Updated on 2024/12/12

写真a

 
IBI Takayuki
 
Organization
Faculty of Environmental, Life, Natural Science and Technology Associate Professor
Position
Associate Professor
External link

Degree

  • Agriculture ( 2002.3   Kyoto University )

  • 博士(農学) ( 2002.3   京都大学 )

Research Interests

  • Quantitative genetics

  • Population genetics

  • Animal breeding

Research Areas

  • Life Science / Animal production science  / animal breeding and genetics

Education

  • Kyoto University   大学院農学研究科博士後期課程  

    1997.4 - 2001.3

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  • Kyoto University   大学院農学研究科博士前期課程  

    1995.4 - 1997.3

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  • Kyoto University   農学部   畜産学科

    1991.4 - 1995.3

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Research History

  • Okayama University   The Graduate School of Environmental and Life Science   Associate Professor

    2012.4

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  • Okayama University   The Graduate School of Natural Science and Technology   Associate Professor

    2010.7 - 2012.3

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  • 京都大学大学院   助手

    2001.4 - 2003.3

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Professional Memberships

Committee Memberships

  • 肉用牛研究会   評議員  

    2024.4   

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    Committee type:Academic society

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  • 第72回関西畜産学会   実行委員  

    2022.1 - 2022.12   

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  • 関西畜産学会   会計  

    2017.4 - 2019.3   

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  • 日本動物遺伝育種学会   会計  

    2010.7 - 2012.9   

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  • 日本動物遺伝育種学会   会計  

    2001.4 - 2002.3   

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Papers

  • Association of a non-synonymous variant of MLH3 gene involved in meiotic recombination with conception rate of Japanese Black cattle

    Thu Nu Anh Le, Trung Ba Nguyen, Takehito Tsuji, Takayuki Ibi, Shinji Sasaki, Tetsuo Kunieda

    Animal Production Science   64 ( 17 )   2024.11

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    Publishing type:Research paper (scientific journal)   Publisher:CSIRO Publishing  

    Context Conception rate, which is an important parameter to evaluate female fertility, has been gradually decreasing in Japanese Black cattle during the past decades. Meiosis is an essential biological process in gamete formation and meiotic failure could be a cause of infertility or reduced fertility in mammals. Of note, single nucleotide polymorphisms (SNPs) in the MLH3 gene involved in meiotic recombination were reported to affect the genome-wide recombination rate of meiosis in cattle. Aims The present study was conducted to investigate the association of SNPs in the MLH3 gene with conception rate in Japanese Black cattle. Methods On the basis of the reproductive data of 2045 Japanese Black cattle born from 1990 to 2009, we selected two groups of the reproductive females with high conception rate (n = 103) and low conception rate (n = 109). Then, we genotyped the SNPs in MLH3 gene in both reproductive groups by sequencing and polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). The significant association of SNPs with conception rate in Japanese Black cattle was estimated by Fisher’s exact test, by using R commander. Key results We found the presence of five SNPs of MLH3 gene in Japanese Black cattle, including non-synonymous variants of MLH3 N408S that has been reported to be involved in meiotic recombination rate and MLH3 S591G newly identified in the present study. Comparison of genotype distributions and allele frequencies of the MLH3 N408S between high and low conception-rate groups indicated a significant difference between these groups, suggesting that this SNP is associated with conception rate. However, there is no significant difference between MLH3 S591G with conception rate in Japanese Black cattle. Conclusions We found a significant association between a non-synonymous variant of MLH3 N408S and conception rate, suggesting that this SNP can be a potential marker for selecting the Japanese Black cattle for improving fertility. We also identified a novel non-synonymous variant of MLH3 S591G in Japanese Black cattle. Implications These findings will be informative for future marker-assisted selection to improve the fertility of Japanese Black cattle. This is the first report of a genetic variant implicated in meiotic recombination being linked to female fertility in cattle.

    DOI: 10.1071/an23418

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  • A genome-wide association study reveals a quantitative trait locus for calf mortality on chromosome 9 in Japanese Black cattle Reviewed

    S. Sasaki, T. Ibi

    ANIMAL GENETICS   52 ( 2 )   214 - 216   2021.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    Calf mortality is a major problem affecting cattle production. To identify genetic variants associated with calf mortality in Japanese Black cattle, we evaluated calf mortality as a categorical trait using a threshold model and conducted a GWAS. We identified two SNPs between 32 549 297 and 32 606 924 bp on bovine chromosome 9 that were significantly associated with calf mortality from 61 to 180 days after birth. The SNP showing the highest association was localized at a region 624 bp downstream of exon 4 of the anti-silencing function 1A histone chaperone gene (ASF1A) that promotes DNA damage repair, and the null mice, which exhibit pre- and postnatal lethality. This association was also detected using the breeding value of 334 sires. The frequency of the risk allele in Japanese Black cattle from locations across Japan was 0.013; although the frequency of ASF1A risk allele was low, it is widespread in the Japanese Black cattle population. Thus, it may be necessary to routinely monitor the cattle population for the presence of this allele.

