Updated on 2025/08/20

写真a

 
IBI Takayuki
 
Organization
Faculty of Environmental, Life, Natural Science and Technology Associate Professor
Position
Associate Professor
External link

Degree

  • Agriculture ( 2002.3   Kyoto University )

  • 博士（農学） ( 2002.3   京都大学 )

Research Interests

  • Quantitative genetics

  • Population genetics

  • Animal breeding

Research Areas

  • Life Science / Animal production science  / animal breeding and genetics

Education

  • Kyoto University   大学院農学研究科博士後期課程  

    1997.4 - 2001.3

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  • Kyoto University   大学院農学研究科博士前期課程  

    1995.4 - 1997.3

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  • Kyoto University   農学部   畜産学科

    1991.4 - 1995.3

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Research History

  • Okayama University   The Graduate School of Environmental and Life Science   Associate Professor

    2012.4

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  • Okayama University   The Graduate School of Natural Science and Technology   Associate Professor

    2010.7 - 2012.3

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  • 京都大学大学院   助手

    2001.4 - 2003.3

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Professional Memberships

Committee Memberships

  • 肉用牛研究会   評議員  

    2024.4   

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    Committee type：Academic society

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  • 第72回関西畜産学会   実行委員  

    2022.1 - 2022.12   

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  • 関西畜産学会   会計  

    2017.4 - 2019.3   

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  • 日本動物遺伝育種学会   会計  

    2010.7 - 2012.9   

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  • 日本動物遺伝育種学会   会計  

    2001.4 - 2002.3   

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Papers

  • Presence of a Deletion Mutation of Myostatin (MSTN) Gene Associated With Double‐Muscling Phenotype in Japanese Black Cattle Population

    Nu Anh Thu Le, Rena Kubo, Liushiqi Borjigin, Takayuki Ibi, Shinji Sasaki, Tetsuo Kunieda

    Animal Science Journal   96 ( 1 )   2025.1

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    ABSTRACT

    Mutations in the bovine myostatin (MSTN) gene have been identified as the causative factor for the double‐muscling phenotype in several European cattle breeds, including Belgian Blue, Piedmontese, and Shorthorn. In Japan, following the Meiji Restoration, several European breeds, including Shorthorn, Brown Swiss, Devon, Simmental, and Ayrshire, were introduced and crossbred with native cattle to develop modern Japanese beef cattle breeds, such as Japanese Black cattle. Historical records regarding the breeding of Japanese Black cattle indicate that the double‐muscling phenotype, referred to as “Butajiri,” occasionally appeared in Japanese Black cattle population. These historical observations suggest the potential presence of MSTN gene mutation in the Japanese Black cattle population. The aim of this study was, therefore, to investigate the presence of MSTN gene mutation in the current Japanese Black cattle population. Through screening 400 reproductive females, we identified one cow carrying an 11‐bp deletion in the MSTN gene. While further investigation of the animals in the pedigree of this cow could not reveal any living animals with this mutation, this is the first report demonstrating the presence of the MSTN mutation in the Japanese Black cattle population.

    DOI： 10.1111/asj.70055

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  • Association of a non-synonymous variant of MLH3 gene involved in meiotic recombination with conception rate of Japanese Black cattle

    Thu Nu Anh Le, Trung Ba Nguyen, Takehito Tsuji, Takayuki Ibi, Shinji Sasaki, Tetsuo Kunieda

    Animal Production Science   64 ( 17 )   2024.11

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    Publishing type：Research paper (scientific journal)   Publisher：CSIRO Publishing  

    Context Conception rate, which is an important parameter to evaluate female fertility, has been gradually decreasing in Japanese Black cattle during the past decades. Meiosis is an essential biological process in gamete formation and meiotic failure could be a cause of infertility or reduced fertility in mammals. Of note, single nucleotide polymorphisms (SNPs) in the MLH3 gene involved in meiotic recombination were reported to affect the genome-wide recombination rate of meiosis in cattle. Aims The present study was conducted to investigate the association of SNPs in the MLH3 gene with conception rate in Japanese Black cattle. Methods On the basis of the reproductive data of 2045 Japanese Black cattle born from 1990 to 2009, we selected two groups of the reproductive females with high conception rate (n = 103) and low conception rate (n = 109). Then, we genotyped the SNPs in MLH3 gene in both reproductive groups by sequencing and polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). The significant association of SNPs with conception rate in Japanese Black cattle was estimated by Fisher’s exact test, by using R commander. Key results We found the presence of five SNPs of MLH3 gene in Japanese Black cattle, including non-synonymous variants of MLH3 N408S that has been reported to be involved in meiotic recombination rate and MLH3 S591G newly identified in the present study. Comparison of genotype distributions and allele frequencies of the MLH3 N408S between high and low conception-rate groups indicated a significant difference between these groups, suggesting that this SNP is associated with conception rate. However, there is no significant difference between MLH3 S591G with conception rate in Japanese Black cattle. Conclusions We found a significant association between a non-synonymous variant of MLH3 N408S and conception rate, suggesting that this SNP can be a potential marker for selecting the Japanese Black cattle for improving fertility. We also identified a novel non-synonymous variant of MLH3 S591G in Japanese Black cattle. Implications These findings will be informative for future marker-assisted selection to improve the fertility of Japanese Black cattle. This is the first report of a genetic variant implicated in meiotic recombination being linked to female fertility in cattle.

    DOI： 10.1071/an23418

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  • A genome-wide association study reveals a quantitative trait locus for calf mortality on chromosome 9 in Japanese Black cattle Reviewed

    S. Sasaki, T. Ibi

    ANIMAL GENETICS   52 ( 2 )   214 - 216   2021.4

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    DOI： 10.1111/age.13048

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  • Identification of deleterious recessive haplotypes and candidate deleterious recessive mutations in Japanese Black cattle Reviewed

    Shinji Sasaki, Toshio Watanabe, Takayuki Ibi, Kiyotoshi Hasegawa, Yoichi Sakamoto, Shunsuke Moriwaki, Kazuhito Kurogi, Atsushi Ogino, Takanori Yasumori, Hiroyuki Wakaguri, Eiji Muraki, Youko Miki, Yuichi Yoshida, Yoshinobu Inoue, Ichiro Tabuchi, Ken Iwao, Taichi Arishima, Keisuke Kawashima, Manabu Watanabe, Sumio Sugano, Yoshikazu Sugimoto, Yutaka Suzuki

    SCIENTIFIC REPORTS   11 ( 1 )   2021.3

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    DOI： 10.1038/s41598-021-86225-y

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  • A 44-kb deleted-type copy number variation is associated with decreasing complement component activity and calf mortality in Japanese Black cattle Reviewed

    Shinji Sasaki, Youko Miki, Takayuki Ibi, Hiroyuki Wakaguri, Yuichi Yoshida, Yoshikazu Sugimoto, Yutaka Suzuki

    BMC GENOMICS   22 ( 1 )   2021.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s12864-021-07415-6

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  • Genetic variants of RNF212 involved in meiotic recombination rate and its relation with conception rate in Japanese Black cattle Reviewed

    Thu Nu Anh LE, Trung Ba NGUYEN, Ripon Chandra PAUL, Yu OKUDA, Takehito TSUJI, Takayuki IBI, Shinji SASAKI, Tetsuo KUNIEDA

    The Journal of Animal Genetics   49 ( 1 )   13 - 17   2021

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    Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Animal Breeding and Genetics  

    DOI： 10.5924/abgri.49.13

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  • Genetic characterization of Kushum horses in Kazakhstan based on haplotypes of mtDNA and Y chromosome, and genes associated with important traits of the horses Reviewed

    Trung B. NGUYEN, Ripon C. PAUL, Yu OKUDA, Thu N. A. LE, Phuong T. K. PHAM, Kushaliye J. KAISSAR, Akhmedenov KAZHMURAT, Sarsenova BIBIGUL, Meirat BAKHTIN, Polat KAZYMBET, Suleimenov Zh MARATBEK, Alikhan MELDEBEKOV, Masahide NISHIBORI, Takayuki IBI, Takehito TSUJI, Tetsuo KUNIEDA

    Journal of Equine Science   31 ( 3 )   35 - 43   2020

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    Publishing type：Research paper (scientific journal)   Publisher：Japan Society of Equine Science  

    DOI： 10.1294/jes.31.35

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  • Loss of maternal ANNEXIN A10 via a 34-kb deleted-type copy number variation is associated with embryonic mortality in Japanese Black cattle Reviewed

    Shinji Sasaki, Takayuki Ibi, Takayuki Akiyama, Moriyuki Fukushima, Yoshikazu Sugimoto

    BMC GENOMICS   17 ( 1 )   968   2016.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s12864-016-3312-z

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  • Allelic frequencies and association with carcass traits of six genes in local subpopulations of Japanese Black cattle Reviewed

