2022/07/02 更新

写真a

コンドウ ヒデキ
近藤 秀樹
KONDO Hideki
所属
資源植物科学研究所 准教授
職名
准教授
外部リンク

学位

  • 博士(農学) ( 岡山大学 )

研究キーワード

  • 植物

  • メタゲノム

  • ウイルス

  • ベクター

  • 昆虫ウイルス

  • 植物ウイルス

  • 菌類ウイルス

  • 植物病理学

  • 進化

  • RNAサイレンシング

  • 分子進化

  • ラブドウイルス

  • マイナス鎖RNAウイルス

  • ウイルス化石

研究分野

  • 環境・農学 / 植物保護科学

  • ライフサイエンス / 応用分子細胞生物学

経歴

  • - 岡山大学資源植物科学研究所 准教授

    2014年 - 現在

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  • 岡山大学資源植物科学研究所 助教   Institute of Plant Science and Resources

    2007年 - 2014年

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  • 岡山大学資源生物科学研究所 助手

    1993年 - 2006年

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所属学協会

委員歴

  • 国際ウイルス分類委員会 ICTV Totiviridae Study Group  

    2021年 - 現在   

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  • 国際ウイルス分類委員会 ICTV Mymonaviridae Study Group  

    2021年 - 現在   

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  • 日本植物病理学会   原著編集委員  

    2020年1月 - 2021年12月   

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  • 日本植物病理学会   植物ウイルス分類委員会委員  

    2018年 - 現在   

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    団体区分:学協会

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  • 国際ウイルス分類委員会 ICTV   Rhabdoviridae Study Group  

    2017年 - 現在   

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    団体区分:学協会

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  • 植物ウイルス研究会   幹事(会計)  

    2017年 - 現在   

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  • Frontiers in Microbiology/ Frontiers in Plant Science   Review Editor  

    2017年 - 現在   

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▼全件表示

 

論文

  • Mycovirus Diversity and Evolution Revealed/Inferred from Recent Studies. 国際誌

    Hideki Kondo, Leticia Botella, Nobuhiro Suzuki

    Annual review of phytopathology   2022年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    High-throughput virome analyses with various fungi, from cultured or uncultured sources, have led to the discovery of diverse viruses with unique genome structures and even neo-lifestyles. Examples in the former category include splipalmiviruses and ambiviruses. Splipalmiviruses, related to yeast narnaviruses, have multiple positive-sense (+) single-stranded (ss) RNA genomic segments that separately encode the RNA-dependent RNA polymerase motifs, the hallmark of RNA viruses (members of the kingdom Orthornavirae). Ambiviruses appear to have an undivided ssRNA genome of 3∼5 kb with two large open reading frames (ORFs) separated by intergenic regions. Another narna-like virus group has two fully overlapping ORFs on both strands of a genomic segment that span more than 90% of the genome size. New virus lifestyles exhibited by mycoviruses include the yado-kari/yado-nushi nature characterized by the partnership between the (+)ssRNA yadokarivirus and an unrelated dsRNA virus (donor of the capsid for the former) and the hadaka nature of capsidless 10-11 segmented (+)ssRNA accessible by RNase in infected mycelial homogenates. Furthermore, dsRNA polymycoviruses with phylogenetic affinity to (+)ssRNA animal caliciviruses have been shown to be infectious as dsRNA-protein complexes or deproteinized naked dsRNA. Many previous phylogenetic gaps have been filled by recently discovered fungal and other viruses, which have provided interesting evolutionary insights. Phylogenetic analyses and the discovery of natural and experimental cross-kingdom infections suggest that horizontal virus transfer may have occurred and continue to occur between fungi and other kingdoms. Expected final online publication date for the Annual Review of Phytopathology, Volume 60 is August 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

    DOI: 10.1146/annurev-phyto-021621-122122

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  • First Report of Chinese wheat mosaic virus Infecting Barley in Japan. 国際誌

    Hideki Kondo, Hidekazu Masejima, Kazuyuki Maruyama, Miki Fujita, Takehiro Ohki

    Plant disease   2022年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Chinese wheat mosaic virus (CWMV), a member of the genus Furovirus in the family Virgaviridae (Adams et al. 2017), has a positive-sense RNA genome and is transmitted by Polymyxa graminis. CWMV is a causal agent of yellow mosaic disease in winter wheat in China (Guo et al. 2019). CWMV has also been detected in wheat plants in limited areas of the northern Japan (Nagano and Iwate Prefectures) (Fuji et al. 2022; Maeshima et al. 2010; Shirako and Maejima 2008). In preliminary tests using Western blotting with an antiserum raised against CWMV capsid protein (by Dr Y. Shirako, Tokyo University), we detected a furovirus in a breeding line of barley, "Tozan Kawa 111" (Hordeum vulgare L.) (collected in April 2012), grown in an experimental field infested with CWMV and wheat yellow mosaic virus (genus Bymovirus) in Nagano Prefecture. To investigate the infection of barley plants with cereal plant-associated soil-borne viruses, we collected leaf samples of "Tozan Kawa 111" plants (ten plants) showing yellow mosaic symptoms, but with not apparent wilting or stunting (Fig. S1A) in April 2016 (2015/16 growing season). Total RNA was extracted from symptomatic leave samples with TaKaRa RNAiso reagent (TaKaRa Bio) and subjected to reverse transcription polymerase chain reaction (RT-PCR) to detect virus agents. After cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific) with random hexamers, PCR amplification with QuickTaq HS Dye Mix (Toyobo Co.) was conducted using primer sets specific to two furoviruses and three bymoviruses (Fig. S1B) known to infect wheat and/or barley plants in Japan (Tamada and Kondo 2013). RT-PCR analysis detected infection with CWMV in the leaf samples of "Tozan Kawa 111" plants (Fig. S1B), but not the other soil-borne viruses tested. The amplified PCR products (752 and 718 bp for CWMV RNA1 and RNA2, respectively) were purified by Wizard® SV Gel and PCR Clean-Up System (Promega) and subjected to Sanger sequencing to confirm their nucleotide sequences. The virus sequences from PCR amplicons were deposited in GenBank/DDBJ/ENA with accession numbers LC657081 (RNA1) and LC657082 (RNA2). Nucleotide Basic Local Alignment Search Tool (BLASTn) analysis showed that the sequences have 98.7% and 98.8% nucleotide sequence identity with RNA1 of CWMV Japanese northern isolate (accession No, AB299271) and RNA2 of CWMV Nagano-A isolate (AB935554), respectively. Identical sequences were also found in symptomatic wheat leaf samples ("Fukuho Komugi" cultivar) obtained from the same field (Fig. S1B). Rod-shaped particles were observed by transmission electron microscopy (Hitachi H-7650) in symptomatic "Tozan Kawa 111" leaf samples (Fig. S1C). Using reverse transcription loop-mediated isothermal amplification (Fukuta et al. 2013), CWMV was detected in "Tozan Kawa 111" plants in the same field in the 2015/16-2017/18 growing seasons, but not in the 2018/19-2020/21 growing seasons (Fig. S1D). In the 2015/16-2017/18 growing seasons, CWMV was detected in the barley plants (pooled five plant samples) cultivar "Kashima-mugi", which showed similar yellow mosaic symptoms (Fig. S1D), but not in most of the other barley variants planted in the field. To our knowledge, this is the first report of CWMV field infection in plants other than wheat (Kuhne 2009). Further extensive virus screening in the fields and virus inoculation experiments are necessary to understand the pathology of CWMV in barley and possibly in other cereal crops.

    DOI: 10.1094/PDIS-12-21-2803-PDN

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  • Identification of a Novel Quinvirus in the Family Betaflexiviridae That Infects Winter Wheat. 国際誌

    Hideki Kondo, Naoto Yoshida, Miki Fujita, Kazuyuki Maruyama, Kiwamu Hyodo, Hiroshi Hisano, Tetsuo Tamada, Ida Bagus Andika, Nobuhiro Suzuki

    Frontiers in microbiology   12   715545 - 715545   2021年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Yellow mosaic disease in winter wheat is usually attributed to the infection by bymoviruses or furoviruses; however, there is still limited information on whether other viral agents are also associated with this disease. To investigate the wheat viromes associated with yellow mosaic disease, we carried out de novo RNA sequencing (RNA-seq) analyses of symptomatic and asymptomatic wheat-leaf samples obtained from a field in Hokkaido, Japan, in 2018 and 2019. The analyses revealed the infection by a novel betaflexivirus, which tentatively named wheat virus Q (WVQ), together with wheat yellow mosaic virus (WYMV, a bymovirus) and northern cereal mosaic virus (a cytorhabdovirus). Basic local alignment search tool (BLAST) analyses showed that the WVQ strains (of which there are at least three) were related to the members of the genus Foveavirus in the subfamily Quinvirinae (family Betaflexiviridae). In the phylogenetic tree, they form a clade distant from that of the foveaviruses, suggesting that WVQ is a member of a novel genus in the Quinvirinae. Laboratory tests confirmed that WVQ, like WYMV, is potentially transmitted through the soil to wheat plants. WVQ was also found to infect rye plants grown in the same field. Moreover, WVQ-derived small interfering RNAs accumulated in the infected wheat plants, indicating that WVQ infection induces antiviral RNA silencing responses. Given its common coexistence with WYMV, the impact of WVQ infection on yellow mosaic disease in the field warrants detailed investigation.

    DOI: 10.3389/fmicb.2021.715545

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  • Establishment of Neurospora crassa as a model organism for fungal virology. 国際誌

    Shinji Honda, Ana Eusebio-Cope, Shuhei Miyashita, Ayumi Yokoyama, Annisa Aulia, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki

    Nature communications   11 ( 1 )   5627 - 5627   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The filamentous fungus Neurospora crassa is used as a model organism for genetics, developmental biology and molecular biology. Remarkably, it is not known to host or to be susceptible to infection with any viruses. Here, we identify diverse RNA viruses in N. crassa and other Neurospora species, and show that N. crassa supports the replication of these viruses as well as some viruses from other fungi. Several encapsidated double-stranded RNA viruses and capsid-less positive-sense single-stranded RNA viruses can be experimentally introduced into N. crassa protoplasts or spheroplasts. This allowed us to examine viral replication and RNAi-mediated antiviral responses in this organism. We show that viral infection upregulates the transcription of RNAi components, and that Dicer proteins (DCL-1, DCL-2) and an Argonaute (QDE-2) participate in suppression of viral replication. Our study thus establishes N. crassa as a model system for the study of host-virus interactions.

    DOI: 10.1038/s41467-020-19355-y

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  • Facilitative and synergistic interactions between fungal and plant viruses. 査読 国際誌

    Ruiling Bian, Ida Bagus Andika, Tianxing Pang, Ziqian Lian, Shuang Wei, Erbo Niu, Yunfeng Wu, Hideki Kondo, Xili Liu, Liying Sun

    Proceedings of the National Academy of Sciences of the United States of America   117 ( 7 )   3779 - 3788   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Plants and fungi are closely associated through parasitic or symbiotic relationships in which bidirectional exchanges of cellular contents occur. Recently, a plant virus was shown to be transmitted from a plant to a fungus, but it is unknown whether fungal viruses can also cross host barriers and spread to plants. In this study, we investigated the infectivity of Cryphonectria hypovirus 1 (CHV1, family Hypoviridae), a capsidless, positive-sense (+), single-stranded RNA (ssRNA) fungal virus in a model plant, Nicotiana tabacum CHV1 replicated in mechanically inoculated leaves but did not spread systemically, but coinoculation with an unrelated plant (+)ssRNA virus, tobacco mosaic virus (TMV, family Virgaviridae), or other plant RNA viruses, enabled CHV1 to systemically infect the plant. Likewise, CHV1 systemically infected transgenic plants expressing the TMV movement protein, and coinfection with TMV further enhanced CHV1 accumulation in these plants. Conversely, CHV1 infection increased TMV accumulation when TMV was introduced into a plant pathogenic fungus, Fusarium graminearum In the in planta F. graminearum inoculation experiment, we demonstrated that TMV infection of either the plant or the fungus enabled the horizontal transfer of CHV1 from the fungus to the plant, whereas CHV1 infection enhanced fungal acquisition of TMV. Our results demonstrate two-way facilitative interactions between the plant and fungal viruses that promote cross-kingdom virus infections and suggest the presence of plant-fungal-mediated routes for dissemination of fungal and plant viruses in nature.

    DOI: 10.1073/pnas.1915996117

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  • Virome Analysis of Aphid Populations That Infest the Barley Field: The Discovery of Two Novel Groups of Nege/Kita-Like Viruses and Other Novel RNA Viruses. 査読 国際誌

    Hideki Kondo, Miki Fujita, Hiroshi Hisano, Kiwamu Hyodo, Ida Bagus Andika, Nobuhiro Suzuki

    Frontiers in microbiology   11   509 - 509   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aphids (order Hemiptera) are important insect pests of crops and are also vectors of many plant viruses. However, little is known about aphid-infecting viruses, particularly their diversity and relationship to plant viruses. To investigate the aphid viromes, we performed deep sequencing analyses of the aphid transcriptomes from infested barley plants in a field in Japan. We discovered virus-like sequences related to nege/kita-, flavi-, tombus-, phenui-, mononega-, narna-, chryso-, partiti-, and luteoviruses. Using RT-PCR and sequence analyses, we determined almost complete sequences of seven nege/kitavirus-like virus genomes; one of which was a variant of the Wuhan house centipede virus (WHCV-1). The other six seem to belong to four novel viruses distantly related to Wuhan insect virus 9 (WhIV-9) or Hubei nege-like virus 4 (HVLV-4). We designated the four viruses as barley aphid RNA virus 1 to 4 (BARV-1 to -4). Moreover, some nege/kitavirus-like sequences were found by searches on the transcriptome shotgun assembly (TSA) libraries of arthropods and plants. Phylogenetic analyses showed that BARV-1 forms a clade with WHCV-1 and HVLV-4, whereas BARV-2 to -4 clustered with WhIV-9 and an aphid virus, Aphis glycines virus 3. Both virus groups (tentatively designated as Centivirus and Aphiglyvirus, respectively), together with arthropod virus-like TSAs, fill the phylogenetic gaps between the negeviruses and kitaviruses lineages. We also characterized the flavi/jingmen-like and tombus-like virus sequences as well as other RNA viruses, including six putative novel viruses, designated as barley aphid RNA viruses 5 to 10. Interestingly, we also discovered that some aphid-associated viruses, including nege/kita-like viruses, were present in different aphid species, raising a speculation that these viruses might be distributed across different aphid species with plants being the reservoirs. This study provides novel information on the diversity and spread of nege/kitavirus-related viruses and other RNA viruses that are associated with aphids.

    DOI: 10.3389/fmicb.2020.00509

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  • Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes). 査読

    Lin YH, Fujita M, Chiba S, Hyodo K, Andika IB, Suzuki N, Kondo H

    Virology   533   125 - 136   2019年7月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.virol.2019.05.008

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  • Symptomatic plant viroid infections in phytopathogenic fungi. 査読

    Wei S, Bian R, Andika IB, Niu E, Liu Q, Kondo H, Yang L, Zhou H, Pang T, Lian Z, Liu X, Wu Y, Sun L

    Proceedings of the National Academy of Sciences of the United States of America   116 ( 26 )   13042 - 13050   2019年6月

  • A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes. 査読

    Kondo H, Chiba S, Maruyama K, Andika IB, Suzuki N

    Virus research   262   37 - 47   2019年3月

  • Dicer functions transcriptionally and posttranscriptionally in a multilayer antiviral defense. 査読

    Andika IB, Kondo H, Suzuki N

    Proceedings of the National Academy of Sciences of the United States of America   116 ( 6 )   2274 - 2281   2019年2月

  • Coat protein of Chinese wheat mosaic virus upregulates and interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenase, a negative regulator of plant autophagy, to promote virus infection. 国際誌

    Erbo Niu, Chaozheng Ye, Wanying Zhao, Hideki Kondo, Yunfeng Wu, Jianping Chen, Ida Bagus Andika, Liying Sun

    Journal of integrative plant biology   2022年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Autophagy is an intracellular degradation mechanism involved in antiviral defense, but the strategies employed by plant viruses to counteract autophagy-related defense remain unknown for the majority of the viruses. Herein, we describe how the Chinese wheat mosaic virus (CWMV, genus Furovirus) interferes with autophagy and enhances its infection in Nicotiana benthamiana. Yeast two-hybrid screening and in vivo/in vitro assays revealed that the 19 kDa coat protein (CP19K) of CWMV interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenases (GAPCs), negative regulators of autophagy, which bind autophagy-related protein 3 (ATG3), a key factor in autophagy. CP19K also directly interacts with ATG3, possibly leading to the formation of a CP19K-GAPC-ATG3 complex. CP19K-GAPC interaction appeared to intensify CP19K-ATG3 binding. Moreover, CP19K expression upregulated GAPC gene transcripts and reduced autophagic activities. Accordingly, the silencing of GAPC genes in transgenic N. benthamiana reduced CWMV accumulation, whereas CP19K overexpression enhanced it. Overall, our results suggest that CWMV CP19K interferes with autophagy through the promotion and utilization of the GAPC role as a negative regulator of autophagy. This article is protected by copyright. All rights reserved.

    DOI: 10.1111/jipb.13313

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  • ICTV Virus Taxonomy Profile: Rhabdoviridae 2022. 国際誌

    Peter J Walker, Juliana Freitas-Astúa, Nicolas Bejerman, Kim R Blasdell, Rachel Breyta, Ralf G Dietzgen, Anthony R Fooks, Hideki Kondo, Gael Kurath, Ivan V Kuzmin, Pedro Luis Ramos-González, Mang Shi, David M Stone, Robert B Tesh, Noël Tordo, Nikos Vasilakis, Anna E Whitfield, Ictv Report Consortium

    The Journal of general virology   103 ( 6 )   2022年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The family Rhabdoviridae comprises viruses with negative-sense (-) RNA genomes of 10-16 kb. Virions are typically enveloped with bullet-shaped or bacilliform morphology but can also be non-enveloped filaments. Rhabdoviruses infect plants or animals, including mammals, birds, reptiles, amphibians or fish, as well as arthropods, which serve as single hosts or act as biological vectors for transmission to animals or plants. Rhabdoviruses include important pathogens of humans, livestock, fish or agricultural crops. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Rhabdoviridae, which is available at ictv.global/report/rhabdoviridae.

    DOI: 10.1099/jgv.0.001689

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  • A novel deltapartitivirus from red clover. 国際誌

    Paul Telengech, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki

    Archives of virology   167 ( 4 )   1201 - 1204   2022年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The family Partitiviridae has five genera, among which is the genus Deltapartitivirus. We report here the complete genome sequence of a deltapartitivirus from red clover, termed "red clover cryptic virus 3" (RCCV3). RCCV3 has a bisegmented double-stranded (ds) RNA genome. dsRNA1 and dsRNA2 are 1580 and 1589 nucleotides (nt) in length and are predicted to encode an RNA-directed RNA polymerase (RdRP) and a capsid protein (CP), respectively. The RCCV3 RdRP shares the highest sequence identity with the RdRP of a previously reported deltapartitivirus, Medicago sativa deltapartitivirus 1 (MsDPV1) (76.5%), while the RCCV3 CP shows 50% sequence identity to the CP of MsDPV1. RdRP- and CP-based phylogenetic trees place RCCV3 into a clade of deltapartitiviruses. The sequence and phylogenetic analyses clearly indicate that RCCV3 represents a new species in the genus Deltapartitivirus. RCCV3 was detectable in all three tested cultivars of red clover.

    DOI: 10.1007/s00705-022-05372-3

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  • A novel victorivirus from the phytopathogenic fungus Neofusicoccum parvum. 国際誌

    Haris Ahmed Khan, Yukiyo Sato, Hideki Kondo, Atif Jamal, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of virology   167 ( 3 )   923 - 929   2022年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Neofusicoccum parvum is an important plant-pathogenic ascomycetous fungus that causes trunk diseases in a variety of plants. A limited number of reports on mycoviruses from this fungus are available. Here, we report the characterization of a novel victorivirus, Neofusicoccum parvum victorivirus 3 (NpVV3). An agarose gel dsRNA profile of a Pakistani strain of N. parvum, NFN, showed a band of ~5 kbp that was not detectable in Japanese strains of N. parvum. Taking a high-throughput and Sanger sequencing approach, the complete genome sequence of NpVV3 was determined to be 5226 bp in length with two open reading frames (ORF1 and ORF2) that encode a capsid protein (CP) and an RNA-dependent RNA polymerase (RdRP). The RdRP appears to be translated by a stop/restart mechanism facilitated by the junction sequence AUGucUGA, as is found in some other victoriviruses. BLASTp searches showed that NpVV3 CP and RdRP share the highest amino acid sequence identity (80.5% and 72.4%, respectively) with the corresponding proteins of NpVV1 isolated from a French strain of N. parvum. However, NpVV3 was found to be different from NpVV1 in its terminal sequences and the stop/restart facilitator sequence. NpVV3 particles ~35 nm in diameter were partially purified and used to infect an antiviral-RNA-silencing-deficient strain (∆dcl2) of an experimental ascomycetous fungal host, Cryphonectria parasitica. NpVV3 showed symptomless infection in the new host strain.

