研究者詳細 - 仁戸田　照彦

MISC - 仁戸田　照彦

分割表示 153 件中 141 - 153 件目／全件表示 >>

糸状菌の生産する細胞分裂阻害物質(-)-phenylahistinの培養生産

神崎浩, 福田隆雄, 仁戸田照彦

日本農芸化学会大会講演要旨集 2002 2002年

Agrobacterium属細菌の凝集促進活性を有する微生物細胞外多糖の性状解析

神崎浩, 新納愛, 仁戸田照彦

日本農芸化学会中四国支部講演会講演要旨集 4th (Web) 2002年

放線菌由来cyclo(Leu-Phe)脱水素酵素の精製と諸性質の検討

神崎浩, 森本篤史, 池田万里, 仁戸田照彦

日本農芸化学会中四国支部講演会講演要旨集 4th (Web) 2002年

1 脱水素型環状ジペプチド生合成酵素による生理活性物質生産(口頭発表の部)

神崎浩, 柳澤恵広, 赤澤和美, 池田万里, 森本篤史, 仁戸田照彦

天然有機化合物討論会講演要旨集 ( 43 ) 1 - 5 2001年9月

記述言語：日本語出版者・発行元：天然有機化合物討論会

Cyclic dipeptides (CDPs, diketopiperazines) and their derivatives are widely distributed in nature as secondary metabolites. Although some of dehydro-CDPs are known as cell cycle inhibitors, their effective syntheses have not been reported. We found that Streptomyces albulus KO23, an albonoursin-producing actinomycete, had a biosynthetic pathway from cyclo (Leu-Phe) to albonoursin, cyclo (ΔLeu-ΔPhe) by the fed-batch culture and the resting-cell experiments. And this enzyme activity was found to be effectively extracted in the cell-free extract of this actinomycete. This is the first report for the dehydrogenation of amino acid residues at α,β-positions in CDPs. Furthermore, this enzyme system enables us to synthesize several didehydro- and tetradehydro-CDPs from the corresponding CDPs. Among dehydro-CDPs prepared, the tetradehydro-CDPs exhibited cytotoxicity, while the didehydro-CDPs had no activity, indicating that dehydrogenation at α,β-positions of both amino acid residues in CDPs is required for cytotoxicity. Based on the above results, we speculated that a tetradehydro-CDP prepared from a didehydro-CDP exhibiting cytotoxicity might be a potent cytotoxic compound. Dehydrophenylahistin synthesized by this enzyme system from (-)-phenylahistin, which was recently reported to be a new cell cycle inhibitor, exhibited 1000 times higher inhibitory activity toward the first cleavage of sea urchin embryo than (-)-phenylahistin, and thus, would be a promising lead compound for antitumor agents.

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系状菌の生産する昆虫キチナーゼ阻害物質

日本農芸化学会大会(ポスタ-発表) 2000年

911 環状ジペプチド, cyclo(Gly-Leu)の微生物分解代謝に関与する新規酵素系

神崎浩, 三吉和之, 水田直樹, 仁戸田照彦

日本生物工学会大会講演要旨集 12 210 - 210 2000年