Updated on 2024/12/18

写真a

 
KAWAI Takashi
 
Organization
Faculty of Environmental, Life, Natural Science and Technology Associate Professor
Position
Associate Professor
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Degree

  • Master of Agriculture ( Kyoto University )

  • Doctor of Agriculture ( Kyoto University )

Research Interests

  • Pomology

  • Prunus

  • Peach

  • Fruit ripening

  • Softening trait

  • Inner disorder

  • Nondestructive evaluation

  • 3D point cloud analysis

  • Virus vector

Research Areas

  • Environmental Science/Agriculture Science / Horticultural science  / Pomology

Education

  • Kyoto University   農学研究科   農学専攻

    2013.4 - 2013.6

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    Country： Japan

    Notes： 博士課程

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  • Kyoto University   農学研究科   農学専攻

    2011.4 - 2013.3

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    Country： Japan

    Notes： 修士課程

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  • Kyoto University   農学部   資源生物科学科

    2007.4 - 2011.3

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    Country： Japan

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Research History

  • Okayama University   Graduate School of Environmental, Life, Natural Science and Technology   Associate Professor

    2023.4

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    Country：Japan

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  • Okayama University   Graduate School of Environmental and Life Science   Assistant Professor

    2017.4 - 2023.3

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    Country：Japan

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  • Kyoto University   Experimental Farm, Graduate School of Agriculture   Assistant Professor

    2013.6 - 2017.3

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    Country：Japan

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Professional Memberships

  • Japanese Society for Horticultural Science

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  • International Society for Horticultural Science

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Papers

  • Multi-year comparison of normal and split-pit peach quality Reviewed

    Takashi Kawai, Fumiya Matsumori, Akari Ikeda, Tami Ohashi, Go Inohara, Tomoya Ichioka, Anna Nishiguchi, Ryusei Mukai, Ken Hirano, Fumio Fukuda

    Acta Horticulturae   1404   1023 - 1030   2024.9

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：International Society for Horticultural Science (ISHS)  

    DOI： 10.17660/actahortic.2024.1404.140

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  • Effect of storage temperature and 1-MCP treatment on fruit ripening in Japanese apricot fruit (Prunus mume) Reviewed

    Chikara Yano, Tomoaki Kashiwamoto, Maqsood Muqadas, Kyohei Ohashi, Oscar Mitalo, Takashi Akagi, Fumio Fukuda, Takashi Kawai, Koichiro Ushijima, Yasutaka Kubo

    Acta Horticulturae   1404   717 - 724   2024.9

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：International Society for Horticultural Science (ISHS)  

    DOI： 10.17660/actahortic.2024.1404.98

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  • Degenerate oligonucleotide primer MIG‐seq: an effective PCR‐based method for high‐throughput genotyping Reviewed

    Kazusa Nishimura, Hiroyuki Kokaji, Ko Motoki, Akira Yamazaki, Kyoka Nagasaka, Takashi Mori, Rihito Takisawa, Yasuo Yasui, Takashi Kawai, Koichiro Ushijima, Masanori Yamasaki, Hiroki Saito, Ryohei Nakano, Tetsuya Nakazaki

    Plant Journal   118 ( 6 )   2296 - 2317   2024.3

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    SUMMARY

    Next‐generation sequencing (NGS) library construction often involves using restriction enzymes to decrease genome complexity, enabling versatile polymorphism detection in plants. However, plant leaves frequently contain impurities, such as polyphenols, necessitating DNA purification before enzymatic reactions. To overcome this problem, we developed a PCR‐based method for expeditious NGS library preparation, offering flexibility in number of detected polymorphisms. By substituting a segment of the simple sequence repeat sequence in the MIG‐seq primer set (MIG‐seq being a PCR method enabling library construction with low‐quality DNA) with degenerate oligonucleotides, we introduced variability in detectable polymorphisms across various crops. This innovation, named degenerate oligonucleotide primer MIG‐seq (dpMIG‐seq), enabled a streamlined protocol for constructing dpMIG‐seq libraries from unpurified DNA, which was implemented stably in several crop species, including fruit trees. Furthermore, dpMIG‐seq facilitated efficient lineage selection in wheat and enabled linkage map construction and quantitative trait loci analysis in tomato, rice, and soybean without necessitating DNA concentration adjustments. These findings underscore the potential of the dpMIG‐seq protocol for advancing genetic analyses across diverse plant species.

    DOI： 10.1111/tpj.16708

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  • Low Temperature Response and Effects of 1-MCP Treatment on Taiwanese and Japanese Cultivars of Japanese Apricot (Prunus mume Sieb. et Zucc.) during Postharvest Storage to Expand the Period of Fresh Fruit Supply Reviewed

    Tomoaki Kashiwamoto, Takashi Kawai, Takaaki Oe, Yasuhisa Tsuchida, Chikara Yano, Maqsood Muqadas, Takashi Akagi, Fumio Fukuda, Yasutaka Kubo, Koichiro Ushijima

    Horticultural Research (Japan)   22 ( 4 )   303 - 311   2023.10

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hrj.22.303

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  • Effect of Rootstock and Abscisic Acid (ABA) Treatment on Fruit Aroma Compounds of Grape ‘Shine Muscat’

    Hideo Yuki, Ryohei Iwai, Yoko Nakano, Takashi Kawai, Ken Hirano, Fumio Fukuda

    Bulletin of gunma agricultural technology center   ( 20 )   29 - 31   2023.3

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  • MIG-seq is an effective method for high-throughput genotyping in wheat (Triticum spp.) Reviewed

    Kazusa Nishimura, Ko Motoki, Akira Yamazaki, Rihito Takisawa, Yasuo Yasui, Takashi Kawai, Koichiro Ushijima, Ryohei Nakano, Tetsuya Nakazaki

    DNA Research   29 ( 2 )   1 - 13   2022.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    MIG-seq (Multiplexed inter-simple sequence repeats genotyping by sequencing) has been developed as a low cost genotyping technology, although the number of polymorphisms obtained is assumed to be minimal, resulting in the low application of this technique to analyses of agricultural plants. We applied MIG-seq to 12 plant species that include various crops and investigated the relationship between genome size and the number of bases that can be stably sequenced. The genome size and the number of loci, which can be sequenced by MIG-seq, are positively correlated. This is due to the linkage between genome size and the number of simple sequence repeats (SSRs) through the genome. The applicability of MIG-seq to population structure analysis, linkage mapping, and quantitative trait loci (QTL) analysis in wheat, which has a relatively large genome, was further evaluated. The results of population structure analysis for tetraploid wheat showed the differences among collection sites and subspecies, which agreed with previous findings. Additionally, in wheat biparental mapping populations, over 3,000 SNPs/indels with low deficiency were detected using MIG-seq, and the QTL analysis was able to detect recognized flowering-related genes. These results revealed the effectiveness of MIG-seq for genomic analysis of agricultural plants with large genomes, including wheat.

    DOI： 10.1093/dnares/dsac011

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    Other Link： https://academic.oup.com/dnaresearch/article-pdf/29/2/dsac011/43439343/dsac011.pdf

  • Control of Fruit Softening during Marine Transport Bound for North America in Three Astringent-type Persimmons Reviewed

    Takahiro Furuta, Takumi Otsuki, Rui Okada, Takashi Kawai, Fumio Fukuda, Koichiro Ushijima, Yasutaka Kubo, Ryohei Nakano

    Horticultural Research (Japan)   20 ( 4 )   455 - 461   2021.10

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hrj.20.455

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  • Effect of Split Pit on Maturation of ‘Shimizu Hakuto’ Peach on Trees Reviewed

    Takashi Kawai, Tomoya Ichioka, Akari Ikeda, Tami Ohashi, Go Inohara, Ken Hirano, Ryohei Nakano, Fumio Fukuda

    Horticulture Journal   90 ( 4 )   365 - 373   2021.10

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hortj.utd-285

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  • Effects of Ambient Temperature during Fruit Development on the Incidence of Reddish-pulp Disorder and Development of Functional Fruit Bag to Control Reddish-pulp Disorder in ‘Shimizu-hakuto’ Peach Reviewed

    Yuichiro Fujii, Kunihisa Morinaga, Takuya Murase, Rina Kishimoto, Maya Shimada, Akihisa Kitakoji, Masaya Ikeda, Toru Arakawa, Nariyoshi Emi, Manabu Teramura, Aritomo Araki, Tomoyuki Hino, Takashi Kawai, Ken Hirano, Fumio Fukuda

    Horticultural Research (Japan)   20 ( 2 )   189 - 197   2021.4

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hrj.20.189

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  • Combined signal sequence trap and macroarray analysis identifies genes associated with differential fruit softening characteristics during ripening in European and Chinese pears Reviewed

    Mercy W. Mwaniki, Oscar W. Mitalo, Eric G. Mworia, Willis O. Owino, Kyoko Hiwasa-Tanase, Jocelyn K.C. Rose, Koh Aoki, Tomoya Esumi, Takashi Kawai, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo

    Postharvest Biology and Technology   174   111436   2021.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.postharvbio.2020.111436

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  • Characterization of Fruit Enlargement and Ripening in Extremely Late Maturing Peach Reviewed

    Fumio Fukuda, Yuka Tamaki, Takashi Kawai, Koichiro Ushijima, Ken Hirano, Kenji Oda, Miyuki Hara, Yosuke Fukamatsu, Kunihisa Morinaga, Ryohei Nakano

    Horticultural Research (Japan)   20 ( 1 )   65 - 71   2021.1

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hrj.20.65

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  • Postharvest Properties of Ultra-Late Maturing Peach Cultivars and Their Attributions to Melting Flesh (M) Locus: Re-evaluation of M Locus in Association With Flesh Texture Reviewed

    Ryohei Nakano†, Takashi Kawai†(equal contribution), Yosuke Fukamatsu, Kagari Akita, Sakine Watanabe, Takahiro Asano, Daisuke Takata, Mamoru Sato, Fumio Fukuda, Koichiro Ushijima