    DOI: 10.1007/s00705-021-05304-7

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  • A new tetra-segmented splipalmivirus with divided RdRP domains from Cryphonectria naterciae, a fungus found on chestnut and cork oak trees in Europe. 国際誌

    Yukiyo Sato, Sabitree Shahi, Paul Telengech, Sakae Hisano, Carolina Cornejo, Daniel Rigling, Hideki Kondo, Nobuhiro Suzuki

    Virus research   307   198606 - 198606   2022年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Positive-sense (+), single-stranded (ss) RNA viruses with divided RNA-dependent RNA polymerase (RdRP) domains have been reported from diverse filamentous ascomycetes since 2020. These viruses are termed splipalmiviruses or polynarnaviruses and have been characterized largely at the sequence level, but ill-defined biologically. Cryphonectria naterciae, from which only one virus has been reported, is an ascomycetous fungus potentially plant-pathogenic to chestnut and oak trees. We molecularly characterized multiple viruses in a single Portuguese isolate (C0614) of C. naterciae, taking a metatranscriptomic and conventional double-stranded RNA approach. Among them are a novel splipalmivirus (Cryphonectria naterciae splipalmivirus 1, CnSpV1) and a novel fusagravirus (Cryphonectria naterciae fusagravirus 1, CnFGV1). This study focused on the former virus. CnSpV1 has a tetra-segmented, (+)ssRNA genome (RNA1 to RNA4). As observed for other splipalmiviruses reported in 2020 and 2021, the RNA-dependent RNA polymerase domain is separately encoded by RNA1 (motifs F, A and B) and RNA2 (motifs C and D). A hypothetical protein encoded by the 5'-proximal open reading frame of RNA3 shows similarity to a counterpart conserved in some splipalmiviruses. The other RNA3-encoded protein and RNA4-encoded protein show no similarity with known proteins in a blastp search. The tetra-segment nature was confirmed by the conserved terminal sequences of the four CnSpV1 segments (RNA1 to RNA4) and their 100% coexistence in over 100 single conidial isolates tested. The experimental introduction of CnSpV1 along with CnFGV1 into a virus free strain C0754 of C. naterciae vegetatively incompatible with C0614 resulted in no phenotypic alteration, suggesting asymptomatic infection. The protoplast fusion assay indicates a considerably narrow host range of CnSpV1, restricted to the species C. naterciae and C. carpinicola. This study contributes to better understanding of the molecular and biological properties of this unique group of viruses.

    DOI: 10.1016/j.virusres.2021.198606

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  • 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. 国際誌

    Jens H Kuhn, Scott Adkins, Bernard R Agwanda, Rim Al Kubrusli, Sergey V Alkhovsky, Gaya K Amarasinghe, Tatjana Avšič-Županc, María A Ayllón, Justin Bahl, Anne Balkema-Buschmann, Matthew J Ballinger, Christopher F Basler, Sina Bavari, Martin Beer, Nicolas Bejerman, Andrew J Bennett, Dennis A Bente, Éric Bergeron, Brian H Bird, Carol D Blair, Kim R Blasdell, Dag-Ragnar Blystad, Jamie Bojko, Wayne B Borth, Steven Bradfute, Rachel Breyta, Thomas Briese, Paul A Brown, Judith K Brown, Ursula J Buchholz, Michael J Buchmeier, Alexander Bukreyev, Felicity Burt, Carmen Büttner, Charles H Calisher, Mengji Cao, Inmaculada Casas, Kartik Chandran, Rémi N Charrel, Qi Cheng, Yuya Chiaki, Marco Chiapello, Il-Ryong Choi, Marina Ciuffo, J Christopher S Clegg, Ian Crozier, Elena Dal Bó, Juan Carlos de la Torre, Xavier de Lamballerie, Rik L de Swart, Humberto Debat, Nolwenn M Dheilly, Emiliano Di Cicco, Nicholas Di Paola, Francesco Di Serio, Ralf G Dietzgen, Michele Digiaro, Olga Dolnik, Michael A Drebot, J Felix Drexler, William G Dundon, W Paul Duprex, Ralf Dürrwald, John M Dye, Andrew J Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Hugh W Ferguson, Anthony R Fooks, Marco Forgia, Pierre B H Formenty, Jana Fránová, Juliana Freitas-Astúa, Jingjing Fu, Stephanie Fürl, Selma Gago-Zachert, George Fú Gāo, María Laura García, Adolfo García-Sastre, Aura R Garrison, Thomas Gaskin, Jean-Paul J Gonzalez, Anthony Griffiths, Tony L Goldberg, Martin H Groschup, Stephan Günther, Roy A Hall, John Hammond, Tong Han, Jussi Hepojoki, Roger Hewson, Jiang Hong, Ni Hong, Seiji Hongo, Masayuki Horie, John S Hu, Tao Hu, Holly R Hughes, Florian Hüttner, Timothy H Hyndman, M Ilyas, Risto Jalkanen, Dàohóng Jiāng, Gilda B Jonson, Sandra Junglen, Fujio Kadono, Karia H Kaukinen, Michael Kawate, Boris Klempa, Jonas Klingström, Gary Kobinger, Igor Koloniuk, Hideki Kondō, Eugene V Koonin, Mart Krupovic, Kenji Kubota, Gael Kurath, Lies Laenen, Amy J Lambert, Stanley L Langevin, Benhur Lee, Elliot J Lefkowitz, Eric M Leroy, Shaorong Li, Longhui Li, Jiànróng Lǐ, Huazhen Liu, Igor S Lukashevich, Piet Maes, William Marciel de Souza, Marco Marklewitz, Sergio H Marshall, Shin-Yi L Marzano, Sebastien Massart, John W McCauley, Michael Melzer, Nicole Mielke-Ehret, Kristina M Miller, Tobi J Ming, Ali Mirazimi, Gideon J Mordecai, Hans-Peter Mühlbach, Elke Mühlberger, Rayapati Naidu, Tomohide Natsuaki, José A Navarro, Sergey V Netesov, Gabriele Neumann, Norbert Nowotny, Márcio R T Nunes, Alejandro Olmedo-Velarde, Gustavo Palacios, Vicente Pallás, Bernadett Pályi, Anna Papa, Sofia Paraskevopoulou, Adam C Park, Colin R Parrish, David A Patterson, Alex Pauvolid-Corrêa, Janusz T Pawęska, Susan Payne, Carlotta Peracchio, Daniel R Pérez, Thomas S Postler, Liying Qi, Sheli R Radoshitzky, Renato O Resende, Carina A Reyes, Bertus K Rima, Gabriel Robles Luna, Víctor Romanowski, Paul Rota, Dennis Rubbenstroth, Luisa Rubino, Jonathan A Runstadler, Sead Sabanadzovic, Amadou Alpha Sall, Maria S Salvato, Rosemary Sang, Takahide Sasaya, Angela D Schulze, Martin Schwemmle, Mang Shi, Xiǎohóng Shí, Zhènglì Shí, Yoshifumi Shimomoto, Yukio Shirako, Stuart G Siddell, Peter Simmonds, Manuela Sironi, Guy Smagghe, Sophie Smither, Jin-Won Song, Kirsten Spann, Jessica R Spengler, Mark D Stenglein, David M Stone, Jari Sugano, Curtis A Suttle, Amy Tabata, Ayato Takada, Shigeharu Takeuchi, David P Tchouassi, Amy Teffer, Robert B Tesh, Natalie J Thornburg, Yasuhiro Tomitaka, Keizō Tomonaga, Noël Tordo, Baldwyn Torto, Jonathan S Towner, Shinya Tsuda, Changchun Tu, Massimo Turina, Ioannis E Tzanetakis, Janice Uchida, Tomio Usugi, Anna Maria Vaira, Marta Vallino, Bernadette van den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Susanne von Bargen, Jiro Wada, Victoria Wahl, Peter J Walker, Lin-Fa Wang, Guoping Wang, Yanxiang Wang, Yaqin Wang, Muhammad Waqas, Tàiyún Wèi, Shaohua Wen, Anna E Whitfield, John V Williams, Yuri I Wolf, Jiangxiang Wu, Lei Xu, Hironobu Yanagisawa, Caixia Yang, Zuokun Yang, F Murilo Zerbini, Lifeng Zhai, Yong-Zhen Zhang, Song Zhang, Jinguo Zhang, Zhe Zhang, Xueping Zhou

    Archives of virology   166 ( 12 )   3513 - 3566   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.

    DOI: 10.1007/s00705-021-05143-6

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  • Assessment of mycoviral diversity in Pakistani fungal isolates revealed infection by 11 novel viruses of a single strain of Fusarium mangiferae isolate SP1. 国際誌

    Haris Ahmed Khan, Wajeeha Shamsi, Atif Jamal, Memoona Javaied, Mashal Sadiq, Tehsin Fatma, Aqeel Ahmed, Maleeha Arshad, Mubashra Waseem, Samra Babar, Midhat Mustafa Dogar, Nasar Virk, Hussnain Ahmed Janjua, Hideki Kondo, Nobuhiro Suzuki, Muhammad Faraz Bhatti

    The Journal of general virology   102 ( 12 )   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    An extensive screening survey was conducted on Pakistani filamentous fungal isolates for the identification of viral infections. A total of 396 fungal samples were screened, of which 36 isolates were found double-stranded (ds) RNA positive with an overall frequency of 9% when analysed by a classical dsRNA isolation method. One of 36 dsRNA-positive strains, strain SP1 of a plant pathogenic fungus Fusarium mangiferae, was subjected to virome analysis. Next-generation sequencing and subsequent completion of the entire genome sequencing by a classical Sanger sequencing method showed the SP1 strain to be co-infected by 11 distinct viruses, at least seven of which should be described as new taxa at the species level according to the ICTV (International Committee on Taxonomy of Viruses) species demarcation criteria. The newly identified F. mangiferae viruses (FmVs) include two partitivirids, one betapartitivirus (FmPV1) and one gammapartitivirus (FmPV2); six mitovirids, three unuamitovirus (FmMV2, FmMV4, FmMV6), one duamitovirus (FmMV5), and two unclassified mitovirids (FmMV1, FmMV3); and three botourmiavirids, two magoulivirus (FmBOV1, FmBOV3) and one scleroulivirus (FmBOV2). The number of coinfecting viruses is among the largest ones of fungal coinfections. Their molecular features are thoroughly described here. This represents the first large virus survey in the Indian sub-continent.

    DOI: 10.1099/jgv.0.001690

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  • Correction to: 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. 国際誌

    Jens H Kuhn, Scott Adkins, Bernard R Agwanda, Rim Al Kubrusli, Sergey V Alkhovsky, Gaya K Amarasinghe, Tatjana Avšič-Županc, María A Ayllón, Justin Bahl, Anne Balkema-Buschmann, Matthew J Ballinger, Christopher F Basler, Sina Bavari, Martin Beer, Nicolas Bejerman, Andrew J Bennett, Dennis A Bente, Éric Bergeron, Brian H Bird, Carol D Blair, Kim R Blasdell, Dag-Ragnar Blystad, Jamie Bojko, Wayne B Borth, Steven Bradfute, Rachel Breyta, Thomas Briese, Paul A Brown, Judith K Brown, Ursula J Buchholz, Michael J Buchmeier, Alexander Bukreyev, Felicity Burt, Carmen Büttner, Charles H Calisher, Mengji Cao, Inmaculada Casas, Kartik Chandran, Rémi N Charrel, Qi Cheng, Yuya Chiaki, Marco Chiapello, Il-Ryong Choi, Marina Ciuffo, J Christopher S Clegg, Ian Crozier, Elena Dal Bó, Juan Carlos de la Torre, Xavier de Lamballerie, Rik L de Swart, Humberto Debat, Nolwenn M Dheilly, Emiliano Di Cicco, Nicholas Di Paola, Francesco Di Serio, Ralf G Dietzgen, Michele Digiaro, Olga Dolnik, Michael A Drebot, J Felix Drexler, William G Dundon, W Paul Duprex, Ralf Dürrwald, John M Dye, Andrew J Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Hugh W Ferguson, Anthony R Fooks, Marco Forgia, Pierre B H Formenty, Jana Fránová, Juliana Freitas-Astúa, Jingjing Fu, Stephanie Fürl, Selma Gago-Zachert, George Fú Gāo, María Laura García, Adolfo García-Sastre, Aura R Garrison, Thomas Gaskin, Jean-Paul J Gonzalez, Anthony Griffiths, Tony L Goldberg, Martin H Groschup, Stephan Günther, Roy A Hall, John Hammond, Tong Han, Jussi Hepojoki, Roger Hewson, Jiang Hong, Ni Hong, Seiji Hongo, Masayuki Horie, John S Hu, Tao Hu, Holly R Hughes, Florian Hüttner, Timothy H Hyndman, M Ilyas, Risto Jalkanen, Dàohóng Jiāng, Gilda B Jonson, Sandra Junglen, Fujio Kadono, Karia H Kaukinen, Michael Kawate, Boris Klempa, Jonas Klingström, Gary Kobinger, Igor Koloniuk, Hideki Kondō, Eugene V Koonin, Mart Krupovic, Kenji Kubota, Gael Kurath, Lies Laenen, Amy J Lambert, Stanley L Langevin, Benhur Lee, Elliot J Lefkowitz, Eric M Leroy, Shaorong Li, Longhui Li, Jiànróng Lǐ, Huazhen Liu, Igor S Lukashevich, Piet Maes, William Marciel de Souza, Marco Marklewitz, Sergio H Marshall, Shin-Yi L Marzano, Sebastien Massart, John W McCauley, Michael Melzer, Nicole Mielke-Ehret, Kristina M Miller, Tobi J Ming, Ali Mirazimi, Gideon J Mordecai, Hans-Peter Mühlbach, Elke Mühlberger, Rayapati Naidu, Tomohide Natsuaki, José A Navarro, Sergey V Netesov, Gabriele Neumann, Norbert Nowotny, Márcio R T Nunes, Alejandro Olmedo-Velarde, Gustavo Palacios, Vicente Pallás, Bernadett Pályi, Anna Papa, Sofia Paraskevopoulou, Adam C Park, Colin R Parrish, David A Patterson, Alex Pauvolid-Corrêa, Janusz T Pawęska, Susan Payne, Carlotta Peracchio, Daniel R Pérez, Thomas S Postler, Liying Qi, Sheli R Radoshitzky, Renato O Resende, Carina A Reyes, Bertus K Rima, Gabriel Robles Luna, Víctor Romanowski, Paul Rota, Dennis Rubbenstroth, Luisa Rubino, Jonathan A Runstadler, Sead Sabanadzovic, Amadou Alpha Sall, Maria S Salvato, Rosemary Sang, Takahide Sasaya, Angela D Schulze, Martin Schwemmle, Mang Shi, Xiǎohóng Shí, Zhènglì Shí, Yoshifumi Shimomoto, Yukio Shirako, Stuart G Siddell, Peter Simmonds, Manuela Sironi, Guy Smagghe, Sophie Smither, Jin-Won Song, Kirsten Spann, Jessica R Spengler, Mark D Stenglein, David M Stone, Jari Sugano, Curtis A Suttle, Amy Tabata, Ayato Takada, Shigeharu Takeuchi, David P Tchouassi, Amy Teffer, Robert B Tesh, Natalie J Thornburg, Yasuhiro Tomitaka, Keizō Tomonaga, Noël Tordo, Baldwyn Torto, Jonathan S Towner, Shinya Tsuda, Changchun Tu, Massimo Turina, Ioannis E Tzanetakis, Janice Uchida, Tomio Usugi, Anna Maria Vaira, Marta Vallino, Bernadette van den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Susanne von Bargen, Jiro Wada, Victoria Wahl, Peter J Walker, Lin-Fa Wang, Guoping Wang, Yanxiang Wang, Yaqin Wang, Muhammad Waqas, Tàiyún Wèi, Shaohua Wen, Anna E Whitfield, John V Williams, Yuri I Wolf, Jiangxiang Wu, Lei Xu, Hironobu Yanagisawa, Caixia Yang, Zuokun Yang, F Murilo Zerbini, Lifeng Zhai, Yong-Zhen Zhang, Song Zhang, Jinguo Zhang, Zhe Zhang, Xueping Zhou

    Archives of virology   2021年11月

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  • Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand.

    Sokty Neang, Santiti Bincader, Sansern Rangsuwan, Pisut Keawmanee, Soriya Rin, Lakha Salaipeth, Subha Das, Hideki Kondo, Nobuhiro Suzuki, Ikuo Sato, Daigo Takemoto, Chainarong Rattanakreetakul, Ratiya Pongpisutta, Masao Arakawa, Sotaro Chiba

    Viruses   13 ( 11 )   2269   2021年11月

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  • ICTV Virus Taxonomy Profile: Nyamiviridae 2021. 国際誌

    Ralf G Dietzgen, Andrew E Firth, Dàohóng Jiāng, Sandra Junglen, Hideki Kondo, Jens H Kuhn, Sofia Paraskevopoulou, Nikos Vasilakis, Ictv Report Consortium

    The Journal of general virology   102 ( 11 )   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nyamiviridae is a family of viruses in the order Mononegavirales, with unsegmented (except for members of the genus Tapwovirus), negative-sense RNA genomes of 10-13 kb. Nyamviruses have a genome organisation and content similar to that of other mononegaviruses. Nyamiviridae includes several genera that form monophyletic clades on phylogenetic analysis of the RNA polymerase. Nyamiviruses have been found associated with diverse invertebrates as well as land- and seabirds. Members of the genera Nyavirus and Socyvirus produce enveloped, spherical virions. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Nyamiviridae, which is available at ictv.global/report/nyamiviridae.

    DOI: 10.1099/jgv.0.001681

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  • Epidemic progress of beet necrotic yellow vein virus: Evidence from an investigation in Japan spanning half a century

    Ryo Nakagami, Sotaro Chiba, Naoto Yoshida, Yoshiteru Senoo, Minako Iketani-Saito, Satoru Iketani, Hideki Kondo, Tetsuo Tamada

    PLANT PATHOLOGY   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Beet necrotic yellow vein virus (BNYVV) is the causal agent of rhizomania, the most serious sugar beet disease worldwide. Since the first finding in Japan in 1969, BNYVV became widespread throughout Hokkaido in a few decades and led to the introduction of Rz1-resistant sugar beet cultivars in the 1990s. Here, we report the historical progress of the BNYVV epidemic in Hokkaido from 1969 to 2019. Previous analysis on samples from 1991 showed that BNYVV isolates were classified into three strains (named O, D, and T) based on the RNA3-encoded p25 gene. The O-type viruses were widely detected in Hokkaido, while the D- and T-type viruses were detected in limited areas. The RNA5, encoding the p26 gene, was initially contained in some D- and O-type isolates but not in any T-type isolates. Interestingly, recent sample analysis revealed that RNA5-containing T-type viruses, seemingly more virulent than the other two strains, were widely detected in Hokkaido. Additionally, a small group of virus isolates harbouring a new p25 gene (named C) was found in limited areas. These results suggest that the T-type viruses, which accompanied RNA5, have been preferentially spread from a limited area to other districts over the last few decades and that this spread might be strongly associated with the recent introduction of Rz1-resistant sugar beet cultivars. BNYVV-positive samples also contained mainly beet soil-borne virus and traces of beet virus Q, both of which are the first to be recorded in Japan.

    DOI: 10.1111/ppa.13504

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  • A second capsidless hadakavirus strain with 10 positive-sense single-stranded RNA genomic segments from Fusarium nygamai. 国際誌

    Haris Ahmed Khan, Yukiyo Sato, Hideki Kondo, Atif Jamal, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of virology   166 ( 10 )   2711 - 2722   2021年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A unique capsidless virus with a positive-sense, single-stranded RNA genome (hadakavirus 1, HadV1), a member of the extended picorna-like supergroup, was isolated previously from the phytopathogenic fungus Fusarium oxysporum. Here, we describe the molecular and biological characterisation of a second hadakavirus strain from Fusarium nygamai, which has not been investigated in detail previously as a virus host. This virus, hadakavirus 1 strain 1NL (HadV1-1NL), has features similar to the first hadakavirus, HadV1-7n, despite having a different number of segments (10 for HadV1-1NL vs. 11 for HadV1-7n). The 10 genomic RNA segments of HadV1-1NL range in size from 0.9 kb to 2.5 kb. All HadV1-1NL segments show 67% to 86% local nucleotide sequence identity to their HadV1-7n counterparts, whereas HadV1-1NL has no homolog of HadV1-7n RNA8, which encodes a zinc-finger motif. Another interesting feature is the possible coding incapability of HadV1-1NL RNA10. HadV1-1NL was predicted to be capsidless based on the RNase A susceptibility of its replicative form dsRNA. Phenotypic comparison of multiple virus-infected and virus-free single-spore isolates indicated asymptomatic infection by HadV1-1NL. Less-efficient vertical transmission via spores was observed as the infected fungal colonies from which the spores were derived became older, as was observed for HadV1-7n. This study shows a second example of a hadakavirus that appears to have unusual features.

    DOI: 10.1007/s00705-021-05176-x

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  • Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range. 国際誌

    Sabitree Shahi, Sotaro Chiba, Hideki Kondo, Nobuhiro Suzuki

    Virology   554   55 - 65   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cryphonectria nitschkei chrysovirus 1 (CnCV1), was described earlier from an ascomycetous fungus, Cryphonectria nitschkei strain OB5/11, collected in Japan; its partial sequence was reported a decade ago. Complete sequencing of the four genomic dsRNA segments revealed molecular features similar to but distinct from previously reported members of the family Chrysoviridae. Unique features include the presence of a mini-cistron preceding the major large open reading frame in each genomic segment. Common features include the presence of CAA repeats in the 5'-untranslated regions and conserved terminal sequences. CnCV1-OB5/11 could be laterally transferred to C. nitschkei and its relatives C. radicalis and C. naterciae via coculturing, virion transfection and protoplast fusion, but not to fungal species other than the three species mentioned above, even within the genus Cryphonectria, suggesting a very narrow host range. Phenotypic comparison of a few sets of CnCV1-infected and -free isogenic strains showed symptomless infection in new hosts.

