2021/07/12 更新

写真a

クボキ タクオ
窪木 拓男
KUBOKI Takuo
所属
医歯薬学域 教授
職名
教授

学位

  • 歯学博士 ( 岡山大学 )

研究キーワード

  • インプラント

  • Regenerative Medicine

  • 補綴歯学

  • 再生医学

  • Oral Rehabilitation

研究分野

  • ライフサイエンス / 補綴系歯学

  • ライフサイエンス / 常態系口腔科学

学歴

  • 岡山大学   Graduate School, Division of Dental Research  

    - 1990年

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  • 岡山大学    

    - 1990年

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    国名: 日本国

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  • 岡山大学   Faculty of Dentistry  

    - 1986年

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  • 岡山大学   歯学部   歯学

    - 1986年

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    国名: 日本国

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経歴

  • - 朝日大学 客員教授

    2017年

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  • 岡山大学

    2016年4月 - 2017年3月

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  • Vice President, Okayama University

    2016年4月 - 2017年3月

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  • - ハノイ医科大学 名誉教授

    2016年

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  • - Professor Emeritus,Hanoi Medical University

    2016年

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  • - Professor Emeritus,Haiphong University of Medicine and Pharmacy

    2013年

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  • - ハイフォン医科薬科大学 名誉教授

    2013年

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  • Dean,Dental School,Okayama University

    2012年 - 2016年

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  • 岡山大学歯学部 部長(学部長含む)   Dental School

    2012年 - 2016年

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  • 岡山大学医歯薬学総合研究科 副研究科長

    2009年 - 2012年

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  • 岡山大学岡山大学病院 副病院長(教育・研究担当)   Okayama University Hospital

    2007年 - 2009年

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  • - 岡山大学大学院医歯(薬)学総合研究科 教授

    2003年

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  • - Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2003年

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  • - Professor,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

    2003年

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  • - 岡山大学医歯薬学総合研究科 教授

    2003年

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  • 岡山大学大学院医歯学総合研究科 助教授

    2001年 - 2003年

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  • Associate Professor,Okayama University Graduate School of Medicine and Dentistry

    2001年 - 2003年

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  • 岡山大学歯学部 助教授   Dental School

    2000年 - 2001年

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  • Associate Professor,Okayama Unviersity Dental School

    2000年 - 2001年

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  • Visiting Scholar,University of California, Los Angeles, Advanced Tissue Science, NIH

    1997年 - 1998年

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  • カリフォルニア大学ロサンゼルス校 文部省在外研究員(短期)

    1997年 - 1998年

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  • Visiting Scholar,University of California, Los Angeles

    1994年 - 1995年

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  • カリフォルニア大学ロサンゼルス校 文部省在外研究員(長期)

    1994年 - 1995年

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  • 岡山大学歯学部附属病院 講師   Dental School

    1991年 - 2000年

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  • Senior Assistant Professor,Okayama Unviersity Dental Hospital

    1991年 - 2000年

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  • Research Associate,Okayama University Dental School

    1990年 - 1991年

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  • 岡山大学歯学部 助手   Dental School

    1990年 - 1991年

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所属学協会

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委員歴

  • 認知症と口腔機能研究会   世話人代表  

    2018年   

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    団体区分:学協会

    認知症と口腔機能研究会

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  • - 日本歯科医学会連合 大型研究推進委員会 委員(2013-)  

    2016年   

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  • 日本口腔インプラント学会   理事  

    2014年   

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    団体区分:学協会

    日本口腔インプラント学会

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  • - 日本歯科医学会 歯科診療ガイドラインライブラリー協議会 委員(2013-)  

    2013年   

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  • 森田奨学育英会   評議員  

    2013年   

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    団体区分:学協会

    森田奨学育英会

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  • 岡山歯学会   理事  

    2012年   

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    団体区分:学協会

    岡山歯学会

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  • 日本歯科薬物療法学会   編集査読委員会査読委員  

    2011年   

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    団体区分:学協会

    日本歯科薬物療法学会

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  • 日本口腔リハビリテーション学会   理事  

    2009年   

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    団体区分:学協会

    日本口腔リハビリテーション学会

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  • 日本接着歯学会   評議員  

    2008年   

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    団体区分:学協会

    日本接着歯学会

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  • 日本補綴歯科学会   理事,研究企画推進委員会委員  

    2008年   

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    団体区分:学協会

    日本補綴歯科学会

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  • 日本骨代謝学会   評議員  

    2007年   

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    団体区分:学協会

    日本骨代謝学会

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  • 国際歯科研究学会日本部会(JADR)   評議員  

    2007年   

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    団体区分:学協会

    国際歯科研究学会日本部会(JADR)

