Updated on 2022/10/01

写真a

 
SHIMANOUCHI Toshinori
 
Organization
Faculty of Environmental and Life Science Associate Professor
Position
Associate Professor
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Degree

  • (BLANK) ( Osaka University )

Research Interests

  • 化学工学

  • Separation Science

  • Surface Science

Research Areas

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Transport phenomena and unit operations  / 晶析、反応分離、亜臨界水

  • Life Science / Biophysics  / 脂質膜、タンパク質の動的機能

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering  / バイオプロセスのためのソフトマター/ソフト界面設計

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Chemical reaction and process system engineering  / 環境プロセス設計、ハイブリッド触媒設計に基づくプロセス強化

Education

  • Osaka University     化学工学

    - 1998

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    Country: Japan

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  • Osaka University    

    - 1998

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  • Osaka University    

    - 1997

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  • Osaka University   基礎工学部   化学工学科

    - 1997

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    Country: Japan

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Research History

  • Okayama University   学術研究院 環境生命科学学域   Associate Professor

    2021.4

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    Country:Japan

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  • Okayama University   The Graduate School of Environmental and Life Science   Associate Professor

    2012.10 - 2021.3

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  • Nara National College of Technology

    2006 - 2007

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  • Osaka University   Graduate School of Engineering Science

    2001.1 - 2012.9

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Professional Memberships

Committee Memberships

  • 膜学会   編集委員  

    2017.4   

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  • 表面真空学会   ソフトマター部会庶務  

    2017.4   

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  • 分離技術会関西支部   幹事  

    2016.5   

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  • 表面真空学会   編集委員  

    2016.4   

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  • 生物工学会   関西支部委員  

    2002.4 - 2012.9   

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    Committee type:Academic society

    生物工学会

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Papers

  • Mechanisms of enhanced aggregation and fibril formation of Parkinson's disease-related variants of α-synuclein. International journal

    Takashi Ohgita, Norihiro Namba, Hiroki Kono, Toshinori Shimanouchi, Hiroyuki Saito

    Scientific reports   12 ( 1 )   6770 - 6770   2022.4

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    Aggregation of α-synuclein (α-syn) into amyloid fibrils is closely associated with Parkinson's disease (PD). Familial mutations or posttranslational truncations in α-syn are known as risk factor for PD. Here, we examined the effects of the PD-related A30P or A53T point mutation and C-terminal 123-140 or 104-140 truncation on the aggregating property of α-syn based on the kinetic and thermodynamic analyses. Thioflavin T fluorescence measurements indicated that A53T, Δ123‒140, and Δ104-140 variants aggregated faster than WT α-syn, in which the A53T mutation markedly increases nucleation rate whereas the Δ123‒140 or Δ104‒140 truncation significantly increases both nucleation and fibril elongation rates. Ultracentrifugation and western blotting analyses demonstrated that these mutations or truncations promote the conversion of monomer to aggregated forms of α-syn. Analysis of the dependence of aggregation reaction of α-syn variants on the monomer concentration suggested that the A53T mutation enhances conversion of monomers to amyloid nuclei whereas the C-terminal truncations, especially the Δ104-140, enhance autocatalytic aggregation on existing fibrils. In addition, thermodynamic analysis of the kinetics of nucleation and fibril elongation of α-syn variants indicated that both nucleation and fibril elongation of WT α-syn are enthalpically and entropically unfavorable. Interestingly, the unfavorable activation enthalpy of nucleation greatly decreases for the A53T and becomes reversed in sign for the C-terminally truncated variants. Taken together, our results indicate that the A53T mutation and the C-terminal truncation enhance α-syn aggregation by reducing unfavorable activation enthalpy of nucleation, and the C-terminal truncation further triggers the autocatalytic fibril elongation on the fibril surfaces.

    DOI: 10.1038/s41598-022-10789-6

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  • Sulfated glycosaminoglycans mediate prion-like behavior of p53 aggregates Reviewed International journal

    Naoyuki Iwahashi, Midori Ikezaki, Taro Nishikawa, Norihiro Namba, Takashi Ohgita, Hiroyuki Saito, Yoshito Ihara, Toshinori Shimanouchi, Kazuhiko Ino, Kenji Uchimura, Kazuchika Nishitsuji

    Proceedings of the National Academy of Sciences of the United States of America   117 ( 52 )   33225 - 33234   2021.12

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    Sulfated glycosaminoglycans (GAGs) such as heparan sulfate (HS) are heteropolysaccharides implicated in the pathology of protein aggregation diseases including localized and systemic forms of amyloidosis. Among subdomains of sulfated GAGs, highly sulfated domains of HS, called HS S-domains, have been highlighted as being critical for HS function in amyloidoses. Recent studies suggest that the tumor suppressor p53 aggregates to form amyloid fibrils and propagates in a prion-like manner; however, molecules and mechanisms that are involved in the prion-like behavior of p53 aggregates have not been addressed. Here, we identified sulfated GAGs as molecules that mediate prion-like behavior of p53 aggregates. Sulfated GAGs at the cell surface were required for cellular uptake of recombinant and cancer cell-derived p53 aggregates and extracellular release of p53 from cancer cells. We further showed that HS S-domains accumulated within p53 deposits in human ovarian cancer tissues, and enzymatic remodeling of HS S-domains by Sulf-2 extracellular sulfatase down-regulated cellular uptake of p53 aggregates. Finally, sulfated GAG-dependent cellular uptake of p53 aggregates was critical for subsequent extracellular release of the aggregates and gain of oncogenic function in recipient cells. Our work provides a mechanism of prion-like behavior of p53 aggregates and will shed light on sulfated GAGs as a common mediator of prions.

    DOI: 10.1073/PNAS.2009931117

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  • Fibril growth behavior of amyloid β on polymer-based planar membranes: Implications for the entanglement and hydration of polymers Reviewed

    Shimanouchi, M Iwamura, S Deguchi, Y Kimura

    Appl. Sci.   11 ( 10 )   4408   2021.7

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  • Quantitative Determination of Relative Permittivity Based on the Fluorescence Property of Pyrene Derivatives: An Interpretation of Hydrophobicity in Self-Assembled Aggregates of Nonionic Amphiphiles. Reviewed International journal

    Keita Hayashi, Haruna Sugimura, Toshiyuki Kamei, Toshinori Shimanouchi, Hidemi Nakamura, Hiroshi Umakoshi

    The journal of physical chemistry. B   125 ( 23 )   6192 - 6200   2021.6

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    Aggregates in aqueous solutions can embed hydrophobic molecules, and their interactions depend on the properties of the aggregates. The electric surface potential, molecular mobility, and gradual hydrophobicity are the properties that regulate the interactions, and it is essential to understand these to quantify the properties. Electric surface potential and molecular mobility are quantified using the zeta potential and NMR measurements. In this study, the quantification of gradual hydrophobicity within the aggregate based on the relative permittivity, also called the dielectric constant, has been estimated from fluorescence spectra of pyrene-dicarboxylic acid conjugates. The localization of the pyrene moiety was modified by conjugation with succinic acid, suberic acid, or dodecanedioic acid, and the conjugates were evaluated in the shallow, middle, and deep regions of the aggregates. Span and Tween surfactants have been employed to prepare these aggregates, because they form various kinds of aggregates such as micelles and vesicles. It was realized that the hydrophobicity gradually increased from the interface to the hydrophobic core. Alternatively, a comparison of hydrophobicity within the aggregates showed no remarkable difference. Moreover, the analyses suggested that there are a few water molecules in the deep region. These results support the idea of the localization of embedded molecules in aggregates.

    DOI: 10.1021/acs.jpcb.1c00170

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  • Microfluidic and hydrothermal preparation of vesicles using sorbitan monolaurate/polyoxyethylene (20) sorbitan monolaurate (Span 20/Tween 20). Reviewed International journal

    Toshinori Shimanouchi, Tetsuya Hayashi, Kazuki Toramoto, Saki Fukuma, Keita Hayashi, Kazuma Yasuhara, Yukitaka Kimura

    Colloids and surfaces. B, Biointerfaces   205   111836 - 111836   2021.5

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    Here, we present a method for preparing vesicles by combining hydrothermal emulsification with solvent diffusion (SD). The sorbitan monolaurate/polyoxyethylene (20) sorbitan monolaurate (Span 20/Tween 20) system was used as the target lipid because these lipids are cheap and advantageous for the production scale. The water-in-oil (W/O) emulsion stabilized with lipids was formed under hydrothermal conditions (240 °C under 10 MPa), followed by mixing with water that included lipids to obtain a W/O-in-water (W/O/W) emulsion. The SD for the W/O/W emulsion as a subsequent process yielded vesicles. The optimal preparation conditions were 50:50 wt% Span 20/Tween 20 as a mixing ratio (final lipid concentration 12 mM), octanoic acid as an organic solvent, 240 °C for 4 min during the hydrothermal treatment, and 4 °C for 24 h in the SD process. The diameter of the vesicles obtained was at most 100 nm, which was comparable to that of the W/O/W emulsion before SD. This suggested that the W/O/W emulsion acted as a template for vesicle formation. The number density, diameter, and membrane properties of vesicles depend on the mixing ratio of the water/oil/lipid system. Specifically, the number density of vesicles was low relative to that of vesicles prepared by the conventional method.

    DOI: 10.1016/j.colsurfb.2021.111836

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  • Characterization of water/organic biphasic system in liquid-liquid slug flow under hydrothermal conditions: Solvation, vorticity, and hydrophobicity Reviewed

    Toshinori Shimanouchi, Satoko Fujioka, Tatsuya Tanifuji, Kenta Yamamoto, Koichi Terasaka, Yukitaka Kimura

    Solvent Extraction Research and Development   28 ( 1 )   21 - 35   2021.4

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    The microcapillary was combined with the liquid-liquid slug flow to build the separation field. The partition behavior of seventeen kinds of substrates was investigated by using six kinds of water/organic biphasic systems under hydrothermal conditions (25 - 190° C at 10 MPa). The scale of hydrophobicity (HF) of water/organic biphasic system was estimated. The HF value for the biphasic system used here was comparable to the conventional aqueous two-phase systems and depended on the (de)solvation of substrates by water and organic solvent. Besides, the vortex field in the slug contributed to the enhanced mass transfer of substrates. Those results are available for the selection of water/organic biphasic system as the reactive separation of target materials.

    DOI: 10.15261/SERDJ.28.21

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  • バイセルのベシクルへの遷移法のための流体合流を用いたバイセルの連続調製 Reviewed

    SungHak Choi, BongSu Kang, Toshinori Shimanouchi, Keesung Kim, HoSup Jung

    9 ( 7 )   2021

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  • Label-free, chronological and selective detection of aggregation and fibrillization of amyloid β protein in serum by microcantilever sensor immobilizing cholesterol-incorporated liposome Reviewed International journal

    Tomoya Taniguchi, Toshinori Shimanouchi, Masayuki Sohgawa, Minoru Noda

    Biotechnology and Bioengineering   117 ( 8 )   2469 - 2478   2020.8

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    To facilitate the early diagnosis of Alzheimer's disease and mild cognitive impairment patients, we developed a cantilever-based microsensor that immobilized liposomes of various phospholipids to detect a trace amount of amyloid β (Aβ) protein, and investigated its aggregation and fibrillization on model cell membranes in human serum. Three species of liposomes composed of different phospholipids of 1,2-dipalmtoyl-sn-glycero-3-phosphocholine (DPPC), DPPC/phosphatidyl ethanolamine and 1,2-dipalmitoyl-sn-glycero-3-phosphorylglycerol having varied hydrophilic groups were applied, which showed different chronological interactions with Aβ(1–40) protein and varied sensitivities of the cantilever sensor, depending on their specific electrostatic charged conditions, hydrophilicity, and membrane fluidity. 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) having short hydrophobic carbon chains confirmed to show a large interaction with Aβ(1–40) and a high sensitivity. Furthermore, the incorporation of cholesterol into DMPC was effective to selectively detect Aβ(1–40) in human serum, which effect was also checked by quartz crystal microbalance. Finally, Aβ detection of 100-pM order was expected selectively in the serum by using the developed biosensor.

    DOI: 10.1002/bit.27380

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/bit.27380

  • Phosphatidylethanolamine accelerates aggregation of the amyloidogenic N-terminal fragment of apoA-I Reviewed International journal

    Naoko Kurimitsu, Chiharu Mizuguchi, Kaho Fujita, Suzuno Taguchi, Takashi Ohgita, Kazuchika Nishitsuji, Toshinori Shimanouchi, Hiroyuki Saito

    FEBS Letters   594 ( 9 )   1443 - 1452   2020.5

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    Membrane lipid composition is known to influence aggregation and fibril formation of many amyloidogenic proteins. Here, we found that phosphatidylethanolamine (PE) accelerates aggregation of the N-terminal 1‒83 fragment of an amyloidogenic G26R variant of apoA-I on lipid membranes. Circular dichroism and isothermal titration calorimetry measurements demonstrated that PE does not affect the α-helical structure and lipid binding property of apoA-I 1-83/G26R. Rather, fluorescence measurements indicated that PE induces more ordered lipid packing at the interfacial and acyl chain regions, providing more hydrophobic environments especially around the highly amyloidogenic regions in apoA-I on the membrane surface. These results suggest that PE promotes aggregation of the amyloidogenic N-terminal fragment of apoA-I on lipid membranes by inducing hydrophobic membrane environments.

    DOI: 10.1002/1873-3468.13737

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  • Analysis of the Partitioning Behavior of Horseradish Peroxidase to Phospholipid and Surfactant Membranes Reviewed

    Saki Fukuma, Toshinori Shimanouchi, Kazuma Yasuhara, Yukitaka Kimura

    Solvent Extraction Research and Development   27 ( 2 )   113 - 213   2020

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    The partitioning behavior of horseradish peroxidase (HRP) to phospholipid- and surfactant vesicles has been analyzed. Peripheral binding of the zwitterionic phospholipid vesicle membranes is proposed with insertion into the membrane interior of negatively charged surfactant vesicles, resulting from non-electrostatic interaction. The insertion of HRP is interpreted by considering the interaction between headgroups of phospholipid and between headgroups of surfactant.

    DOI: 10.15261/serdj.27.113

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  • Application of liposome membrane as the reaction field: A case study using the Horner–Wadsworth–Emmons reaction Reviewed

    Toshinori Shimanouchi, Yuki Kitagawa, Yukitaka Kimura

    Journal of Bioscience and Bioengineering   128 ( 2 )   198 - 202   2019.8

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    The properties of the liposome membrane as a reaction field were investigated by focusing on the Horner–Wadsworth–Emmons reaction as a case study. Use of the liposomes existing in the gel phase resulted in the enhanced activity of the substrates and furnished the products with same E/Z stereoselectivity as in the liposome-free system. The membrane environment in the gel phase most likely assisted the formation of adducts that induced selective generation of the E-isomer. The possible role of liposomes is to assist the proton removal from the reactant, rather than providing the basic interfacial environment.

    DOI: 10.1016/j.jbiosc.2019.01.015

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  • Alteration of Membrane Physicochemical Properties by Two Factors for Membrane Protein Integration Reviewed International journal

    K. Nomura, Toshiyuki Yamaguchi, S. Mori, K. Fujikawa, Ken ichi Nishiyama, Toshinori Shimanouchi, Yasushi Tanimoto, Kenichi Morigaki, K. Shimamoto

    Biophysical Journal   117 ( 1 )   99 - 110   2019.7

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    After a nascent chain of a membrane protein emerges from the ribosomal tunnel, the protein is integrated into the cell membrane. This process is controlled by a series of proteinaceous molecular devices, such as signal recognition particles and Sec translocons. In addition to these proteins, we discovered two endogenous components regulating membrane protein integration in the inner membrane of Escherichia coli. The integration is blocked by diacylglycerol (DAG), whereas the blocking is relieved by a glycolipid named membrane protein integrase (MPIase). Here, we investigated the influence of these integration-blocking and integration-promoting factors on the physicochemical properties of membrane lipids via solid-state NMR and fluorescence measurements. These factors did not have destructive effects on membrane morphology because the membrane maintained its lamellar structure and did not fuse in the presence of DAG and/or MPIase at their effective concentrations. We next focused on membrane flexibility. DAG did not affect the mobility of the membrane surface, whereas the sugar chain in MPIase was highly mobile and enhanced the flexibility of membrane lipid headgroups. Comparison with a synthetic MPIase analog revealed the effects of the long sugar chain on membrane properties. The acyl chain order inside the membrane was increased by DAG, whereas the increase was cancelled by the addition of MPIase. MPIase also loosened the membrane lipid packing. Focusing on the transbilayer movement, MPIase reduced the rapid flip-flop motion of DAG. On the other hand, MPIase could not compensate for the diminished lateral diffusion by DAG. These results suggest that by manipulating the membrane lipids dynamics, DAG inhibits the protein from contacting the inner membrane, whereas the flexible long sugar chain of MPIase increases the opportunity for interaction between the membrane and the protein, leading to membrane integration of the newly formed protein.

    DOI: 10.1016/j.bpj.2019.05.014

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  • Temperature Measurement by Sublimation Rate as a Process Analytical Technology Tool in Lyophilization Reviewed International journal

    Hidenori Kawasaki, Toshinori Shimanouchi, Hiroyuki Sawada, Hiroshi Hosomi, Yuta Hamabe, Yukitaka Kimura

    Journal of Pharmaceutical Sciences   108 ( 7 )   2305 - 2314   2019.7

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    Product temperature (T ) and drying time constitute critical material attributes and process parameters in the lyophilization process and especially during the primary drying stage. In the study, we performed a temperature measurement by the sublimation rate (TMbySR) to monitor the T value and determine the end point of primary drying. First, the water vapor transfer resistance coefficient through the main pipe from the chamber to the condenser (C ) was estimated via the water sublimation test. The use of C value made it possible to obtain the time course of T from the measurement of pressure at the drying chamber and at the condenser. Second, a Flomoxef sodium bulk solution was lyophilized by using the TMbySR system. The outcome was satisfactory when compared with that obtained via conventional sensors. The same was applicable for the determination of the end point of primary drying. A laboratory-scale application of the TMbySR system was evidenced via the experiment using 220-, 440-, and 660-vial scales of lyophilization. The outcome was not dependent on the loading amount. Thus, the results confirmed that the TMbySR system is a promising tool in laboratory scale. b b r r b

    DOI: 10.1016/j.xphs.2019.02.015

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  • Kinetic pH Titration to Predict the Acid and Hydrothermal Conditions for the Hydrolysis of Disaccharides: Use of a Microcapillary System Reviewed

    Toshinori Shimanouchi, Ryota Mano, Yu Yoshioka, Ayaka Fukuda, Kyung Min Park, Yukitaka Kimura

    Journal of Chemistry   2019   3985915   2019

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    The hydrolysis of disaccharides was conducted using a microcapillary system under hydrothermal conditions (up to 190°C at 10 MPa and pH 4-11). The hydrolysis reaction showed a sigmoidal progression with time, especially under alkaline conditions. Analysis using a kinetic model yielded the reaction induction period. The specific pH value (pH ) at the induction time, which is the pH value corresponding to the progression of disaccharide hydrolysis, was peculiar to each disaccharide. Finally, the calculation of the electron density around the oxygen atom of the glycosidic bond between saccharides was found to roughly predict the pH value required for the progression of hydrolysis. amb amb

    DOI: 10.1155/2019/3985915

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  • Recent Development of Optimization of Lyophilization Process Reviewed

    Hidenori Kawasaki, Toshinori Shimanouchi, Yukitaka Kimura

    Journal of Chemistry   2019   2019

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    The objective of this review is to survey the development of the optimization of lyophilization. The optimization study of the lyophilizer has been roughly developing by the order of (i) trial-and-error approach, (ii) process modeling using mathematical models, (iii) scalability, and (iv) quality-by-design. From the conventional lyophilization studies based on the trial-and-error, the key parameters to optimize the operation of lyophilization were found out, i.e., critical material attributes (CMAs), critical process parameters (CPPs), and critical quality attributes (CQAs). The mathematical models using the key parameters mentioned above have been constructed from the viewpoints of the heat and mass transfer natures. In many cases, it is revealed that the control of the primary drying stage determines the outcome of the lyophilization of products, as compared with the freezing stage and the secondary drying stage. Thus, the understanding of the lyophilization process has proceeded. For the further improvement of the time and economical cost, the design space is a promising method to give the possible operation range for optimizing the lyophilization operation. This method is to search the optimized condition by reducing the number of key parameters of CMAs, CPPs, and CQAs. Alternatively, the transfer of lyophilization recipe among the lab-, pilot-, and production-scale lyophilizers (scale-up) has been examined. Notably, the scale-up of lyophilization requires the preservation of lyophilization dynamics between the two scales, i.e., the operation of lab- or pilot-scale lyophilizer under HEPA-filtrated airflow condition. The design space determined by focusing on the primary drying stage is large and involves the undesired variations in the quality of final products due to the heterogeneous size distribution of ice crystals. Accordingly, the control of the formation of the ice crystal with large size gave impact on the product quality and the productivity although the large water content in the final product should be improved. Therefore, the lyophilization should take into account the quality by design (QbD). The monitoring method of the quality of the product in lyophilization process is termed the "process analytical technology (PAT)." Recent PAT tools can reveal the lyophilization dynamics to some extent. A combination of PAT tools with a model/scale-up theory is expected to result in the QbD, i.e., a quality/risk management and an in situ optimization of lyophilization operation.

    DOI: 10.1155/2019/9502856

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  • Innovative Strategies and Emerging Technologies for Food Safety Reviewed

    Kyung Min Park, Sang Hyun Park, Toshinori Shimanouchi

    Journal of Chemistry   2019   2019

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    DOI: 10.1155/2019/4276426

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  • Structural and physicochemical characteristics of granular malic acid-treated sweet potato starch containing heat-stable resistant starch Reviewed

    Chinwoo Kwon, Ha Ram Kim, Tae Wha Moon, Seung Hyun Lee, Chang Joo Lee, Toshinori Shimanouchi

    Journal of Chemistry   2019   2019

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    This study investigated the structural and physicochemical characteristics of malic acid-treated sweet potato starch. Sweet potato starch mixed with various concentrations of malic acid solution underwent either thermal or nonthermal treatment. Observation of samples under a light microscope ensured the maintenance of granular shape and the Maltese cross. FT-IR spectra displayed a distinct carbonyl peak at 1722 cm , and analysis of the degree of substitution (DS) indicated an increase in the extent of ester bonds with increasing concentrations of malic acid. The DS of 2.0M-130 (0.214) was the highest and that of 0.5M-130 was the lowest (0.088) among the reacted starches. In vitro digestion test revealed an increased amount of resistant starch when a high concentration of malic acid was used. In addition, thermally treated samples maintained a higher content of resistant starch (RS) after 30 min of cooking at 100°C. After cooking, 2.0M-130 had an RS fraction of 53.4% which was reduced to 49.9% after cooking, revealing greater heat stability compared with nonthermally treated samples. The structure of malic acid-treated starch was investigated using a differential scanning calorimeter (DSC), an X-ray diffractometer, a rapid visco analyzer (RVA), and analysis of apparent amylose content. The results showed that thermal and malic acid treatment of starch caused not only partial hydrolysis but also rearrangement of the crystalline area and helix structure of starch by esterification. Analysis of malic acid-treated starch, using a rapid visco analyzer showed no pasting properties, due to lack of its swelling caused by the malic acid cross link. -1

    DOI: 10.1155/2019/2903252

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  • Effect of phosphatidylserine and cholesterol on membrane-mediated fibril formation by the N-terminal amyloidogenic fragment of apolipoprotein A-I Reviewed International journal

    Chiharu Mizuguchi, Mitsuki Nakamura, Naoko Kurimitsu, Takashi Ohgita, Kazuchika Nishitsuji, Teruhiko Baba, Akira Shigenaga, Toshinori Shimanouchi, Keiichiro Okuhira, Akira Otaka, Hiroyuki Saito

    Scientific Reports   8 ( 1 )   5497 - 5497   2018.12

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    Here, we examined the effects of phosphatidylserine (PS) and cholesterol on the fibril-forming properties of the N-terminal 1-83 fragment of an amyloidogenic G26R variant of apoA-I bound to small unilamellar vesicles. A thioflavin T fluorescence assay together with microscopic observations showed that PS significantly retards the nucleation step in fibril formation by apoA-I 1-83/G26R, whereas cholesterol slightly enhances fibril formation. Circular dichroism analyses demonstrated that PS facilitates a structural transition from random coil to α-helix in apoA-I 1-83/G26R with great stabilization of the α-helical structure upon lipid binding. Isothermal titration calorimetry measurements revealed that PS induces a marked increase in capacity for binding of apoA-I 1-83/G26R to the membrane surface, perhaps due to electrostatic interactions of positively charged amino acids in apoA-I with PS. Such effects of PS to enhance lipid interactions and inhibit fibril formation of apoA-I were also observed for the amyloidogenic region-containing apoA-I 8-33/G26R peptide. Fluorescence measurements using environment-sensitive probes indicated that PS induces a more solvent-exposed, membrane-bound conformation in the amyloidogenic region of apoA-I without affecting membrane fluidity. Since cell membranes have highly heterogeneous lipid compositions, our findings may provide a molecular basis for the preferential deposition of apoA-I amyloid fibrils in tissues and organs.

    DOI: 10.1038/s41598-018-23920-3

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  • Design of Pyrene-Fatty Acid Conjugates for Real-Time Monitoring of Drug Delivery and Controllability of Drug Release Reviewed International journal

    Keita Hayashi, Yuma Mitsuyoshi, Toshiyuki Kamei, Toshinori Shimanouchi, Keishi Suga, Yukihiro Okamoto, Hidemi Nakamura, Hiroshi Umakoshi

    ACS Omega   3 ( 3 )   3572 - 3580   2018.3

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    Fluorescence probes are usually employed to analyze pharmacokinetics of drug carriers; however, this method using usual probes is not suitable to monitor drug carriers in detail because fluorescence spectra do not change by the disruption of drug carriers. In this study, pyrene-fatty acid conjugates were investigated as probes to monitor the state of drug carriers in real time. 1-Pyrenemethanol was conjugated with fatty acids, such as lauric acid, stearic acid, and behenic acid, and the conjugates were stirred in ethanol, resulting in the formation of submicron particles; these particles exhibited excimer emission. When J774.1 and Colon 26 cells were treated with these particles, the associated fluorescence spectra shifted from excimer emission to monomer emission. Moreover, the degree of change was controlled by the type of fatty acid. These results support the design of drug carriers that can be used to monitor pharmacokinetics in real time and to control the disruption time.

    DOI: 10.1021/acsomega.7b02061

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  • Scale-up procedure for primary drying process in lyophilizer by using the vial heat transfer and the drying resistance Reviewed

    Hidenori Kawasaki, Toshinori Shimanouchi, Masaharu Yamamoto, Kanako Takahashi, Yukitaka Kimura

    Chemical and Pharmaceutical Bulletin   66 ( 11 )   1048 - 1056   2018

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    The objective of this study is to design primary drying conditions in a production lyophilizer based on a pilot lyophilizer. Although the shelf temperature and the chamber pressure need to be designed to maintain the sublimation interface temperature of the formulation below the collapse temperature, it is difficult to utilize a production lyophilizer to optimize cycle parameters for manufacturing. In this report, we assumed that the water vapor transfer resistance (Rp) in the pilot lyophilizer can be used in the commercial lyophilizer without any correction, under the condition where both lyophilizers were operated in the high efficiency particulate air (HEPA)-filtrated airflow condition. The shelf temperature and the drying time for the commercial manufacturing were designed based on the maximum Rp value calculated from the pilot lyophilizer (1008 vials) under HEPA-filtrated airflow condition and from the vial heat transfer coefficient of the production lyophilizer (6000 vials). And, the cycle parameters were verified using the production lyophilizer of 60000 vials. It was therefore concluded that the operation of lab- or pilot-scale lyophilizer under HEPA-filtrated airflow condition was one of important factors for the scale-up.

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  • Effect of controlled nucleation of ice crystals on the primary drying stage during lyophilization Reviewed

    Hidenori Kawasaki, Toshinori Shimanouchi, Kanako Takahashi, Yukitaka Kimura

    Chemical and Pharmaceutical Bulletin   66 ( 12 )   1122 - 1130   2018

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    The freezing stage cannot be directly controlled, which leads to variation in product quality and low productivity during the lyophilization process. Our objective was to establish a robust design space for the primary drying stage using ice nucleation control based on the pressurization and depressurization technique. We evaluated the specific surface area (SSA), water content, scanning electron microscopy (SEM) images, and water vapor transfer resistance of the dried layer (R ) of the products. The ice nucleation control resulted in a reduction of the SSA value and in an increase in water content. SEM observation suggested that the ice nucleation control enabled formation of large ice crystals, which was consistent with the reduction in the R value. As a result, the generation of collapsed cakes was inhibited, whereas 18% of the collapsed cakes were observed without ice nucleation control. Finally, this technique succeeded in determining a robust design space for the primary drying stage to produce uniform products of higher productivity. It was considered, from the present findings, that controlling the formation of large ice crystals impacted the product quality and productivity. p p

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  • A label-free fluorescent array sensor utilizing liposome encapsulating calcein for discriminating target proteins by principal component analysis International journal

    Ryota Imamura, Naoki Murata, Toshinori Shimanouchi, Kaoru Yamashita, Masayuki Fukuzawa, Minoru Noda

    Sensors (Switzerland)   17 ( 7 )   1630   2017.7

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    A new fluorescent arrayed biosensor has been developed to discriminate species and concentrations of target proteins by using plural different phospholipid liposome species encapsulating fluorescent molecules, utilizing differences in permeation of the fluorescent molecules through the membrane to modulate liposome-target protein interactions. This approach proposes a basically new label-free fluorescent sensor, compared with the common technique of developed fluorescent array sensors with labeling. We have confirmed a high output intensity of fluorescence emission related to characteristics of the fluorescent molecules dependent on their concentrations when they leak from inside the liposomes through the perturbed lipid membrane. After taking an array image of the fluorescence emission from the sensor using a CMOS imager, the output intensities of the fluorescence were analyzed by a principal component analysis (PCA) statistical method. It is found from PCA plots that different protein species with several concentrations were successfully discriminated by using the different lipid membranes with high cumulative contribution ratio. We also confirmed that the accuracy of the discrimination by the array sensor with a single shot is higher than that of a single sensor with multiple shots.

