Updated on 2025/06/15

写真a

 
Matubara Takehiro
 
Organization
Department of Comprehensive Technical Solutions Technical Expert staff
Position
Technical Expert staff
External link

Degree

  • 学士(生物) ( 神戸大学 )

  • 博士(医学) ( 神戸大学 )

  • 修士(生物) ( 神戸大学 )

Research Interests

  • bioank

  • シグナル伝達

  • signal transduction

  • サイトカイン

  • がん

  • 次世代シークエンサー

Research Areas

  • Life Science / Medical biochemistry

Education

  • Kobe University   医学系研究科   医科学

    - 2008

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    Country: Japan

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  • Kobe University   自然科学研究科   生物学

    - 2005

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    Country: Japan

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  • Kobe University   理学部   生物学科

    - 2003

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    Country: Japan

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Research History

  • バイオリポジトリ技術管理士取得

    2021

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  • Okayama University   Biobank   Lab Technician

    2014.2

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    Country:Japan

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  • - 長崎大学大学院医歯薬学総合研究科新興感染症病態制御学系専攻新興感染症病態制御学系専攻(医) 助教

    2008

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  • - Assistant Professor,Infection Research,Infection Research,Graduate School of Biomedical Sciences,Nagasaki University

    2008

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Professional Memberships

 

Papers

  • DV200 Index for Assessing RNA Integrity in Next-Generation Sequencing. Reviewed International journal

    Takehiro Matsubara, Junichi Soh, Mizuki Morita, Takahiro Uwabo, Shuta Tomida, Toshiyoshi Fujiwara, Susumu Kanazawa, Shinichi Toyooka, Akira Hirasawa

    BioMed research international   2020   9349132 - 9349132   2020

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Poor quality of biological samples will result in an inaccurate analysis of next-generation sequencing (NGS). Therefore, methods to accurately evaluate sample integrity are needed. Among methods for evaluating RNA quality, the RNA integrity number equivalent (RINe) is widely used, whereas the DV200, which evaluates the percentage of fragments of >200 nucleotides, is also used as a quality assessment standard. In this study, we compared the RINe and DV200 RNA quality indexes to determine the most suitable RNA index for the NGS analysis. Seventy-one RNA samples were extracted from formalin-fixed paraffin-embedded tissue samples (n = 30), fresh-frozen samples (n = 25), or cell lines (n = 16). After assessing RNA quality using the RINe and DV200, we prepared two kinds of stranded mRNA sequencing libraries. Finally, we calculated the correlation between each RNA quality index and the amount of library product (1st PCR product per input RNA). The DV200 measure showed stronger correlation with the amount of library product than the RINe (R 2 = 0.8208 for the DV200 versus 0.6927 for the RINe). Receiver operating characteristic curve analyses revealed that the DV200 was the better marker for predicting efficient library production than the RINe using a threshold of >10 ng/ng for the amount of the 1st PCR product per input RNA (cutoff value for the RINe and DV200, 2.3 and 66.1%; area under the curve, 0.99 and 0.91; sensitivity, 82% and 92%; and specificity, 93% and 100%, respectively). Our results indicate that NGS libraries prepared using RNA samples with the DV200 value > 66.1% exhibit greater sensitivity and specificity than those prepared with the RINe values > 2.3. These findings suggest that the DV200 is superior to the RINe, especially for low-quality RNA, because it is a more consistent assessment of the amount of the 1st NGS library product per input.

    DOI: 10.1155/2020/9349132

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  • Effects of Cold Ischemia on RNA Stability and Quality of Lung Tissues Based on Standard PREanalytical Code Categorization. Reviewed International journal

    Takehiro Matsubara, Shuta Tomida, Junichi Soh, Takahiro Uwabo, Yoshiko Mori, Mizuki Morita, Yasutomo Nasu, Susumu Kanazawa, Shinichi Toyooka

    Biopreservation and biobanking   15 ( 5 )   484 - 486   2017.10

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    DOI: 10.1089/bio.2017.0076

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  • c-Abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase α by phosphorylation at Tyr-218. Reviewed International journal