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  • 日本顎関節学会   理事  

    2007年   

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    団体区分:学協会

    日本顎関節学会

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  • 日本歯科理工学会   評議員  

    2005年   

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    団体区分:学協会

    日本歯科理工学会

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  • 日本歯科基礎医学会   会員  

    1997年   

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    団体区分:学協会

    日本歯科基礎医学会

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  • 日本IMZインプラント研究会   評議員  

    1996年 - 2002年   

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    団体区分:学協会

    日本IMZインプラント研究会

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  • 日本口腔科学会   会員  

    1996年   

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    団体区分:学協会

    日本口腔科学会

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論文

  • Tryptophan and Kynurenine Enhances the Stemness and Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro and In Vivo. 国際誌

    Hai Thanh Pham, Mitsuaki Ono, Emilio Satoshi Hara, Ha Thi Thu Nguyen, Anh Tuan Dang, Hang Thuy Do, Taishi Komori, Ikue Tosa, Yuri Hazehara-Kunitomo, Yuya Yoshioka, Yasutaka Oida, Kentaro Akiyama, Takuo Kuboki

    Materials (Basel, Switzerland)   14 ( 1 )   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aging tissues present a progressive decline in homeostasis and regenerative capacities, which has been associated with degenerative changes in tissue-specific stem cells and stem cell niches. We hypothesized that amino acids could regulate the stem cell phenotype and differentiation ability of human bone marrow-derived mesenchymal stromal cells (hBMSCs). Thus, we performed a screening of 22 standard amino acids and found that D-tryptophan (10 μM) increased the number of cells positive for the early stem cell marker SSEA-4, and the gene expression levels of OCT-4, NANOG, and SOX-2 in hBMSCs. Comparison between D- and L-tryptophan isomers showed that the latter presents a stronger effect in inducing the mRNA levels of Oct-4 and Nanog, and in increasing the osteogenic differentiation of hBMSCs. On the other hand, L-tryptophan suppressed adipogenesis. The migration and colony-forming ability of hBMSCs were also enhanced by L-tryptophan treatment. In vivo experiments delivering L-tryptophan (50 mg/kg/day) by intraperitoneal injections for three weeks confirmed that L-tryptophan significantly increased the percentage of cells positive for SSEA-4, mRNA levels of Nanog and Oct-4, and the migration and colony-forming ability of mouse BMSCs. L-kynurenine, a major metabolite of L-tryptophan, also induced similar effects of L-tryptophan in enhancing stemness and osteogenic differentiation of BMSCs in vitro and in vivo, possibly indicating the involvement of the kynurenine pathway as the downstream signaling of L-tryptophan. Finally, since BMSCs migrate to the wound healing site to promote bone healing, surgical defects of 1 mm in diameter were created in mouse femur to evaluate bone formation after two weeks of L-tryptophan or L-kynurenine injection. Both L-tryptophan and L-kynurenine accelerated bone healing compared to the PBS-injected control group. In summary, L-tryptophan enhanced the stemness and osteoblastic differentiation of BMSCs and may be used as an essential factor to maintain the stem cell properties and accelerate bone healing and/or prevent bone loss.

    DOI: 10.3390/ma14010208

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  • A comparison of colorimetric and visual methods for the assessment of masticatory performance with color-changeable chewing gum in older persons. 国際誌

    Yoshihiro Kugimiya, Yutaka Watanabe, Maki Shirobe, Yoshiko Motohashi, Keiko Motokawa, Ayako Edahiro, Yuki Ohara, Masahiro Ryu, Kentaro Igarashi, Daichi Hoshino, Junko Nakajima, Takayuki Ueda, Yu Taniguchi, Toru Ogawa, Kenji Maekawa, Katsushi Tamaki, Takuo Kuboki, Akihiko Kitamura, Shoji Shinkai, Hirohiko Hirano

    Journal of dental sciences   16 ( 1 )   380 - 388   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background/purpose: Color-changeable chewing gum is used for the evaluation of masticatory performance. However, it is currently unclear whether colorimetric and visual assessment methods yield consistent results. This study aimed to clarify the consistency between colorimetric and visual methods used for the evaluation of color changes in color-changeable chewing gum. Materials and methods: The sample comprised 644 older persons (mean age, 75.4 ± 6.4 years). The chewing gum was masticated 60 times at the participant's own chewing rate and then expectorated. The color of the chewing gum was evaluated with the ΔE values and a∗ values, measured using a colorimeter, and the 10 Color Shades (10CSh) and 5 Color Scales (5CSc), using visual evaluation. Spearman's correlation analysis was performed to examine the correlation between the results obtained by the four methods. The significance level was set at α = 0.05. Results: The ΔE values, a∗ values, 10CSh scores, and 5CSc scores were all significantly correlated. The highest correlation coefficient (0.979) was between the ΔE values and a∗ values. The lowest correlation coefficient (0.847) was between the a∗ values and 5CSc scores. Decreased masticatory performance was observed with increased age. Conclusion: Significant correlations were found for all four methods used in the assessment of masticatory performance with color-changeable chewing gum. While visually based assessments are valid, colorimetric methods are more sensitive to smaller changes in masticatory performance.