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  • Tailor-made drug carrier: Comparison of formation-dependent physicochemical properties within self-assembled aggregates for an optimal drug carrier International journal

    Keita Hayashi, Hideka Iwai, Toshiyuki Kamei, Kaede Iwamoto, Toshinori Shimanouchi, Sakiko Fujita, Hidemi Nakamura, Hiroshi Umakoshi

    Colloids and Surfaces B: Biointerfaces   152   269 - 276   2017.4

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    Self-assembled surfactant aggregates, such as micelles and vesicles, have been investigated for their application as drug carriers in the treatment of various diseases. However, the characteristics that decide which aggregate is the best drug carrier for each disease have not yet been clarified. In order to design an optimal drug carrier for each disease, various kinds of self-assembled aggregates, such as spherical micelles, lens-like vesicles, and tube-like vesicles, were evaluated by “multiple techniques” including dynamic light scattering, differential scanning calorimetry, nuclear magnetic resonance spectroscopy, and fluorescence measurement using the Laurdan probe. These studies led to the compilation of a database on the formation-dependent properties of self-assembled aggregates. As the relationship between physicochemical properties of self-assembled aggregates and their functions as drug carriers have been extensively reported, this database can be utilized for designing an optimal drug carrier, i.e., a tailor-made drug carrier.

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  • Effect of heparin on amyloid fibril formation of apoA-I fragment peptides Reviewed

    Shiho Mikawa, Chiharu Mizuguchi, Izumi Morita, Hiroyuki Oyama, Teruhiko Baba, Akira Shigenaga, Toshinori Shimanouchi, Norihiro Kobayashi, Akira Otaka, Kenichi Akaji, Hiroyuki Saito

    Peptide Science 2016   149 - 151   2017.2

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  • Calcein leakage behavior from vesicles induced by proteinvesicle interaction: A study by surface pressurearea isotherms

    Saki Fukuma, Toshinori Shimanouchi, Keita Hayashi, Yukitaka Kimura

    Chemistry Letters   46 ( 7 )   1036 - 1039   2017

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    The release behavior of substrates encapsulated in the vesicle can be controlled by the interaction between the vesicular membrane and the protein. However, its control mechanism is still now unknown. Therefore, the proteinvesicle interaction was investigated by a A isotherm. It was clarified that the released amount of encapsulated substrates increased with increasing proteinvesicle interaction. This result leads to the development of drug delivery systems (DDS) and of biosensor technology.

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  • Effect of wet and dry torrefaction process on fuel properties of solid fuels derived from bamboo and Japanese cedar

    Wei Yang, Shengji Wu, Hui Wang, Pengyu Ma, Toshiniri Shimanouchi, Yukitaka Kimura, Jie Zhou

    BioResources   12 ( 4 )   8629 - 8640   2017

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    Torrefaction is a promising pretreatment process to convert biomass into high energy density solid fuel for further thermal conversion systems. In this study, the effects of wet and dry torrefaction on the properties of solid fuels prepared from bamboo and Japanese cedar were investigated in a batch reactor. The yields of solid fuels decreased with increasing treatment temperature in both torrefaction processes, mainly due to the decomposition of cellulose and hemicellulose. Cellulose showed higher reactivity than hemicellulose in both biomasses. The higher heating values (HHV) of solid fuels prepared at the treatment temperatures higher than 240 °C in both torrefaction processes reached the same level as those of commercial coals. Wet torrefaction was better than dry torrefaction for decomposing bamboo and Japanese cedar. Dry torrefaction had more favorable impact than wet torrefaction on improving the fuel properties of bamboo and Japanese cedar because of its lower energy input, higher solid fuel yield, higher energy yield, and similar HHV under the same conditions. The crystalline structure of solid fuel had no great change below 260 °C in both torrefaction processes and was completely destroyed at 300 °C during dry torrefaction.

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  • The structural evaluation of amyloid beta on lipid membranes

    Satoka Aoyagi, Miki Iwamura, Toshinori Shimanouchi, Yuta Yokoyama, Hideo Iwai

    Surface and Interface Analysis   48 ( 11 )   1096 - 1099   2016.11

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    Amyloid beta (Aβ) adsorption onto lipid membranes depending on the condition of a lipid was investigated by means of time-of-flight secondary ion mass spectrometry (ToF-SIMS). Aβ aggregation depending on the different hardness of lipid membrane has not been clarified yet although it is important to understand Alzheimer's disease. Aβ (1–40) on three different lipid membranes having different transition temperatures has been evaluated using ToF-SIMS in the previous study, and in this study, the differences between Aβ on liquid crystalline-phase lipid membranes and that on gel-phase lipid membranes were investigated in order to clarify the mechanisms of aggregation and peptide folding change. ToF-SIMS secondary ion images clearly showed Aβ distribution on lipid membranes. Because ToF-SIMS data is extremely complicated although it contains rich information, it was analysed by principal component analysis (PCA). Score images indicated by PCA are consistent with the images of secondary ions related to Aβ and are clearer than the secondary ion images. Moreover, PCA results suggest the difference between Aβ on different lipid membranes in terms of amino acid fragment ions, and the orientation of Aβ on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine was indicated. Copyright © 2016 John Wiley & Sons, Ltd.

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  • Heparin promotes fibril formation by the N-terminal fragment of amyloidogenic apolipoprotein A-I. International journal

    Shiho Mikawa, Chiharu Mizuguchi, Kazuchika Nishitsuji, Teruhiko Baba, Akira Shigenaga, Toshinori Shimanouchi, Naomi Sakashita, Akira Otaka, Kenichi Akaji, Hiroyuki Saito

    FEBS letters   590 ( 20 )   3492 - 3500   2016.10

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    Glycosaminoglycans are known to be associated with extracellular amyloid deposits of various amyloidogenic proteins. In this study, we found that the glycosaminoglycan heparin greatly accelerates the elongation step in fibril formation by the N-terminal 1-83 fragment of human apolipoprotein A-I (apoA-I), especially in the amyloidogenic W50R variant. Using fragment peptides, we demonstrate that heparin significantly promotes β-transition and fibril formation of the highly amyloidogenic region spanning residues 44-65 and colocalizes with fibrils formed by the W50R variant. These results suggest the possible role of glycosaminoglycans in fibril formation by amyloidogenic apoA-I variants.

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  • ToF-SIMS analysis of amyloid beta aggregation on different lipid membranes. International journal

    Yuta Yokoyama, Satoka Aoyagi, Toshinori Shimanouchi, Miki Iwamura, Hideo Iwai

    Biointerphases   11 ( 2 )   02A314 - 6   2016.6

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    Amyloid beta (Aβ) peptides are considered to be strongly related to Alzheimer's disease. Aβ peptides form a β-sheet structure on hard lipid membranes and it would aggregate to form amyloid fibrils, which are toxic to cells. However, the aggregation mechanism of Aβ is not fully understood. To evaluate the influence of the lipid membrane condition for Aβ aggregation, the adsorption forms of Aβ (1-40) on mixture membranes of lipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and cholesterol β-d-glucoside (β-CG) were investigated by time-of-flight secondary ion mass spectrometry. As a result, Aβ adsorbed along the localized DMPC lipid on the mixture lipid membranes, whereas it was adsorbed homogeneously on the pure DMPC and β-CG membranes. Moreover, amino acid fragments that mainly existed in the n-terminal of Aβ (1-40) peptide were strongly detected on the localized DMPC region. These results suggested that the Aβ was adsorbed along the localized DMPC lipid with a characteristic orientation. These findings suggest that the hardness of the membrane is very sensitive to coexisting materials and that surface hardness is important for aggregation of Aβ.

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  • Chemical Conversion and Liquid-Liquid Extraction of 5-Hydroxymethylfurfural from Fructose by Slug Flow Microreactor

    Toshinori Shimanouchi, Yoshitaka Kataoka, Tatsuya Tanifuji, Yukitaka Kimura, Satoko Fujioka, Koichi Terasaka

    AICHE JOURNAL   62 ( 6 )   2135 - 2143   2016.6

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    A dehydration of fructose in the water/methyl isobuthyl ketone (MIBK) biphasic system can yield 5hydroxymethylfulfural (IIMF) to be successfully extracted into the organic MIBK phase. The [IMF production and yield in MIRK phase was discussed by using a simplified model taking into consideration of the slug flow. The extraction resistance of IIMF across the interface between water and MIRK depended on the line velocity and the flow rate ratio. It was likely that the velocity field generated in the slug flow contributed to an increase in the mass transfer of IIMF. 2016 American Institute of Chemical Engineers AIChE J, 62: 2135-2143, 2016

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  • Chemical Conversion and Liquid-Liquid Extraction of 5-Hydroxymethylfurfural from Fructose by Slug Flow Microreactor

    Toshinori Shimanouchi, Yoshitaka Kataoka, Tatsuya Tanifuji, Yukitaka Kimura, Satoko Fujioka, Koichi Terasaka

    AICHE JOURNAL   62 ( 6 )   2135 - 2143   2016.6

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    A dehydration of fructose in the water/methyl isobuthyl ketone (MIBK) biphasic system can yield 5hydroxymethylfulfural (IIMF) to be successfully extracted into the organic MIBK phase. The [IMF production and yield in MIRK phase was discussed by using a simplified model taking into consideration of the slug flow. The extraction resistance of IIMF across the interface between water and MIRK depended on the line velocity and the flow rate ratio. It was likely that the velocity field generated in the slug flow contributed to an increase in the mass transfer of IIMF. 2016 American Institute of Chemical Engineers AIChE J, 62: 2135-2143, 2016

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  • Detection of Fibrillization Process of Amyloid Beta Protein Using Arrayed Biosensor with Liposome Encapsulating Fluorescent Molecules

    R. Imamura, T. Shimanouchi, N. Murata, K. Yamashita, M. Fukuzawa, M. Noda

    PROCEEDINGS OF THE 30TH ANNIVERSARY EUROSENSORS CONFERENCE - EUROSENSORS 2016   168   1414 - 1417   2016

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    We have successfully detected fibrillization process of amyloid-beta(1-40) protein (A beta(1-40)) as causative compounds of Alzheimer's disease by our developed bio array sensing system utilizing different phospholipid liposomes encapsulating fluorescent molecules. The fluorescent intensity is dependent on the leakage of fluorescent molecules through perturbed lipid membrane of liposome. Therefore, the intensity considerably depends on the liposome-A beta interaction strength on fibrillization process of A beta(1-40). It is noted that the behavior of the fluorescent intensity corresponded to that of our cantilever sensor when measuring the interaction. Moreover, difference in A beta(1-40) concentration or liposome-A beta(1-40) interaction strength was successfully discriminated by principal component analysis. Consequently, we believe that the fluorescent liposome sensor is very effective for discriminating aggregation and fibrillization processes and concentrations of A beta(1-40). (C) 2016 Published by Elsevier Ltd.

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  • Formation of lens-like vesicles induced via microphase separations on a sorbitan monoester membrane with different headgroups. International journal

    Keita Hayashi, Hideka Iwai, Toshinori Shimanouchi, Hiroshi Umakoshi, Tomoyuki Iwasaki, Ayako Kato, Hidemi Nakamura

    Colloids and surfaces. B, Biointerfaces   135   235 - 242   2015.11

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    The microphase separation of lipid molecules on a vesicle membrane can be induced, depending on the difference in the geometric structures of their headgroups. Through cryo-transmission-electron-microscopy analysis, a lens-like vesicle was prepared by mixing 50 wt% Span 40 (sorbitan monopalmitate) and 50 wt% Tween 40 [polyoxyethylene (20) sorbitan monopalmitate]. Considering the molecular structures of Span 40 and Tween 40, the high-curvature region was mainly formed by Tween 40. As determined by Fourier-transform infrared spectroscopy, dielectric-dispersion analysis, and differential scanning calorimetry, a hydration layer was likely formed because polyoxyethylene conjugates with the headgroups of Tween 40. These investigations of the obtained self-assembled aggregates of nonionic surfactants with heterogeneous surfaces could contribute to the development of new types of biomaterials.

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  • Amyloidogenic Mutation Promotes Fibril Formation of the N-terminal Apolipoprotein A-I on Lipid Membranes. International journal

    Chiharu Mizuguchi, Fuka Ogata, Shiho Mikawa, Kohei Tsuji, Teruhiko Baba, Akira Shigenaga, Toshinori Shimanouchi, Keiichiro Okuhira, Akira Otaka, Hiroyuki Saito

    The Journal of biological chemistry   290 ( 34 )   20947 - 20959   2015.8

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    The N-terminal amino acid 1-83 fragment of apolipoprotein A-I (apoA-I) has a strong propensity to form amyloid fibrils at physiological neutral pH. Because apoA-I has an ability to bind to lipid membranes, we examined the effects of the lipid environment on fibril-forming properties of the N-terminal fragment of apoA-I variants. Thioflavin T fluorescence assay as well as fluorescence and transmission microscopies revealed that upon lipid binding, fibril formation by apoA-I 1-83 is strongly inhibited, whereas the G26R mutant still retains the ability to form fibrils. Such distinct effects of lipid binding on fibril formation were also observed for the amyloidogenic prone region-containing peptides, apoA-I 8-33 and 8-33/G26R. This amyloidogenic region shifts from random coil to α-helical structure upon lipid binding. The G26R mutation appears to prevent this helix transition because lower helical propensity and more solvent-exposed conformation of the G26R variant upon lipid binding were observed in the apoA-I 1-83 fragment and 8-33 peptide. With a partially α-helical conformation induced by the presence of 2,2,2-trifluoroethanol, fibril formation by apoA-I 1-83 was strongly inhibited, whereas the G26R variant can form amyloid fibrils. These findings suggest a new possible pathway for amyloid fibril formation by the N-terminal fragment of apoA-I variants: the amyloidogenic mutations partially destabilize the α-helical structure formed upon association with lipid membranes, resulting in physiologically relevant conformations that allow fibril formation.

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  • Surface properties of woody thin boards composed of commercially available lignin and cellulose: Relationship between the orientation of lignin and water repellency

    Toshinori Shimanouchi, Tomoya Kamba, Wei Yang, Satoka Aoyagi, Yukitaka Kimura

    APPLIED SURFACE SCIENCE   347   406 - 413   2015.8

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    Woody thin boards were prepared from lignin, cellulose, and water by compression molding at 180 degrees C and 25 MPa for 10 min. Boards with higher contact angles gave lower values of relative permittivity on their surface. Attenuated-total reflection Fourier transfer infrared spectroscopy suggested that more lignin existed on the surface of the boards with the high contact angle, which was also supported by scanning electron microscopy and atomic force microscopy. Our findings thus revealed that the orientation of lignin at the surface resulted in increased hydrophobicity of the surface and contributed to the enhancement of water repellency. (C) 2015 Published by Elsevier B.V.

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  • Characterization of the Residue and Liquid Products Produced from Husks of Nuts from Carya cathayensis Sarg by Hydrothermal Carbonization

    Wei Yang, Toshinori Shimanouchi, Yukitaka Kimura

    ACS SUSTAINABLE CHEMISTRY & ENGINEERING   3 ( 4 )   591 - 598   2015.4

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    Hydrothermal carbonization is considered to be an environment-friendly method to pretreat biomass. Therefore, hydrothermal carbonization of the husks of nuts from Carya cathayensis Sarg,, at various temperatures and pHs were performed and the fuel properties of the obtained residue, water-soluble products (WSP) and acetone-soluble products (ASP) were characterized. It was found that the residue yield decreased with increasing hydrothermal carbonization temperature while the yield of total soluble products (WSP and ASP) was nearly the same. The residue and total soluble products yields had no large changes in the pH range of 4.0-10.0, but fewer residues and more total soluble products were obtained at pH 13.0. It was confirmed that hemicellulose was more reactive than cellulose and lignin during hydrothermal carbonization and that lignin could be effectively hydrolyzed/decomposed at pH 13.0. The higher heating values (HHVs) of residues were in the ranges of 22.0 to 28.2 MJ kg(-1) at temperatures from 180 to 260 inverted perpendicular C and pH from 4.0 to 10.0. The HHVs of these residues could be comparable with those of some commercial coals, such as lignite. However, the HI-IVs of residues decreased when the pH reached to 13.0. The WSP and ASP showed lower HHVs compared with heavy fuel oils, which meant the HHVs of WSP and ASP should be increased before being applied as bio-fuels.

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  • Elevating the fuel properties of Humulus lupulus, Plumeria alba and Calophyllum inophyllum L. through wet torrefaction

    Wei Yang, Toshinori Shimanouchi, Miki Iwamura, Yuki Takahashi, Ryota Mano, Kohei Takashima, Tatsuya Tanifuji, Yukitaka Kimura

    FUEL   146   88 - 94   2015.4

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    Wet torrefaction is an effective process to improve the fuel properties of a biomass. However, different biomasses have different component weight ratios and it is possible that not all biomasses are suitable for wet torrefaction treatment. Here we conducted wet torrefaction using three types of biomass with different component weight ratios: Humulus lupulus (HL, the common hop), Plumeria alba (PA, an evergreen shrub) and Calophyllum inophyllum L. (CIL, an evergreen tree). The fuel properties of the obtained solid fuels were characterized. We found that lignin made the main contribution to the solid fuel yield. The reactivity of cellulose and hemicellulose in each biomass was affected by the biomass species and the component weight ratio of the biomass. The wet torrefaction was observed to efficiently elevate the fuel properties of carbon content, atomic H/C and O/C ratios, higher heating value (HHV) and hydrophobicity of all three types of biomass. The HHVs of the solid fuels prepared at 260 degrees C are comparable to those of commercial coals such as Northumerland No. 81/2 Sem. Anth. Coal, Jhanjra Bonbahal Seam Coal-R-VII, and German Braunkohole lignite. These solid fuels could be co-combusted with German Braunkohole lignite without a significant change in the combustion properties of German Braunkohole lignite because of their similar atomic H/C and O/C ratios as well as HHVs. At the wet torrefaction temperature of 260 degrees C, the solid fuel delivered from CIL had lower HHV compared to those from HL and PA although CIL contained the highest lignin content, which has a higher HHV than those of cellulose and hemicellulose. Scanning electron microscopy images of the solid fuels revealed that wet torrefaction was able to completely destroy the biomass surface and create numerous pores and cracks on the solid fuels surface, indicating that the solid fuels have the potential to be used as a source of carbon materials such as activated carbon in addition to their use as bio-fuels. (C) 2015 Elsevier Ltd. All rights reserved.

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  • ToF-SIMS observation for evaluating the interaction between amyloid β and lipid membranes. International journal

    Satoka Aoyagi, Toshinori Shimanouchi, Tomoko Kawashima, Hideo Iwai

    Analytical and bioanalytical chemistry   407 ( 10 )   2859 - 63   2015.4

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    The adsorption behaviour of amyloid beta (Aβ), thought to be a key peptide for understanding Alzheimer's disease, was investigated by means of time-of-flight secondary ion mass spectrometry (ToF-SIMS). Aβ aggregates depending on the lipid membrane condition though it has not been fully understood yet. In this study, Aβ samples on different lipid membranes, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), were observed with ToF-SIMS and the complex ToF-SIMS data of the Aβ samples was interpreted using data analysis techniques such as principal component analysis (PCA), gentle-SIMS (G-SIMS) and g-ogram. DOPC and DMPC are liquid crystal at room temperature, while DPPC is gel at room temperature. As primary ion beams, Bi3(+) and Ar cluster ion beams were used and the effect of an Ar cluster ion for evaluating biomolecules was also studied. The secondary ion images of the peptide fragment ions indicated by G-SIMS and g-ogram were consistent with the PCA results. It is suggested that Aβ is adsorbed homogeneously on the liquid-crystalline-phase lipid membranes, while it aggregates along the lipid on the gel-phase lipid membrane. Moreover, in the results using the Ar cluster, the influence of contamination was reduced.

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  • Characterization of hydrochar prepared from hydrothermal carbonization of peels of Carya cathayensis sarg

    Wei Yang, Toshinori Shimanouchi, Yukitaka Kimura

    DESALINATION AND WATER TREATMENT   53 ( 10 )   2831 - 2838   2015.3

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    Hydrothermal carbonization of peels of Carya cathayensis sarg (PCCS) by subcritical water or acetone- and ethanol-modified subcritical water was carried out at the temperature from 280 to 360 degrees C and coal-like hydrochar was obtained. The hydrochar yield decreased with increasing of treatment temperature, but the higher heating value (HHV) and carbon content of hydrochar were promoted at higher treatment temperature. The HHV of hydrochar was in range of 30-46 MJ/kg with an increase from 52.4 to 127.6%, compared to the original PCCS. The HHVs of hydrochars obtained at 360 degrees C could be comparable with those of heavy fuel oil (42.9 MJ/kg) and diesel oil (45.7 MJ/kg). The O/C and H/C values of hydrochar were similar to those of lignite and subbituminous, except the ash content. Especially, the O/C and H/C values of hydrochars prepared at 360 degrees C could be compatible with those of bituminous. When treated with acetone- or ethanol-modified subcritical water, a synergistic effect of acetone-water or ethanol-water for hydrothermal carbonization was observed; the liquefaction rate with acetone-water or ethanol-water mixture was larger than that of water or acetone or ethanol only. There was no significant change on the O/C and H/C values and HHV on the hydrochar, although more PCCS, was decomposed when using acetone-water or ethanol-water mixture.

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  • Discrimination of Target Proteins Using Arrayed Fluorescent Liposomes Incorporated with Cholesterol by Principal Component Analysis

    R. Imamura, Z. Zhang, T. Yoshikawa, N. Murata, K. Yamashita, M. Fukuzawa, M. Noda, T. Shimanouchi

    2015 IEEE SENSORS   1231 - 1234   2015

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    A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were discriminated with high cumulative contribution ratio.

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  • Target Protein Discrimination Based on Array Sensor Using Calcein-Encapsulating Liposomes with Cholesterol by Principal Component Analysis

    R. Imamura, Z. Zhang, T. Yoshikawa, T. Shimanouchi, N. Murata, K. Yamashita, M. Fukuzawa, M. Noda

    EUROSENSORS 2015   120   699 - 702   2015

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    A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were clearly discriminated with high cumulative contribution ratio. (C) 2015 Published by Elsevier Ltd.

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  • Systematical characterization of phase behaviors and membrane properties of fatty acid/didecyldimethylammonium bromide vesicles. International journal

    Keishi Suga, Tomoya Yokoi, Dai Kondo, Keita Hayashi, Seiichi Morita, Yukihiro Okamoto, Toshinori Shimanouchi, Hiroshi Umakoshi

    Langmuir : the ACS journal of surfaces and colloids   30 ( 43 )   12721 - 8   2014.11

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    Fatty acids (FAs) are known to form vesicle structures, depending on the surrounding pH conditions. In this study, we prepared vesicles by mixing FAs and a cationic surfactant, and then investigated their physicochemical properties using fluorescence spectroscopy and dielectric dispersion analysis (DDA). The assemblies formed from oleic acid (OA) and linoleic acid (LA) were modified by adding didecyldimethylammonium bromide (DDAB). The phase state of FA/DDAB mixtures was investigated with pH titration curves and turbidity measurements. The trigonal diagram of FA/ionized FA/DDAB was successfully drawn to understand the phase behaviors of FA/DDAB systems. The analysis of fluidities in the interior of the membrane with use of 1,6-diphenyl-1,3,5-hexatriene (DPH) indicated that the membrane fluidities of OA/DDAB and LA/DDAB at pH 8.5 slightly decreased in proportion to the molar ratio of DDAB in FA/DDAB systems. The fluorescent probe 6-lauroyl-2-dimethylamino naphthalene (Laurdan) indicated that the LA vesicle possessed a dehydrated surface, while the OA vesicle surface was hydrated. Modification of LA vesicles with DDAB induced the hydration of membrane surfaces, whereas modification of OA vesicles by DDAB had the opposite effect. DDA analysis indicated that the membrane surfaces were hydrated in the presence of DDAB, suggesting that the surface properties of FA vesicles are tunable by DDAB modification.

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  • Investigation of the Degradation Kinetic Parameters and Structure Changes of Microcrystalline Cellulose in Subcritical Water

    Wei Yang, Toshinori Shimanouchi, Shengji Wu, Yukitaka Kimura

    ENERGY & FUELS   28 ( 11 )   6974 - 6980   2014.11

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    Cellulose is the most abundant nature polymer and the main component in all biomass. In this study, the degradation kinetic parameters and structure changes of microcrystalline cellulose (MCC), which served as fundamental studies for degradation of biomass in subcritical water, were investigated at the temperature range from 100 to 300 degrees C. The yield of the MCC residue began to decrease at 205 degrees C and reached the lowest value at 275 degrees C. However, it howed an increase at the temperature higher than 275 degrees C. The degradation area of MCC (205275 degrees C) was separated into zones 1 and 2 with 245 degrees C as a boundary. The activation energy (E), pre-exponential factor (A), and reaction order (n) of MCC in each zone were 226.5 kJ mol(-1), 2.3 x 10(23) s(-1), and 0.6 (zone 1) and 423.1 kJ mol(-1), 9.0 x 10(40) s(-1), and 0.5 (zone 2), respectively. There showed a breaking point of 245 degrees C for the Arrhenius plot in the reaction area. The surface morphology of the MCC residue had no significant change below 260 degrees C, as indicated by scanning electron microscopy (SEM) images. However, it was completely destroyed at the temperature above 275 degrees C, and MCC residues with strong pore structures were obtained at higher temperatures. The structures of the crystalline region of the MCC residue below 275 degrees C had no significant change, although 89.2% MCC was degraded at 275 degrees C. However, they nearly disappeared at higher temperatures with the steeply reducing crystallinity index of MCC at the same time. As for the degradation mechanism of MCC, it was proposed that only the surface of MCC was degraded at 205-245 degrees C and the hydrogen and glycosidic bonds on the interior of MCC fibrils were destroyed at the temperature range from 245 to 275 degrees C. Finally, the remaining MCC as well as generated oligomer and monomer were further degraded at higher temperatures.

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  • Relationship between the mobility of phosphocholine headgroup and the protein-liposome interaction: a dielectric spectroscopic study. International journal

    Toshinori Shimanouchi, Noriko Yoshimoto, Azusa Hiroiwa, Keiichi Nishiyama, Keita Hayashi, Hiroshi Umakoshi

    Colloids and surfaces. B, Biointerfaces   116   343 - 50   2014.4

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    Proteins could affect the headgroup mobility of phospholipid within liposome membranes through the protein-liposome interaction. The variation of headgroup mobility of phospholipid was then investigated by using the dielectric dispersion analysis. The eight proteins (Mw = 4.2-28.7 kDa) were used to investigate the protein-liposome interaction. It has been revealed that the strength of the protein-liposome interaction at 25 °C was linearly correlated with the stability of intramolecular hydrogen bondings of proteins, better than with their hydrophobicity and the surface charge density. Overall, liposomes composed of binary lipid system, appeared to strongly interact with proteins, in contrast to liposomes composed of single, ternary, and quaternary lipid systems. This is probably because liposomes composed of binary lipid system favored to form the microscopic environment where proteins could interact. The present result suggested the heterogeneous phase state of lipid membranes was one of dominant factors for the interaction between proteins and lipid membranes.

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  • Rapid Conversion of Glycerol to Lactic Acid under Alkaline Hydrothermal Conditions, by Using a Continuous Flow Reaction System

    Toshinori Shimanouchi, Shouhei Ueno, Kazuki Shidahara, Yukitaka Kimura

    CHEMISTRY LETTERS   43 ( 4 )   535 - 537   2014.4

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    A rapid conversion of glycerol to lactic acid (lactate) could be successfully achieved under alkaline hydrothermal conditions, by using a continuous flow reaction system. A rapid conversion by a continuous flow-type reaction system made it possible to achieve the reaction under high-temperature conditions without side reactions. The rapid conversion and high reaction yield (reaction: 2 min; yield: 90%) resulted from the rapid temperature-shift advantageous for the inhibition of side reactions.

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  • Extraction of reducing sugar with anti-oxidative scavengers from peels of Carya cathayensis sarg.: Use of subcritical water

    Toshinori Shimanouchi, Shohei Ueno, Wei Yang, Yukitaka Kimura

    Environmental Engineering Research   19 ( 1 )   41 - 45   2014.3

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    The peels of Carya cathayensis Sarg. (PCCS) were treated under subcritical water conditions (130°C to 280 °C for 0 to 120 min). The extract from PCCS included reducing sugar, proteins, and compounds with radical scavenging activity. Addressing the reducing sugar that is a resource of bioethanol, we could maximize the reducing sugar under the subcritical water (190°C for 60 min) and obtain 0.24 g/g-sample together with 9.7 units/mg-sample of radical scavenging activity. The obtained extract was estimated to correspond to 1 L of bioethanol/100 g-sample. It was therefore considered that the treatment by subcritical water could yield reducing sugar and natural compounds with radical scavenging activity. © 2014 Korean Society of Environmental Engineers.

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  • Development of metal affinity-immobilized liposome chromatography and its basic characteristics

    Hideto Nagami, Hiroshi Umakoshi, Takenori Kitaura, Gary Lee Thompson, Toshinori Shimanouchi, Ryoichi Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL   84   66 - 73   2014.3

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    Metal affinity-immobilized liposome chromatography (MA-ILC) was newly developed as a chromatographic technique to separate and analyze peptides. The MA-ILC matrix gel was first prepared by immobilizing liposomes modified with functional ligands. The functional ligand used to adsorb metal ions was N-hexadecyl iminodiacetic acid (HIDA), which is obtained by attaching a long alkyl chain to an iminodiacetic acid (IDA). Cu(II) ion was first adsorbed on the gel matrix through its complex formation with the HIDA on the surface of the immobilized liposome. Synthetic peptides of various types ranging in size from 5 to 40 residues were then used, and their retention properties on the MA-ILC were evaluated. The retention property of peptides on the MA-ILC by using a usual imidazole elution was compared with the retention property in the case of the immobilized metal affinity chromatography (IMAC) and an immobilized liposome chromatography (ILC). It was found that the retention property of peptides on the MA-ILC has the features of both the IMAC and the ILC; the retention ability of peptides depends on both the number of histidine residues in peptides and the liposome membrane affinity of the peptides. Histidine and tryptophan residues among amino acid residues in peptides indicated a high contribution coefficient for the peptide retention on the MA-ILC, probably due to their metal ion and membrane interaction properties, respectively. (C) 2014 Elsevier B.V. All rights reserved.

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  • Subcritical Water-Assisted Emulsification of Decane/Water: Influence of Surfactants

    Toshinori Shimanouchi, Takashi Tange, Yukitaka Kimura

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   21   103 - 110   2014

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    A finely monodispersed oil-in-water (O/W) emulsion was obtained under high temperature and pressure conditions (240 degrees C and 10 MPa). A coarse emulsion stabilized by Tween 20 was prepared in advance from the decane / water system to be treated under the subcritical conditions. The treated coarse emulsion was thereafter mixed with the aqueous solution including surfactants to improve the monodispersity of the oil droplets. The subcritical condition could supply sufficient energy to produce finely dispersed oil droplets (similar to 233 nm in diameter).

