which is a way to calculate size of each product by using 3 molecular markers loaded in extreme and central lanes of gel as references, was optimal for size evaluation. Further, IOD (A) and IOD (P), respectively, which are way to use a measured absolute value of signal intensity of each product, and to use a relative value of signal intensity of each product as proportion of total sum of all products in an identical lane, as yield, were determined as the suboptimum and optimum evaluation methods. Next, we performed an analysis of variance of intergel, interlane, and interreaction effects, when using the optimum method. We detected statistically significant effects for intergel and interlane, but not for interreaction, when using BP (A) method. As for IOD (A) and IOD (P), significant effects for intergel and interreaction were observed, but not for interlane. Finally, we evaluated the replicate pattern that is suitable for a largescale gene expression profiling, by examining coefficient of variation of combined effects for BP and IOD obtained by 4 different replicate patterns.

DOI： 10.5924/abgri2000.30.11

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Association Analysis between the Deletion Mutant Allele of Claudin-16 Deficiency and Carcass Traits in Japanese Black Cattle

KOBAYASHI Naohiko, HIRANO Takashi, IBI Takayuki, OHTANI Tuyoshi, SUGIMOTO Yoshikazu

Nihon Chikusan Gakkaiho 73 ( 1 ) 19 - 23 2002

Language：Japanese Publisher：Japanese Society of Animal Science

Claudin-16 (CL-16) deficiency is an autosomal recessive disease in Japanese black cattle. The association analysis between the deletion mutant allele (d) of CL-16 deficiency and carcass traits was investigated. Normal allele (D) and mutant allele (d) were diagnosed in 464 fattened progeny of three carrier sires (Dd), and 140 cows of Sire 1 whose common ancestor was revealed as Dd. The progeny were composed of 230 normal (DD) and 234 carrier (Dd). The cows were composed of 60 DD and 80 Dd. The association analysis was carried out among the CL-16 genotypes, and carcass traits and predicted breeding value for carcass traits using the 464 progeny and 140 cows, respectively. Analysis of variance was performed considering the CL-16 genotypes, sire, sex, farm and age (linear and quadratic) as the source of variance in order to evaluate factors affecting carcass traits. Significant differences in BMS number, carcass weight, area of rib eye, and rib thickness were observed between the sires. Significant differences in carcass weight were observed in the influence of sex. Significant differences in BMS number, carcass weight, and rib thickness were observed between farms. The regression of any carcass traits on final age (linear and quadratic) was not statistically significant. The significant association of the CL-16 genotypes were not seen in the carcass traits after the effects of environmental factors were eliminated. Moreover, a significant association of the genotypes was not seen (P>0.05) with predicted breeding value for carcass traits in cows of Sire-1. Furthermore, these carcass traits had no significant linkage with the approximately 30 cM region including CL-16 locus in a half-sib family of Sire-1 comprising 136 offspring (P>0.05). Therefore, exclusion of the mutant allele d from Japanese Black cattle population would not deteriorate the carcass traits.

DOI： 10.2508/chikusan.73.19

DOI： 10.1292/jvms.68.319_references_DOI_3Tfx6jEYktXsr2H9WH83lLPf6qK

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I6266493

多サンプル間での遺伝子発現量の比較に適用可能な半定量的デイファレンシャルディスプレイ法の確立

永田幸洋, 揖斐隆之, 谷口幸雄, 山田宜永, 佐々木義之

日本畜産学会大会講演要旨 99th 46 2001.8

Gibbs Samplingによる競走馬における上部気道疾患の遺伝率の推定

揖斐隆之, 三宅武, 楠瀬良, 帆保誠二, 沖博憲, 佐々木義之

日本畜産学会大会講演要旨 99th 45 2001.8

近交係数の上昇を抑え遺伝的改良を最大にする育種計画

揖斐隆之, 佐々木義之

日本畜産学会大会講演要旨 98th 93 2001.3

ラット最長筋内脂肪量の組織化学的測定法の確立ならびにOLETF,F344およびそれらのF1間の比較

田之村秀樹, 三宅武, 谷口幸雄, 揖斐隆之, 真鍋昇, 山田宜永, 佐々木義之

日本畜産学会大会講演要旨 97th 61 2000.3

近交係数の上昇を抑え遺伝的改良を最大にする育種システム

揖斐隆之, 守田智, 佐々木義之

日本畜産学会大会講演要旨 96th 67 1999.9

Transition of group with construction of genetic evaluation system in Kumamoto based brown-haired Japanese species.

揖斐隆之, 守田智, 守屋和幸, 佐々木義之

肉用牛研究会報 66 ( 66 ) 8 - 9 1998.12

肉牛育種システムの構築 2. 近交係数の上昇を抑え遺伝的改良を最大にする交配システム

揖斐隆之, 守田智, 守屋和幸, 佐々木義之

システム農学会シンポジウム要旨集 1998 44 - 45 1998.11

肉牛育種システムの構築 1. 遺伝的評価システムの構築に伴なう集団の変遷

佐々木義之, 揖斐隆之, 守田智, 守屋和幸

システム農学会シンポジウム要旨集 1998 42 - 43 1998.11

Elucidation of population structure of brown-haired Japanese variant group in Kumamoto prefecture based on the contribution ratio on the genetics of the typical founder bull.

揖斐隆之, 守屋和幸, 松本道夫, 木場俊太郎, 佐々木義之

日本畜産学会大会講演要旨 91st 175 1996.3

熊本県褐毛和種集団の集団構造の変化ならびに産地間の遺伝的関連性

佐々木義之, 揖斐隆之, 守屋和幸, 高柳誠二, 松本道夫, 木場俊太郎

日本畜産学会大会講演要旨 90th 144 1995.3

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