    DOI: 10.1016/j.jds.2020.08.010

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  • Six-year follow-up assessment of prosthesis survival and oral health-related quality of life in individuals with partial edentulism treated with three types of prosthodontic rehabilitation.

    Yoko Kurosaki, Aya Kimura-Ono, Takuya Mino, Hikaru Arakawa, Eri Koyama, Shinsuke Nakagawa, Ha Thi Thu Nguyen, Suguru Osaka, Mamiko Saeki, Hajime Minakuchi, Mitsuaki Ono, Kenji Maekawa, Takuo Kuboki

    Journal of prosthodontic research   2020年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: The purpose of the study was to compare the long-term performance of three prostheses for partial edentulism: implant-supported, fixed denture (IFD), fixed partial denture (FPD), and removable partial denture (RPD), in terms of prosthesis survival and oral health-related quality of life (OHRQoL). METHODS: The 138 patients in our previous study (Kimura et al., 2012) received one of the three prosthetic treatments and answered a validated OHRQoL questionnaire before and immediately after treatment. In the present study, the patients were followed up six years after treatment using medical records and OHRQoL examinations to evaluate prosthesis survival and change in OHRQoL. The cumulative survival rates were calculated using the Kaplan-Meier analysis. The Steel-Dwass test was used to compare the median OHRQoL scores at the three time points. RESULTS: For the 105 patients (66.8 ± 10.8 years, IFD/FPD/RPD: 58/27/20 patients) who successfully completed the follow-up assessments, the six-year estimated cumulative survival rates of the IFDs, FPDs, and RPDs were 94.7%, 77.4%, and 33.3%, respectively. The log-rank tests indicated that the survival curves were significantly different (IFDs vs. FPDs: p = 0.01; RPDs vs. IFDs, FPDs: p < 0.01). The median OHRQoL scores of the IFD group immediately after treatment and six years after treatment were significantly higher than those observed before treatment (p < 0.01). There was no significant difference in the median OHRQoL scores among the three time points in the RPD or FPD groups. CONCLUSIONS: IFDs showed significantly longer survival rates than FPDs and RPDs in partially edentulous patients. Only in the IFD patients was the OHRQoL level six years after treatment significantly higher than that b efore treatment.

    DOI: 10.2186/jpr.JPR_D_20_00095

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  • Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model. 国際誌

    Kyaw Thu Aung, Kentaro Akiyama, Masayoshi Kunitomo, Aung Ye Mun, Ikue Tosa, Ha Thi Thu Nguyen, Jiewen Zhang, Teisaku Kohno, Mitsuaki Ono, Emilio Satoshi Hara, Takuo Kuboki

    International journal of molecular sciences   21 ( 21 )   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Mesenchymal stem cells (MSCs) are known to play important roles in the repair of lost or damaged tissues and immunotolerance. On the other hand, aging is known to impair MSC function. However, little is currently known about how aged MSCs affect the host response to the local inflammatory condition and tissue deterioration in periodontitis, which is a progressive destructive disease of the periodontal tissue potentially leading to multiple tooth loss. In this study, we examined the relationship between aging-induced impairment of MSC function and the severity of periodontal tissue destruction associated with the decrease in host immunomodulatory response using a ligature-induced periodontitis model in young and aged mice. The results of micro computerized tomography (micro-CT) and histological analysis revealed a more severe bone loss associated with increased osteoclast activity in aged (50-week-old) mice compared to young (5-week-old) mice. Immunostaining analysis revealed that, in aged mice, the accumulation of inflammatory T and B cells was higher, whereas the percentage of platelet-derived growth factor receptor α (PDGFRα)+ MSCs, which are known to modulate the apoptosis of T cells, was significantly lower than in young mice. In vitro analysis of MSC function showed that the expression of surface antigen markers for MSCs (Sca-1, CD90, CD146), colony formation, migration, and osteogenic differentiation of aged MSCs were significantly declined compared to those of young MSCs. Moreover, a significantly higher proportion of aged MSCs were positive for the senescence-associated β galactosidase activity. Importantly, aged MSCs presented a decreased expression of FAS-L, which was associated with a lower immunomodulatory property of aged MSCs to induce T cell apoptosis in co-cultures compared with young MSCs. In summary, this is the first study showing that aging-induced impairment of MSC function, including immunomodulatory response, is potentially correlated with progressive periodontal tissue deterioration.