    Takahiro Nishimaki, Takayuki Ibi, Siqintuya, Naohiko Kobayashi, Tamako Matsuhashi, Takayuki Akiyama, Emi Yoshida, Kazumi Imai, Mayu Matsui, Keiichi Uemura, Hisayoshi Eto, Naoto Watanabe, Tatsuo Fujita, Yosuke Saito, Tomohiko Komatsu, Hiroshi Hoshiba, Hideyuki Mannen, Shinji Sasazaki, Tetsuo Kunieda

    ANIMAL SCIENCE JOURNAL   87 ( 4 )   469 - 476   2016.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/asj.12453

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  • A genome-wide association study reveals a quantitative trait locus for days open on chromosome 2 in Japanese Black cattle Reviewed

    Shinji Sasaki, Takayuki Ibi, Takatoshi Kojima, Yoshikazu Sugimoto

    ANIMAL GENETICS   47 ( 1 )   102 - 105   2016.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/age.12360

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  • Genetic variants in the upstream region of activin receptor IIA are associated with female fertility in Japanese Black cattle Reviewed

    Shinji Sasaki, Takayuki Ibi, Tamako Matsuhashi, Kenji Takeda, Shogo Ikeda, Mayumi Sugimoto, Yoshikazu Sugimoto

    BMC GENETICS   16   123   2015.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s12863-015-0282-0

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  • Genetic parameters of postnatal mortality and birth weight in Japanese Black calves Reviewed

    Takayuki Ibi, Alexander K. Kahi, Hiroyuki Hirooka

    ANIMAL SCIENCE JOURNAL   86 ( 1 )   25 - 30   2015.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/asj.12233

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  • A genome-wide association study reveals a quantitative trait locus for age at first calving in delta/notch-like EGF repeat containing on chromosome 2 in Japanese Black cattle Reviewed

    Shinji Sasaki, Takayuki Ibi, Shogo Ikeda, Yoshikazu Sugimoto

    ANIMAL GENETICS   45 ( 2 )   285 - 287   2014.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/age.12109

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  • Multiple marker effects of single nucleotide polymorphisms in three genes, AKIRIN2, EDG1 and RPL27A, for marbling development in Japanese Black cattle Reviewed

    Shin Sukegawa, Takeshi Miyake, Takayuki Ibi, Yoichi Takahagi, Hiroshi Murakami, Fumiki Morimatsu, Takahisa Yamada

    ANIMAL SCIENCE JOURNAL   85 ( 3 )   193 - 197   2014.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/asj.12108

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  • The assessment of genetic diversity within and among the eight subpopulations of Japanese Black cattle using 52 microsatellite markers Reviewed

    Takahiro Nishimaki, Takayuki Ibi, Yoshihiro Tanabe, Yoshiyuki Miyazaki, Naohiko Kobayashi, Tamako Matsuhashi, Takayuki Akiyama, Emi Yoshida, Kazumi Imai, Mayu Matsui, Keiichi Uemura, Naoto Watanabe, Tatsuo Fujita, Yosuke Saito, Tomohiko Komatsu, Takahisa Yamada, Hideyuki Mannen, Shinji Sasazaki, Tetsuo Kunieda

    ANIMAL SCIENCE JOURNAL   84 ( 8 )   585 - 591   2013.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/asj.12045

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  • Variants in the 3 ' UTR of General Transcription Factor IIF, polypeptide 2 affect female calving efficiency in Japanese Black cattle Reviewed

    Shinji Sasaki, Takayuki Ibi, Toshio Watanabe, Tamako Matsuhashi, Shogo Ikeda, Yoshikazu Sugimoto

    BMC GENETICS   14   41   2013.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/1471-2156-14-41

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  • Genetic relationship of body measurement traits at early age with carcass traits in Japanese black cattle Reviewed

    Tahmina Munim, Takuro Oikawa, Takayuki Ibi, Tetsuo Kunieda

    ANIMAL SCIENCE JOURNAL   84 ( 3 )   206 - 212   2013

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/asj.12005

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  • Genetic variability of maternal effect on body measurements and its intra- and inter-genetic relationship with direct effect in Japanese Black calves Reviewed

    Tahmina Munim, Takuro Oikawa, Takayuki Ibi, Tetsuo Kunieda, Md. Azharul Hoque

    ANIMAL SCIENCE JOURNAL   83 ( 3 )   199 - 206   2012

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1740-0929.2011.00936.x

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  • Optimal culling strategy in relation to biological and economic efficiency and annualized net revenue in the Japanese Black cow–calf production system

    OISHI K., IBI T., KAHI A. K., HIROOKA H.

    The Journal of Agricultural Science   149 ( 6 )   783 - 799   2011.12

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    Language：English   Publisher：Cambridge University Press  

    The objective of the present study was to determine the optimal culling strategy in relation to biological and economic efficiency (BE and EE, respectively) and annualized net revenue (AN) in the Japanese Black cow–calf production system with special reference to the beef quality of culled cows. The herd model focused on two ways of mating: one-mating trial system (ONE) and continuous-mating trial system (CON). ONE assumed that heifers that fail to conceive are culled and cows that fail to conceive are culled at weaning of their calves, while CON assumed that mating continues until all females theoretically conceive. Least square means of carcass data of Japanese Black cows collected from a cooperative farm in Japan were used to estimate the carcass price of a cow by parity and Beef Marbling Standard (BMS) number. The simulation, assuming the current production situation in Japan, indicated that sales of culled cows accounted for 0·10–0·20 of total sales and was an important element in total production. Comparisons between ONE and CON showed that production efficiency in the current situation is higher in CON. The BE, EE and AN were higher in CON than in ONE. The two economic indicators were less sensitive to changes in annual discount rate but highly sensitive to changes in female calf price and BMS number of cows, indicating the importance of considering fluctuations in calf price and potential quality of culled cows' carcasses when estimating the economically optimal parity of culling. The three indicators derived different optimal solutions even in the same mating trial systems, stressing the importance of choice of production indicators when determining the culling strategy and evaluating animal production.

    DOI： 10.1017/S0021859611000347

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  • The use of link provider data to improve national genetic evaluation across weakly connected subpopulations. Reviewed

    Nakaoka H, Gaillard C, Fujinaka K, Watanabe N, Ito M, Kawada K, Ibi T, Sasae Y, Sasaki Y

    Journal of Animal Science   87 ( 1 )   62 - 71   2009

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    DOI： 10.2527/jas.2008-0948

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  • Adjusting for heterogeneity of variance for carcass traits affects single and multiple trait selections in genetic evaluation of Japanese Black cattle Reviewed

    Hirofumi Nakaoka, Claude Gaillard, Takayuki Ibi, Yotaro Sasae, Yoshiyuki Sasaki

    ANIMAL SCIENCE JOURNAL   79 ( 6 )   645 - 654   2008.12

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    DOI： 10.1111/j.1740-0929.2008.00576.x

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  • Genetic parameters for gestation length and the relationship with birth weight and carcass traits in Japanese Black cattle Reviewed

    Takayuki Ibi, Alexander K. Kahi, Hiroyuki Hirooka

    ANIMAL SCIENCE JOURNAL   79 ( 3 )   297 - 302   2008.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1740-0929.2008.00530.x

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  • Effectiveness of adjusting for heterogeneity of variance in genetic evaluation of Japanese Black cattle. Reviewed

    Nakaoka H, Narita A, Ibi T, Sasae Y, Miyake T, Yamada T, Sasaki Y

    Journal of Animal Science   85 ( 10 )   2429 - 2436   2007

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Society of Animal Science  

    DOI： 10.2527/jas.2007-0063

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  • Effect of carcass price fluctuations on genetic and economic evaluation of carcass traits in Japanese Black cattle Reviewed

    T. Ibi, A. K. Kahi, H. Hirooka

    Journal of Animal Science   84 ( 12 )   3204 - 3211   2006.12

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    DOI： 10.2527/jas.2005-610

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  • Genotype × environment interaction effects on carcass traits in Japanese Black cattle Reviewed

    T. Ibi, H. Hirooka, A. K. Kahi, Y. Sasae, Y. Sasaki

    Journal of Animal Science   83 ( 7 )   1503 - 1510   2005

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  • Population structure of the Japanese Brown breed Reviewed

    T Ibi, K Moriya, M Matsumoto, S Koba, Y Sasaki

    JOURNAL OF ANIMAL BREEDING AND GENETICS-ZEITSCHRIFT FUR TIERZUCHTUNG UND ZUCHTUNGSBIOLOGIE   114 ( 1 )   43 - 48   1997.2

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    DOI： 10.1111/j.1439-0388.1997.tb00490.x

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Books

  • 動物遺伝育種学

    祝前, 博明, 国枝, 哲夫, 野村, 哲郎, 万年, 英之, 西堀, 正英, 下桐, 猛, 揖斐, 隆之, 石川, 明, 大山, 憲二, 三宅, 武, 古川, 力, 長嶺, 慶隆, 山田, 宜永, 広岡, 博之

    朝倉書店  2017.3  （ ISBN:9784254450309 ）

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    Total pages：vi, 204p   Language：Japanese

