Updated on 2025/04/24

写真a

 
KONDO Hideki
 
Organization
Scheduled update Associate Professor
Position
Associate Professor
External link

Degree

  • M. Agriculture ( Okayama University )

Research Interests

  • Plant

  • horizontal transfer

  • virus

  • Vector

  • Insect virus

  • plant virus

  • mycovirus

  • phytopathology

  • evolution

  • RNA silencing

  • molecular evolution

  • Rhabdovirus

  • negative-sense RNA virus

  • endogenous virus-like element

Research Areas

  • Environmental Science/Agriculture Science / Plant protection science

  • Life Science / Applied molecular and cellular biology

Education

Research History

  • -

    2014

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  • Assistant Professor, Research Institute for Bioresources, Okayama University   Institute of Plant Science and Resources

    2007 - 2014

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  • 岡山大学資源生物科学研究所 助手

    1993 - 2006

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Professional Memberships

  • THE PHYTOPATHOLOGICAL SOCIETY OF JAPAN

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  • The Japanese Society for Virology

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Committee Memberships

  • 日本植物病理学会関西部会   事務幹事  

    2023.4 - 2024.3   

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  •   ICTV Totiviridae Study Group  

    2021   

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  •   ICTV Mymonaviridae Study Group  

    2021   

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  • J Gen Plant Pathol   Editor  

    2020.1 - 2021.12   

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  • 日本植物病理学会   植物ウイルス分類委員会委員  

    2018   

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    Committee type:Academic society

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Papers

  • Evidence for the replication of a plant rhabdovirus in its arthropod mite vector. International journal

    Hideki Kondo, Miki Fujita, Paul Telengech, Kazuyuki Maruyam, Kiwamu Hyodo, Aline Daniele Tassi, Ronald Ochoa, Ida Bagus Andika, Nobuhiro Suzuki

    Virus research   351   199522 - 199522   2025.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    Transmission of plant viruses that replicate in the insect vector is known as persistent-propagative manner. However, it remains unclear whether such virus-vector relationships also occur between plant viruses and other biological vectors such as arthropod mites. In this study, we investigated the possible replication of orchid fleck virus (OFV), a segmented plant rhabdovirus, within its mite vector (Brevipalpus californicus s.l.) using quantitative RT-qPCR, western blotting and next-generation sequencing. Time-course RT-qPCR and western blot analyses showed an increasing OFV accumulation pattern in mites after virus acquisition. Since OFV genome expression requires the transcription of polyadenylated mRNAs, polyadenylated RNA fractions extracted from the viruliferous mite samples and OFV-infected plant leaves were used for RNA-seq analysis. In the mite and plant datasets, a large number of sequence reads were aligned to genomic regions of OFV RNA1 and RNA2 corresponding to transcribed viral gene mRNAs. This includes the short polyadenylated transcripts originating from the leader and trailer regions at the ends of the viral genome, which are believed to play a crucial role in viral transcription/replication. In contrast, a low number of reads were mapped to the non-transcribed regions (gene junctions). These results strongly suggested that OFV gene expression occurs both in mites and plants. Additionally, deep sequencing revealed the accumulation of OFV-derived small RNAs in mites, although their size profiles differ from those found in plants. Taken together, our results indicated that OFV replicates within a mite vector and is targeted by the RNA-silencing mechanism.

    DOI: 10.1016/j.virusres.2024.199522

    PubMed

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  • Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses. Reviewed International journal

    Michael Louie R Urzo, Timothy D Guinto, Ana Eusebio-Cope, Bernard O Budot, Mary Jeanie T Yanoria, Gilda B Jonson, Masao Arakawa, Hideki Kondo, Nobuhiro Suzuki

    Viruses   16 ( 7 )   2024.7

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Rice sheath blight, caused by the soil-borne fungus Rhizoctonia solani (teleomorph: Thanatephorus cucumeris, Basidiomycota), is one of the most devastating phytopathogenic fungal diseases and causes yield loss. Here, we report on a very high prevalence (100%) of potential virus-associated double-stranded RNA (dsRNA) elements for a collection of 39 fungal strains of R. solani from the rice sheath blight samples from at least four major rice-growing areas in the Philippines and a reference isolate from the International Rice Research Institute, showing different colony phenotypes. Their dsRNA profiles suggested the presence of multiple viral infections among these Philippine R. solani populations. Using next-generation sequencing, the viral sequences of the three representative R. solani strains (Ilo-Rs-6, Tar-Rs-3, and Tar-Rs-5) from different rice-growing areas revealed the presence of at least 36 viruses or virus-like agents, with the Tar-Rs-3 strain harboring the largest number of viruses (at least 20 in total). These mycoviruses or their candidates are believed to have single-stranded RNA or dsRNA genomes and they belong to or are associated with the orders Martellivirales, Hepelivirales, Durnavirales, Cryppavirales, Ourlivirales, and Ghabrivirales based on their coding-complete RNA-dependent RNA polymerase sequences. The complete genome sequences of two novel RNA viruses belonging to the proposed family Phlegiviridae and family Mitoviridae were determined.