    DOI: 10.3390/ijms21218103

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  • Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments. 国際誌

    Shuji Nosho, Ikue Tosa, Mitsuaki Ono, Emilio Satoshi Hara, Kei Ishibashi, Akihiro Mikai, Yukie Tanaka, Aya Kimura-Ono, Taishi Komori, Kenji Maekawa, Takuo Kuboki, Toshitaka Oohashi

    International journal of molecular sciences   21 ( 21 )   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2) have been regarded as the major cytokines promoting bone formation, however, several studies have reported unexpected results with failure of bone formation or bone resorption of these growth factors. In this study, BMP-2 and FGF-2 adsorbed into atellocollagen sponges were transplanted into bone defects in the bone marrow-scarce calvaria (extramedullary environment) and bone marrow-abundant femur (medullary environment) for analysis of their in vivo effects not only on osteoblasts, osteoclasts but also on bone marrow cells. The results showed that BMP-2 induced high bone formation in the bone marrow-scarce calvaria, but induced bone resorption in the bone marrow-abundant femurs. On the other hand, FGF-2 showed opposite effects compared to those of BMP-2. Analysis of cellular dynamics revealed numerous osteoblasts and osteoclasts present in the newly-formed bone induced by BMP-2 in calvaria, but none were seen in either control or FGF-2-transplanted groups. On the other hand, in the femur, numerous osteoclasts were observed in the vicinity of the BMP-2 pellet, while a great number of osteoblasts were seen near the FGF-2 pellets or in the control group. Of note, FCM analysis showed that both BMP-2 and FGF-2 administrated in the femur did not significantly affect the hematopoietic cell population, indicating a relatively safe application of the two growth factors. Together, these results indicate that BMP-2 could be suitable for application in extramedullary bone regeneration, whereas FGF-2 could be suitable for application in medullary bone regeneration.

    DOI: 10.3390/ijms21217967

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  • BMP-2/β-TCP Local Delivery for Bone Regeneration in MRONJ-Like Mouse Model. 国際誌

    Akihiro Mikai, Mitsuaki Ono, Ikue Tosa, Ha Thi Thu Nguyen, Emilio Satoshi Hara, Shuji Nosho, Aya Kimura-Ono, Kumiko Nawachi, Takeshi Takarada, Takuo Kuboki, Toshitaka Oohashi

    International journal of molecular sciences   21 ( 19 )   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Medication-related osteonecrosis of the jaw (MRONJ) is a severe pathological condition associated mainly with the long-term administration of bone resorption inhibitors, which are known to induce suppression of osteoclast activity and bone remodeling. Bone Morphogenetic Protein (BMP)-2 is known to be a strong inducer of bone remodeling, by directly regulating osteoblast differentiation and osteoclast activity. This study aimed to evaluate the effects of BMP-2 adsorbed onto beta-tricalcium phosphate (β-TCP), which is an osteoinductive bioceramic material and allows space retention, on the prevention and treatment of MRONJ in mice. Tooth extraction was performed after 3 weeks of zoledronate (ZA) and cyclophosphamide (CY) administration. For prevention studies, BMP-2/β-TCP was transplanted immediately after tooth extraction, and the mice were administered ZA and CY for an additional 4 weeks. The results showed that while the tooth extraction socket was mainly filled with a sparse tissue in the control group, bone formation was observed at the apex of the tooth extraction socket and was filled with a dense connective tissue rich in cellular components in the BMP-2/β-TCP transplanted group. For treatment studies, BMP-2/β-TCP was transplanted 2 weeks after tooth extraction, and bone formation was followed up for the subsequent 4 weeks under ZA and CY suspension. The results showed that although the tooth extraction socket was mainly filled with soft tissue in the control group, transplantation of BMP-2/β-TCP could significantly accelerate bone formation, as shown by immunohistochemical analysis for osteopontin, and reduce the bone necrosis in tooth extraction sockets. These data suggest that the combination of BMP-2/β-TCP could become a suitable therapy for the management of MRONJ.

    DOI: 10.3390/ijms21197028

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  • Newly developed data-matching methodology for oral implant surgery allowing the automatic deletion of metal artifacts in 3D-CT images using new reference markers: A case report.

    Hiroaki Shimizu, Hikaru Arakawa, Takuya Mino, Yoko Kurosaki, Kana Tokumoto, Takuo Kuboki

    Journal of prosthodontic research   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PATIENTS: The patient was a 55-year-old woman with left upper molar free-end edentulism and 9 full cast metal crowns in her mouth. Three three-dimensional (3D) images were superimposed: a computed tomography (CT) image with the patient wearing the CT-matching template (CTMT) with six glass ceramic markers, which hardly generate any artifacts, on the template surface, and oral plaster model surfaces with and without CTMTs. Metal artifacts were automatically removed by a Boolean operation identifying unrealistic images outside the oral plaster model surface. After the preoperative simulation, fully guided oral implant surgery was performed. Two implant bodies were placed in the left upper edentulism. The placement errors calculated by comparing the preoperative simulation and actual implant placement were then assessed by a software program using the 3D-CT bone morphology as a reference. The 3D deviations between the preoperative simulation and actual placement at the entry of the implant body were a maximum 0.48 mm and minimum 0.26 mm. Those at the tip of the implant body were a maximum 0.56 mm and a minimum 0.25 mm. DISCUSSION: In this case, the maximum 3D deviations at the entry and tip section were less than in previous studies using double CT. CONCLUSIONS: Accurate image fusion utilizing CTMT with new reference markers was possible for a patient with many metal restorations. Using a surgical guide manufactured by the new matching methodology (modified single CT scan method), implant placement deviation can be minimized in patients with many metal restorations.