    DOI: 10.1016/j.virol.2020.11.011

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  • Identification of an RNA Silencing Suppressor Encoded by a Symptomless Fungal Hypovirus, Cryphonectria Hypovirus 4. 国際誌

    Annisa Aulia, Kiwamu Hyodo, Sakae Hisano, Hideki Kondo, Bradley I Hillman, Nobuhiro Suzuki

    Biology   10 ( 2 )   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Previously, we have reported the ability of a symptomless hypovirus Cryphonectria hypovirus 4 (CHV4) of the chestnut blight fungus to facilitate stable infection by a co-infecting mycoreovirus 2 (MyRV2)-likely through the inhibitory effect of CHV4 on RNA silencing (Aulia et al., Virology, 2019). In this study, the N-terminal portion of the CHV4 polyprotein, termed p24, is identified as an autocatalytic protease capable of suppressing host antiviral RNA silencing. Using a bacterial expression system, CHV4 p24 is shown to cleave autocatalytically at the di-glycine peptide (Gly214-Gly215) of the polyprotein through its protease activity. Transgenic expression of CHV4 p24 in Cryphonectria parasitica suppresses the induction of one of the key genes of the antiviral RNA silencing, dicer-like 2, and stabilizes the infection of RNA silencing-susceptible virus MyRV2. This study shows functional similarity between CHV4 p24 and its homolog p29, encoded by the symptomatic prototype hypovirus CHV1.

    DOI: 10.3390/biology10020100

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  • 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. 国際誌

    Jens H Kuhn, Scott Adkins, Daniela Alioto, Sergey V Alkhovsky, Gaya K Amarasinghe, Simon J Anthony, Tatjana Avšič-Županc, María A Ayllón, Justin Bahl, Anne Balkema-Buschmann, Matthew J Ballinger, Tomáš Bartonička, Christopher Basler, Sina Bavari, Martin Beer, Dennis A Bente, Éric Bergeron, Brian H Bird, Carol Blair, Kim R Blasdell, Steven B Bradfute, Rachel Breyta, Thomas Briese, Paul A Brown, Ursula J Buchholz, Michael J Buchmeier, Alexander Bukreyev, Felicity Burt, Nihal Buzkan, Charles H Calisher, Mengji Cao, Inmaculada Casas, John Chamberlain, Kartik Chandran, Rémi N Charrel, Biao Chen, Michela Chiumenti, Il-Ryong Choi, J Christopher S Clegg, Ian Crozier, John V da Graça, Elena Dal Bó, Alberto M R Dávila, Juan Carlos de la Torre, Xavier de Lamballerie, Rik L de Swart, Patrick L Di Bello, Nicholas Di Paola, Francesco Di Serio, Ralf G Dietzgen, Michele Digiaro, Valerian V Dolja, Olga Dolnik, Michael A Drebot, Jan Felix Drexler, Ralf Dürrwald, Lucie Dufkova, William G Dundon, W Paul Duprex, John M Dye, Andrew J Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Jorlan Fernandes, Anthony R Fooks, Pierre B H Formenty, Leonie F Forth, Ron A M Fouchier, Juliana Freitas-Astúa, Selma Gago-Zachert, George Fú Gāo, María Laura García, Adolfo García-Sastre, Aura R Garrison, Aiah Gbakima, Tracey Goldstein, Jean-Paul J Gonzalez, Anthony Griffiths, Martin H Groschup, Stephan Günther, Alexandro Guterres, Roy A Hall, John Hammond, Mohamed Hassan, Jussi Hepojoki, Satu Hepojoki, Udo Hetzel, Roger Hewson, Bernd Hoffmann, Seiji Hongo, Dirk Höper, Masayuki Horie, Holly R Hughes, Timothy H Hyndman, Amara Jambai, Rodrigo Jardim, Dàohóng Jiāng, Qi Jin, Gilda B Jonson, Sandra Junglen, Serpil Karadağ, Karen E Keller, Boris Klempa, Jonas Klingström, Gary Kobinger, Hideki Kondō, Eugene V Koonin, Mart Krupovic, Gael Kurath, Ivan V Kuzmin, Lies Laenen, Robert A Lamb, Amy J Lambert, Stanley L Langevin, Benhur Lee, Elba R S Lemos, Eric M Leroy, Dexin Li, Jiànróng Lǐ, Mifang Liang, Wénwén Liú, Yàn Liú, Igor S Lukashevich, Piet Maes, William Marciel de Souza, Marco Marklewitz, Sergio H Marshall, Giovanni P Martelli, Robert R Martin, Shin-Yi L Marzano, Sébastien Massart, John W McCauley, Nicole Mielke-Ehret, Angelantonio Minafra, Maria Minutolo, Ali Mirazimi, Hans-Peter Mühlbach, Elke Mühlberger, Rayapati Naidu, Tomohide Natsuaki, Beatriz Navarro, José A Navarro, Sergey V Netesov, Gabriele Neumann, Norbert Nowotny, Márcio R T Nunes, Are Nylund, Arnfinn L Økland, Renata C Oliveira, Gustavo Palacios, Vicente Pallas, Bernadett Pályi, Anna Papa, Colin R Parrish, Alex Pauvolid-Corrêa, Janusz T Pawęska, Susan Payne, Daniel R Pérez, Florian Pfaff, Sheli R Radoshitzky, Aziz-Ul Rahman, Pedro L Ramos-González, Renato O Resende, Carina A Reyes, Bertus K Rima, Víctor Romanowski, Gabriel Robles Luna, Paul Rota, Dennis Rubbenstroth, Jonathan A Runstadler, Daniel Ruzek, Sead Sabanadzovic, Jiří Salát, Amadou Alpha Sall, Maria S Salvato, Kamil Sarpkaya, Takahide Sasaya, Martin Schwemmle, Muhammad Z Shabbir, Xiǎohóng Shí, Zhènglì Shí, Yukio Shirako, Peter Simmonds, Jana Širmarová, Manuela Sironi, Sophie Smither, Teemu Smura, Jin-Won Song, Kirsten M Spann, Jessica R Spengler, Mark D Stenglein, David M Stone, Petra Straková, Ayato Takada, Robert B Tesh, Natalie J Thornburg, Keizō Tomonaga, Noël Tordo, Jonathan S Towner, Massimo Turina, Ioannis Tzanetakis, Rainer G Ulrich, Anna Maria Vaira, Bernadette van den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Victoria Wahl, Peter J Walker, Hui Wang, Jianwei Wang, Xifeng Wang, Lin-Fa Wang, Tàiyún Wèi, Heather Wells, Anna E Whitfield, John V Williams, Yuri I Wolf, Zhìqiáng Wú, Xin Yang, Xīnglóu Yáng, Xuejie Yu, Natalya Yutin, F Murilo Zerbini, Tong Zhang, Yong-Zhen Zhang, Guohui Zhou, Xueping Zhou

    Archives of virology   165 ( 12 )   3023 - 3072   2020年12月

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    記述言語:英語  

    In March 2020, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. At the genus rank, 20 new genera were added, two were deleted, one was moved, and three were renamed. At the species rank, 160 species were added, four were deleted, ten were moved and renamed, and 30 species were renamed. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.

    DOI: 10.1007/s00705-020-04731-2

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  • Pathogenetic roles of beet necrotic yellow vein virus RNA5 in the exacerbation of symptoms and yield reduction, development of scab-like symptoms, andRz1-resistance breaking in sugar beet

    Tetsuo Tamada, Hirokatsu Uchino, Toshimi Kusume, Minako Iketani-Saito, Sotaro Chiba, Ida Bagus Andika, Hideki Kondo

    PLANT PATHOLOGY   70 ( 1 )   219 - 232   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Beet necrotic yellow vein virus (BNYVV) generally has a four-segmented positive-sense RNA genome (RNAs 1-4), but some European and most Asian strains have an additional segment, RNA5. This study examined the effect of RNA5 and RNA3 on different sugar beet cultivars using aPolymyxa-mediated inoculation system under field and laboratory conditions. In field tests, the degree of sugar yield served as an index for assessing the virulence of BNYVV strains. Japanese A-II type isolates without RNA5 caused mostly 15%-90% sugar yield reductions, depending on the susceptibility of sugar beet cultivars, whereas the isolates with RNA5 induced more than 90% yield losses in the seven susceptible cultivars, but small yield losses in oneRz1-resistant and Rizor cultivars. However, a laboratory-produced isolate containing RNA5 but lacking RNA3 caused higher yield losses in Rizor than in susceptible plants, and induced scab-like symptoms on the root surface of both susceptible and resistant plants. In laboratory tests, A-II type isolates without RNA5 had low viral RNA accumulation levels in roots of Rizor andRz1-resistant plants at early stages of infection, but in the presence of RNA5, viral RNA3 accumulation levels increased remarkably. This increased RNA3 accumulation was not observed in roots of the WB42 accession with theRz2gene. In contrast, the presence of RNA3 did not affect RNA5 accumulation levels. Collectively, this study demonstrated that RNA5 is involved in the development of scab-like symptoms and the enhancement of RNA3 accumulation, and suggests these characteristics of RNA5 are associated withRz1-resistance breaking.

    DOI: 10.1111/ppa.13266

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  • Hadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, Fusarium oxysporum. 査読 国際誌

    Yukiyo Sato, Wajeeha Shamsi, Atif Jamal, Muhammad Faraz Bhatti, Hideki Kondo, Nobuhiro Suzuki

    mBio   11 ( 3 )   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The search for viruses infecting fungi, or mycoviruses, has extended our knowledge about the diversity of RNA viruses, as exemplified by the discovery of polymycoviruses, a phylogenetic group of multisegmented RNA viruses with unusual forms. The genomic RNAs of known polymycoviruses, which show a phylogenetic affinity for animal positive-sense single-stranded RNA [(+)RNA] viruses such as caliciviruses, are comprised of four conserved segments with an additional zero to four segments. The double-stranded form of polymycovirus genomic RNA is assumed to be associated with a virally encoded protein (proline-alanine-serine-rich protein [PASrp]) in either of two manners: a capsidless colloidal form or a filamentous encapsidated form. Detailed molecular characterizations of polymycoviruses, however, have been conducted for only a few strains. Here, a novel polymyco-related virus named Hadaka virus 1 (HadV1), from the phytopathogenic fungus Fusarium oxysporum, was characterized. The genomic RNA of HadV1 consisted of an 11-segmented positive-sense RNA with highly conserved terminal nucleotide sequences. HadV1 shared the three conserved segments with known polymycoviruses but lacked the PASrp-encoding segment. Unlike the known polymycoviruses and encapsidated viruses, HadV1 was not pelleted by conventional ultracentrifugation, possibly due to the lack of PASrp. This result implied that HadV1 exists only as a soluble form with naked RNA. Nevertheless, the 11 genomic segments of HadV1 have been stably maintained through host subculturing and conidiation. Taken together, the results of this study revealed a virus with a potential novel virus lifestyle, carrying many genomic segments without typical capsids or PASrp-associated forms.IMPORTANCE Fungi collectively host various RNA viruses. Examples include encapsidated double-stranded RNA (dsRNA) viruses with diverse numbers of genomic segments (from 1 to 12) and capsidless viruses with nonsegmented (+)RNA genomes. Recently, viruses with unusual intermediate features of an infectious entity between encapsidated dsRNA viruses and capsidless (+)RNA viruses were found. They are called polymycoviruses, which typically have four to eight dsRNA genomic segments associated with one of the virus-encoded proteins and are phylogenetically distantly related to animal (+)RNA caliciviruses. Here, we identified a novel virus phylogenetically related to polymycoviruses, from the phytopathogenic fungus Fusarium oxysporum The virus, termed Hadaka virus 1 (HadV1), has 11 (+)RNA genomic segments, the largest number in known (+)RNA viruses. Nevertheless, HadV1 lacked a typical structural protein of polymycoviruses and was not pelleted by standard ultracentrifugation, implying an unusual capsidless nature of HadV1. This study reveals a potential novel lifestyle of multisegmented RNA viruses.

    DOI: 10.1128/mBio.00450-20

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  • Reply to Serra et al.: Nucleotide substitutions in plant viroid genomes that multiply in phytopathogenic fungi. 査読 国際誌

    Shuang Wei, Ruiling Bian, Ida Bagus Andika, Erbo Niu, Qian Liu, Hideki Kondo, Liu Yang, Hongsheng Zhou, Tianxing Pang, Ziqian Lian, Xili Liu, Yunfeng Wu, Liying Sun

    Proceedings of the National Academy of Sciences of the United States of America   117 ( 19 )   10129 - 10130   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1073/pnas.2001670117

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  • Diversity and epidemiology of plant rhabdoviruses. 国際誌

    Ralf G Dietzgen, Nicolas E Bejerman, Michael M Goodin, Colleen M Higgins, Ordom B Huot, Hideki Kondo, Kathleen M Martin, Anna E Whitfield

    Virus research   281   197942 - 197942   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Plant rhabdoviruses are recognized by their large bacilliform particles and for being able to replicate in both their plant hosts and arthropod vectors. This review highlights selected, better studied examples of plant rhabdoviruses, their genetic diversity, epidemiology and interactions with plant hosts and arthropod vectors: Alfalfa dwarf virus is classified as a cytorhabdovirus, but its multifunctional phosphoprotein is localized to the plant cell nucleus. Lettuce necrotic yellows virus subtypes may differentially interact with their aphid vectors leading to changes in virus population diversity. Interactions of rhabdoviruses that infect rice, maize and other grains are tightly associated with their specific leafhopper and planthopper vectors. Future outbreaks of vector-borne nucleorhabdoviruses may be predicted based on a world distribution map of the insect vectors. The epidemiology of coffee ringspot virus and its Brevipalpus mite vector is illustrated highlighting the symptomatology and biology of a dichorhavirus and potential impacts of climate change on its epidemiology.

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  • Molecular Characterization of a Novel Polymycovirus From Penicillium janthinellum With a Focus on Its Genome-Associated PASrp. 国際誌

    Yukiyo Sato, Atif Jamal, Hideki Kondo, Nobuhiro Suzuki

    Frontiers in microbiology   11   592789 - 592789   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The genus Polymycovirus of the family Polymycoviridae accommodates fungal RNA viruses with different genomic segment numbers (four, five, or eight). It is suggested that four members form no true capsids and one forms filamentous virus particles enclosing double-stranded RNA (dsRNA). In both cases, viral dsRNA is associated with a viral protein termed "proline-alanine-serine-rich protein" (PASrp). These forms are assumed to be the infectious entity. However, the detailed molecular characteristics of PASrps remain unclear. Here, we identified a novel five-segmented polymycovirus, Penicillium janthinellum polymycovirus 1 (PjPmV1), and characterized its purified fraction form in detail. The PjPmV1 had five dsRNA segments associated with PASrp. Density gradient ultracentrifugation of the PASrp-associated PjPmV1 dsRNA revealed its uneven structure and a broad fractionation profile distinct from that of typical encapsidated viruses. Moreover, PjPmV1-PASrp interacted in vitro with various nucleic acids in a sequence-non-specific manner. These PjPmV1 features are discussed in view of the diversification of genomic segment numbers of the genus Polymycovirus.

    DOI: 10.3389/fmicb.2020.592789

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  • Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix. 査読 国際誌

    Paul Telengech, Sakae Hisano, Cyrus Mugambi, Kiwamu Hyodo, Juan Manuel Arjona-López, Carlos José López-Herrera, Satoko Kanematsu, Hideki Kondo, Nobuhiro Suzuki

    Frontiers in microbiology   11   1064 - 1064   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report the characterization of a total of 20 partitiviruses from 16 R. necatrix strains belonging to 15 new species, for which "Rosellinia necatrix partitivirus 11-Rosellinia necatrix partitivirus 25" were proposed, and 5 previously reported species. The newly identified partitiviruses have been taxonomically placed in two genera, Alphapartitivirus, and Betapartitivirus. Some partitiviruses were transfected into reference strains of the natural host, R. necatrix, and an experimental host, Cryphonectria parasitica, using purified virions. A comparative analysis of resultant transfectants revealed interesting differences and similarities between the RNA accumulation and symptom induction patterns of R. necatrix and C. parasitica. Other interesting findings include the identification of a probable reassortment event and a quintuple partitivirus infection of a single fungal strain. These combined results provide a foundation for further studies aimed at elucidating mechanisms that underly the differences observed.

    DOI: 10.3389/fmicb.2020.01064

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  • A symptomless hypovirus, CHV4, facilitates stable infection of the chestnut blight fungus by a coinfecting reovirus likely through suppression of antiviral RNA silencing. 査読

    Aulia A, Andika IB, Kondo H, Hillman BI, Suzuki N

    Virology   533   99 - 107   2019年7月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.virol.2019.05.004

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  • Taxonomy of the order Mononegavirales: update 2019. 国際誌

    Gaya K Amarasinghe, María A Ayllón, Yīmíng Bào, Christopher F Basler, Sina Bavari, Kim R Blasdell, Thomas Briese, Paul A Brown, Alexander Bukreyev, Anne Balkema-Buschmann, Ursula J Buchholz, Camila Chabi-Jesus, Kartik Chandran, Chiara Chiapponi, Ian Crozier, Rik L de Swart, Ralf G Dietzgen, Olga Dolnik, Jan F Drexler, Ralf Dürrwald, William G Dundon, W Paul Duprex, John M Dye, Andrew J Easton, Anthony R Fooks, Pierre B H Formenty, Ron A M Fouchier, Juliana Freitas-Astúa, Anthony Griffiths, Roger Hewson, Masayuki Horie, Timothy H Hyndman, Dàohóng Jiāng, Elliott W Kitajima, Gary P Kobinger, Hideki Kondō, Gael Kurath, Ivan V Kuzmin, Robert A Lamb, Antonio Lavazza, Benhur Lee, Davide Lelli, Eric M Leroy, Jiànróng Lǐ, Piet Maes, Shin-Yi L Marzano, Ana Moreno, Elke Mühlberger, Sergey V Netesov, Norbert Nowotny, Are Nylund, Arnfinn L Økland, Gustavo Palacios, Bernadett Pályi, Janusz T Pawęska, Susan L Payne, Alice Prosperi, Pedro Luis Ramos-González, Bertus K Rima, Paul Rota, Dennis Rubbenstroth, Mǎng Shī, Peter Simmonds, Sophie J Smither, Enrica Sozzi, Kirsten Spann, Mark D Stenglein, David M Stone, Ayato Takada, Robert B Tesh, Keizō Tomonaga, Noël Tordo, Jonathan S Towner, Bernadette van den Hoogen, Nikos Vasilakis, Victoria Wahl, Peter J Walker, Lin-Fa Wang, Anna E Whitfield, John V Williams, F Murilo Zerbini, Tāo Zhāng, Yong-Zhen Zhang, Jens H Kuhn

    Archives of virology   164 ( 7 )   1967 - 1980   2019年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In February 2019, following the annual taxon ratification vote, the order Mononegavirales was amended by the addition of four new subfamilies and 12 new genera and the creation of 28 novel species. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).

    DOI: 10.1007/s00705-019-04247-4

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  • Novel Victorivirus from a Pakistani Isolate of Alternaria alternata Lacking a Typical Translational Stop/Restart Sequence Signature. 査読 国際誌

    Jamal A, Sato Y, Shahi S, Shamsi W, Kondo H, Suzuki N

    Viruses   11 ( 6 )   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/v11060577

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  • Isolation and characterization of a novel mycovirus infecting an edible mushroom, Grifola frondosa. 査読

    Akiko Komatsu, Hideki Kondo, Masayuki Sato, Atsushi Kurahashi, Kozo Nishibori, Nobuhiro Suzuki, Fumihiro Fujimori

    Mycoscience   60   211 - 220   2019年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Molecular and biological characterization of a novel botybirnavirus identified from a Pakistani isolate of Alternaria alternata. 査読 国際誌

    Shamsi W, Sato Y, Jamal A, Shahi S, Kondo H, Suzuki N, Bhatti MF

    Virus research   263   119 - 128   2019年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.virusres.2019.01.006

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  • Taxonomy of the order Mononegavirales: second update 2018. 国際誌

    Piet Maes, Gaya K Amarasinghe, María A Ayllón, Christopher F Basler, Sina Bavari, Kim R Blasdell, Thomas Briese, Paul A Brown, Alexander Bukreyev, Anne Balkema-Buschmann, Ursula J Buchholz, Kartik Chandran, Ian Crozier, Rik L de Swart, Ralf G Dietzgen, Olga Dolnik, Leslie L Domier, Jan F Drexler, Ralf Dürrwald, William G Dundon, W Paul Duprex, John M Dye, Andrew J Easton, Anthony R Fooks, Pierre B H Formenty, Ron A M Fouchier, Juliana Freitas-Astúa, Elodie Ghedin, Anthony Griffiths, Roger Hewson, Masayuki Horie, Julia L Hurwitz, Timothy H Hyndman, Dàohóng Jiāng, Gary P Kobinger, Hideki Kondō, Gael Kurath, Ivan V Kuzmin, Robert A Lamb, Benhur Lee, Eric M Leroy, Jiànróng Lǐ, Shin-Yi L Marzano, Elke Mühlberger, Sergey V Netesov, Norbert Nowotny, Gustavo Palacios, Bernadett Pályi, Janusz T Pawęska, Susan L Payne, Bertus K Rima, Paul Rota, Dennis Rubbenstroth, Peter Simmonds, Sophie J Smither, Qisheng Song, Timothy Song, Kirsten Spann, Mark D Stenglein, David M Stone, Ayato Takada, Robert B Tesh, Keizō Tomonaga, Noël Tordo, Jonathan S Towner, Bernadette van den Hoogen, Nikos Vasilakis, Victoria Wahl, Peter J Walker, David Wang, Lin-Fa Wang, Anna E Whitfield, John V Williams, Gōngyín Yè, F Murilo Zerbini, Yong-Zhen Zhang, Jens H Kuhn

    Archives of virology   164 ( 4 )   1233 - 1244   2019年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In October 2018, the order Mononegavirales was amended by the establishment of three new families and three new genera, abolishment of two genera, and creation of 28 novel species. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).