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MISC

  • 岡山県固有の黒毛和種希少系統の遺伝学的特徴の解析

    米田一裕, 奥田ゆう, Siqintuya, 西牧孝洋, 松本大和, 宮崎義之, 揖斐隆之, 辻岳人, 国枝哲夫

    日本畜産学会報   87 ( 1 )   1‐10(J‐STAGE) - 10   2016.2

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    Language：Japanese   Publisher：公益社団法人 日本畜産学会  

    The effective population size of Japanese Black cattle has been significantly reduced and maintaining the genetic diversity of the population is important for breeding of Japanese Black cattle. For the conservation of genetic diversity, use of different lines of the breed with unique genetic characteristics for breeding, instead of intensive use of sires of few particular lines as common in current breeding of Japanese Black cattle, will be effective to prevent the genetic homogenization of the population. In the present study, we performed genetic characterization a population of a rare line of Japanese Black cattle, which has been originated from ancestral "Tsuru-ushi" in Okayama prefecture. By using microsatellite markers, allelic richness, and average observed and expected heterozygosity are 3.48, 0.514, and 0.511, respectively, and these values were lower than those of most of the Japanese Black cattle local subpopulations compared. The result of the clustering analyses indicated that the animals of the rare line formed a single group with a cluster that was clearly distinguished from the other populations. Sequence analysis of mitochondrial D-loop region revealed that only two haplotypes were observed in the population and 80% of the animals in the population possess a single haplotype. However, the other haplotype was a novel unique haplotype that has not been reported in cattle. Genotyping of six genes associated with important traits revealed that <i>SREBP1</i> and <i>NCAPG</i> loci were fixed for a single allele in the population and more than 90% of animals possess an allele of <i>MC1R</i> locus. These findings indicated that while genetic diversity of the population of the rare line is lower than those of the Japanese Black cattle local subpopulations, this population has unique genetic characteristics that were distinguished from the other populations and, therefore, the rare line is important for maintaining genetic diversity of Japanese Black cattle.

    DOI： 10.2508/chikusan.87.1

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  • Survey on the undernourished university students who tend to lack breakfast; a proposal for a novel viewpoint for the improvement

    田村隆, 揖斐隆之, 稲垣賢二, 久保康隆, 奥田潔

    岡山大学農学部学術報告(Web)   105 ( 105 )   1-5 (WEB ONLY) - 5   2016.2

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    Language：Japanese   Publisher：岡山大学農学部  

    This study investigated the current status and causes underneath the life of university students who tend to lack breakfast at a relatively high frequency, and statistical analysis on consequences leading to such lack of well-nourished eating habitat in their university life. In October 2014, self-assessed questionnaires were administered to over 150 faculty students. It contained questions about breakfast habits, time allowance for the morning class, and lunchtime setting in their high school timetable. Breakfast states were clearly separated in three groups : 68% of students regularly have breakfast throughout the weekdays, 21% students skipping the breakfast occasionally, and 11% student no habit for breakfast at all. The survey on the high school lives revealed that 70% students used to have lunch 30 min later than the lunchtime set in the university timetable, 7% of them had the lunch time even more than 1 h later. Lunchtime varies among high schools, and statistical significance was revealed (p<0.01) that schools with higher deviation scores tend have late lunch beyond 12: 30. Accordingly, university students were given directions to prepare for the timetable reform on postulation of having lunch time over one o'clock. After continuous survey on the breakfast habits during the second semester, more than 90% of students established the habit of breakfast regularly in their university lives with the improved consciousness toward well-balanced healthy breakfast contents for their higher level of education quality.

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  • 「豊味(うま)いの証」豊後牛肉生産技術の確立―牛肉中のオレイン酸含有率を取り入れた産肉能力育種価手法の検討―

    志村英明, 利光昭彦, 衞藤央好, 安高康幸, 揖斐隆之

    九州農業研究発表会専門部会発表要旨集   78th   71   2015.8

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  • 岡山県のジャージー集団におけるBCO2遺伝子の新たな変異と脂肪淡黄色との関連性

    清水佑起, 石倉健一, 揖斐隆之, 国枝哲夫, 辻岳人

    日本畜産学会大会講演要旨   119th   115   2015.3

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  • オレイン酸含有率を用いた新たな育種価評価の確立

    利光昭彦, 手島久智, 衛藤央好, 三輪友樹, 藤田達男, 揖斐隆之

    九州沖縄農業試験研究の成果情報(Web)   2015   WEB ONLY   2015

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  • Allelic distributions of genes involved in economical traits, hereditary disorder, and coat color in a population of Kuchinoshima cattle

    SIQINTUYA, 西牧孝洋, 揖斐隆之, 辻岳人, 米田一裕, 大島一郎, 片平清美, 万年英之, 下桐猛, 印牧美佐生, 国枝哲夫

    動物遺伝育種研究   42 ( 1 )   11 - 19   2014.7

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    Language：Japanese   Publisher：日本動物遺伝育種学会  

    Kuchinoshima cattle is a breed of the unique feral cattle originated from grazing Japanese native cattle in Kuchinoshima island of Tokara Islands in Kagoshima prefecture during Meiji and Taisho periods. The distribution and frequencies of alleles of the genes associated with economical traits and hereditary disorders have been reported in populations of Japanese Black cattle that is a major beef cattle breed in Japan and Mishima cattle that is another Japanese native cattle breed in Mishima island, but those of Kuchinoshima cattle have not been reported. Therefore, we investigated the allelic distribution and frequencies of the genes associated with economical traits, hereditary disorders, and coat color to reveal genetic characteristic of Kuchinoshima cattle by comparison of the distributions among Kuchinoshima, Japanese Black, and Mishima cattle. By using 32 DNA samples collected from Kuchinoshima cattle, we genotyped <I>NCAPG</I>, <I>FASN</I>, <I>SCD</I>, and <I>SREBP-1</I> genes associated with economical traits including carcass weight and fatty acid composition, <I>F11</I> gene responsible for factor XI deficiency, and <I>MC1R</I> gene involved in coat color determination by PCR or PCR-RFLP assay and compared these results with those of Japanese Black and Mishima cattle. As a result, we found that while both <I>G</I> and <I>T</I> alleles of <I>NCAPG</I> locus were observed in the population of Japanese black cattle, the locus was fixed to <I>T</I> allele in the populations of Kuchinoshima and Mishima cattle, and that while both <I>TW</I> and AR alleles of <I>FASN</I> locus were observed in Japanese black cattle, the locus was fixed to <I>AR</I> allele in Kuchinoshima cattle and <I>TW</I> allele in Mishima cattle. For the <I>SCD</I> and <I>SREBP-1</I> loci, both <I>A</I> and <I>V</I> alleles of <I>SCD</I> locus were observed in Japanese Black, Kuchinoshima, and Mishima cattle, and both <I>S</I> and <I>L</I> alleles <I>SREBP-1</I> locus were observed in Japanese Black and Mishima cattle but fixed to L allele in Kuchinoshima cattle. Furthermore, while both (+) and (-) alleles of <I>F11</I> locus were observed in the population of Japanese black cattle, the locus was fixed to (+) allele in the populations of Kuchinoshima and Mishima cattle. Finally, we observed <I>E</I> <SUP>+</SUP> and e alleles of <I>MC1R</I> locus in Kuchinoshima cattle, while <I>E</I> <SUP>D</SUP> and <I>E</I> <SUP>+</SUP> allele but no e allele in Japanese Black cattle and only <I>E</I> <SUP>+</SUP> allele in Mishima cattle were observed. These findings indicate that Kuchinoshima cattle comprises a population of Japanese native cattle possessing unique genetic characteristics different from those of Japanese Black and Mishima cattle, which has been formed under geographically isolated circumstance with relatively small population without influence of foreign breeds.