    DOI: 10.2186/jpr.JPOR_2019_429

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  • Number of functional teeth more strongly predicts all-cause mortality than number of present teeth in Japanese older adults. 査読

    Kenji Maekawa, Tomoko Ikeuchi, Shoji Shinkai, Hirohiko Hirano, Masahiro Ryu, Katsushi Tamaki, Hirofumi Yatani, Takuo Kuboki, Aya Kimura-Ono, Takeshi Kikutani, Takashi Suganuma, Yasunori Ayukawa, Tomoya Gonda, Toru Ogawa, Masanori Fujisawa, Shoichi Ishigaki, Yutaka Watanabe, Akihiko Kitamura, Yu Taniguchi, Yoshinori Fujiwara, Ayako Edahiro, Yuki Ohara, Junichi Furuya, Junko Nakajima, Kento Umeki, Kentaro Igarashi, Yasuhiro Horibe, Yoshihiro Kugimiya, Yasuhiko Kawai, Hideo Matsumura, Tetsuo Ichikawa, Shuji Ohkawa

    Geriatrics & gerontology international   20 ( 6 )   607 - 614   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    AIM: Previous studies on the association between intraoral conditions and mortality in community-dwelling older individuals reported that fewer present teeth (PT) are significant risk factors for mortality. However, how the number of PT relative to the number of functional teeth (FT), including both present and rehabilitated teeth, influences mortality has not been investigated fully. This study examined the impact of the number of FT on mortality among community-dwelling Japanese older adults. METHODS: This study was a retrospective, observational and population-based follow-up study, which examined 1188 older individuals who participated in an annual geriatric health examination from 2009 to 2015. The average follow-up period was 1697.0 ± 774.5 days. The primary outcome was all-cause mortality at follow-up. The numbers of PT and FT of each participant were counted during an oral examination. In addition, demographics, clinical variables, blood nutrient markers, physical functions and perceived masticatory function were measured. RESULTS: Kaplan-Meier analysis, followed by a log-rank test, revealed that fewer PT (P < 0.001) and FT (P = 0.002) were significantly associated with a reduced survival rate. Cox's proportional hazard analysis indicated that the number of FT, but not the number of PT, was a significant independent mortality risk factor after adjusting for demographics, clinical variables, nutrient markers and physical functioning (P = 0.036, hazard ratio: 2.089). CONCLUSIONS: Current results suggest that the number of FT more strongly predicts all-cause mortality than the number of PT among community-dwelling older adults. Further studies are necessary to consider the confounding of socioeconomic status and disability status. Geriatr Gerontol Int 2020; ••: ••-••.

    DOI: 10.1111/ggi.13911

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  • Association of masticatory muscle activity with sleep arousal and other concomitant movements during sleep. 査読 国際誌

    Haruna Miki, Hajime Minakuchi, Mayu Miyagi, Emilio Satoshi Hara, Shuji Shigemoto, Yoshitaka Suzuki, Kenji Maekawa, Yoshizo Matsuka, Glenn T Clark, Takuo Kuboki

    Journal of oral rehabilitation   47 ( 3 )   281 - 288   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    OBJECTIVE: This study aims to verify the associations among sleep bruxism (SB), sleep arousal (SA) and concurrent body movements. MATERIAL AND METHODS: Subjects underwent a standard overnight polysomnography test and audio-video recordings. Sleep quality was evaluated according to the Rechtschaffen and Kales criteria, while SA was determined as per the American Sleep Disorders Association criteria. Analyses were performed by an external institution after masking of the subjects' information. SB was assessed based on the presence/absence of rhythmic masticatory muscle activity (RMMA) episodes, which were identified by using electromyography of the masseter muscle. The observed simultaneous movements included lower leg movement (LLM), swallowing, face scratching, head movement, body movement, eye blinking, coughing, licking, sighing, body scratching, lip sucking, somniloquy and yawning. The LLM was determined visually, as well as through an increase in the tibialis electromyogram signal. Other movements were visually assessed using audio-video recordings. The incidences of all the simultaneous movements were compared between RMMA with intercurrent SA (SAwRMMA; RMMA episode derived from a masseter electromyogram showing more than 10% of maximum voluntary contraction) and SA without RMMA (SAw/oRMMA). RESULTS: Fourteen subjects were included in this study (females/males: 4/10, mean age: 31.5 ± 5.7 years). Among these, LLM, swallowing, body movement, licking, body scratching and lip sucking were frequently observed in SAwRMMA episodes than in SAw/oRMMA episodes, significantly. However, the non-specific simultaneous movements were higher observed in SAw/oRMMA episodes than that in SAwRMMA. CONCLUSION: Our results suggest that SB is concurrently activated with LLM in relation to arousal.