    DOI: 10.1007/s00705-018-04126-4

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  • Investigation of Host Range of and Host Defense against a Mitochondrially Replicating Mitovirus. 査読

    Shahi S, Eusebio-Cope A, Kondo H, Hillman BI, Suzuki N

    Journal of virology   93 ( 6 )   2019年3月

  • Horizontal Transfer of a Retrotransposon from the Rice Planthopper to the Genome of an Insect DNA Virus. 査読

    Yang Q, Zhang Y, Andika IB, Liao Z, Kondo H, Lu Y, Cheng Y, Li L, He Y, He Y, Qi Y, Sun Z, Wu Y, Yan F, Chen J, Li J

    Journal of virology   93 ( 6 )   2019年3月

  • Identification of a Novel Hypovirulence-Inducing Hypovirus From Alternaria alternata. 査読

    Li H, Bian R, Liu Q, Yang L, Pang T, Salaipeth L, Andika IB, Kondo H, Sun L

    Frontiers in microbiology   10   1076   2019年

  • Plant rhabdoviruses-their origins and vector interactions. 国際誌

    Anna E Whitfield, Ordom Brian Huot, Kathleen M Martin, Hideki Kondo, Ralf G Dietzgen

    Current opinion in virology   33   198 - 207   2018年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Classical plant rhabdoviruses infect monocot and dicot plants, have unsegmented negative-sense RNA genomes and have been taxonomically classified in the genera Cytorhabdovirus and Nucleorhabdovirus. These viruses replicate in their hemipteran vectors and are transmitted in a circulative-propagative mode and virus infection persists for the life of the insect. Based on the discovery of numerous novel rhabdoviruses in arthropods during metagenomic studies and extensive phylogenetic analyses of the family Rhabdoviridae, it is hypothesized that plant-infecting rhabdoviruses are derived from insect viruses. Analyses of viral gene function in plants and insects is beginning to reveal conserved and unique biology for these plant viruses in the two diverse hosts. New tools for insect molecular biology and infectious clones for plant rhabdoviruses are increasing our understanding of the lifestyles of these viruses.

    DOI: 10.1016/j.coviro.2018.11.002

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  • Novel Mitoviruses and a Unique Tymo-Like Virus in Hypovirulent and Virulent Strains of the Fusarium Head Blight Fungus, Fusarium boothii. 査読

    Mizutani Y, Abraham A, Uesaka K, Kondo H, Suga H, Suzuki N, Chiba S

    Viruses   10 ( 11 )   2018年10月

  • Taxonomy of the order Mononegavirales: update 2018. 国際誌

    Gaya K Amarasinghe, Nidia G Aréchiga Ceballos, Ashley C Banyard, Christopher F Basler, Sina Bavari, Andrew J Bennett, Kim R Blasdell, Thomas Briese, Alexander Bukreyev, Yíngyún Caì, Charles H Calisher, Cristine Campos Lawson, Kartik Chandran, Colin A Chapman, Charles Y Chiu, Kang-Seuk Choi, Peter L Collins, Ralf G Dietzgen, Valerian V Dolja, Olga Dolnik, Leslie L Domier, Ralf Dürrwald, John M Dye, Andrew J Easton, Hideki Ebihara, Juan E Echevarría, Anthony R Fooks, Pierre B H Formenty, Ron A M Fouchier, Conrad M Freuling, Elodie Ghedin, Tony L Goldberg, Roger Hewson, Masayuki Horie, Timothy H Hyndman, Dàohóng Jiāng, Robert Kityo, Gary P Kobinger, Hideki Kondō, Eugene V Koonin, Mart Krupovic, Gael Kurath, Robert A Lamb, Benhur Lee, Eric M Leroy, Piet Maes, Andrea Maisner, Denise A Marston, Sunil Kumar Mor, Thomas Müller, Elke Mühlberger, Víctor Manuel Neira Ramírez, Sergey V Netesov, Terry Fei Fan Ng, Norbert Nowotny, Gustavo Palacios, Jean L Patterson, Janusz T Pawęska, Susan L Payne, Karla Prieto, Bertus K Rima, Paul Rota, Dennis Rubbenstroth, Martin Schwemmle, Stuart Siddell, Sophie J Smither, Qisheng Song, Timothy Song, Mark D Stenglein, David M Stone, Ayato Takada, Robert B Tesh, Luciano Matsumiya Thomazelli, Keizō Tomonaga, Noël Tordo, Jonathan S Towner, Nikos Vasilakis, Sonia Vázquez-Morón, Claudio Verdugo, Viktor E Volchkov, Victoria Wahl, Peter J Walker, David Wang, Lin-Fa Wang, James F X Wellehan, Michael R Wiley, Anna E Whitfield, Yuri I Wolf, Gōngyín Yè, Yǒng-Zhèn Zhāng, Jens H Kuhn

    Archives of virology   163 ( 8 )   2283 - 2294   2018年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In 2018, the order Mononegavirales was expanded by inclusion of 1 new genus and 12 novel species. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV) and summarizes additional taxonomic proposals that may affect the order in the near future.

    DOI: 10.1007/s00705-018-3814-x

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  • Evidence for a novel negative-stranded RNA mycovirus isolated from the plant pathogenic fungus Fusarium graminearum 査読

    Luan Wang, Hao He, Shuangchao Wang, Xiaoguang Chen, Dewen Qiu, Hideki Kondo, Lihua Guo

    Virology   518   232 - 240   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Academic Press Inc.  

    Here we describe a novel (−)ssRNA mycovirus, Fusarium graminearum negative-stranded RNA virus 1 (FgNSRV-1), isolated from Fusarium graminearum strain HN1. The genome of FgNSRV-1 is 9072 nucleotides in length, with five discontinuous but linear ORFs (ORF I-V). Phylogenetic analysis based on entire L polymerase sequences indicated that FgNSRV-1 is related to the (−)ssRNA mycovirus Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV-1), and other mycoviruses. Our data suggest that FgNSRV-1 can be classified into the family Mymonaviridae, order Mononegavirales. Putative enveloped virion-like structures with filamentous morphology similar to SsNSRV-1 were observed in virion preparation samples. The L proteins of FgNSRV-1, and other fungal mononegaviruses, were found to be related to L protein-like sequences in some fungal genome, supporting the hypothesis that there is coevolution occurring between mycoviruses and fungi. Besides, clearing the virus from the infected host fungus resulted in no discernable phenotypic change.

    DOI: 10.1016/j.virol.2018.03.008

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  • ICTV Virus Taxonomy Profile: Hypoviridae. 国際誌

    Nobuhiro Suzuki, Said A Ghabrial, Kook-Hyung Kim, Michael Pearson, Shin-Yi L Marzano, Hajime Yaegashi, Jiatao Xie, Lihua Guo, Hideki Kondo, Igor Koloniuk, Bradley I Hillman, Ictv Report Consortium

    The Journal of general virology   99 ( 5 )   615 - 616   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The Hypoviridae, comprising one genus, Hypovirus, is a family of capsidless viruses with positive-sense, ssRNA genomes of 9.1-12.7 kb that possess either a single large ORF or two ORFs. The ORFs appear to be translated from genomic RNA by non-canonical mechanisms, i.e. internal ribosome entry site-mediated and stop/restart translation. Hypoviruses have been detected in ascomycetous or basidiomycetous filamentous fungi, and are considered to be replicated in host Golgi-derived, lipid vesicles that contain their dsRNA as a replicative form. Some hypoviruses induce hypovirulence to host fungi, while others do not. This is a summary of the current ICTV report on the taxonomy of the Hypoviridae, which is available at www.ictv.global/report/hypoviridae.

    DOI: 10.1099/jgv.0.001055

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  • Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus, Rosellinia necatrix: insights into evolutionary biology of fungal viruses 査読

    Juan Manuel Arjona-Lopez, Paul Telengech, Atif Jamal, Sakae Hisano, Hideki Kondo, Mery Dafny Yelin, Isabel Arjona-Girona, Satoko Kanematsu, Carlos José Lopez-Herrera, Nobuhiro Suzuki

    Environmental Microbiology   20 ( 4 )   1464 - 1483   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Blackwell Publishing Ltd  

    To reveal mycovirus diversity, we conducted a search of as-yet-unexplored Mediterranean isolates of the phytopathogenic ascomycete Rosellinia necatrix for virus infections. Of seventy-nine, eleven fungal isolates tested RNA virus-positive, with many showing coinfections, indicating a virus incidence of 14%, which is slightly lower than that (approximately 20%) previously reported for extensive surveys of over 1000 Japanese R. necatrix isolates. All viral sequences were fully or partially characterized by Sanger and next-generation sequencing. These sequences appear to represent isolates of various new species spanning at least 6 established or previously proposed families such as Partiti-, Hypo-, Megabirna-, Yado-kari-, Fusagra- and Fusarividae, as well as a newly proposed family, Megatotiviridae. This observation greatly expands the diversity of R. necatrix viruses, because no hypo-, fusagra- or megatotiviruses were previously reported from R. necatrix. The sequence analyses showed a rare horizontal gene transfer event of the 2A-like protease domain between a dsRNA (phlegivirus) and a positive-sense, single-stranded RNA virus (hypovirus). Moreover, many of the newly detected viruses showed the closest relation to viruses reported from fungi other than R. necatrix, such as Fusarium spp., which are sympatric to R. necatrix. These combined results imply horizontal virus transfer between these soil-inhabitant fungi.

    DOI: 10.1111/1462-2920.14065

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  • ICTV Virus Taxonomy Profile: Rhabdoviridae. 国際誌

    Peter J Walker, Kim R Blasdell, Charles H Calisher, Ralf G Dietzgen, Hideki Kondo, Gael Kurath, Ben Longdon, David M Stone, Robert B Tesh, Noël Tordo, Nikos Vasilakis, Anna E Whitfield, Ictv Report Consortium

    The Journal of general virology   99 ( 4 )   447 - 448   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The family Rhabdoviridae comprises viruses with negative-sense (-) single-stranded RNA genomes of 10.8-16.1 kb. Virions are typically enveloped with bullet-shaped or bacilliform morphology but can also be non-enveloped filaments. Rhabdoviruses infect plants and animals including mammals, birds, reptiles and fish, as well as arthropods which serve as single hosts or act as biological vectors for transmission to animals or plants. Rhabdoviruses include important pathogens of humans, livestock, fish and agricultural crops. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of Rhabdoviridae, which is available at www.ictv.global/report/rhabdoviridae.

    DOI: 10.1099/jgv.0.001020

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  • Phytopathogenic fungus hosts a plant virus: A naturally occurring cross-kingdom viral infection 査読

    Ida Bagus Andika, Shuang Wei, Chunmei Cao, Lakha Salaipeth, Hideki Kondo, Liying Sun

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 46 )   12267 - 12272   2017年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    The transmission of viral infections between plant and fungal hosts has been suspected to occur, based on phylogenetic and other findings, but has not been directly observed in nature. Here, we report the discovery of a natural infection of the phytopathogenic fungus Rhizoctonia solani by a plant virus, cucumber mosaic virus (CMV). The CMV-infected R. solani strain was obtained from a potato plant growing in Inner Mongolia Province of China, and CMV infection was stable when this fungal strain was cultured in the laboratory. CMV was horizontally transmitted through hyphal anastomosis but not vertically through basidiospores. By inoculation via protoplast transfection with virions, a reference isolate of CMV replicated in R. solani and another phytopathogenic fungus, suggesting that some fungi can serve as alternative hosts to CMV. Importantly, in fungal inoculation experiments under laboratory conditions, R. solani could acquire CMV from an infected plant, as well as transmit the virus to an uninfected plant. This study presents evidence of the transfer of a virus between plant and fungus, and it further expands our understanding of plant-fungus interactions and the spread of plant viruses.

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  • A possible occurrence of genome reassortment among bipartite rhabdoviruses 査読

    Hideki Kondo, Keisuke Hirota, Kazuyuki Maruyama, Ida Bagus An'dika, Nobuhiro Suzuki

    VIROLOGY   508   18 - 25   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Orchid fleck virus (OFV) represents a rhabdovirus with a unique bipartite genome. OFV genetic diversity at the whole genome level has not been described. Using the partial genome sequence of RNA1, we have determined that several OFV isolates derived from orchids in Japan belong to two genetically distant subgroups: subgroup I, the members of which are distributed worldwide but previously not known in Asia, and subgroup II, which is commonly distributed in Japan. However, complete genome sequence analysis of a novel Japanese subgroup I isolate revealed that although its RNA1 sequence differs considerably from those of subgroup II isolates, its RNA2 sequence is almost identical to them. Based on phylogenetic and recombination analyses, the genome reassortment events were predicted to occur between OFV subgroups including other unseen strains. Our data show that genome reassortment contributes to the genetic diversities of the bipartite rhabdoviruses and its occurrence may be geographically constrained.

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  • Taxonomy of the order Mononegavirales: update 2017. 国際誌

    Gaya K Amarasinghe, Yīmíng Bào, Christopher F Basler, Sina Bavari, Martin Beer, Nicolás Bejerman, Kim R Blasdell, Alisa Bochnowski, Thomas Briese, Alexander Bukreyev, Charles H Calisher, Kartik Chandran, Peter L Collins, Ralf G Dietzgen, Olga Dolnik, Ralf Dürrwald, John M Dye, Andrew J Easton, Hideki Ebihara, Qi Fang, Pierre Formenty, Ron A M Fouchier, Elodie Ghedin, Robert M Harding, Roger Hewson, Colleen M Higgins, Jian Hong, Masayuki Horie, Anthony P James, Dàohóng Jiāng, Gary P Kobinger, Hideki Kondo, Gael Kurath, Robert A Lamb, Benhur Lee, Eric M Leroy, Ming Li, Andrea Maisner, Elke Mühlberger, Sergey V Netesov, Norbert Nowotny, Jean L Patterson, Susan L Payne, Janusz T Paweska, Michael N Pearson, Rick E Randall, Peter A Revill, Bertus K Rima, Paul Rota, Dennis Rubbenstroth, Martin Schwemmle, Sophie J Smither, Qisheng Song, David M Stone, Ayato Takada, Calogero Terregino, Robert B Tesh, Keizo Tomonaga, Noël Tordo, Jonathan S Towner, Nikos Vasilakis, Viktor E Volchkov, Victoria Wahl-Jensen, Peter J Walker, Beibei Wang, David Wang, Fei Wang, Lin-Fa Wang, John H Werren, Anna E Whitfield, Zhichao Yan, Gongyin Ye, Jens H Kuhn

    Archives of virology   162 ( 8 )   2493 - 2504   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In 2017, the order Mononegavirales was expanded by the inclusion of a total of 69 novel species. Five new rhabdovirus genera and one new nyamivirus genus were established to harbor 41 of these species, whereas the remaining new species were assigned to already established genera. Furthermore, non-Latinized binomial species names replaced all paramyxovirus and pneumovirus species names, thereby accomplishing application of binomial species names throughout the entire order. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).

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  • Possibility and Challenges of Conversion of Current Virus Species Names to Linnaean Binomials. 国際誌

    Thomas S Postler, Anna N Clawson, Gaya K Amarasinghe, Christopher F Basler, Sbina Bavari, Mária Benko, Kim R Blasdell, Thomas Briese, Michael J Buchmeier, Alexander Bukreyev, Charles H Calisher, Kartik Chandran, Rémi Charrel, Christopher S Clegg, Peter L Collins, De La Torre Juan Carlos, Joseph L Derisi, Ralf G Dietzgen, Olga Dolnik, Ralf Dürrwald, John M Dye, Andrew J Easton, Sébastian Emonet, Pierre Formenty, Ron A M Fouchier, Elodie Ghedin, Jean-Paul Gonzalez, Balázs Harrach, Roger Hewson, Masayuki Horie, Dàohóng Jiang, Gary Kobinger, Hideki Kondo, Andrew M Kropinski, Mart Krupovic, Gael Kurath, Robert A Lamb, Eric M Leroy, Igor S Lukashevich, Andrea Maisner, Arcady R Mushegian, Sergey V Netesov, Norbert Nowotny, Jean L Patterson, Susan L Payne, Janusz T PaWeska, Clarence J Peters, Sheli R Radoshitzky, Bertus K Rima, Victor Romanowski, Dennis Rubbenstroth, Sead Sabanadzovic, Hélène Sanfaçon, Maria S Salvato, Martin Schwemmle, Sophie J Smither, Mark D Stenglein, David M Stone, Ayato Takada, Robert B Tesh, Keizo Tomonaga, Noël Tordo, Jonathan S Towner, Nikos Vasilakis, Viktor E Volchkov, Victoria Wahl-Jensen, Peter J Walker, Lin-Fa Wang, Arvind Varsani, Anna E Whitfield, F Murilo Zerbini, Jens H Kuhn

    Systematic biology   66 ( 3 )   463 - 473   2017年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Botanical, mycological, zoological, and prokaryotic species names follow the Linnaean format, consisting of an italicized Latinized binomen with a capitalized genus name and a lower case species epithet (e.g., Homo sapiens). Virus species names, however, do not follow a uniform format, and, even when binomial, are not Linnaean in style. In this thought exercise, we attempted to convert all currently official names of species included in the virus family Arenaviridae and the virus order Mononegavirales to Linnaean binomials, and to identify and address associated challenges and concerns. Surprisingly, this endeavor was not as complicated or time-consuming as even the authors of this article expected when conceiving the experiment. [Arenaviridae; binomials; ICTV; International Committee on Taxonomy of Viruses; Mononegavirales; virus nomenclature; virus taxonomy.].

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  • SAGA complex mediates the transcriptional up-regulation of antiviral RNA silencing 査読

    Ida Bagus Andika, Atif Jamal, Hideki Kondo, Nobuhiro Suzuki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 17 )   E3499 - E3506   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Pathogen recognition and transcriptional activation of defense-related genes are crucial steps in cellular defense responses. RNA silencing (RNAi) functions as an antiviral defense in eukaryotic organisms. Several RNAi-related genes are known to be transcriptionally up-regulated upon virus infection in some host organisms, but little is known about their induction mechanism. A phytopathogenic ascomycete, Cryphonectria parasitica (chestnut blight fungus), provides a particularly advantageous system to study RNAi activation, because its infection by certain RNA viruses induces the transcription of dicer-like 2 (dcl2) and argonaute-like 2 (agl2), two major RNAi players. To identify cellular factors governing activation of antiviral RNAi in C. parasitica, we developed a screening protocol entailing multiple transformations of the fungus with cDNA of a hypovirus mutant lacking the RNAi suppressor (CHV1-Delta p69), a reporter construct with a GFP gene driven by the dcl2 promoter, and a random mutagenic construct. Screening for GFP-negative colonies allowed the identification of sgf73, a component of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, a well-known transcriptional coactivator. Knockout of other SAGA components showed that the histone acetyltransferase module regulates transcriptional induction of dcl2 and agl2, whereas histone deubiquitinase mediates regulation of agl2 but not dcl2. Interestingly, full-scale induction of agl2 and dcl2 by CHV1 Delta p69 required both DCL2 and AGL2, whereas that by another RNA virus, mycoreovirus 1, required only DCL2, uncovering additional roles for DCL2 and AGL2 in viral recognition and/or RNAi activation. Overall, these results provide insight into the mechanism of RNAi activation.

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  • The family Rhabdoviridae: mono- and bipartite negative-sense RNA viruses with diverse genome organization and common evolutionary origins. 国際誌

    Ralf G Dietzgen, Hideki Kondo, Michael M Goodin, Gael Kurath, Nikos Vasilakis

    Virus research   227   158 - 170   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The family Rhabdoviridae consists of mostly enveloped, bullet-shaped or bacilliform viruses with a negative-sense, single-stranded RNA genome that infect vertebrates, invertebrates or plants. This ecological diversity is reflected by the diversity and complexity of their genomes. Five canonical structural protein genes are conserved in all rhabdoviruses, but may be overprinted, overlapped or interspersed with several novel and diverse accessory genes. This review gives an overview of the characteristics and diversity of rhabdoviruses, their taxonomic classification, replication mechanism, properties of classical rhabdoviruses such as rabies virus and rhabdoviruses with complex genomes, rhabdoviruses infecting aquatic species, and plant rhabdoviruses with both mono- and bipartite genomes.

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  • Barley Yellow Mosaic Virus VPg Is the Determinant Protein for Breaking eIF4E-Mediated Recessive Resistance in Barley Plants 査読

    Huangai Li, Hideki Kondo, Thomas Kuehne, Yukio Shirako

    FRONTIERS IN PLANT SCIENCE   7   1449   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS MEDIA SA  

    In this study, we investigated the barley yellow mosaic virus (BaYMV, genus Bymovirus) factor(s) responsible for breaking eIF4E-mediated recessive resistance genes (rym4/5/6) in barley. Genome mapping analysis using chimeric infectious cDNA clones between rym5-breaking (JT10) and rym5-non-breaking (JK05) isolates indicated that genome-linked viral protein (VPg) is the determinant protein for breaking the rym5 resistance. Likewise, VPg is also responsible for overcoming the resistances of rym4 and rym6 alleles. Mutational analysis identified that amino acids Ser-118, Thr-120, and His-142 in JT10 VPg are the most critical residues for overcoming rym5 resistance in protoplasts. Moreover, the rym5-non-breaking JK05 could accumulate in the rym5 protoplasts when elF4E derived from a susceptible barley cultivar was expressed from the viral genome. Thus, the compatibility between VPg and host eIF4E determines the ability of BaYMV to infect barley plants.