    DOI: 10.1111/age.13048

    Web of Science

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  • Identification of deleterious recessive haplotypes and candidate deleterious recessive mutations in Japanese Black cattle Reviewed

    Shinji Sasaki, Toshio Watanabe, Takayuki Ibi, Kiyotoshi Hasegawa, Yoichi Sakamoto, Shunsuke Moriwaki, Kazuhito Kurogi, Atsushi Ogino, Takanori Yasumori, Hiroyuki Wakaguri, Eiji Muraki, Youko Miki, Yuichi Yoshida, Yoshinobu Inoue, Ichiro Tabuchi, Ken Iwao, Taichi Arishima, Keisuke Kawashima, Manabu Watanabe, Sumio Sugano, Yoshikazu Sugimoto, Yutaka Suzuki

    SCIENTIFIC REPORTS   11 ( 1 )   2021.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE RESEARCH  

    Intensive use of a few elite sires has increased the risk of the manifestation of deleterious recessive traits in cattle. Substantial genotyping data gathered using single-nucleotide polymorphism (SNP) arrays have identified the haplotypes with homozygous deficiency, which may compromise survival. We developed Japanese Black cattle haplotypes (JBHs) using SNP array data (4843 individuals) and identified deleterious recessive haplotypes using exome sequencing of 517 sires. We identified seven JBHs with homozygous deficiency. JBH_10 and JBH_17 were associated with the resuming of estrus after artificial insemination, indicating that these haplotypes carried deleterious mutations affecting embryonic survival. The exome data of 517 Japanese Black sires revealed that AC_000165.1:g.85341291C>G of IARS in JBH_8_2, AC_000174.1:g.74743512G>T of CDC45 in JBH_17, and a copy variation region (CNVR_27) of CLDN16 in JBH_1_1 and JBH_1_2 were the candidate mutations. A novel variant AC_000174.1:g.74743512G>T of CDC45 in JBH_17 was located in a splicing donor site at a distance of 5 bp, affecting pre-mRNA splicing. Mating between heterozygotes of JBH_17 indicated that homozygotes carrying the risk allele died around the blastocyst stage. Analysis of frequency of the CDC45 risk allele revealed that its carriers were widespread throughout the tested Japanese Black cattle population. Our approach can effectively manage the inheritance of recessive risk alleles in a breeding population.

    DOI: 10.1038/s41598-021-86225-y

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  • A 44-kb deleted-type copy number variation is associated with decreasing complement component activity and calf mortality in Japanese Black cattle Reviewed

    Shinji Sasaki, Youko Miki, Takayuki Ibi, Hiroyuki Wakaguri, Yuichi Yoshida, Yoshikazu Sugimoto, Yutaka Suzuki

    BMC GENOMICS   22 ( 1 )   2021.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BMC  

    Background Calf mortality generally occurs in calves prior to weaning, which is a serious problem in cattle breeding. Several causative variants of monogenic Mendelian disorders in calf mortality have been identified, whereas genetic factors affecting the susceptibility of calves to death are not well known. To identify variants associated with calf mortality in Japanese Black cattle, we evaluated calf mortality as a categorical trait with a threshold model and performed a genome-wide copy number variation (CNV) association study on calf mortality. Results We identified a 44-kb deleted-type CNV ranging from 103,317,687 to 103,361,802 bp on chromosome 5, which was associated with the mortality of 1-180-day-old calves. The CNV harbored C1RL, a pseudogene, and an IncRNA localized in the C1R and C1S gene cluster, which is a component of the classical complement activation pathway for immune complexes for infectious pathogens. The average complement activity in CNVR_221 homozygotes at postnatal day 7 was significantly lower than that of wild-type animals and heterozygotes. The frequency of the risk allele in dead calves suffering from diarrhea and pneumonia and in healthy cows was 0.35 and 0.28, respectively (odds ratio = 2.2, P = 0.016), suggesting that CNVR_221 was associated with the mortality of Japanese Black calves suffering from an infectious disease. Conclusions This study identified a deleted-type CNV associated with the mortality of 1-180-day-old calves. The complement activity in CNVR_221 homozygotes was significantly lower than that in heterozygotes and wild type animals. The frequency of the risk allele was higher in dead calves suffering from an infectious disease than in healthy cows. These results suggest that the existence of CNVR_221 in calves could be attributed to a reduction in complement activity, which in turn leads to susceptibility to infections. Thus, the risk allele could serve as a useful marker to reduce the mortality of infected Japanese Black calves.