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  • Comparison of the Interfacial Properties of Span 80 Vesicle, W/O Emulsions and Liposomes

    Keita Hayash, Hiroshi Umakosile, Toshinori Shimanouchi

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   21   191 - 199   2014

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    Vesicles composed of sorbitan monooleate (Span 80) were prepared by a two-step emulsification method. The water-in-oil (W/O) emulsions generated in the first step of emulsification exhibited a quite dynamic and large hydrophobic difference between water and hexane. A subsequent emulsification yielded the vesicles with a fluid and flexible interface, as compared with that of liposomes. It was furthermore demonstrated that the two-step emulsification method has the advantage of the introduction of a PEG layer into the outer surface of the vesicles, while retaining the membrane properties of the vesicles.

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  • Subcritical Water-Assisted Emulsification of Oil/Water: The Effect of the Solubility of the Organic Solvent and Surfactants

    Toshinori Shimanouchi, Takashi Tange, Yukitaka Kimura

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   21   223 - 230   2014

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    The formation of finely monodispersed oil-in-water (O/W) emulsions under high temperature and pressure conditions (240 degrees C and 10 MPa) depended on the solubility of the oil in water. Fatty acids with large solubilities in water favored the formation of oil droplets in emulsions of less than 100 nm in diameter. The water dissolved in the oil droplets participated throughout the cooling process, followed by the break-down of the oil droplets. In contrast, organic solvents with lower solubilities in water formed oil droplets in emulsions that had at most a diameter of 300 nm.

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  • Water/Methyl isobutyl ketone (MIBK) Biphasic System in Slug Flow under High Temperature and Pressure Conditions

    Toshinori Shimanouchi, Tatsuya Tanifuji, Satoko Fujioka, Koichi Terasaka, Yukitaka Kimurai

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   21   201 - 209   2014

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    The clarification of the partition property of substrates in a water / organic biphasic system is useful in the development of a reaction / separation process for value-added materials, especially under high temperature and pressure conditions. In this study, the water / methyl isobuthyl ketone (MIBK) biphasic system was investigated under various temperatures (25 190 degrees C) at 10 MPa. The partitioning behavior of substrates such as furfural derivatives, amino acids, and saccharides, depended on the temperature and the flow rate ratio of MIBK to the water phase. The scale of hydrophobicity (HF) of the water / MIBK biphasic system to determine the partitioning behavior of substrates was estimated based on the partitioning behavior of amino acids. The HF value for the water / MIBK phase flow system of was greater than that for the batch system and comparable to that for conventional aqueous two-phase systems such as polyethylene glycol / dextran and liposome membrane systems. It was probably because an increase of the surface-to-volume ratio and the vortex field in the slug flow contributed to the mass transfer of substrates and their distribution.

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  • Membrane interaction between Span 80 vesicle and phospholipid vesicle (liposome): Span 80 vesicle can perturb and hemifuse with liposomal membrane. International journal

    Keita Hayashi, Tsuyoshi Tatsui, Toshinori Shimanouchi, Hiroshi Umakoshi

    Colloids and surfaces. B, Biointerfaces   106   258 - 64   2013.6

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    We have focused on the interaction between the Span 80 vesicle and phospholipid vesicle (liposome). The Span 80 vesicle was mixed with a phospholipid vesicle (liposome), used as a simplified model of plasma membrane. From calcein leakage experiments, it was revealed that the interaction between the Span 80 vesicles and the liposomes could perturb the liposome membrane. In the experiments based on fluorescence resonance energy transfer, the lipid mixing between Span 80 vesicle and liposome was observed. The above phenomena were also supported by the fluorescence spectroscopic analysis using laurdan, resulting in the variation in the membrane properties of liposome after its mixing with Span 80 vesicle. These results suggest that the Span 80 vesicles can easily interact and hemifuse with the liposome membrane, which depend on the membrane properties of the Span 80 vesicle, "flexible" and "molecular structure" of the components.

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  • Membrane fusion mediated by phospholipase C under endosomal pH conditions

    Toshinori Shimanouchi, Hidenori Kawasaki, Makoto Fuse, Hiroshi Umakoshi, Ryoichi Kuboi

    Colloids and Surfaces B: Biointerfaces   103   75 - 83   2013.3

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    Phospholipase C (PLC) is considered to be one of key enzymes for the design of drug delivery system using the endocytosis route, because PLC can catalyze the membrane fusion between cell membranes and phospholipid vehicles (liposomes). Membrane fusion by PLC was then studied under various pHs to model the endosomal environment. The used liposomes were composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and cholesterol (Ch). The membrane fusion was dominated by the enzymatic reaction at pH 6-7.5. In contrast, the membrane perturbation effect due to the conformational change of PLC could induce the membrane fusion at around pH 4. The maximal value of membrane fusion was observed at around pH 5 for three liposomes in the order of DOPC &lt
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    DOPC/DPPC/Ch (1:1:1). From the experiments with the hydrophobic fluorescence probe and the circular dichroic method, it was revealed that PLC took a molten-globule state, with a large fluctuation and an enzymatic activity, at around pH 4-5. The DAG-rich domain enzymatically produced by PLC played role for the field for the membrane perturbation enough to lead to the membrane fusion. The present finding would be helpful to understand the behavior of membrane fusion under the late endosomal pH condition in cell system. © 2012 Elsevier B.V.

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  • Growth behavior of giant vesicles using the electroformation method: Effect of proteins on swelling and deformation

    Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

    JOURNAL OF COLLOID AND INTERFACE SCIENCE   394   269 - 276   2013.3

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    The growth of giant vesicles (GVs) can be considered as a consecutive process of swelling/detachment/deformation, which is a response of lipid membranes on solid surfaces to the solvent and environmental factors such as temperature and ionic strength. The electroformation method allows to visualize the responses to such factors. The additive effect of the protein on the growth of GVs, composed of zwitterionic phospholipids, was herein investigated using the electroformation method. Proteins denatured by a pH-shift (to be in the Molten Globule state) perturbed the lipid membranes, resulting in the acceleration of GV growth. The GVs detached from the electrode showed deformation close to a stomatocyte. It was revealed that common factor for the response of lipid membranes was the variation of the apparent area elastic modulus associated with the interaction between proteins and lipid membranes. The present finding affords better understanding about the response of lipid membranes on solid surfaces under a variety of environment factors. (C) 2012 Elsevier Inc. All rights reserved.

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  • Growth behavior of A beta protofibrils on liposome membranes and their membrane perturbation effect

    Toshinori Shimanouchi, Keiichi Nishiyama, Azusa Hiroiwa, Huong Thi Vu, Nachi Kitaura, Hiroshi Umakoshi, Ryoichi Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL   71   81 - 88   2013.2

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    The protofibrils of amyloid fibrils have recently drawn an attention due to their cytotoxicity although the physicochemical properties of the protofibrils as an intermediate of fibrillation process have still remained unclear. We investigated the growth behavior of the protofibrils of amyloid beta protein with 40 amino acid residues in the presence of various liposornes and the membrane perturbation (calcein leakage). The growth behavior of protofibrils was apparently correlated with the protofibrils-induced calcein leakage. From the binding experiment using a hydrophobic fluorescence probe and the quartz crystal microbalance method combined with the planar lipid membrane, it is suggested that the apparent correlation between the growth behavior of protofibrils and their membrane perturbation resulted from the physicochemical properties of protofibrils such as hydrophobicity and the stability of hydrogen bonds. The protofibrils were found to show the highest hydrophobicity and the highest instability of hydrogen bonds of the proteins used in this study (monomer, protofibrils, matured fibrils, and other three typical proteins). It is anticipated that such properties of protofibrils were advantageous both for the binding of protofibrils with monomer (growth behavior) and for the binding of lipid bilayer membranes (membrane perturbation). (C) 2012 Elsevier B.V. All rights reserved.

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  • Effect of liposome membranes on disaggregation of amyloid beta fibrils by dopamine

    Huong Thi Vu, Toshinori Shimanouchi, Daisuke Ishikawa, Tadaharu Matsumoto, Hisashi Yagi, Yuji Goto, Hiroshi Umakoshi, Ryoichi Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL   71   118 - 126   2013.2

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    The inhibition of fibril formation of amyloid beta (A beta) and the disaggregation of A beta fibrils are the promising approaches for a medical treatment of Alzheimer's disease (AD) therapy. In this study, we investigated the effects of liposomes on dopamine-induced disaggregation of A beta fibrils by using the variety of liposomes. The used liposomes were normal liposomes, raft-forming liposomes, charged liposomes and oxidized liposomes. Those liposome could accelerate the disaggregation rate of fibrils. From the comparison of normal and charged liposomes, a certain contribution of dopamine via an electrostatic interaction to the disaggregation was confirmed. From raft-forming and oxidized liposomes, we revealed a significant contribution of bound water to liposomes, which could assist the formation of the quinine-form of dopamine by a removal of its proton. It is, therefore, concluded that the membrane surface of liposomes is considered to be an adequate environment for the dopamine-induced disaggregation of fibrils. (c) 2012 Elsevier B.V. All rights reserved.

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  • A new discrimination method of target biomolecules with miniaturized sensor array utilizing liposome encapsulating fluorescent molecules with time course analysis

    K. Takada, T. Fujimoto, T. Shimanouchi, M. Fukuzawa, K. Yamashita, H. Umakoshi, M. Noda

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   1   194 - 196   2013.1

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    This paper reports a new type of detection approach by arrayed biosensor for discrimination of target biomolecules such as protein and/or evaluation of their concentrations, utilizing liposome encapsulating fluorescent molecules and the corresponding time course analysis of the fluorescence. This method is realized by obtaining multi-dimensional data plots (curve) of fluorescent output such as its intensity from plural and different liposomes independent from each other. Two different proteins (carbonic anhydrase from bovine: CAB and Lysozyme), including their concentrations, were clearly discriminated by the developed method. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.

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  • Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) Vesicles

    Keita Hayashi, Tsuyoshi Tatsui, Toshinori Shimanouchi, Hiroshi Umakoshi

    INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES   9 ( 2 )   142 - 148   2013

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    Span 80 (sorbitan monooleate) vesicles behaved differently from conventional phospholipid vesicles (liposomes) because the former had a more fluid interface. After doxorubicin hydrochloride (DOX) was encapsulated into the Span 80 vesicle (loading efficiency: 63 %), DOX-loaded Span 80 vesicles (DVs) were thereafter added to Colon 26 cells. It was suggested, from the flow cytometric analysis and confocal laser microscopic observation, that DVs directly deliver DOX into the cytoplasm of Colon 26 cells. DVs showed the different delivery manner from the DOX-loaded liposomes (DLs). It is considered that the difference of delivery manner between DVs and DLs resulted in the difference of cytotoxicity (IC50); i.e. IC50 values for DVs and DLs were 5 and > 30 mu M, respectively. The results obtained herein would give the fundamental findings which can contribute to the improvement of formulation of conventional liposome-based carrier and its cytotoxicity.

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  • Simplified Model for Extraction of 5-Hydroxymethylfurfural from Fructose: Use of Water/Oil Biphasic System under High Temperature and Pressure Conditions

    Toshinori Shimanouchi, Yoshitaka Kataoka, Masahiro Yasukawa, Tsutomu No, Yukitaka Kimura

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   20   205 - 212   2013

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    5-Hydroxymethylfurfural (HMF) was obtained from the dehydration of fructose under high temperature and pressure conditions (180 degrees C and 10 MPa). To avoid the decomposition of HMF in the bulk aqueous phase, we dehydrated fructose in the water / metyl isobuthyl ketone (MIBK) biphasic system. The HMF could be successfully extracted into the organic MIBK phase without further decomposition of HMF. We present a simplified model taking into consideration of the slug flow with the wall film to maximize the HMF yield in the MIBK phase. It was likely that the slug flow contributed to an increase in the surface-to-volume ratio for the mass transfer of HMF.

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  • Secondary nucleation of A beta fibrils on liposome membrane

    Toshinori Shimanouchi, Nachi Kitaura, Ryo Onishi, Hiroshi Umakoshi, Ryoichi Kuboi

    AICHE JOURNAL   58 ( 12 )   3625 - 3632   2012.12

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    The amyloid fibrils of amyloid beta protein (A beta) from Alzheimer's disease are likely to show the cytotoxicity, depending on their morphology. The relationship between the nucleation kinetics of the A beta fibrils and their morphology has been investigated. From the perspective of a crystallization technique assuming primary/secondary nucleation steps and an elongation step, the secondary nucleation rate B [# m-3 s-1], was experimentally and coarsely determined by using total internal reflection fluorescence microscopy combined with thioflavin T. In an aqueous solution, linear and rigid fibrils were formed with a relatively smaller B value ((2.83 +/- 0.55) x 105 # m-3 s-1), whereas spherulitic amyloid assemblies were formed in the presence of negatively charged liposome including oxidized lipids, with a larger B value ((7.65 +/- 0.47) x 105 # m-3 s-1). Those findings should lead to a better understanding of the mechanism for the formation of fibrils and senile plaques in Alzheimer's disease. (C) 2012 American Institute of Chemical Engineers AIChE J, 2012

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  • Formation of spherulitic amyloid beta aggregate by anionic liposomes

    Toshinori Shimanouchi, Naoya Shimauchi, Ryo Ohnishi, Nachi Kitaura, Hisashi Yagi, Yuji Goto, Hiroshi Umakoshi, Ryoichi Kuboi

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   426 ( 2 )   165 - 171   2012.9

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    Alzheimer's disease is the most common form of senile dementia. This neurodegenerative disorder is characterized by an amyloid deposition in senile plaques, composed primarily of fibrils of an aggregated peptide, amyloid beta (A beta). The modeling of a senile plaque formation on a model neuronal membrane under the physiological condition is an attractive issue. In this study, we used anionic liposomes to model the senile plaque formation by A beta. The growth behavior of amyloid All fibrils was directly observed, revealing that the induction of the spherulitic A beta aggregates could result from the growth of seeds in the presence of anionic liposomes. The seeds of A beta fibrils strongly interacted with negatively charged liposome and the subsequent association of the seeds were induced to form the seed cluster with many growth ends, which is advantageous for the formation of spherulitic A beta aggregates. Therefore, anionic liposomes mediated not only fibril growth but also the aggregation process. These results imply that anionic liposome membranes would affect the aggregate form of All fibrils. The modeling of senile plaque reported here is considered to have great potential for study on the amyloidosis. (c) 2012 Elsevier Inc. All rights reserved.

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  • Membranomics Research Using Liposome Immobilization Technique

    SHIMANOUCHI Toshinori

    Seibutsu Butsuri   52 ( 3 )   154 - 155   2012.5

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  • Effect of copper (II) ion against elongation behavior of amyloid ss fibrils on liposome membranes

    T. Shimanouchi, R. Onishi, N. Kitaura, H. Umakoshi, R. Kuboi

    CRYSTAL RESEARCH AND TECHNOLOGY   47 ( 1 )   101 - 108   2012.1

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    The fibril growth behavior of amyloid beta protein (A beta) on cell membranes is relating to the progression of Alzheimer's disease. This growth behavior of A beta fibrils is sensitively affected by the metal ions, neurotransmitters, or bioreactive substrate. The inhibitory effect of those materials was quantitatively estimated from the viewpoints of crystal growth. In a bulk aqueous solution, copper (II) ion showed the strong inhibitory effect on the growth of A beta fibrils. Meanwhile, the addition of a closed-phospholipid bilayer membrane (liposome) could reduce the above inhibitory effect of copper (II) ion. (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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  • A New Biosensing by Dielectric Dispersion Analysis of Interaction between Lipid Membrane of Liposome and Target Biomolecules up to 20 GHz Range

    Keisuke Takada, Kaoru Yamashita, Minoru Noda, Toshinori Shimanouchi, Hiroshi Umakoshi

    2012 IEEE SENSORS PROCEEDINGS   935 - 938   2012

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    This paper reports, firstly, that it was newly found by our specific Dielectric Dispersion Analysis (DDA) that plural type of bound water with different relaxation frequencies exist in a suspension of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Secondly, we observe that the interaction between the liposome and a specific target biomolecule depends on the type of target molecules (we used a protein of carbonic anhydrase from bovine (CAB) and/or cholesterol, respectively), leading to the change in dielectric relaxation at different relaxation frequency of bound water. It is finally concluded that the developed DDA method allows us to acquire the quantitative information with respect to bound water to liposomes, which gives novel physical insight on interaction between liposomes and target biomolecules (especially, protein).

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  • A planar-type leakage current and impedance microsensor for detection of interaction between electrolyte-entrapping liposome and protein

    Y. Ohara, P. Lorchirachoonkul, K. Yamashita, M. Noda, O. A. Alberto, T. Shimanouchi

    IMFEDK 2012 - 2012 International Meeting for Future of Electron Devices, Kansai   146 - 147   2012

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    We have developed a new leakage current microsensor by using simpler planar processes than Si-surface-bulk micromachining processes used in the previous microwell structure. This sensor fabrication and structure can easily make a target solution volume smaller than μL with excellent immobilization of the droplet and intact biomolecules as sensing elements, as a result, reduce effectively the background noise current in the microsensor and improve reproducibility of the results. The leakage current due to the biochemical interaction was successfully evaluated. Cole-Cole plots from the impedance analysis also show quantitative difference between with and without the interaction, depending on the charge-transfer impedance. © 2012 IEEE.

    DOI: 10.1109/IMFEDK.2012.6218624

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  • A New Intact Immobilization of Liposome as Sensing Bio Nano-Particle on Oxidized Metal Electrode Surface

    Minoru Noda, Keisuke Takada, Mariko Nakai, Kaoru Yamashita, Toshinori Shimanouchi, Hiroshi Umakoshi

    2012 IEEE SENSORS PROCEEDINGS   25 - 28   2012

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    We newly report that the intactness of liposome on the electrode surfaces of Pt and Au metals could be significantly improved on thermally oxidized metal surfaces, compared to those with non-oxidization or even with a SAM treatment. It is especially noted that the sub-gel phase of lipid membrane of the liposome on the oxidized Pt surface is much more suppressed than the case of non-oxidized, indicating that the fluidity of intact liposome greatly improves, as the sub-gel phase then crystalline phase will increase when the intact liposome loses its closed spherical shape. Finally, the retention time of the intact liposome on the oxidized surface becomes improved to about one day. These results indicate that the oxidation treatment made the metal surface hydrophilic enough to keep the stable and intact liposome. We think that the retention time up to one day is good enough for practical biosensor applications using intact liposome to improve device performance and its reliabilities.

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  • Copper-mediated growth of amyloid beta fibrils in the presence of oxidized and negatively charged liposomes

    Toshinori Shimanouchi, Ryo Onishi, Nachi Kitaura, Hiroshi Umakoshi, Ryoichi Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   112 ( 6 )   611 - 615   2011.12

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    Amyloid beta protein (A beta) from Alzheimer's disease formed fibrillar aggregates and their morphology depended on oxidized and negatively charged liposomes. The morphology of fibrillar aggregates was affected by Cu2+, together with their growth kinetics. This is because Cu2+ inhibited the nucleation step in the formation of amyloid A beta fibrillar aggregates by forming A beta/Cu complex inactive to the growth of fibrillar aggregates. In addition, this is probably because Cu2+ affected the fibrillar aggregate formed on the surface of liposomes. These findings would give a better understanding of the formation mechanism of amyloid fibrils on biomembranes. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.

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  • Relationship between the mobility of phosphocholine headgroups of liposomes and the hydrophobicity at the membrane interface: A characterization with spectrophotometric measurements

    Toshinori Shimanouchi, Masashi Sasaki, Azusa Hiroiwa, Noriko Yoshimoto, Kazuya Miyagawa, Hiroshi Umakoshi, Ryoichi Kuboi

    COLLOIDS AND SURFACES B-BIOINTERFACES   88 ( 1 )   221 - 230   2011.11

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    In this study, we investigated the dynamics of a membrane interface of liposomes prepared by eight zwitterionic phosphatidylcholines in terms of their headgroup mobility, with spectroscopic methods such as dielectric dispersion analysis (DDA), fluorescence spectroscopy. The DDA measurement is based on the response of the permanent dipole moment to a driving electric field and could give the information on the axial rotational Brownian motion of a headgroup with the permanent dipole moment. This motion depended on kinds of phospholipids, the diameter of the liposomes, and the temperature. The activation energy required to overcome the intermolecular force between headgroups of phospholipids depended on the strength of the interaction between headgroups such as hydrogen bonds and/or dipole-dipole interaction. Hydration at the phosphorous group of phospholipid and the molecular order of lipid membrane impaired the interaction between headgroups. Furthermore, the hydrophobicity of membrane surface increased parallel to the increase in headgroup mobility. It is, therefore, concluded that hydration of headgroup promoted its mobility to make the membrane surface hydrophobic. The lipid membrane in liquid crystalline phase or the lipid membrane with the larger curvature was more hydrophobic. (C) 2011 Elsevier B.V. All rights reserved.

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  • Conformational change of single-stranded RNAs induced by liposome binding

    Keishi Suga, Tomoyuki Tanabe, Hibiki Tomita, Toshinori Shimanouchi, Hiroshi Umakoshi

    NUCLEIC ACIDS RESEARCH   39 ( 20 )   8891 - 8900   2011.11

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    The interaction between single-stranded RNAs and liposomes was studied using UV, Fourier Transform Infrared spectroscopy (FTIR) and Circular Dichroism spectroscopy (CD). The effect of the surface characteristics of liposomes, which were composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and modified with cholesterol (Ch) or 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), on the liposome-RNA interaction was investigated. The fluorescence of 6-(p-toluidino)naphthalene-2-sulfonate (TNS) embedded in the liposome surface (epsilon = 30-40) was decreased in the presence of tRNA, suggesting that single-stranded tRNA could bind onto the liposome. The dehydration of -PO(2)(-) -, guanine (G) and cytosine (C) of tRNA molecules in the presence of liposomes suggested both an electrostatic interaction (phosphate backbone of tRNA and trimethylammonium group of POPC, DOTAP) and a hydrophobic interaction (guanine or cytosine of tRNA and aliphatic tail of lipid). The tRNA conformation on the liposome was determined by CD spectroscopy. POPC/Ch (70/30) maintained tRNA conformation without any denaturation, while POPC/DOTAP(70/30) drastically denatured it. The mRNA translation was evaluated in an Escherichia coli cell-free translation system. POPC/Ch(70/30) enhanced expression of green fluorescent protein (GFP) (116%) while POPC/DOTAP(70/30) inhibited (37%), suggesting that the conformation of RNAs was closely related to the translation efficiency. Therefore, single-stranded RNAs could bind to liposomal membranes through electrostatic and hydrophobic attraction, after which conformational changes were induced depending on the liposome characteristics.

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  • Sensitivity Enhancement of Leakage Current Microsensor for Detection of Target Protein by Using Protein Denaturant

    Minoru Noda, Prinya Lorchirachoonkul, Toshinori Shimanouchi, Kaoru Yamashita, Hiroshi Umakoshi, Ryouichi Kuboi

    IEEE SENSORS JOURNAL   11 ( 11 )   2749 - 2755   2011.11

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    We have developed a highly-sensitive leakage current microsensor by using 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) liposome entrapping K-4[Fe(CN)(6)] solution to detect the existence of proteins. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully resulted in a prominent improvement in sensitivity by 129-folds of magnitude. This is because the modulation of conformation of the target proteins, carbonic anhydrase from bovine (CAB) and lysozyme could contribute to the promotion of the interaction between the liposome and the target protein.

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  • Span 80 vesicles have a more fluid, flexible and "wet" surface than phospholipid liposomes

    Keita Hayashi, Toshinori Shimanouchi, Keiichi Kato, Tatsuhiko Miyazaki, Atsushi Nakamura, Hiroshi Umakoshi

    COLLOIDS AND SURFACES B-BIOINTERFACES   87 ( 1 )   28 - 35   2011.10

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    The surface properties of Span 80 vesicles at various cholesterol contents, together with those of various liposomes, were characterized by using fluorescence probes. The membrane fluidity of the Span 80 vesicles was measured by 1,6-dipheny1-1.3.5-hexatriene (DPH) and trimethlyammonium-DPH (TMA-DPH), and the results suggested that the surface of the Span 80 vesicles was fluid due to the lateral diffusion of Span 80 molecules. The depolarization measured by TMA-DPH and the headgroup mobility measured by dielectric dispersion analysis indicated the high mobility of the head group of Span 80 vesicles. This suggested that the surface of Span 80 vesicles was flexible due to the head group structure of Span 80, sorbitol. In addition, spectrophotometric analysis with 6-dodecanoyl-N, N-dimethy1-2-naphthylamine and 8-anilino-1-naphthalenesulfonic acid indicated that the water molecules could easily invade into the interior of the Span 80 vesicle membrane, suggesting that the membrane surface was more wet than the liposome surface. These surface properties indicated that the protein could interact with the interior of vesicle membranes, which was similar to the case of cholesterol. Thus the present results confirmed that the Span 80 vesicle surfaces showed the unique characteristics of fluidity, flexibility, and "wetness", whereas the liposome surfaces did not. (C) 2011 Elsevier B.V. All rights reserved.

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  • Span 80 vesicles have a morefluid, flexibleand"wet"surface than phospholipid liposomes. Reviewed

    Hayashi Keita, Shimanouchi Toshinori, Kato Keiichi, Miyazaki Tatsuhiko, Nakamura Atsushi, Umakoshi Hiroshi

    Colloids and surfaces. B, Biointerfaces   87 ( 1 )   28   2011.10

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    The surface properties of Span 80 vesicles at various cholesterol contents, together with those of various liposomes, were characterized by using fluorescence probes. The membrane fluidity of the Span 80 vesicles was measured by 1,6-diphenyl-1.3.5-hexatriene (DPH) and trimethlyammonium-DPH (TMA-DPH), and theresults suggested that the surface of the Span 80 vesicles was fluid due to the lateral diffusion of Span 80 molecules. The depolarization measured by TMA-DPH and the headgroup mobility measured by dielectric dispersion analysis indicated the high mobility of the head group of Span 80 vesicles. This suggested that the surface of Span 80 vesicles was flexible due to the head group structure of Span 80, sorbitol. In addition, spectrophotometric analysis with 6-dodecanoyl-N, N-dimethyl-2-naphthylamine and 8-anilino-1-naphthalenesulfonic acid indicated that the water molecules could easily invade into the interior of the Span 80 vesicle membrane, suggesting that the membrane surface was more wet than the liposome surface. These surface propertiesindicated that the protein could interact with the interior of vesicle membranes, which was similar to the case of cholesterol. Thus the pr

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  • New Planar-Type Leakage Current and Impedance Microsensor for Detection of Interaction between Electrolyte-Entrapping Liposome and Protein

    P. Lorchirachoonkul, I. Goto, T. Shimanouchi, K. Yamashita, M. Noda

    EUROSENSORS XXV   25   1449 - 1452   2011

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    We have developed a new leakage current microsensor by using simpler planar processes than Si-surface-bulk micromachining processes used in the previous microwell structure. This sensor fabrication and structure can easily make a target solution volume smaller than mu L with excellent immobilization of the droplet and intact biomolecules as sensing elements, as a result, reduce effectively the background noise current in the microsensor and improve reproducibility of the results. The leakage current due to the biochemical interaction was successfully evaluated, dependent on the droplet protein concentration. Cole-Cole plots from the impedance analysis also show quantitative difference between with and without the interaction, depending on the charge-transfer impedance that results from the condition and structure of liposome and lipid membrane after the interaction. (C) 2011 Published by Elsevier Ltd.

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  • Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System

    Hiroshi Umakoshi, Tomoyuki Tanabe, Keishi Suga, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

    INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES   7 ( 3 )   253 - 260   2011

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    Oxidative stress can affect in vitro GFP expression through its control of the gene silencing effect of the liposome prepared by 1,2- dioleoyl-3-trimethyl-ammonium propane ( DOTAP). The gene silencing effect of cationic DOTAP liposome in in vitro GFP expression, especially focusing on its translation process, and the effects of oxidative stress on its silencing effect were investigated. GFP expression, initiated by mRNA, was found to be thoroughly inhibited in the presence of DOTAP liposome at concentration of more than 2.5 mM, though its inhibitory effect was reduced in the presence of hydrogen peroxide. The analyses of (i) the interaction of mRNA with DOTAP, (ii) the chemical structure of DOTAP, and (iii) the membrane fluidity of DOTAP liposome imply the possible role of gene expression by the liposome membrane and stress conditions.

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  • Significant improvement in sensitivity of leakage current microsensor by using denaturant and electrolyte-entrapping DPPC liposomes

    P. Lorchirachoonkul, T. Shimanouchi, K. Yamashita, H. Umakoshi, R. Kuboi, M. Noda

    14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010   3   1982 - 1984   2010.12

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    We have developed a highly-sensitive leakage current microsensor by using DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads to obtain a prominent improvement although target protein, which is carbonic anhydrase from bovine (CAB) and lysozyme, weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB and lysozyme protein such as 'Native', 'Molten-Globule' and 'Unfold' states.

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  • Development of LIPOzymes Based on Biomembrane Process Chemistry

    Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi

    Pharmaceutical Process Chemistry   421 - 441   2010.10

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    DOI: 10.1002/9783527633678.ch21

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  • Cationic liposome can interfere mRNA translation in an E. coli cell-free translation system

    Huong Thi Bui, Hiroshi Umakoshi, Keishi Suga, Tomoyuki Tanabe, Kien Xuan Ngo, Toshinori Shimanouchi, Ryoichi Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL   52 ( 1 )   38 - 43   2010.10

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    The effect of cationic liposome prepared from 1,2-dioleoyl-3-trimethyl- ammonium-propane (DOTAP) on the gene expression at the mRNA translation level was investigated using an E. coli cell-free translation system. DOTAP liposome at 3 mM inhibited the mRNA translation of green fluorescent protein (GFP), as indicated both by the fluorescence intensity of GFP and by SDS-PAGE analysis. The role of DOTAP liposome on the inhibition of mRNA translation was revealed that the cationic quaternary amine groups on the liposome surface can interact and neutralize the anionic phosphate groups on mRNA by an electrostatic interaction. mRNA molecules still existed without any degradation in the presence of DOTAP liposome although it could not be translated. These results clearly illustrated that the DOTAP liposome could knock down mRNA and silence its activity of translation in an E. coli cell-free system. (C) 2010 Elsevier B.V. All rights reserved.