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  • Taxonomy of the order Mononegavirales: update 2016. 国際誌

    Claudio L Afonso, Gaya K Amarasinghe, Krisztián Bányai, Yīmíng Bào, Christopher F Basler, Sina Bavari, Nicolás Bejerman, Kim R Blasdell, François-Xavier Briand, Thomas Briese, Alexander Bukreyev, Charles H Calisher, Kartik Chandran, Jiāsēn Chéng, Anna N Clawson, Peter L Collins, Ralf G Dietzgen, Olga Dolnik, Leslie L Domier, Ralf Dürrwald, John M Dye, Andrew J Easton, Hideki Ebihara, Szilvia L Farkas, Juliana Freitas-Astúa, Pierre Formenty, Ron A M Fouchier, Yànpíng Fù, Elodie Ghedin, Michael M Goodin, Roger Hewson, Masayuki Horie, Timothy H Hyndman, Dàohóng Jiāng, Elliot W Kitajima, Gary P Kobinger, Hideki Kondo, Gael Kurath, Robert A Lamb, Sergio Lenardon, Eric M Leroy, Ci-Xiu Li, Xian-Dan Lin, Lìjiāng Liú, Ben Longdon, Szilvia Marton, Andrea Maisner, Elke Mühlberger, Sergey V Netesov, Norbert Nowotny, Jean L Patterson, Susan L Payne, Janusz T Paweska, Rick E Randall, Bertus K Rima, Paul Rota, Dennis Rubbenstroth, Martin Schwemmle, Mang Shi, Sophie J Smither, Mark D Stenglein, David M Stone, Ayato Takada, Calogero Terregino, Robert B Tesh, Jun-Hua Tian, Keizo Tomonaga, Noël Tordo, Jonathan S Towner, Nikos Vasilakis, Martin Verbeek, Viktor E Volchkov, Victoria Wahl-Jensen, John A Walsh, Peter J Walker, David Wang, Lin-Fa Wang, Thierry Wetzel, Anna E Whitfield, Ji Tāo Xiè, Kwok-Yung Yuen, Yong-Zhen Zhang, Jens H Kuhn

    Archives of virology   161 ( 8 )   2351 - 60   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In 2016, the order Mononegavirales was emended through the addition of two new families (Mymonaviridae and Sunviridae), the elevation of the paramyxoviral subfamily Pneumovirinae to family status (Pneumoviridae), the addition of five free-floating genera (Anphevirus, Arlivirus, Chengtivirus, Crustavirus, and Wastrivirus), and several other changes at the genus and species levels. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).

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  • VIPP1 Has a Disordered C-Terminal Tail Necessary for Protecting Photosynthetic Membranes against Stress 査読

    Lingang Zhang, Hideki Kondo, Hironari Kamikubo, Mikio Kataoka, Wataru Sakamoto

    PLANT PHYSIOLOGY   171 ( 3 )   1983 - 1995   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Integrity of biomembranes is vital to living organisms. In bacteria, PspA is considered to act as repairing damaged membrane by forming large supercomplexes in Arabidopsis (Arabidopsis thaliana). Vulnerable to oxidative stress, photosynthetic organisms also contain a PspA ortholog called VIPP1, which has an additional C-terminal tail (Vc). In this study, Vc was shown to coincide with an intrinsically disordered region, and the role of VIPP1 in membrane protection against stress was investigated. We visualized VIPP1 by fusing it to GFP (VIPP1-GFP that fully complemented lethal vipp1 mutations), and investigated its behavior in vivo with live imaging. The intrinsically disordered nature of Vc enabled VIPP1 to form what appeared to be functional particles along envelopes, whereas the deletion of Vc caused excessive association of the VIPP1 particles, preventing their active movement for membrane protection. Expression of VIPP1 lacking Vc complemented vipp1 mutation, but exhibited sensitivity to heat shock stress. Conversely, transgenic plants over-expressing VIPP1 showed enhanced tolerance against heat shock, suggesting that Vc negatively regulates VIPP1 particle association and acts in maintaining membrane integrity. Our data thus indicate that VIPP1 is involved in the maintenance of photosynthetic membranes. During evolution, chloroplasts have acquired enhanced tolerance against membrane stress by incorporating a disordered C-terminal tail into VIPP1.

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  • A novel betapartitivirus RnPV6 from Rosellinia necatrix tolerates host RNA silencing but is interfered by its defective RNAs 査読

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    VIRUS RESEARCH   219   62 - 72   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The family Partitiviridae comprises of five genera with bi-segmented dsRNA genomes that accommodate members infecting plants, fungi or protists. All partitiviruses with only a few exceptions cause asymptomatic infections. We report the characterization of a novel betapartitivirus termed Rosellinia necatrix partitivirus 6 (RnPV6) from a field isolate of a plant pathogenic fungus, white root rot fungus. RnPV6 has typical partitivirus features: dsRNA1 and dsRNA2 are 2462 and 2499 bps in length encoding RNA dependent RNA polymerase and capsid protein. Purified particles are spherical with a diameter of 30 nm. Taking advantage of infectivity as virions, RnPV6 was introduced into a model filamentous fungal host, chestnut blight fungus to investigate virus/host interactions. Unlike other partitiviruses tested previously, RnPV6 induced profound phenotypic alterations with symptoms characterized by a reduced growth rate and enhanced pigmentation and was tolerant to host RNA silencing. In addition, a variety of defective RNAs derived from dsRNA1 appear after virion transfection. These sub-viral RNAs were shown to interfere with RnPV6 replication, at least for that of cognate segment dsRNA1. Presence of these sub-viral elements resulted in reduced symptom expression by RnPV6, suggesting their nature as defective-interfering RNAs. The features of RnPV6 are similar to but distinct from those of a previously reported alphapartitivirus, Rosellinia necatrix partitivirus 2 that is susceptible to RNA silencing. (C) 2015 Elsevier B.V. All rights reserved.

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  • Amyloplast Membrane Protein SUBSTANDARD STARCH GRAIN6 Controls Starch Grain Size in Rice Endosperm 査読

    Ryo Matsushima, Masahiko Maekawa, Miyako Kusano, Katsura Tomita, Hideki Kondo, Hideki Nishimura, Naoko Crofts, Naoko Fujita, Wataru Sakamoto

    PLANT PHYSIOLOGY   170 ( 3 )   1445 - 1459   2016年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Starch is a biologically and commercially important polymer of glucose. Starch is organized into starch grains (SGs) inside amyloplasts. The SG size differs depending on the plant species and is one of the most important factors for industrial applications of starch. There is limited information on genetic factors regulating SG sizes. In this study, we report the rice (Oryza sativa) mutant substandard starch grain6 (ssg6), which develops enlarged SGs in endosperm. Enlarged SGs are observed starting at 3 d after flowering. During endosperm development, a number of smaller SGs appear and coexist with enlarged SGs in the same cells. The ssg6 mutation also affects SG morphologies in pollen. The SSG6 gene was identified by map-based cloning and microarray analysis. SSG6 encodes a protein homologous to aminotransferase. SSG6 differs from other rice homologs in that it has a transmembrane domain. SSG6-green fluorescent protein is localized in the amyloplast membrane surrounding SGs in rice endosperm, pollen, and pericarp. The results of this study suggest that SSG6 is a novel protein that controls SG size. SSG6 will be a useful molecular tool for future starch breeding and applications.

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  • Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi 査読

    Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki

    Virus Research   213   353 - 364   2016年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier B.V.  

    The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. Powdery mildew fungi are important and widely studied obligate phytopathogenic agents, but there has been no report on mycoviruses infecting these fungi. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least ten totivirus (genus Totivirus)-like sequences, termed red clover powdery mildew-associated totiviruses (RPaTVs). The majority of these sequences shared moderate amino acid sequence identity with each other (&lt
    44%) and with other known totiviruses (&lt
    59%). Nine of these identified sequences (RPaTV1a, 1b and 2-8) resembled the genome of the prototype totivirus, Saccharomyces cerevisiae virus-L-A (ScV-L-A) in that they contained two overlapping open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA dependent RNA polymerase (RdRp), while one sequence (RPaTV9) showed similarity to another totivirus, Ustilago maydis virus H1 (UmV-H1) that encodes a single polyprotein (CP-RdRp fusion). Similar to yeast totiviruses, each ScV-L-A-like RPaTV contains a -1 ribosomal frameshift site downstream of a predicted pseudoknot structure in the overlapping region of these ORFs, suggesting that the RdRp is translated as a CP-RdRp fusion. Moreover, several ScV-L-A-like sequences were also found by searches of the transcriptome shotgun assembly (TSA) libraries from rust fungi, plants and insects. Phylogenetic analyses show that nine ScV-L-A-like RPaTVs along with ScV-L-A-like sequences derived from TSA libraries are clustered with most established members of the genus Totivirus, while one RPaTV forms a new distinct clade with UmV-H1, possibly establishing an additional genus in the family. Taken together, our results indicate the presence of diverse, novel totiviruses in the powdery mildew fungus populations infecting red clover plants in the field.

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  • A capsidless ssRNA virus hosted by an unrelated dsRNA virus 査読

    Rui Zhang, Sakae Hisano, Akio Tani, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    NATURE MICROBIOLOGY   1 ( 1 )   15001   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Viruses typically encode the capsid that encases their genome, while satellite viruses do not encode a replicase and depend on a helper virus for their replication(1). Here, we report interplay between two RNA viruses, yado-nushi virus 1 (YnV1) and yado-kari virus 1 (YkV1), in a phytopathogenic fungus, Rosellinia necatrix(2). YkV1 has a close phylogenetic affinity to positive-sense, single-stranded (+)ssRNA viruses such as animal caliciviruses(3), while YnV1 has an undivided double-stranded (ds) RNA genome with a resemblance to fungal toti-viruses(4). Virion transfection and infectious full-length cDNA transformation has shown that YkV1 depends on YnV1 for viability, although it probably encodes functional RNA-dependent RNA polymerase (RdRp). Immunological and molecular analyses have revealed trans-encapsidation of not only YkV1 RNA but also RdRp by the capsid protein of the other virus (YnV1), and enhancement of YnV1 accumulation by YkV1. This study demonstrates interplay in which the capsidless (+) ssRNA virus (YkV1), hijacks the capsid protein of the dsRNA virus (YnV1), and replicates as if it were a dsRNA virus.

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  • IDENTIFICATION OF GENOME RECOMBINATION AMONG APPLE STEM PITTING VIRUS ISOLATES 査読

    Z. Li, H. Kondo, I. B. Andika, P. Liu, L. Sun, Y. Wu

    JOURNAL OF PLANT PATHOLOGY   98 ( 3 )   595 - 601   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:EDIZIONI ETS  

    We have determined the complete genome sequence of an apple stem pitting virus isolate (ASPV, genus Foveavirus) from China, providing a new genome sequence of an ASPV isolate from apple trees. Nucleotide identities and phylogenetic relationships among this and another eleven complete sequences of ASPV isolates largely differed according to the coding regions of the genome analysed, suggesting the possible occurrence of recombination. Using recombination-detection programs, multiple recombination events throughout the ASPV genome were predicted to occur among isolates, regardless of their host species. Our analyses suggest that pervasive genome recombination drives the evolution and genetic diversity of ASPV.

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  • Interplays between Soil-Borne Plant Viruses and RNA Silencing-Mediated Antiviral Defense in Roots. 国際誌

    Ida Bagus Andika, Hideki Kondo, Liying Sun

    Frontiers in microbiology   7   1458 - 1458   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Although the majority of plant viruses are transmitted by arthropod vectors and invade the host plants through the aerial parts, there is a considerable number of plant viruses that infect roots via soil-inhabiting vectors such as plasmodiophorids, chytrids, and nematodes. These soil-borne viruses belong to diverse families, and many of them cause serious diseases in major crop plants. Thus, roots are important organs for the life cycle of many viruses. Compared to shoots, roots have a distinct metabolism and particular physiological characteristics due to the differences in development, cell composition, gene expression patterns, and surrounding environmental conditions. RNA silencing is an important innate defense mechanism to combat virus infection in plants, but the specific information on the activities and molecular mechanism of RNA silencing-mediated viral defense in root tissue is still limited. In this review, we summarize and discuss the current knowledge regarding RNA silencing aspects of the interactions between soil-borne viruses and host plants. Overall, research evidence suggests that soil-borne viruses have evolved to adapt to the distinct mechanism of antiviral RNA silencing in roots.

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  • Cymbidium chlorotic mosaic virus, a new sobemovirus isolated from a spring orchid (Cymbidium goeringii) in Japan 査読

    Hideki Kondo, Shogo Takemoto, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY   160 ( 8 )   2099 - 2104   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    Cymbidium chlorotic mosaic virus (CyCMV), isolated from a spring orchid (Cymbidium goeringii), was characterized molecularly. CyCMV isometric virions comprise a single, positive-strand RNA genome of 4,083 nucleotides and 30-kDa coat protein. The virus genome contains five overlapping open reading frames with a genomic organization similar to that of sobemoviruses. BLAST searches and phylogenetic analysis revealed that CyCMV is most closely related to papaya lethal yellowing virus, a proposed dicot-infecting sobemovirus (58.8 % nucleotide sequence identity), but has a relatively distant relationship to monocot-infecting sobemoviruses, with only modest sequence identities. This suggests that CyCMV is a new monocot-infecting member of the floating genus Sobemovirus.

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  • Differential contributions of plant Dicer-like proteins to antiviral defences against potato virus X in leaves and roots 査読

    Ida Bagus Andika, Kazuyuki Maruyama, Liying Sun, Hideki Kondo, Tetsuo Tamada, Nobuhiro Suzuki

    PLANT JOURNAL   81 ( 5 )   781 - 793   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Members of the plant Dicer-like (DCL) protein family are the critical components of the RNA-silencing pathway that mediates innate antiviral defence. The distinct antiviral role of each individual DCL protein has been established with mostly based on observations of aerial parts of plants. Thus, although the roots are closely associated with the life cycle of many plant viruses, little is known about the antiviral activities of DCL proteins in roots. We observed that antiviral silencing strongly inhibits potato virus X (PVX) replication in roots of some susceptible Solanaceae species. Silencing of the DCL4 homolog in Nicotiana benthamiana partially elevated PVX replication levels in roots. In Arabidopsis thaliana, which was originally considered a non-host plant of PVX, high levels of PVX accumulation in inoculated leaves were achieved by inactivation of DCL4, while in the upper leaves and roots, it required the additional inactivation of DCL2. In transgenic A.thaliana carrying the PVX amplicon with a green fluorescent protein (GFP) gene insertion in the chromosome (AMP243 line), absence of DCL4 enabled high levels of PVX-GFP accumulation in various aerial organs but not in the roots, suggesting that DCL4 is critical for intracellular antiviral silencing in shoots but not in roots, where it can be functionally compensated by other DCL proteins. Together, the high level of functional redundancies among DCL proteins may contribute to the potent antiviral activities against PVX replication in roots.
    Significance StatementThis study demonstrates the differential contributions of DCL proteins between leaves and roots.

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  • Different Dicer-like protein components required for intracellular and systemic antiviral silencing in Arabidopsis thaliana. 国際誌

    Ida Bagus Andika, Kazuyuki Maruyama, Liying Sun, Hideki Kondo, Tetsuo Tamada, Nobuhiro Suzuki

    Plant signaling & behavior   10 ( 8 )   e1039214   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Eukaryotes employ RNA silencing as an innate defense system against invading viruses. Dicer proteins play the most crucial role in initiating this antiviral pathway as they recognize and process incoming viral nucleic acids into small interfering RNAs. Generally, 2 successive infection stages constitute viral infection in plants. First, the virus multiplies in initially infected cells or organs after viral transmission and then the virus subsequently spreads systemically through the vasculature to distal plant tissues or organs. Thus, antiviral silencing in plants must cope with both local and systemic invasion of viruses. In a recent study using 2 sets of different experiments, we clearly demonstrated the differential requirement for Dicer-like 4 (DCL4) and DCL2 proteins in the inhibition of intracellular and systemic infection by potato virus X in Arabidopsis thaliana. Taken together with the results of other studies, here we further discuss the functional specificity of DCL proteins in the antiviral silencing pathway.

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  • A novel single-stranded RNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix, with similarity to hypo-like viruses 査読

    Rui Zhang, Shengxue Liu, Sotaro Chiba, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    FRONTIERS IN MICROBIOLOGY   5   360   2014年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS RESEARCH FOUNDATION  

    Here we report a biological and molecular characterization of a novel positive-sense RNA virus isolated from a field isolate (NW10) of a filamentous phytopathogenic fungus, the white root rot fungus that is designated as Rosellinia necatrix fusarivirus 1 (RnFV1). A recently developed technology using zinc ions allowed us to transfer RnFV1 to two mycelially incompatible Rosellinia necatrix strains. A biological comparison of the virus-free and -recipient isogenic fungal strains suggested that RnFV1 infects latently and thus has no potential as a virocontrol agent. The virus has an undivided positive-sense RNA genome of 6286 nucleotides excluding a poly (A) tail. The genome possesses two non-overlapping open reading frames (ORFs): a large ORF1 that encodes polypeptides with RNA replication functions and a smaller ORF2 that encodes polypeptides of unknown function. A lack of coat protein genes was suggested by the failure of virus particles from infected mycelia. No evidence was obtained by Northern analysis or classical 5'-RACE for the presence of subgenomic RNA for the downstream ORE Sequence similarities were found in amino-acid sequence between RnFV1 putative proteins and counterparts of a previously reported mycovirus, Fusarium graminearum virus 1 (FgV1). Interestingly, several related sequences were detected by BLAST searches of independent transcriptome assembly databases one of which probably represents an entire virus genome. Phylogenetic analysis based on the conserved RNA-dependent RNA polymerase showed that RnFV1, FgV1, and these similar sequences are grouped in a cluster distinct from distantly related hypoviruses. It is proposed that a new taxonomic family termed Fusariviridae be created to include RnFV1 and FgV1.

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  • Transcriptional mapping of the messenger and leader RNAs of orchid fleck virus, a bisegmented negative-strand RNA virus 査読

    Hideki Kondo, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    VIROLOGY   452   166 - 174   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The transcriptional strategy of orchid fleck virus (OFV), which has a two-segmented negative-strand RNA genome and resembles plant nucleorhabdoviruses, remains unexplored. In this study, the transcripts of six genes encoded by OFV RNA1 and RNA2 in the poly(A)-enriched RNA fraction from infected plants were molecularly characterized. All of the OFV mRNAs were initiated at a start sequence 3'-UU-5' with one to three non-viral adenine nucleotides which were added at the 5' end of each mRNA, whereas their 3' termini ended with a 5'-AUUUAAA(U/G)AAAA(A)n-3' sequence. We also identified the presence of polyadenylated short transcripts derived from the 3'-terminal leader regions of both genomic and antigenomic strands, providing the first example of plus- and minus-strand leader RNAs in a segmented minus-strand RNA virus. The similarity in the transcriptional strategy between this bipartite OFV and monopartite rhabdoviruses, especially nucleorhabdoviruses (family Rhabdoviridae) is additional support for their close relationship. (C) 2014 Elsevier Inc. All rights reserved.

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  • Dichorhavirus: a proposed new genus for Brevipalpus mite-transmitted, nuclear, bacilliform, bipartite, negative-strand RNA plant viruses. 国際誌

    Ralf G Dietzgen, Jens H Kuhn, Anna N Clawson, Juliana Freitas-Astúa, Michael M Goodin, Elliott W Kitajima, Hideki Kondo, Thierry Wetzel, Anna E Whitfield

    Archives of virology   159 ( 3 )   607 - 19   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Orchid fleck virus (OFV) is an unassigned negative-sense, single-stranded (-)ssRNA plant virus that was previously suggested to be included in the family Rhabdoviridae, order Mononegavirales. Although OFV shares some biological characteristics, including nuclear cytopathological effects, gene order, and sequence similarities, with nucleorhabdoviruses, its taxonomic status is unclear because unlike all mononegaviruses, OFV has a segmented genome and its particles are not enveloped. This article analyses the available biological, physico-chemical, and nucleotide sequence evidence that seems to indicate that OFV and several other Brevipalpus mite-transmitted short bacilliform (-)ssRNA viruses are likely related and may be classified taxonomically in novel species in a new free-floating genus Dichorhavirus.

