研究者詳細 - 島内　寿徳

論文 - 島内　寿徳

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Membranomics Research on Interactions between Liposome Membranes with Membrane Chip Analysis

Shimanouchi Toshinori, Oyama Ena, Ishii Haruyuki, UMAKOSHI Hiroshi, KUBOI Ryoichi

膜 34 ( 6 ) 342 - 350 2009年11月

記述言語：英語出版者・発行元：THE MEMBRANE SOCIETY OF JAPAN

The liposome immobilized on indium tin–oxide (ITO) electrode was prepared to evaluate the liposome–liposome interaction to develop "Membrane Chip". Three kinds of standard liposomes entrapping the fluorescence probe, calcein, were used for their immobilization, where the immobilized liposomes show the different characteristics in their phase transition temperature and membrane fluidity. Membrane Chip could give the set of the quantitative information relating to the membrane–membrane interaction. The interaction between three immobilized liposomes and 22 kinds of liposomes as samples was then systematically investigated on a basis of the calcein release from the immobilized liposomes, which could give the information in relation to "Membranome". It was, therefore, found that the Membrane Chip as a means of Membranomics research was effective to evaluate the Membranome information of liposomes.

DOI： 10.5360/membrane.34.342

その他リンク： https://jlc.jst.go.jp/DN/JALC/00341564957?from=CiNii

Charged liposome affects the translation and folding steps of in vitro expression of green fluorescent protein

Hiroshi Umakoshi, Keishi Suga, Huong Thi Bui, Masato Nishida, Toshinori Shimanouchi, Ryoichi Kuboi

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 108 ( 5 ) 450 - 454 2009年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SOC BIOSCIENCE BIOENGINEERING JAPAN

The role of the charged liposome on the in vitro expression of green fluorescent protein (GFP) was investigated, focusing on its elemental steps such as transcription, translation and folding. The total GFP expression was enhanced to 145% when a neutral liposome (POPC: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocoline) was added externally to a cell-free translation system. On the contrary, the addition of the charged liposome composed of POPC with anionic 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (POPG) or cationic stearyl amine (SA) inhibited the total GFP expression, depending on the surface charge density of liposome. In transcription, the RNA synthesis was enhanced regardless of the variation of the surface charge, indicating that transcription was enhanced due to the stabilization of RNA structure by its hydrophobic interaction with liposome. Translation was inhibited by cationic liposome although it was enhanced by anionic liposome and neutral liposome. On the other hand, the folding was not inhibited in the presence of neutral liposome, whereas anionic liposome and cationic liposome inhibited the folding in proportion to the their surface charges, suggesting that the total GFP expression was controlled by a charged liposome in the translation step and folding step. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

DOI： 10.1016/j.jbiosc.2009.05.012

Analysis of interaction between liposome membranes induced by stress condition: Utilization of liposomes immobilized on indium tin oxide electrode 査読

Haruyuki Ishii, Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 108 ( 5 ) 425 - 428 2009年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SOC BIOSCIENCE BIOENGINEERING JAPAN

NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with alpha-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

DOI： 10.1016/j.jbiosc.2009.05.009

Preparation of liposome immobilized membrane module and its application 査読

Hiroshi Umakoshi, Hiroyuki Sugaya, Yuji Tohtake, Toshinori Shimanouchi, Ryoichi Kuboi

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 108 S65 - S65 2009年11月

Membrane interference: Role of liposome on in vitro gene expression of GFP 査読

Suga Keishi, Umakoshi Hiroshi, Bui Huong Thi, Tanabe Tomoyuki, Shimanouchi Toshinori, Kuboi Ryoichi

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 108 ( 1 ) S102 - S103 2009年11月

Development of membrane chip system for study on membrane-protein interaction 査読

Shimanouchi Toshinori, Ishii Haruyuki, Oyama Ena, Shimauchi Naoya, Umakoshi Hiroshi, Kuboi Ryoichi

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 108 S162 - S163 2009年11月

Disaggregation of catecholamine derivative-induced amyloid fibrils on biomembranes 査読

Shimanouchi Toshinori, Vu Huong Thi, Umakoshi Hiroshi, Kuboi Ryoichi

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 108 ( 1 ) S31 - S32 2009年11月

Calcein permeation across phosphatidylcholine bilayer membrane: Effects of membrane fluidity, liposome size, and immobilization

Toshinori Shimanouchi, Haruyuki Ishii, Noriko Yoshimoto, Hiroshi Umakoshi, Ryoichi Kuboi

COLLOIDS AND SURFACES B-BIOINTERFACES 73 ( 1 ) 156 - 160 2009年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