    Frontiers in Plant Science   11   554158   2020.11

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    The postharvest properties of two ultra-late maturing peach cultivars, “Tobihaku” (TH) and “Daijumitsuto” (DJ), were investigated. Fruit were harvested at commercial maturity and held at 25°C. TH exhibited the characteristics of normal melting flesh (MF) peach, including rapid fruit softening associated with appropriate level of endogenous ethylene production In contrast, DJ did not soften at all during 3 weeks experimental period even though considerable ethylene production was observed. Fruit of TH and DJ were treated with 5,000 ppm of propylene, an ethylene analog, continuously for 7 days. TH softened rapidly whereas DJ maintained high flesh firmness in spite of an increase in endogenous ethylene production, suggesting that DJ but not TH lacked the ability to be softened in response to endogenous and exogenous ethylene/propylene. DNA-seq analysis showed that tandem endo-polygalacturonase (<italic>endoPG</italic>) genes located at <italic>melting flesh (M)</italic> locus, <italic>Pp-endoPGM</italic> (<italic>PGM</italic>), and <italic>Pp-endoPGF</italic> (<italic>PGF</italic>), were deleted in DJ. The <italic>endoPG</italic> genes at <italic>M</italic> locus are known to control flesh texture of peach fruit, and it was suggested that the non-softening property of DJ is due to the lack of <italic>endoPG</italic> genes. On the other hand, TH possessed an unidentified <italic>M</italic> haplotype that is involved in determination of MF phenotype. Structural identification of the unknown <italic>M</italic> haplotype, designated as <italic>M</italic><sup>0</sup>, through comparison with previously reported <italic>M</italic> haplotypes revealed distinct differences between <italic>PGM</italic> on <italic>M</italic><sup>0</sup> haplotype (<italic>PGM-M<sup>0</sup></italic>) and <italic>PGM</italic> on other haplotypes (<italic>PGM-M<sup>1</sup></italic>). Peach <italic>M</italic> haplotypes were classified into four main haplotypes: <italic>M</italic><sup>0</sup> with <italic>PGM-M<sup>0</sup></italic>; <italic>M</italic><sup>1</sup> with both <italic>PGM-M<sup>1</sup></italic> and <italic>PGF</italic>; <italic>M</italic><sup>2</sup> with <italic>PGM-M<sup>1</sup></italic>; and <italic>M</italic><sup>3</sup> lacking both <italic>PGM</italic> and <italic>PGF</italic>. Re-evaluation of <italic>M</italic> locus in association with MF/non-melting flesh (NMF) phenotypes in more than 400 accessions by using whole genome shotgun sequencing data on database and/or by PCR genotyping demonstrated that <italic>M</italic><sup>0</sup> haplotype was the common haplotype in MF accessions, and <italic>M</italic><sup>0</sup> and <italic>M</italic><sup>1</sup> haplotypes were dominant over <italic>M</italic><sup>2</sup> and <italic>M</italic><sup>3</sup> haplotypes and co-dominantly determined the MF trait. It was also assumed on the basis of structural comparison of <italic>M</italic> haplotypes among <italic>Prunus</italic> species that the ancestral haplotype of <italic>M</italic><sup>0</sup> diverged from those of the other haplotypes before the speciation of <italic>Prunus persica</italic>.

    DOI： 10.3389/fpls.2020.554158

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  • Control of Fruit Softening during Marine Transport Bound for Southeast Asia in 'Nakatani-Wase' Persimmon Reviewed

    Takahiro Furuta, Manabu Wanaka, Shohei Kumamoto, Hirokazu Ikenaga, Reiko Nishime, Takashi Kawai, Yosuke Fukamatsu, Fumio Fukuda, Yasutaka Kubo, Ryohei Nakano

    Horticultural Research (Japan)   19 ( 3 )   299 - 307   2020.7

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE  

    <p>The combined effect of 1-methylcyclopropene (1-MCP) application on CO2 exposure for astringency removal and packaging techniques on suppressing fruit softening during marine transport bound for southeast Asia was tested in early-maturing persimmon, 'Nakatani-Wase' fruit. In tests while simulating marine transport, 1-MCP application in combination with all packaging techniques tested in this experiment effectively suppressed the softening of fruit. Under conditions simulating the shelf-life after arrival, the combination of 1-MCP application and modified atmosphere (MA) packaging at both individual fruit and carton box levels suppressed fruit softening for 7 days at room temperature and for more than 10 days at 5°C. On the other hand, fruit packaged in normal carton boxes or boxes coated with water-impervious material softened immediately after arrival at room temperature and after 5 days at 5°C. Practical transport tests involving marine transport bound for Singapore confirmed that the combination of 1-MCP application and MA packaging in carton boxes led to the complete absence of softened fruit upon arrival in Singapore and reduced it to just 13.3% 6 days after transfer to room temperature. These results indicate that the combination of 1-MCP application, MA packaging, and a reefer container for temperature control is a practical quality control technique for marine transport of 'Nakatani-Wase' fruit bound for southeast Asia.</p>

    DOI： 10.2503/hrj.19.299

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  • Effects of fungicide application and addition of a sticker adjuvant to the fungicide on the occurrence of fruit rot in Satsuma mandarin after refrigerated maritime transportation from Japan to Singapore Reviewed

    Takeda Tomoaki, Eto Kayou, Okamuro Mieko, Inuma Takashi, Nakano Ryuhei, Fukuda Fumio, Kawai Takashi, Fukamatsu Yousuke, Nishime Reiko

    Annual Report of The Kansai Plant Protection Society   62   113 - 119   2020.5

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：The Kansai Plant Protection Society  

    <p>Several experiments were conducted to establish an efficient system for controlling fruit rot when exporting Satsuma mandarin to Singapore. A total of 110 isolates of Penicillium digitatum obtained from 44 orchards in Wakayama Prefecture were assayed for sensitivity to thiophanate-methyl, benomyl, and iminoctadine triacetate. Of these isolates, 8.2 percent showed resistance to thiophanate-methyl and benomyl, and all isolates showed sensitivity toward iminoctadine triacetate. These three fungicides seemed to be effective. In a refrigerated maritime transport experiment in which Satsuma mandarin was taken from Japan to Singapore, spraying thiophanate-methyl wettable powder before harvesting suppressed the occurrence of fruit rot 19 days after devanning to about 41% of that of an untreated load. Addition of a paraffinic sticker adjuvant to thiophanate-methyl wettable powder or iminoctadine triacetate liquid decreased the rate of occurrence of fruit rot in an inoculation test of P. digitatum following artificial precipitation and reduced the occurrence of fruit rot 14 days after devanning in a refrigerated maritime transport experiment in which Satsuma mandarin was taken to Singapore. Adding a paraffinic sticker adjuvant to fungicides sprayed before harvesting was considered to improve the efficacy of fruit rot control after refrigerated maritime transportation of Satsuma mandarin from Japan to Singapore.</p>

    DOI： 10.4165/kapps.62.113

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  • Characterization of Ethylene Production and Fruit Softening in ‘Tosui’ Peach Reviewed

    Takashi Kawai, Kagari Akita, Sakine Watanabe, Yosuke Fukamatsu, Daisuke Takata, Mamoru Sato, Koichiro Ushijima, Fumio Fukuda, Ryohei Nakano

    Horticultural Research (Japan)   19 ( 1 )   61 - 67   2020.1

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    Authorship：Lead author   Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hrj.19.61

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  • モモ‘紅清水’における赤肉果の音響振動法による非破壊判別技術の検討

    Fumio Fukuda, Takanori Kondo, Takashi Kawai, Ken Hirano, Kunihisa Morinaga

    Bulletin of the Field Science Center, Faculty of Agriculture, Okayama University   41   9 - 13   2019.4

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  • Nondestructive Detection of Split-pit Peach Fruit on Trees with an Acoustic Vibration Method Reviewed

    Takashi Kawai, Fumiya Matsumori, Hidemi Akimoto, Naoki Sakurai, Ken Hirano, Ryohei Nakano, Fumio Fukuda

    Horticulture Journal   87 ( 4 )   499 - 507   2018.10

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hortj.utd-012

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  • Nondestructive Detection of Split Pit in Peaches Using an Acoustic Vibration Method Reviewed

    Ryohei Nakano, Hidemi Akimoto, Fumio Fukuda, Takashi Kawai, Koichiro Ushijima, Yosuke Fukamatsu, Yasutaka Kubo, Yuichiro Fujii, Ken Hirano, Kunihisa Morinaga, Naoki Sakurai

    Horticulture Journal   87 ( 2 )   281 - 287   2018.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hortj.okd-094

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  • モモ‘清水白桃’における開花日を考慮した摘花法が果実品質に及ぼす影響

    Fumio Fukuda, Takanori Kondo, Kazufumi Horinishi, Takashi Kawai, Ken Hirano, Kunihisa Morinaga

    Bulletin of the Field Science Center, Faculty of Agriculture, Okayama University   40   9 - 13   2018.4

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  • Identification of QTLs controlling chilling and heat requirements for dormancy release and bud break in Japanese apricot (Prunus mume) Reviewed

    Yuto Kitamura, Tsuyoshi Habu, Hisayo Yamane, Soichiro Nishiyama, Kei Kajita, Takuya Sobue, Takashi Kawai, Koji Numaguchi, Tetsuya Nakazaki, Akira Kitajima, Ryutaro Tao

    Tree Genetics & Genomes   14   33   2018.3

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER HEIDELBERG  

    Bud dormancy is an important developmental stage affecting blooming date and leafing date (LD) in Japanese apricot (Prunus mume), but the genetic factors controlling the chilling requirement (CR) and heat requirement (HR) for dormancy release and bud burst time remain to be elucidated. Here, a quantitative trait locus (QTL) analysis using two F-1 segregating populations was conducted to identify loci affecting these traits. The genotyping-by-sequencing technique was used to construct two high-density genetic maps, one for NKSC, a population derived from high-chill 'Nanko' crossed with low-chill 'SC', covering 660.2 cM with 408 markers, and one for NINK, a population derived from low-chill 'Ellching' crossed with 'Nanko', covering 1314.2 cM with 718 markers. We observed four traits: CR and HR for dormancy release, blooming date, and LD over several years. To identify the QTL controlling the downregulation of DORMANCY-ASSOCIATED MADS-box6 (PmDAM6) in January's leaf buds, in which PmDAM6 could act as a dose-dependent inhibitor of bud break, its transcript levels in leaf buds were determined. All traits segregated in the analyzed seasons in both populations. For leaf bud dormancy, CR and LD were highly correlated across years and traits in the NKSC population, while HR, LD, and PmDAM6 expression were highly correlated in the NINK population. The QTL analyses localized the significant QTLs controlling leaf bud CR and HR, LD, and PmDAM6 expression in leaf buds to a region in linkage group 4, which suggests that this locus controls dormancy release, bud break, and PmDAM6's downregulation in Japanese apricot leaf buds.