    DOI: 10.3390/v16071152

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  • The intriguing phenomenon of cross-kingdom infections of plant and insect viruses to fungi: Can other animal viruses also cross-infect fungi? Reviewed International journal

    Ida Bagus Andika, Xinran Cao, Hideki Kondo, Liying Sun

    PLoS pathogens   19 ( 10 )   e1011726   2023.10

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Fungi are highly widespread and commonly colonize multicellular organisms that live in natural environments. Notably, studies on viruses infecting plant-associated fungi have revealed the interesting phenomenon of the cross-kingdom transmission of viruses and viroids from plants to fungi. This implies that fungi, in addition to absorbing water, nutrients, and other molecules from the host, can acquire intracellular parasites that reside in the host. These findings further suggest that fungi can serve as suitable alternative hosts for certain plant viruses and viroids. Given the frequent coinfection of fungi and viruses in humans/animals, the question of whether fungi can also acquire animal viruses and serve as their hosts is very intriguing. In fact, the transmission of viruses from insects to fungi has been observed. Furthermore, the common release of animal viruses into the extracellular space (viral shedding) could potentially facilitate their acquisition by fungi. Investigations of the cross-infection of animal viruses in fungi may provide new insights into the epidemiology of viral diseases in humans and animals.

    DOI: 10.1371/journal.ppat.1011726

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  • Cross-Kingdom Interactions Between Plant and Fungal Viruses. Reviewed International journal

    Ida Bagus Andika, Mengyuan Tian, Ruiling Bian, Xinran Cao, Ming Luo, Hideki Kondo, Liying Sun

    Annual review of virology   2023.7

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    The large genetic and structural divergences between plants and fungi may hinder the transmission of viruses between these two kingdoms to some extent. However, recent accumulating evidence from virus phylogenetic analyses and the discovery of naturally occurring virus cross-infection suggest the occurrence of past and current transmissions of viruses between plants and plant-associated fungi. Moreover, artificial virus inoculation experiments showed that diverse plant viruses can multiply in fungi and vice versa. Thus, virus cross-infection between plants and fungi may play an important role in the spread, emergence, and evolution of both plant and fungal viruses and facilitate the interaction between them. In this review, we summarize current knowledge related to cross-kingdom virus infection in plants and fungi and further discuss the relevance of this new virological topic in the context of understanding virus spread and transmission in nature as well as developing control strategies for crop plant diseases. Expected final online publication date for the Annual Review of Virology, Volume 10 is September 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

    DOI: 10.1146/annurev-virology-111821-122539

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  • Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan Reviewed

    Hideki Kondo, Hitomi Sugahara, Miki Fujita, Kiwamu Hyodo, Ida Bagus Andika, Hiroshi Hisano, Nobuhiro Suzuki

    Pathogens   12 ( 3 )   358 - 358   2023.2

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Many aphid-borne viruses are important pathogens that affect wheat crops worldwide. An aphid-transmitted closterovirus named wheat yellow leaf virus (WYLV) was found to have infected wheat plants in Japan in the 1970s; however, since then, its viral genome sequence and occurrence in the field have not been investigated. We observed yellowing leaves in the 2018/2019 winter wheat-growing season in an experimental field in Japan where WYLV was detected five decades ago. A virome analysis of those yellow leaf samples lead to the discovery of a closterovirus together with a luteovirus (barley yellow dwarf virus PAV variant IIIa). The complete genomic sequence of this closterovirus, named wheat closterovirus 1 isolate WL19a (WhCV1-WL19a), consisted of 15,452 nucleotides harboring nine open reading frames. Additionally, we identified another WhCV1 isolate, WL20, in a wheat sample from the winter wheat-growing season of 2019/2020. A transmission test indicated that WhCV1-WL20 was able to form typical filamentous particles and transmissible by oat bird-cherry aphid (Rhopalosiphum pad). Sequence and phylogenetic analyses showed that WhCV1 was distantly related to members of the genus Closterovirus (family Closteroviridae), suggesting that the virus represents a novel species in the genus. Furthermore, the characterization of WhCV1-WL19a-derived small RNAs using high-throughput sequencing revealed highly abundant 22-nt-class small RNAs potentially derived from the 3′-terminal end of the WhCV1 negative-strand genomic RNA, indicating that this terminal end of the WhCV1 genome is likely particularly targeted for the synthesis of viral small RNAs in wheat plants. Our results provide further knowledge on closterovirus diversity and pathogenicity and suggest that the impact of WhCV1 on wheat production warrants further investigations.