The permeation of calcein across the phospholipid bilayer membrane is a key phenomenon in the detection system using liposomes as a sensor unit. The behavior of the calcein release from the liposome was analyzed by a first-order kinetic to obtain the permeability coefficient, P(s) [cm/s]. The P(s) value for the neutral liposome, prepared by zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), was found to depend on both the diameter of liposome and the temperature. The membrane fluidity of the POPC liposome, evaluated by the hydrophobic probe, 1-(4-trimethyl-aminophenyl)-6-diphenyl-1,3,5-hexatriene, was also dependent on the liposome diameter and the temperature. The P, values for various neutral liposomes under gel phase or liquid-crystalline phase were correlated with their membrane fluidity, although some data were a little scattered, possibly due to the lamellarity. It is therefore considered that the membrane fluidity dominates the permeability of calcein across the neutral phospholipid membrane. Based on the above results, the P, value for liposomes immobilized on the solid surface is discussed. (C) 2009 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.colsurfb.2009.05.014

Characterization of heat-induced interaction of neutral liposome with lipid membrane of Streptomyces griseus cell

Kien Xuan Ngo, Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi

COLLOIDS AND SURFACES B-BIOINTERFACES 73 ( 2 ) 399 - 407 2009年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

The interaction between the neutral 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes and cell membrane of Streptomyces griseus induced by the heat treatment at specific temperature was investigated, focusing on the internalization of the neutral POPC liposomes with S. griseus cells. In an attempt to clarify the modes of liposome internalization, various kinds of inhibitors of endocytotic pathways were used to treat S. griseus cells. The efficiency of the heat treatment on liposome-cell membrane interactions was finally characterized based on the hydrophobic, electrostatic interactions and hydration effect. In fact, the internalization of the neutral liposomes into these cells was found to show higher rate and greater amount at higher temperatures. The kinetic study showed that the maximum amount of the internalized liposomes was, respectively, 469 x 10(5) and 643 x 10(5) liposomes/cell at 37 and 41 degrees C. The internalization of the neutral liposomes induced by the heat treatment was characterized, implying that the endocytosis occurred. The interactions involving the internalization, adsorption, and fusion of these liposomes with S. griseus cells were mainly contributed by the hydrophobic interaction and the unstable hydrogen bonds caused by the loss of water of surface hydration of cell membrane rather than the electrostatic interaction under the specific heat condition. (c) 2009 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.colsurfb.2009.06.015

Negatively charged liposome as a potent inhibitor of post-translation during in vitro synthesis of green fluorescent protein

Huong Thi Bui, Hiroshi Umakoshi, Keishi Suga, Masato Nishida, Toshinori Shimanouchi, Ryoichi Kuboi

BIOCHEMICAL ENGINEERING JOURNAL 46 ( 2 ) 154 - 160 2009年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE SA

The effect of negatively charged liposome on in vitro synthesis of a reporter protein, green fluorescent protein (UP), was investigated using a cell-free translation system. GFP was expressed with and without negatively charged liposome prepared with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylchorine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), resulting in the GFP fluorescence being reduced to approximately 60% in the presence of POPC/POPG at more than 30% POPG, depending oil its concentration. However, the amount of synthesized GFP products, as analyzed by SDS-PAGE, did not change with and without the POPC/POPG liposome. The results of the ultrafiltration operation indicate that the liposome interacts not only with synthesized polypeptide GFP but also with folded synthesized GFP (mature GFP). Liposome also inhibited refolding of unfolded GFP to its native state. The above results show that the POPC/POPG could inhibit the folding of GFP in the post-translational process of the gene expression product. (C) 2009 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.bej.2009.05.002

Polymethylthiophene/Nafion-modified glassy carbon electrode for selective detection of dopamine in the presence of ascorbic acid

Vu Thi Huong, Toshinori Shimanouchi, Do Phuc Quan, Hiroshi Umakoshi, Pham Hung Viet, Ryoichi Kuboi

JOURNAL OF APPLIED ELECTROCHEMISTRY 39 ( 10 ) 2035 - 2042 2009年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPRINGER

The possible use of an electrode modified with electroactive conductive poly(3-methylthiophene) (PMeT)/Nafion as a chemical sensor was investigated for the voltammetric analysis of Dopamine (DA). The electrochemical behavior of dopamine was examined by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. By using a PMeT-modified glassy carbon (GC/PMeT) electrode, DA and Ascorbic Acid (AA) signals could be separated but the AA at high concentrations still caused significant interference by overlapping the DA peak. In comparison to the GC/PMeT electrode, the glassy carbon (GC/Nafion/PMeT) electrode modified with hybrid film Nafion/PMeT was found to permit a superior separation by shifting the oxidation of AA peak toward the less positive potential. The DPV curves for a mixture of DA and AA at an GC/Nafion/PMeT electrode in a 0.1 M H(2)SO(4) solution showed peaks of DA and AA, at 0.45 and 0.21 V, respectively, indicating that the difference in the oxidation potential was 240 mV. In the 0.1 M H(2)SO(4) solution, the oxidation peak current on the differential pulse voltammograms for the GC/PMeT electrode increased linearly with the concentration of DA in the range 1 x 10(-6) to 1 x 10(-3) M, and the oxidation peak current on the differential pulse voltammograms for the GC/Nafion/PMeT electrode in the range 5 x 10(-7) to 2 x 10(-4) M. The DA detection sensitivity of the GC/Nafion/PMeT electrode (26.7 mu A mu M(-1) cm(-2)) was 22 times higher than that of the GC/PMeT electrode (1.21 mu A mu M(-1) cm(-2)).

DOI： 10.1007/s10800-009-9860-z

Preparation of Hollow Fiber Immobilized Liposome Membrane

Sugaya Hiroyuki, Umakoshi Hiroshi, Tohtake Yuji, OYAMA Ena, SHIMANOUCHI Toshinori, KUBOI Ryoichi

膜 34 ( 5 ) 272 - 280 2009年9月

記述言語：英語出版者・発行元：THE MEMBRANE SOCIETY OF JAPAN

Immobilized liposome membrane (ILM) was prepared in hollow fiber module (HF–ILM), where the liposome was loaded into the hollow fiber membrane module and was physically immobilized by forming the hydrogel in the cavity of the fiber membrane. Basic nature of the hydrogels prepared with xanthan gum (XG) and polyethyleneimine (PEI) via amino coupling method was first investigated in relation to the water contents of the gel matrix, so that the optimal condition for the liposome immobilization was obtained. The HF–ILM was prepared at the optimal condition, resulting in the high contents of immobilized liposome with high stability.

DOI： 10.5360/membrane.34.272

その他リンク： https://jlc.jst.go.jp/DN/JALC/00338580205?from=CiNii

Immobilization of intact liposomes on solid surfaces: A quartz crystal microbalance study

Thi Huong Vu, Toshinori Shimanouchi, Haruyuki Ishii, Hiroshi Umakoshi, Ryoichi Kuboi

JOURNAL OF COLLOID AND INTERFACE SCIENCE 336 ( 2 ) 902 - 907 2009年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE

Immobilization of model cell membranes (liposomes) on sensing devices is useful for the detection, evaluation, and analysis of interaction between cell membranes and proteins or other biological materials. The method to immobilize the liposomes on the solid surface was investigated by using the quartz crystal microbalance (QCM) method. focusing on the density of immobilized liposomes and their stability. The different liposomes were applied onto the planar surface of the QCM electrode by varying the immobilization method. The results showed that the immobilized liposomes depended not only on the immobilization method but also on the properties of the liposomes. The liposome with low membrane fluidity likely showed intact immobilization on a solid surface. An electrostatic interaction also affected the amount of immobilized liposomes on the functionalized quartz crystal. By using the amino-coupling method, intact liposomes could be immobilized on a solid surface and immobilized liposomes could stabilize for 10 h with frequency changes less than 5%. (C) 2009 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.jcis.2009.04.048

Inversion of the configuration of a single stereocenter in a β-heptapeptide leads to drastic changes in its interaction with phospholipid bilayers

Toshinori Shimanouchi, Peter Walde, James Gardiner, James Gardiner, Stefania Capone, Dieter Seebach, Ryochi Kuboi

ChemBioChem 10 ( 12 ) 1978 - 1981 2009年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

In summary, a change in the configuration of a single amino acid in a β3-heptapeptide can lead to remarkable changes in the physicochemical properties of the peptide with respect to the interaction with a (protein-free) biomimetic environment. Thus, the presence of an unnatural amino acid configuration in a peptide can have consequences not only with respect to specific interactions with proteins, but also, indirectly, with respect to the interaction with membrane lipids. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

DOI： 10.1002/cbic.200900175

Kinetic Study on Giant Vesicle Formation with Electroformation Method

Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

LANGMUIR 25 ( 9 ) 4835 - 4840 2009年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER CHEMICAL SOC

Giant vesicles (GVs) composed of zwitterionic phospholipids were prepared by the electroformation method. The growth behavior of GVs was quantitatively analyzed as a first-order kinetics of the radius of GVs to obtain the apparent growth rate constant k(Gr). On the basis of the dependence of the k(Gr) value on the preparation temperature and the lipid composition of GVs, the membrane fluidity of vesicle membranes was found to be dominant in the growth behavior of GVs. The comparison of the k(Gr) values with the membrane fluidity for GVs suggested the validity of the model that the swelling of the lipid membrane could induce the growth of GVs.