    DOI： 10.1007/s11295-018-1243-3

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    Other Link： http://link.springer.com/content/pdf/10.1007/s11295-018-1243-3.pdf

  • A male determinant gene in diploid dioecious Diospyros, OGI, is required for male flower production in monoecious individuals of Oriental persimmon (D. kaki) Reviewed

    Takashi Akagi, Takashi Kawai, Ryutaro Tao

    Scientia Horticulturae   213   243 - 251   2016.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.scienta.2016.10.046

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  • Epigenetic Regulation of the Sex Determination Gene MeGI in Polyploid Persimmon Reviewed

    Takashi Akagi, Isabelle M. Henry, Takashi Kawai, Luca Comai, Ryutaro Tao

    Plant Cell   28 ( 12 )   2905 - 2915   2016.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1105/tpc.16.00532

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  • Differences in Physiological Characteristics and Gene Expression Levels in Fruits between Japanese Persimmon (Diospyros kaki Thunb.) ‘Hiratanenashi’ and Its Small Fruit Mutant ‘Totsutanenashi’ Reviewed

    Tsuyoshi Habu, Hisayo Yamane, Ippei Naito, Soichiro Nishiyama, Ayumi Nonaka, Takashi Kawai, Hisashi Yamada, Ryutaro Tao

    Horticulture Journal   85 ( 4 )   306 - 314   2016.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hortj.mi-119

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  • Cell wall related gene expression during secondary physiological fruit drop in ponkan (Citrus reticulata Blanco) and hyuganatsu (C. tamuranahort. ex Tanaka) Reviewed

    Xi Li, Akira Kitajima, Keisuke Katsura, Hiroki Saito, Sota Koeda, Rihito Takisawa, Takashi Kawai, Tetsuya Nakazaki, Tokuro Shimizu

    Acta Horticulturae   1135   47 - 52   2016.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：International Society for Horticultural Science (ISHS)  

    DOI： 10.17660/actahortic.2016.1135.6

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  • Virus-induced gene silencing in various Prunus species with the Apple latent spherical virus vector Reviewed

    Takashi Kawai, Ayako Gonoi, Michiya Nitta, Noriko Yamagishi, Nobuyuki Yoshikawa, Ryutaro Tao

    Scientia Horticulturae   199   103 - 113   2016.2

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.scienta.2015.12.031

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  • Development of Molecular Markers Associated with Sexuality in Diospyros lotus L. and Their Application in D-kaki Thunb Reviewed

    Takashi Akagi, Kei Kajita, Takanori Kibe, Haruka Morimura, Tomoyuki Tsujimoto, Soichiro Nishiyama, Takashi Kawai, Hisayo Yamane, Ryutaro Tao

    Journal of the Japanese Society for Horticultural Science   83 ( 3 )   214 - 221   2014.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPAN SOC HORTICULTURAL SCI  

    Sexuality of crops affects both cultivation and breeding systems. Cultivated persimmon (Diospyros kaki Thunb) has a morphologically well-characterized polygamous or gyonodioecious sexual system. However, the genetic basis of sexuality in D. kaki has yet to be characterized. Here, we used dioecious D. lotus L., a diploid wild relative species to hexaploid or nonaploid D. kaki, as a model to clarify the genetic basis of sexuality in Diospyros and to develop molecular markers associated with the sexuality of individuals. Using 62 F-1 offspring segregated into distinct male/female phenotypes, we found two amplified fragment-length polymorphism markers, DlSx-AF4 and DlSx-AF7, which cosegregated with maleness. This could suggest that the sexuality of D. lotus is controlled by a single gene/haploblock, and the male is dominant over the female. Thus, D. lotus's sexuality can be described as the heterogametic male type, the ICY-type, as reported for most other dioecious plant species. For unknown reasons, segregation of the phenotype of a sequence-characterized amplified region marker developed from DlSx-AF4 (DlSx-AF4S) and/or the male/female phenotype in two different crosses in D. lotus showed an apparent bias towards femaleness and better fitted 1:2 than 1:1, which is the theoretical segregation for a single genetic locus or haploblock in diploid D. lotus. DlSx-AF4S could distinguish D. kaki cultivars with female and male flowers from cultivars with only female flowers, strongly indicating that the same genetic system controls D. kaki's sexuality and that DlSx-AF4S could be used as a genetic marker for sexuality in D. kaki breeding programs.

    DOI： 10.2503/jjshs1.CH-109

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  • Virus-induced Gene Silencing in Apricot (Prunus armeniaca L.) and Japanese Apricot (P. mume Siebold & Zucc.) with the Apple Latent Spherical Virus Vector System Reviewed

    Takashi Kawai, Ayako Gonoi, Michiya Nitta, Masanori Kaido, Noriko Yamagishi, Nobuyuki Yoshikawa, Ryutaro Tao

    Journal of the Japanese Society for Horticultural Science   83 ( 1 )   23 - 31   2014.1

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/jjshs1.ch-091

    Web of Science

    CiNii Article

    J-GLOBAL

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  • Small-fruit mutant of 'hiratane-nashi' persimmon (Diospyros kaki Thunb.) Reviewed

    Ryutaro Tao, Takashi Kawai, Hisayo Yamane

    Acta Horticulturae   996   127 - 132   2013.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：International Society for Horticultural Science (ISHS)  

    DOI： 10.17660/actahortic.2013.996.15

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Books

  • 青果物のおいしさの科学（3編1章3節）モモ

    福田文夫, 河井 崇（3編1章3節: 303-309）

    株式会社エヌ・ティー・エス  2024.11 

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  • 果実日本（第79巻第1号）モモにおける音響振動法による果実硬度の推定と内部障害発生の把握

    福田文夫, 河井 崇, 中野龍平（第79巻第1号: 72-75）

    日本園芸農業組合連合会（日園連）  2024.1 

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  • 果実日本（第77巻第7号）高性能冷蔵コンテナを利用したシャインマスカット長期貯蔵

    福田文夫, 河井 崇, 中野龍平（第77巻第7号: 78-81）

    日本園芸農業組合連合会（日園連）  2022.7 

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  • 農業技術大系・果樹編（第6巻追録第35号）音響振動装置を用いた非破壊内部診断

    福田文夫, 河井 崇, 中野龍平（第6巻追録第35号: 技56の1の2-6）

    農山漁村文化協会（農文協）  2020.6 

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  • 青果物の鮮度評価・保持技術（8章2節3項）海上輸送によるモモとブドウのシンガポール輸出

    中野龍平, 深松陽介, 福田文夫, 河井 崇（8章2節3項: 329-335）

    株式会社エヌ・ティー・エス  2019.12 

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  • 青果物の鮮度評価・保持技術（7章3節7項）高性能冷蔵コンテナ利用低温貯蔵技術

    福田文夫, 河井 崇, 深松陽介, 中野龍平（7章3節7項: 249-256）

    株式会社エヌ・ティー・エス  2019.12 

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  • 青果物の鮮度評価・保持技術（6章8節）音響振動法によるモモの内部障害の非破壊判別

    河井 崇, 福田文夫, 中野龍平（6章8節: 177-181）

    株式会社エヌ・ティー・エス  2019.12 

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  • 農業技術大系・果樹編（第6巻追録第34号）果実の貯蔵，鮮度保持

    中野龍平, 福田文夫, 河井 崇（第6巻追録第34号: 技56の2-6）

    農山漁村文化協会（農文協）  2019.6 

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  • 果樹（第73巻5号）よくわかる果樹用語解説 X. ブドウおよびモモの硬核期

    河井 崇（73巻5号: 22-26）

    全国農業協同組合連合会 岡山県本部（JA全農おかやま）  2019.5 

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MISC

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Presentations

  • 樹体3Dモデル研究の果樹栽培への応用

    Takashi Kawai

    2024.12 

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    Event date： 2024.12.14

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Histomorphological study in seedless mechanism and effects of timing of single GA treatment on fruit quality in 'HohoemiE grape

    Fumio Fukuda, Kana Yamamoto, Kayu Moriyasu, Kanako Tominaga, Takashi Kawai, Shogo Nogami, Ken Hirano

    2024.11 

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    Event date： 2024.11.3 - 2024.11.5

    Language：Japanese   Presentation type：Poster presentation  

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  • Changes in the traits associated with fruit and seed maturation during fruit development in Taiwanese and Japanese cultivars of Japanese apricot

    Tomoaki Kashiwamoto, Hikaru Tajima, So Michiue, Takashi Kawai, Yasutaka Kubo, Koichiro Ushijima

    2024.11 

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    Event date： 2024.11.3 - 2024.11.5

    Language：Japanese   Presentation type：Poster presentation  

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  • 日本のモモ栽培品種育成における4番染色体のハプロタイプの遺伝

    Misa Iwamoto, Mai Minamikawa, Ryohei Nakano, Daisuke Takata, Takashi Kawai, Fumio Fukuda, Yujiro Unoki, Kenji Oda, Koichiro Ushijima

    2024.9 

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    Event date： 2024.9.19 - 2024.9.20

    Language：Japanese  

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  • Fruit enlargement and cell growth of ‘Fukushimadaigakuichigo’ peach

    Daisuke Takata, Miyu Iizuka, Takashi Kawai, Fumio Fukuda, Koichiro Ushijima, Ryohei Nakano

    2024.8 

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    Event date： 2024.8.26 - 2024.8.27