    DOI: 10.3390/pathogens12030358

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Books

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MISC

  • ランのウイルス病 -最新農業技術 花卉- vol.12

    近藤秀樹 (改訂)

    2020

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    Language:Japanese   Publisher:農山漁村文化協会  

    CiNii Books

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  • 植物ウイルス大事典. デンドロビウムモザイクウイルス(分担).

    近藤秀樹

    2015.11

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    Language:Japanese   Publisher:朝倉書店. 編集 日比, 忠明;大木, 理  

    CiNii Books

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  • 宿主ゲノム上に存在するRNAウイルス感染記録を紐解く

    近藤秀樹, 千葉壮太郎, 千葉壮太郎, 鈴木信弘

    日本植物病理学会植物感染生理談話会論文集   ( 50 )   133‐142   2015.8

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    Language:Japanese  

    J-GLOBAL

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  • テンサイそう根病の病原ウイルス(BNYVV)の進化と品種抵抗性

    玉田哲男, 近藤秀樹

    植物防疫   68 ( 4 )   168 - 179   2014.4

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  • 分節型ゲノムを持つラブドウイルス

    近藤秀樹

    ウイルス   63 ( 2 )   143 - 154   2013.12

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Presentations

  • Characterization of a closterovirus from wheat plants with yellowing leaf symptoms in Japan

    近藤秀樹, 兵頭究, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集  2024 

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    Event date: 2024

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  • Virome analysis of aphid populations that infest the barley field

    近藤秀樹, 藤田美貴, 久野裕, 兵頭究, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集  2021 

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    Event date: 2021

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  • Two novel negative-strand RNA mycoviruses related to mymonaviruses and phenuiviruses in the Shiitake mushroom

    Kondo, H, Lin, Y-H, Fujita, M, Hyodo, K, Andika, I.B, Suzuki, N

    Asian Mycology 2019 in Mie, Satellite Meeting: Neo-mycovirology.  2019 

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    Event date: 2019.10.4

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  • 分節型植物ラブドウイルスは媒介者である節足動物のダニ体内で増殖する

    近藤秀樹, 兵頭 究, 鈴木信弘

    令和6年度日本植物病理学会大会  2025.3.26 

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  • Evidence for the replication of a bipartite plant rhabdovirus in its arthropod mite vector.

    Kondo, H., Hyodo, K., Suzuki, N.

    The 5th Korea-Japan Joint Symposium on Plant Pathology  2025.3.25 

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Works

  • 「本邦の稀少ラン科植物に発生するウイルスの性状解明と遺伝子診断技術の開発」八雲環境科学振興財団 環境研究助成

    近藤 秀樹

    2015

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  • 「ゲノム上に存在する非レトロウイルス様配列NRVSの特徴付けと存在意義に関する研究」山陽放送学術文化財団研究助成

    近藤 秀樹

    2013

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  • 「マイナス鎖RNAウイルス研究の新展開-ウイルス化石より見出された菌類ウイルスの実態を解き明かす-」両備檉園記念財団研究助成

    近藤 秀樹

    2013

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  • 土壌菌により媒介される植物病原性ウイルスの新規防除戦略の構築:「RNAサイレンシングによるウイルスの制御をめざして」ウエスコ学術振興財団研究助成

    近藤 秀樹

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Awards

  • NIOC Prize

    2010   Nagoya International Orchid Congress 2010  

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    Country:Japan

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  • Encouragement Award

    2009   Nagoya International Orchid Congress 2009  

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    Country:Japan

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  • Outstanding reviewer

    2017.10   Virus Research  

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Research Projects

  • 絶対寄生菌ウイルスの研究プラットフォーム構築と生物防除への挑戦

    Grant number:23K18029  2023.06 - 2026.03

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

    近藤 秀樹, 鈴木 信弘

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    Grant amount:\6500000 ( Direct expense: \5000000 、 Indirect expense:\1500000 )

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  • 作物超個体における根圏RNAウイルス叢の実体解明とその生態学的役割

    Grant number:23H02214  2023.04 - 2027.03

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    近藤 秀樹, 久野 裕, 兵頭 究, 鈴木 信弘

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    Grant amount:\18590000 ( Direct expense: \14300000 、 Indirect expense:\4290000 )

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  • Frontiers in virus research using a model fungus Neurospora crassa

    Grant number:21K19086  2021.07 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    Kondo Hideki