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  • Catechol derivatives inhibit the fibril formation of amyloid-beta peptides

    Vu Thi Huong, Toshinori Shimanouchi, Naoya Shimauchi, Hisashi Yagi, Hiroshi Umakoshi, Yuji Goto, Ryoichi Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   109 ( 6 )   629 - 634   2010.6

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    The inhibition of fibril formation of amyloid beta proteins (A beta) would be attractive therapeutic targets for the treatment of Alzheimer's disease (AD). Dopamine (DA) and other catechol derivatives were used as inhibitory factors for A beta fibril formation. The fibril formation of A beta was monitored by Thioflavin T fluorescence, a transmission electron microscopy (TEM) and a total internal reflection fluorescence microscopy (TIRFM). Catechol and its derivatives showed the dose-dependent inhibitory effects on the spontaneous A beta fibril formation. The inhibitory activity depended on the chemical structure of catechol derivatives both in the presence and absence of the liposome a model of biomembrane. Formation of catechol quinone-conjugated-A beta adduct by a Schiff-base is a key step for the inhibition effect of A beta fibril formation. (C) 2009, The Society for Biotechnology, japan. All rights reserved.

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  • Laser-induced Propagation and Destruction of Amyloid beta Fibrils

    Hisashi Yagi, Daisaku Ozawa, Kazumasa Sakurai, Toru Kawakami, Hiroki Kuyama, Osamu Nishimura, Toshinori Shimanouchi, Ryoichi Kuboi, Hironobu Naiki, Yuji Goto

    JOURNAL OF BIOLOGICAL CHEMISTRY   285 ( 25 )   19660 - 19667   2010.6

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    The amyloid deposition of amyloid beta (A beta) peptides is a critical pathological event in Alzheimer disease (AD). Preventing the formation of amyloid deposits and removing preformed fibrils in tissues are important therapeutic strategies against AD. Previously, we reported the destruction of amyloid fibrils of beta 2-microglobulin K3 fragments by laser irradiation coupled with the binding of amyloid-specific thioflavin T. Here, we studied the effects of a laser beam on A beta fibrils. As was the case for K3 fibrils, extensive irradiation destroyed the preformed A beta fibrils. However, irradiation during spontaneous fibril formation resulted in only the partial destruction of growing fibrils and a subsequent explosive propagation of fibrils. The explosive propagation was caused by an increase in the number of active ends due to breakage. The results not only reveal a case of fragmentation-induced propagation of fibrils but also provide insights into therapeutic strategies for AD.

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  • Analysis of the 22-NBD-cholesterol transfer between liposome membranes and its relation to the intermembrane exchange of 25-hydroxycholesterol

    Haruyuki Ishii, Toshinori Shimanouchi, Hiroshi Umakoshi, Peter Walde, Ryoichi Kuboi

    COLLOIDS AND SURFACES B-BIOINTERFACES   77 ( 1 )   117 - 121   2010.5

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    The transfer of 22-NBD-cholesterol (22-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-23,24-bisnor-5-cholen-3-ol) between two liposome membranes was quantitatively analyzed by using the fluorescence resonance energy transfer (FRET) method. Liposomes labeled with both 22-NBD-cholesterol and a rhodamine-labeled phosphatidylethanolamine (Rh-DHPE) were used as donor liposomes, and the 22-NBD-cholesterol transfer from these donor liposomes to acceptor liposomes prepared from same type of phosphatidylcholine was monitored. The transfer kinetics was found to be composed of a fast and a slow phase, and all kinetic measurements could be fitted with a bi-exponential model. The results obtained indicate that the 22-NBD-cholesterol transfer kinetics between liposome membranes depends on the fluidity of the liposome used and that the curvature may affect the kinetics. Furthermore, the behavior of 22-NBD-cholesterol in lipid membrane is similar to that of the oxysterol 25-hydroxycholesterol rather than cholesterol. It is proposed that 22-NBD-cholesterol can be a useful fluorescent probe to mimic the intermembrane transfer of oxidized cholesterols like 25-hydroxycholesterol, rather than that of cholesterol itself. (C) 2010 Elsevier B.V. All rights reserved.

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  • Preparation of superoxide dismutase LIPOzyme in hollow fiber membrane module

    Hiroyuki Sugaya, Hiroshi Umakoshi, K. B. M. A. Fadzil, Le Quoc Tuan, Toshinori Shimanouchi, Ryoichi Kuboi

    DESALINATION AND WATER TREATMENT   17 ( 1-3 )   281 - 287   2010.5

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    Liposome-loaded membrane module (LLM) was prepared in hollow fiber module (HF-LLM), where the liposome was loaded into the hollow fiber membrane. The filtration property of the LLM was characterized. The oxidized and fragmented superoxide dismutase (SOD) was applied to the prepared LLM to separate the peptide to give a SOD-like activity on the liposome membrane, resulting in the recovery of the specific peptide. It was found that the SOD-like activity could be obtained in the SOD LIPOzyme prepared, resulting in the effective elimination of the superoxide in the HF-LLM.

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  • Monitoring of membrane damages by dialysis treatment: Study with membrane chip analysis

    Toshinori Shimanouchi, Ena Oyama, Huong Thi Vu, Haruyuki Ishii, Hiroshi Umakoshi, Ryoichi Kuboi

    DESALINATION AND WATER TREATMENT   17 ( 1-3 )   45 - 51   2010.5

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    The liposome immobilized on indium tin-oxide electrode was prepared as a "Membrane Chip'' to evaluate the liposome-liposome interaction. Three kinds of neutral liposomes entrapping the fluorescence probe, calcein, were utilized as a sensory liposome. Twenty four kinds of liposomes were used to construct a membrane library based on the calcein release behavior. The loading of liposome including the unsaturated phospholipid into the dialyzer induced the variation of surface state of liposome membrane. We analyzed the above liposome by a comparison of it with the membrane library constructed by Membrane Chip. The liposome after its dialysis treatment was found to show the membrane property of the liposome with domain-like structure prior to the interaction with amyloid beta protein. Also the above liposome showed the oxidized liposome-like membrane property. In conclusion, we demonstrated a membrane library-based method to evaluate the surface state of the model biomembranes with unclear surface property.

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  • Liposomes destabilize tRNA during heat stress

    Keishi Suga, Hiroshi Umakoshi, Hibiki Tomita, Tomoyuki Tanabe, Toshinori Shimanouchi, Ryoichi Kuboi

    BIOTECHNOLOGY JOURNAL   5 ( 5 )   526 - 529   2010.5

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    Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine) and cholesterol (Ch). To compare thermal stability, midpoint temperature (T(m)) of tRNA was calculated from normalized theta(208). Addition of POPC/Ch liposomes decreased the T(m) value of tRNA from 48 degrees C to 38 degrees C. We conclude that POPC/Ch liposomes interact with tRNA and destabilize its conformation under heat stress.

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  • A sensitive thermochemical detector with a new target droplet supply and immobilization process

    M. Noda, T. Shimanouchi, T. Asai, K. Yamashita, M. Okuyama, H. Umakoshi, R. Kuboi

    SENSORS AND ACTUATORS B-CHEMICAL   147 ( 1 )   337 - 342   2010.5

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    A thermal detector structure of bio-thermochemical sensor with a new target droplet supply is considered, analyzed and developed. The basic structure of the bio-thermochemical sensor is a Pt film microbolometer thermally insulated from a silicon thermal conductor substrate to enhance the temperature sensitivity. In the detector structure, it is calculated from a finite element analysis that the variation in lateral size of immobilized droplet, larger than detector film size of 50 mu m, affects little on the temperature change, while the real vertical size of the droplet keeps nearly the same as around 70 mu m. These indicate the validity of formation of the target droplet onto the detector part as an accurate thermal measurement. Based on this, a new supplying and immobilizing process of the droplet is developed to realize a soft formation of the droplet on the detector surface with retained intact immobilization of liposome at least for 24 h, not giving the membrane a mechanical stress and destruction.
    Thereafter, we have evaluated a bio-thermochemical reaction in a droplet of mixed solution of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposome and lysozyme by the newly developed thermochemical sensor. Some new peaks in thermal spectra were observed for the case of the mixture, which were not found so far in the case of each single solution of liposome or lysozyme, indicating that their bio-thermochemical interactions were newly detected in this method. It was also found that the thermal sensor with new forming process of droplet target detects the thermochemical reaction of respective DPPC liposome and lysozyme, consistent with the same as those reported by a differential scanning calorimetry. (C) 2010 Elsevier B.V. All rights reserved.

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  • Development of liposome-based mimics of superoxide dismutase and peroxidase based on the "LIPOzyme" concept

    Hiroshi Umakoshi, Kengo Morimoto, Naoto Yasuda, Toshinori Shimanouchi, Ryoichi Kuboi

    JOURNAL OF BIOTECHNOLOGY   147 ( 1 )   59 - 63   2010.5

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    An antioxidative liposome catalyst. LIPOzyme, that mimics both superoxide dismutase (SOD) and peroxidase (POD)-like activities has been developed by using liposomes modified with simple ligands (dodecanoyl-histidine, Dodec-His) and metal ions (Mn) The SOD-like activity is dependent on the stability of the ligand-metal complex on the liposome membrane, with the value being higher for the DPPC liposome and at a higher pH The POD-like activity was found to be maximal in the case of DMPC liposome, in which the ligand-metal complex is inserted more deeply into the membrane. It was thus shown that liposome modified with simple ligands can exhibit different enzyme-like activities depending on the characteristics of the liposome membrane. (C) 2010 Elsevier B V All rights reserved.

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  • As-Lipozyme: Characterization of Its Activity Like Antioxidative Enzymes

    L. Q. Tuan, T.T.T. Huong, H. Umakoshi, T. Shimanouchi, R. Kuboi, K. Shiomori, Y. Baba

    Proc. 2nd International Symposium on Health Hazards of Arsenic Contamination   138 - 142   2010.5

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  • Chitosanase displayed on liposome can increase its activity and stability

    Kien Xuan Ngo, Hiroshi Umakoshi, Toshinori Shimanouchi, Hiroyuki Sugaya, Ryoichi Kuboi

    JOURNAL OF BIOTECHNOLOGY   146 ( 3 )   105 - 113   2010.4

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    The strategy to prepare a novel biocatalyst by the immobilization of chitosanase onto liposome (ICL) was carried out based on the direct interaction of liposomes with cell membrane of Streptomyces griseus cell. The ICL was characterized in relation to the molecular weight of protein, the chitosanase activity, the effect of the surface hydration of various liposomes on hydrolysis activity of immobilized chitosanase and the stability of ICL under various extreme conditions. The SOS-PAGE analysis of the purified ICL sample shows the existence of a protein with approximately 39 kDa that corresponded to the sum of weight of the mature chitosanase and its signal peptide (38.8 kDa). The above protein of ICL also expresses the chitosanase activity that is significantly higher than that of the conventional chitosanase. Furthermore, the surface hydration of liposomes used to prepare ICL that affected the activity of immobilized chitosanase verified the importance of liposome surfaces. Indeed, the stability of ICL assayed by measuring the chitosanase activity is significantly higher than that of conventional chitosanase under various temperatures and pH conditions. These characteristics of ICL show the possible preparation of the biocatalysts that can be prepared by immobilizing enzymes onto liposome vesicles properly. (C) 2010 Elsevier B.V. All rights reserved.

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  • A beta/Cu-catalyzed oxidation of cholesterol in 1,2-dipalmitoyl phosphatidylcholine liposome membrane

    Toshinori Shimanouchi, Makoto Tasaki, Huong Thi Vu, Haruyuki Ishii, Noriko Yoshimoto, Hiroshi Umakoshi, Ryoichi Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   109 ( 2 )   145 - 148   2010.2

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    The amyloid beta protein with 42 amino acid residues (A beta), which is a causative protein of Alzheimer's disease (AD), forms the complex with copper (II) to induce the cholesterol oxidase-like activity by the proton transfer from the cholesterol. In this study, the oxidation of cholesterol by A beta/Cu complex was investigated on the surface of the zwitterionic phospholipid liposome including the bound water advantageous for the enhancement of the proton transfer. The bound water was pooled by the formation of cholesterol-rich domain within liposomes. The resulting reactivity was enhanced by the proton transfer mediated by the bound water. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

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  • Sensitivity Enhancement of Leakage Current Microsensor for Detection of Target Protein by Using Protein Denaturant

    P. Lorchirachoonkul, K. Yamashita, M. Noda, T. Shimanouchi, H. Umakoshi, R. Kuboi

    2010 IEEE SENSORS   1056 - 1059   2010

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    We have developed a highly-sensitive leakage current microsensor by using 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) liposome entrapping K-4[Fe(CN)(6)] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states and we can use this leakage current microsensor to detect other protein that do not have large interaction with the liposome.

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  • Characterization of Amyloid beta Fibrils with An Aqueous Two-Phase System: Implications of Fibril Formation

    Toshinori Shimanouchi, Naoya Shimauchi, Keiichi Nishiyama, Huong Thi Vu, Hisashi Yagi, Yuji Goto, Hiroshi Umakoshi, Ryoichi Kuboi

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   17   121 - 128   2010

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    The pathological process of Alzheimer's disease is closely related to amyloid fibril formation by the causative protein, amyloid beta (A beta). The growth behavior of A beta fibrils is predominated by the seeds-monomeric A beta interaction. In this study, the local hydrophobicity of seeds of A beta fibrils was investigated by the aqueous two-phase partitioning method to evaluate the hydrophobic interaction between the seed-monomeric A beta. The seeds showed a high local hydrophobicity relative to the monomer and fibrils. From the fibril growth experiment and the additive effect of Triton X-100, we could demonstrate the contribution of the hydrophobic seeds-monomer interaction to fibril formation.

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  • A Highly-Sensitive Leakage Current Microsensor by Using Denaturant: Detection of Target Protein by DPPC Liposome

    P. Lorchirachoonkul, T. Shimanouchi, K. Yamashita, H. Umakoshi, R. Kuboi, M. Noda

    EUROSENSORS XXIV CONFERENCE   5   812 - 815   2010

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    We have developed a highly-sensitive leakage current microsensor by using the DPPC liposome entrapping K-4[Fe(CN)(6)] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully obtain a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine ( CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states. (C) 2009 Published by Elsevier Ltd.

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  • 固体界面における脂質膜のストレス応答~ ベシクル成長・形態変化~

    島内 寿徳, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2010   665 - 665   2010

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  • アミロイドβタンパク質の生体膜晶析:金属イオン共存効果

    島内 寿徳, 大西 諒, 北浦 奈知, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2010   988 - 988   2010

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  • リポソームによるヘキソキナーゼ活性の制御

    馬越 大, 西田 惇史, 島内 寿徳

    化学工学会 研究発表講演要旨集   2010   989 - 989   2010

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  • リポソーム固定化膜モジュールによるアミロイド性タンパク質の分離

    馬越 大, Prasertsuk Kiattisak, 菅谷 博之, 島内 寿徳

    化学工学会 研究発表講演要旨集   2010   1050 - 1050   2010

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  • 生体膜干渉:リポソーム膜上におけるRNA相互作用および翻訳の制御

    菅 恵嗣, 田部 智之, 富田 響, 島内 寿徳, 馬越 大

    化学工学会 研究発表講演要旨集   2010   476 - 476   2010

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  • メンブレンチップによる膜間相互作用の評価

    島内 寿徳, 萬野 雄也, 大山 恵奈, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2010   981 - 981   2010

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  • リポソーム共存下におけるRNAの構造変化の解析とその応用

    馬越 大, 富田 響, 菅 恵嗣, 島内 寿徳

    化学工学会 研究発表講演要旨集   2010   987 - 987   2010

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  • ストレスによるDOTAPリポソームの膜特性変化を利用したin vitro GFP遺伝子発現の制御

    馬越 大, 田部 智之, 菅 恵嗣, 富田 響, 島内 寿徳

    化学工学会 研究発表講演要旨集   2010   986 - 986   2010

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  • 脂質膜界面の自己組織化を利用する機能場の創出:プロテオリポソーム

    島内 寿徳, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2010   211 - 211   2010

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  • アミロイド性タンパク質の伸長挙動におよぼす酸化リポソームの影響

    島内 寿徳, 北浦 奈知, 大西 諒, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2010   1101 - 1101   2010

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  • Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome

    Kien Xuan Ngo, Hiroshi Umakoshi, Haruyuki Ishii, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108 ( 6 )   471 - 476   2009.12

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    The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 degrees C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

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  • Membranomics Research on Interactions between Liposome Membranes with Membrane Chip Analysis

    SHIMANOUCHI Toshinori, OYAMA Ena, ISHII Haruyuki, UMAKOSHI Hiroshi, KUBOI Ryoichi

    MEMBRANE   34 ( 6 )   342 - 350   2009.11

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    The liposome immobilized on indium tin–oxide (ITO) electrode was prepared to evaluate the liposome–liposome interaction to develop "Membrane Chip". Three kinds of standard liposomes entrapping the fluorescence probe, calcein, were used for their immobilization, where the immobilized liposomes show the different characteristics in their phase transition temperature and membrane fluidity. Membrane Chip could give the set of the quantitative information relating to the membrane–membrane interaction. The interaction between three immobilized liposomes and 22 kinds of liposomes as samples was then systematically investigated on a basis of the calcein release from the immobilized liposomes, which could give the information in relation to "Membranome". It was, therefore, found that the Membrane Chip as a means of Membranomics research was effective to evaluate the Membranome information of liposomes.

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  • Charged liposome affects the translation and folding steps of in vitro expression of green fluorescent protein

    Hiroshi Umakoshi, Keishi Suga, Huong Thi Bui, Masato Nishida, Toshinori Shimanouchi, Ryoichi Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108 ( 5 )   450 - 454   2009.11

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    The role of the charged liposome on the in vitro expression of green fluorescent protein (GFP) was investigated, focusing on its elemental steps such as transcription, translation and folding. The total GFP expression was enhanced to 145% when a neutral liposome (POPC: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocoline) was added externally to a cell-free translation system. On the contrary, the addition of the charged liposome composed of POPC with anionic 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (POPG) or cationic stearyl amine (SA) inhibited the total GFP expression, depending on the surface charge density of liposome. In transcription, the RNA synthesis was enhanced regardless of the variation of the surface charge, indicating that transcription was enhanced due to the stabilization of RNA structure by its hydrophobic interaction with liposome. Translation was inhibited by cationic liposome although it was enhanced by anionic liposome and neutral liposome. On the other hand, the folding was not inhibited in the presence of neutral liposome, whereas anionic liposome and cationic liposome inhibited the folding in proportion to the their surface charges, suggesting that the total GFP expression was controlled by a charged liposome in the translation step and folding step. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

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  • Analysis of interaction between liposome membranes induced by stress condition: Utilization of liposomes immobilized on indium tin oxide electrode Reviewed

    Haruyuki Ishii, Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108 ( 5 )   425 - 428   2009.11

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    NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with alpha-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

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  • Preparation of liposome immobilized membrane module and its application Reviewed

    Hiroshi Umakoshi, Hiroyuki Sugaya, Yuji Tohtake, Toshinori Shimanouchi, Ryoichi Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108   S65 - S65   2009.11

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  • Membrane interference: Role of liposome on in vitro gene expression of GFP Reviewed

    Suga Keishi, Umakoshi Hiroshi, Bui Huong Thi, Tanabe Tomoyuki, Shimanouchi Toshinori, Kuboi Ryoichi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108   S102 - S103   2009.11

  • Development of membrane chip system for study on membrane-protein interaction Reviewed

    Shimanouchi Toshinori, Ishii Haruyuki, Oyama Ena, Shimauchi Naoya, Umakoshi Hiroshi, Kuboi Ryoichi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108   S162 - S163   2009.11

  • Disaggregation of catecholamine derivative-induced amyloid fibrils on biomembranes Reviewed

    Shimanouchi Toshinori, Vu Huong Thi, Umakoshi Hiroshi, Kuboi Ryoichi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108   S31 - S32   2009.11

  • Calcein permeation across phosphatidylcholine bilayer membrane: Effects of membrane fluidity, liposome size, and immobilization

    Toshinori Shimanouchi, Haruyuki Ishii, Noriko Yoshimoto, Hiroshi Umakoshi, Ryoichi Kuboi

    COLLOIDS AND SURFACES B-BIOINTERFACES   73 ( 1 )   156 - 160   2009.10

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    The permeation of calcein across the phospholipid bilayer membrane is a key phenomenon in the detection system using liposomes as a sensor unit. The behavior of the calcein release from the liposome was analyzed by a first-order kinetic to obtain the permeability coefficient, P(s) [cm/s]. The P(s) value for the neutral liposome, prepared by zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), was found to depend on both the diameter of liposome and the temperature. The membrane fluidity of the POPC liposome, evaluated by the hydrophobic probe, 1-(4-trimethyl-aminophenyl)-6-diphenyl-1,3,5-hexatriene, was also dependent on the liposome diameter and the temperature. The P, values for various neutral liposomes under gel phase or liquid-crystalline phase were correlated with their membrane fluidity, although some data were a little scattered, possibly due to the lamellarity. It is therefore considered that the membrane fluidity dominates the permeability of calcein across the neutral phospholipid membrane. Based on the above results, the P, value for liposomes immobilized on the solid surface is discussed. (C) 2009 Elsevier B.V. All rights reserved.

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  • Characterization of heat-induced interaction of neutral liposome with lipid membrane of Streptomyces griseus cell

    Kien Xuan Ngo, Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi

    COLLOIDS AND SURFACES B-BIOINTERFACES   73 ( 2 )   399 - 407   2009.10

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    The interaction between the neutral 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes and cell membrane of Streptomyces griseus induced by the heat treatment at specific temperature was investigated, focusing on the internalization of the neutral POPC liposomes with S. griseus cells. In an attempt to clarify the modes of liposome internalization, various kinds of inhibitors of endocytotic pathways were used to treat S. griseus cells. The efficiency of the heat treatment on liposome-cell membrane interactions was finally characterized based on the hydrophobic, electrostatic interactions and hydration effect. In fact, the internalization of the neutral liposomes into these cells was found to show higher rate and greater amount at higher temperatures. The kinetic study showed that the maximum amount of the internalized liposomes was, respectively, 469 x 10(5) and 643 x 10(5) liposomes/cell at 37 and 41 degrees C. The internalization of the neutral liposomes induced by the heat treatment was characterized, implying that the endocytosis occurred. The interactions involving the internalization, adsorption, and fusion of these liposomes with S. griseus cells were mainly contributed by the hydrophobic interaction and the unstable hydrogen bonds caused by the loss of water of surface hydration of cell membrane rather than the electrostatic interaction under the specific heat condition. (c) 2009 Elsevier B.V. All rights reserved.

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  • Negatively charged liposome as a potent inhibitor of post-translation during in vitro synthesis of green fluorescent protein

    Huong Thi Bui, Hiroshi Umakoshi, Keishi Suga, Masato Nishida, Toshinori Shimanouchi, Ryoichi Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL   46 ( 2 )   154 - 160   2009.10

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    The effect of negatively charged liposome on in vitro synthesis of a reporter protein, green fluorescent protein (UP), was investigated using a cell-free translation system. GFP was expressed with and without negatively charged liposome prepared with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylchorine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), resulting in the GFP fluorescence being reduced to approximately 60% in the presence of POPC/POPG at more than 30% POPG, depending oil its concentration. However, the amount of synthesized GFP products, as analyzed by SDS-PAGE, did not change with and without the POPC/POPG liposome. The results of the ultrafiltration operation indicate that the liposome interacts not only with synthesized polypeptide GFP but also with folded synthesized GFP (mature GFP). Liposome also inhibited refolding of unfolded GFP to its native state. The above results show that the POPC/POPG could inhibit the folding of GFP in the post-translational process of the gene expression product. (C) 2009 Elsevier B.V. All rights reserved.

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  • Polymethylthiophene/Nafion-modified glassy carbon electrode for selective detection of dopamine in the presence of ascorbic acid

    Vu Thi Huong, Toshinori Shimanouchi, Do Phuc Quan, Hiroshi Umakoshi, Pham Hung Viet, Ryoichi Kuboi

    JOURNAL OF APPLIED ELECTROCHEMISTRY   39 ( 10 )   2035 - 2042   2009.10

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    The possible use of an electrode modified with electroactive conductive poly(3-methylthiophene) (PMeT)/Nafion as a chemical sensor was investigated for the voltammetric analysis of Dopamine (DA). The electrochemical behavior of dopamine was examined by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. By using a PMeT-modified glassy carbon (GC/PMeT) electrode, DA and Ascorbic Acid (AA) signals could be separated but the AA at high concentrations still caused significant interference by overlapping the DA peak. In comparison to the GC/PMeT electrode, the glassy carbon (GC/Nafion/PMeT) electrode modified with hybrid film Nafion/PMeT was found to permit a superior separation by shifting the oxidation of AA peak toward the less positive potential. The DPV curves for a mixture of DA and AA at an GC/Nafion/PMeT electrode in a 0.1 M H(2)SO(4) solution showed peaks of DA and AA, at 0.45 and 0.21 V, respectively, indicating that the difference in the oxidation potential was 240 mV. In the 0.1 M H(2)SO(4) solution, the oxidation peak current on the differential pulse voltammograms for the GC/PMeT electrode increased linearly with the concentration of DA in the range 1 x 10(-6) to 1 x 10(-3) M, and the oxidation peak current on the differential pulse voltammograms for the GC/Nafion/PMeT electrode in the range 5 x 10(-7) to 2 x 10(-4) M. The DA detection sensitivity of the GC/Nafion/PMeT electrode (26.7 mu A mu M(-1) cm(-2)) was 22 times higher than that of the GC/PMeT electrode (1.21 mu A mu M(-1) cm(-2)).

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  • Preparation of Hollow Fiber Immobilized Liposome Membrane

    SUGAYA Hiroyuki, UMAKOSHI Hiroshi, TOHTAKE Yuji, OYAMA Ena, SHIMANOUCHI Toshinori, KUBOI Ryoichi

    MEMBRANE   34 ( 5 )   272 - 280   2009.9

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    Immobilized liposome membrane (ILM) was prepared in hollow fiber module (HF–ILM), where the liposome was loaded into the hollow fiber membrane module and was physically immobilized by forming the hydrogel in the cavity of the fiber membrane. Basic nature of the hydrogels prepared with xanthan gum (XG) and polyethyleneimine (PEI) via amino coupling method was first investigated in relation to the water contents of the gel matrix, so that the optimal condition for the liposome immobilization was obtained. The HF–ILM was prepared at the optimal condition, resulting in the high contents of immobilized liposome with high stability.

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  • Immobilization of intact liposomes on solid surfaces: A quartz crystal microbalance study

    Thi Huong Vu, Toshinori Shimanouchi, Haruyuki Ishii, Hiroshi Umakoshi, Ryoichi Kuboi

    JOURNAL OF COLLOID AND INTERFACE SCIENCE   336 ( 2 )   902 - 907   2009.8

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    Immobilization of model cell membranes (liposomes) on sensing devices is useful for the detection, evaluation, and analysis of interaction between cell membranes and proteins or other biological materials. The method to immobilize the liposomes on the solid surface was investigated by using the quartz crystal microbalance (QCM) method. focusing on the density of immobilized liposomes and their stability. The different liposomes were applied onto the planar surface of the QCM electrode by varying the immobilization method. The results showed that the immobilized liposomes depended not only on the immobilization method but also on the properties of the liposomes. The liposome with low membrane fluidity likely showed intact immobilization on a solid surface. An electrostatic interaction also affected the amount of immobilized liposomes on the functionalized quartz crystal. By using the amino-coupling method, intact liposomes could be immobilized on a solid surface and immobilized liposomes could stabilize for 10 h with frequency changes less than 5%. (C) 2009 Elsevier Inc. All rights reserved.

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  • Inversion of the Configuration of a Single Stereocenter in a beta-Heptapeptide Leads to Drastic Changes in its Interaction with Phospholipid Bilayers

    Toshinori Shimanouchi, Peter Walde, James Gardiner, Stefania Capone, Dieter Seebach, Ryoichi Kuboi

    CHEMBIOCHEM   10 ( 12 )   1978 - 1981   2009.8

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    In summary, a change in the configuration of a single amino acid in a β3-heptapeptide can lead to remarkable changes in the physicochemical properties of the peptide with respect to the interaction with a (protein-free) biomimetic environment. Thus, the presence of an unnatural amino acid configuration in a peptide can have consequences not only with respect to specific interactions with proteins, but also, indirectly, with respect to the interaction with membrane lipids. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

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  • Kinetic Study on Giant Vesicle Formation with Electroformation Method

    Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

    LANGMUIR   25 ( 9 )   4835 - 4840   2009.5

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    Giant vesicles (GVs) composed of zwitterionic phospholipids were prepared by the electroformation method. The growth behavior of GVs was quantitatively analyzed as a first-order kinetics of the radius of GVs to obtain the apparent growth rate constant k(Gr). On the basis of the dependence of the k(Gr) value on the preparation temperature and the lipid composition of GVs, the membrane fluidity of vesicle membranes was found to be dominant in the growth behavior of GVs. The comparison of the k(Gr) values with the membrane fluidity for GVs suggested the validity of the model that the swelling of the lipid membrane could induce the growth of GVs.

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  • Cationic Liposome Inhibits Gene Expression in an E. coli Cell-Free Translation System Reviewed

    Bui, H. T, Umakoshi, H, Suga, K, Tanabe, T, Shimanouchi, T, Kuboi, R

    Membrane   34 ( 3 )   146 - 151   2009.3

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  • Liposome membrane can act like molecular and metal chaperones for oxidized and fragmented superoxide dismutase

    Le Quoc Tuan, Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi

    ENZYME AND MICROBIAL TECHNOLOGY   44 ( 2 )   101 - 106   2009.2

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    A mechanism for liposome-recruited activity of oxidized and fragmented superoxide dismutase (Fr.-SOD) [Tuan LQ, Umakoshi H, Shimanouchi T, Kuboi R. Liposome-recruited activity of oxidized and fragmented superoxide dismutase. Langmuir 2008;24:350-4] was further investigated, focusing on the secondary structure of Fr.-SOD. Liposome membrane was found to assist the conformational change of Fr.-SOD and reactivate the enzymatic activity. like molecular and metal chaperones. The loss of SOD activity and its secondary structure was observed during 6 h oxidation in 2 mM hydrogen peroxide. The contents of the alpha-helix and beta-sheet structures in the oxidized and fragmented SOD (2 mu M) were increased only in the presence of 10 mu M Cu2+ and Zn2+ together, or in the presence of 2 mM POPC liposomes. The mixture of all of these elements (fragmented SOD and POPC liposomes with Cu2+ and Zn2+) gave not only the increase of the alpha-helix and beta-sheet contents but also the mediation of the high SOD-like activity. (c) 2008 Published by Elsevier Inc.