    Takehiro Matsubara, Momo Ikeda, Yuko Kiso, Megumi Sakuma, Ken-ichi Yoshino, Fumio Sakane, Isabel Merida, Naoaki Saito, Yasuhito Shirai

    The Journal of biological chemistry   287 ( 8 )   5507 - 17   2012.2

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    c-Abl is a tyrosine kinase involved in many cellular processes, including cell cycle control and proliferation. However, little is known about its substrates. Here, we show that c-Abl directly phosphorylates diacylglycerol kinase α (DGKα), an important regulator of many cellular events through its conversion of diacylglycerol to phosphatidic acid. We found that DGKα was transported from the cytoplasm to the nucleus in response to serum starvation, and serum restoration induced the nuclear export of the enzyme to the cytoplasm. This serum-induced export involves two tyrosine kinases, c-Src and c-Abl. The latter, c-Abl, is activated by c-Src, phosphorylates DGKα, and shuttles between the nucleus and the cytoplasm in a direction opposite to that of DGKα in response to serum restoration. Moreover, an in vitro phosphorylation assay using purified mutants of DGKα identified Tyr-218 as a site of phosphorylation by c-Abl. We confirmed these results for endogenous DGKα using an antibody specific for phospho-Tyr-218, and this phosphorylation was necessary for the serum-induced export of DGKα. These results demonstrate that the nucleo-cytoplasmic shuttling of DGKα is orchestrated by tyrosine phosphorylation by the Src-activated tyrosine kinase c-Abl and that this phosphorylation is important for regulating the function of cytoplasmic and/or nuclear DGKα.

    DOI: 10.1074/jbc.M111.296897

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  • Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion Reviewed

    Ryuichiro Atarashi, Katsuya Satoh, Kazunori Sano, Takayuki Fuse, Naohiro Yamaguchi, Daisuke Ishibashi, Takehiro Matsubara, Takehiro Nakagaki, Hitoki Yamanaka, Susumu Shirabe, Masahito Yamada, Hidehiro Mizusawa, Tetsuyuki Kitamoto, Genevieve Klug, Amelia McGlade, Steven J. Collins, Noriyuki Nishida

    NATURE MEDICINE   17 ( 2 )   175 - 178   2011.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nm.2294

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  • Nuclear transportation of diacylglycerol kinase gamma and its possible function in the nucleus. Reviewed International journal

    Takehiro Matsubara, Yasuhito Shirai, Kei Miyasaka, Takuya Murakami, Yasuto Yamaguchi, Takehiko Ueyama, Masahiro Kai, Fumio Sakane, Hideo Kanoh, Toshiaki Hashimoto, Shinji Kamada, Ushio Kikkawa, Naoaki Saito

    The Journal of biological chemistry   281 ( 10 )   6152 - 64   2006.3

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Diacylglycerol kinases (DGKs) convert diacylglycerol (DG) to phosphatidic acid, and both lipids are known to play important roles in lipid signal transduction. Thereby, DGKs are considered to be a one of the key players in lipid signaling, but its physiological function remains to be solved. In an effort to investigate one of nine subtypes, we found that DGKgamma came to be localized in the nucleus with time in all cell lines tested while seen only in the cytoplasm at the early stage of culture, indicating that DGKgamma is transported from the cytoplasm to the nucleus. The nuclear transportation of DGKgamma didn't necessarily need DGK activity, but its C1 domain was indispensable, suggesting that the C1 domain of DGKgamma acts as a nuclear transport signal. Furthermore, to address the function of DGKgamma in the nucleus, we produced stable cell lines of wild-type DGKgamma and mutants, including kinase negative, and investigated their cell size, growth rate, and cell cycle. The cells expressing the kinase-negative mutant of DGKgamma were larger in size and showed slower growth rate, and the S phase of the cells was extended. These findings implicate that nuclear DGKgamma regulates cell cycle.