    DOI: 10.1186/s12864-021-07415-6

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  • Genetic variants of RNF212 involved in meiotic recombination rate and its relation with conception rate in Japanese Black cattle Reviewed

    Thu Nu Anh LE, Trung Ba NGUYEN, Ripon Chandra PAUL, Yu OKUDA, Takehito TSUJI, Takayuki IBI, Shinji SASAKI, Tetsuo KUNIEDA

    The Journal of Animal Genetics   49 ( 1 )   13 - 17   2021

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    Publishing type:Research paper (scientific journal)   Publisher:Japanese Society of Animal Breeding and Genetics  

    DOI: 10.5924/abgri.49.13

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Books

  • 動物遺伝育種学

    祝前, 博明, 国枝, 哲夫, 野村, 哲郎, 万年, 英之, 西堀, 正英, 下桐, 猛, 揖斐, 隆之, 石川, 明, 大山, 憲二, 三宅, 武, 古川, 力, 長嶺, 慶隆, 山田, 宜永, 広岡, 博之

    朝倉書店  2017.3  ( ISBN:9784254450309

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    Total pages:vi, 204p   Language:Japanese

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MISC

  • 岡山県固有の黒毛和種希少系統の遺伝学的特徴の解析

    米田一裕, 奥田ゆう, Siqintuya, 西牧孝洋, 松本大和, 宮崎義之, 揖斐隆之, 辻岳人, 国枝哲夫

    日本畜産学会報   87 ( 1 )   1‐10(J‐STAGE) - 10   2016.2

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    Language:Japanese   Publisher:公益社団法人 日本畜産学会  

    The effective population size of Japanese Black cattle has been significantly reduced and maintaining the genetic diversity of the population is important for breeding of Japanese Black cattle. For the conservation of genetic diversity, use of different lines of the breed with unique genetic characteristics for breeding, instead of intensive use of sires of few particular lines as common in current breeding of Japanese Black cattle, will be effective to prevent the genetic homogenization of the population. In the present study, we performed genetic characterization a population of a rare line of Japanese Black cattle, which has been originated from ancestral "Tsuru-ushi" in Okayama prefecture. By using microsatellite markers, allelic richness, and average observed and expected heterozygosity are 3.48, 0.514, and 0.511, respectively, and these values were lower than those of most of the Japanese Black cattle local subpopulations compared. The result of the clustering analyses indicated that the animals of the rare line formed a single group with a cluster that was clearly distinguished from the other populations. Sequence analysis of mitochondrial D-loop region revealed that only two haplotypes were observed in the population and 80% of the animals in the population possess a single haplotype. However, the other haplotype was a novel unique haplotype that has not been reported in cattle. Genotyping of six genes associated with important traits revealed that <i>SREBP1</i> and <i>NCAPG</i> loci were fixed for a single allele in the population and more than 90% of animals possess an allele of <i>MC1R</i> locus. These findings indicated that while genetic diversity of the population of the rare line is lower than those of the Japanese Black cattle local subpopulations, this population has unique genetic characteristics that were distinguished from the other populations and, therefore, the rare line is important for maintaining genetic diversity of Japanese Black cattle.

    DOI: 10.2508/chikusan.87.1

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  • Survey on the undernourished university students who tend to lack breakfast; a proposal for a novel viewpoint for the improvement

    田村隆, 揖斐隆之, 稲垣賢二, 久保康隆, 奥田潔

    岡山大学農学部学術報告(Web)   105 ( 105 )   1-5 (WEB ONLY) - 5   2016.2

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    Language:Japanese   Publisher:岡山大学農学部  