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Effects of salicylic acid spraying to on-tree fruit on the harvest time and quality in 'Benishimizu' peach

    Fumio Fukuda, Masaya Hasegawa, Sae Matsuda, Shotaro Kino, Yuri Shimatani, Takashi Kawai, Ken Hirano

    2024.7 

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    Event date： 2024.7.20

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Dynamics of transcriptome profiling during fruit development of Taiwanese and Japanese cultivars in Japanese apricot

    Tomoaki Kashiwamoto, Takashi Kawai, Takaaki Oe, Koji Numaguchi, Yuto Kitamura, Yasutaka Kubo, Takashi Akagi, Fumio Fukuda, Koichiro Ushijima

    2024.3 

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    Event date： 2024.3.23 - 2024.3.24

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Estimation of the amount of pruning in peach using 3D point cloud data

    Takashi Kawai, Haruki Ono, Kotoko Nishii, Kazutoshi Hamada, Michisuke Yamamoto, Daisuke Takata, Fumio Fukuda

    2024.3 

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    Event date： 2024.3.23 - 2024.3.24

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Comparison of fruit softening traits among ultra-late maturing peach ‘Fuyumomogatari’ and its F1 individuals focusing on melting flesh (M) locus (II)

    Masaharu Abe, Yuzuki Hayashi, Yuki Kosaka, Ryohei Nakano, Koichiro Ushijima, Fumio Fukuda, Takashi Kawai

    2024.3 

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    Event date： 2024.3.23 - 2024.3.24

    Language：Japanese   Presentation type：Poster presentation  

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  • Presumption of fruit maturity and prediction of harvest time using acoustic vibration method in late ripening 'Hakuo' peach

    Ryusei Mukai, Kakeru Hatakeyama, Go Inohara, Takashi Kawai, Ken Hirano, Fumio Fukuda

    2024.3 

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    Event date： 2024.3.23 - 2024.3.24

    Language：Japanese   Presentation type：Poster presentation  

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  • 三次元点群解析によるモモの果実・樹体情報の取得

    Takashi Kawai

    2023.11 

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    Event date： 2023.11.15

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Multi-year comparison of normal and split-pit peach quality

    Takashi Kawai, Fumiya Matsumori, Akari Ikeda, Tami Ohashi, Go Inohara, Tomoya Ichioka, Anna Nishiguchi, Ryusei Mukai, Ken Hirano, Fumio Fukuda

    4th Asian Horticultural Congress  2023.8 

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    Event date： 2023.8.28 - 2023.8.31

    Language：English   Presentation type：Poster presentation  

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  • Nondestructive evaluation of berry softening by an acoustic vibration method in three grape cultivars

    Fumio Fukuda, Ryusuke Fukui, Kayu Moriyasu, Takashi Kawai, Minako Kawamura, Itsuki Takashima, Naoki Sakurai, Ken Hirano

    4th Asian Horticultural Congress  2023.8 

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    Event date： 2023.8.28 - 2023.8.31

    Language：English   Presentation type：Poster presentation  

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  • Comparative genomic analysis between Japanese peach cultivar ‘Akatsuki’ and its bud sport mutants with diverse maturity dates

    Masaru Amamori, Koichiro Ushijima, Ryohei Nakano, Fumio Fukuda, Takashi Kawai, Kazusa Nishimura, Ko Motoki, Kyoka Nagasaka, Hiromo Inoue, Yu Iwahashi, Tetsuya Nakazaki

    4th Asian Horticultural Congress  2023.8 

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    Event date： 2023.8.28 - 2023.8.31

    Language：English   Presentation type：Oral presentation (general)  

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  • Effect of storage temperature and 1-MCP treatment on fruit ripening in Japanese apricot fruit (Prunus mume)

    Chikara Yano, Tomoaki Kashiwamoto, Maqsood Muqadas, Kyohei Ohashi, Oscar Mitalo, Takashi Akagi, Fumio Fukuda, Takashi Kawai, Koichiro Ushijima, Yasutaka Kubo

    4th Asian Horticultural Congress  2023.8 

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    Event date： 2023.8.28 - 2023.8.31

    Language：English   Presentation type：Poster presentation  

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  • Characteristics in skilled grower’s movement of line of sight during thinning of flower bud or fruit in peach growing

    Fumio Fukuda, Sae Matsuda, Takashi Kawai, Ken Hirano, Hidetake Uwano

    2023.7 

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    Event date： 2023.7.22

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 三次元点群データを用いた果樹類の樹体・果実情報の取得

    Takashi Kawai

    2023.6 

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    Event date： 2023.6.30

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Construction of a 3D model for the relationship between fruit position in the canopy and quality in peach using 3D point cloud data

    Takashi Kawai, Yuki Kosaka, Yuzuki Hayashi, Masaharu Abe, Michisuke Yamamoto, Atsushi Sekimoto, Kazutoshi Hamada, Fumio Fukuda, Daisuke Takata

    2023.3 

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    Event date： 2023.3.15 - 2023.3.22

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Low temperature resistance and effects of 1-MCP treatments on quality preservation during postharvest storage in Japanese apricot fruit of Taiwanese cultivars

    Tomoaki Kashiwamoto, Takashi Kawai, Takaaki Oe, Chikara Yano, Maqsood Muqadas, Takashi Akagi, Fumio Fukuda, Yasutaka Kubo, Koichiro Ushijima

    2023.3 

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    Event date： 2023.3.15 - 2023.3.22

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Low-temperature storage of ultra-late maturing peach ‘Fuyumomogatari’ towards the supply of fresh peach fruit during the end-of-the-year season

    Masaharu Abe, Takashi Kawai, Yuki Kosaka, Yuzuki Hayashi, Hikaru Kawasaki, Fumio Fukuda

    2023.3 

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    Event date： 2023.3.15 - 2023.3.22

    Language：Japanese   Presentation type：Poster presentation  

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  • Comparison of fruit softening traits among ultra-late maturing peach ‘Fuyumomogatari’ and its F1 individuals focusing on melting flesh (M) locus

    Yuzuki Hayashi, Takashi Kawai, Yuki Kosaka, Yuka Nasu, Hikaru Kawasaki, Masaharu Abe, Ryohei Nakano, Koichiro Ushijima, Fumio Fukuda

    2023.3 

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    Event date： 2023.3.15 - 2023.3.22

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Application of an acoustic vibration tester for harvesting of 'Hakurei' peach

    Ryusei Mukai, Anna Nishiguchi, Go Inohara, Ryosuke Yoshimura, Takashi Kawai, Ken Hirano, Fumio Fukuda

    2022.10 

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    Event date： 2022.10.1

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Development of "degenerate oligonucleotide primer MIG-seq", an improved method of MIG-seq, and its application to crop genetic analysis

    Kazusa Nishimura, Hiroyuki Kokaji, Ko Motoki, Akira Yamazaki, Kyoka Nagasaka, Rihito Takisawa, Yasuo Yasui, Takashi Kawai, Koichiro Ushijima, Masanori Yamasaki, Hiroki Saito, Ryohei Nakano, Tetsuya Nakazaki

    2022.9 

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    Event date： 2022.9.23 - 2022.9.25

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Application of MIG-seq for genetic analysis and selection of NILs in wheat

    Kazusa Nishimura, Ko Motoki, Akira Yamazaki, Rihito Takisawa, Yasuo Yasui, Takashi Kawai, Koichiro Ushijima, Ryohei Nakano, Tetsuya Nakazaki

    2nd International Wheat Congress  2022.9 

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    Event date： 2022.9.11 - 2022.9.15

    Language：English   Presentation type：Poster presentation  

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  • Fruit development and ethylene production in Japanese and Taiwanese cultivars of Japanese apricot

    Tomoaki Kashiwamoto, Takashi Kawai, Koji Numaguchi, Takaaki Oe, Takashi Akagi, Yasutaka Kubo, Fumio Fukuda, Koichiro Ushijima

    2022.9 

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    Event date： 2022.9.7 - 2022.9.13

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Effect of salicylic acid treatment on infection of leaf curl disease in peach

    Takashi Kawai, Fumio Fukuda

    2022.9 

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    Event date： 2022.9.7 - 2022.9.13

    Language：Japanese   Presentation type：Poster presentation  

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  • Effect of on-tree judgement of split pit fruit and thinning by an acoustic vibration instrument on the reduction of harvest losses in 'Shimizu Hakuto' peach

    Go Inohara, Ryosuke Yoshimura, Anna Nishiguchi, Ryusei Mukai, Takashi Kawai, Ken Hirano, Fumio Fukuda

    2022.9 

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    Event date： 2022.9.7 - 2022.9.13

    Language：Japanese   Presentation type：Poster presentation  

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  • Characteristics of "Water-soaked brown flesh" disorder and morphological change of immature flesh in 'Hakuho' peach

    Ryosuke Yoshimura, Go Inohara, Anna Nishiguchi, Ryusei Mukai, Takashi Kawai, Ken Hirano, Fumio Fukuda

    2022.3 

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    Event date： 2022.3.21 - 2022.3.22

    Language：Japanese   Presentation type：Poster presentation  

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  • Comparison of berry characteristics between 'Hohoemi' and 'Shine Muscut' grape

    Ken Hirano, Fumio Fukuda, Takashi Kawai, Shogo Nogami, Tomio Shimura

    2022.3 

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    Event date： 2022.3.20 - 2022.3.21

    Language：Japanese   Presentation type：Poster presentation  

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  • The chromosome level reference genome assembly for Japanese peach cultivar 'Akatsuki' and extremely late maturing peach 'Fuyumomogatari'

    Koichiro Ushijima, Takashi Kawai, Takahiro Asano, Fumio Fukuda, Ryohei Nakano

    2022.3 

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    Event date： 2022.3.20 - 2022.3.21

    Language：Japanese   Presentation type：Poster presentation  

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  • Divergent expression among polygalacturonase (PG) genes in melting flesh (M) locus in peach

    Takashi Kawai, Sakine Watanabe, Yuka Nasu, Yuki Yoshida, Daisuke Takata, Mamoru Sato, Tomoyuki Hino, Yuichiro Fujii, Fumio Fukuda, Ryohei Nakano, Koichiro Ushijima

