Faculty Profiles - USHIJIMA Koichiro

MISC - USHIJIMA Koichiro

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Phenotype analysis of transgenic tomato fruit with suppressed EIN3/EIL

HARANO T., CHAMITA D., YUCATAN N., NAKANO R., USHIJIMA K., INDABA A., KUBO Y.

6 ( 2 ) 649 - 649 2007.9

Characterization of cell wall proteins related with fruit softening in European pear

BANDO M., USHIJIMA K., NAKANO R., INABA A., KUBO Y.

6 ( 1 ) 289 - 289 2007.3

β-Galactosidase and α-L-Arabinofuranosidase Activities and Gene Expression in European and Chinese Pear Fruit During Ripening :

Mwaniki Mercy W., Mathooko Francis M., Hiwasa Kyoko, Tateishi Akira, Yokotani Naoki, Ushijima Koichiro, Nakano Ryohei, Inaha Akitsugu, Kubo Yasutaka

Journal of the Japanese Society for Horticultural Science 76 ( 1 ) 85 - 90 2007

Language：English Publisher：Japanese Society for Horticultural Science

Substantial decreases in cell wall bound galactosyl and arabinosyl residues are two of the most evident cell wall compositional changes that occur during fruit ripening. The roles of β-galactosidase (β-Gal) and α-L-arabinofuranosidase (α-Af), the enzymes responsible for these respective losses, were investigated and compared in European (Pyrus communis L. 'La France') and Chinese (Pyrus bretschneideri Rehd. 'Yali') pear fruits which exhibit different softening characteristics during ripening. The increase in the activities of β-Gal and α-Af during ripening in both types of pear fruit correlated well with an increase in climacteric ethylene production, and a concomitant decrease in flesh firmness in 'La France' fruit. However, there was no noticeable decrease in 'Yali' flesh firmness even after 28 days of storage at room temperature. In both fruit types, enzyme activity and the accumulation of transcripts hybridizing with PpGAL1, PpGAL4, PpARF2, and PcARF1 increased with fruit ripening. Increases in gene expression and enzyme activities in 'Yali' fruit with no detectable softening during ripening indicate that β-Gal and α-Af may not mediate difference in fruit softening between two pears, but that they could play some role(s) in cell wall changes, perhaps in cooperation with other cell wall-modifying enzymes such as polygalacturonase.

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Other Link： http://dl.ndl.go.jp/info:ndljp/pid/10471864

Comparative characterization of fruit development in 'Nakataniwase', 'Tonewase' and 'Hiratanenashi' Japanese persimmons and inhibition of postharvest fruit softening

SAGARA H., HARIMA S., NAKANO R., USHIJIMA K., KUBO Y., INABA A.

75 ( 2 ) 409 - 409 2006.9

The responsibility of ripening-related characteristics to ethylene in 'Honey Dew' melon

NISHIYAMA K., USHIJIMA K., KATO K., NAKANO R., INABA A., KUBO Y.

75 ( 2 ) 610 - 610 2006.9

メロン品種における果実成熟特性とエチレン関連遺伝子の発現

西山精視, 牛島幸一郎, 加藤鎌司, 稲葉昭次, 久保康隆

園芸学会雑誌別冊 75 ( 1 ) 2006

Comparison of fruit softening and expression pattern of polygalacturonase gene among four melon lines

NISHIYAMA K., MIYAZAKI T., USHIJIMA K., KATO K., INABA A., KUBO Y.

74 ( 2 ) 553 - 553 2005.10

Cloning and Expression of Ripening-related MADS Family Genes in Grape Berry

YAMANE M., OZAKI M., NAKANO R., USHIJIMA K., KUBO Y., INABA A.

74 ( 1 ) 383 - 383 2005.3

Expression of softening-related genes in melon fruit during ripening

NISHIYAMA K., MIYAZAKI T., USHIJIMA K., KATO K., INABA A., KUBO Y.

74 ( 1 ) 401 - 401 2005.3

Suppression of genetic recombination at S locus of sweet cherry

IKEDA K, USHIJIMA K, YAMANE H, IEZZONI Amy, TAO R

園芸学会雑誌. 別冊, 園芸学会大会研究発表 73 ( 2 ) 557 - 557 2004.9

Analysis of self-compatible S^f haplotype of Japanese apricot

KAKEHI E, USHIJIMA K, YAMANE H, TAO R

園芸学会雑誌. 別冊, 園芸学会大会研究発表 73 ( 2 ) 558 - 558 2004.9

β-galactosidase and arabinofuranosidase activities and gene expression in three types of pear fruit

MWANIKI M. W., MATHOOKO F. M., HIWASA K., TATEISHI A., USHIJIMA K., INABA A., KUBO Y.

73 ( 2 ) 517 - 517 2004.9

Molecular markers for the self-compatible S-4 '-haplotype, a pollen-part mutant in sweet cherry (Prunus avium L.)

K Ikeda, A Watari, K Ushijima, H Yamane, NR Hauck, AF Iezzoni, R Tao

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE 129 ( 5 ) 724 - 728 2004.9

Language：English Publisher：AMER SOC HORTICULTURAL SCIENCE

S-4' is a pollen-part mutant in sweet cherry (Prunus avium L.) that is extensively used to develop self-compatible cultivars. The S-4'-haplotype is known to have a functional stylar component and a nonfunctional pollen component. The pollen component in sweet cherry necessary for the specificity of the pollen reaction is believed to be an S-haplotype specific F-box protein gene, called SFB. This study describes two molecular markers that distinguish between SFB4 and SFB4' by taking advantage of a four base pair deletion in the mutant allele. The resulting polymerase chain reaction (PCR) products can either be separated directly on a polyacrylamide gel or they can be subjected to restriction enzyme digestion and the different sized products can be visualized on an agarose gel. The latter technique utilizes restriction sites created in the PCR products from the SFB4' allele, but not the SFB4 allele. Because the primer sets created differential restriction sites, these primer sets were termed dCAPS (derived cleaved amplified polymorphism sequence) markers. These molecular assays can be used to verify self-compatibility conferred by the S-4-haplotype.

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Primary structural features of the S haplotype-specific protein, SFB, in Prunus

IKEDA K, USHIJIMA K, YAMANE H, SASSA H, IEZZONI A. F, KOHN J. R, TAO R

園芸学会雑誌. 別冊, 園芸学会大会研究発表 73 ( 1 ) 180 - 180 2004.4