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  • Vibratory splint therapy for decreasing sleep clenching: A pilot study. 査読 国際誌

    Emilio Satoshi Hara, Andrea Lusvarghi Witzel, Hajime Minakuchi, Carlos Eduardo de Pitta, Rosane Tronquin Gallo, Masahiro Okada, Takuya Matsumoto, Takuo Kuboki, Marcelo Costa Bolzan

    Cranio : the journal of craniomandibular practice   38 ( 1 )   15 - 21   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Informa {UK} Limited  

    OBJECTIVE: To estimate the effect of a vibratory splint (VibS) in reducing sleep clenching (SC) and TMD pain. METHODS: Target sample was consecutive 19 TMD patients attending the Orofacial Pain Clinic at FFO-FOUSP. Patients used the VibS or acrylic occlusal splint (OS) as control for 14 days. Outcome variables were SC frequency and pain, assessed by a portable electromyography detector-analyzer (BiteStrip™) and 100 mm VAS, respectively. Statistical analyses were performed with two-way repeated measures ANOVA, and analysis of covariance (ANCOVA). RESULTS: VibS promoted a marked decrease, whereas acrylic OS increased SC frequency after two weeks of use. Due to a significant difference in initial VAS levels between VibS and control group, the effect of the two splints on TMD pain could not be clearly estimated. CONCLUSION: The results suggested that VibS can potentially be used to reduce SC frequency, although further studies with larger sample size are necessary to confirm these findings.

    DOI: 10.1080/08869634.2018.1488652

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  • Full Digital Workflow for Use of a Scanbody to Accurately Transfer Morphology From Provisional to Final Implant-Supported Fixed Restoration. 国際誌

    Takuya Mino, Yoko Kurosaki, Kenji Maekawa, Kana Tokumoto, Koji Izumi, Akihiro Ueda, Aya Kimura-Ono, Takuo Kuboki

    The Journal of oral implantology   45 ( 6 )   494 - 498   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1563/aaid-joi-D-19-00064

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  • Relationship between oral environment and development of pneumonia and acute viral respiratory infection in dependent older individuals.

    Haruki Tashiro, Takeshi Kikutani, Fumiyo Tamura, Noriaki Takahashi, Takashi Tohara, Kumiko Nawachi, Kenji Maekawa, Takuo Kuboki

    Geriatrics & gerontology international   19 ( 11 )   1136 - 1140   2019年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    AIM: The importance of oral care has been recognized for the prevention of airway infections in older individuals who require long-term care. The present prospective cohort study was carried out a decade ago to identify risk factors with numerous intraoral conditions as possible predictors involved in the onset of pneumonia and acute viral respiratory infection (AVRI) in older people requiring long-term care during a 6-month follow-up period (including winter). METHODS: This study included 1785 older individuals residing in 31 long-term care facilities in which dental hygienists were involved in instruction on daily oral care. Primary end-points were development of pneumonia and AVRI during the 6-month follow-up period. Several factors related to each participant's general condition, oral environment, swallowing function and vaccinations (or lack thereof) were evaluated by calibrated dentists and dental hygienists before the study onset. RESULTS: During the 6-month follow-up period, 74 participants (4.1%) developed pneumonia, and 28 participants (1.6%) developed AVRI. Cox proportional hazard analysis showed that poor nutritional status and the presence of dysphagia were significant risk factors for pneumonia onset. The presence of dry mouth and halitosis were significant risk factors for AVRI. CONCLUSIONS: This prospective multicenter cohort study identified poor nutritional status and dysphagia as independent risk factors for the development of pneumonia, and dry mouth and halitosis as independent risk factors for the development of AVRI in older people who require long-term care and who routinely receive professional oral care. Geriatr Gerontol Int 2019; 19: 1136-1140.

    DOI: 10.1111/ggi.13784

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  • Type XVIII Collagen Modulates Keratohyalin Granule Formation and Keratinization in Oral Mucosa. 査読 国際誌

    Ha Thi Thu Nguyen, Mitsuaki Ono, Emilio Satoshi Hara, Taishi Komori, Midori Edamatsu, Tomoko Yonezawa, Aya Kimura-Ono, Kenji Maekawa, Takuo Kuboki, Toshitaka Oohashi

    International journal of molecular sciences   20 ( 19 )   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Epithelial keratinization involves complex cellular modifications that provide protection against pathogens and chemical and mechanical injuries. In the oral cavity, keratinized mucosa is also crucial to maintain healthy periodontal or peri-implant tissues. In this study, we investigated the roles of type XVIII collagen, a collagen-glycosaminoglycan featuring an extracellular matrix component present in the basement membrane, in oral mucosal keratinization. Histological analysis of keratinized and non-keratinized oral mucosa showed that type XVIII collagen was highly expressed in keratinized mucosa. Additionally, a 3D culture system using human squamous carcinoma cells (TR146) was used to evaluate and correlate the changes in the expression of type XVIII collagen gene, COL18A1, and epithelial keratinization-related markers, e.g., keratin 1 (KRT1) and 10 (KRT10). The results showed that the increase in COL18A1 expression followed the increase in KRT1 and KRT10 mRNA levels. Additionally, loss-of-function analyses using silencing RNA targeting COL18A1 mRNA and a Col18-knockout (KO) mouse revealed that the absence of type XVIII collagen induces a dramatic decrease in KRT10 expression as well as in the number and size of keratohyalin granules. Together, the results of this study demonstrate the importance of type XVIII collagen in oral mucosal keratinization.