    This study investigated the current status and causes underneath the life of university students who tend to lack breakfast at a relatively high frequency, and statistical analysis on consequences leading to such lack of well-nourished eating habitat in their university life. In October 2014, self-assessed questionnaires were administered to over 150 faculty students. It contained questions about breakfast habits, time allowance for the morning class, and lunchtime setting in their high school timetable. Breakfast states were clearly separated in three groups : 68% of students regularly have breakfast throughout the weekdays, 21% students skipping the breakfast occasionally, and 11% student no habit for breakfast at all. The survey on the high school lives revealed that 70% students used to have lunch 30 min later than the lunchtime set in the university timetable, 7% of them had the lunch time even more than 1 h later. Lunchtime varies among high schools, and statistical significance was revealed (p<0.01) that schools with higher deviation scores tend have late lunch beyond 12: 30. Accordingly, university students were given directions to prepare for the timetable reform on postulation of having lunch time over one o'clock. After continuous survey on the breakfast habits during the second semester, more than 90% of students established the habit of breakfast regularly in their university lives with the improved consciousness toward well-balanced healthy breakfast contents for their higher level of education quality.

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  • 「豊味(うま)いの証」豊後牛肉生産技術の確立―牛肉中のオレイン酸含有率を取り入れた産肉能力育種価手法の検討―

    志村英明, 利光昭彦, 衞藤央好, 安高康幸, 揖斐隆之

    九州農業研究発表会専門部会発表要旨集   78th   71   2015.8

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    Language:Japanese  

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  • 岡山県のジャージー集団におけるBCO2遺伝子の新たな変異と脂肪淡黄色との関連性

    清水佑起, 石倉健一, 揖斐隆之, 国枝哲夫, 辻岳人

    日本畜産学会大会講演要旨   119th   115   2015.3

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    Language:Japanese  

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  • オレイン酸含有率を用いた新たな育種価評価の確立

    利光昭彦, 手島久智, 衛藤央好, 三輪友樹, 藤田達男, 揖斐隆之

    九州沖縄農業試験研究の成果情報(Web)   2015   WEB ONLY   2015

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    Language:Japanese  

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Research Projects

  • 動物集団における近交退化の分子メカニズムの解明

    Grant number:20H00446  2020.04 - 2024.03

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    国枝 哲夫, 大月 純子, 佐々木 慎二, 桃沢 幸秀, 藤原 靖浩, 揖斐 隆之, 辻 岳人

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    Grant amount:\44720000 ( Direct expense: \34400000 、 Indirect expense:\10320000 )

    野間馬の全個体について家系情報を収集し、それらに基づいて各個体の近交係数を調べた結果、野間馬集団の過去46年間の平均近交係数は0.195であり、また平均近交係数は1986年以降漸次上昇していることが明らかになり、近交化が進行していることが確認された。また、野間馬集団中の多くの個体のDNAを用いて、DNAマイクロアレイにより全ゲノムを網羅した約7万の一塩基多型(SNPs)のタイピングを行うと共に、主要組織適合性遺伝子複合体(MHC)のハプロタイプのタイピングを行った。その結果、SNPs のタイピング結果より求めた野間馬集団の平均近交係数は0.15であり、家系情報より求めた平均近交係数より若干低いもののやはり高い傾向を示した。さらにMHCクラスII領域の10遺伝子座のタイピングを行ったところ、野間馬の集団中には4ハプロタイプしか存在しないことが明らかになった。これらの結果から、当初の予想通り、野間馬の集団の近交化はかなり進行していることが確認された。また、現在飼育されている野間馬の全個体について、臨床診断、血液検査等により異常の詳細な調査も実施している。
    これまでに世界各国から収集したウマのDNAサンプルを用いて、PRDM9遺伝子の塩基配列を解析した結果、これら動物種においても他の動物種と同様に極めて多様性が高いことが確認された。またヒトにおいても日本人の集団中においてPRDM9遺伝子のZinc-Finger Domainの多様性は高く多くのハプロタイプが存在することが確認された。
    さらに、黒毛和種の集団で発生が報告された、特徴的な症状を呈して生後間もなく死亡する疾患の発症個体について、家系の調査と共に広く全ゲノムの塩基配列の解析を行ったところ、特定の遺伝子を含むゲノム広い領域における欠失が確認され、近交化にともなうゲノムの不安定化がその原因である可能性も示唆された。

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  • Genetic investigation of a hereditary disorder in Japanese Black cattle

    Grant number:16H05016  2016.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Kunieda Tetsuo

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    Grant amount:\16900000 ( Direct expense: \13000000 、 Indirect expense:\3900000 )