DOI： 10.1021/la8040488

Cationic Liposome Inhibits Gene Expression in an E. coli Cell-Free Translation System 査読

Bui, H. T, Umakoshi, H, Suga, K, Tanabe, T, Shimanouchi, T, Kuboi, R

Membrane 34 ( 3 ) 146 - 151 2009年3月

Liposome membrane can act like molecular and metal chaperones for oxidized and fragmented superoxide dismutase

Le Quoc Tuan, Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi

ENZYME AND MICROBIAL TECHNOLOGY 44 ( 2 ) 101 - 106 2009年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE INC

A mechanism for liposome-recruited activity of oxidized and fragmented superoxide dismutase (Fr.-SOD) [Tuan LQ, Umakoshi H, Shimanouchi T, Kuboi R. Liposome-recruited activity of oxidized and fragmented superoxide dismutase. Langmuir 2008;24:350-4] was further investigated, focusing on the secondary structure of Fr.-SOD. Liposome membrane was found to assist the conformational change of Fr.-SOD and reactivate the enzymatic activity. like molecular and metal chaperones. The loss of SOD activity and its secondary structure was observed during 6 h oxidation in 2 mM hydrogen peroxide. The contents of the alpha-helix and beta-sheet structures in the oxidized and fragmented SOD (2 mu M) were increased only in the presence of 10 mu M Cu2+ and Zn2+ together, or in the presence of 2 mM POPC liposomes. The mixture of all of these elements (fragmented SOD and POPC liposomes with Cu2+ and Zn2+) gave not only the increase of the alpha-helix and beta-sheet contents but also the mediation of the high SOD-like activity. (c) 2008 Published by Elsevier Inc.

DOI： 10.1016/j.enzmictec.2008.10.012

A Microbolometer of Bio-Thermochemical Sensor Immobilized Lipo some for Detection of Causative Protein of Alzheimer's Disease, Amyloid Beta

M. Noda, T. Asai, T. Shimanouchi, K. Yamashita, H. Umakoshi, M. Okuyama, R. Kuboi

PROCEEDINGS OF THE EUROSENSORS XXIII CONFERENCE 1 ( 1 ) 1071 - + 2009年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：ELSEVIER SCIENCE BV

Forming process of liposome droplet and its stable immobilization on a Si-based microbolometer are investigated and applied for detection of causative protein of Alzheimer's disease, amyloid beta (A beta) protein, where the disease is closely related to the amyloid fibril formation. This work is a preliminary but innovative approach applying a bio-thermochemical microsensor to monitor the formation. It is observed that the bolometer surface keeps a long time intact behavior of the liposome up to 24 hours. It seems from time course results with A beta that the variation in the temperature profile corresponds to that observed in fluorescence analysis result of the formation.

DOI： 10.1016/j.proche.2009.07.267

A Leakage Current Microsensor for Detection of Interaction between an Electrolyte-Entrapping Liposome and Protein

Minoru Noda, Takeshi Asai, Kaoru Yamashita, Toshinori Shimanouchi, Hiroshi Umakoshi, Masanori Okuyama, Ryouichi Kuboi

2009 IEEE SENSORS, VOLS 1-3 1883 - + 2009年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：IEEE

We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K-4[Fe(CN)(6)] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

DOI： 10.1109/ICSENS.2009.5398390

Characterization of Hollow Fiber Immobilized Liposome Membrane by Using Aqueous Two-Phase Partitioning Systems

Hiroyuki Sugaya, Hiroshi Umakoshi, Yuji Tohtake, Toshinori Shimanouchi, Ryoichi Kuboi

SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN 16 103 - 109 2009年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPAN ASSOC SOLVENT EXTRACTION

The modification of the surface of a polysulfone (PSf) membrane in a hollow fiber module was performed based on the evaluated hydrophobicity of the polymers used in its modification to develop a new type of membrane module immobilizing liposome. The surface net hydrophobicity of the polyvinylpyrrolidone (PVP), to be modified, was first characterized by using the aqueous two-phase partitioning method. The hollow fiber module using the PVP-modified PSf membrane was characterized based on the adsorption behaviors of standard amino acids, showing that the membrane surface was found to have a hydrophilic surface nearly equal to the difference in aqueous two-phase systems. The immobilized liposome membrane (ILM) in the above module was found to have a hydrophobic potential, thus promoting the adsorption of the hydrophobic molecules on its surface.

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