    DOI： 10.5924/abgri.42.11

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  • 和牛の胚死滅を引き起こす劣性変異の探索

    佐々木慎二, 渡邊敏夫, 揖斐隆之, 杉本喜憲

    日本畜産学会大会講演要旨   116th   155   2013.3

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  • 黒毛和種の胚死滅を引き起こす劣性変異の探索

    佐々木慎二, 渡邊敏夫, 揖斐隆之, 杉本喜憲

    動物遺伝育種研究   40 ( 2 )   69   2012.12

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  • 黒毛和種における第XI因子欠乏症と異常産,新生仔死との関連

    斯琴図雅, 緒方良彦, 森友靖生, 三浦潔, 西牧孝洋, 揖斐隆之, 国枝哲夫

    動物遺伝育種研究   40 ( 2 )   78   2012.12

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  • 黒毛和種の枝肉形質に対するSCD,SREBP1,FASNおよびNCAPG遺伝子多型の関連解析

    西牧孝洋, 揖斐隆之, 斯琴図雅, 小林直彦, 松橋珠子, 今井佳積, 松井茉由, 上村圭一, 衛藤央好, 渡邉直人, 藤田達男, 齊藤陽介, 小松智彦, 万年英之, 笹崎晋史, 国枝哲夫

    動物遺伝育種研究   40 ( 2 )   71   2012.12

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  • 黒毛和種・雌牛の受胎率を低下させる遺伝的要因の解明

    佐々木慎二, 揖斐隆之, 松橋珠子, 池田省吾, 小林直彦, 杉本喜憲

    日本畜産学会大会講演要旨   115th   166   2012.3

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  • 黒毛和種における各地域集団の遺伝的多様性と類縁関係の解析

    西牧孝洋, 揖斐隆之, 田邉義弘, 宮崎義之, 小林直彦, 松橋珠子, 秋山敬孝, 吉田恵実, 今井佳積, 松井茉由, 上村圭一, 渡邉直人, 藤田達男, 齊藤陽介, 万年英之, 笹崎晋史, 国枝哲夫

    動物遺伝育種研究   39 ( 2 )   142   2011.12

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  • 黒毛和種における雌牛の繁殖能力に関するゲノム解析

    佐々木慎二, 揖斐隆之, 松橋珠子, 池田省吾, 小林直彦, 渡邊敏夫, 杉本喜憲

    動物遺伝育種研究   39 ( 2 )   132   2011.12

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  • Genome-Wide Association Study for Female Reproductive Efficiency in Japanese Black Cattle

    Shinji Sasaki, Takayuki Ibi, Toshio Watanabe, Yoshikazu Sugimoto

    BIOLOGY OF REPRODUCTION   85   2011.7

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  • Estimation of genetic parameters for beef marbling score and marbling on top round of Japanese Black

    揖斐隆之

    岡山大学農学部学術報告   100   61 - 65   2011.2

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    Japanese Black cattle are the predominant beef breed in Japan. This breed has excellent meat quality, especially degree of (intramuscular) marbling. Degree of marbling was measured at the 6th to 7th rib section and according to the Beef Marbling Standard with scores 1 to 12 (so-called BMS), with number 12 being the best. BMS is used with business deal of beef carcass and has strongly effect on selling price of carcass. On the other hands, more degree of marbling on top round (MTR) is better carcass unit price in same BMS, because of good price of part of thigh. MTR has been focused in recent years. However, MTR is unclear how to evaluate, therefore, collection of records and genetic evaluations are not enough. In this study, records of MTR were collected and then, genetic parameters for BMS and MTR of Japanese Black were estimated. The original data were 11,052 carcass records collected from April in 2008 to June in 2009 at the Agura Farm. Only information from abattoirs having more than 20 records and feedlot farms having more than 10 records were used. This resulted in a total of 10,990 records. In this study, pedigree information was traced back to two generations. An animal model that included all relationships among all animals was used as a statistical model. The fixed effects included sex, farm, abattoir and the combination of slaughter year and month. Slaughter age was fitted as linear covariables. Genetic parameters were estimated using REMLF90 and THRGIBBS1F90 programs. The estimates of heritability were moderately high (0.37 to 0.44) for MTR treated as threshold traits. The estimates of phenotypic and genetic correlations between BMS and MTR were very high (0.87 and 0.98, respectively). These indicate that MTR can be improved genetically and improvement of MTR can treat BMS as indicator.

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  • 黒毛和午におけるBMSおよびモモヌケの遺伝的パラメータの推定

    揖斐 隆之

    岡山大學農學部學術報告 = Scientific report of the Faculty of Agriculture, Okayama University   ( 100 )   61 - 65   2011.2

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  • 黒毛和種における雌牛の繁殖能力に関するゲノム解析

    佐々木慎二, 揖斐隆之, 渡邊敏夫, 松橋珠子, 中藤由紀, 小林直彦, 杉本喜憲

    動物遺伝育種研究   38 ( 2 )   131   2010.12

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  • 3.黒毛和牛におけるBMSおよびモモヌケの遺伝的パラメータの推定

    揖斐隆之

    肉用牛研究会報   88 ( 88 )   67 - 68   2010.1

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  • 黒毛和牛におけるBMSおよびモモヌケの遺伝的パラメータの推定

    揖斐 隆之

    肉用牛研究会報   88   67 - 68   2010.1

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  • 性判別技術や双子生産技術の導入が黒毛和種繁殖生産の経済性に及ぼす影響

    大石風人, 揖斐隆之, 熊谷元, 広岡博之

    日本畜産学会大会講演要旨   110th   158   2009.3

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  • 安愚楽共済牧場における家畜育種の取り組み

    揖斐隆之

    動物遺伝育種研究   36 ( 1 )   29 - 37   2008.6

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    DOI： 10.5924/abgri2000.36.29

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  • 繁殖雌牛の経産回数と繁殖性,割引率の変化が和牛繁殖生産の経済性に及ぼす影響

    大石風人, 揖斐隆之, 熊谷元, 広岡博之

    日本畜産学会大会講演要旨   109th   95   2008.3

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  • 黒毛和種の子牛における初期の生存性に関する遺伝的パラメータの推定

    揖斐隆之, 広岡博之

    日本畜産学会大会講演要旨   109th   95   2008.3

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  • Breeding programs of Japanese Black cattle in Agura Farm

    IBI Takayuki

    The journal of animal genetics   36 ( 1 )   29 - 37   2008

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    Publisher：Japanese Society of Animal Breeding and Genetics  

    DOI： 10.5924/abgri2000.36.29

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  • 黒毛和種における広域種牛評価の精度に関する研究

    中岡博史, 藤中邦則, 渡邉直人, 川田啓介, 揖斐隆之, 佐々江洋太郎, 佐々木義之

    動物遺伝育種研究   35 ( 2 )   232   2007.12

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  • 和牛繁殖生産における繁殖雌牛の供用年数と繁殖率の変化が生産効率に及ぼす影響

    大石風人, 揖斐隆之, 熊谷元, 広岡博之

    日本畜産学会大会講演要旨   108th   38   2007.9

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  • 肉用牛経営の個別属性や経営意識が家畜排泄物の処理・利用に及ぼす影響

    長命洋佑, 揖斐隆之, 仙田徹志, 森佳子, 広岡博之

    農林業問題研究   43 ( 1 )   51 - 56   2007.6

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    Language：Japanese   Publisher：地域農林経済学会  

    DOI： 10.7310/arfe1965.43.51

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  • 家畜育種における遺伝と環境の交互作用

    揖斐隆之, 広岡博之

    動物遺伝育種研究   35 ( 1 )   25 - 31   2007.6

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    DOI： 10.5924/abgri2000.35.25

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  • 黒毛和種の妊娠期間に関する遺伝的パラメータの推定と生時体重や枝肉形質との遺伝的関連性

    揖斐隆之, 広岡博之

    日本畜産学会大会講演要旨   107th   64   2007.3

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  • 肉用牛経営における潜在的窒素負荷量が家畜排泄物の処理・利用の意向に及ぼす影響

    長命洋佑, 揖斐隆之, 熊谷元, 広岡博之

    日本畜産学会大会講演要旨   107th   156   2007.3

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  • 肉用牛経営の個別属性や経営意識が家畜排泄物の処理・利用に及ぼす影響

    長命 洋佑, 揖斐 隆之, 仙田 徹志, 森 佳子, 広岡 博之

    農林業問題研究   43 ( 1 )   51 - 56   2007

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    DOI： 10.7310/arfe1965.43.51

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  • Genotype × environment interaction for animal breeding

    IBI Takayuki, HIROOKA Hiroyuki

    The journal of animal genetics   35 ( 1 )   25 - 31   2007

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    DOI： 10.5924/abgri2000.35.25

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  • 和牛繁殖生産における繁殖雌牛の供用年数と生産効率との関係

    大石風人, 熊谷元, 揖斐隆之, 広岡博之

    システム農学会シンポジウム要旨集   2007   22 - 23   2007

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  • 黒毛和種集団の広域種牛評価によるサブクラス間不均一分散の補正の有効性

    中岡博史, 三宅武, 揖斐隆之, 佐々江洋太郎, 山田宜永, 佐々木義之

    動物遺伝育種研究   34 ( 2 )   119   2006.12

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  • Effects of Attributes and Consciousness of Fattening Farms Households on Carcass Prices and Traits

    CHOUMEI Yousuke, IBI Takayuki, HIROOKA Hiroyuki

    Nihon Chikusan Gakkaiho   77 ( 2 )   289 - 294   2006.5

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    The objective of this study was to investigate effects of attributes and consciousness of farm households on prices and traits of carcass sold from the farms. Questionnaire surveys were conducted to farms under the Agura Co-operative Farm and 91 questionnaires were available for the present analysis. Best linear unbiased estimations (BLUEs) of each farm for carcass prices and traits were estimated using 20,346 carcass records collected from January 2004 to March 2005 at the Agura co-operation Farm. The BLUEs were analyzed by least squares procedures using a model containing attributes and consciousness of farm households obtained from questionnaires as sources of variation. The result showed that farm households who make great account of both income and joy of life in keeping animals can produce carcasses with higher quality. In addition, it was shown that the effect of farm size on carcass unit price was quadratic ; as farm size increased, carcass unit price decreased and increased beyond the size of more than 932 heads.