    2022.3 

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    Event date： 2022.3.20 - 2022.3.21

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Continuous fruit firmness measurement of berries in 'Shine Muscat' grape using an acoustic vibration method

    Fumio Fukuda, Takashi Kawai, Takanori Kondo, Tomiko Sakai, Ken Hirano, Itsuki Takashima, Naoki Sakurai, Ryohei Nakano

    2022.3 

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    Event date： 2022.3.20 - 2022.3.21

    Language：Japanese   Presentation type：Poster presentation  

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  • Application of MIG-seq to crop genomics: High-throughput genotyping of wheat is realized by MIG-seq

    Kazusa Nishimura, Ko Motoki, Akira Yamazaki, Yasuo Yasui, Rihito Takisawa, Takashi Kawai, Koichiro Ushijima, Ryohei Nakano, Tetsuya Nakazaki

    2021.9 

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    Event date： 2021.9.23 - 2021.9.25

    Language：Japanese   Presentation type：Poster presentation  

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  • Changes in flesh cell number and size during fruit development in ultra-late maturing peach ‘Fuyumomogatari’

    Yuki Kosaka, Takashi Kawai, Shinya Tahara, Fumio Fukuda

    2021.9 

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    Event date： 2021.9.11 - 2021.9.12

    Language：Japanese   Presentation type：Poster presentation  

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  • Ethylene production and fruit softening in peaches with different flesh texture during cold storage

    Sakine Watanabe, Takashi Kawai, Yuka Nasu, Yuki Yoshida, Daisuke Takata, Mamoru Sato, Koichiro Ushijima, Yasutaka Kubo, Ryohei Nakano, Fumio Fukuda

    2021.9 

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    Event date： 2021.9.11 - 2021.9.12

    Language：Japanese   Presentation type：Poster presentation  

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  • Relationship between fruit softening traits and two loci regulating flesh texture in late maturing peach cultivars

    Yuka Nasu, Takashi Kawai, Sakine Watanabe, Yuki Yoshida, Daisuke Takata, Mamoru Sato, Koichiro Ushijima, Ryohei Nakano, Fumio Fukuda

    2021.9 

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    Event date： 2021.9.11 - 2021.9.12

    Language：Japanese   Presentation type：Poster presentation  

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  • A candidate gene responsible for susceptibility to the host-specific toxin of Alternaria alternata pathotype peach

    Takahiro Asano, Takashi Kawai, Yujiro Unoki, Seisuke Hihara, Kenta Shirasawa, Takashi Akagi, Yasutaka Kubo, Daisuke Takata, Ryohei Nakano, Fumio Fukuda, Mikihiro Yamamoto, Koichiro Ushijima

    2021.9 

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    Event date： 2021.9.11 - 2021.9.12

    Language：Japanese   Presentation type：Poster presentation  

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  • 'Fukushimadaigakuichigo', a new bud sport of peach 'Beni-hakuto'

    Mamoru Sato, Daisuke Takata, Takayuki Tamura, Takashi Kawai, Koichiro Ushijima, Fumio Fukuda, Ryohei Nakano

    2021.9 

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    Event date： 2021.9.11 - 2021.9.12

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • LOH (loss of heterozygosity) hot spots detected on chromosome 4 in a bud sport of 'Beni-hakuto' peach

    Yuki Taniguchi, Koichiro Ushijima, Takashi Kawai, Takayuki Tamura, Fumio Fukuda, Daisuke Takata, Mamoru Sato, Kazusa Nishimura, Eri Maai, Ko Motoki, Akira Yamazaki, Tetsuya Nakazaki, Ryohei Nakano

    2021.9 

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    Event date： 2021.9.11 - 2021.9.12

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Availability of an acoustic vibration method with an integrated sensor on the estimation of split-pit occurrence and fruit maturity in peaches

    Fumio Fukuda, Takashi Kawai, Go Inohara, Anna Nishiguchi, Ryosuke Yoshimura, Itsuki Takashima, Ken Hirano, Naoki Sakurai

    2021.3 

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    Event date： 2021.3.27 - 2021.3.28

    Language：Japanese   Presentation type：Poster presentation  

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  • Evolution of Melting Flesh (M) locus controlling the peach flesh texture in Prunus genus

    Koichiro Ushijima, Takashi Kawai, Kagari Akita, Sakine Watanabe, Takahiro Asano, Daisuke Takata, Mamoru Sato, Fumio Fukuda, Ryohei Nakano

    2021.3 

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    Event date： 2021.3.27 - 2021.3.28

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Haplotyping of melting flesh (M) locus in major peach cultivars grown in Japan

    Takashi Kawai, Koichiro Ushijima, Kagari Akita, Sakine Watanabe, Takahiro Asano, Daisuke Takata, Mamoru Sato, Fumio Fukuda, Ryohei Nakano

    2021.3 

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    Event date： 2021.3.27 - 2021.3.28

    Language：Japanese   Presentation type：Poster presentation  

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  • Effect of split pit on maturity of ‘Shimizu Hakuto’ peach

    Tomoya ichioka, Takashi Kawai, Akari Ikeda, Tami Ohashi, Go inohara, Ryohei Nakano, Fumio Fukuda

    2021.3 

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    Event date： 2021.3.27 - 2021.3.28

    Language：Japanese   Presentation type：Poster presentation  

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  • Nondestructive detection of inner disorders in peach fruit using an acoustic vibration method

    Takashi Kawai

    Can Tho University-Okayama University Joint Seminar  2020.12 

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    Event date： 2020.12.11

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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  • Postharvest properties in ultra-late maturing peaches and their attributions to M locus: novel M haplotypes and re-evaluation of M locus-effect on flesh texture

    Ryohei Nakano, Takashi Kawai, Takahiro Asano, Kagari Akita, Sakine Watanabe, Daisuke Takata, Mamoru Sato, Fumio Fukuda, Koichiro ushijima

    2020.10 

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    Event date： 2020.10.10 - 2020.10.11

    Language：Japanese   Presentation type：Poster presentation  

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  • Estimation of locus region associated with susceptibility to black spot of peach

    Takahiro Asano, Takashi Kawai, Yujiro Unoki, Seisuke Hihara, Daisuke Takata, Kenta Shirasawa, Takashi Akagi, Fumio Fukuda, Ryohei Nakano, Yasutaka Kubo, Mikihiro Yamamoto, Koichiro Ushijima

    2020.10 

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    Event date： 2020.10.10 - 2020.10.11

    Language：Japanese   Presentation type：Poster presentation  

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  • Inheritance of ultra-late maturing trait of ‘Fuyumomogatari’ peach in F1 progeny

    Takashi Kawai, Koichiro Ushijima, Haruna Miyake, Mei Kusaka, Yosuke Fukamatsu, Miyuki Hara, Yujiro Unoki, Seisuke Hihara, Ryohei Nakano, Kenji Oda, Fumio Fumio

    2020.3 

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    Event date： 2020.3.21 - 2020.3.22

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Effects of the maturity level on a success storage around 0°C and volatile components after the storage in the late-ripening peach ‘Hakuro’

    Ryohei iwai, Takashi Kawai, Tomoyuki Hino, Yuichiro Fujii, Ivan Galis, Ken Hirano, Ryohei Nakano, Fumio Fukuda

    2020.3 

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    Event date： 2020.3.21 - 2020.3.22

    Language：Japanese   Presentation type：Poster presentation  

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  • Susceptibility to black spot of peach in peach genetic resources and hybrid populations

    Takahiro Asano, Takashi Kawai, Natsuki Ito, Yuri Ashida, Ayaka Ijima, Yujiro Unoki, Daisuke Takata, Ryohei Nakano, Fumio Fukuda, Yasutaka Kubo, Takashi Akagi, Mikihiro Yamamoto, Koichiro Ushijima

    2020.3 

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    Event date： 2020.3.21 - 2020.3.22

    Language：Japanese   Presentation type：Poster presentation  

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  • Non-destructive detection of water-soaked flesh fruit using an acoustic vibration method in ‘Hakuho’ peach

    Fumio Fukuda, Ryohei Iwai, Itsuki Takashima, Takashi Kawai, Ken Hirano, Naoki Sakurai

    2020.3 

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    Event date： 2020.3.21 - 2020.3.22

    Language：Japanese   Presentation type：Poster presentation  

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  • Effects of rootstock varieties and ABA-treatment on fruit aromatic components in ‘Shine-muscat’ grapes

    Hideo Yuki, Ryohei Iwai, Yoko Nakano, Takashi Kawai, Ken Hirano, Fumio Fukuda

    2020.3 

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    Event date： 2020.3.21 - 2020.3.22

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Demonstration experiment of export to Singapore in 'Nakatani-Wase' persimmon

    Takahiro Furuta, Manabu Wanaka, Hirokazu Ikenaga, Takashi Kawai, Yosuke Fukamatsu, Fumio Fukuda, Ryohei Nakano

    2019.9 

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    Event date： 2019.9.15 - 2019.9.17

    Language：Japanese   Presentation type：Poster presentation  

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  • 六倍体カキの樹内雌雄バランスに関する倍数体用GWAS解析

    Kanae Masuda, Kenta Shirasawa, Eiji Yamamoto, Takashi Kawai, Noriyuki Onoue, Jun Kono, Koichiro Ushijima, Yasutaka Kubo, Ryutaro Tao, Takashi Akagi

    2019.9 

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    Event date： 2019.9.6 - 2019.9.7

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Nondestructive detection of inner disorders in peach fruit using an acoustic vibration method

    Takashi Kawai

    Meru University of Science and Technology-JICA/NACOSTI Biotechnology Research Workshop  2019.8 

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    Event date： 2019.8.30

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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  • Nondestructive detection of inner disorders in peach fruit using an acoustic vibration method

    Takashi Kawai

    NACOSTI/JSPS Bilateral Research Special Joint Seminar  2019.8 

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    Event date： 2019.8.28

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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  • 海運による果物の輸出において透湿度を制御した段ボール箱が箱の強度保持及び果物蒸散抑制に及ぼす影響~3~