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  • A Microbolometer of Bio-Thermochemical Sensor Immobilized Lipo some for Detection of Causative Protein of Alzheimer's Disease, Amyloid Beta

    M. Noda, T. Asai, T. Shimanouchi, K. Yamashita, H. Umakoshi, M. Okuyama, R. Kuboi

    PROCEEDINGS OF THE EUROSENSORS XXIII CONFERENCE   1 ( 1 )   1071 - +   2009

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    Forming process of liposome droplet and its stable immobilization on a Si-based microbolometer are investigated and applied for detection of causative protein of Alzheimer's disease, amyloid beta (A beta) protein, where the disease is closely related to the amyloid fibril formation. This work is a preliminary but innovative approach applying a bio-thermochemical microsensor to monitor the formation. It is observed that the bolometer surface keeps a long time intact behavior of the liposome up to 24 hours. It seems from time course results with A beta that the variation in the temperature profile corresponds to that observed in fluorescence analysis result of the formation.

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  • A Leakage Current Microsensor for Detection of Interaction between an Electrolyte-Entrapping Liposome and Protein

    Minoru Noda, Takeshi Asai, Kaoru Yamashita, Toshinori Shimanouchi, Hiroshi Umakoshi, Masanori Okuyama, Ryouichi Kuboi

    2009 IEEE SENSORS, VOLS 1-3   1883 - +   2009

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    We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K-4[Fe(CN)(6)] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

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  • Characterization of Hollow Fiber Immobilized Liposome Membrane by Using Aqueous Two-Phase Partitioning Systems

    Hiroyuki Sugaya, Hiroshi Umakoshi, Yuji Tohtake, Toshinori Shimanouchi, Ryoichi Kuboi

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   16   103 - 109   2009

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    The modification of the surface of a polysulfone (PSf) membrane in a hollow fiber module was performed based on the evaluated hydrophobicity of the polymers used in its modification to develop a new type of membrane module immobilizing liposome. The surface net hydrophobicity of the polyvinylpyrrolidone (PVP), to be modified, was first characterized by using the aqueous two-phase partitioning method. The hollow fiber module using the PVP-modified PSf membrane was characterized based on the adsorption behaviors of standard amino acids, showing that the membrane surface was found to have a hydrophilic surface nearly equal to the difference in aqueous two-phase systems. The immobilized liposome membrane (ILM) in the above module was found to have a hydrophobic potential, thus promoting the adsorption of the hydrophobic molecules on its surface.

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  • Characterization of Green Fluorescent Protein Using Aqueous Two-Phase Systems

    Hiroshi Umakoshi, Masato Nishida, Keishi Suga, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   16   145 - 150   2009

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    The surface net hydrophobicity and local hydrophobicity of green fluorescent protein was evaluated by using the aqueous two-phase partitioning method. The surface net hydrophobicity of GFP allowed it to be classified as a hydrophobic protein although the local hydrophobicity was low. The local hydrophobicity of the GFP was increased by a pH shift to acidic conditions and was maximal at pH 4. The surface net hydrophobicity increased at acidic conditions less than pH 4. The conformational change of GFP at acidic pH is discussed based on the above results.

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  • A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein

    M. Noda, T. Asai, T. Shimanouchi, K. Yamashita, H. Umakoshi, M. Okuyama, R. Kuboi

    PROCEEDINGS OF THE EUROSENSORS XXIII CONFERENCE   1 ( 1 )   1295 - +   2009

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    We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K-4[Fe(CN)(6)] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

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  • A Microbolometer of Bio-Thermochemical Sensor Immobilized Lipo some for Detection of Causative Protein of Alzheimer's Disease, Amyloid Beta

    M. Noda, T. Asai, T. Shimanouchi, K. Yamashita, H. Umakoshi, M. Okuyama, R. Kuboi

    PROCEEDINGS OF THE EUROSENSORS XXIII CONFERENCE   1 ( 1 )   1071 - +   2009

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    Forming process of liposome droplet and its stable immobilization on a Si-based microbolometer are investigated and applied for detection of causative protein of Alzheimer's disease, amyloid beta (A beta) protein, where the disease is closely related to the amyloid fibril formation. This work is a preliminary but innovative approach applying a bio-thermochemical microsensor to monitor the formation. It is observed that the bolometer surface keeps a long time intact behavior of the liposome up to 24 hours. It seems from time course results with A beta that the variation in the temperature profile corresponds to that observed in fluorescence analysis result of the formation.

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  • A Bio-Thermochemical Sensor of Microbolometer Immobilized Liposome for Detection of Causative Protein of Alzheimer's Disease, Amyloid Beta

    Minoru Noda, Takeshi Asai, Kaoru Yamashita, Toshinori Shimanouchi, Hiroshi Umakoshi, Masanori Okuyama, Ryouichi Kuboi

    2009 IEEE SENSORS, VOLS 1-3   838 - +   2009

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    Forming process of Liposome droplet and its stable immobilization on a Si-based microbolometer are investigated and applied for detection of causative protein of Alzheimer's disease, amyloid beta (A beta) protein, where the disease is closely related to the amyloid fibril formation. This work is a preliminary but innovative approach applying a bio-thermochemical microsensor to monitor the formation. It is observed that the bolometer surface keeps a long time intact behavior of the liposome up to 24 hours. It seems from time course results with A beta that the variation in the temperature profile corresponds to that observed in fluorescence analysis result of the formation.

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  • A Leakage Current Microsensor for Detection of Interaction between an Electrolyte-Entrapping Liposome and Protein

    Minoru Noda, Takeshi Asai, Kaoru Yamashita, Toshinori Shimanouchi, Hiroshi Umakoshi, Masanori Okuyama, Ryouichi Kuboi

    2009 IEEE SENSORS, VOLS 1-3   1883 - +   2009

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    We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K-4[Fe(CN)(6)] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

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  • リポソームの物質透過に及ぼす界面物性の影響

    島内 寿徳, 石井 治之, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2009   140 - 140   2009

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  • LIPOzyme~リポソームの分子認識能を利用する人工酵素の開発

    馬越 大, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2009   946 - 946   2009

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  • リポソームによるアミロイド多形性の制御

    島内 寿徳, 嶋内 直哉, 大西 諒, 西山 圭一, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2009   1018 - 1018   2009

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  • 酸化劣化リポソームによるAβ超分子集合体形成の制御

    島内 寿徳, 大西 諒, 嶋内 直哉, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2009   1017 - 1017   2009

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  • Membrane on Membrane~Liposome固定化膜モジュールの開発と応用

    馬越 大, 菅谷 博之, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2009   646 - 646   2009

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  • 生体膜上におけるアミロイド性タンパク質の晶析現象

    島内 寿徳, 嶋内 直哉, 大西 諒, Vu Thi Huong, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2009   741 - 741   2009

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  • 固定化リポソーム-QCMを用いたタンパク質の分子内水素結合安定性の評価

    島内 寿徳, Vu Thi Huong, 石井 治之, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2009   715 - 715   2009

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  • 抗酸化SOD/Catalase LIPOzymeの機能評価

    馬越 大, 森本 研吾, Le Quoc Tuan, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2009   680 - 680   2009

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  • リポソームによるアミロイド線維形成の制御

    島内 寿徳, 嶋内 直哉, 西山 圭一, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2009   714 - 714   2009

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  • リポソームによるIn vitro GFP発現の制御

    馬越 大, 菅 恵嗣, Bui Thi Huong, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2009   679 - 679   2009

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  • Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress

    Kien Xuan Ngo, Hiroshi Umakoshi, Toshinori Shimanouchi, Huong Thi Bui, Ryoichi Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   106 ( 6 )   602 - 605   2008.12

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    A direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes with membrane of Streptomyces griseus cell under the heat stress at 41 degrees C increased the chitosanase production and release to 2.2 times higher than that at 37 degrees C without the POPC liposomes. Amount of chitosanase released across the lipid mimicking cell membrane (LMCM) liposome under a heat at 41 degrees C in the presence of POPC liposomes was 17% of initially-entrapped chitosanase while it was only 1% in the absence of POPC liposomes, even under a heat stress at 41 degrees C, clearly showing the importance of the direct interaction between membrane and membrane.

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  • A bio-thermochemical microbolometer with immobilized intact liposome on sensor solid surface Reviewed

    M. Noda, T. Shimanouchi, M. Okuyama, R. Kuboi

    SENSORS AND ACTUATORS B-CHEMICAL   135 ( 1 )   40 - 45   2008.12

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    We have developed a scheme of bio-thermochemical microbolometer with immobilized intact liposome on sensor solid surface. A liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) was confirmed to be deposited intact by X-ray diffraction (XRD) and AFM analysis on a Pt film that is used as resistive bolometer material. By using a fabricated surface micromachined thermal cavity structure beneath the Pt resistor film with the immobilized intact liposome, thermochemical reactions of DPPC on phase transition among gel, ripple and fluid phases have been successfully detected. It is also found from analysis of fluorescence polarization that endothermic reaction especially at around 40 degrees C (phase change from ripple to fluid) corresponds to the change in mobility of phospholipid molecule. As a result we believe that the developed scheme of the microbolometer is effective for detecting thermochemical reaction of biochemical molecules that interact with the liposome. (c) 2008 Elsevier B.V. All rights reserved.

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  • Liposome membrane itself can affect gene expression in the Escherichia coli cell-free translation system

    Huong Thi Bui, Hiroshi Urnakoshi, Kien Xuan Ngo, Masato Nishida, Toshinori Shimanouchi, Ryoichi Kuboi

    LANGMUIR   24 ( 19 )   10537 - 10542   2008.10

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    A possible role of a model biomembrane, liposome, in gene expression was investigated by using the cell-free translation system. A reporter protein, green fluorescence protein (GFP), was expressed in vitro with and without liposome prepared with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyl chorine (POPC) and cholesterol (Ch) (5.7 mM lipid concentration). In the presence of POPC/Ch liposome, the fluorescence intensity of produced GFP was found to be 1.67 times higher than that in the control after 18 h of expression. The results of the SDS-PAGE analysis also show the above promotion effect of the liposome on the net expression of the GFP gene (1.58 times more). The amounts of mRNA were found to be promoted to 1.29 times higher than those in the control. The differences among mRNA, net expression of the GFP gene, and GFP fluorescence indicate that the enhanced GFP expression in the presence of POPC/Ch liposome could primarily affect the transcription and translation of the GFP gene among the possible steps of gene expression. The variation of in vitro gene expression with various liposomes also shows that the biomembrane could act as a modulator to split the genotype and phenotype in a biological cell.

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  • Arsenic (V) induces a fluidization of algal cell and liposome membranes

    Le Quoc Tuan, Tran Thi Thanh Huong, Pham Thi Anh Hong, Tomonori Kawakami, Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

    TOXICOLOGY IN VITRO   22 ( 6 )   1632 - 1638   2008.9

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    Arsenate is one of the most poisonous elements for living cells. When cells are exposed to arsenate, their life activities are immediately affected by various biochemical reactions, such as the binding of arsenic to membranes and the substitution of arsenic for phosphate or the choline head of phospholipids in the biological membranes. The effects of arsenate on the life activities of algae Chlorella vulgaris were investigated at various concentrations and exposure times. The results demonstrated that the living activities of algal cells (10(10) cells/L) were seriously affected by arsenate at a concentration of more than 7.5 mg As/L within 24 h. Algal cells and the artificial membranes (liposomes) were exposed to arsenate to evaluate its effects on the membrane fluidization. In the presence of arsenate, the membranes were fluidized due to the binding and substitution of arsenate groups for phosphates or the choline head on the their membrane surface. This fluidization of the biological membranes was considered to enhance the transport of toxicants across the membrane of algal cells. (C) 2008 Published by Elsevier Ltd.

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  • Characterization of Oxidized and Fragmented Superoxide Dismutase Recruited on Liposome Surface

    TUAN Le Quoc, UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori, KUBOI Ryoichi

    MEMBRANE   33 ( 4 )   173 - 179   2008.7

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    The interaction of oxidized and fragmented SOD with liposomes has been reported to induce the original SOD-likeenzymatic activity (L.Q. Tuan et al., Langmuir, 24 (2008) 350-354).The effect of several kinds of liposomes on theirrecruited activity of oxidized and fragmented SOD was first investigated. The addition of zwitterionic liposomes withhigh membrane fluidity or that with positive charge was found to increase the SOD-like activity of fragmented SODalthough the negatively charged liposome has no effect on its activity. The SOD-like activity was found to be relatedto the adsorbed amount of the peptides on the liposome surface. The analyzed characteristics of the peptide, togeth-er with the above findings, imply that the liposome-recruited activity of the fragmented SOD was related with therecognition of the SOD fragment by the liposome caused by the combination of electrostatic and hydrophobic inter-action, and hydrogen bonding between the peptide and liposome membrane.

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  • Superoxide Dismutase-like Activity of Liposomes Modified with Dodecanoyl His and Metal Ions

    UMAKOSHI Hiroshi, TUAN Le Quoc, MORIMOTO Kengo, OHAMA Yuji, SHIMANOUCHI Toshinori, KUBOI Ryoichi

    MEMBRANE   33 ( 4 )   180 - 187   2008.7

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    The liposome modified with simple ligand and metal ions shows the superoxide dismutase-like activity. The mem-brane fluidity of various liposomes modified with the functional ligand (Dodecanoyl-His; Dodec-His) and the cluster-ing of the ligand on the liposome surface were first characterized, showing that the clustering of Dodec-His could beinduced on the liposome surface at gel-phase. The capacity of adsorption of Cu and Zn was found to be increaseddependently on the type of liposome, resulting in the maximal adsorption in liposome prepared by 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at gel state and with higher ligand clustering state. As a result on the SOD-likeactivity of the metal/ligand-modified liposome, the SOD-like activity was found to be induced by using the above lipo-somes although its activity level is not so high.

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  • Liposome modified with Mn-porphyrin complex can simultaneously induce antioxidative enzyme-like activity of both superoxide dismutase and peroxidase

    Hiroshi Umakoshi, Kengo Morimoto, Yuji Ohama, Hideto Nagami, Toshinori Shimanouchi, Ryoichi Kuboi

    LANGMUIR   24 ( 9 )   4451 - 4455   2008.5

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    An antioxidative liposome catalysis that mimics both superoxide dismutase (SOD) and peroxidase (POD) activities has been developed by using the liposomes modified with lipophilic Mn - (5,10,15,20-tetrakis[1-hexadecylpyridium-4-yl]-21H,23H-porphyrin) (Mn - HPyP). The SOD- and POD-like activities of the Mn - HPyP-modified liposome were first investigated by varying the type of phospholipid, such as 1,2-distearyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-dilauroyi-sn-glycero-3-phosphocholine (DLPC). Higher SOD-like activity was obtained in the case of DLPC and DMPC liposomes, in which the ligands were well-dispersed on the membrane in the liquid crystalline phase. The POD-like activity was maximal in the case of DMPC liposome, in which the Mn - HPyP complex was appropriately clustered on the membrane in the gel phase. On the basis of the above results, the co-induction of the SOD and POD activities to eliminate the superoxide and also hydrogen peroxide as a one-pot reaction was finally performed by using the Mn - HPyP-modified DMPC liposome, resulting in an increase in the efficiency of the elimination of both superoxide and hydrogen peroxide.

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  • Liposome-recruited activity of oxidized and fragmented superoxide dismutase

    Le Quoc Tuan, Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi

    LANGMUIR   24 ( 2 )   350 - 354   2008.1

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    The peptide fragment of H2O2-treated Cu,Zn-superoxidedismutase (SOD) was found to be reactivated with liposomes prepared by 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). The fragmentation of SOD was observed by 2 mM H2O2 treatment as well as by SOD inactivation and the loss of an alpha-helix in the neighborhood of its activity center. The H2O2-treated SOD which lost its activity at different incubation times was dramatically reactivated only by adding POPC liposomes resulting in 1.3-2.8 times higher enzymatic activity. The ultrafiltration analysis of H2O2-treated SOD co-incubated with liposomes shows that some specific peptide fragments of the oxidized SOD can interact with POPC liposomes. A comparison of the fractions detected in reverse-phase chromatography shows that specific SOD fragments are able to contribute to the reactivation of oxidized and fragmented SOD in the presence of POPC liposomes. The liposomes can recruit the potentially active fragment of SOD among the lethally damaged SOD fragments to elucidate the antioxidative function.

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  • Variation of surface properties of Streptomyces griseus cells after heat treatment with liposome

    Kien Xuan Ngo, Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   15   133 - 137   2008

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    Surface properties of Streptomyces griseus cells, such as surface net charge and surface net hydrophobicity, were characterized by using the aqueous two-phase system (ATPS) method. It was found that the surface net charge of S. griseus cells was reduced after the POPC liposome-treatment of the cells at 41 degrees C. The surface net hydrophobicities of S. griseus cells and liposome-treated cells cultivated at 41 degrees C were about - 122 and -510 kJ/mol, respectively, evaluated by the ATPS method. The above results show that the variation of the cell surface properties could be obtained by the cell-liposome interaction under heat stress conditions.

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  • Bio-thermochemical sensor with liposome immobilized intact for protein detection using their interaction and membrane dynamics

    Minoru Noda, Takeshi Asai, Kaoru Yamashita, Toshinori Shimanouchi, Masanori Okuyama, Ryouichi Kuboi

    Proceedings of IEEE Sensors   882 - 885   2008

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    We have developed a new scheme of biothermochemical microbolometer with a small amount of droplet solution consisting from liposome and biochemical target molecules. And newly detected bio-thermochemical reaction between 2-dipalmitoyl-sn-glycero-3-phosphocholline) liposome immobilized intact and protein of lysozyme (enzyme) in a droplet on a Pt thin film microbolometer, where they are dissolved with concentration of 30 mM and 30 μ?, respectively, a droplet with volume of about 65 nL is immobilized on the surface of Pt film bolometer. This paper reports our first experimental results showing interaction between the DPPC liposome and lysozyme by the new scheme of biothermochemical sensor. © 2008 IEEE.

    DOI: 10.1109/ICSENS.2008.4716582

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  • Characterization of surface properties of microbial transglutaminase using aqueous two-phase partitioning method

    Hiroshi Umakoshi, Noriko Yoshimoto, Makoto Yoshimoto, Toshinori Shimanouchi, Ryoichi Kuboi

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   15   111 - 115   2008

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    The surface properties of recombinant microbial transglutaminase (MTG) were characterized by varying the pH through the aqueous two-phase system (ATPS) method in order to select the effective refolding pathways. It was found that the MTG activity was lost and intrinsic tryptophan fluorescence was varied at pH values less than 3.8. In this pH region, the surface net charge and surface net hydrophobicity of the MTG, determined by the ATPS method, gradually increased. At around pH 3.5, the local hydrophobicity of the MTG was found to be maximal.

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  • Membrane Chipによる異常生体膜の評価

    石井 治之, 大山 恵奈, 島内 寿徳, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2008   387 - 387   2008

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    DOI: 10.11491/scej.2008.0.387.0

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  • Heat Induced Interaction between Liposome and Cell Membrane of Streptomyses griseus

    Ngo Kien Xuan

    2008   355 - 355   2008

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    DOI: 10.11491/scej.2008.0.355.0

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  • Role of Negatively-Charged Liposome on Gene Expression of GFP

    Bui Thi Huong

    2008   1010 - 1010   2008

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    DOI: 10.11491/scej.2008f.0.1010.0

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  • モデル生体膜によるGFP構造中間体の認識およびRefolding

    馬越 大, 西田 真人, 菅 恵嗣, Bui Thi Huong, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2008   1009 - 1009   2008

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    DOI: 10.11491/scej.2008f.0.1009.0

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  • 膜間移動現象における酸化脂質の影響

    石井 治之, 島内 寿徳, 馬越 大, 久保井 亮一, Peter Walde

    化学工学会 研究発表講演要旨集   2008   1014 - 1014   2008

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    DOI: 10.11491/scej.2008f.0.1014.0

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  • Aβ/Cu錯体によるドーパミンの変換反応に及ぼす脂質膜の影響

    島内 寿徳, 松本 匡晴, 吉岡 紘志, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2008   1017 - 1017   2008

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    DOI: 10.11491/scej.2008f.0.1017.0

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  • アミロイド形成過程と形態に及ぼす脂質膜の効果

    島内 寿徳, 嶋内 直哉, 西山 圭一, 廣岩 梓, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2008   1015 - 1015   2008

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    DOI: 10.11491/scej.2008f.0.1015.0

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  • リガンド修飾リポソームを用いた触媒機能の設計・開発

    馬越 大, 森本 研吾, 安田 直人, Le Quoc Tuan, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2008   1018 - 1018   2008

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    DOI: 10.11491/scej.2008f.0.1018.0

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  • Interaction between Cell Membrane and Liposome Membrane under Heat Stress Condition

    Kien Xuan Ngo

    2008   1011 - 1011   2008

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    DOI: 10.11491/scej.2008f.0.1011.0

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  • 固体基板上へのリポソームの安定な固定化~メンブレンチップの創製~

    島内 寿徳, Vu Thi Huong, 馬越 大, 久保井 亮一, 森田 誠一

    化学工学会 研究発表講演要旨集   2008   671 - 671   2008

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    DOI: 10.11491/scej.2008f.0.671.0

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  • ストレス負荷リポソーム膜による潜在活性ペプチドの選択的分離~抗酸化LIPOzymeの設計開発~

    馬越 大, Le Quoc Tuan, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2008   840 - 840   2008

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    DOI: 10.11491/scej.2008f.0.840.0

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  • リポソーム表面電荷による遺伝子発現プロセス制御

    馬越 大, 菅 恵嗣, Bui Thi Huong, 西田 真人, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2008   1044 - 1044   2008

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    DOI: 10.11491/scej.2008f.0.1044.0

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  • 透析操作による血中膜成分の表面状態への影響~モデル系による検討~

    島内 寿徳, 大山 恵奈, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2008   1012 - 1012   2008

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    DOI: 10.11491/scej.2008f.0.1012.0

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  • アミロイド形成過程と形態に及ぼす酸化劣化脂質膜の効果

    島内 寿徳, 西山 圭一, 廣岩 梓, 嶋内 直哉, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2008   1016 - 1016   2008

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    DOI: 10.11491/scej.2008f.0.1016.0

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  • Permeation of a beta-heptapeptide derivative across phospholipid bilayers

    Toshinori Shimanouchi, Peter Walde, James Gardiner, Yogesh R. Mahajan, Dieter Seebach, Anita Thomae, Stefanie D. Kraemer, Matthias Voser, Ryoichi Kuboi

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES   1768 ( 11 )   2726 - 2736   2007.11

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    Based on a number of experiments it is concluded that the fluorescein labeled beta-heptapeptide fluoresceinyl-NH-CS-(S)-beta(3)hAla-(S)-beta(3)hArg-(R)beta(3)hLeu-(S)- beta(3)hPhe-(S)-beta(3)hAla-(S)-beta(3)hAla-(S)-beta(3)hLys-OH translocates across lipid vesicle bilayers formed from DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine). The conclusion is based on the following observations: (i) addition of the peptide to the vicinity of micrometer-sized giant vesicles leads to an accumulation of the peptide inside the vesicles; (ii) if the peptide is injected inside individual giant vesicles, it is released from the vesicles in a time dependent manner; (iii) if the peptide is encapsulated within sub-micrometer-sized large unilamellar vesicles, it is released from the vesicles as a function of time; (iv) if the peptide is submitted to immobilized liposome chromatography, the peptide is retained by the immobilized DOPC vesicles. Furthermore, the addition of the peptide to calcein-containing DOPC vesicles does not lead to significant calcein leakage and vesicle fusion is not observed. The finding that derivatives of the beta-heptapeptide (S)-beta(3)hAla-(S)-beta(3)hArg-(R)-beta(3)hLeu-(S)-beta(3)hPhe-(S)-beta(3)hAla-(S)-beta(3)hAla-(S)-beta(3)hLys-OH can translocate across phospholipid bilayers is supported by independent measurements using Tb3+-containing large unilamellar vesicles prepared from egg phosphatidylcholine and wheat germ phosphatidylinositol (molar ratio of 9: 1) and a corresponding peptide that is labeled with dipicolinic acid instead of fluorescein. The experiments show that this dipicolinic acid labeled beta-heptapeptide derivative also permeates across phospholipid bilayers. The possible mechanism of the translocation of the particular beta-heptapeptide derivatives across the membrane of phospholipid vesicles is discussed within the frame of the current understanding of the permeation of certain oligopeptides across simple phospholipid bilayers. (c) 2007 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.bbamem.2007.07.011

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  • Immobilized-Liposome Sensor System for Detection of Proteins under Stress Conditions

    JUNG Ho-Sup, ISHII Haruyuki, SHIMANOUCHI Toshinori, UMAKOSHI Hiroshi, KUBOI Ryoichi

    MEMBRANE   32 ( 5 )   294 - 301   2007.9

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    DOI: 10.5360/membrane.32.

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  • Study on Stress-Mediated Behavior and Preparation of Giant Vesicles

    Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

    Giant Vesicles: Perspectives in Supramolecular Chemistry   6   369 - 377   2007.3

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    DOI: 10.1002/9780470511534.ch29

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  • A bio-thermochemical microbolometer with immobilized intact liposome on sensor solid surface

    M. Noda, T. Shimanouchi, M. Okuyama, R. Kuboi

    TRANSDUCERS '07 & EUROSENSORS XXI, DIGEST OF TECHNICAL PAPERS, VOLS 1 AND 2   135   40 - 45   2007

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    We have developed a scheme of bio-thermochemical microbolometer with immobilized intact liposome on sensor solid surface. A liposome of 1,2-dipaimitoyl-sn-glycero-3-phosphocholline (DPPC) was confirmed to be deposited intact by XRD and AFM analysis on a A film that is used as resistive bolometer material. As a primal stage, we believe that thermochemical reactions of DPPC on phase transition among gel, ripple and fluid phases have been successfully detected, by using a fabricated surface micromachined thermal cavity beneath the Pt resistor film.

    DOI: 10.1016/j.snb.2008.07.022

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  • A bio-thermochemical microbolometer with immobilized intact liposome on sensor solid surface

    M. Noda, T. Shimanouchi, M. Okuyama, R. Kuboi

    TRANSDUCERS '07 & EUROSENSORS XXI, DIGEST OF TECHNICAL PAPERS, VOLS 1 AND 2   839 - 842   2007

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    We have developed a scheme of bio-thermochemical microbolometer with immobilized intact liposome on sensor solid surface. A liposome of 1,2-dipaimitoyl-sn-glycero-3-phosphocholline (DPPC) was confirmed to be deposited intact by XRD and AFM analysis on a A film that is used as resistive bolometer material. As a primal stage, we believe that thermochemical reactions of DPPC on phase transition among gel, ripple and fluid phases have been successfully detected, by using a fabricated surface micromachined thermal cavity beneath the Pt resistor film.

    DOI: 10.1109/SENSOR.2007.4300261

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  • Active Role of Liposome and Heat Stress on Production and Release of Chitosanase from Streptomyces griceus

    Kien Xuanngo, Huong Thi Bui

    2007   424 - 424   2007

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    DOI: 10.11491/scej.2007.0.424.0

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  • リポソーム固定化電極を用いたタンパク質構造異常性の解析

    石井 治之, 大山 恵奈, 島内 寿徳, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2007   401 - 401   2007

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    DOI: 10.11491/scej.2007.0.401.0

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  • メンブレンチップによる疾病関連タンパク質-生体膜間相互作用の評価

    石井 治之, 大山 恵奈, 島内 寿徳, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2007   690 - 690   2007

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    DOI: 10.11491/scej.2007f.0.690.0

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  • 酸化ストレス負荷SODフラグメント-リポソーム複合体の酵素活性

    馬越 大, Le Quoc Tuan, 濱田 聡, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2007   685 - 685   2007

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    DOI: 10.11491/scej.2007f.0.685.0

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  • 生体膜上におけるAβ/Cu錯体によるカテコール類酸化反応の評価の評価

    島内 寿徳, 松本 匡晴, 石川 大介, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2007   858 - 858   2007

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    DOI: 10.11491/scej.2007f.0.858.0

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  • Aβプロトフィブリルの表面特性の解析と線維形成機構の考察

    島内 寿徳, 西山 圭一, 廣岩 梓, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2007   859 - 859   2007

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    DOI: 10.11491/scej.2007f.0.859.0

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  • メンブレン・ストレスバイオテクノロジーによる分離技術の創成

    久保井 亮一, 馬越 大, 島内 寿徳

    化学工学会 研究発表講演要旨集   2007   686 - 686   2007

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    DOI: 10.11491/scej.2007f.0.686.0

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  • 抗酸化One-Pot反応を触媒するSOD/Catalase-Inspired LIPOzymeの開発

    馬越 大, 森本 研吾, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2007   856 - 856   2007

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    DOI: 10.11491/scej.2007f.0.856.0

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  • in vitro遺伝子発現系におけるストレス負荷生体膜の役割

    馬越 大, Huong Thi Bui, 西田 真人, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2007   857 - 857   2007

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    DOI: 10.11491/scej.2007f.0.857.0

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  • Fluorescence study on the domain formation of N-dodecanoyl-L-tryptophan within a liposome membrane

    Kazuma Yasuhara, Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

    COLLOID AND POLYMER SCIENCE   285 ( 2 )   239 - 243   2006.11

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    The aggregation behavior of N-dodecanoyl-L-tryptophan within a liposome membrane was investigated by fluorescence spectroscopy. Liposomes with a mean diameter of 100 nm, formed from either 1-2-dilauroyl-sn-glycero3-phosphocholine, 1-2-myristoyl-sn-glycero-3-phosphocholine, or 1-2-palmitoyl-sn-glycero-3-phosphocholine, were used. Fluorescence measurements indicate that the sodium salt of N-dodecanoyl-L-tryptophan forms domains with the liposome membranes below the main phase transition temperature, T-m. Above T-m, the molecules are homogeneously dispersed in the liquid crystalline state of the membranes.