    DOI： 10.2508/chikusan.77.289

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  • 黒毛和種牛の産肉性に関する選抜指数の設定

    DORONBEKOV Kanat, 揖斐隆之, 佐々江洋太郎, 三宅武, 佐々木義之

    日本畜産学会大会講演要旨   106th   58   2006.3

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  • Gibbs Samplingによる黒毛和種枝肉におけるかしの遺伝率推定

    揖斐隆之, 三宅武, 佐々木義之

    肉用牛研究会報   ( 80 )   69 - 73   2005.12

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  • Estimation of heritability for defects of beef carcass using Gibbs sampling in Japanese Black cattle

    IBI Takayuki, MIYAKE Takeshi, SASAKI Yoshiyuki

    Bulletin of Beef Cattle Science   80   69 - 73   2005.12

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  • 枝肉形質間の遺伝相関係数に基づく肉牛品種間系統樹の作成ならびに遺伝的距離の検討

    三宅武, 中岡博史, 小谷基, 広岡博之, 小邦朋子, 住尾善彦, 松本道夫, 伊藤雅之, 倉原貴美, 揖斐隆之, 佐々江洋太郎, 藤中邦則, 太田垣進, 道後泰治, 佐々木義之

    肉用牛研究会報   80 ( 80 )   23 - 25   2005.12

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  • 黒毛和種集団の全国的な種牛評価におけるサブクラス間の不均一分散の影響~不均一分散が複数形質を考慮した総合評価に及ぼす影響

    中岡博史, 揖斐隆之, 佐々江洋太郎, 佐々木義之

    肉用牛研究会報   80 ( 80 )   4 - 6   2005.12

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  • 黒毛和牛における枝肉価格に関する遺伝的パラメータのBSEの発生による変遷

    揖斐隆之, 広岡博之

    日本畜産学会大会講演要旨   105th   57   2005.8

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  • 全国的な黒毛和種集団の種牛評価における不均一分散の補正

    中岡博史, 揖斐隆之, 佐々江洋太郎, 佐々木義之

    日本畜産学会大会講演要旨   105th   52   2005.8

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  • サブクラス間の分散成分不均一性を考慮した種牛評価法とその予測育種価への影響

    中岡博史, 揖斐隆之, 佐々江洋太郎, 佐々木義之

    日本畜産学会大会講演要旨   104th   43   2005.3

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  • Gibbs Samplingによる黒毛和牛枝肉におけるかしの遺伝率推定

    揖斐隆之, 三宅武, 佐々江洋太郎, 佐々木義之

    日本畜産学会大会講演要旨   104th   84   2005.3

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  • 枝肉形質間の遺伝相関係数に基づく肉牛品種間系統樹の作成ならびに遺伝的距離の検討

    三宅武, 中岡博史, 小谷基, 広岡博之, 小邦朋子, 住尾善彦, 松本道夫, 伊藤雅之, 倉原貴美, 揖斐隆之, 佐々江洋太郎, 藤中邦則, 太田垣進, 道後泰治, 佐々木義之

    肉用牛研究会大会   43rd   23 - 26   2005

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  • 黒毛和種集団の全国的な種牛評価におけるサブクラス間の不均一分散の影響~不均一分散が複数形質を考慮した総合評価に及ぼす影響

    中岡博史, 揖斐隆之, 佐々江洋太郎, 佐々木義之

    肉用牛研究会大会   43rd   1 - 4   2005

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  • 黒毛和種における全国的な種牛評価の可能性および数学モデルに関する研究

    小谷基, 中岡博史, 成田暁, 揖斐隆之, 佐々江洋太郎, 佐々木義之

    日本畜産学会報   75 ( 3 )   353 - 361   2004.8

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    Comparisons of breeding stock among the Japanese Black cattle across regions were impeded because breeding programs and genetic evaluations were carried out primarily within regions. A nationwide connectedness was missing. The objective of this study was to investigate the possibility of a national genetic evaluation of breeding stocks based on appropriate mathematical models and suited material. Goodness of fit of the models was measured by the prediction error variance, the Akaike’s information criterion and coefficient of determination. The data consisted of field records on carcass traits (beef marbling standard number, BMS No. ; carcass weight, CW ; rib-eye area, REA ; rib thickness, RT ; subcutaneous fat thickness, SFT) of fattened Japanese Black cattle collected from 1997 to 2002 among the nationwide fattening farms of Agura Kyosai Bokujo, Inc. (N=49,810). The pedigree of those animals was surveyed, too. More than 95% of all possible pairs of fattening farms have beef animals with common sires. Further beef animals from different prefectures were dispersed over fattening farms so that comparisons among all prefectures were possible. Six mathematical models were applied, which differed in the way how each of the fixed effects was taken into account. The estimated heritabilities do not vary significantly among the applied mathematical models (h<SUP>2</SUP> for BMS No. : 0.43 ; CW : 0.45-0.49 ; REA : 0.32-0.33 ; RT : 0.31-0.33 ; SFT : 0.46-0.47). The models with the best goodness of fit are obtained when all the fixed effects are taken into account as a combination effect or when all the fixed effects but the effect of sex are taken into account as a combination of them. We were able to demonstrate that the genetic connectedness among subpopulations is sufficient in the involved fattening farms in order to establish a genetic evaluation on a national scale. A nationwide evaluation of the Japanese Black breeding stocks will enhance the genetic improvement of the breed by a more efficient selection of excellent sires and dams.

    DOI： 10.2508/chikusan.75.353

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  • 黒毛和種および褐毛和種の枝肉形質に関する遺伝性の類似

    三宅武, 揖斐隆之, 松本道夫, 藤中邦則, 山田宜永, 佐々木義之

    日本畜産学会大会講演要旨   103rd   137   2004.3

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  • 黒毛和種の枝肉形質における遺伝と管理システムの交互作用に関する研究

    揖斐隆之, 広岡博之, 佐々江洋太郎, 佐々木義之

    日本畜産学会大会講演要旨   103rd   138   2004.3

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  • Gibbs Samplingによる競走馬における上喉頭片麻痺の遺伝率推定

    揖斐 隆之, 三宅 武, 帆保 誠二

    馬の科学   41 ( 3 )   155 - 162   2004

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  • 黒毛和種の枝肉形質における遺伝と地域の交互作用に関する研究

    揖斐隆之, 広岡博之, 佐々江洋太郎, 佐々木義之

    日本畜産学会大会講演要旨   102nd   39   2003.9

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  • 黒毛和種における全国的な種牛評価に関する研究 その1 遺伝的結合度の検討と数学モデルの遺伝率推定値への影響

    中岡博史, 小谷基, 成田暁, 揖斐隆之, 佐々江洋太郎, 佐々木義之

    日本畜産学会大会講演要旨   102nd   39   2003.9

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  • 黒毛和種における全国的な種牛評価に関する研究 その2 最適な数学モデルに関する検討

    小谷基, 中岡博史, 成田暁, 揖斐隆之, 佐々江洋太郎, 佐々木義之

    日本畜産学会大会講演要旨   102nd   39   2003.9

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  • Estimation of heritability of laryngeal Hemiplegia in the Thoroughbred horse by gibbs sampling

    Takayuki Ibi, Takeshi Miyake, Seiji Hobo, Hironori Oki, Nobushige Ishida, Yoshiyuki Sasaki

    Journal of Equine Science   14 ( 3 )   81 - 86   2003.9

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  • Estimation of heritability of laryngeal Hemiplegia in the Thoroughbred horse by gibbs sampling

    Takayuki Ibi, Takeshi Miyake, Seiji Hobo, Hironori Oki, Nobushige Ishida, Yoshiyuki Sasaki

    Journal of Equine Science   14 ( 3 )   81 - 86   2003.9

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    DOI： 10.1294/jes.14.81

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  • Genetic Statistical Analysis on Carcass Traits and Daily Gain of Japanese Black Cattle in Southern Part of Iwate Prefecture

    川田啓介, 兼松重任, 黒沢弥悦, 揖斐隆之, 佐々木義之

    日本畜産学会報   74 ( 2 )   187 - 193   2003.5

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    In order to elucidate the properties for the breeding of Japanese Black Cattle population in the southern part of Iwate prefecture, genetic parameters, breeding values and genetic trends of carcass traits and daily gain (DG) were estimated using 3,467 field records from the steers and heifers fattened in Maesawa, Iwate prefecture, from 1994 to 1999. Only farmers who shipped more than 30 animals during this period were chosen. It was remarkably noticed that best linear unbiased estimations for beef marbling standard number (BMS) have decreased successively for years. The heritability estimates for carcass traits and DG were as follows : 0.66 for BMS, 0.51 for DG, 0.64 for carcass weight, 0.36 for rib-eye area, 0.25 for rib thickness and 0.33 for subcutaneous fat thickness. The genetic correlation, between BMS and other traits were as follows : 0.1 for DG, 0.18 for the carcass weight, 0.48 for the rib-eye area, 0.37 for the rib thickness and -0.24 for the subcutaneous fat thickness. As far as the genetic trends were concerned, improvements on the BMS and the subcutaneous fat thickness were highly regarded, while the carcass weight was rarely taken into consideration since 1991. On the other hand, the phenotypic average for BMS of animals fattened in Maesawa has tended to decrease since 1991. Based on the present results, factors other than genetic improvement could be considered the cause of the decrease.