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  • Amyloplast-Localized SUBSTANDARD STARCH GRAIN4 Protein Influences the Size of Starch Grains in Rice Endosperm 査読

    Ryo Matsushima, Masahiko Maekawa, Miyako Kusano, Hideki Kondo, Naoko Fujita, Yasushi Kawagoe, Wataru Sakamoto

    PLANT PHYSIOLOGY   164 ( 2 )   623 - 636   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Starch is a biologically and commercially important polymer of glucose and is synthesized to form starch grains (SGs) inside amyloplasts. Cereal endosperm accumulates starch to levels that are more than 90% of the total weight, and most of the intracellular space is occupied by SGs. The size of SGs differs depending on the plant species and is one of the most important factors for industrial applications of starch. However, the molecular machinery that regulates the size of SGs is unknown. In this study, we report a novel rice (Oryza sativa) mutant called substandard starch grain4 (ssg4) that develops enlarged SGs in the endosperm. Enlargement of SGs in ssg4 was also observed in other starch-accumulating tissues such as pollen grains, root caps, and young pericarps. The SSG4 gene was identified by map-based cloning. SSG4 encodes a protein that contains 2,135 amino acid residues and an amino-terminal amyloplast-targeted sequence. SSG4 contains a domain of unknown function490 that is conserved from bacteria to higher plants. Domain of unknown function490-containing proteins with lengths greater than 2,000 amino acid residues are predominant in photosynthetic organisms such as cyanobacteria and higher plants but are minor in proteobacteria. The results of this study suggest that SSG4 is a novel protein that influences the size of SGs. SSG4 will be a useful molecular tool for future starch breeding and biotechnology.

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  • Complete genome sequence of Habenaria mosaic virus, a new potyvirus infecting a terrestrial orchid (Habenaria radiata) in Japan 査読

    Hideki Kondo, Takanori Maeda, I. Wayan Gara, Sotaro Chiba, Kazuyuki Maruyama, Tetsuo Tamada, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY   159 ( 1 )   163 - 166   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    The complete genomic sequence of Habenaria mosaic virus (HaMV), which infects terrestrial orchids (Habenaria radiata), has been determined. The genome is composed of 9,499 nucleotides excluding the 3'-terminal poly(A) tail, encoding a large polyprotein of 3,054 amino acids with the genomic features typical of a potyvirus. Putative proteolytic cleavage sites were identified by sequence comparison to those of known potyviruses. The HaMV polyprotein showed 58 % amino acid sequence identity to that encoded by the most closely related potyvirus, tobacco vein banding mosaic virus. Phylogenetic analysis of the polyprotein amino acid sequence and its coding sequences confirmed that HaMV formed a cluster with the chilli veinal mottle virus group, most of which infect solanaceous plants. These results suggest that HaMV is a distinct member of the genus Potyvirus.

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  • Nucleo-cytoplasmic shuttling of VPg encoded by Wheat yellow mosaic virus requires association with the coat protein 査読

    Liying Sun, Bian Jing, Ida Bagus Andika, Yingchun Hu, Bingjian Sun, Rong Xiang, Hideki Kondo, Jianping Chen

    JOURNAL OF GENERAL VIROLOGY   94 ( Pt 12 )   2790 - 2802   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    VPg (virus protein, genome-linked) is a multifunctional protein that plays important roles in viral multiplication in the cytoplasm. However, a number of VPgs encoded by plant viruses target the nucleus and this appears to be biologically significant. These VPgs may therefore be translocated between nuclear and cytoplasmic compartments during virus infection, but such nucleo-cytoplasmic transport has not been demonstrated. We report that VPg encoded by Wheat yellow mosaic virus (WYMV, genus Bymovirus, family Potyviridae) accumulated in both the nucleus and cytoplasm of infected cells, but localized exclusively in the nucleus when expressed alone in plants. Computational analyses predicted the presence of a nuclear localization signal (NLS) and a nuclear export signal (NES) in WYMV VPg. Mutational analyses showed that both the N-terminal and the NLS domains of VPg contribute to the efficiency of nuclear targeting. In vitro and in planta assays indicated that VPg interacts with WYMV coat protein (CP) and proteinase 1 (P1) proteins. Observation of VPg fused to a fluorescent protein and subcellular fractionation experiments showed that VPg was translocated to the cytoplasm when co-expressed with CP, but not with P1. Mutations in the NES domain or treatment with leptomycin B prevented VPg translocation to the cytoplasm when co-expressed with CP. Our results suggest that association with CP facilitates the nuclear export of VPg during WYMV infection.

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  • Characterization of burdock mottle virus, a novel member of the genus Benyvirus, and the identification of benyvirus-related sequences in the plant and insect genomes 査読

    Hideki Kondo, Shuichi Hirano, Sotaro Chiba, Ida Bagus Andika, Makoto Hirai, Takanori Maeda, Tetsuo Tamada

    Virus Research   177 ( 1 )   75 - 86   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The complete nucleotide sequence of the burdock mottle virus (BdMoV) isolated from an edible burdock plant ( Arctium lappa) in Japan has been determined. BdMoV has a bipartite genome, whose organization is similar to RNA1 and RNA2 of benyviruses, beet necrotic yellow vein virus (BNYVV), beet soil-borne mosaic virus (BSBMV), and rice stripe necrosis virus (RSNV). BdMoV RNA1 (7038 nt) contains a single open reading frame (ORF) encoding a 249-kDa polypeptide that consists of methyl-transferase, helicase, papain-like protease, AlkB-like, and RNA-dependent RNA polymerase domains. The AlkB-like domain sequence is not present in the proteins encoded by other known benyviruses, but is found in replication-associated proteins of viruses mainly belonging to the families Alfaflexiviridae and Betaflexiviridae. BdMoV RNA2 (4315 nt) contains six ORFs that are similar to those of benyviruses: these are coat protein (CP), CP readthrough, triple gene block movement and cysteine-rich proteins. Phylogenetic analyses showed that BdMoV is more closely related to BNYVV and BSBMV than to RSNV. Database searches showed that benyvirus replicase-related sequences are present in the chromosomes of a chickpea plant ( Cicer arietinum) and a blood-sucking insect ( Rhodnius prolixus). Some other benyvirus-related sequences are found in the transcriptome shotgun libraries of a few species of plants and a bark beetle. Our results show that BdMoV is a distinct species of the genus Benyvirus and that ancestral and extant benyviruses may have infected or currently infect a wide range of hosts, including plants and insects. © 2013 Elsevier B.V.

    DOI: 10.1016/j.virusres.2013.07.015

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  • Nyamiviridae: proposal for a new family in the order Mononegavirales. 国際誌

    Jens H Kuhn, Sadia Bekal, Yíngyún Caì, Anna N Clawson, Leslie L Domier, Marieke Herrel, Peter B Jahrling, Hideki Kondo, Kris N Lambert, Kathie A Mihindukulasuriya, Norbert Nowotny, Sheli R Radoshitzky, Urs Schneider, Peter Staeheli, Nobuhiro Suzuki, Robert B Tesh, David Wang, Lin-Fa Wang, Ralf G Dietzgen

    Archives of virology   158 ( 10 )   2209 - 26   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nyamanini virus (NYMV) and Midway virus (MIDWV) are unclassified tick-borne agents that infect land birds and seabirds, respectively. The recent molecular characterization of both viruses confirmed their already known close serological relationship and revealed them to be nonsegmented, single- and negative-stranded RNA viruses that are clearly related to, but quite distinct from, members of the order Mononegavirales (bornaviruses, filoviruses, paramyxoviruses, and rhabdoviruses). A third agent, soybean cyst nematode virus 1 (SbCNV-1, previously named soybean cyst nematode nyavirus), was recently found to be an additional member of this new virus group. Here, we review the current knowledge about all three viruses and propose classifying them as members of a new mononegaviral family, Nyamiviridae.

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  • Biological and genetic diversity of plasmodiophorid-transmitted viruses and their vectors 査読

    Tetsuo Tamada, Hideki Kondo

    JOURNAL OF GENERAL PLANT PATHOLOGY   79 ( 5 )   307 - 320   2013年9月

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    記述言語:英語   出版者・発行元:SPRINGER JAPAN KK  

    About 20 species of viruses belonging to five genera, Benyvirus, Furovirus, Pecluvirus, Pomovirus and Bymovirus, are known to be transmitted by plasmodiophorids. These viruses have all positive-sense, single-stranded RNA genomes that consist of two to five RNA components. Three species of plasmodiophorids are recognized as vectors: Polymyxa graminis, P. betae, and Spongospora subterranea. The viruses can survive in soil within the long-lived resting spores of the vector. There are biological and genetic variations in both virus and vector species. Many of the viruses are causal agents of important diseases in major crops such as rice, wheat, barley, rye, sugar beet, potato, and groundnut. Control is dependent on the development of resistant cultivars. During the last half century, several virus diseases have rapidly spread worldwide. For six major virus diseases, we address their geographical distribution, diversity, and genetic resistance.

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  • Orchid fleck virus structural proteins N and P form intranuclear viroplasm like structures in the absence of viral infection 査読

    Hideki Kondo, Sotaro Chiba, Ida Bagus Andika, Kazuyuki Maruyama, Tetsuo Tamada, Nobuhiro Suzuki

    Journal of Virology   87 ( 13 )   7423 - 7434   2013年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Orchid fleck virus (OFV) has a unique two-segmented negative-sense RNA genome that resembles that of plant nucleorhabdoviruses. In infected plant cells, OFV and nucleorhabdoviruses induce an intranuclear electron-lucent viroplasm that is believed to be the site for virus replication. In this study, we investigated the molecular mechanism by which OFV viroplasms are produced in vivo. Among OFV-encoded proteins, the nucleocapsid protein (N) and the putative phosphoprotein (P) were present in nuclear fractions of OFV-infected Nicotiana benthamiana plants. Transient coexpression of N and P, in the absence of virus infection, was shown to be sufficient for formation of an intranuclear viroplasm-like structure in plant cells. When expressed independently as a fluorescent protein fusion product in uninfected plant cells, N protein accumulated throughout the cell, while P protein accumulated in the nucleus. However, the N protein, when coexpressed with P, was recruited to a subnuclear region to induce a large viroplasm-like focus. Deletion and substitution mutagenesis demonstrated that the P protein contains a nuclear localization signal (NLS). Artificial nuclear targeting of the N-protein mutant was insufficient for formation of viroplasm-like structures in the absence of P. A bimolecular fluorescence complementation assay confirmed interactions between the N and P proteins within subnuclear viroplasm-like foci and interactions of two of the N. benthamiana importin-α homologues with the P protein but not with the N protein. Taken together, our results suggest that viroplasm formation by OFV requires nuclear accumulation of both the N and P proteins, which is mediated by P-NLS, unlike nucleorhabdovirus viroplasm utilizing the NLS on protein N. © 2013, American Society for Microbiology.

    DOI: 10.1128/JVI.00270-13

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  • A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum 査読

    Shuangchao Wang, Hideki Kondo, Liang Liu, Lihua Guo, Dewen Qiu

    VIRUS RESEARCH   174 ( 1-2 )   69 - 77   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    A double-stranded (ds) RNA element, sized at approximately 13 kb pairs, was purified from a field isolate, HN10, of Fusariumgraminearum.The coding strand of the dsRNA was 13,023 nucleotides (nt) long (excluding the 3' poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORF A and ORF B). The 5' proximal ORF A of 531 nt encoded a protein of 176 amino acids (aa), and a BLAST search showed it to be similar to the putative papain-like protease domains encoded by Valsa ceratosperma hypovirus 1 (35% identity) and Cryphonectria hypovirus 4 (CHV4) (31% identity). The 3' proximal ORF B of 11,118 nt encoded a large polyprotein with three conserved domains, including papain-like protease, RNA-dependent RNA polymerase and RNA helicase domains. The polyprotein shared significant aa identities with CHV1 (32%) and CHV2 (32%). Both the genome organization and phylogenetic analysis suggested that the characterized RNA represented a novel hypovirus, designated "Fusarium graminearum hypovirus 1 (FgHV1)", which was closely related to CHV1 and CHV2 in the Hypoviridae family. Elimination of the virus resulted in no dramatic phenotypic alteration of the fungus. (C) 2013 Elsevier B.V. All rights reserved.

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  • A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia necatrix, Is Infectious as Particles and Targeted by RNA Silencing 査読

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   87 ( 12 )   6727 - 6738   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    A novel victorivirus, termed Rosellinia necatrix victorivirus 1 (RnVV1), was isolated from a plant pathogenic ascomycete, white root rot fungus Rosellinia necatrix, coinfected with a partitivirus. The virus was molecularly and biologically characterized using the natural and experimental hosts (chestnut blight fungus, Cryphonectria parasitica). RnVV1 was shown to have typical molecular victorivirus attributes, including a monopartite double-stranded RNA genome with two open reading frames (ORFs) encoding capsid protein (CP) and RNA-dependent RNA polymerase (RdRp), a UAAUG pentamer presumed to facilitate the coupled termination/reinitiation for translation of the two ORFs, a spherical particle structure similar to 40 nm in diameter, and moderate levels of CP and RdRp sequence identity (34 to 58%) to those of members of the genus Victorivirus within the family Totiviridae. A reproducible transfection system with purified RnVV1 virions was developed for the two distinct fungal hosts. Transfection assay with purified RnVV1 virions combined with virus elimination by hyphal tipping showed that the effects of RnVV1 on the phenotype of the natural host were negligible. Interestingly, comparison of the RNA silencing-competent (standard strain EP155) and -defective (Delta dcl-2) strains of C. parasitica infected with RnVV1 showed that RNA silencing acted against the virus to repress its replication, which was restored by coinfection with hypovirus or transgenic expression of an RNA silencing suppressor, hypovirus p29. Phenotypic changes were observed in the Delta dcl-2 strain but not in EP155. This is the first reported study on the host range expansion of a Totiviridae member that is targeted by RNA silencing.

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  • Identification of a movement protein of Mirafiori lettuce big-vein ophiovirus 査読

    Akihiro Hiraguri, Shoko Ueki, Hideki Kondo, Koji Nomiyama, Takumi Shimizu, Tamaki Ichiki-Uehara, Toshihiro Omura, Nobumitsu Sasaki, Hiroshi Nyunoya, Takahide Sasaya

    Journal of General Virology   94 ( 5 )   1145 - 1150   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Mirafiori lettuce big-vein virus (MiLBVV) is a member of the genus Ophiovirus, which is a segmented negative-stranded RNA virus. In microprojectile bombardment experiments to identify a movement protein (MP) gene of ophioviruses that can trans-complement intercellular movement of an MP-deficient heterologous virus, a plasmid containing an infectious clone of a tomato mosaic virus (ToMV) derivative expressing the GFP was co-bombarded with plasmids containing one of three genes from MiLBVV RNAs 1, 2 and 4 onto Nicotiana benthamiana. Intercellular movement of the movement-defective ToMV was restored by co-expression of the 55 kDa protein gene, but not with the two other genes. Transient expression in epidermal cells of N. benthamiana and onion showed that the 55 kDa protein with GFP was localized on the plasmodesmata. The 55 kDa protein encoded in the MiLBVV RNA2 can function as an MP of the virus. This report is the first to describe an ophiovirus MP. © 2013 SGM.

    DOI: 10.1099/vir.0.050005-0

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  • Identification of the amino acid residues and domains in the cysteine-rich protein of Chinese wheat mosaic virus that are important for RNA silencing suppression and subcellular localization 査読

    Liying Sun, Ida Bagus Andika, Hideki Kondo, Jianping Chen

    Molecular Plant Pathology   14 ( 3 )   265 - 278   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cysteine-rich proteins (CRPs) encoded by some plant viruses in diverse genera function as RNA silencing suppressors. Within the N-terminal portion of CRPs encoded by furoviruses, there are six conserved cysteine residues and a Cys-Gly-X-X-His motif (Cys, cysteine
    Gly, glycine
    His, histidine
    X, any amino acid residue) with unknown function. The central domains contain coiled-coil heptad amino acid repeats that usually mediate protein dimerization. Here, we present evidence that the conserved cysteine residues and Cys-Gly-X-X-His motif in the CRP of Chinese wheat mosaic virus (CWMV) are critical for protein stability and silencing suppression activity. Mutation of a leucine residue in the third coiled-coil heptad impaired CWMV CRP activity for suppression of local silencing, but not for the promotion of cell-to-cell movement of Potato virus X (PVX). Inplanta and invitro analysis of wild-type and mutant proteins indicated that the ability of the CRP to self-interact was correlated with its suppression activity. Deletion of up to 40 amino acids at the C-terminus did not abolish suppression activity, but disrupted the association of CRP with endoplasmic reticulum (ER), and reduced its activity in the enhancement of PVX symptom severity. Interestingly, a short region in the C-terminal domain, predicted to form an amphipathic α-helical structure, was responsible for the association of CWMV CRP with ER. Overall, our results demonstrate that the N-terminal and central regions are the functional domains for suppression activity, whereas the C-terminal region primarily functions to target CWMV CRP to the ER. © 2012 The Authors. Molecular Plant Pathology © 2012 Bspp and Blackwell Publishing Ltd.

    DOI: 10.1111/mpp.12002

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  • Effects of Defective Interfering RNA on Symptom Induction by, and Replication of, a Novel Partitivirus from a Phytopathogenic Fungus, Rosellinia necatrix 査読

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   87 ( 4 )   2330 - 2341   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    A novel mycovirus termed Rosellinia necatrix partitivirus 2 (RnPV2), isolated from a phytopathogenic fungus, Rosellinina necatrix strain W57, was molecularly and biologically characterized in both natural and experimental host fungi. Three double-stranded RNA (dsRNA) segments, dsRNA1, dsRNA2, and defective interfering dsRNA1 (DI-dsRNA1), whose sizes were approximately 2.0, 1.8, and 1.7 kbp, respectively, were detected in W57. While the dsRNA2 sequence, encoding the coat protein, was reported previously, dsRNA1 and DI-dsRNA1 were shown to encode competent and defective (truncated) RNA-dependent RNA polymerase, respectively. Artificial introduction of RnPV2 into an RNA silencing-defective, Dicer-like 2 knockout mutant (Delta dcl-2) of a nonnatural host, Cryphonectria parasitica (chestnut blight fungus), resulted in successful infection by the DI-dsRNA1-carrying and -free RnPV2. The DI-dsRNA1-free RnPV2 strain was characterized by a higher ratio of accumulation of the intact dsRNA1 to dsRNA2, enhanced replication and severer symptom expression, compared with the DI-carrying strain. These findings confirmed the nature of DI-dsRNA1 as a DI-RNA. Both viral strains replicated to higher levels in a Delta dcl-2 mutant than in a wild-type C. parasitica fungal strain (EP155) and induced severe symptoms in the Delta dcl-2 mutant but subtle symptoms in EP155, indicating that the host RNA silencing targets the partitivirus. No obvious phenotypic effects of infection by either virus strain were detected in the natural host fungus. These combined results represent the first example of a partitivirus with DI-RNA that alters viral symptom induction in a host-dependent manner.

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  • Evidence for negative-strand RNA virus infection in fungi 査読

    Hideki Kondo, Sotaro Chiba, Kazuhiro Toyoda, Nobuhiro Suzuki

    Virology   435 ( 2 )   201 - 209   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Fungal viruses comprise two groups: a major group of five families with double-stranded RNA genomes and a minor group with positive-sense single-stranded (ss)RNA genomes. Although many fungal viruses have been identified, no negative-stranded (-)ssRNA mycoviruses have been reported. Here we present two lines of evidence suggesting the presence of (-)ssRNA viruses in filamentous fungi based on an exhaustive search using extant (-)ssRNA viruses as queries. This revealed (-)ssRNA virus L protein-like sequences in the genome of a phytopathogenic obligate ascomycete, Erysiphe pisi. A similar search for (-)ssRNA viruses in fungal transcriptome shotgun assembly libraries demonstrated that two independent libraries from Sclerotinia homoeocarpa, another phytopathogenic ascomycete, contained several sequences considered to correspond to the entire mononegavirus L gene and likely originating from an infecting (-)ssRNA virus. These results provide strong evidence for both ancient and extant (-)ssRNA virus infections in fungi. © 2012 Elsevier Inc.

    DOI: 10.1016/j.virol.2012.10.002

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  • Endoplasmic reticulum export and vesicle formation of the movement protein of Chinese wheat mosaic virus are regulated by two transmembrane domains and depend on the secretory pathway 査読

    Ida Bagus Andika, Shiling Zheng, Zilong Tan, Liying Sun, Hideki Kondo, Xueping Zhou, Jianping Chen

    VIROLOGY   435 ( 2 )   493 - 503   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The 37K protein of Chinese wheat mosaic virus (CWMV) belongs to the 30K superfamily of plant virus movement proteins. CWMV 37K trans-complemented the cell-to-cell spread of a movement-defective Potato virus X. CWMV 37K fused to enhanced green fluorescent protein localized to plasmodesmata and formed endoplasmic reticulum (ER)-derived vesicular and large aggregate structures. CWMV 37K has two putative N-terminal transmembrane domains (TMDs). Mutations disrupting TMD1 or TMD2 impaired 37K movement function; those mutants were unable to form ER-derived structures but instead accumulated in the ER. Treatment with Brefeldin A or overexpression of the dominant negative mutant of Sari retained 37K in the ER, indicating that ER export of 37K is dependent on the secretory pathway. Moreover, CWMV 37K interacted with pectin methylesterases and mutations in TMD1 or TMD2 impaired this interaction in planta. The results suggest that the two TMDs regulate the movement function and intracellular transport of 37K. (C) 2012 Elsevier Inc. All rights reserved.

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  • [Plant rhabdoviruses with bipartite genomes].

    Hideki Kondo

    Uirusu   63 ( 2 )   143 - 54   2013年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    Members of the family Rhabdoviridae (order Mononegavirales) have a broad range of hosts, including humans, livestock, fish, plants, and invertebrates. They have a nonsegmented negative-sense RNA as the genome. Orchid fleck virus (OFV) is distributed world-wide on several orchid plants and transmitted by the false spider mite, Brevipalpus californicus. Based on its virions morphology and cytopathic effects in the infected cells, OFV was tentatively placed as unassigned plant rhabdoviruses in the sixth ICTV Report. However, the molecular studies reveled that OFV has a unique two-segmented negative-sense RNA genome that resembles monopartite genomes of plant nucleorhabdoviruses. In this review, we describe the current knowledge on the genome structure and gene expression strategy of OFV, the possible mechanism of nuclear viroplasm formation, and the taxonomical consideration of the virus as well.