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  • Immobilization of liposomes onto quartz crystal microbalance to detect interaction between liposomes and proteins

    S Morita, M Nukui, R Kuboi

    JOURNAL OF COLLOID AND INTERFACE SCIENCE   298 ( 2 )   672 - 678   2006.6

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    To study the interaction between liposomes and proteins, intact liposomes were immobilized on a metal planar support by chemical binding and/or bioaffinity using a quartz crystal microbalance (QCM). A large decrease in the resonance frequency of quartz crystal was observed when the QCM, modified by a self-assembled monolayer (SAM) of carboxythiol, was added to liposome solutions. The stable chemical immobilization of intact liposomes onto SAM was judged according to the degree with which adsorbed mass depended on the prepared size of liposomes, as well as on the activation time of SAMs when amino-coupling was introduced, where the liposome coverage of electrodes was 69 +/- 8% in optimal conditions. When avidin-biotin binding was used on amino-coupling liposome layers, liposome immobilization finally reached 168% coverage of the electrode surface. Denatured protein was also successfully detected according to the change in the frequency of the liposome-immobilized QCM. The adsorbed mass of denatured carbonic anhydrase from bovine onto immobilized liposomes showed a characteristic peak at a concentration of guanidine hydrochloride that corresponded to a molten globule-like state of the protein, although the mass adsorbed onto deactivated SAM increased monotonously. (c) 2005 Elsevier Inc. All rights reserved.

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  • Evaluation of temperature and guanidine hydrochloride-induced protein-liposome interactions by using immobilized liposome chromatography

    N Yoshimoto, M Yoshimoto, K Yasuhara, T Shimanouchi, H Umakoshi, R Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL   29 ( 3 )   174 - 181   2006.4

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    Hydrophobic interactions between nine model proteins and net-neutral lipid bilayer membranes (liposomes) under stress conditions were quantitatively examined by using immobilized liposome chromatography (ILC). Small or large unilamellar liposomes were composed of 1-palinitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and immobilized in a gel matrix by utilizing covalent coupling between amino-containing lipids and activated gel beads or avidin-biotin biospecific binding. Retardation of bovine carbonic anhydrase (CAB) in ILC was pronounced at particular temperatures (50 and 60 degrees C) where the local hydrophobicity of theses protein molecules becomes sufficiently large. Protein-induced leakage of a hydrophilic dye (calcein) from immobilized liposomes interior was also drastically enhanced at particular temperatures where large retardation was observed. For other proteins examined, similar results were also observed. The specific capacity factor of the proteins characteristic for the ILC and the amount of calcein released from immobilized liposomes were successfully expressed as a function of the product of the local hydrophobicities of proteins and liposomes, regardless of protein species and the type of the stress conditions applied (denaturant and heating). These findings indicate that lipid membranes have an ability to non-specifically recognize local hydrophobicities of proteins to form stress-mediated supramolecular assemblies with proteins, which may have potential applications in bioprocesses such as protein refolding and separation. ILC was thus found to be a very useful method for the quantitative detection of dynarnic protein-liposome interactions triggered by stress conditions. (c) 2005 Elsevier B.V. All rights reserved.

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  • Evaluation of carboxylic acid-induced conformational transitions of beta-lactoglobulin: Comparison of the alcohol effects on beta-lactoglobulin

    BK Lee, T Shimanouchi, H Umakoshi, R Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL   28 ( 1 )   79 - 86   2006.2

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    Conformational transitions of bovine P-lactoglobulin A (beta-LG) induced by carboxylic acid were systematically studied by steady-state tryptophan (Trp) fluorescence. The behavior of beta-LG denaturation depends upon the species and concentration of carboxylic acid, as well as on the pH of solutions. The order of the effectiveness of the respective carboxylic acids was described as follows: MeCOOH < EtCOOH < PrCOOH < ClEtCOOH < TFA < iBuCOOH < nBuCOOH < PFPA. The conformational chan-e of beta-LG through the carboxylic acid-induced transitions of the beta-LG conformation were analyzed assuming a two-state mechanism between unfold and native states in order to obtain the m value, a measure of the dependence of the free energy change on the concentration of carboxylic acid. The m values of various carboxylic acids were compared with those of various alcohols based on the role of each group constituting the carboxylic acid and alcohol molecules, namely, the hydrocarbon group, hydroxyl group, halogen substituents, and the carboxyl group. Among these groups, the hydrophobic hydrocarbon groups and halogen substituents contributed positively to the in value, whereas the hydrophilic carboxyl and hydroxyl group contributed negatively. The present results can therefore be interpreted as a simple correlation based on the accessible surface area (ASA) of each groups of carboxylic acids and alcohols. These results suggest that the conformational transition of the protein due to the addition of carboxylic acids and alcohols can be explained both by hydrophobicity as well as clustering effects of each carboxylic acid and alcohol molecule. (c) 2005 Elsevier B.V. All rights reserved.

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  • Analysis of complex permittivity of liposome for its biochemical dynamics up to 30 GHz range

    Minoru Noda, Toshinori Shimanouchi, Hirosuke Suzuki, Masanori Okuyama, Ryouichi Kuboi

    2006 IEEE MTT-S INTERNATIONAL MICROWAVE SYMPOSIUM DIGEST, VOLS 1-5   1983 - +   2006

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    We have successfully measured the complex permittivity of liposome as very important biochemical species, dependent on frequency up to 30 GHz (millimeter-wave range), for the first time, by an open-ended coaxial probe method improved for measuring a very small amount of liquid solution. We used a probe size as small as 1.2 mm phi, which leads to upper frequency limit usable of 90 GHz. Also found that the method is very suitable for evaluation of series of biochemical solution with a small amount (1-2 ml) in a short period. It is revealed, as a result of membrane dynamics of liposome, that 1)temperature dependency of relaxation time of DPPC liposome in dispersion regions(0.2-30 GHz), is attributed to bulk water, cation and anion, 2)increase in the relaxation time results from dynamics of trapping/bounding of water by cation and anion, and 3)cholesterol addition to liposome gives a new dispersion(around 1 GHz), possible to relate to water molecules that freely move in/on the liposome surface. We think the method is a promising tool for exploring basic biochemistry of liposome and related solution systems.

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  • Liposome Enhanced Secretion of Chitosanase from Streptomyces griseus under Heat Stress Condition

    Kien Xuan Ngo, Huong Thi Bui

    2006   1040 - 1040   2006

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  • Membranomeに基づくAlzheimer症へのアプローチ~Membrane Stress Biotechnologyの応用~

    久保井 亮一, 馬越 大, 島内 寿徳

    化学工学会 研究発表講演要旨集   2006   449 - 449   2006

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  • 生体膜情報の解析手法のためのMembrane Chipの開発

    石井 治之, 島内 寿徳, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2006   989 - 989   2006

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  • Heat-enhanced production of chitosanase from Streptomyces griseus in the presence of liposome

    KX Ngo, H Umakoshi, T Shimanouchi, HS Jung, S Morita, R Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   100 ( 5 )   495 - 501   2005.11

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    The effects of heat stress and liposome treatment on the growth of Streplomyces griseus cells and chitosanase production were investigated on the basis of using the designed strategy of a stress-mediated bioprocess. The effective conditions for increasing the interaction between chitosanase and the 1-palmitoyl-2-oleoyl-3-phosphocholine (POPC) liposome under heat stress condition were determined on the basis of the results of circular dichroism (CD) and dielectric dispersion analysis (DDA). Under these effective conditions, S. griseus cells were cultivated for the effective production of chitosanase. The results obtained from both CD spectra and DDA showed that heat stress enhances the interaction of the POPC liposomes and chitosanase. The strongest interaction between them could be obtained in the specific temperature range of 40-45 degrees C. The enhancement of the target chitosanase production was conducted under heat stress at WC in the presence and absence of the POPC liposomes. The growth rates of S. griseus cells in the cases of heat (41 degrees C) and heat (41 degrees C)/POPC treatments were respectively 1.2 and 1.4 times higher than that obtained under the control condition. In the heat (41 degrees C) and heat (41 degrees C)/POPC treatments, chitosanase activity increased to 1.8 and 2 times, respectively, higher than that obtained under the control condition. Heat stress and the addition of the POPC liposomes could therefore be utilized to induce the potential functions of bacterial cells for the enhancement of the final target production.

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  • Oxidation of cholesterol catalyzed by amyloid beta-peptide (A beta)-Cu complex on lipid membrane

    N Yoshimoto, M Tasaki, T Shimanouchi, H Umakoshi, R Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   100 ( 4 )   455 - 459   2005.10

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    A catalytic reaction of H2O2 production by an amyloid beta-peptide (A beta)-Cu complex with cholesterol incorporated in a liposome was kinetically analyzed. The Michaelis-Menten model was applied to the H2O2 production reaction using cholesterol as the substrate catalyzed by the A beta-Cu complex. The K-m value for the A beta-Cu complex catalytic reaction with cholesterol-containing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes (K-m=0.436 mu M for A beta(1-40); K-m=0.641 mu M for A beta(1-42)) was found to be smaller than that with cholesterol-containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes (K-m=0.585 mu M for A beta(1-40), K-m=0.890 mu M for A beta(1-42)). The results imply that membrane properties could play an important role in the interactions of the A beta-Cu complex with cholesterol in these liposomes. Considering the physical states of the cholesterol/POPC (liquid disordered phase) and cholesterol/DPPC (liquid ordered phase) liposomes in the present reaction conditions, the data obtained suggests that the H2O2-gencrating activity of the A beta-Cu complex, accompanied by oxidation of membrane-incorporated cholesterol, could be effected by the phase of the liposome membranes.

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  • Liposome-assisted activity of superoxide dismutase under oxidative stress

    H Nagami, N Yoshimoto, H Umakoshi, T Shimanouchi, R Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   99 ( 4 )   423 - 428   2005.4

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    A biological membrane is the front line of defense for cells against various environmental stresses such as heat and reactive oxygen species (ROS) and is expected to play an important role in the antioxidant system with antioxidant enzymes, similarly to its chaperone-like function in cooperation with heat shock proteins. The oxidative stress response of superoxide dismutase (SOD), which is known to catalyze the dismutation of O-2(-) to H2O2 was investigated in the presence of artificial membranes, liposomes, in order to obtain fundamental information on the biological ROS scavenging system. SOD lost its activity in the presence of H2O, and was found to have two loops including one which contains an a-helix which presents the substrate O-2(-) to the activity center of SOD (Cu(II)). From circular dichroism analysis of SOD in the presence of H2O2, the contents of the a-helix in SOD were found to decrease in correspondence with the inactivation and conformational change of SOD, suggesting that the conformation of the a-helix loops affects SOD activity. In the presence of liposomes composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), SOD was not inactivated in the presence of H2O2 although the contents of its a-helix structure were decreased. The oxidized SOD was found to interact with the liposome surface under oxidative stress using dielectric dispersion analysis. Based on these results, a possible mechanism of SOD protection against ROS on liposomes was presented.

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  • PBL Education for the 2nd Year Undergraduate Students in Chemical Engineering and Continuous Outcomes Assessment

    KUBOI Ryoichi, SHIMADA Wataru, EGASHIRA Yasuyuki, EBITANI Kohki, KINO-OKA Masahiro, TAKAHASHI Hideaki, SATO Hiroshi, UMAKOSHI Hiroshi, SHIRAISHI Yasuhiro, SHIBA Sadataka, SHIMAUCHI Hisanori

    Journal of JSEE   53, 52-57/, ( 6 )   52 - 57   2005

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    A Project-Based Learning (PBL) program has been examined for the 2nd year students who spontaneously set the project themes. The questionnaires to the same students who had taken the PBL course were carried out just after promotion to the 4th year as well as just before graduation. The results demonstrated the effectiveness of the PBL program and the usefulness of the PBL continuous assessment of the outcomes.

    DOI: 10.4307/jsee.53.6_52

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  • Heat-Induced Recovery of Chitosanase from Streptomyces griseus in the Presence of Liposome

    Kien Xuan Ngo

    2005   350 - 350   2005

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  • Variable SOD-like activity of liposome modified with Mn(II)-porphyrin derivative complex

    H Nagami, H Umakoshi, T Shimanouchi, R Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL   21 ( 3 )   221 - 227   2004.11

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    A novel artificial superoxide dismutase (SOD), which can vary its activity in response to oxidative stress, was developed by using the liposome modified with a metal-functional ligand complex. A lipophilic chloro-(5,10,15,20-tetrakis[1-hexadecylpyridinium-4-yl]-21H,23H-porphyrin) (HPyP) was selected as the functional ligand to modify the liposome surface. The effects of the molar ratio of the HPyP and metal ion in the liposome membrane on two kinds of SOD-like functions, i.e. SOD activity and the antioxidative function against phospholipid composed of liposome was investigated by varying the membrane properties of liposomes. The modification of liposome with HPyP complex resulted in conflicting functions, such as an inhibitive and a promotional function during the induction of the antioxidation function against phospholipid, depending on the molar content of HPyP. The Mn(II)-HPyP complex can efficiently convert O-2(-) to H2O2 and O-2 at low HPyP content, while the efficiency of the SOD activity of Mn(II)-HPyP was significantly reduced due to the cluster formation of Mn(II)-HPyP complex in the liposome at a HPyP content exceeding 1 mol%. It was considered that the cluster formation of the Mn(II)-HPyP complex in the liposome may decrease the reactivity of the active center of the disproportional reaction of Mn(II)-HPyP since the complex was buried into the aggregate of Mn(II)-HPyP itself. (C) 2004 Elsevier B.V. All rights reserved.

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  • Comparison of the extraction behavior of metal ions by metal affinity ligands in an organic-aqueous two-phase system and in a liposome system

    H Nagami, H Umakoshi, T Shimanouchi, R Kuboi, Y Baba

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   11   159 - 165   2004

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    The interaction of various metal ions with simple metal affinity ligands, 3(hexadecylthio)propionic acid (HTP) and 2-(hexadecylthio)benzoic acid (HTB), was investigated in an organic/aqueous two-phase system and in a liposome system. Both ligands exhibited a high silver(I) affinity at low pH in the organic/aqueous two phase system but low selectivity in the extraction of other transition metal ions such as Cu(II), Ni(II), Co(II), Zn(II), Cd(II), and Hg(II) above pH 6. On the other hand, remarkably different behavior for metal adsorption was observed in the liposome system with HTP and HTB. HTB lost the ability to interact with metal ions when modified on the liposome surface. This may be attributed to the formation of a hydrogen bond between the carboxyl group of the HTB and the phosphate group of the phospholipid. The HTP-modified liposome effectively adsorbed metal ions of the zinc family, such as Zn(II) and Cd(II), which could interact with the phosphate group of phospholipid, and also adsorbed those transition metal ions, which could be extracted in the organic/aqueous two-phase system. This may be caused by two functions of the liposome, i.e., a gate function for metal ions at the surface of the HTP-modified liposome and additional stabilization by interaction between Cu(II) and the phosphate group of the phospholipid.

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  • Development of PPy films doped with thiol-SAM-Cu particles for NH3 gas sensing

    T Shimanouchi, S Morita, HS Jung, Y Sakurai, Y Suzuki, R Kuboi

    SENSORS AND MATERIALS   16 ( 5 )   255 - 265   2004

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    We have developed a high-sensitivity polypyrrole (PPy) film with a porous structure using a simple and easy method of doping thiol self-assembled monolayer (SAM)-Cu particles into a PPy film. The thiol-Cu-SAM particles were doped by the adsorption of thioxylenol or decanethiol into the porous PPy film surface, resulting in an improvement in sensitivity of the film to NH3. Owing to the addition of thiol-Cu-SAM particles formed using various 4-tent-butylcalix-6-arene thiol derivatives, the sensitivity correspondingly decreased. In particular, for the case of poly(propylene oxide)-calix-6-arenethiol, a marked decrease in sensitivity was observed. Using this composite nanomaterial, we found that the detection limit for NH3 increased to concentration levels of several ppm. We present a novel preparation method for these sensor films aimed at controlling their porosity and sensitivity.

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  • Detection of protein conformation under stress conditions using liposomes as sensor materials

    R Kuboi, T Shimanouchi, M Yoshimoto, H Umakoshi

    SENSORS AND MATERIALS   16 ( 5 )   241 - 254   2004

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    Calcein release from liposomes was analyzed kinetically in the presence of various proteins under the stress condition. In the case of bovine carbonic anhydrase (CAB), a significant release of calcein was observed at pH 4. The conformational change of CAB from the native to the molten-globule (MG) state was confirmed using an aqueous two-phase partitioning method and immobilized liposome chromatography. The CAB-liposome interaction was maximum at a specific pH (4.0), where the CAB conformation was an MG-like state. The results may show that the hydrophobic interaction between CAB and liposomes enhances the perturbation of the liposome membrane, leading to a significant release of calcein from liposomes. These phenomena depended not only on the characteristics of proteins (local hydrophobicity, LHpr) but also on the dynamic properties of liposomes (membrane fluidity). These results obtained with CAB may be extended to other proteins. The manner of protein-induced calcein release was classified into at least two types. Calcein release due to the addition of proteins having either disulfide bonds or being rich in beta-sheet structure was not observed. On the contrary, a significant release of calcein was observed in the case of a reduced protein with a cleaved disulfide bond and in the case of a protein with relatively a low content of beta-sheet structure. These findings suggest that a liposome containing calcein can be used as an effective sensor element for the detection of proteins having large structural fluctuations.

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  • Novel fabrication of conductive polymers contained micro-array gas sensitive films using a micromanipulation method

    HS Jung, T Shimanouchi, S Morita, R Kuboi

    ELECTROANALYSIS   15 ( 18 )   1453 - 1459   2003.10

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    A novel method for fabricating a micro gas sensor film on an indium tin oxide (ITO) electrode patterned using micromachining technology was developed. A micromanipulation system equipped with a counter electrode (Au; circle divide10 pm) and a microsyringe, which was connected to a microinjection system, was first constructed. With this system, micro gas sensor arrays could be successfully prepared on ITO electrodes. Two kinds of micro gas sensor films were prepared, based on polythiophene (PTh) and poly(3-n-dodecylthiophene) (PD). The response behavior of conventional PTh and micro-PTh films against NH3 at three different operating temperatures (25, 40 and 60degreesC) was investigated by measuring the resistance of the film. With the micro-PTh film, a reversible response was observed against NH3 when measured at 40 and 60degreesC. In addition, the responsive characteristics of the microsensor films against different testing gases were examined at the three operating temperatures. The resistance of the microsensor films of PTh and PD changed considerably, depending on the type of testing gas, allowing these sensor films to be used for the detection of various gases. Furthermore, the microsensor films had a high stability compared with conventional films prepared from the same polymer.

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  • Detection of a heat stress-mediated interaction between protein and phospholipid membrane using dielectric measurement

    Seiichi Morita, Toshinori Shimanouchi, Masashi Sasaki, Hiroshi Umakoshi, Ryoichi Kuboi

    Journal of Bioscience and Bioengineering   95 ( 3 )   252 - 256   2003

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    The dielectric response of lipid bilayer membrane vesicles (liposomes) prepared using either phosphatidylcholine from egg (EPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was analyzed at a frequency range of 0.1 to 100 MHz. A marked dielectric dispersion for EPC and POPC liposome suspensions was observed above 1 MHz. An appropriate analysis of the dielectric dispersion curve was performed using the Cole-Cole equation and the Debye equation and was found to provide a method for the determination of dielectric parameters. Among the dielectric parameters, the characteristic frequency of a second dispersion around 50 MHz varied corresponding with changes in the test conditions. Of particular note is that an anomalous change in the characteristic frequency in the presence of protein corresponded to the degree of hydrophobic interaction between proteins and liposomes. The value of the frequency around 50 MHz, as well as the decrease in permittivity over the frequency range tested, are indicators of the interaction between proteins and liposomes.

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  • Characterization of the surface properties of chitosanase under heat stress condition using aqueous two-phase systems and an immobilized-liposome sensor system

    HS Jung, H Umakoshi, SY Son, T Shimanouchi, R Kuboi

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   10   123 - 132   2003

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    The surface properties of chitosanase from Storeptomyces griseus were characterized under heat stress conditions by using aqueous two-phase systems and by using an immobilized-liposome sensor. The surface net (HFS) and local hydrophobicities (LH), were first characterized by using the poly (ethylene glycol) (PEG)/dextran 100-200k (Dex) aqueous two-phase system at various temperatures (25similar to50degreesC). The LH values of dithiothreitol (DTT)-treated chitosanase showed a maximum at a specific temperature (45degreesC) while the HFS values gradually increased with increasing temperature from 40 to 50degreesC. At the same temperature conditions as above, the surface properties of chitosanase were also analyzed by using a sensor tip immobilized-liposome electrode. Similarly in the case of the variation of LH but not HFS, the detected signal (maxima in electric current) was maximized at 45degreesC, and a linear correlation between the LH values and the signal values of the new immobilized-liposome sensor system was obtained. It was thus found that the new sensor system was effective for the characterization of the surface properties and LH values, of proteins, as well as the aqueous two-phase system method.

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  • 誘電分散解析によるタンパク質-脂質膜間相互作用の評価

    佐々木 勝司, 島内 寿徳, 宮川 和也, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2003   1046 - 1046   2003

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  • Phospholipase C誘導型リポソーム膜融合を利用した薬物送達システムの設計

    島内 寿徳, 川崎 英典, 馬越 大, 久保井 亮一

    化学工学会 研究発表講演要旨集   2003   34 - 34   2003

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  • アミロイド性タンパク質の構造転移に及ぼす脂肪酸修飾リポソームの共存効果

    李 奉局, 馬越 大, 島内 寿徳, 久保井 亮一

    化学工学会 研究発表講演要旨集   2003   624 - 624   2003

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  • Characterization and control of stimuli-induced membrane fusion of liposomes in the presence of proteins and stimuli responsive polymers

    MM Felix, H Umakoshi, T Shimanouchi, M Yoshimoto, R Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL   12 ( 1 )   7 - 19   2002.10

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    The process of fusion of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes in the presence of stimuli responsive copolymers (poly(N-isopropylacrylaniide-co-methacrylic acid) and poly(N-isopropylacrylamide-co-methacrylic acid-co-octadecylacrylamide)) or proteins ((x-chymotrypsin (alpha-CT), bovine carbonic anhydrase (CAB), and p-galactosidase (p-gal)) was quantitatively characterized under the stimuli by varying pH and temperature. After the liposomes were exposed to the specific pH and heat stimuli in the presence of the stimuli responsive polymers or proteins, the percentage of the fusion was determined by using two kinds of liposomes entrapping cobalt-calcein and EDTA (cobalt-calcein method). In the presence of stimuli responsive copolymers, the percentage of fusion was increased to 20% above the phase transition condition of the copolymer (above 37 degreesC and below pH 5.7). The percentage of fusion in the presence of proteins was varied under the stimuli, depending on the type of proteins. In the case of the alpha-CT, the maximal percentage of fusion was only 8%. The addition of the CAB and p-gal improved the percentage of fusion to 15 and 20%, respectively, by selecting the optimal stimuli conditions. Although some disagreements were observed in the region of strong acidic conditions, it was found that the increase of the percentage of fusion was well corresponding with the increase of the membrane fluidity of the POPC liposome, followed by the increase of the local hydrophobicity of proteins or copolymers under the stimuli. Based on the above results, a possible model for the stimuli-induced fusion of POPC liposome membranes was finally presented. (C) 2002 Elsevier Science B.V. All rights reserved.

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  • Novel array-type gas sensors using conducting polymers, and their performance for gas identification

    Y Sakurai, HS Jung, T Shimanouchi, T Inoguchi, S Morita, R Kuboi, K Natsukawa

    SENSORS AND ACTUATORS B-CHEMICAL   83 ( 1-3 )   270 - 275   2002.3

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    Novel gas sensor devices have been developed using polythiophene (pTh) film and poly(3-n-dodecylthiophene) (pDpTh.) film coated over pTh film. These polymer films were electrochemically deposited and doped by cyclic voltammetry on thin-film electrodes where the isolation gap was formed by a micromachining process. We examined the response characteristics of the conducting polymer films against various sample gases over a range of temperatures of the sensitive layer. The resistance changes of both sensitive layers of pTh and pDpTh were highly dependent on the kind of layer. In particular, pTh film responded to ammonia gas and pDpTh films clearly responded to hydrophobic gases, such as chloroform, methane and ethanol. The response of these films to several gases was analyzed with a pattern recognition (PARC) algorithm. It was found that our simple gas sensor device could discriminate between the gases that were used here. (C) 2002 Elsevier Science B.V. All rights reserved.

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  • Evaluation of interaction between liposome membranes induced by stimuli responsive polymer and protein

    Matundu Menayame Félix, Hirosh Umakoshi, Toshinor Shimanouchi, Makot Yoshimoto, Ryoich Kuboi

    Journal of Bioscience and Bioengineering   93 ( 5 )   498 - 501   2002

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    Immobilized liposome chromatography was utilized as a novel method for the quantitative evaluation of the interaction between liposome membranes. The capacity factors evaluated from the elution profile showed that interaction between 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposome membranes occurred in the presence of a stimuli responsive polymer and protein under specific stimulus conditions. The occurrence of such interaction was supported by experimental results for POPC liposome membrane fusion under corresponding stimuli conditions.

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  • Characterization of stimuli-induced membrane fusion of liposomes

    Matundu Menayame Félix, Toshinori Shimanouchi, Hiroshi Umakoshi, Makoto Yoshimoto, Ryoichi Kuboi

    Kagaku Kogaku Ronbunshu   28 ( 4 )   481 - 484   2002

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    The phenomena of aggregation and fusion of liposome membranes under the stimuli conditions (heat and pH) were shown to be triggered by the conformational change of the stimuli-responsive polymers and proteins. It is found that the percentage of liposome fusion and the liposome size under the specific stimuli condition were well corresponding with the capacity factor obtained from the peak shift of the elution profile on the immobilized liposome chromatography.

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  • Highly sensitive and selective gas sensors using the polythiophen derivatives controlled the temperature Reviewed

    Y Sakurai, HS Jung, T Inoguchi, T Shimanouchi, M Umakoshi, R Kuboi, K Natsukawa

    TRANSDUCERS '01: EUROSENSORS XV, DIGEST OF TECHNICAL PAPERS, VOLS 1 AND 2   1696 - 1699   2001

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    The advanced odor gas sensor devices were developed using the polythiophene derivatives. These polymers have been found to sense gases and vapours by monitoring the change in resistance on exposure of the polymer to the gas sample. These polymer films were electrochemically deposited and doped by cyclic voltammetry on thin-film electrodes where the isolation gap was formed by the micro-machining process. The breakthrough resulted in sensor devices with unique benefits, such as the very high selectivity and the operation possibility at ambient temperatures (steady and nonsteady state).

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  • Immobilized liposome chromatography for refolding and purification of protein

    Makoto Yoshimoto, Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

    Journal of Chromatography B: Biomedical Sciences and Applications   743 ( 1-2 )   93 - 99   2000.6

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    Small unilamellar liposomes were utilized as a kind of aqueous two-phase system and artificial chaperone which specifically recognize protein conformation with fluctuated structure. Liposomes showed highly selective binding ability to conformationally changed proteins treated with various concentrations of guanidinium hydrochloride, as evaluated by immobilized liposome chromatography (ILC). In refolding of proteins, liposomes bound to refolding intermediate of proteins and prevented them from forming intermolecular aggregates. Refolding of bovine carbonic anhydrase, lysozyme and ribonuclease A was significantly improved in the presence of liposomes. Furthermore, by utilizing ILC, refolding of proteins was also successfully and simply carried out with considerable high reactivation yield. © 2000 Elsevier Science B.V.

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  • Stimuli-responsive separation of proteins using immobilized liposome chromatography

    Toshinori Shimanouchi, Seiichi Morita, Hiroshi Umakoshi, Ryoichi Kuboi

    Journal of Chromatography B: Biomedical Sciences and Applications   743 ( 1-2 )   85 - 91   2000.6

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    The possibility of the stimuli-responsive separation of proteins was investigated using immobilized liposome chromatography (ILC) as novel aqueous two-phase systems. The specific capacity factor (k(s)) of β-galactosidase, obtained by analysis of ILC, was varied by changing the pH of the solution and was maximized at the specific pH of 5 (k(s, max) = 5.57). The k(s) values were found to correspond well with their local hydrophobicities, which can be determined by the aqueous two-phase partitioning method. The variation of k(s), therefore, indicates a change in the surface properties of a protein during conformational change under pH stimuli. A similar phenomenon is observed in the case of other proteins (α-glucosidase, k(s, max) = 11.3 at pH 4
    carbonic anhydrase from bovine, k(s, max) = 6.53 at pH 4). The difference in the height and/or the position of the peaks of the k(s)-pH curves of each protein suggests a difference in their pH denaturation in the ILC column. Based on these results, the mutual separation of the above proteins at pH 4 could be successfully performed by selecting their specific capacity factor as a design parameter. © 2000 Elsevier Science B.V.

    DOI: 10.1016/S0378-4347(00)00061-X

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  • Stimuli-responsive separation of proteins using immobilized liposome chromatography Reviewed

    T Shimanouchi, S Morita, H Umakoshi, R Kuboi

    JOURNAL OF CHROMATOGRAPHY B   743 ( 1-2 )   85 - 91   2000.6

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    The possibility of the stimuli-responsive separation of proteins was investigated using immobilized liposome chromatography (ILC) as novel aqueous two-phase systems. The specific capacity factor (k(s)) of beta-galactosidase, obtained by analysis of ILC, was varied by changing the pH of the solution and was maximized at the specific pH of 5 (k(s,max) = 5.57). The k(s) values were found to correspond well with their local hydrophobicities, which can be determined by the aqueous two-phase partitioning method. The variation of k(s), therefore, indicates a change in the surface properties of a protein during conformational change under pH stimuli. A similar phenomenon is observed in the case of other proteins (alpha-glucosidase, k(s,max) = 11.3 at pH 4; carbonic anhydrase from bovine, k(s,max) = 6.53 at pH 4). The difference in the height and/or the position of the peaks of the k(s)-pH curves of each protein suggests a difference in their pH denaturation in the ILC column. Based on these results, the mutual separation of the above proteins at pH 4 could be successfully performed by selecting their specific capacity factor as a design parameter. (C) 2000 Elsevier Science B.V. All rights reserved.

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  • Immobilized liposome chromatography for refolding and purification of protein Reviewed

    M Yoshimoto, T Shimanouchi, H Umakoshi, R Kuboi

    JOURNAL OF CHROMATOGRAPHY B   743 ( 1-2 )   93 - 99   2000.6

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    Small unilamellar liposomes were utilized as a kind of aqueous two-phase system and artificial chaperone which specifically recognize protein conformation with fluctuated structure. Liposomes showed highly selective binding ability to conformationally changed proteins treated with various concentrations of guanidinium hydrochloride, as evaluated by immobilized liposome chromatography (ILC). In refolding of proteins, liposomes bound to refolding intermediate of proteins and prevented them from forming intermolecular aggregates. Refolding of bovine carbonic anhydrase, lysozyme and ribonuclease A was significantly improved in the presence of liposomes. Furthermore, by utilizing ILC, refolding of proteins was also successfully and simply carried out with considerable high reactivation yield. (C) 2000 Elsevier Science B.V. All rights reserved.