    DOI： 10.2508/chikusan.74.187

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  • 黒毛和種における全国的な種牛評価のための数学モデルに関する予備的検討

    小谷基, 成田暁, 揖斐隆之, 佐々江洋太郎, 佐々木義之

    日本畜産学会大会講演要旨   101st   107   2003.3

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  • 遺伝子発現量の多サンプル間での比較のためのディファレンシャルディスプレイ法に関する検討

    永田幸洋, 揖斐隆之, 谷口幸雄, 山田宜永, 佐々木義之

    動物遺伝育種研究   30 ( 1 )   11 - 18   2002.11

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  • Gibbs samplingにより混合遺伝子モデルを用いたQTL遺伝子型推定法の検討

    大野涼太, 成田暁, 揖斐隆之, 佐々木義之

    日本畜産学会大会講演要旨   100th   108   2002.3

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  • 非近交系集団における混合遺伝モデルを用いた複数のQTL解析法に関する研究 RJMCMCを応用したQTL数の推定

    成田暁, 大野涼太, 揖斐隆之, 佐々木義之

    日本畜産学会大会講演要旨   100th   109   2002.3

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  • Association Analysis between the Deletion Mutant Allele of Claudin‐16 Deficiency and Carcass Traits in Japanese Black Cattle.

    小林直彦, 平野貴, 揖斐隆之, 大谷健, 杉本喜憲

    Animal Science Journal   73 ( 1 )   19 - 23   2002.2

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    Language：Japanese   Publisher：公益社団法人 日本畜産学会  

    <i>Claudin-16</i> (<i>CL-16</i>) deficiency is an autosomal recessive disease in Japanese black cattle. The association analysis between the deletion mutant allele (<i>d</i>) of <i>CL-16</i> deficiency and carcass traits was investigated. Normal allele (<i>D</i>) and mutant allele (<i>d</i>) were diagnosed in 464 fattened progeny of three carrier sires (<i>Dd</i>), and 140 cows of Sire 1 whose common ancestor was revealed as <i>Dd</i>. The progeny were composed of 230 normal (<i>DD</i>) and 234 carrier (<i>Dd</i>). The cows were composed of 60 <i>DD</i> and 80 <i>Dd</i>. The association analysis was carried out among the <i>CL-16</i> genotypes, and carcass traits and predicted breeding value for carcass traits using the 464 progeny and 140 cows, respectively. Analysis of variance was performed considering the <i>CL-16</i> genotypes, sire, sex, farm and age (linear and quadratic) as the source of variance in order to evaluate factors affecting carcass traits. Significant differences in BMS number, carcass weight, area of rib eye, and rib thickness were observed between the sires. Significant differences in carcass weight were observed in the influence of sex. Significant differences in BMS number, carcass weight, and rib thickness were observed between farms. The regression of any carcass traits on final age (linear and quadratic) was not statistically significant. The significant association of the <i>CL-16</i> genotypes were not seen in the carcass traits after the effects of environmental factors were eliminated. Moreover, a significant association of the genotypes was not seen (P>0.05) with predicted breeding value for carcass traits in cows of Sire-1. Furthermore, these carcass traits had no significant linkage with the approximately 30 cM region including <i>CL-16</i> locus in a half-sib family of Sire-1 comprising 136 offspring (P>0.05). Therefore, exclusion of the mutant allele <i>d</i> from Japanese Black cattle population would not deteriorate the carcass traits.

    DOI： 10.2508/chikusan.73.19

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  • Differential display method used for the comparison of mRNA expression level among many samples

    NAGATA Yukihiro, IBI Takayuki, TANIGUCHI Yukio, YAMADA Takahisa, SASAKI Yoshiyuki

    The journal of animal genetics   30 ( 1 )   11 - 18   2002

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    A reproducibility of experimental data is important for application of differential display method to the comparison of mRNA expression level among many samples. First, we developed the optimum method to evaluate size (BP) and yield (IOD) of PCR products by analyzing an electrophoretic image. BP (A), which is a way to calculate size of each product by using 3 molecular markers loaded in extreme and central lanes of gel as references, was optimal for size evaluation. Further, IOD (A) and IOD (P), respectively, which are way to use a measured absolute value of signal intensity of each product, and to use a relative value of signal intensity of each product as proportion of total sum of all products in an identical lane, as yield, were determined as the suboptimum and optimum evaluation methods. Next, we performed an analysis of variance of intergel, interlane, and interreaction effects, when using the optimum method. We detected statistically significant effects for intergel and interlane, but not for interreaction, when using BP (A) method. As for IOD (A) and IOD (P), significant effects for intergel and interreaction were observed, but not for interlane. Finally, we evaluated the replicate pattern that is suitable for a largescale gene expression profiling, by examining coefficient of variation of combined effects for BP and IOD obtained by 4 different replicate patterns.

    DOI： 10.5924/abgri2000.30.11

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  • Association Analysis between the Deletion Mutant Allele of Claudin-16 Deficiency and Carcass Traits in Japanese Black Cattle

    KOBAYASHI Naohiko, HIRANO Takashi, IBI Takayuki, OHTANI Tuyoshi, SUGIMOTO Yoshikazu

    Nihon Chikusan Gakkaiho   73 ( 1 )   19 - 23   2002

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    Language：Japanese   Publisher：Japanese Society of Animal Science  

    Claudin-16 (CL-16) deficiency is an autosomal recessive disease in Japanese black cattle. The association analysis between the deletion mutant allele (d) of CL-16 deficiency and carcass traits was investigated. Normal allele (D) and mutant allele (d) were diagnosed in 464 fattened progeny of three carrier sires (Dd), and 140 cows of Sire 1 whose common ancestor was revealed as Dd. The progeny were composed of 230 normal (DD) and 234 carrier (Dd). The cows were composed of 60 DD and 80 Dd. The association analysis was carried out among the CL-16 genotypes, and carcass traits and predicted breeding value for carcass traits using the 464 progeny and 140 cows, respectively. Analysis of variance was performed considering the CL-16 genotypes, sire, sex, farm and age (linear and quadratic) as the source of variance in order to evaluate factors affecting carcass traits. Significant differences in BMS number, carcass weight, area of rib eye, and rib thickness were observed between the sires. Significant differences in carcass weight were observed in the influence of sex. Significant differences in BMS number, carcass weight, and rib thickness were observed between farms. The regression of any carcass traits on final age (linear and quadratic) was not statistically significant. The significant association of the CL-16 genotypes were not seen in the carcass traits after the effects of environmental factors were eliminated. Moreover, a significant association of the genotypes was not seen (P>0.05) with predicted breeding value for carcass traits in cows of Sire-1. Furthermore, these carcass traits had no significant linkage with the approximately 30 cM region including CL-16 locus in a half-sib family of Sire-1 comprising 136 offspring (P>0.05). Therefore, exclusion of the mutant allele d from Japanese Black cattle population would not deteriorate the carcass traits.

    DOI： 10.2508/chikusan.73.19

    DOI： 10.1292/jvms.68.319_references_DOI_3Tfx6jEYktXsr2H9WH83lLPf6qK

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    Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I6266493

  • 多サンプル間での遺伝子発現量の比較に適用可能な半定量的デイファレンシャルディスプレイ法の確立

    永田幸洋, 揖斐隆之, 谷口幸雄, 山田宜永, 佐々木義之

    日本畜産学会大会講演要旨   99th   46   2001.8

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  • Gibbs Samplingによる競走馬における上部気道疾患の遺伝率の推定

    揖斐隆之, 三宅武, 楠瀬良, 帆保誠二, 沖博憲, 佐々木義之

    日本畜産学会大会講演要旨   99th   45   2001.8

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  • 近交係数の上昇を抑え遺伝的改良を最大にする育種計画

    揖斐隆之, 佐々木義之

    日本畜産学会大会講演要旨   98th   93   2001.3

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  • ラット最長筋内脂肪量の組織化学的測定法の確立ならびにOLETF,F344およびそれらのF1間の比較

    田之村秀樹, 三宅武, 谷口幸雄, 揖斐隆之, 真鍋昇, 山田宜永, 佐々木義之

    日本畜産学会大会講演要旨   97th   61   2000.3

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  • 近交係数の上昇を抑え遺伝的改良を最大にする育種システム

    揖斐隆之, 守田智, 佐々木義之

    日本畜産学会大会講演要旨   96th   67   1999.9

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  • Transition of group with construction of genetic evaluation system in Kumamoto based brown-haired Japanese species.