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  • The cysteine-rich proteins of beet necrotic yellow vein virus and tobacco rattle virus contribute to efficient suppression of silencing in roots 査読

    Ida Bagus Andika, Hideki Kondo, Masamichi Nishiguchi, Tetsuo Tamada

    JOURNAL OF GENERAL VIROLOGY   93 ( Pt 8 )   1841 - 1850   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Many plant viruses encode proteins that suppress RNA silencing, but little is known about the activity of silencing suppressors in roots. This study examined differences in the silencing suppression activity of different viruses in leaves and roots of Nicotiana benthamiana plants. Infection by tobacco mosaic virus, potato virus Y and cucumber mosaic virus but not potato virus X (PVX) resulted in strong silencing suppression activity of a transgene in both leaves and roots, whereas infection by beet necrotic yellow vein virus (BNYVV) and tobacco rattle virus (TRV) showed transgene silencing suppression in roots but not in leaves. For most viruses tested, viral negative-strand RNA accumulated at a very low level in roots, compared with considerable levels of positive-strand genomic RNA. Co-inoculation of leaves with PVX and either BNYVV or TRV produced an increase in PVX negative-strand RNA and subgenomic RNA (sgRNA) accumulation in roots. The cysteine-rich proteins (CRPs) BNYVV p14 and TRV 16K showed weak silencing suppression activity in leaves. However, when either of these CRPs was expressed from a PVX vector, there was an enhancement of PVX negative-strand RNA and sgRNA accumulation in roots compared with PVX alone. Such enhancement of PVX sgRNAs was also observed by expression of CRPs of other viruses and the well-known suppressors HC-Pro and p19 but not of the potato mop-top virus p8 CRP. These results indicate that BNYVV- and TRV-encoded CRPs suppress RNA silencing more efficiently in roots than in leaves.

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  • A novel quadripartite dsRNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix 査読

    Yu-Hsin Lin, Sotaro Chiba, Akio Tani, Hideki Kondo, Atsuko Sasaki, Satoko Kanematsu, Nobuhiro Suzuki

    VIROLOGY   426 ( 1 )   42 - 50   2012年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Here we report the biological and molecular attributes of a novel dsRNA virus isolated from Rosellinia necatrix, a filamentous phytopathogenic fungus. The virus, termed Rosellinia necatrix quadrivirus 1 (RnQV1), forms rigid spherical particles approximately 45 nm in diameter in infected mycelia. The particles contain 4 dsRNA segments, dsRNA1 to dsRNA4, with a size range of 4.9 to 3.7 kbp, each possessing a single large ORF. A comparison of the virus-infected and -cured isogenic fungal strains suggested that RnQV1 infection has no appreciable phenotypic effects. Phylogenetic analysis using the dsRNA3-encoded RdRp sequence revealed that RnQV1 is more distantly related to quadripartite chrysoviruses than to monopartite totiviruses, and is placed in a distinct group from other mycoviruses. No significant sequence similarities were evident between known proteins and RnQV1 structural proteins shown to be encoded by dsRNA2 or dsRNA4. These suggest that RnQV1 is a novel latent virus, belonging to a new family. (C) 2012 Elsevier Inc. All rights reserved.

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  • The enigmatic genome of Chara australis virus 査読

    Adrian J. Gibbs, Marjo Torronen, Anne M. Mackenzie, Jeffery T. Wood, John S. Armstrong, Hideki Kondo, Tetsuo Tamada, Paul L. Keese

    JOURNAL OF GENERAL VIROLOGY   92 ( Pt 11 )   2679 - 2690   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Most of the genomic sequence of Chara australis virus (CAV), previously called Chara corallina virus, has been determined. It is a ssRNA molecule of 9065 nt with at least four ORFs. At its 5' end is an ORF encoding a protein of 227 kDa, distantly homologous to the multifunctional replicases of benyviruses and rubiviruses. Next is an ORF encoding a protein of 44 kDa, homologous to the helicases of pestiviruses. The third ORF encodes an unmatched protein of 38 kDa that is probably a movement protein. The fourth and 3'-terminal ORF encodes a protein of 17.7 kDa homologous to the coat proteins of tobamoviruses. The short methyltransferase region of the CAV replicase matches only the C-terminal motif of benyvirus methyltransferases. This and other clues indicate that approximately 11 % and 2 % of the 5' and 3' termini of the complete CAV genome, respectively, are missing from the sequence. The aligned amino acid sequences of the CAV proteins and their nearest homologues contain many gaps but relationships inferred from them were little affected by removal of these gaps. Sequence comparisons show that three of the CAV genes may have diverged from the most closely related genes of other viruses 250-450 million years ago, and the sister relationship between the genes of CAV and those of benyviruses and tobamoviruses, mirroring the ancient sister relationship between charophytes (i.e. the algal host of CAV) and embryophytes (i.e. the plant hosts of tobamoviruses and benyviruses), is congruent with this possibility.

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  • Widespread Endogenization of Genome Sequences of Non-Retroviral RNA Viruses into Plant Genomes 査読

    Sotaro Chiba, Hideki Kondo, Akio Tani, Daisuke Saisho, Wataru Sakamoto, Satoko Kanematsu, Nobuhiro Suzuki

    PLOS PATHOGENS   7 ( 7 )   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Non-retroviral RNA virus sequences (NRVSs) have been found in the chromosomes of vertebrates and fungi, but not plants. Here we report similarly endogenized NRVSs derived from plus-, negative-, and double-stranded RNA viruses in plant chromosomes. These sequences were found by searching public genomic sequence databases, and, importantly, most NRVSs were subsequently detected by direct molecular analyses of plant DNAs. The most widespread NRVSs were related to the coat protein (CP) genes of the family Partitiviridae which have bisegmented dsRNA genomes, and included plant-and fungus-infecting members. The CP of a novel fungal virus (Rosellinia necatrix partitivirus 2, RnPV2) had the greatest sequence similarity to Arabidopsis thaliana ILR2, which is thought to regulate the activities of the phytohormone auxin, indole-3-acetic acid (IAA). Furthermore, partitivirus CP-like sequences much more closely related to plant partitiviruses than to RnPV2 were identified in a wide range of plant species. In addition, the nucleocapsid protein genes of cytorhabdoviruses and varicosaviruses were found in species of over 9 plant families, including Brassicaceae and Solanaceae. A replicase-like sequence of a betaflexivirus was identified in the cucumber genome. The pattern of occurrence of NRVSs and the phylogenetic analyses of NRVSs and related viruses indicate that multiple independent integrations into many plant lineages may have occurred. For example, one of the NRVSs was retained in Ar. thaliana but not in Ar. lyrata or other related Camelina species, whereas another NRVS displayed the reverse pattern. Our study has shown that single-and double-stranded RNA viral sequences are widespread in plant genomes, and shows the potential of genome integrated NRVSs to contribute to resolve unclear phylogenetic relationships of plant species.

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  • The Evolutionary History of Beet necrotic yellow vein virus Deduced from Genetic Variation, Geographical Origin and Spread, and the Breaking of Host Resistance 査読

    Soutaro Chiba, Hideki Kondo, Masaki Miyanishi, Ida Bagus Andika, Chenggui Han, Tetsuo Tamada

    MOLECULAR PLANT-MICROBE INTERACTIONS   24 ( 2 )   207 - 218   2011年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYTOPATHOLOGICAL SOC  

    Beet necrotic yellow vein virus (BNYVV) is an economically important pathogen of sugar beet and has been found worldwide, probably as the result of recent worldwide spread. The BNYVV genome consists of four or five RNA components. Here, we report analysis of sequence variation in the RNA3-p25, RNA4-p31, RNA2-CP, and RNA5-p26 genes of 73 worldwide isolates. The RNA3-p25 gene encodes virulence and avirulence factors. These four sets of gene sequences each fell into two to four groups, of which the three groups of p25 formed eight subgroups with different geographical distributions. Each of these subgroup isolates (strains) could have arisen from four original BNYVV population and their mixed infections. The genetic diversity for BNYVV was relatively small. Selection pressure varied greatly depending on the BNYVV gene and geographical location. Isolates of the Italy strain, in which p25 was subject to the strongest positive selection, were able to overcome the Rz1-host resistance gene to differing degrees, whereas other geographically limited strains could not. Resistance-breaking variants were generated by p25 amino acid changes at positions 67 and 68. Our studies suggest that BNYVV originally evolved in East Asia and has recently become a pathogen of cultivated sugar beet followed by the emergence of new resistance-breaking variants.

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  • Identification and characterization of structural proteins of orchid fleck virus 査読

    Hideki Kondo, Takanori Maeda, Tetsuo Tamada

    ARCHIVES OF VIROLOGY   154 ( 1 )   37 - 45   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    Orchid fleck virus (OFV) has a bipartite negative-sense RNA genome with sequence similarities to plant rhabdoviruses. The non-enveloped bullet-shaped particles of OFV are similar to those of the internal ribonucleoprotein (RNP)-M protein structure of rhabdoviruses, but they are about half the size of typical plant rhabdoviruses. Purified preparations contained intact bullet-shaped and filamentous particles. The filamentous particles showed a tightly coiled coil structure or a coiled structure with a helical twist, which resembles the RNP complex of rhabdoviruses. OFV bullet-shaped particles were structurally stable in solutions containing 2% Triton X-100 and 0.8 M NaCl. Western blot analyses revealed that the bullet-shaped particles contained N, P and M proteins, while filamentous particles contained mainly N and P proteins. In addition, a small amount of the L protein was detected in both types of particles. Thus, the structural proteins of OFV have properties similar to those of rhabdoviruses, except that the particles are non-enveloped and are relatively resistant to detergent-treatment under high-salt conditions.

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  • Role of N-terminal His-rich domain of Oscillatoria brevis Bxa1 in both Ag(I)/Cu(I) and Cd(II)/Zn(II) tolerance. 査読

    Nakakihara E, Kondo H, Nakashima S, Ezaki B

    The open microbiology journal   3   15 - 22   2009年

  • The crucial role of mitochondrial regulation in adaptive aluminium resistance in Rhodotorula glutinis 査読

    Akio Tani, Chiemi Inoue, Yoko Tanaka, Yoko Yamamoto, Hideki Kondo, Syuntaro Hiradate, Kazuhide Kimbara, Fusako Kawai

    MICROBIOLOGY-SGM   154 ( Pt 11 )   3437 - 3446   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Rhodotorula glutinis IFO1125 was found to acquire increased aluminium (Al) resistance from 50 mu M to more than 5 mM by repetitive culturing with stepwise increases in Al concentration at pH 4.0. To investigate the mechanism underlying this novel phenomenon, wild-type and Al- resistant cells were compared. Neither cell type accumulated the free form of Al (Al3+) added to the medium. Transmission electron microscopic analyses revealed a greater number of mitochondria in resistant cells. The formation of small mitochondria with simplified cristae structures was observed in the wild-type strain grown in the presence of Al and in resistant cells grown in the absence of Al. Addition of Al to cells resulted in high mitochondrial membrane potential and concomitant generation of reactive oxygen species (ROS). Exposure to Al also resulted in elevated levels of oxidized proteins and oxidized lipids. Addition of the antioxidants a-tocopherol and ascorbic acid alleviated the Al toxicity, suggesting that ROS generation is the main cause of Al toxicity. Differential display analysis indicated upregulation of mitochondrial genes in the resistant cells. Resistant cells were found to have 2.5- to 3-fold more mitochondrial DNA (mtDNA) than the wild-type strain. Analysis of tricarboxylic acid cycle and respiratory-chain enzyme activities in wild-type and resistant cells revealed significantly reduced cytochrome c oxidase activity and resultant high ROS production in the latter cells. Taken together, these data suggest that the adaptive increased resistance to Al stress in resistant cells resulted from an increased number of mitochondria and increased mtDNA content, as a compensatory response to reduced respiratory activity caused by a deficiency in complex IV function.

    DOI: 10.1099/mic.0.2007/016048-0

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  • Mitochondrial alterations related to programmed cell death in tobacco cells under aluminium stress 査読

    Sanjib Kumar Panda, Yoko Yamamoto, Hideki Kondo, Hideaki Matsumoto

    COMPTES RENDUS BIOLOGIES   331 ( 8 )   597 - 610   2008年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER  

    The present investigation was undertaken to verify whether mitochondria play a significant role in aluminium(Al) toxicity, using the mitochondria isolated from tobacco cells (Nicotiana tabacum, non-chlorophyllic cell line SL) under Al stress. An inhibition of respiration was observed in terms of state-III, state-IV, succinate-dependent, alternative oxidase (AOX)-pathway capacity and cytochrome (CYT)-pathway capacity, respectively, in the mitochondria isolated from tobacco cells subjected to Al stress for 18 h. In accordance with the respiratory inhibition, the mitochondrial ATP content showed a significant decrease under Al treatment. An enhancement of reactive oxygen species (ROS) production under state-III respiration was observed in the mitochondria isolated from Al-treated cells, which would create an oxidative stress situation. The opening of mitochondrial permeability transition pore (MPTP) was seen more extensively in mitochondria isolated from Al-treated cells than in those isolated from control cells. This was Ca2+ dependent and well modulated by dithioerythritol (DTE) and Pi, but insensitive to cyclosporine A (CsA). The collapse of inner mitochondrial membrane potential (Delta Psi(m)) was also observed with a release of cytochrome c from mitochondria. A great decrease in the ATP content was also seen under Al stress. Transmission electron microscopy analysis of Al-treated cells also corroborated our biochemical data with distortion in membrane architecture in mitochondria. TUNEL-positive nuclei in Al-treated cells strongly indicated the occurrence of nuclear fragmentation. From the above study, it was concluded that Al toxicity affects severely the mitochondrial respiratory functions and alters the redox status studied in vitro and also the internal structure, which seems to cause finally cell death in tobacco cells.

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  • Identification of amino acids of the beet necrotic yellow vein virus p25 protein required for induction of the resistance response in leaves of Beta vulgaris plants 査読

    Soutaro Chiba, Masaki Miyanishi, Ida Bagus Andika, Hideki Kondo, Tetsuo Tamada

    JOURNAL OF GENERAL VIROLOGY   89 ( Pt 5 )   1314 - 1323   2008年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MICROBIOLOGY SOC  

    The RNA3-encoded p25 protein of beet necrotic yellow vein virus (BNYVV) is responsible for the production of rhizomania symptoms of sugar beet roots (Beta vulgaris subsp. vulgaris). Here, it was found that the presence of the p25 protein is also associated with the resistance response in rub-inoculated leaves of sugar beet and wild beet (Beta vulgaris subsp. maritima) plants. The resistance phenotype displayed a range of symptoms from no visible lesions to necrotic or greyish lesions at the inoculation site, and only very low levels of virus and viral RNA accumulated. The susceptible phenotype showed large, bright yellow lesions and developed high levels of virus accumulation. In roots after Polymyxa betae vector inoculation, however, no drastic differences in virus and viral RNA accumulation levels were found between plants with susceptible and resistant phenotypes, except at an early stage of infection. There was a genotype-specific interaction between BNYVV strains and two selected wild beet lines (MR1 and MR2) and sugar beet cultivars. Sequence analysis of natural BNYVV isolates and site-directed mutagenesis of the p25 protein revealed that 3 aa residues at positions 68, 70 and 179 are important in determining the resistance phenotype, and that host-genotype specificity is controlled by single amino acid changes at position 68. The mechanism of the occurrence of resistance-breaking BNYVV strains is discussed.

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  • RNA4-encoded p31 of beet necrotic yellow vein virus is involved in efficient vector transmission, symptom severity and silencing suppression in roots 査読

    Muhammad Danial Rahim, Ida Bagus Andika, Chenggui Han, Hideki Kondo, Tetsuo Tamada

    JOURNAL OF GENERAL VIROLOGY   88 ( Pt 5 )   1611 - 1619   2007年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    RNA3 and RNA4 of beet necrotic yellow vein virus (BNYVV) are not essential for virus multiplication, but are associated with vector-mediated infection and disease development in sugar beet roots. Here, a unique role for RNA4 in virus transmission, virulence and RNA silencing suppression was demonstrated. Mutagenic analysis revealed that the RNA4-encoded p31 open reading frame (ORF) was involved in efficient vector transmission and slight enhancement of symptom expression in some Beta species. No effects of RNA4 on virus accumulation in infected tissue were observed. Furthermore, the p31 ORF was involved in the induction of severe symptoms by BNYVV in Nicotiana benthamiana plants without affecting viral RNA accumulation. In contrast, RNA3-encoded p25, previously identified as a major contributor to symptom induction in sugar beet, had no such effect on N. benthamiana. In two different silencing suppression assays, neither p31 nor p25 was able to suppress RNA silencing in leaves, but the presence of p31 enhanced a silencing suppressor activity in roots without alteration in viral RNA accumulation. Thus, BNYVV p31 plays a multifunctional role in efficient vector transmission, enhanced symptom expression and root-specific silencing suppression.

    DOI: 10.1099/vir.0.82720-0

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  • Orchid fleck virus is a rhabdovirus with an unusual bipartite genome 査読

    Hideki Kondo, Takanori Maeda, Yukio Shirako, Tetsuo Tamada

    JOURNAL OF GENERAL VIROLOGY   87 ( Pt 8 )   2413 - 2421   2006年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Orchid fleck virus (OFV) has an unusual bipartite negative-sense RNA genome with clear sequence similarities to those of nucleorhabdoviruses. The OFV genome consists of two single-stranded RNA molecules, RNA1 and RNA2 that are 6413 and 6001 nt long, respectively, with open reading frame (ORF) information in the complementary sense. RNA1 encodes 49 (ORF1), 26 (ORF2), 38 (ORF3), 20 (ORF4) and 61 kDa (ORF5) proteins, and RNA2 encodes a single protein of 212 kDa (ORF6). ORF1, ORF5 and ORF6 proteins had significant similarities (21-38 % identity) to the nucleocapsid protein (N), glycoprotein (G) and polymerase (L) gene products, respectively, of other rhabdoviruses, especially nucleorhabdoviruses, whereas ORF2, ORF3 and ORF4 proteins had no significant similarities to other proteins in the international databases. Similarities between OFV and rhabdoviruses were also found in the sequence complementarity at both termini of each RNA segment (the common terminal sequences are 3'-UGUGUC---GACACA-5'), the conserved intergenic sequences and in being negative sense. It was proposed that a new genus Dichorhabdovirus in the family Rhabdoviridae of the order Mononegavirales should be established with OFV as its prototype member and type species.

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  • Lower levels of transgene silencing in roots is associated with reduced DNA methylation levels at non-symmetrical sites but not at symmetrical sites 査読

    IB Andika, H Kondo, MD Rahim, T Tamada

    PLANT MOLECULAR BIOLOGY   60 ( 3 )   423 - 435   2006年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Transgene transcripts were recently shown to accumulate at higher levels in roots, relative to leaves, of silenced-transgenic Nicotiana benthamiana plants and to be inversely related with the accumulation of small interfering RNAs (siRNAs), suggesting that RNA silencing is less active in roots than in leaves (Andika et al., 2005. Mol. Plant-Microbe Interact. 18: 194). Here we show that the lower transgene RNA silencing activity in roots was associated with lower transgene methylation levels at non-symmetrical CpNpN context but not at symmetrical CpG or CpNpG context in three sets of transformant plants with different exogenous genes. In contrast, such a difference between roots and leaves was not observed for the Tnt1 retrotransposon: no Tnt1 transcript was detected in roots or in leaves of N. benthamiana, while equal levels of Tnt1-derived siRNA accumulation and Tnt1 methylation were found. From our data and previously reported information, we suggest that roots have less of an activity that acts at the step of generation of siRNAs.

    DOI: 10.1007/s11103-005-4429-7

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  • Evidence that RNA silencing-mediated resistance to Beet necrotic yellow vein virus is less effective in roots than in leaves 査読

    IB Andika, H Kondo, T Tamada

    MOLECULAR PLANT-MICROBE INTERACTIONS   18 ( 3 )   194 - 204   2005年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYTOPATHOLOGICAL SOC  

    In plants, RNA silencing is part of a defense mechanism against virus infection but there is little information as to whether RNA silencing-mediated resistance functions similarly in roots and leaves. We have obtained transgenic Nicotiana benthamiana plants encoding the coat protein readthrough domain open reading frame (54 kDa) of Beet necrotic yellow vein virus (BNYVV), which either showed a highly resistant or a recovery phenotype following foliar rub-inoculation with BNYVV. These phenotypes were associated with an RNA silencing mechanism. Roots of the resistant plants that were immune to foliar rub-inoculation with BNYVV could be infected by viruliferous zoo-spores of the vector fungus Polymyxa betae, although virus multiplication was greatly limited. In addition, virus titer was reduced in symptomless leaves of the plants showing the recovery phenotype, but it was high in roots of the same plants. Compared with leaves of silenced plants, higher levels of transgene mRNAs and lower levels of transgene-derived small interfering RNAs (siRNAs) accumulated in roots. Similarly, in nontransgenic plants inoculated with BNYVV, accumulation level of viral RNA-derived siRNAs in roots was lower than in leaves. These results indicate that the RNA silencing-mediated resistance to BNYVV is less effective in roots than in leaves.