    田中章雄, 藤本丹民, 志水基修, 牧内隆文, 所 のぞみ, 河端浩平, 中野龍平, 福田文夫, 河井 崇, 深松陽介, 安永円理子, 高田大輔, 中村宣貴, 兼田朋子

    第28回日本包装学会年次大会. 研究発表会予稿集28: 128-129  2019.7 

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    Event date： 2019.7.11 - 2019.7.12

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Nondestructive detection of split-pit and estimation of maturity in on-tree peach fruit using mobile prototype machines of an acoustic vibration method

    Fumio Fukuda, Takashi Kawai, Fumiya Matsumori, Yosuke Fukamatsu, Hidemi Akimoto, Naoki Sakurai, Ryohei Nakano

    2019.3 

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    Event date： 2019.3.23 - 2019.3.24

    Language：Japanese   Presentation type：Poster presentation  

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  • Characterization of autocatalytic ethylene production and fruit softening in ‘Tosui’ peach

    Takashi Kawai, Kagari Akita, Sakine Watanabe, Yuna Hashimoto, Yosuke Fukamatsu, Daisuke Takata, Fumio Fukuda, Ryohei Nakano

    2019.3 

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    Event date： 2019.3.23 - 2019.3.24

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Nondestructive detection of split pit in peach fruit with an acoustic vibration method

    Takashi Kawai

    Jilin University-Okayama University Joint Sakura Science Workshop  2018.12 

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    Event date： 2018.12.17 - 2018.12.18

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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  • カキの雌雄花比に関するゲノムワイドアソシエーション解析

    Kanae Masuda, Takashi Akagi, Kenta Shirasawa, Takashi Kawai, Noriyuki Onoue, Jun Kono, Ryutaro Tao

    2018.9 

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    Event date： 2018.9.22 - 2018.9.24

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 海運による果物の輸出において透湿度を制御した段ボール箱が箱の強度保持及び果物蒸散抑制に及ぼす影響~2~

    田中章雄, 小野嘉則, 志水基修, 牧内隆文, 所 のぞみ, 渡辺朱音, 中野龍平, 福田文夫, 河井 崇, 有松仁美, 安永円理子, 高田大輔, 中村宣貴, 兼田朋子

    第27回日本包装学会年次大会. 研究発表会予稿集27: 100-101  2018.7 

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    Event date： 2018.7.12 - 2018.7.13

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Long-term storage of 'Aurora black' grape using high performance container and MA packaging and their export

    Yuna Hashimoto, Shiori Nakada, Ryohei Iwai, Takashi Kawai, Fumio Fukuda, Koichi Shutou, Kenji Ogura, Hirokazu Ikenaga, Reiko Nishime, Ryohei Nakano

    2018.3 

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    Event date： 2018.3.24 - 2018.3.25

    Language：Japanese   Presentation type：Poster presentation  

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  • Nondestructive detection of split-pit peach fruit on tree with an acoustic vibration method

    Takashi Kawai, Fumiya Matsumori, Hidemi Akimoto, Naoki Sakurai, Ryohei Nakano, Fumio Fukuda

    2018.3 

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    Event date： 2018.3.24 - 2018.3.25

    Language：Japanese   Presentation type：Poster presentation  

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  • 音響振動法を用いた樹上でのモモ核割れ果の非破壊判別

    Takashi Kawai

    2017.12 

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    Event date： 2017.12.22

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • 夏期集中講義における食育：スマートフォンを活用した調理実習指導

    Machiko Yamashita, Tamaki Hosotsuji, Hiroki Saito, Rihito Takisawa, Takashi Kawai, Tetsuya Nakazaki, Akira Kitajima

    2017.8 

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    Event date： 2017.8.31 - 2017.9.1

    Language：Japanese   Presentation type：Poster presentation  

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  • モモ果実内部障害の非破壊判別技術

    Takashi Kawai

    2017.6 

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    Event date： 2017.6.30

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Identification of QTLs controlling the temperature requirements for dormancy release and bud break in Japanese apricot (Prunus mume)

    Yuto Kitamura, Hisayo Yamane, Tsuyoshi Habu, Kei Kajita, Takuya Sobue, Takashi Kawai, Tetsuya Nakazaki, Akira Kitajima, Ryutaro Tao

    2017.3 

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    Event date： 2017.3.19 - 2017.3.20

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • ブドウ果房周辺の遮光処理が日射量，果房内温度および果粒品質に及ぼす影響

    Takashi Kurosawa, Takashi Kawai, Tsuyoshi Konishi, Katsutoshi Nonaka, Koji Kusumi, Yukari Yasui, Akira Kitajima

    2017.3 

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    Event date： 2017.3.8 - 2017.3.10

    Language：Japanese   Presentation type：Poster presentation  

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  • カキ‘太秋’のマルチ灌水栽培システムにおける果実収量と品質に及ぼす着蕾数の影響

    Yukari Yasui, Tsuyoshi Konishi, Katsutoshi Nonaka, Koji Kusumi, Takashi Kurosawa, Takashi Kawai, Akira Kitajima

    2017.3 

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    Event date： 2017.3.8 - 2017.3.10

    Language：Japanese   Presentation type：Poster presentation  

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  • Sex determination in oriental persimmon (Diospyros kaki)

    Takashi Akagi, Isabelle M. Henry, Takashi Kawai, Luca Comai, Ryutaro Tao

    Plant & Animal Genome Conference XXV  2017.1 

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    Event date： 2017.1.14 - 2017.1.18

    Language：English   Presentation type：Oral presentation (general)  

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  • Epigenetic memory controls the flexible sexuality in persimmon (I)

    Takashi Akagi, Isabelle M. Henry, Takashi Kawai, Luca Comai, Ryutaro Tao

    2016.3 

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    Event date： 2016.3.26 - 2016.3.27

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Construction of a Japanese apricot (Prunus mume) linkage map for identifying chilling requirement QTL

    Hisayo Yamane, Tsuyoshi Habu, Takuya Sobue, Takashi Kawai, Tetsuya Nakazaki, Akira Kitajima, Ryutaro Tao

    2016.3 

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    Event date： 2016.3.26 - 2016.3.27

    Language：Japanese   Presentation type：Poster presentation  

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  • Role of the sex determinant of Diospyros in complex sexuality in persimmon cultivars

    Takashi Kawai, Takashi Akagi, Ryutaro Tao

    2015.3 

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    Event date： 2015.3.28 - 2015.3.29

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Investigation of phylogeny and species differentiation in Citrus by chloroplast DNA Analysis

    A. Kitajima, M. Yamamoto, A. Yamasaki, M. Nakano, T. Shimizu, S Koeda, T. Habu, T. Fudano, S. Inafuku, J. Kaneyoshi, M. Fumuro, K. Katsura, H. Saito, R. Takisawa, T. Kawai, Y. Abe, X. Li, L. Yue, X. Yang, G. Zhong, X. Yang, D. Zhuang, T. Hsiung, H. Yen, H. T. Thi, C. Yapwattanaphun, N. Nasir, K. Yonemori, T. Nakazaki

    3rd International Symposium on Citrus Biotechnology  2014.11 

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    Event date： 2014.11.11 - 2014.11.14

    Language：English   Presentation type：Oral presentation (general)  

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  • Study on fruit abscission mechanism during physiological fruit drop in Citrus

    X. Li, A. Kitajima, K. Katsura, H. Saito, S. Koeda, R. Takisawa, T. Kawai, T. Nakazaki, T. Shimizu

    3rd International Symposium on Citrus Biotechnology  2014.11 

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    Event date： 2014.11.11 - 2014.11.14

    Language：English   Presentation type：Oral presentation (general)  

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  • ウメF1分離集団における休眠性の遺伝様式の調査

    Takuya Sobue, Takashi Kawai, Hisayo Yamane, Tetsuya Nakazaki, Akira Kitajima

    2014.9 

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    Event date： 2014.9.27 - 2014.9.29

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 四倍体ブドウの無核短梢栽培における果粒品質改善の試み

    Takashi Kurosawa, Takashi Kawai, Daiki Matsumoto, Tsuyoshi Konishi, Katsutoshi Nonaka, Koji Kusumi, Masaru Matsuda, Akira Kitajima

    2014.9 

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    Event date： 2014.9.4 - 2014.9.5

    Language：Japanese   Presentation type：Poster presentation  

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  • これからの育種に向けた新技術

    Takashi Kawai

    2014.8 

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    Event date： 2014.8.10

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Development of ALSV-mediated VIGS in Prunus fruit trees

    Takashi Kawai, Michiya Nitta, Ayako Gonoi, Ryutaro Tao

    7th International Rosaceae Genomics Conference  2014.6 

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    Event date： 2014.6.24 - 2014.6.26

    Language：English   Presentation type：Poster presentation  

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  • ALSV-mediated VIGS in fruit tree species in Prunus

    Ayako Gonoi, Takashi Kawai, Ryutaro Tao

    7th International Cherry Symposium  2013.6 

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    Event date： 2013.6.23 - 2013.6.27

    Language：English   Presentation type：Oral presentation (general)  

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  • Development of the ALSV-mediated gene evaluation system for apricot

    Takashi Kawai, Ayako Gonoi, Noriko Yamagishi, Nobuyuki Yoshikawa, Masanori Kaido, Ryutaro Tao

    2013.3 

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    Event date： 2013.3.23 - 2013.3.24

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • ALSV-mediated VIGS in apricot (Prunus armeniaca)

    Takashi Kawai, Ryutaro Tao

    Plant & Animal Genome Conference XXI  2013.1 

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    Event date： 2013.1.12 - 2013.1.16

    Language：English   Presentation type：Poster presentation  

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  • ベビーパーシモン生産に向けたカキ ‘突核無’ 果実のmRNA-seq解析

    Tsuyoshi Habu, Takashi Kawai, Hisayo Yamane, Ryutaro Tao

    2012.9 

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    Event date： 2012.9.22 - 2012.9.24

    Language：Japanese   Presentation type：Poster presentation  

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  • Development of the ALSV-mediated gene evaluation system for Prunus