    The aim of this study is to identify the causative gene for micrognathia and renal hypoplasia that occurs in Japanese Black cattle and to establish a genetic diagnostic method. The chromosomal localization of the causative gene was determined by linkage analysis using a pedigree including affected animals, and consequently a candidate gene was identified. Since the exact structure and nucleotide sequence of this gene were not clarified, we determined the nucleotide sequence of this gene. Based on these information, we investigated whether there was a causative mutation, but we could not identify the mutation that could affect the function of gene on the exons. Then, we investigated other regions of this gene including introns and finally found several nucleotide substitutions that could be specific to the affected animals

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  • Studys on the origin of domestic horse using Kazakhstan native horse

    Grant number:26660257  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    KUNIEDA TETSUO, IBI Takayuki, TOZAKI Teruaki

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    Grant amount:\4030000 ( Direct expense: \3100000 、 Indirect expense:\930000 )

    Kazakhstan native horse has been expected to retain the genetic influences of ancestral wiled horse. In the present study, therefore, we investigated the genetic characteristics of the native horse population of Kazakhstan. We determined haplotypes of mitochondrial DNA and Y chromosome, as well as genotypes of several functional genes associated with particular traits of horse in 106 Kazakhstan native horses. The results of mitochondrial DNA haplotypes indicated that the population retains high level genetic diversity and unique genetic features in maternal lineage. On the other hand, the results of Y chromosomal haplotypes indicated relation with the common European breeds in paternal lineage. The genotypes of genes associated with gait, physical performance, and withers height suggested that the population has not been under strong selective pressure on these traits.

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  • Investigation for effects of blood coagulation factor XI deficiency on productivity to the genetic improvement of Japanese beef cattle

    Grant number:23380166  2011.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    KUNIEDA Tetsuo, TSUJI Takehito, IBI Takayuki, FUNAHASHI Hiroaki, ACOSTA Tomas

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    Grant amount:\18200000 ( Direct expense: \14000000 、 Indirect expense:\4200000 )

    Blood coagulation factor XI deficiency is a hereditary disorder observed in Japanese Black cattle. While the clinical condition of this disorder is mild, the economical impact of this disorder is apprehended to be serious because of the remarkably high allelic frequency of the mutant allele in the population of Japanese Black cattle. We, therefore, investigated the effect of the factor XI deficiency on defects in pregnancy and delivery and carcass traits using large samples of Japanese Black cattle. As a result, we found that affected animals of factor XI deficiency have a tendencies for increased frequencies of abortion and stillborn, but no effect on the condition of calf and carcass traits. These findings will be useful to future breeding of Japanese Black cattle.

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  • 多因子疾患発現機構解明のための混合遺伝モデルによる複数QTL解析法の開発

    Grant number:13204044  2001

    日本学術振興会  科学研究費助成事業 特定領域研究(C)  特定領域研究(C)

    佐々木 義之, 揖斐 隆之

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    Grant amount:\5000000 ( Direct expense: \5000000 )

    1)シミュレーション実験の中で、F2の個体数、QTL分散の大きさおよびQTLの組み合わせを変化させてデータを発生し、解析を行った。その結果、F2個体が1,000頭の場合、主効果に関しては2〜3%以上、エピスタシス効果については、4%以上の寄与のある効果の検出が可能であり、F2個体が200頭の場合でも、主効果に関して10%以上、エピスタシス効果に関して15〜20%以上の寄与の効果を検出できることが分かった。また、近交系間F2ラットのデータを分析した結果、インターバルマッピング法で検出されなかったQTLおよびQTL間のエピスタシスが検出され、MC-IM法の有効性が示された。
    2)ポリジーン効果ならびに環境効果を取り上げた混合遺伝モデルによる多因子疾患発現機構解明のための新たなQTL解析法の開発に対して、Metropolis-Hastings(MH)アルゴリズムおよびリバーシブルジャンプマルコフ連鎖モンテカルロ(RJMCMC)を用いたベイズ推定法に基づく手法を開発した。この方法により、想定したQTLの数、また各々のもつ効果の大きさ、染色体上の位置がいずれも偏りなく推定可能であることがシミュレーション実験によって示された。また、ポリジーン分散および環境分散についてもほぼ真値通りの結果が得られた。

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Class subject in charge

  • Course Seminar 4 (2024academic year) 3rd and 4th semester  - その他

  • Applied Animal Breeding (2024academic year) Late  - その他

  • Animal Breeding and Genetics 1 (2024academic year) Third semester  - 月5,月6

  • Animal Breeding and Genetics 2 (2024academic year) Fourth semester  - 月5,月6

  • Advanced Animal Genetics and Breeding (2024academic year) Late  - 月5~8

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