    揖斐隆之, 守田智, 守屋和幸, 佐々木義之

    肉用牛研究会報   66 ( 66 )   8 - 9   1998.12

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  • 肉牛育種システムの構築 2. 近交係数の上昇を抑え遺伝的改良を最大にする交配システム

    揖斐隆之, 守田智, 守屋和幸, 佐々木義之

    システム農学会シンポジウム要旨集   1998   44 - 45   1998.11

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  • 肉牛育種システムの構築 1. 遺伝的評価システムの構築に伴なう集団の変遷

    佐々木義之, 揖斐隆之, 守田智, 守屋和幸

    システム農学会シンポジウム要旨集   1998   42 - 43   1998.11

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  • Elucidation of population structure of brown-haired Japanese variant group in Kumamoto prefecture based on the contribution ratio on the genetics of the typical founder bull.

    揖斐隆之, 守屋和幸, 松本道夫, 木場俊太郎, 佐々木義之

    日本畜産学会大会講演要旨   91st   175   1996.3

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  • 熊本県褐毛和種集団の集団構造の変化ならびに産地間の遺伝的関連性

    佐々木義之, 揖斐隆之, 守屋和幸, 高柳誠二, 松本道夫, 木場俊太郎

    日本畜産学会大会講演要旨   90th   144   1995.3

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Research Projects

  • 動物集団における近交退化の分子メカニズムの解明

    Grant number：20H00446  2020.04 - 2024.03

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    国枝 哲夫, 大月 純子, 佐々木 慎二, 桃沢 幸秀, 藤原 靖浩, 揖斐 隆之, 辻 岳人

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    Grant amount：\44720000 （ Direct expense: \34400000 、 Indirect expense：\10320000 ）

    野間馬の全個体について家系情報を収集し、それらに基づいて各個体の近交係数を調べた結果、野間馬集団の過去46年間の平均近交係数は0.195であり、また平均近交係数は1986年以降漸次上昇していることが明らかになり、近交化が進行していることが確認された。また、野間馬集団中の多くの個体のDNAを用いて、DNAマイクロアレイにより全ゲノムを網羅した約7万の一塩基多型（SNPs）のタイピングを行うと共に、主要組織適合性遺伝子複合体（MHC）のハプロタイプのタイピングを行った。その結果、SNPs のタイピング結果より求めた野間馬集団の平均近交係数は0.15であり、家系情報より求めた平均近交係数より若干低いもののやはり高い傾向を示した。さらにMHCクラスII領域の10遺伝子座のタイピングを行ったところ、野間馬の集団中には4ハプロタイプしか存在しないことが明らかになった。これらの結果から、当初の予想通り、野間馬の集団の近交化はかなり進行していることが確認された。また、現在飼育されている野間馬の全個体について、臨床診断、血液検査等により異常の詳細な調査も実施している。
    これまでに世界各国から収集したウマのDNAサンプルを用いて、PRDM9遺伝子の塩基配列を解析した結果、これら動物種においても他の動物種と同様に極めて多様性が高いことが確認された。またヒトにおいても日本人の集団中においてPRDM9遺伝子のZinc-Finger Domainの多様性は高く多くのハプロタイプが存在することが確認された。
    さらに、黒毛和種の集団で発生が報告された、特徴的な症状を呈して生後間もなく死亡する疾患の発症個体について、家系の調査と共に広く全ゲノムの塩基配列の解析を行ったところ、特定の遺伝子を含むゲノム広い領域における欠失が確認され、近交化にともなうゲノムの不安定化がその原因である可能性も示唆された。

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  • Genetic investigation of a hereditary disorder in Japanese Black cattle

    Grant number：16H05016  2016.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Kunieda Tetsuo

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    Grant amount：\16900000 （ Direct expense: \13000000 、 Indirect expense：\3900000 ）

    The aim of this study is to identify the causative gene for micrognathia and renal hypoplasia that occurs in Japanese Black cattle and to establish a genetic diagnostic method. The chromosomal localization of the causative gene was determined by linkage analysis using a pedigree including affected animals, and consequently a candidate gene was identified. Since the exact structure and nucleotide sequence of this gene were not clarified, we determined the nucleotide sequence of this gene. Based on these information, we investigated whether there was a causative mutation, but we could not identify the mutation that could affect the function of gene on the exons. Then, we investigated other regions of this gene including introns and finally found several nucleotide substitutions that could be specific to the affected animals

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  • Studys on the origin of domestic horse using Kazakhstan native horse

    Grant number：26660257  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    KUNIEDA TETSUO, IBI Takayuki, TOZAKI Teruaki

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    Grant amount：\4030000 （ Direct expense: \3100000 、 Indirect expense：\930000 ）

    Kazakhstan native horse has been expected to retain the genetic influences of ancestral wiled horse. In the present study, therefore, we investigated the genetic characteristics of the native horse population of Kazakhstan. We determined haplotypes of mitochondrial DNA and Y chromosome, as well as genotypes of several functional genes associated with particular traits of horse in 106 Kazakhstan native horses. The results of mitochondrial DNA haplotypes indicated that the population retains high level genetic diversity and unique genetic features in maternal lineage. On the other hand, the results of Y chromosomal haplotypes indicated relation with the common European breeds in paternal lineage. The genotypes of genes associated with gait, physical performance, and withers height suggested that the population has not been under strong selective pressure on these traits.

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  • Investigation for effects of blood coagulation factor XI deficiency on productivity to the genetic improvement of Japanese beef cattle

    Grant number：23380166  2011.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    KUNIEDA Tetsuo, TSUJI Takehito, IBI Takayuki, FUNAHASHI Hiroaki, ACOSTA Tomas

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    Grant amount：\18200000 （ Direct expense: \14000000 、 Indirect expense：\4200000 ）

    Blood coagulation factor XI deficiency is a hereditary disorder observed in Japanese Black cattle. While the clinical condition of this disorder is mild, the economical impact of this disorder is apprehended to be serious because of the remarkably high allelic frequency of the mutant allele in the population of Japanese Black cattle. We, therefore, investigated the effect of the factor XI deficiency on defects in pregnancy and delivery and carcass traits using large samples of Japanese Black cattle. As a result, we found that affected animals of factor XI deficiency have a tendencies for increased frequencies of abortion and stillborn, but no effect on the condition of calf and carcass traits. These findings will be useful to future breeding of Japanese Black cattle.

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  • 多因子疾患発現機構解明のための混合遺伝モデルによる複数QTL解析法の開発

    Grant number：13204044  2001

    日本学術振興会  科学研究費助成事業 特定領域研究(C)  特定領域研究(C)

    佐々木 義之, 揖斐 隆之

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    Grant amount：\5000000 （ Direct expense: \5000000 ）

    1)シミュレーション実験の中で、F2の個体数、QTL分散の大きさおよびQTLの組み合わせを変化させてデータを発生し、解析を行った。その結果、F2個体が1,000頭の場合、主効果に関しては2〜3%以上、エピスタシス効果については、4%以上の寄与のある効果の検出が可能であり、F2個体が200頭の場合でも、主効果に関して10%以上、エピスタシス効果に関して15〜20%以上の寄与の効果を検出できることが分かった。また、近交系間F2ラットのデータを分析した結果、インターバルマッピング法で検出されなかったQTLおよびQTL間のエピスタシスが検出され、MC-IM法の有効性が示された。
    2)ポリジーン効果ならびに環境効果を取り上げた混合遺伝モデルによる多因子疾患発現機構解明のための新たなQTL解析法の開発に対して、Metropolis-Hastings(MH)アルゴリズムおよびリバーシブルジャンプマルコフ連鎖モンテカルロ(RJMCMC)を用いたベイズ推定法に基づく手法を開発した。この方法により、想定したQTLの数、また各々のもつ効果の大きさ、染色体上の位置がいずれも偏りなく推定可能であることがシミュレーション実験によって示された。また、ポリジーン分散および環境分散についてもほぼ真値通りの結果が得られた。

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  • 熊本県褐毛和種における育種計画に関する研究

    Grant number：97J03331  1998 - 1999

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    揖斐 隆之

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    Grant amount：\1800000 （ Direct expense: \1800000 ）

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  • Course Seminar 4 （2024academic year） 3rd and 4th semester  - その他

  • Applied Animal Breeding （2024academic year） Late  - その他

  • Animal Breeding and Genetics 1 （2024academic year） Third semester  - 月5,月6

  • Animal Breeding and Genetics 2 （2024academic year） Fourth semester  - 月5,月6

  • Advanced Animal Genetics and Breeding （2024academic year） Late  - 月5～8

  • Seminar in Animal Breeding Genetics （2024academic year） Prophase  - その他

  • Seminar in Animal Breeding Genetics （2024academic year） Late  - その他

  • Seminar in Animal Breeding Genetics （2024academic year） Prophase  - その他

  • Seminar in Animal Breeding Genetics （2024academic year） Prophase  - その他

  • Undergraduate's-level thesis research （2024academic year） 1st-4th semester  - その他