    Takashi Kawai, Erika Nagayama, Takashi Akagi, Hisayo Yamane, Noriko Yamagishi, Nobuyuki Yoshikawa, Ryutaro Tao

    2012.3 

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    Event date： 2012.3.28 - 2012.3.29

    Language：Japanese   Presentation type：Poster presentation  

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  • Development of the ALSV-mediated gene evaluation system for Prunus

    Takashi Kawai, Erika Nagayama, Takashi Akagi, Hisayo Yamane, Ryutaro Tao

    Plant & Animal Genome Conference XX  2012.1 

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    Event date： 2012.1.14 - 2012.1.18

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  • Development of a gene evaluation system with virus-induced gene silencing in Prunus

    Takashi Akagi, Erika Nagayama, Takashi Kawai, Hisayo Yamane, Ryutaro Tao

    5th International Rosaceae Genomics Conference  2010.11 

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    Event date： 2010.11.14 - 2010.11.17

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Awards

  • Outstanding Presentation Award (R6 spring annual meeting)

    2024.3   Japanese Society for Horticultural Science   Presumption of fruit maturity and prediction of harvest time using acoustic vibration method in late ripening 'Hakuo' peach

    Ryusei Mukai, Kakeru Hatakeyama, Go Inohara, Takashi Kawai, Ken Hirano, Fumio Fukuda

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  • Outstanding Paper Award (H30)

    2019.3   Japanese Society for Horticultural Science   Nondestructive detection of split pit in peaches using an acoustic vibration method

    Ryohei Nakano, Hidemi Akimoto, Fumio Fukuda, Takashi Kawai, Koichiro Ushijima, Yosuke Fukamatsu, Yasutaka Kubo, Yuichiro Fujii, Ken Hirano, Kunihisa Morinaga, Naoki Sakurai

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  • Outstanding Paper Award (H26)

    2015.3   Japanese Society for Horticultural Science   Development of molecular markers associated with sexuality in Diospyros lotus L. and their application in D. kaki Thunb.

    Takashi Akagi, Kei Kajita, Takanori Kibe, Haruka Morimura, Tomoyuki Tsujimoto, Soichiro Nishiyama, Takashi Kawai, Hisayo Yamane, Ryutaro Tao

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Research Projects

  • Transcriptome/Cistrome analysis of ethylene synthesis mutants to elucidate the regulation of fruit ripening

    Grant number：24K01747  2024.04 - 2029.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Koichiro Ushijima, Tomoaki Kashiwamoto, Takashi Akagi, Ryohei Nakano, Kazuki Ikeda, Takashi Kawai

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    Authorship：Coinvestigator(s) 

    Grant amount：\18590000 （ Direct expense: \14300000 、 Indirect expense：\4290000 ）

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  • 測量技術を活用したモモの着果位置と果実品質の関係の3Dモデル化

    2023.04 - 2024.03

    公益財団法人園芸振興松島財団  第49回（2022年度）研究助成 

    Takashi Kawai, Daisuke Takata, Fumio Fukuda

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    Authorship：Principal investigator 

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  • 「ダイバーシティ農業による地域イノベーション共創拠点」構想

    2022.10 - 2024.03

    日本科学技術振興機構（JST）  共創の場形成支援プログラム（COI-NEXT）地域共創分野・育成型 

    Yasuhiko Hayashi (PL)

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    Authorship：Coinvestigator(s) 

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  • 三次元点群データを用いた果樹類の樹体・果実情報の取得

    2022.10 - 2023.03

    岡山大学AI・数理データサイエンスセンターサイバーフィジカル情報応用研究推進部門 （Cypher）  令和4年度Society5.0研究支援プログラム 

    Takashi Kawai

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  • 肉質・成熟期が異なるモモの樹上における果実成熟様相の比較とその制御機構の解明

    Grant number：22K05614  2022.04 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Takashi Kawai, Fumio Fukuda

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    Authorship：Principal investigator 

    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

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  • New Frontier of Fruit Postharvest Physiology-Low Temperature modulated Ripening and its application

    Grant number：20H02977  2020.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Yasutaka Kubo, Kentaro Yano, Daigo Abe, Masaya Kato, Koichiro Ushijima, Takashi Akagi, Takashi Kawai

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    Authorship：Coinvestigator(s) 

    Grant amount：\17810000 （ Direct expense: \13700000 、 Indirect expense：\4110000 ）

    本研究は、低温誘導成熟機構を、温帯性果実種横断的にゲノム・トランスクリプトーム解析、メタボローム解析、遺伝子組換え技術などを駆使して解析し、その統合的理解を目指す先駆的学術的取り組みである。低温誘導成熟果はエチレン誘導果よりも品質保持期間が長く、低温感受性の品種間差異は成熟の早生・晩生性に密接に関与している。本研究は、収穫後ロス低減に直結する新品質保持技術開発と分子マーカー開発による育種への貢献を目的とする。
    本年度は、研究の第２段階として、ウンシュウミカン、カキ、セイヨウナシ、リンゴ果実でのRNAseq解析、LC解析に基づいて成熟制御システムの全体像を把握するとともに、低温応答遺伝子および代謝物を抽出を試みた。今回解析したいずれの果実でもエチレン処理および低温遭遇によって、細胞壁分解系遺伝子、クロロフィル分解系遺伝子発現が刺激されることが明らかになった。さらに、ウンシュウミカンとレモン果実の低温応答に関するRNAseqデータの解析によって、カンキツでは、共通してERF3遺伝子が低温応答に重要な転写因子候補であることが示された。また、 ‘小原紅早生’ミカンではエチレンシグナルと低温シグナルによりCCD4遺伝子が活性化され、アポカロテノイド類が特異的に蓄積して成熟時に紅色に着色することが示された。また、‘富有’カキでは５℃貯蔵によってエチレン生成が刺激され果肉軟化と水浸状化を特徴とする低温障害症状がが進行するが、20℃以上または０℃では果肉軟化が抑制された。ただし、０℃貯蔵では常温に移行すると急激にエチレン生成の誘導と果肉軟化が進行した。１ヶ月程度なら20℃の常温貯蔵が可能であることが示された

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  • 成熟期の多様性に焦点を当てた梅の品種育成

    2019.04 - 2024.03

    和歌山県果樹試験場うめ研究所  共同研究 

    Takashi Kawai, Koichiro Ushijima, Fumio Fukuda

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  • Study of on-tree nondestructive judgement of internal disorders and maturation of peach fruit by an acoustic vibration method

    Grant number：19K06018  2019.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Fumio Fukuda, Takashi Kawai

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    Authorship：Coinvestigator(s) 

    Grant amount：\4420000 （ Direct expense: \3400000 、 Indirect expense：\1020000 ）

    モモにおいて音響測定による非破壊判別を簡便に行えるようにするため、モモの核割れ果の判別する試験において、2点型の分析機器「聴診器」と一点型音響装置との間で、共鳴周波数などの音響データの整合性の把握を行った。果実発育中の継続的な変化を確認したところ、おおむね同様の結果となり、1点型試験機でも核割れ発生を判別でき、また果実袋上など直接果実にセンサーを接触させられない栽培状態でも共鳴周波数の取得が可能であることが明らかになった。導入したモバイル型音響装置についても調整を行い、核割れや樹上での成熟状態を把握できるように、プログラムを開発メーカーと調整し、2020年度に果実生産への利用可能性の検討ができるようにした。
    また、一点型音響測定装置を用いて、モモ’白鳳’において、70果実の果実発育中に樹上で共鳴周波数の低下を調査していき、水浸状果肉障害の発生を非破壊把握する方法を検討した。果肉断面の半分程度に水浸症状を呈する水浸指数2以上の商品価値が低下する果実については、正常果と比べて、第2共鳴周波数が著しく低下することから、この閾値を550Hzに設定することで、収穫果において商品性の低い果実の混入を抑制できることが明らかになった。さらに、樹上調査において、7月初めの段階で、ほぼ同じ着果位置でも、共鳴周波数の低い果実が存在し、そのような果実の多くが収穫時に水浸症状をとなっていることが確認された。この方法を利用することで、水浸果となっていく変化が生じ始める時期や着生させている枝の状況などとの関連を検討できることが明らかとなった。これらをまとめて、令和2年度園芸学会春季大会で発表した。

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  • Studies on regulatory mechanisms of ultra-late maturing trait in ‘Gento’ peach

    Grant number：19K06033  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Takashi Kawai, Fumio Fukuda, Ryohei Nakano, Koichiro Ushijima

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    Authorship：Principal investigator 

    Grant amount：\4420000 （ Direct expense: \3400000 、 Indirect expense：\1020000 ）

    本研究では，岡山県内の民間育成品種‘玄桃’において見出された極晩生の果実成熟特性に着目し，交雑後代を用いた遺伝学的解析，糖組成・植物ホルモン分析などの生理学的解析，次世代シークエンサーを用いた網羅的解析を組み合わせることで，果実の成熟時期に関わる新規遺伝子の同定を目指す．本年度は‘玄桃’後代を用いて果実成熟に関わる諸形質の評価を行い，本研究の基礎となる形質データおよびその遺伝様式・年次変動を確認した．また，モモの熟期への関与が示唆されているNAC遺伝子の遺伝子型と熟期との関連を調査した．
    ‘清水白桃’ב玄桃’F1後代，‘玄桃’自殖後代および親品種を供試し，満開日から収穫日までの日数を成熟日数として算出した．F1後代および自殖後代の果実肥大様相と成熟日数が親品種の形質の間で分離し，親品種と同程度の成熟日数を示す個体は見られなかった．F1後代は自殖後代より熟期が早く，また，F1後代内でも成熟日数が短い個体群と長い個体群に1:1分離した．前年度までに予備的に形質評価していた個体において，F1後代内における成熟日数の分離は今年度の結果と一致していた．第4連鎖群のNAC遺伝子について，親品種間で確認された多型の遺伝子型を後代の各個体で調査したところ，既報の9bpのindelがF1後代内における熟期の早晩と共分離した．以上より，‘冬桃がたり’の成熟遅延に関わる因子は顕性ではなくF1では分離しないこと，‘清水白桃’は熟期の制御に関わる効果の大きい主要因子をヘテロでもつことが示唆された．また，NAC遺伝子の9bpのindelが‘清水白桃’がヘテロでもつ主要因子，あるいはその因子と連鎖する多型である可能性が考えられた．