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  • Selective separation process of proteins based on the heat stress- induced translocation across phospholipid membranes

    Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi

    Journal of Chromatography B: Biomedical Applications   711 ( 1-2 )   111 - 116   1998.6

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    Heating of several protein solutions at 40-47°C for 5-60 min in the presence of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes induced the translocation of β-galactosidase (β-gal), α-glucosidase (α- glu) and bovine carbonic anhydrase (CAB) from outer to inner aqueous phase across the liposome membrane. The translocated amounts of β-gal at various temperatures were maximized under suitable heating conditions (45°C, 30 min). Those of α-glu and CAB were maximized at 40-45 and 60°C, respectively. Each maximum value could be correlated with the corresponding local hydrophobicity of each protein evaluated by the aqueous two-phase partitioning method. The possibility to apply these heat-induced translocation phenomena to the bioseparation of proteins was successfully demonstrated for the model mixture solution of β-gal, α-glu and CAB.

    DOI: 10.1016/S0378-4347(97)00659-2

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  • Selective separation process of proteins based on the heat stress-induced translocation across phospholipid membranes Reviewed

    H Umakoshi, T Shimanouchi, R Kuboi

    JOURNAL OF CHROMATOGRAPHY B   711 ( 1-2 )   111 - 116   1998.6

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    Heating of several protein solutions at 40-47 degrees C for 5-60 min in the presence of 1-palmitoyl-2-oleoyI-sn-glycero-3-phosphocholine (POPC) liposomes induced the translocation of beta-galactosidase (beta-gal), alpha-glucosidase (alpha-glu) and bovine carbonic anhydrase (CAB) from outer to inner aqueous phase across the liposome membrane. The translocated amounts of P-gal at various temperatures were maximized under suitable heating conditions (45 degrees C, 30 min). Those of alpha-glu and CAB were maximized at 40-45 and 60 degrees C, respectively. Each maximum value could be correlated with the corresponding local hydrophobicity of each protein evaluated by the aqueous two-phase partitioning method. The possibility to apply these heat-induced translocation phenomena to the bioseparation of proteins was successfully demonstrated for the model mixture solution of P-gal, a-glu and CAB. (C) 1998 Elsevier Science B.V. All rights reserved.

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  • Model system for heat-induced translocation of cytoplasmic beta-galactosidase across phospholipid bilayer membrane

    H Umakoshi, M Yoshimoto, T Shimanouchi, R Kuboi, Komasawa, I

    BIOTECHNOLOGY PROGRESS   14 ( 2 )   218 - 226   1998.3

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    The possibility of the translocation of the enzyme across the phospholipid bilayer membrane was investigated by using the liposomes prepared by 1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine (POPC) in which beta-galactosidase (beta-gal) was entrapped. Exposing the POPC liposomes entrapping beta-gal inside to heat treatment (40-50 degrees C, 1-60 min) was found to induce its translocation across the liposome membrane. The translocated activity of beta-gal from inner to outer aqueous phase of liposomes indicated the maximal value when the liposomes entrapping beta-gal were heated at 45 degrees C for 30 min. The gel permeation profiles of the liposomes before and after heat treatment (45 degrees C, 30 min) also supported the translocation of beta-gal across the liposome membrane. The membrane fluidity of liposomes was found to be increased with increasing temperature, so that the hydrophobicity of liposome membrane was also increased. The local hydrophobicity of beta-gal was maximized at the temperature of 40-50 degrees C. The mechanisms of beta-gal translocation have been suggested to be triggered by the enhancement of hydrophobic interaction between the liposome surface and beta-gal molecules. Finally, a minimal scheme of possible mechanism on the heat-induced translocation of beta-gal has been presented on the basis of the hydrophobic interaction between the liposome and the proteins. The experimental data on the heat-induced translocation of beta-gal were well corresponding to those from model calculation.

    DOI: 10.1021/bp9800135

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Books

  • 分離技術のシーズとライセンス技術の実用化

    島内 寿徳( Role: Contributor ,  マイクロキャピラリー型水熱反応分離装置の応用技術)

    分離技術会編  2018.3 

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  • Pharmaceutical Process Chemistry

    Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi( Role: Contributor ,  Chapter 21, 421-441 (Development of LIPOzyme Based on Biomembrane Process Chemistry))

    Wiley VCH  2010 

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  • 進化する有機半導体

    島内 寿徳, 久保井亮一( Role: Contributor ,  7章2節,p422-430 (導電性高分子/人工細胞膜を用いたストレスセンサの開発))

    NTS  2006 

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  • リポソーム応用の新展開 ~人工細胞の開発に向けて~

    久保井 亮一, 島内 寿徳( Role: Contributor ,  2章2節2,p38-45 (セルサイズリポソームの調製), 9章1節,p472-482 (固定化リポソームクロマトグラフィー), 9章2節,p483-493 (固定化リポソームセンサーとタンパク質の構造異常診断))

    NTS  2005 

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  • Giant Vesicles

    Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi( Role: Contributor ,  Chapter 26, p.369-377)

    John Wiley & Sons  2000 

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  • Study on stress mediated behaviour and preparation of giant vesicles

    Giant Vesicles  1999 

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  • Utilization of Heat Stress Mediated Translocation across Phospholipid Membrane for Protein Separation

    ADVANCES IN BIOSEPARATION ENGINEERING 1996-1997  1998 

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MISC

  • Production of a Monomer of a Biomass Plastic from Woody Biomass~Three Chemical Processes Using Water as Key Technology~

    木村幸敬, 島内寿徳

    水環境学会誌   44(A) ( 7 )   2021

  • Role of Dynamic Space in Lipid Membranes for the Reaction Field

    福間早紀, 島内寿徳, 木村幸敬

    膜   45 ( 3 )   2020

  • Analysis of Microscopic Dynamics of Membrane Interfaces Based on Dielectric Measurement

    島内寿徳, 福間早紀, 木村幸敬

    表面と真空   62 ( 4 )   2019

  • 凍結工程制御に基づく凍結乾燥とPAT技術 Invited

    川崎 英典, 島内 寿徳, 木村 幸敬

    分離技術   48 ( 6 )   37 - 46   2018.12

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  • Molecular Recognition/Transformation Based on Membrane Dynamics

    41 ( 5 )   244 - 250   2016.9

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  • ToF‐SIMSを用いた脂質膜上でのアミロイドβ(1‐40)吸着形態の評価

    横山有太, 島内寿徳, 岩井秀夫, 青柳里果

    表面科学学術講演会講演要旨集   35th   12   2015.12

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  • Discrimination of Target Proteins Using Array Sensor with Liposome Encapsulating Fluorescent Molecules

    32   1 - 4   2015.10

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  • 木質バイオマスの亜臨界水抽出と撥水性界面形成への応用 Invited

    島内寿徳, 神庭朋也, Wei Yang, 木村幸敬

    ケミカルエンジニヤリング   59 ( 7 )   66 - 71   2014.9

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  • A “Membranome”―Based Approach toward “Bio‐Inspired Membrane”

    馬越大, 島内寿徳, 菅恵嗣

    膜   37 ( 6 )   264 - 269   2012.11

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    It is important to establish a methodology to design and develop a "Bio-Inspired Membrane", which can be defined as an "Artificial Membrane" inspired by structures and functions observed in "Biomembrane". Several approaches relating to this research area are reviewed based on a hierarchical view about the structures and characteristics of the above membranes. A "Membranome" research utilizing a gel membrane immobilizing liposome membrane at high concentration is herewith introduced as one of several approaches to create the "Bio-Inspired Membrane". Some possible data required for the "Bio-Inspired Membrane" are finally discussed, focusing on the physicochemical property of self-assembly included in a total system.

    DOI: 10.5360/membrane.37.264

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  • Poster Session

    YOSHIMUNE Miki, KUMAKIRI Izumi, UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori

    37 ( 6 )   301 - 303   2012.11

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  • Leakage Current Microsensor Using Liposome Entrapping Electrolyte for Detection of Target Protein

    TAKADA Keisuke, OHARA Yuri, YAMASHITA Kaoru, NODA Minoru, SHIMANOUCHI Toshinori, UMAKOSHI Hiroshi

    2012 ( 8 )   15 - 19   2012.6

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  • 人工細胞膜上におけるアミロイド形成 Invited Reviewed

    島内 寿徳, 北浦 奈知, 馬越 大, 久保井 亮一

    表面科学   33   40 - 46   2012

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  • 生体膜晶析工学の創成に関する基礎工学的研究 Invited

    島内 寿徳

    膜   36   233 - 239   2011

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  • 脂質膜界面特性に基づくベシクル形成制御

    島内 寿徳, 馬越 大, 久保井 亮一, 石井 治之, 吉本 則子

    化学工学シンポジウムシリーズ   81   107 - 114   2010

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  • 生体膜界面を晶析場とするタンパク質のアミロイド形成/多形の制御 Invited

    島内 寿徳, 北浦 奈知, 大西 諒, 馬越 大, 久保井 亮一

    分離技術   40   455 - 462   2010

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  • 2P-1101 Cotrol of polymorphism of amyloid by liposome membranes

    SHIMANOUCHI Toshinori, OHNISHI Ryo, KITAURA Nashi, UMAKOSHI Hiroshi, KUBOI Ryoichi

    22   34 - 34   2010

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  • MN-P26 Development of membrane chip system for study on membrane-protein interaction(Section X Micro/Nano Technology for Analysis and Cell Manipulation)

    Shimanouchi Toshinori, Ishii Haruyuki, Oyama Ena, Shimauchi Naoya, Umakoshi Hiroshi, Kuboi Ryoichi

    Journal of bioscience and bioengineering   108 ( 1 )   S162 - S163   2009.11

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  • BE-P3 Preparation of liposome immobilized membrane module and its application(Section V Biomolecular Engineering and Bioseparation)

    Umakoshi Hiroshi, Sugaya Hiroyuki, Tohtake Yuji, Shimanouchi Toshinori, Kuboi Ryoichi

    Journal of bioscience and bioengineering   108 ( 1 )   S65   2009.11

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  • Leakage Current Microsensor for Detection of Interaction between Electrolyte-entrapping Liposome and Protein

    ARINOBE Yasushi, ASAI Takeshi, YAMASHITA Kaoru, NODA Minoru, SHIMANOUTI Toshinori, UMAKOSHI Horoshi, OKUYAMA Masanori, KUBOI Ryouichi

    電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan   2009 ( 8 )   57 - 60   2009.7

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  • メンブレン・ストレスバイオテクノロジーとアルツハイマー病の診断

    馬越大, 島内寿徳, 久保井亮一

    分離技術   39 ( 2 )   109 - 114   2009.3

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  • LIPOzyme: Basic and Its Possible Application

    2009

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  • Immobilization of Liposome on Solid Surface: A Quartz Microbalance Study

    J. Colloid Surface Interf.   in press (2009)   2009

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  • Negatively-Charged Liposome is Potent Inhibitor of Protein Folding in Post-Translational Process of in vitro Gene Expression

    Biochem. Eng. J.   in press (2009)   2009

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  • 2Fp06 Analysis of Cell Surface Factor involved in Yeast Flocculation

    ASAYAMA Tomohiro, SHIMANOUCHI Toshinori, UMAKOSHI Hiroshi, KUBOI Ryoichi, TACHIBANA Taro, AZUMA Masayuki

    21   78 - 78   2009

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  • 1Ep20 Amyloid fibril formation on lipid membrane and its polymorphism (Part 3)

    Shimanouchi Toshinori, Shimauchi Naoya, Onishi Ryo, Umakoshi Hiroshi, Kuboi Ryoichi

    21   57 - 57   2009

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  • 1Ep19 Monitoring of amyloid fibril formation on lipid membranes with Membrane Chip (Part 2)

    Shimanouchi Toshinori, Huong Thi Vu, Umakoshi Hiroshi, Kuboi Ryoichi

    21   56 - 56   2009

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  • 1Ep18 Characterization of surface property of amyloidgenic proteins : The use of membrane chip analysis (Part 1)

    Shimanouchi Toshinori, Huong Thi Vu, Shimauchi Naoya, Umakoshi Hiroshi, Kuboi Ryoichi

    21   56 - 56   2009

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  • Bio-thermochemical sensor of microbolometer with a small amount of immobilized liposome on Pt surface

    ASAI Takeshi, YAMASHITA Kaoru, NODA Minoru, SHIMANOUCHI Toshinori, OKUYAMA Masanori, KUBOI Ryoichi

    2008 ( 1 )   31 - 34   2008.6

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  • Characterization of Surface Properties of Streptomyces griseus Cell after Heat Treatment with Liposome Using Aqueous Two-Phase Method

    2008

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  • Cutting Edge of Membrane Stress Biotechnology

    KUBOI Ryoichi, UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori

    Membrane   33(6), 300-306 (2008) ( 6 )   300 - 306   2008

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    A "Biomembrane"is a key interface to integrate production and separation of target materials through the creationof "new order"on its surface. A model biomembrane, liposome, can recognize (separate)the (bio)moleculesthrough (i)electrostatic, (ii)hydrophobic interaction, and (iii)stabilization effect of hydrogen bonds in the hydropho-bic lipid environment. A novel biomembrane-based catalysis (LIPOzyme: liposome + enzyme)can be designed anddeveloped by integrating the above recognition sites and a simple catalytic center on the liposome surface. A processdesign utilizing LIPOzyme chemistry and the recognition (bioseparation)functions of model-biomembrane (lipo-some)is the basic strategy to achieve a "Biomembrane Process Chemistry"as an application of "Membranomics".

    DOI: 10.5360/membrane.33.300

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  • Cutting-Edge of Membrane Stress Biotechnology

    KUBOI Ryoichi, UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori

    MEMBRANE   33(6), 300-306 (2008) ( 6 )   300 - 306   2008

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    Language:Japanese   Publisher:THE MEMBRANE SOCIETY OF JAPAN  

    A "Biomembrane"is a key interface to integrate production and separation of target materials through the creationof "new order"on its surface. A model biomembrane, liposome, can recognize (separate)the (bio)moleculesthrough (i)electrostatic, (ii)hydrophobic interaction, and (iii)stabilization effect of hydrogen bonds in the hydropho-bic lipid environment. A novel biomembrane-based catalysis (LIPOzyme: liposome + enzyme)can be designed anddeveloped by integrating the above recognition sites and a simple catalytic center on the liposome surface. A processdesign utilizing LIPOzyme chemistry and the recognition (bioseparation)functions of model-biomembrane (lipo-some)is the basic strategy to achieve a "Biomembrane Process Chemistry"as an application of "Membranomics".

    DOI: 10.5360/membrane.33.300

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  • Immobilized Liposome Chromatography/Bio-Membrane Module for Bioseparation and Bioreactor

    2008

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  • LIPOzyme Design Based on Molecular Recognition Function of Liposome -Biomembrane Process Chemistry and Separation Technology-

    2008

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  • Separation and Analysis for Aβ Peptides Based on Recognition Function of Abnormal Biomembrane ~Membrane Chip Analysis Can Brighten a Potential Aspect of Alzheimer’s Disease~

    2008

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  • Cationic Liposome DOTAP can Knock-out mRNA and Silence Its Translation in E.coli Cell Free Translation System

    2008

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  • Simple Ligand/Porphyrin Complex can Induce Both SOD and POD Activities Similar to Those of Metal-Ligand Complex

    2008

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  • Preparation and Properties of Antioxidative LIPOzyme

    2008

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  • Biomembrane Process Chemistry and Separation Technology~ Design and Development of Antioxidative LIPOzyme in One-Pot Based on Molecular Recognition Function of Liposome

    2008

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  • LIPOzyme design based on molecular recognition function of liposome

    2008

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  • Amyloid fibril formation on biomembrane: Analysis using membrane chip system

    2008

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  • Liposome-Assisted Refolding of Microbial Transglutaminase

    UMAKOSHI Hiroshi, YOSHIMOTO Noriko, YOSHIMOTO Makoto, SHIMANOUCHI Toshinori, KUBOI Ryoichi

    Membrane   32 ( 5 )   287 - 293   2007.9

  • Immobilized Liosome Sensor and Its Application to Membrane-Related Diseases

    KUBOI Ryoichi, UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori

    MEMBRANE   32(1), 32-39 ( 1 )   32 - 39   2007

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    The diseases caused by the membrane disorder or damages are categorized as the Membrane-related diseases. Alzheimer's disease (AD), which is one of the membrane-related diseases, has been considered to be accompanied by the oxidative damage of the neuronal cell membrane. Amyloid β-peptide (Aβ), which is a pathological key peptide of AD, interacts with biomembrane and this interaction has been recently paid attention from the viewpoints of not only conformational abnormality but also the influence to biomembrane. And therefore, the modeling of biomembrane using liposome has been desired to develop the liposome-based sensor system. In this article, we surveyed the sensor system using liposome. Firstly, the immobilization techniques for preparation of the immobilized-liposome sensor are surveyed. Secondary, some kinds of sensor have been introduced: (i) (metal affinity) immobilized-liposome chromatography, (ii) immobilized-liposome quartz crystal microbalance method, (iii) immobilized-liposome sensor (Amperometric method), (iv) membrane chip system. These techniques have been developed in a framework of "Membrane Stress Biotechnology". The above methodologies could provide us the quantitative properties of stress responsive function of both the protein and the membrane.

    DOI: 10.5360/membrane.32.32

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  • Immobilized Liposome Sensor and Its Application to Membrane-Related Diseases Invited

    KUBOI Ryoichi, UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori

    MEMBRANE   32 ( 1 )   32 - 39   2007

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:THE MEMBRANE SOCIETY OF JAPAN  

    The diseases caused by the membrane disorder or damages are categorized as the Membrane-related diseases. Alzheimer's disease (AD), which is one of the membrane-related diseases, has been considered to be accompanied by the oxidative damage of the neuronal cell membrane. Amyloid β-peptide (Aβ), which is a pathological key peptide of AD, interacts with biomembrane and this interaction has been recently paid attention from the viewpoints of not only conformational abnormality but also the influence to biomembrane. And therefore, the modeling of biomembrane using liposome has been desired to develop the liposome-based sensor system. In this article, we surveyed the sensor system using liposome. Firstly, the immobilization techniques for preparation of the immobilized-liposome sensor are surveyed. Secondary, some kinds of sensor have been introduced: (i) (metal affinity) immobilized-liposome chromatography, (ii) immobilized-liposome quartz crystal microbalance method, (iii) immobilized-liposome sensor (Amperometric method), (iv) membrane chip system. These techniques have been developed in a framework of "Membrane Stress Biotechnology". The above methodologies could provide us the quantitative properties of stress responsive function of both the protein and the membrane.

    DOI: 10.5360/membrane.32.32

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  • DESIGN AND DEVELOPMENT OF MEMBRANE CHIP SYSTEM FOR STRESS SENSOR

    Umakoshi Hiroshi, Shimanouchi Toshinori, Kuboi Ryoichi

    Annual Report of FY 2006, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST)   21 - 24   2007

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  • 3C10-4 Effect of Hydrophobicity of Abeta Oligomer Cross-Linked by Transglutaminase on Amyloid Fibril Formation

    UMAKOSHI Hiroshi, HAMADA Satoshi, SHIMANOUCHI Toshinori, KUBOI Ryoichi

    19   90 - 90   2007

  • 3C10-5 Roles of Liposomes and Heat Stress for Enhanced Release of Chitosanase from Streptomyces griseus :

    NGO Kien Xuan, UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori, KUBOI Ryoichi

    19   91 - 91   2007

  • 1B11-1 Analysis of amyloid fibril formation based on the surface property of protofibrils

    SHIMANOUCHI Toshinori, HIROIWA Azusa, NISHIYAMA Keiichi, UMAKOSHI Hiroshi, KUBOI Ryoichi

    19   59 - 59   2007

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  • DEVELOPMENT OF ELECTROACTIVE POLYMETHYLTHIOPHENE BASED DOPAMINE SENSOR

    Vu Thi Huong, Kuboi Ryoichi, Pham Hung Viet, Shimanouchi Toshinori, Nguyen Minh Tue, Do Phuc Quan

    Annual Report of FY 2006, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST)   41 - 46   2007

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  • DESIGN AND DEVELOPMENT OF OXIDATIVE STRESS RESPONSIVE LIPOSOME MEMBRANE WITH ENZYMATIC ACTIVITY (LIPOZYME) AND ITS APPLICATION TO CHEMICAL/BIOSENSOR

    Kuboi Ryoichi, Shimanouchi Toshinori, Umakoshi Hiroshi, Le Quoc Tuan

    Annual Report of FY 2006, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST)   63 - 66   2007

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  • LIPOzyme: design and development of artificial chaperone/enzyme liposome

    2006

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  • Design of stress-mediated bioprocess using liposome - recovery of chitosanase from Streptomyces griseus cells

    2006

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  • Study on Interaction of Amyloid b-Peptide with Liposome

    2006

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  • Stress-mediated preparation of liposomes incorporating membrane protein

    2006

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  • Membrane Chip: Dynamic Analysis of Stressed Membrane-Membrane Interaction

    2006

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  • Membrane Stress Biotechnology

    2006

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  • Design of Stress Sensor Using Conducting Polymers/Artificial Cell Membranes

    7章2節   2006

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  • Use LIPOzyme for Stress-Mediated Bioreactor and Bioseparation

    2006

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  • Design of Stress Sensor Using Conducting Polymers/Artificial Cell Membranes

    7章2節   2006

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  • 1F12-1 SOD Activity with Support of Phospholipid Membrane under Oxidative Stress :

    TUAN Le quoc, NAGAMI Hideto, UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori, KUBOI Ryoichi

    18   94 - 94   2006

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  • 2F11-4 Metaloenzymatic function of Amyloid beta-metal complex on liposome membrane : cholesterol oxidation

    TASAKI Makoto, YOSHIMOTO Noriko, SHIMANOUCHI Toshinori, UMAKOSHI Hiroshi, KUBOI Ryoichi

    18   102 - 102   2006

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  • 2F09-4 Study on solubilizaiton of amyloid fibril by oxidative derivatives of dopamine

    SHIMANOUCHI Toshinori, ISHIKAWA Daisuke, NOZAWA Atsushi, UMAKOSHI Hiroshi, KUBOI Ryoichi

    18   99 - 99   2006

  • 2G09-4 Evaluation of SOD-like activity of artificial enzymatice liposome modified with multi-nuclear metal

    OHAMA Yuji, TUAN Le Quoc, SHIMANOUCHI Toshinori, UMAKOSHI Hiroshi, KUBOI Ryoichi

    18   120 - 120   2006

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  • Development of Chemical-and Bio-sensor for Environmental Monitoring

    Shimanouchi Toshinori, Kuboi Ryoichi

    Annual Report of FY 2005, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST)   41 - 46   2006

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  • Immobilized Liposome Chromatography

    472-482   2005

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  • Preparation Method of Cell-Sized Liposomes

    38-45   2005

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  • Immobilized Liposome Chromatography

    472-482   2005

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  • Metal-affinity immobilized liposome chromatography to detect conformational change of peptide

    2005

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  • Heat enhanced recovery of chitosanase from Streptomyces griceus using aqueous two-phase system

    2005

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  • Detection of Conformational Change of Protein Based on Protein-Liposome Interaction

    2005

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  • Metal affinity-immobilized liposome chromatography for detection of conformational change of peptide

    2005

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  • タンパク質の構造異常疾患におけるアミロイド線維の制御 〜メンブレン・ストレスバイオテクノロジーの視点から〜 Invited

    島内 寿徳, 馬越 大, 久保井 亮一

    分離技術   35 ( 6 )   391 - 396   2005

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  • Preparation Method of Cell-Sized Liposomes

    38-45   2005

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  • DESIGN AND DEVELOPMENT OF NANO-ARTIFICIAL-CELL MEMBRANE BASED NOVEL BIOSENSOR : APPLICATION FOR MONITORING OF AQUEOUS STRESSES

    Kuboi Ryoichi, Morita Seiichi, Yoshimoto Makoto, Ho-Sup Jung, Shimanouchi Toshinori, Umakoshi Hiroshi

    Annual Report of FY 2004, The Core University Program between Japan Society for the Promotion of Science (JSPS) and Vietnamese Academy of Science and Technology (VAST)   13 - 16   2005

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  • Detection of Protein Conformation under Stress Condition Using Liposome as Sensor Materials

    2004

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  • メンブレン・ストレスバイオテクノロジー 〜薬物送達システムの設計と開発への応用〜

    2004

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  • Detection of Protein Conformation under Stress Condition Using Liposome as Sensor Materials

    2004

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  • メンブレン・ストレスバイオテクノロジー ~薬物送達システムの設計と開発への応用~

    2004

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  • Metal Affinity-Immobilized Liposome Chromatography for Stress Mediated Separation of Biomolecules

    2004

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  • Different Roles of Metal Ions Both in the Formation of Amyloid b-Fibri and Superoxide

    2004

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  • Characterization of Stress-Response Behavior of SOD/CAT on Liposome under Heat/Oxidative Stresses

    2004

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  • メンブレン・ストレス応答ダイナミクスのモニタリング技術の開発 Invited

    島内 寿徳, 森田 誠一, 久保井 亮一

    生物工学会誌   82 ( 5 )   208 - 211   2004

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:日本生物工学会  

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    Other Link: http://dl.ndl.go.jp/info:ndljp/pid/10514808

  • 3I10-2 Coexistence effect of fatty acid-modified liposome on amyloid under stress condition

    LEE Bong-Kuk, NISHIMURA Daisuke, UMAKOSHI Hiroshi, SHIMANOCUHI Toshinori, KUBOI Ryoichi

    16   229 - 229   2004

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  • 3I10-1 Design and development of liposomal membrane : Application for monitoring of aqueous stressors

    SASAKI Masashi, LEE Bon-Kuk, SHIMANOUCHI Toshinori, MORITA Seiichi, KUBOI Ryoichi

    16   229 - 229   2004

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  • Characterization of the surface properties of chitosanase under heat stress condition using aqueous two-phase systems and an immobilized-liposome sensor system

    HS Jung, H Umakoshi, SY Son, T Shimanouchi, R Kuboi

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN   10 ( 10 )   123 - 132   2003

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    The surface properties of chitosanase from Storeptomyces griseus were characterized under heat stress conditions by using aqueous two-phase systems and by using an immobilized-liposome sensor. The surface net (HFS) and local hydrophobicities (LH), were first characterized by using the poly (ethylene glycol) (PEG)/dextran 100-200k (Dex) aqueous two-phase system at various temperatures (25similar to50degreesC). The LH values of dithiothreitol (DTT)-treated chitosanase showed a maximum at a specific temperature (45degreesC) while the HFS values gradually increased with increasing temperature from 40 to 50degreesC. At the same temperature conditions as above, the surface properties of chitosanase were also analyzed by using a sensor tip immobilized-liposome electrode. Similarly in the case of the variation of LH but not HFS, the detected signal (maxima in electric current) was maximized at 45degreesC, and a linear correlation between the LH values and the signal values of the new immobilized-liposome sensor system was obtained. It was thus found that the new sensor system was effective for the characterization of the surface properties and LH values, of proteins, as well as the aqueous two-phase system method.