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    Grant amount:\6500000 ( Direct expense: \5000000 、 Indirect expense:\1500000 )

    Fungal virology has made significant contributions to the field of basic virology. However, compared to the research fields of animal and plant viruses, fungi have limited effective model host systems to study virus-host interactions and advance virology research. In this study, we focused on the filamentous fungus Neurospora crassa, which is a model organism in genetics and molecular biology, and demonstrated its potential as a platform for studying the diversity of mycoviruses, virus-host interactions and host-antiviral mechanisms. Moreover, we were able to identify important host factors involved in fungal antiviral mechanisms, particularly in relation to transcriptional control mechanisms. Through our studies, we have confirmed that N. crassa is a promising model system for advancing both fundamental and applied research in fungal virology.

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  • 作物根圏におけるウイルス叢の多様性とその感染動態から紐解く生態的意義

    Grant number:20H02987  2020.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    近藤 秀樹, 久野 裕, 兵頭 究, 鈴木 信弘

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    Grant amount:\17680000 ( Direct expense: \13600000 、 Indirect expense:\4080000 )

    本課題では、ムギ類生存圏(特に根系)におけるウイルス叢の多様性・普遍性や動態を紐解き、さらにそれらの未知なる生態学的役割の一端に迫ることを目指している。以下に本年度の研究実績を示す。
    柱①根圏に存在する主要な菌類・昆虫ウイルス群の宿主探索:初年度に引き続き、ムギ類(オオムギ・コムギ)の地上部・根系、さらにアブラムシ、うどんこ病菌などのRNAseqデータを取得した。得られたデータセットはウイルス配列の確認作業や分子系統解析を進めている。さらに、北海道のコムギサンプルより見出された新規フレキシウイルス(WVQ)の解析の結果,当該圃場に三系統が存在し、ライムギにも感染できること、さらに土壌伝染性ウイルスの可能性が高いことを見出している。根系ウイルス叢のリザーバー探索については、本年度はオオムギ根のRNAseqデータに含まれる微生物リードの抽出と配列相同性解析により、微生物叢の概要を調査した。
    柱②根圏ウイルスの生物界を跨ぐ水平伝搬ポテンシャルと宿主への影響:昨年度より継続中のルテオウイルスなど新規植物ウイルスのゲノム解析を進めており、RT-PCRおよびRLM-RACE解析により全長ゲノムRNA配列を確認・決定した。また、圃場のうどんこ病菌のウイルスについては、今年度はdsRNAを鋳型にした全長cDNAのPCR増幅法の検討を進め、そのアンプリコン配列解析により未同定のゲノム末端配列の解析を進めている。
    柱③根圏ウイルス叢の年次変動とムギ類ウイルスの病原性の(再)評価:核酸抽出を伴わない簡易ウイルス診断法(RT-PCRをベースとする)の条件を設定し、一部圃場サンプル(葉)でルテオウイルス等の検出を進めている。現在、圃場オオムギサンプル(保存している過年度のを含む)について網羅的に検定すすめている。また、アブラムシ媒介性新規植物ウイルスについては、チャンバー内試験にて病原性評価を進めている。

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  • Virome of the phytopathogenic fungi: metagenomic analysis and the possible utilization

    Grant number:15K07312  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Kodno Hideki

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    Grant amount:\5070000 ( Direct expense: \3900000 、 Indirect expense:\1170000 )

    The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. However, virus discovery in obligate biotrophs such as powdery mildews (ascomycetes) and rust fungi (basidiomycetes) is still very limited. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least nine novel totivirus (genus Totivirus) in this fungus. Similar totvirus-like sequences are found in public transcriptome shotgun assembly (TSA) libraries of the bean rust fungi. Our data suggests that the horizontal transmission of ancestral totiviruses might have occurred across quite different fungal phyla, between powdery mildews and rust fungi and later evolved within the new host fungus separately.

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Class subject in charge

  • Seminar in Molecular Virology (2024academic year) Prophase  - その他

  • Seminar in Molecular Virology (2024academic year) Late  - その他

  • Seminar in Molecular Virology (2024academic year) Prophase  - その他

  • Seminar in Virology (2024academic year) Year-round  - その他

  • Applied Plant Virology (2024academic year) Late  - その他

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Social Activities

  • 悪いヤツだけじゃない!〜植物と関わるウイルスたち〜

    Role(s):Lecturer

    おかやま高梁川流域 倉敷市大学連携講座  2023.10.28

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    Type:Lecture

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Media Coverage

  • サイエンスBOX {知る} ウイルス、生物進化に一役 Newspaper, magazine

    読売新聞/ on line関西発  2017.6

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