2025/01/09 更新

写真a

マツバラ タケヒロ
松原 岳大
Matubara Takehiro
所属
総合技術部 技術専門職員
職名
技術専門職員
外部リンク

学位

  • 博士(医学) ( 神戸大学 )

  • 修士(生物) ( 神戸大学 )

  • 学士(生物) ( 神戸大学 )

研究キーワード

  • バイオバンク

  • シグナル伝達

  • signal transduction

研究分野

  • ライフサイエンス / 医化学

学歴

  • 神戸大学   Graduate School, Division of Medical Sciences  

    - 2008年

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  • 神戸大学    

    - 2008年

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    国名: 日本国

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  • 神戸大学   Graduate School, Division of Science and Technology  

    - 2005年

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  • 神戸大学    

    - 2005年

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    国名: 日本国

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  • 神戸大学   Faculty of Science  

    - 2003年

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  • 神戸大学   Faculty of Science   Department of Biology

    - 2003年

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    国名: 日本国

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▼全件表示

経歴

  • 岡山大学   バイオバンク   技術専門職員

    2014年2月 - 現在

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    国名:日本国

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  • - 長崎大学大学院医歯薬学総合研究科新興感染症病態制御学系専攻新興感染症病態制御学系専攻(医) 助教

    2008年

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  • - Assistant Professor,Infection Research,Infection Research,Graduate School of Biomedical Sciences,Nagasaki University

    2008年

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所属学協会

 

MISC

  • Prion protein interacts with the metabotropic glutamate receptor 1 and regulates the organization of Ca2+ signaling. 国際誌

    Takehiro Matsubara, Katsuya Satoh, Takujiro Homma, Takehiro Nakagaki, Naohiro Yamaguchi, Ryuichiro Atarashi, Yuka Sudo, Yasuhito Uezono, Daisuke Ishibashi, Noriyuki Nishida

    Biochemical and biophysical research communications   525 ( 2 )   447 - 454   2020年4月

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    記述言語:英語  

    Cellular prion protein (PrP) is a membrane protein that is highly conserved among mammals and mainly expressed on the cell surface of neurons. Despite its reported interactions with various membrane proteins, no functional studies have so far been carried out on it, and its physiological functions remain unclear. Neuronal cell death has been observed in a PrP-knockout mouse model expressing Doppel protein, suggesting that PrP might be involved in Ca2+ signaling. In this study, we evaluated the binding of PrP to metabotropic glutamate receptor 1 (mGluR1) and found that wild-type PrP (PrP-wt) and mGluR1 co-immunoprecipitated in dual-transfected Neuro-2a (N2a) cells. Fluorescence resonance energy transfer analysis revealed an energy transfer between mGluR1-Cerulean and PrP-Venus. In order to determine whether PrP can modulate mGluR1 signaling, we performed Ca2+ imaging analyses following repetitive exposure to an mGluR1 agonist. Agonist stimulation induced synchronized Ca2+ oscillations in cells coexpressing PrP-wt and mGluR1. In contrast, N2a cells expressing PrP-ΔN failed to show ligand-dependent regulation of mGluR1-Ca2+ signaling, indicating that PrP can bind to mGluR1 and modulate its function to prevent irregular Ca2+ signaling and that its N-terminal region functions as a molecular switch during Ca2+ signaling.

    DOI: 10.1016/j.bbrc.2020.02.102

    PubMed

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  • Possible involvement of mGluR1 in the neurodegeration induced by prion-like protein, Doppel

    Takehiro Matsubara, Naohiro Yamaguchi, Yuka Sudo, Suehiro Sakaguchi, Yuko Ando, Noriyuki Nishida, Yasuhito Uezono

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   154P - 154P   2009年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

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  • mu-Opioid receptors form constitutive and agonist stimulation-independent heterodimer with cannabinoid CB1 receptors

    Yuka Sudo, Minoru Hojo, Yuko Ando, Masafumi Takada, Takehiro Matsubara, Masato Kanaide, Kohtaro Taniyama, Koji Sumikawa, Yasuhito Uezono

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   77P - 77P   2009年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:JAPANESE PHARMACOLOGICAL SOC  

    Web of Science

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  • mu-Opioid Receptor Forms a Functional Heterodimer With Cannabinoid CB(1) Receptor: Electrophysiological and FRET Assay Analysis

    Minoru Hojo, Yuka Sudo, Yuko Ando, Koichiro Minami, Masafumi Takada, Takehiro Matsubara, Masato Kanaide, Kohtaro Taniyama, Koji Sumikawa, Yasuhito Uezono

    JOURNAL OF PHARMACOLOGICAL SCIENCES   108 ( 3 )   308 - 319   2008年11月

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    記述言語:英語   出版者・発行元:JAPANESE PHARMACOLOGICAL SOC  

    Interactions between mu-opioid receptor (mu OR) and cannabinoid CB(1) receptor (CB(1)R) were examined by morphological and electrophysiological methods. In baby hamster kidney (BHK) cells coexpressing mu OR fused to the yellow fluorescent protein Venus and CB(1)R fused to the cyan fluorescent protein Cerulean, both colors were detected on the cell surface; and fluorescence resonance energy transfer (FRET) analysis revealed that mu OR and CB(1)R formed a heterodimer. Coimmunoprecipitation and Western blotting analyses also confirmed the heterodimers of mu OR and CB(1)R. [D-Ala(2),N-Me-Phe(4),Gly(5)-ol]enkephalin (DAMGO) or CP55,940 elicited K(+) currents in Xenopus oocytes expressing mu OR or CB(1)R together with G protein activated-inwardly rectifying K(+) channels (GIRKs), respectively. In oocytes coexpressing both receptors, either of which was fused to the chimeric G alpha protein G(qi5) that activates the phospholipase C pathway, both DAMGO and CP55,940 elicited Ca(2+)-activated Cl(-) currents, indicating that each agonist can induce responses through Gqi5 fused to either its own receptor or the other. Experiments with endogenous G(i/o). protein inactivation by pertussis toxin (PTX) supported the functional heterodimerization of mu OR/CB(1)R through PTX-insensitive G(qi5(m)) fused to each receptor. Thus, mu OR and CB(1)R form a heterodimer and transmit a signal through a common G protein. Our electrophysiological method could be useful for determination of signals mediated through heterodimerized G protein-coupled receptors.

    DOI: 10.1254/jphs.08244FP

    Web of Science

    CiNii Article

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講演・口頭発表等

  • Possible involvement of mGluR1 in the neurodegeration induced by prion-like protein, Doppel.

    日本薬理学会  2009年 

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    会議種別:ポスター発表  

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  • Cellular prion protein binds to metabotropic glutamate receptor type I and regulates its function

    日本分子生物学会  2009年 

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    会議種別:ポスター発表  

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共同研究・競争的資金等の研究

  • プリオン関連蛋白質による神経細胞変性死へのグルタミン酸受容体の関与とその分子機構

    2009年

    科学研究費補助金 

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    資金種別:競争的資金

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    資金種別:競争的資金

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