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  • モモの低温誘導性成熟機構の解明およびその園芸的利用

    2019.04 - 2020.03

    公益財団法人山陽放送学術文化財団  第56回（平成30年度）研究助成 

    Takashi Kawai

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    Authorship：Principal investigator 

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  • 極晩生モモ‘玄桃’の果実成熟特性およびその制御機構の解明

    2018.10 - 2019.09

    公益財団法人両備檉園記念財団  第40回（平成30年度）研究助成 

    Takashi Kawai, Fumio Fukuda

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  • Characterization of long shelf-life and pulp disorder-alleviating mechanisms using late-harvest rare peach cultivars bred private growers as genetic resource

    Grant number：18H02200  2018.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Ryohei Nakano, Koichiro Ushijima, Fumio Fukuda, Daisuke Takata, Takashi Kawai

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    Authorship：Coinvestigator(s) 

    Grant amount：\17030000 （ Direct expense: \13100000 、 Indirect expense：\3930000 ）

    本年度は、研究グループが注目している棚持ち性の優れる品種、障害発生の異なる枝変わり系統に関して、以下のような成果が得られた。
    １）収穫時期や栽培地域を問わず、‘桃水’では自己触媒的エチレン生合成能が欠損していること、外生エチレンに応答して果実が軟化することが明らかとなり、‘桃水’は硬肉モモに属することが示された。Tatsukiら(2018)による、エチレン生成能の欠損とIAAの生合成関連遺伝子へのトランスポゾンの挿入変異との関連についての報告を参考に、親である‘川中島白桃’および‘桃水’の挿入変異を解析したところ、‘川中島白桃’はヘテロで‘桃水’はホモにて変異を持つことが明らかとなった。さらに、関連する数品種の全ゲノム配列を予備的に調査したところ挿入変異をヘテロで持つ‘ゆうそら’が片親である可能性が示唆された。‘桃水’は外生エチレン処理時の肉質が優れており、韓国系の品種を用いず、日本にて育成された品種同士の交配による高品質かつ棚持ち性の良い品種育成が実証された。
    ２）‘大寿蜜桃’ は収穫時期、栽培地域によらず、自己触媒的エチレン生成能を持つにも関わらず、エチレンによって軟化せず、3週間以上の棚持ち性をした。一方、‘冬美白’ではエチレン生成とともに、果肉軟化が進行し、通常のモモと同様のエチレン応答性を示した。
    ３）‘紅博桃’およびその亜主枝単位の枝変わりについて調査したところ、枝変わりでは生長第２期（肥大停止期）から３期（最終肥大期）への転換が遅れており、熟期が3週間ほど遅れことや収穫後に蜜症障害が多発することが明らかとなった。
    ４）上記の１）－３）の品種や系統に関して、予備的な全ゲノムシークエンス解析を実施し、‘桃水’と‘川中島白桃’の親子関係や、‘紅博桃’樹内の熟期が異なる枝の枝変わり関係を確認するとともに、詳細な解析に向けた方向性を確認した。

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  • Development of a gene evaluation system using virus vectors in Prunus and its potential application to Prunus breeding

    Grant number：16K18651  2016.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Takashi Kawai

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    Authorship：Principal investigator 

    Grant amount：\4030000 （ Direct expense: \3100000 、 Indirect expense：\930000 ）

    In this study, we attempted to use Apple latent spherical virus (ALSV) vectors for functional analysis of genes associated with floral induction and thus to induce precocious flowering in Prunus fruit tree species. ALSV vectors carrying the full-length sequence of FT gene or a partial sequence of TFL1 gene could be successfully inoculated into several cultivars of apricot, sweet cherry, and almond by particle bombardment, although early floral induction could not be observed in infected plants. Further optimization of viral inoculation procedures and growth conditions for infected plants will lead to the full use of ALSV vectors for evaluation of gene functions or various practical studies in Prunus.

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  • Functional characterization of DAMs involved in seasonal growth and dormancy regulation in fruit tree species of Rosaceae

    Grant number：26252005  2014.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    Hisayo Yamane, Ryutaro Tao, Tsuyoshi Habu, Mei Takai, Kiyoshi Banno, Takashi Kawai, Yuto Kitamura, Takuya Sobue, Ayaka Sugimoto, Shotaro Mimura, Hideyuki Iwasa, Takanori Takeuchi, Kei Kajita, Soichiro Nishiyama, Kanae Masuda, Miwako Matsushita, Chikako Honda, Akira Oikawa, Mineyuki Yokoyama, Ryuta Sasaki, Kentaro Yano, Hiroshi Fujii, Tokuro Shimizu, Toshiya Yamamoto, Kazuo Ikeda, Takuya Tetsumura, Tetsuya Nakazaki, Akira Kitajima, Kazuma Okada

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    Authorship：Coinvestigator(s) 

    Grant amount：\40690000 （ Direct expense: \31300000 、 Indirect expense：\9390000 ）

    The purpose of this study is to characterize the function of DAM, candidate master regulator of dormancy regulation in Prunus, by transformation studies, QTL analysis, and expression analysis using various fruit tree species belonging to Rosaceae. We demonstrated that Prunus DAM6 controls chilling requirement for dormancy release and delayed bud break using transgenic studies. In addition, eQTL of Prunus DAM6 colocalized with QTLs controlling chilling requirement, heat requirement and bud break date of Prunus mume, suggesting that Prunus DAM6 acts as a main causal factor controlling dormancy. On the other hand, DAMs in apple appeared not to have crucial roles in the regulation of chilling requirement. Alternatively, we identified putative candidates of master regulators for dormancy release in apple, such as FLC. Collectively, our study provided important knowledge about the role of DAM and molecular regulation of dormancy in fruit tree species in Rosaceae.

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  • Elucidation of the molecular basis of sex determination in Diospyros species by means of NGS technologies

    Grant number：26660025  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    Ryutaro Tao, Takashi Kawai

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    Authorship：Coinvestigator(s) 

    Grant amount：\4030000 （ Direct expense: \3100000 、 Indirect expense：\930000 ）

    A combination of NGS technologies and evolutionary approaches detected a Y-specific sex-determinant candidate small RNA gene, OGI targeting the autosomal MeGI gene in Diospyros lotus. Then, the molecular basis underlying sex determination in polyploid D. kaki was investigated. OGI appeared to be indispensable for the monoecious phenotype producing male flowers in D. kaki, as in dioecious diploid D. lotus. However, the existence of OGI alleles did not always lead to production of male flowers. A series of NGS analyses indicated that there is an additional layer of regulation in the form of DNA methylation of the MeGI promoter, which seemed to be associated with the production of both male and female flowers in genetically male trees in D. kaki. Concurrently, in Y-chromosome-carrying trees, the expression of OGI is silenced by the presence of a SINE-like insertion in the OGI promoter.

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  • Development of a gene evaluation system using virus vectors in Prunus

    Grant number：26850016  2014.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Takashi Kawai

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    Authorship：Principal investigator 

    Grant amount：\3900000 （ Direct expense: \3000000 、 Indirect expense：\900000 ）

    Virus-induced gene silencing (VIGS) has been used as a rapid and effective tool for functional analysis of genes in various plants. In this study, we assessed the VIGS efficiency of Apple latent spherical virus (ALSV) vectors in a wide range of Prunus species and cultivars. We found that ALSV vectors could successfully induce VIGS of endogenous PHYTOENE DESATURASE (PDS) genes in several cultivars of apricot, sweet cherry, and almond, although ALSV infectivity and VIGS efficiency seemed to vary depending on species and/or cultivar in Prunus. Further optimization of viral inoculation procedures and growth conditions for infected plants will lead to the full use of ALSV vectors for evaluation of gene functions or various practical studies in Prunus.

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  • Development of a gene evaluation system using virus vectors in Prunus

    2013.10 - 2014.03

    Kyoto University  平成25年度第Ⅱ期京都大学若手研究者スタートアップ研究費 

    Takashi Kawai

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  • Fruit Science（果実の科学） （2024academic year） 第３学期  - 金5～6

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  • Laboratory in Applied Plant Science 1 （2022academic year） 1st and 2nd semester  - 木5～8

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  • コース演習4 （2022academic year） ３・４学期  - 水7,水8,水9

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  • Experiment in Applied Plant Science 1 （2022academic year） 1st and 2nd semester  - 木5,木6,木7,木8

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  • Laboratory in Applied Plant Science 1 （2021academic year） 1st and 2nd semester  - 金5,金6,金7,金8

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  • Laboratory in Applied Plant Science 1 （2020academic year） 1st and 2nd semester  - 木5,木6,木7,木8

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  • Experiment in Applied Plant Science 1 （2020academic year） 1st and 2nd semester  - 木5,木6,木7,木8

  • Experiment in Applied Plant Science 2 （2020academic year） 1st and 2nd semester  - 金5,金6,金7,金8

  • Experiment in Applied Plant Science 3 （2020academic year） 3rd and 4th semester  - 木5,木6,木7,木8

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Social Activities

  • 2024岡山大学農学部オープンキャンパス 植物コースツアーガイド

    Role(s)：Planner

    2024.8.8

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  • 令和5年度岡山県高等学校農業教育協会園芸班研究協議会 実地研修指導

    Role(s)：Lecturer

    2023.12.6

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  • 高大連携事業・講師派遣（岡山県立高梁高等学校） 学部紹介＋研究関連講義「モモとブドウの果実に関する基礎・応用研究」

    Role(s)：Lecturer

    2023.10.6

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  • 岡山県立高松農業高等学校校外学習 研究紹介

    Role(s)：Lecturer

    2022.10.27

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  • 2022岡山大学農学部オープンキャンパス 植物コースツアーガイド

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    2022.8.10

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Media Coverage

  • 岡山大学農学部研究成果プレスリリース モモの核割れ発生の樹上判別法の開発について山陽新聞に掲載

    2018.6

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