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MISC

  • Prion protein interacts with the metabotropic glutamate receptor 1 and regulates the organization of Ca2+ signaling. International journal

    Takehiro Matsubara, Katsuya Satoh, Takujiro Homma, Takehiro Nakagaki, Naohiro Yamaguchi, Ryuichiro Atarashi, Yuka Sudo, Yasuhito Uezono, Daisuke Ishibashi, Noriyuki Nishida

    Biochemical and biophysical research communications   525 ( 2 )   447 - 454   2020.4

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    Cellular prion protein (PrP) is a membrane protein that is highly conserved among mammals and mainly expressed on the cell surface of neurons. Despite its reported interactions with various membrane proteins, no functional studies have so far been carried out on it, and its physiological functions remain unclear. Neuronal cell death has been observed in a PrP-knockout mouse model expressing Doppel protein, suggesting that PrP might be involved in Ca2+ signaling. In this study, we evaluated the binding of PrP to metabotropic glutamate receptor 1 (mGluR1) and found that wild-type PrP (PrP-wt) and mGluR1 co-immunoprecipitated in dual-transfected Neuro-2a (N2a) cells. Fluorescence resonance energy transfer analysis revealed an energy transfer between mGluR1-Cerulean and PrP-Venus. In order to determine whether PrP can modulate mGluR1 signaling, we performed Ca2+ imaging analyses following repetitive exposure to an mGluR1 agonist. Agonist stimulation induced synchronized Ca2+ oscillations in cells coexpressing PrP-wt and mGluR1. In contrast, N2a cells expressing PrP-ΔN failed to show ligand-dependent regulation of mGluR1-Ca2+ signaling, indicating that PrP can bind to mGluR1 and modulate its function to prevent irregular Ca2+ signaling and that its N-terminal region functions as a molecular switch during Ca2+ signaling.

    DOI: 10.1016/j.bbrc.2020.02.102

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  • c-Abl phosphorylates DGK alpha at Y218 during the serum-induced nuclear export

    Momo Ikeda, Yasuhito Shirai, Takehiro Matsubara, Megumi Sakuma, Yuko Kiso, Naoaki Saito

    JOURNAL OF PHARMACOLOGICAL SCIENCES   115   167P - 167P   2011

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    Language:English   Publishing type:Research paper, summary (international conference)  

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  • 神経型ニコチン性アセチルコリン受容体(nAChRα7)活性化によって起こる受容体構造変化の解析

    須藤 結香, 堤 智崇, 銕尾 智幸, 安藤 優子, 松原 岳大, 谷山 紘太郎, 上園 保仁

    日本薬理学雑誌   133 ( 3 )   36P - 36P   2009.3

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    Language:Japanese   Publisher:(公社)日本薬理学会  

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  • Possible involvement of mGluR1 in the neurodegeration induced by prion-like protein, Doppel

    Takehiro Matsubara, Naohiro Yamaguchi, Yuka Sudo, Suehiro Sakaguchi, Yuko Ando, Noriyuki Nishida, Yasuhito Uezono

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   154P - 154P   2009

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    Language:English   Publishing type:Research paper, summary (international conference)  

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  • mu-Opioid receptors form constitutive and agonist stimulation-independent heterodimer with cannabinoid CB1 receptors

    Yuka Sudo, Minoru Hojo, Yuko Ando, Masafumi Takada, Takehiro Matsubara, Masato Kanaide, Kohtaro Taniyama, Koji Sumikawa, Yasuhito Uezono

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   77P - 77P   2009

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  • mu-Opioid Receptor Forms a Functional Heterodimer With Cannabinoid CB(1) Receptor: Electrophysiological and FRET Assay Analysis

    Minoru Hojo, Yuka Sudo, Yuko Ando, Koichiro Minami, Masafumi Takada, Takehiro Matsubara, Masato Kanaide, Kohtaro Taniyama, Koji Sumikawa, Yasuhito Uezono

    JOURNAL OF PHARMACOLOGICAL SCIENCES   108 ( 3 )   308 - 319   2008.11

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Presentations

  • Possible involvement of mGluR1 in the neurodegeration induced by prion-like protein, Doppel.

    日本薬理学会  2009 

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    Presentation type:Poster presentation  

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  • Cellular prion protein binds to metabotropic glutamate receptor type I and regulates its function

    日本分子生物学会  2009 

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    Presentation type:Poster presentation  

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Research Projects

  • プリオン関連蛋白質による神経細胞変性死へのグルタミン酸受容体の関与とその分子機構

    2009

    科学研究費補助金 

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    Grant type:Competitive

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  • -

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    Grant type:Competitive

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