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  • 固定化リポリームクロストグラフィーによる生理活性物質の分離・分析 Invited Reviewed

    ぶんせき   11   636 - 642   2003

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  • Development of Liposomal Artificial SOD controlled by Oxidative Stress

    Nagami Hideto, Kitaura Takenori, Shimanouchi Toshinori, Umakoshi Hiroshi, Kuboi Ryoichi

    15   215 - 215   2003

  • 医薬品製造における晶析技術 タンパク質の構造異常化によるストレス誘導型アミロイドーシス

    島内寿徳, 馬越大, 久保井亮一

    分離技術   32 ( 6 )   347 - 351   2002.11

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  • Eraluation of Interaction between Liposome Membranes Induced by Stimuli Reponsire Polymers and Proteins

    Journal of Bioscience and Bioengineering   93(5), 498-501/,   2002

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  • ストレス応答型バイオプロセスのためのストレスセンサの開発 Invited

    鄭 浩燮, 島内 寿徳, 馬越 大, 久保井 亮一, 金 廣, 櫻井 芳昭, 夏川 一輝

    ケミカルエンジニヤリング   47   752 - 755   2002

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  • タンパク質の構造異常・アミロイドーシスとそのセンシング Invited

    島内 寿徳, 佐々木 勝司, 久保井 亮一

    ケミカルエンジニヤリング   47   748 - 751   2002

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  • Selective separation process of proteins based on the heat stress-induced translocation across phospholipid membranes

    UMAKOSHI Hiroshi, SHIMANOUCHI Toshinori, KUBOI Ryoichi

    J. Chromatogr. B.   711 ( 1 )   111 - 116   1998.6

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Awards

  • 研究助成

    2020.4   八雲環境科学研究財団   環境低負荷な還元剤フリー金属ナノ粒子 形成法とその応用

    島内 寿徳

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  • 研究助成

    2016.8   八雲環境科学研究財団   金属無機塩と脂質膜からなるハイブリッド触媒による環境低負荷型反応プロセスの開発

    島内 寿徳

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  • 岡山工学振興会科学技術賞

    2015.7   岡山工学振興会   水晶振動子を活用するアルツハイマー病治療技術への展開

    島内 寿徳

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  • 研究助成

    2012.12   大川情報通信基金   生体膜異常化の解析アルゴリズムの構築

    島内 寿徳

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  • Young Scientist Award of Osaka University

    2011.11   Osaka University  

    SHIMANOUCHI Toshinori

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  • Young Scientist Award of The Membrane Society of Japan

    2011.5  

    SHIMANOUCHI Toshinori

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  • 第六回 大阪大学論文百選 (2005年度)

    2006  

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  • 6th Annual Report 2005-2006

    2006  

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  • 第五回 大阪大学論文百選 (2004年度)

    2005  

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  • 第五回 大阪大学論文百選 (2004年度)

    2005  

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  • Outstanding Paper Award in APCChE2004 (SCEJ) (Oct.,2004, Kita-Kyushu)

    2004  

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  • Outstanding Paper Award in APCChE2004 (SCEJ) (Oct.,2004, Kita-Kyushu)

    2004  

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  • Outstanding Paper Award in APCChE2004 (SCEJ) (Oct.,2004, Kita-Kyushu)

    2004  

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  • Outstanding Paper Award in APCChE2004 (SCEJ) (Oct.,2004, Kita-Kyushu)

    2004  

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Research Projects

  • Development of new bioseparation material aiming at both suppression of surface contamination and repair of biomolecules

    Grant number:21H01692  2021.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    島内 寿徳

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    Grant amount:\17290000 ( Direct expense: \13300000 、 Indirect expense:\3990000 )

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  • Fundamental study on bioelectronic chip for diagnosis of pathologic conditions based on specific and ultrahighly sensitive detection of prion-like protein

    Grant number:20H00663  2020.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    野田 実, 山門 穂高, 澤村 正典, 島内 寿徳, 寒川 雅之, 福澤 理行

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    Grant amount:\44980000 ( Direct expense: \34600000 、 Indirect expense:\10380000 )

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  • マイクロ流体システムによるアミロイドーシス指向型バイオ機能性ナノカプセルの設計

    2020.04 - 2022.03

    日本学術振興会  二国間交流事業  共同研究(日本-韓国)

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  • A construction of new biosensor technology enabling highly sensitive detection of alpha-synuclein fibril originated from Parkinson's disease patient

    Grant number:19K22964  2019.06 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)  Grant-in-Aid for Challenging Research (Exploratory)

    野田 実, 山門 穂高, 島内 寿徳, 寒川 雅之

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    Grant amount:\6370000 ( Direct expense: \4900000 、 Indirect expense:\1470000 )

    本研究では、申請者が研究、開発してきたリポソーム固定化カンチレバーセンサ技術とRT-QuIC法という異常凝集タンパク質(aSyn)増幅技術を組み合わせて、aSyn凝集体の特異的かつ超高感度な検出の可能性を探索することを目的とする。今まで申請者はaSynフィブリル自体は10 nMオーダーまで高感度検出できているが、臨床医学では数十fMレベルの感度での特異的検出が必要である。臨床医学での現使用技術では感度自体、特に再現性の点で能力不足で実用レベル技術は現在無い。
    本現況に対して本年度実施した研究では新たなバイオプロトコルとして、「その微量フィブリルのモノマーへの自己鋳型化現象」がリポソーム等の本分子系で発現するかを検討した。比較的高濃度であるμMオーダーのaSynモノマー溶液に極微量、例えば百fMオーダーのフィブリルを添加した場合、自己鋳型化による増幅現象を本センサで検出できるか実験、確認した。その結果、約700fMのフィブリルを数時間で検出できた。この結果は非常に画期的であり、臨床医学側からのパーキンソン病患者、患者予備軍の方々から生体液、主に髄液中で、他のタンパク質等バイオ分子夾雑物からのaSynフィブリルを特異的に検出する次のステージに進めることができた。
    一方本研究を構成する基礎技術の研究成果として、脂質技術として従来のゲル相と異なり液晶相DOPCを用いた結果、高いaSyn検出感度を得た。また糖脂質C16, C18修飾で検出感度増大を確認し糖脂質の相分離によるaSyn-糖脂質間相互作用が多点結合と推定できた。基盤センサ技術となるカンチレバーセンサで応力検出の根幹となる応力集中箇所にひずみ抵抗を形成、設計、試作した結果、荷重印加によるひずみ抵抗変化で従来の20倍程度の感度向上を達成することができた。このセンサ基礎性能の向上により上記からさらなる高感度検出が可能となった。

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  • Mathematical analysis of the characteristic properties of nano-machines in a blood vessel

    Grant number:16KT0133  2016.07 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Obuse Kiori

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    Grant amount:\4810000 ( Direct expense: \3700000 、 Indirect expense:\1110000 )

    We have performed numerical simulations of the dynamics of nanomachines in 3D Navier-Stokes flow in blood-vessel-like tube. It was suggested that the ratio of the representative length of nanomachines and the width of the holes on the boundary of the blood-vessel-like tube is important to control the probability of nanomachines’reaching the target cells outside the tube.
    We have also considered simple mathematical models to discuss the above situation. The dynamics of a nanoparticle in 2D Stokes shear flow in a semi‐infinite plane with a hole and in a 2D tube with boundaries where nonslip and slip boundary conditions are periodically considered were investigated by utilising complex analysis and numerical calculations. Here again, it was suggested that the ratio of the particle radius and the width of the hole/interval where slip boundary condition is considered are important to control the dynamics of the nanoparticle.

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  • Fabrication and development of biosensor to detect aggregation of amyloid-beta oligomer

    Grant number:16K14254  2016.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    Noda Minoru

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    Grant amount:\3640000 ( Direct expense: \2800000 、 Indirect expense:\840000 )

    Liposome biosensors are developed to detect dynamics of aggregation and fibrillization of amyloid-beta oligomer. Those sensors are expected to realize early and simple diagnosis and evaluation of progress in pathological change of Alzheimer Disease that expresses aggregation and accumulation of abnormal protein. In this research, both sensor device and biosensing phospholipid of liposome are investigated and developed as follows: I. Construction of the sensor device, II. Improvement in sensitivity of the sensor, and III. Comparison with ELISA result. A micro-TAS chip comprising of liposome cantilever sensor and microfluidic channels was fabricated. Consequently, dynamic aggregation and fibrillization of amyloid-beta related to its oligomer was selectively detected in human serum. Also, a high sensitivity was estimated that can detect less than 1 nM by excellent selection of phospholipid species, techniques of low-noise electronic circuit and precise temperature control of substrate.

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  • Membrane-on-Membrane ~Creation of Hierarchical Self-Organizing Materials~

    Grant number:26249116  2014.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    Umakoshi Hiroshi, Ishigami Takaaki, Iwasaki Fumihiko, Hirose Masanori, Taguchi Shogo, Watanabe Nozomi

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    Grant amount:\40170000 ( Direct expense: \30900000 、 Indirect expense:\9270000 )

    In this project, a novel membranous devise (material) was developed. Membrane-on-Membrane (MoM) was hierarchically composed by self-assembled membranes. Liposome was selected as core unit of MoM, because it showed unique functions such as recognition of L-amino acid, promoting organic reaction in water, and molecular orientation of chlorophyll. In addition, the methodologies, multiple-fluorescent probe analyses, membrane surface-enhanced Raman spectroscopy, dielectric dispersion (relaxation) spectroscopy, were developed to characterize the physicochemical properties of membranes (in micro- and mesoscale). As an example of our achievements, the “ordered” membrane property plays a crucial role to improve selectivity in chiral recognition of amino acid. When the liposome possessing ordered membrane property was applied to MoM device (for example, liposome-immobilized polyacrylamide hydrogel system), the chiral recognition ability could be maintained.

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  • Liposome Physical Sensors Oriented to Label-free Integrated Chip for Early Diagnosis of Diseases Originated from Amyloid-beta Abnormality

    Grant number:26630157  2014.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    Minoru Noda, SHIMANOUCHI Toshinori, MATSUOKA Teruyuki, YAMASHITA Kaoru, UMAKOSHI Hiroshi, NARIMOTO Jin

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    Grant amount:\4030000 ( Direct expense: \3100000 、 Indirect expense:\930000 )

    We have developed a new biosensing system composed of arrayed cell structure for dielectric dispersion analysis (DDA) of interaction with phospholipid membrane of liposome in order to enable to use plural different phospholipids as sensing molecule, as similar to cell membrane, and to measure simultaneously plural different target biomolecules such as proteins with different conditions. When measuring with normal human serum, we have successfully observed phenomena relating to fibrillization and/or aggregation of Aβ(1-40) with interaction of the liposomes by the DDA, also confirmed by a different sensing of cantilever sensor. Finally, we have detected 1 microM Aβ(1-40) by the DDA method, implying that the concentration in Alzheimer disease patient (20-500 nM) could be measured by an order of improvement.

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  • Desgin of Biointerfaces with Protein Refolding Property, Based on Amyloid Fibril Formation

    Grant number:24686086  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)  Grant-in-Aid for Young Scientists (A)

    SHIMANOUCHI Toshinori

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    Grant amount:\27170000 ( Direct expense: \20900000 、 Indirect expense:\6270000 )

    In this study, we developed the noveland medical bio-interfaces with protein refolding property, based on the amyloid fibril formation. We used the variety of phospholipids, detergents, and polymers to investigate their kinetic interfacial properties such as fluidity and interfacial energy. Furthermore, the kinetic parameters including the nucleation time and elongation rate constant for the fibril formation were evaluated and its mechanistic detailes was discussed, taking into consideration of influence of the prepared interfaces. Consequently, it was suggested that the constituent of interfaces should require the molecular fluidity within the interface enough to allow the orientation of proteins into the interior of interfaces.

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  • Developmentofcontrol for amyloid toxicity by novel Screening system

    Grant number:23656525  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    SHIMANOUCHI Toshinori

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    Grant amount:\3770000 ( Direct expense: \2900000 、 Indirect expense:\870000 )

    Amyloid fibrils is generally propagated on biomembranes to induce the cell toxicity. Meanwhile, there is no methodology to stop the aforesaid event. In this study, we challenged the development of toxicity control of amyloid fibrils using liposomes. It has been demonstrated that liposome library could give (i) the adequate liposome with lipid composition advantageous for a control of toxicity of amyloid fibrils and (ii) LIPOzyme with the function to promote the inhibitory of fibril formation or disaggregatiojn of fibrils. Those results have implied a possibility on the development of detoxic process of amyloid fibrils by using liposomes.

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  • 光学分割LIPOzymeを用いたキラル分離膜モジュールの開発

    Grant number:22656177  2010 - 2012

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    馬越 大, 久保井 亮一, 島内 寿徳

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    Grant amount:\2170000 ( Direct expense: \1900000 、 Indirect expense:\270000 )

    キラル分離法あるいは(化学的処理酵素的処理を伴う)不斉合成法は,医薬品製造プロセスの経済性・操作性・環境負荷を決定付ける重要なキー単位操作である.光学異性体を高度分離するために,キラル分離ならびに不斉合成のコア材料として,リボソーム,ならびに,不斉合成LIPOzymeを安定に固定化した膜モジュールを調製する.網羅的な解析データに基づいて,キラル認識の原理に迫り,同時に,大規模分離に耐えられる分離膜としての可能性を探る.初年度では,リボソームのキラル分離データベース:リボソームのキラル分離能力の系統的解析により基礎データベースを拡充することを目的とした
    まず,自然界に存在するL体の脂質から成るリボソーム((L)-リボソーム,と表記)を用いて,L体ならびにD体のアミノ酸のキラル分離能力を検討した.その結果,L-アミノ酸は(L)-リボソームに吸着したが,D-アミノ酸は吸着しなかった.特に,L-トリプトファンは97%吸着したが,D-トリプトファンは0%であった.さらに,L-/D-トリプトファン等モル混合溶液(ラセミ体)の光学分割にも成功した.アミノ酸吸着機構を検討した結果,静電的/疎水的相互作用,水素結合,ならびに不正炭素の配置が鍵であることが示唆された。また,吸着アミノ酸は脂質膜表面を流動化すると脱離することが確認され,分離プロセスとしての応用も可能であることが分かった.本萌芽技術は特許出願している
    2年目以降,不斉合成LIPOzyme : LIPOzyme触媒との組合せによるエナンチオ選択的な化学変換(酸化・還元・加水分解ほか)手法に関する基礎的なデータを集積する.さらに,3年目では,光学分割LIPOzyme膜モジュール:既存の独自技術であるリボソーム固定化膜モジュールを用いて,モジュール内部に上記LIPOzymeを固定化し,モジュール性能を検証する予定である

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  • Studies on development and stabilization of intact liposome biosensor technology for Si-integrated structure

    Grant number:22360144  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    NODA Minoru, SHIMANOUCHI Toshinori, YAMASHITA Kaoru, FUKUZAWA Masayuki, SOHGAWA Masayuki, KUBOI Ryouich, UMAKOSHI Hiroshi, OKUYAMA Masanori

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    Grant amount:\18980000 ( Direct expense: \14600000 、 Indirect expense:\4380000 )

    In order to improve and stabilize the precision and performance of liposome biosensor and its sensing technologies, a series of technical considerations and resultant improvements have been done on the sensor as a key component of near future Si-integrated microsensor system. The technical developments are started from immobilization of minute droplet of liposome suspension and intact supramolecule structure. Moreover, new biosensing systems of liposome impedance biosensor and fluorescence array sensor are developed, based on electronics techniques. Finally, the sensitivities and performance of both leakage current sensor and cantilever strain sensor are improved because a resultant quantitative capability of immobilization of minute liposome droplet on the sensor becomes improved. We also note that the impedance sensor can detect the interaction between the liposome and target protein with a 100 nL order. And that the fluorescence array sensor system can discriminate different target biomolecules.

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  • Study on Peptide Display on Liposome Membranes for Disaggregation of Amyloid Fibrils

    Grant number:21246121  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    KUBOI Ryoichi, SHIMANOUCHI Toshinori, HIROSHI Umakoshi, YOSHIMOTO Makoto, SHIOMORI Kouichiro, GOTO Yuji

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    Grant amount:\45370000 ( Direct expense: \34900000 、 Indirect expense:\10470000 )

    We systematically investigated the polymorphism of amyloid Aβ/ Cu fibrils and their catalytic oxidation activity using the liposome as a model biomembrane. It was clarified that the oxidized surface of liposome could affect the catalytic oxidation activity of amyloids as well as their morphology. It was revealed that the experiments using peptide fragments-displayed liposomes could contribute to the better understanding on the regulatory mechanism of catalytic oxidation activity of Aβ/ Cu. Besides, we developed the novel loading method of liposomes into the module. Those results suggested that a recognition system of abnormal/ damaged biomembranes for their repairmen could be in principle developed, from those results.

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  • Densign and Development of Stress Responsive LIPOzyme Reactor Based on Re-functionalization of Junk Peptides

    Grant number:20360350  2008 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    UMAKOSHI Hiroshi, KUBOI Ryoichi, SHIMANOUCHI Toshinori, SHIOMORI Koichiro, NAKAMURA Hidemi, MORITA Seiichi

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    Grant amount:\18720000 ( Direct expense: \14400000 、 Indirect expense:\4320000 )

    It is demonstrated that the function of LIPOzyme (Liposome+Enzyme) could be induced by the construction of catalytic sites on the liposome membrane as a model biomembrane. The LIPOzyme function could be classified by three categories : (i) electron transfer ; (ii) proton transfer ; (iii) nucleophilic/electrophilic reaction. The LIPOzyme function could be, herewith, controlled by the tuning of junk peptides, ligands or their metal complexes to achieve the desired function. Furthermore, the immobilization of LIPOzyme into the module was presented to develop the novel membrane module. This technique is a promising method to develop the reactor/separator based on the LIPOzyme function. Besides, the mapping of the physicochemical properties of junk peptides and the lipid composition could be performed to clarify the optimal condition for the desired functions of LIPOzyme, resulting in developing their database.

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  • Study on crystallization and polymorphism of amyloidgenic proteins on biomembranes

    Grant number:20760539  2008 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    SHIMANOUCHI Toshinori, GOTO Yuji, NODA Minoru

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    Amyloid firbril formation is similar to a crystallization. The amyloid fibrils fragmented in advance has been reported to play a role for a seeds. The seeds showed the variety of their growth behavior in the presence of the model biomembranes (liposomes). The addition of oxidative stress could lead to the formation of radiated amyloid fibrils (spherulite) and as well as the oxidized liposome could. It is, therefore, suggested that the liposome membranes determined the polymorphism of the amyloid.

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  • バイオメンブレン・クロマトグラフィーの創成

    Grant number:19656203  2007 - 2009

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    久保井 亮一, 馬越 大, 島内 寿徳, 森田 誠一

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    Grant amount:\3300000 ( Direct expense: \3300000 )

    「バイオメンブレン・クロマトグラフィー」を開発するために,最小分離基材となる「超機能性リポソーム(生体膜Mimics)」の調製を中心として,次の3段階に分けて研究を進めてきた:(第1段階)超機能性リポソームの調製ならびに機能評価;(第2段階)超機能性リポソームの固定化ならびに安定性評価;(第3段階)バイオメンブレン・クロマトグラフィーの基礎
    最終年度は,バイオメンブレン・クロマトグラフィーの基礎データ蓄積と,工学的な応用について検討した.特に,【高度分離】ならびに【反応分離】に主眼においた,下記の2つのケーススタディを対象として,基礎と応用を密接にリンクさせた.
    [ケーススタディ]
    (A)高度分離モード:Alzheimer症関連ペプチドの構造状態の解析
    (B)反応/分離モード:リポソームをコア材料とする臓器代替デバイス(リポソーム人工臓器)の開発
    Amyloid β-Peptideを始めとするアミロイド性タンパク質(ケーススタディ(A))や抗酸化酵素を始めとする機能性酵素(ケーススタディ(B))を対象として,ストレス負荷により分子認識モードを制御したバイオメンブレン・クロマトグラフィーを用いて網羅的に解析した.既存のクロマトグラフィー(イオン交換,アフィニティなど)で得られる情報と比較し,バイオメンブレン・クロマトグラフィーの特徴ならびにその利用方法を体系化した.

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  • Studies on biosensor device technology based on immobilization of intact liposome on Si-integrated sensor structure

    Grant number:19360162  2007 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    NODA Minoru, SHIMANOUCHI Toshinori, OKUYAMA Masanori, KUBOI Ryouichi, MURAKAMI Shuichi

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    Grant amount:\18980000 ( Direct expense: \14600000 、 Indirect expense:\4380000 )

    We have studied a series of biosensor device technologies based on immobilization of intact liposome on Si-integrated sensor structure. As a result, the immobilized liposome keeps its intact behavior more than 24hrs, meaning practically long time range for usual sensing. Based on the result, three type of liposome biosensor of microbolometer biothermochemical sensor, leakage current sensor and cantilever strain sensor are designed, fabricated and evaluated for detection of target proteins. Finally these sensors are confirmed to show their effective sensing performance.

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  • ストレス負荷バイオメンブレンによる遺伝子制御に関する研究

    Grant number:19656220  2007 - 2008

    日本学術振興会  科学研究費助成事業 萌芽研究  萌芽研究

    馬越 大, 久保井 亮一, 島内 寿徳, 土戸 哲明

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    Grant amount:\3300000 ( Direct expense: \3300000 )

    本研究課題の目的は、Membrane(生体膜/モデル生体膜)のストレス応答機能を利用する新規な遺伝子制御技術の開発である。ここでは、以下の3項目に関して検討している。
    (1) In vitro転写・翻訳プロセスにおけるリポソームの役割
    (2) In vivo合成プロセスにおける生体膜の役割
    (3) In process生産系への応用
    本年度では、特に(2)と(3)について検討した。種々の膜特性(電荷密度、流動性、ドメイン形成度など)を有するリポソーム共存系において、緑色蛍光タンパク質(GFP)の発現効率を検討した結果、翻訳されたペプチドとリポソーム間の静電相互作用を適切に制御する必要性が示唆された。また、転写の際メッセンジャーRNA(mRNA)からの遺伝子発現効率を検討した結果、mRNA-リポソーム間静電相互作用が遺伝子干渉効果に影響を及ぼすことが見出された。さらに、特定の酸化ストレスを負荷すると、遺伝子干渉効果が緩和できることを発見した。したがって、ストレス負荷生体膜が遺伝子発現過程を制御することが可能であることが示唆された。さらに、項目(3)について、Streptmyces griseus株による酵素キトサナーゼの生産プロセスにリポソームを利用した結果、キトサナーゼの膜上への提示が可能であり、膜の水和特性に応じて、キトサナーゼ活性を制御できることが示された。
    以上より、リポソーム(モデル生体膜)による遺伝子発現過程ならびに菌体による目的生産物(タンパク質)の生産プロセスの制御技術の枠組みを提案することができたと考えられる。

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  • ストレス応答型人工酵素リポソームの設計・開発

    Grant number:17656268  2005 - 2006

    日本学術振興会  科学研究費助成事業 萌芽研究  萌芽研究

    久保井 亮一, 馬越 大, 島内 寿徳, 吉本 誠, 森田 誠一

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    Grant amount:\3400000 ( Direct expense: \3400000 )

    本申請課題では,リポソーム界面を反応場として,環境条件の変動(ストレス)に応答して触媒活性を制御する新規な人工酵素の創成を目的とする.平成17年度は,環境の酸化ストレス状態を認識して活性を制御するための活性中心として,アミロイドβタンパク質やスーパーオキシドジスムダーゼ(SOD)を膜上に提示し,酵素様活性が発現することを示した.そこで,平成18年度は,ROS応答・制御型人工酵素リポソームの設計・開発を目的として,以下の2班にわけて検討を進めた.
    (a)人工SOD機能解析班:天然SODの活性中心を模倣した人工SODリポソームを設計・開発する.前年度に開発したPorphyrinやアミノ酸残基をベースとしたリガンドをリポソームに修飾する事により,リガンドの離合集散(クラスター形成)挙動とSOD活性とが相関することを見出した.したがって,リポソーム膜のストレス応答特性による活性制御の可能性が示唆された.(馬越,吉本,久保井)
    (b)リポソーム/センサ班:上記の機能性リガンドにより修飾した,あるいはその他の膜成分を変化させたリボソームを調製し,誘電分散解析法,あるいはリボソーム固定化QCM(水晶振動子)を用いて,そのドメイン形成挙動やタンパク質・ペプチドとの相互作用を解析した.その結果,温度変化などのストレス条件に応じてリガンドのクラスター形成挙動を制御することが可能であり,SOD活性を制御可能であることが示された.(島内,森田,久保井)
    上記の知見を総括して,当該研究期間において,リボソームのストレス応答特性に基づいて,活性中心としてのリガンドのクラスター形成挙動を制御することによりSOD活性を発現することが可能であることを示せた.さらに,人工酵素リボソームを設計・開発するための新規なアプローチ(Build-up型ならびにBreak-down型)を提案し,体系化の方針を明確にした.

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  • 人工臓器への応用を指向した生体機能集積型素子(プロテオリポソーム)の設計・開発

    Grant number:16760635  2004 - 2005

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    島内 寿徳

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    Grant amount:\2900000 ( Direct expense: \2900000 )

    本研究課題では,プロテオリポソームを,従来の研究用ツールだけでなく人工臓器などに対して工業的に利用してくための基盤的知見を獲得することを目的としている.しかしながら,従来のプロテオリポソームの調製法,安定性,操作性には克服しなければならない問題点があるのも事実である.そこで,平成16年度では,(プロテオ)リポソームの特性解析に基づき,操作条件下での(プロテオ)リポソームの機能評価と担体への固定化が原理的に可能であることを見出した.平成17年度は,(プロテオ)リポソーム膜上に種々の酵素(断片)を提示することで当該機能をリポソームに付与することを目的とした.さらに,固定化リポソームカラムの薬物排出能を検討し,人工臓器への応用の可能性について基礎的検討を行った.
    検出用分子素子を封入したリポソームを用いたセンサシステムにより,熱,pH,酸化ストレス条件下におけるタンパク質・リポソームの特性/相互作用解析を統一的に進めることが出来ることを示した.この知見に基づき,脂質膜・(モデル)生体膜のストレス応答ダイナミクス((脂質)膜界面ダイナミクス)に立脚したリポソーム材料調製・機能発現・ストレス有効利用型プロセスの設計を目指した新領域「メンブレン・ストレスバイオテクノロジー」を提案している.例えば,酸化ストレス消去系酵素であるスーパーオキシドジスムターゼ(断片)をリポソーム膜上に提示し,酸化ストレス消去能をリポソームに付与することに成功した.さらに,高周波誘電分散解析(周波数範囲1M-30GHz)などの手法を用いて,膜の水和状態の変化,ならびに,変性タンパク質や膜タンパク質の脂質膜への導入過程をモニタリングし,(プロテオ)リポソーム調製への指針を得ている.プロテオリポソームの固定化条件を最適化して得られる固定化リポソームクロマトグラフィーは,長期間安定に使用可能であり,人工臓器の操作条件(37℃)での安定した薬物排出能を発揮することが確認された.

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  • Development of Sensor System to Analyse and Diagnose the abnormality of Protein Conformation

    Grant number:15206089  2003 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    KUBOI Ryoichi, TSUCHIDO Tetsuaki, GOTO Yuji, UMAKOSHI Hiroshi, YOSHIMOTO Makoto, SHIMANOUCHI Toshinori

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    Grant amount:\43680000 ( Direct expense: \33600000 、 Indirect expense:\10080000 )

    The goal of this research project is to establish the analytical method of dynamic interaction between the structurally abnormal protein and biomembrane under the stress condition, especially focusing on the local hydrophobicity (LH) or structural fluctuation. Amyloid beta peptide (Aβ) has been employed as a case study of the system to analyze and characterize the structural abnormality of various proteins. During past two years, we have developed the sensor system of the structural abnormality of protein and have established the database on the protein abnormality under the stress condition. In 2005, the method to judge the structural abnormality of proteins has been investigated based on the previous findings. Based on the database, the suitable conditions (stress condition and lipid compositions) to regenerate the conformational abnormality of protein under the stress condition on the liposome surface have been clarified, resulting that the microdomain-like structure of the membrane surface was found to play an important role on the conformational abnormality of proteins/peptides. It was furthermore found that the microdomain-like structure could suitably be controlled by the control of the stress conditions (heating, pH and ionic strength) and the modification of membrane components (alcohols, fatty acids, cholesterol). Especially in the case of the cholesterol-modified liposome, the Aβ-membrane interaction was found to be controlled on the membrane in the presence of metal ion, Cu. The Aβ-Cu complex furthermore induced the metalloenzyme-like function(oxidation of cholesterol and dopamine). The above potential functions of Aβ on the membrane was found to be closely related to the stability of hydrogen-bond of the main chain of the Aβ in the hydrophobic nano environment inside the liposome membrane. By using the membrane chip arraying various stressed-liposomes on the chip, it was found that we could assess the structural abnormality of the protein. The above findings on the structural abnormality of protein were summarized together with the physiological role of amyloid fibril formation of Aβ on the biomembrane.

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  • タンパク質の構造異常に関わるストレス応答ダイナミクスの評価

    Grant number:14750637  2002 - 2003

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    島内 寿徳

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    Grant amount:\2900000 ( Direct expense: \2900000 )

    本申請課題では,次世代型ナノバイオチップによるタンパク質のストレス応答ダイナミクスの解析・構造異常性の機構解明を目指す.そのため,以下の項目に分けて検討した.
    1.リポソームのナノバイオチップへの応用
    リポソームの誘電分散解析を行ない,数10MHz付近に現われる誘電緩和がリン脂質分子の軸回転運動,ならびに脂質膜の流動性に対応することがわかった.この緩和特性とリポソームの膜特性((i)膜流動性,(ii)電荷密度,(iii)面・局所的疎水性)との間に対応関係を見出すことができた.さらに熱・pHなどのストレス条件下での誘電特性を評価し,リポソームのストレス応答特性データベース化を行なった.
    2.タンパク質のストレス応答の評価
    タンパク質をセンサ素子であるリポソームと共存させると,リポソームの緩和周波数の変化が認められた.リポソームの膜特性を表す緩和周波数の変化から,相互作用している各種タンパク質の変性/巻き戻り過程・多量体化・二次構造の変化の過程に関する情報をon-lineで得ることができた.また,1.で得たデータベースに基づき,電解質封入リポソームを固定化した電極を作成し,タンパク質との相互作用で誘導される電解質の漏出を電気化学的に計測することで,多変量解析によるパタン解析への展開の可能性も示す事ができた.タンパク質の構造状態を解析することができた.これらの手法を併用することで,れゆえ,生体膜上でのアミロイド性タンパク質の構造異常化に関するオンラインモニタリングの可能性を示す事ができた.

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  • Seminar in Environmental Process Engineering (2021academic year) Late  - その他

  • Seminar in Environmental Process Engineering (2021academic year) Prophase  - その他

  • Basic Experiments in Analytical Chemistry (2021academic year) 1st and 2nd semester  - 月7~9,木7~9

  • Basic Experiments in Analytical Chemistry (2021academic year) 1st and 2nd semester  - 月7~9,木7~9

  • Experiments and Exercises in Environmental Chemistry C (2021academic year) 3rd and 4th semester  - [第3学期]金7~9, [第4学期]金5~7

  • Experiments in Environmental Chemistry Ⅲ (2021academic year) 3rd and 4th semester  - [第3学期]金7~9, [第4学期]金5~7

  • Process Systems Engineering (2020academic year) Second semester  - 火1,火2

  • Academic Writing (2020academic year) Third semester  - 月7,月8

  • Academic Writing (2020academic year) Fourth semester  - 月7,月8

  • Process Systems Engineering (2020academic year) Second semester  - 火1,火2

  • Process Design for Separation (2020academic year) Late  - その他

  • Separation Engineering (2020academic year) 1st and 2nd semester  - 火4,火5

  • Separation Engineering Ⅱ (2020academic year) Second semester  - 火4,火5

  • Separation Engineering I (2020academic year) 1st semester  - 火4,火5

  • Chemical Engineering Ⅱ (2020academic year) Second semester  - 木1,木2

  • Chemical Engineering Ⅲ (2020academic year) Third semester  - 火1,火2

  • Chemical Engineering B (2020academic year) 2nd and 3rd semester  - [第2学期]木1,木2, [第3学期]火1,火2

  • Separation Engineering (2020academic year) Prophase  - 月4,月5

  • Advanced Chemistry and Materials in Liberal Arts (2020academic year) 1st semester  - 木6,木7

  • Special Research (2020academic year) Year-round  - その他

  • Seminar in Environmental Process Engineering (2020academic year) Prophase  - その他

  • Seminar in Environmental Process Engineering (2020academic year) Late  - その他

  • Seminar in Environmental Process Engineering (2020academic year) Late  - その他

  • Seminar in Environmental Process Engineering (2020academic year) Prophase  - その他

  • Basic Experiments in Analytical Chemistry (2020academic year) 1st and 2nd semester  - 月6,月7,月8,木6,木7,木8

  • Basic Experiments in Analytical Chemistry (2020academic year) 1st and 2nd semester  - 月6,月7,月8,木6,木7,木8

  • Experiments and Exercises in Environmental Chemistry C (2020academic year) 3rd and 4th semester  - 金6,金7,金8

  • Experiments in Environmental Chemistry Ⅲ (2020academic year) 3rd and 4th semester  - 金6,金7,金8

  • Introduction to Environmental Chemistry and Materials (2020academic year) 1st and 2nd semester  - 金6

  • Introduction to Environmental Chemistry and Materials I (2020academic year) 1st semester  - 金6

  • Introduction to Environmental Chemistry and Materials II (2020academic year) Second semester  - 金6

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