    DOI: 10.3390/ijms20194739

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  • Postnatal Runx2 deletion leads to low bone mass and adipocyte accumulation in mice bone tissues. 査読 国際誌

    Ikue Tosa, Daisuke Yamada, Misa Yasumatsu, Eiichi Hinoi, Mitsuaki Ono, Toshitaka Oohashi, Takuo Kuboki, Takeshi Takarada

    Biochemical and biophysical research communications   516 ( 4 )   1229 - 1233   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Global gene deletion studies have established that Runt-related transcription factor-2 (Runx2) is essential during skeletogenesis for osteoblastic differentiation in both intramembranous and endochondral ossification processes. However, the postnatal significance of Runx2 in vivo is poorly understood because a global Runx2 deletion causes perinatal lethality. In this study, we generated tamoxifen-induced Runx2 global deficient mice by crossing Runx2flox mice with ROSA26-CreERT2 mice (Rosa26-CreERT2; Runx2flox/flox). Four-week-old mice were intraperitoneally treated with tamoxifen for five consecutive days, sacrificed, and analyzed six weeks after tamoxifen administration. Deletion of Runx2 led to low bone mass, which is associated with decreased bone formation and bone resorption as well as excessive bone marrow adiposity. Collectively, postnatal Runx2 absolutely plays an important role in maintaining the homeostasis of bone tissues not only in bone mass, but also in the bone marrow environment.

    DOI: 10.1016/j.bbrc.2019.07.014

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  • Loss of oral self-care ability results in a higher risk of pneumonia in older inpatients: A prospective cohort study in a Japanese rural hospital. 国際誌

    Aya Fujiwara, Hajime Minakuchi, Junji Uehara, Haruna Miki, Mami Inoue-Minakuchi, Aya Kimura-Ono, Kumiko Nawachi, Kenji Maekawa, Takuo Kuboki

    Gerodontology   36 ( 3 )   236 - 243   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: To identify significant risk factors associated with incidence of mortality and pneumonia in whole-community-based older inpatients resident in Japanese rural region. METHODS: Patients older than 65 years admitted between 1 April and 15 April 2010 to a core hospital located in a rural region were exhaustively recruited, and incidence of mortality and pneumonia during the 32-month follow-up period were evaluated. Independent variables at baseline measurement included age, gender, body mass index, Charlson comorbidity index, functional dependency, oral self-care ability index, number of remaining teeth, hyposalivation and nutritional status. Dependent variables were incidence of mortality and pneumonia. Survival and non-pneumonia curves were drawn using Kaplan-Meier analysis. Cox proportional hazards analysis was performed to identify the risk factors related to incidence of mortality and pneumonia. RESULTS: The survival rate of 46 patients (male/female: 11/35; mean age: 83.8 ± 6.8 years) was 52.1%, and the incidence of pneumonia was 60.9%. Malnutrition and gender (male) were identified as significant risk factors for mortality (odds ratio [OR]: 8.18 and 4.90; 95% confidence interval [CI]: 1.77-37.3 and 1.50-16.0; P < 0.01 and <0.01, respectively). Loss of oral self-care ability and gender (male) were identified as significant risk factors for incidence of pneumonia (OR: 8.97 and 4.58; 95% CI: 1.70-47.4 and 1.50-14.0; P = 0.01 and <0.01, respectively). CONCLUSIONS: Malnutrition and loss of oral self-care ability were significant risk factors for incidence of mortality and pneumonia, respectively. In response, supplying nutrition with appropriate diet and personalised oral care might contribute to reduction in mortality and prevention of pneumonia.

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  • Fibronectin-induced ductal formation in salivary gland self-organization model. 国際誌

    Mahmoud Farahat, Gulsan A S Kazi, Hiroaki Taketa, Emilio S Hara, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

    Developmental dynamics : an official publication of the American Association of Anatomists   248 ( 9 )   813 - 825   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Recent advances in tissue regeneration approaches including 3D organoids, were based on various 3D organogenesis models. However, 3D models are generally technique-sensitive and time-consuming. Thus, we utilized an existing model of submandibular salivary gland (SMG) to modify a simple and highly reproducible in vitro 3D culture model of primary SMG cells self-organization into a well-developed cell spheroid inside Matrigel substrate. We used this model to observe the collective multicellular behavior during spheroid formation. Further, we applied various quantitative approaches including real-time live imaging and immune histochemical image analysis to dissect the cellular dynamics during tissue patterning. RESULTS: On a time-scale of hours, we observed marked size and shape transformations in the developed 3D spheroid which resulted in a spatially-controlled growth differential from the canter to the periphery of the formed aggregates. Moreover, we investigated the effect of fibronectin (FN) on SMG cells self-organization using our simplified culture model. Interestingly, we discovered a novel role of FN in inducing duct-like elongation during initial stages of SMG bud formation. CONCLUSION: This in vitro model provides an excellent tool for analyzing the intercellular dynamics during early SMG tissue development as well as revealing a novel role of FN in SMG ductal expansion.