  • Fundamental Generics 1 （2024academic year） Third semester  - 金3,金4

  • Fundamental Generics 1 （2024academic year） Third semester  - 金3～4

  • Fundamental Generics 2 （2024academic year） Fourth semester  - 金3,金4

  • Fundamental Generics 2 （2024academic year） Fourth semester  - 金3～4

  • Applied Biological Data Science 1 （2024academic year） Third semester  - 火1,火2

  • Applied Biological Data Science 2 （2024academic year） Fourth semester  - 火1,火2

  • Advanced Study （2024academic year） Other  - その他

  • Conservation of Bio resources （2024academic year） Third semester  - 金1～2

  • Seminars in Special Field of Study 1 （2024academic year） 1st and 2nd semester  - その他

  • Seminars in Special Field of Study 2 （2024academic year） 3rd and 4th semester  - その他

  • Course Seminar 3 （2023academic year） 1st and 2nd semester  - その他

  • Course Seminar 4 （2023academic year） 3rd and 4th semester  - その他

  • Applied　Animal Breeding （2023academic year） Late  - その他

  • Applied Animal Breeding （2023academic year） Late  - その他

  • Animal Breeding and Genetics 1 （2023academic year） Third semester  - 木5,木6

  • Animal Breeding and Genetics 2 （2023academic year） Fourth semester  - 木5,木6

  • Advanced Animal Genetics and Breeding （2023academic year） Late  - 月5,月6,月7,月8

  • Advanced Animal Genetics and Breeding （2023academic year） Late  - 月5～8

  • Seminar in Animal Breeding Genetics （2023academic year） Prophase  - その他

  • Seminar in Animal Breeding and Genetics （2023academic year） Late  - その他

  • Seminar in Animal Breeding Genetics （2023academic year） Late  - その他

  • Seminar in Animal Breeding Genetics （2023academic year） Late  - その他

  • Seminar in Animal Breeding Genetics （2023academic year） Late  - その他

  • Seminar in Animal Breeding and Genetics （2023academic year） Prophase  - その他

  • Seminar in Animal Breeding Genetics （2023academic year） Prophase  - その他

  • Seminar in Animal Breeding Genetics （2023academic year） Prophase  - その他

  • Seminar in Animal Breeding Genetics （2023academic year） Prophase  - その他

  • Undergraduate's-level thesis research （2023academic year） 1st-4th semester  - その他

  • Fundamental Generics 1 （2023academic year） Third semester  - 金3,金4

  • Fundamental Generics 1 （2023academic year） Third semester  - 金3～4

  • Fundamental Generics 2 （2023academic year） Fourth semester  - 金3,金4

  • Fundamental Generics 2 （2023academic year） Fourth semester  - 金3～4

  • Applied Biological Data Science 1 （2023academic year） Third semester  - 火1,火2

  • Applied Biological Data Science 2 （2023academic year） Fourth semester  - 火1,火2

  • Advanced Study （2023academic year） Other  - その他

  • Specific Research of Science for Bio-Production （2023academic year） Other  - その他

  • Specific Research of Science for Bio-Production （2023academic year） Year-round  - その他

  • Specific Research of Science for Bio-Production （2023academic year） Year-round  - その他

  • Conservation of Bio resources （2023academic year） Third semester  - 金1～2

  • Seminars in Special Field of Study 1 （2023academic year） 1st and 2nd semester  - その他

  • Seminars in Special Field of Study 2 （2023academic year） 3rd and 4th semester  - その他

  • Course Seminar 1 （2022academic year） 1st and 2nd semester  - 水3

  • Course Seminar 2 （2022academic year） 3rd and 4th semester  - 水3

  • Course Seminar 4 （2022academic year） 3rd and 4th semester  - その他

  • Applied　Animal Breeding （2022academic year） Late  - その他

  • Animal Breeding and Genetics 1 （2022academic year） Third semester  - 木5,木6

  • Animal Breeding and Genetics 2 （2022academic year） Fourth semester  - 木5,木6

  • Advanced Animal Genetics and Breeding （2022academic year） Late  - 月5,月6,月7,月8

  • Seminar in Animal Breeding Genetics （2022academic year） Prophase  - その他

  • Seminar in Animal Breeding and Genetics （2022academic year） Late  - その他

  • Seminar in Animal Breeding Genetics （2022academic year） Late  - その他

  • Seminar in Animal Breeding and Genetics （2022academic year） Prophase  - その他

  • Undergraduate's-level thesis research （2022academic year） 1st-4th semester  - その他

  • Fundamental Generics 1 （2022academic year） Third semester  - 金3～4

  • Fundamental Generics 1 （2022academic year） Third semester  - 金3,金4

  • Fundamental Generics 2 （2022academic year） Fourth semester  - 金3,金4

  • Fundamental Generics 2 （2022academic year） Fourth semester  - 金3～4

  • Applied Biological Data Science 1 （2022academic year） Third semester  - 火1,火2

  • Applied Biological Data Science 2 （2022academic year） Fourth semester  - 火1,火2

  • Specific Research of Science for Bio-Production （2022academic year） Year-round  - その他

  • Specific Research of Science for Bio-Production （2022academic year） Other  - その他

  • Conservation of Bio resources （2022academic year） Third semester  - 金1～2

  • Seminars in Special Field of Study 1 （2022academic year） 1st and 2nd semester  - その他

  • Seminars in Special Field of Study 2 （2022academic year） 3rd and 4th semester  - その他

  • Course Seminar 4 （2021academic year） 3rd and 4th semester  - その他

  • Animal Breeding and Genetics 1 （2021academic year） Third semester  - 木5,木6

  • Animal Breeding and Genetics 2 （2021academic year） Fourth semester  - 木5,木6

  • Advanced Animal Genetics and Breeding （2021academic year） Late  - 月5,月6,月7,月8

  • Seminar in Animal Breeding Genetics （2021academic year） Prophase  - その他

  • Seminar in Animal Breeding and Genetics （2021academic year） Late  - その他

  • Seminar in Animal Breeding Genetics （2021academic year） Late  - その他

  • Seminar in Animal Breeding and Genetics （2021academic year） Prophase  - その他

  • Undergraduate's-level thesis research （2021academic year） 1st-4th semester  - その他

  • Fundamental Generics 1 （2021academic year） Third semester  - 金3,金4

  • Fundamental Generics 1 （2021academic year） Third semester  - 金3～4

  • Fundamental Generics 2 （2021academic year） Fourth semester  - 金3,金4

  • Fundamental Generics 2 （2021academic year） Fourth semester  - 金3～4

  • Applied Biological Data Science 1 （2021academic year） Third semester  - 火1,火2

  • Applied Biological Data Science 2 （2021academic year） Fourth semester  - 火1,火2

  • Specific Research of Science for Bio-Production （2021academic year） Year-round  - その他

  • Conservation of Bio resources （2021academic year） Third semester  - 金1～2

  • Seminars in Special Field of Study 1 （2021academic year） 1st and 2nd semester  - その他

  • Seminars in Special Field of Study 2 （2021academic year） 3rd and 4th semester  - その他

  • Course Seminar 4 （2020academic year） 3rd and 4th semester  - その他

  • Applied　Animal Breeding （2020academic year） Late  - その他

  • Animal Breeding and Genetics 1 （2020academic year） Third semester  - 木5,木6

  • Animal Breeding and Genetics 2 （2020academic year） Fourth semester  - 木5,木6

  • Advanced Animal Genetics and Breeding （2020academic year） Late  - 月5,月6,月7,月8

  • Seminar in Animal Breeding Genetics （2020academic year） Prophase  - その他

  • Seminar in Animal Breeding and Genetics （2020academic year） Late  - その他

  • Seminar in Animal Breeding Genetics （2020academic year） Late  - その他

  • Seminar in Animal Breeding and Genetics （2020academic year） Prophase  - その他

  • Undergraduate's-level thesis research （2020academic year） 1st-4th semester  - その他

  • Fundamental Generics 1 （2020academic year） Third semester  - 金3,金4

  • Fundamental Generics 1 （2020academic year） Third semester  - 金3,金4

  • Fundamental Generics 2 （2020academic year） Fourth semester  - 金3,金4

  • Fundamental Generics 2 （2020academic year） Fourth semester  - 金3,金4

  • Advanced Animal Breeding and Genetics （2020academic year） Late  - 火3,火4

  • 応用生物データサイエンス学１ （2020academic year） 第３学期  - 火1,火2

  • 応用生物データサイエンス学２ （2020academic year） 第４学期  - 火1,火2

  • Specific Research of Science for Bio-Production （2020academic year） Year-round  - その他

  • Conservation of Bio resources （2020academic year） Third semester  - 金1,金2

  • Seminars in Special Field of Study 1 （2020academic year） 1st and 2nd semester  - その他

  • Seminars in Special Field of Study 2 （2020academic year） 3rd and 4th semester  - その他

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