    DOI: 10.1094/MPMI-18-0194

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  • A reovirus of the fungus Cryphonectria parasitica that is infectious as particles and related to the Coltivirus genus of animal pathogens 査読

    BI Hillman, S Supyani, H Kondo, N Suzuki

    JOURNAL OF VIROLOGY   78 ( 2 )   892 - 898   2004年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    RNA viruses of filamentous fungi fall into two broad categories, those that contain double-stranded RNA (dsRNA) genomes in rigid particles and those that are more closely related to positive-sense, single-stranded RNA viruses with dsRNA replicative intermediates found within lipid vesicles. Effective infectivity systems have been described for the latter, using RNA transcripts, but not for the former. We report the characterization of a reovirus from Cryphonectria parasitica, the filamentous fungus that causes chestnut blight disease. The virus substantially reduces the virulence of the fungus and results in dramatically altered colony morphology, as well as changes in other associated fungal traits, relative to the virus-free isogenic strain. Virus particles from infected mycelium contained 11 segments of dsRNA and showed characteristics typical of the family Reoviridae. Sequences of the largest three segments revealed that the virus is closely related to the Coltivirus genus of animal pathogens, which includes the human pathogen Colorado tick fever virus. The introduction of purified virus particles into protoplasts from virus-free isolates of the fungus resulted in a newly infected mycelium with the same morphology and virus composition as the original virus-infected isolate. This represents the completion of Koch's postulates for a true dsRNA virus from a filamentous fungus and the description of a definitive fungal member of the family Reoviridae.

    DOI: 10.1128/JVI.78.2.892-898.2004

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  • Orchid fleck virus: Brevipalpus californicus mite transmission, biological properties and genome structure 査読 国際誌

    H Kondo, T Maeda, T Tamada

    EXPERIMENTAL AND APPLIED ACAROLOGY   30 ( 1-3 )   215 - 223   2003年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KLUWER ACADEMIC PUBL  

    Orchid fleck virus (OFV) causes necrotic or chlorotic ring spots and fleck symptoms in many orchid species world-wide. The virus has non-enveloped, bacilliform particles of about 40 nm x 100-150 nm and is sap-transmissible to several plant species. OFV is transmitted by the mite Brevipalpus californicus (Banks) in a persistent manner and efficiently transmitted by both adults and nymphs, but not by larvae. Viruliferous mites retain their infectivity for 3 weeks on a virus-immune host. The genome of OFV consists of two molecules of 6431 (RNA1) and 6001 nucleotides (RNA2). The RNAs have conserved and complementary terminal sequences. RNA1 contains five open reading frames (ORF), and RNA2 encodes a single ORF. Although some of the encoded proteins of OFV have sequences similar to those of proteins of plant rhabdoviruses, OFV differs from viruses in the family Rhabdoviridae in having a bipartite genome.

    DOI: 10.1023/B:APPA.0000006550.88615.10

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  • Identification of Orchid fleck virus by reverse transcriptase-polymerase chain reaction and analysis of isolate relationships

    AL Blanchfield, AM Mackenzie, A Gibbs, H Kondo, T Tamada, CR Wilson

    JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT   149 ( 11-12 )   713 - 718   2001年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL VERLAG GMBH  

    Using reverse transcription-polymerase chain reaction (RT-PCR) tests with a primer complementary to a region of its nucleoprotein gene together with a polydT/ SP6 primer, Orchid fleck virus (OFV) was detected in 34 samples of infected orchids of several different genera from world-wide sources. The resulting DNA fragments were approximately 800 bp in length and their sequences were determined directly. Analysis of sequences of the major open reading frame (ORF) within the DNA fragments obtained showed the presence of two virus strains. The first group was of two isolates, the original Japanese isolate from which the primers were derived and one from Germany, and the second group contained 33 isolates from four continents. The sequences of different groups differed from one another by at least 15.6% (nucleotide) and 1.8% (amino-acid), but the within-group differences were much less (< 1.7% difference). A search of the international nucleotide database with the OFV sequences showed them to be related, but distantly, to nucleoprotein regions of the genomes of four plant-infecting rhabdoviruses.

    DOI: 10.1046/j.1439-0434.2001.00702.x

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    J-GLOBAL

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  • Comparative cytopathology and immunocytochemistry of Japan, Australian and Brazilian isolates of Orchid fleck virus.(共著)

    Kitajima et al.

    Journal of General Plant Pathology   67   231 - 237   2001年

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  • Calanthe mild mosaic virus, a new potyvirus causing a mild mosaic disease of Clanth orchid in Japan(共著)

    Garae t al

    Journal of Phytopathology   146 ( 7 )   357 - 363   1998年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Protective roles of two aluminum (Al)-induced genes, HSP150 and SED1 of Saccharomyces cerevisiae, in Al and oxidative stresses

    B Ezaki, RC Gardner, Y Ezaki, H Kondo, H Matsumoto

    FEMS MICROBIOLOGY LETTERS   159 ( 1 )   99 - 105   1998年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We isolated two yeast cDNA clones whose transcripts are induced by aluminum (Al) metal stress. Partial nucleotide sequencing showed that one is the HSP150 gene encoding a secreted heat shock protein, and the other corresponds to the SED1 gene encoding a putative membrane protein. To clarify the biological functions of these genes, we analyzed the sensitivity of gene-disrupted mutants to Al stress and to oxidative stresses. The Al tests indicated that the HSP150 protein served a basal protective role in Al stress, but SED1 did nor, both of the genes had protective roles for oxidative stresses. The results for the HSP150 gene suggest that there is an overlap between Al ion stress, oxidative stress and heat shock stress in yeast. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0378-1097(97)00554-5

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  • Stunt disease of Habenaria radiata caused by a strain of watermelon mosaic virus 2(共著)

    Gara et al.

    Annals of the phytopathological society of Japan   63 ( 2 )   113 - 117   1997年

  • Altered mitochondrial gene expression in a maternal distorted leaf mutant of Arabidopsis induced by chloroplast mutator

    W Sakamoto, H Kondo, M Murata, F Motoyoshi

    PLANT CELL   8 ( 8 )   1377 - 1390   1996年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT PHYSIOLOGISTS  

    chloroplast mutator (chm) of Arabidopsis is a recessive nuclear mutation that causes green and white variegation in leaves and is inherited in a non-Mendelian fashion. In this study, we have identified and characterized a mutant observed in F-1 and backcrossed BC1 populations from a cross between chm1-3 and ecotype Columbia. This mutant, maternal distorted leaf (MDL), grows very poorly and is distinguished by distorted rough leaves and aborted flowering organs. Electron microscopic observation showed that in MDL plants, a significant portion of mitochondria are abnormal and appear to be nonfunctional. DNA gel blot end sequence analysis of the MDL mitochondrial DNA (mtDNA) revealed rearrangements in two mtDNA fragments associated with rps3-rpl16 genes (encoding ribosomal proteins S3 and L16, respectively). One rearrangement resulted in the insertion of the rps3-rpl16 operon downstream of atp9. An independent deletion in this region had eliminated the majority of rps3. In contrast, another rearrangement deleted part of rpl16, whereas rps3 remained intact. RNA gel blot analysis indicated that expression of these genes is also altered as a consequence of the mtDNA rearrangements. Thus, a mutation at the CHM locus affects mitochondrial gene expression, and impaired mitochondrial function may result in the distorted phenotype.

    DOI: 10.1105/tpc.8.8.1377

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  • Ixiaから分離されたbean yellow mosaic virus

    辻 俊也, 前田 孚憲, 近藤 秀樹

    岡山大学資源生物科学研究所報告   4 ( 2 )   201 - 213   1996年3月

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    記述言語:日本語   出版者・発行元:岡山大学資源生物科学研究所  

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書籍等出版物

  • The Border Between Kitavirids and Nege-Like Viruses: Tracking the Evolutionary Pace of Plant- and Arthropod-Infecting Viruses (Frontiers in Plant Science)

    Pedro Luis Ramos-González, Hideki Kondo, Sergey Morozov, Nikolaos Vasilakis, Arvind Varsani, Mengji Cao, Juliana Freitas-Astúa( 担当: 共著 ,  範囲: Editorial:)

    Frontiers Media SA  2022年 

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  • Quadriviruses (Quadriviridae) /Encyclopedia of Virology 4th edition

    Kondo, K, Castón, JR, Suzuki, N( 担当: 分担執筆)

    Elsevier  2021年 

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  • Chapter 5: Dichorhaviruses in their Host Plants and Mite Vectors (Advances in Virus Research 102, 119-148), Edited by P. Palukaitis, M.J. Roossinck

    Dietzgen, R., Freitas-Astúa, J., Chabi-Jesus, C., Ramos-Gonzalez, P.L., Goodin, M., Kondo, H., Tassi, A.D., Kitajima, E.W.

    Academic Press  2018年 

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    総ページ数:v.   記述言語:英語

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  • Plant Virology Protocols: Detection and analysis for non-retroviral RNA virus-like elements in plant, fungal and insect genomes (Methods in Molecular Biology 1236, 73-88), eds, Uyeda, I and Masuta C.

    Kondo, H, Chiba, S, Suzuki, N

    Springer Protocol, Humana Press  2015年  ( ISBN:9781493917426

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    総ページ数:xi, 292 p.   記述言語:英語

    CiNii Books

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  • Mycoviruses. Chapter 7: Viruses of the white root rot fungus, Rosellinia necatrix (Advances in Virus Research 86: 177-214), Editor: S Ghabrial.

    Kondo, H., Kanematsu, S. and Suzuki, N

    Academic Press  2013年  ( ISBN:9780123943156

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    総ページ数:xiv, 362 p., [24] p. of plates.   記述言語:英語

    CiNii Books

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  • Cross-Kingdom Virus Infection / Encyclopedia of Virology 4th edition

    Sun, L, Kondo, H, Andika, IB( 担当: 分担執筆)

    Elsevier  2021年 

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    著書種別:事典・辞書

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  • ランのウイルス病 -最新農業技術 花卉- vol.12

    近藤秀樹 (改訂)

    農山漁村文化協会  2020年  ( ISBN:9784540190599

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    総ページ数:4, 228p   記述言語:日本語

    CiNii Books

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  • Rhizomania. Chapter 5:Genetic diversity of beet necrotic yellow vein virus

    Tamada, T, Kondo, K, Chiba, S

    Springer, Heidel-berg.  2016年 

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  • 植物ウイルス大事典. デンドロビウムモザイクウイルス(分担).

    近藤秀樹

    朝倉書店. 編集 日比, 忠明;大木, 理  2015年11月  ( ISBN:9784254420401

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    総ページ数:xxxiv, 904p   記述言語:日本語

    CiNii Books

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  • サギソウ萎縮病(分担)/インターネット版日本植物病害大事典 病害新情報

    近藤 秀樹

    全農教  2013年 

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  • シュンラン退緑斑病(分担)/インターネット版日本植物病害大事典 病害新情報

    近藤 秀樹

    全農教  2013年 

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MISC

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講演・口頭発表等

▼全件表示

Works(作品等)

  • 「本邦の稀少ラン科植物に発生するウイルスの性状解明と遺伝子診断技術の開発」八雲環境科学振興財団 環境研究助成

    近藤 秀樹

    2015年

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  • 「ゲノム上に存在する非レトロウイルス様配列NRVSの特徴付けと存在意義に関する研究」山陽放送学術文化財団研究助成

    近藤 秀樹

    2013年

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  • 「マイナス鎖RNAウイルス研究の新展開-ウイルス化石より見出された菌類ウイルスの実態を解き明かす-」両備檉園記念財団研究助成

    近藤 秀樹

    2013年

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  • 土壌菌により媒介される植物病原性ウイルスの新規防除戦略の構築:「RNAサイレンシングによるウイルスの制御をめざして」ウエスコ学術振興財団研究助成

    近藤 秀樹

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受賞

  • 名古屋国際蘭会議2010 NIOC賞

    2010年  

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    受賞国:日本国

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  • 名古屋国際蘭会議2009 NIOC奨励賞

    2009年  

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    受賞国:日本国

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共同研究・競争的資金等の研究

  • マイコイミュニティ研究の最前線とその植物病理学への新展開

    研究課題/領域番号:21H05035  2021年07月 - 2026年03月

    日本学術振興会  科学研究費助成事業 基盤研究(S)  基盤研究(S)

    鈴木 信弘, 近藤 秀樹, 河野 洋治

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    配分額:188240000円 ( 直接経費:144800000円 、 間接経費:43440000円 )

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  • 植物・菌・動物の生物界を跨いだウイルス感染:宿主との攻防と適応戦略

    研究課題/領域番号:21K18222  2021年07月 - 2025年03月

    日本学術振興会  科学研究費助成事業 挑戦的研究(開拓)  挑戦的研究(開拓)

    鈴木 信弘, 近藤 秀樹

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    配分額:25870000円 ( 直接経費:19900000円 、 間接経費:5970000円 )

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  • モデル糸状菌アカパンカビを用いたウイルス研究フロンティア

    研究課題/領域番号:21K19086  2021年07月 - 2023年03月

    日本学術振興会  科学研究費助成事業 挑戦的研究(萌芽)  挑戦的研究(萌芽)

    近藤 秀樹

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    配分額:6500000円 ( 直接経費:5000000円 、 間接経費:1500000円 )

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  • 菌類ウイルスによる焼酎生産菌の一次・二次代謝産物生産機構に関する研究

    研究課題/領域番号:20K05791  2020年04月 - 2023年03月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    藤森 文啓, 近藤 秀樹, 千葉 壮太郎

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    配分額:4290000円 ( 直接経費:3300000円 、 間接経費:990000円 )

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  • 作物根圏におけるウイルス叢の多様性とその感染動態から紐解く生態的意義

    研究課題/領域番号:20H02987  2020年04月 - 2023年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    近藤 秀樹, 久野 裕, 兵頭 究, 鈴木 信弘

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    配分額:17680000円 ( 直接経費:13600000円 、 間接経費:4080000円 )

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  • 植物糸状菌の抗ウイルス免疫機構のフロンティア

    研究課題/領域番号:17H01463  2017年04月 - 2021年03月

    日本学術振興会  科研費・基盤研究A  基盤研究(A)

    鈴木信弘, 近藤秀樹

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    資金種別:競争的資金

    「RNA干渉機構(RNAi)」による菌類ウイルスに対する自然免疫機構(複製阻害)を植物病原糸状菌で解析し、ウイルス防御機構の研究における植物・動物界に次ぐ第三極の形成を目指した。複数の予想もし得なかった結果を得た。すなわち、ウイルス特異的なDicer依存AGO不要の抗ウイルスRNAiの発見、宿主特異的ウイルス干渉機構の発見、新規ウイルス防御機構としての病徴発現軽減機構の発見、病徴発現軽減に関わる宿主遺伝子のDicerおよびSAGA複合体(ユニバーサル転写コアクティベーター)の同定、に至った。これらはパラダイムシフトをもたらす成果であり、一流専門誌あるいは一般誌に公表に至った。

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  • 糸状菌ウイルスのネオ・ライフスタイル

    研究課題/領域番号:16H06436  2016年06月 - 2021年03月

    日本学術振興会  科研費・新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    鈴木信弘, 近藤秀樹

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    資金種別:競争的資金

    糸状菌ウイルスの2つのネオライフスタイルを確立し、それらの普遍性そして生態学的意義を検討した。まず、宿借・宿主性(ウイルス間の全く新しい相利共生関係)の証明とその分子基盤を紐解いた。ヤドカリウイルスはヤドヌシウイルスのキャプシドをハイジャックし、自身のRNA合成酵素を使って複製する、一方ヤドヌシウイルスの複製を促進することを証明した。さらに、裸性(粒子は作らず、裸のRNAで存在)を示すハダカウイルスはキャプシドレスで感染菌糸破砕液中ではRNase感受性、超遠心で沈降不可の状態で存在することを明らかにした。宿借・宿主性を示す植物ウイルス(他グループによる)も見つかり、その普遍性が示された。

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  • マイコイミュニティ研究の最前線とその植物病理学への新展開

    研究課題/領域番号:21H04727  2021年04月 - 2025年03月

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    鈴木 信弘, 近藤 秀樹, 兵頭 究

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    配分額:41600000円 ( 直接経費:32000000円 、 間接経費:9600000円 )

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  • メタゲノム解析に基づく菌類ウイルス叢の解明と有効利用に関する研究

    研究課題/領域番号:15K07312  2015年04月 - 2018年03月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    近藤 秀樹, 鈴木 信弘

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    配分額:5070000円 ( 直接経費:3900000円 、 間接経費:1170000円 )

    菌類ウイルスの同定はウイルスの多様性や進化の理解に大きく寄与するが,うどんこ病菌(子嚢菌類)やさび病菌(担子菌類)のような活物寄生菌ではウイルスの存在は長らく不明であった。 本課題では、赤クローバーうどんこ病菌から二本鎖RNAを取得し,次世代シークエンサーによりウイルス叢解析を行った。その結果,赤クローバーうどんこ病菌に9種以上の新規トティウイルスの存在が明らかになった。さらに,類似するトティウイルス様配列をマメ科のさび病菌の転写物ライブラリーに見いだした. これらの成果は,トティウイルスがうどんこ病菌とさび病菌の間を嘗て水平伝搬し,それぞれの宿主と共進化したとことを示唆している.

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  • 植物病原糸状菌の抗ウイルス自然免疫機構

    研究課題/領域番号:25252011  2013年04月 - 2017年03月

    日本学術振興会  科研費・基盤研究A  基盤研究(A)

    鈴木信弘

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    資金種別:競争的資金

    本研究では、「RNA干渉機構(RNAi)」による菌類ウイルスに対する自然免疫機構(複製阻害)を植物病原糸状菌で解析し、ウイルス防御機構の研究における植物・動物界に次ぐ第三極の形成を目指した。その結果、予想しなかった大きな成果が得られた。AGOを必要としないウイルス防御、AGOは本来細胞レベルウイルス防御に関わるRNAiの鍵因子であるが、RNAiに対して抑制的に働くAGOの発見、ウイルスのパターン認識からRNAi誘導に関与する宿主因子探索のスクリーニング系の構築等、の成果は抗ウイルスRNAiのパラダイムシフトをもたらす可能性が非常に高い。上記の成果は、一流専門誌あるいは一般誌に公表した。

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  • 植物ゲノムに存在する非レトロウイルス様配列の挿入メカニズムと病理学的意義

    2012年 - 2014年

    科学研究費補助金・基盤研究C 

    近藤秀樹

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    担当区分:研究代表者  資金種別:競争的資金

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  • 植物マイナス鎖RNAウイルスが誘導するバイロプラズムの形成機構と感染戦略上の役割

    2009年 - 2011年

    科学研究費補助金・基盤研究C 

    近藤秀樹

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    担当区分:研究代表者  資金種別:競争的資金

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  • 植物マイナス鎖RNAウイルスのゲノム輸送機構に関する研究

    2007年 - 2008年

    科研費・基盤研究C 

    近藤 秀樹

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    担当区分:研究代表者  資金種別:競争的資金

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  • Benyvirus属ウイルスの菌伝搬機構の解析

    科研費・基盤研究C (分担) 

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    資金種別:競争的資金

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  • 担子菌ウイルスの宿主に及ぼすシグナル経路の解明と子実体形成への機能解析研究

    科研費・基盤研究C (分担) 

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    資金種別:競争的資金

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  • 節足動物により媒介される植物ウイルスの伝搬機構の解析

    科研費・若手B 

    近藤 秀樹

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    担当区分:研究代表者  資金種別:競争的資金

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  • マイナス鎖RNAウイルス遺伝子操作系の確立に関する基礎的研究. 文部省科研費

    科研費・奨励研究A 

    近藤 秀樹

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    担当区分:研究代表者  資金種別:競争的資金

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  • マイコウイルスの分子生物学

    生研センター・イノベーション創出基礎的研究推進事業 

    近藤 秀樹

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    担当区分:研究代表者  資金種別:競争的資金

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  • 病原性・非病原性遺伝子として機能するBNYVV P25タンパク質の解析

    科研費・特定領域研究A公募 (分担) 

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    資金種別:競争的資金

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  • Polymyxa菌によって媒介される植物ウイルスの伝搬機構

    科研費・基盤研究B (分担) 

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    資金種別:競争的資金

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  • 野生植物のウイルス抵抗性遺伝子の検索・評価と抵抗性機構の解明

    科研費・基盤研究C (分担) 

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    資金種別:競争的資金

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▼全件表示

 

担当授業科目

  • ウイルス分子生物学演習 (2021年度) 前期  - その他

  • ウイルス分子生物学演習 (2021年度) 後期  - その他

  • ウイルス分子生物学演習 (2021年度) 後期  - その他

  • ウイルス分子生物学演習 (2021年度) 前期  - その他

  • 応用植物ウイルス学 (2021年度) 後期  - その他

  • 植物ウイルス学 (2021年度) 第1学期  - 木3,木4

  • 植物-ウイルス/細菌相互作用学 (2021年度) 前期  - 木9~12

  • 生物資源科学特別研究 (2021年度) 通年  - その他

  • ウイルス分子生物学演習 (2020年度) 前期  - その他

  • ウイルス分子生物学演習 (2020年度) 後期  - その他

  • ウイルス分子生物学演習 (2020年度) 後期  - その他

  • ウイルス分子生物学演習 (2020年度) 前期  - その他

  • 応用植物ウイルス学 (2020年度) 後期  - その他

  • 植物ウイルス学 (2020年度) 第1学期  - 木3,木4

  • 植物-ウイルス/細菌相互作用 (2020年度) 前期  - その他

  • 生物資源科学特別研究 (2020年度) 通年  - その他

▼全件表示