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  • Fifteen-year survival of resin-bonded vs full-coverage fixed dental prostheses. 査読

    T Yoshida, Y Kurosaki, A Mine, A Kimura-Ono, T Mino, S Osaka, S Nakagawa, K Maekawa, T Kuboki, H Yatani, A Yamashita

    Journal of prosthodontic research   63 ( 3 )   374 - 382   2019年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: We clarified cumulative survival and event-free rates of resin-bonded fixed dental prostheses (RBFDPs) and compared them to those of fixed dental prostheses (FDPs) to refine risk factors for non-survival/event and use of tooth extraction after the period of non-survival/event. METHODS: Study subjects were selected among all patients who consecutively attended the Fixed Prosthodontic Clinic of Okayama University Hospital. Eligible patients were those who received 3-unit metal-framed 2-retainer (wing-wing) RBFDPs or conventional full-coverage FDPs (RBFDPs/FDPs: 129/177 prostheses). Data were analyzed by Kaplan-Meier analysis with the log-rank test, Mann-Whitney test, chi-square test, and Cox proportional hazards analysis. RESULTS: The 15-year cumulative survival rates were 66.5% for the RBFDP group and 61.6% for the FDP group, which were not significantly different (p = 0.59). The 15-year cumulative event-free rates were 53.4% for the RBFDP group and 59.2% for the FDP group, which were not significantly different (p = 0.52). No significant risk factors related to non-survival and event-free of RBFDPs/FDPs were identified in the analysis model using treatment method, sex, age, number of remaining teeth, and treatment site as explanatory variables. The number of cases in which RBFDPs/FDPs resulted in non-survival due to abutment tooth extraction was significantly lower in RBFDPs (p < 0.01). Further, the abutment tooth as a non-vital tooth was identified as a risk factor for RBFDPs/FDPs resulting in non-survival due to abutment tooth extraction. CONCLUSIONS: The present study is the first to indicate RBFDP as a prosthetic treatment option which should be selected for patients with slight or no abutment tooth decay.

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  • Biomimetic mineralization using matrix vesicle nanofragments. 査読 国際誌

    Yosuke Kunitomi, Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takuo Kuboki, Takayoshi Nakano, Hiroshi Kamioka, Takuya Matsumoto

    Journal of biomedical materials research. Part A   107 ( 5 )   1021 - 1030   2019年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. © 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021-1030, 2019.

    DOI: 10.1002/jbm.a.36618

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  • Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells. 査読 国際誌

    Yuri Hazehara-Kunitomo, Emilio Satoshi Hara, Mitsuaki Ono, Kyaw Thu Aung, Keiko Komi, Hai Thanh Pham, Kentaro Akiyama, Masahiro Okada, Toshitaka Oohashi, Takuya Matsumoto, Takuo Kuboki

    International journal of molecular sciences   20 ( 5 )   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A deeper understanding of the detailed mechanism of in vivo tissue healing is necessary for the development of novel regenerative therapies. Among several external factors, environmental pH is one of the crucial parameters that greatly affects enzyme activity and cellular biochemical reactions involving tissue repair and homeostasis. In this study, in order to analyze the microenvironmental conditions during bone healing, we first measured the pH in vivo at the bone healing site using a high-resolution fiber optic pH microsensor directly in femur defects and tooth extraction sockets. The pH was shown to decrease from physiological 7.4 to 6.8 during the initial two days of healing (inflammatory phase). In the same initial stages of the inflammatory phase of the bone healing process, mesenchymal stem cells (MSCs) are known to migrate to the healing site to contribute to tissue repair. Therefore, we investigated the effect of a short-term acidic (pH 6.8) pre-treatment on the stemness of bone marrow-derived MSCs (BMSCs). Interestingly, the results showed that pre-treatment of BMSCs with acidic pH enhances the expression of stem cell markers (OCT-4, NANOG, SSEA-4), as well as cell viability and proliferation. On the other hand, acidic pH decreased BMSC migration ability. These results indicate that acidic pH during the initial stages of bone healing is important to enhance the stem cell properties of BMSCs. These findings may enable the development of novel methods for optimization of stem cell function towards tissue engineering or regenerative medicine.

    DOI: 10.3390/ijms20051097

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  • The BMP-2 mutant L51P: a BMP receptor IA binding-deficient inhibitor of noggin. 査読

    Hany Mohamed Khattab, Satoshi Kubota, Masaharu Takigawa, Takuo Kuboki, Walter Sebald

    Journal of bone and mineral metabolism   37 ( 2 )   199 - 205   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The antagonist-specific regulation in tissue engineering constitutes important attempts to achieve an improved and rapid bone regeneration by controlling the natural biological response of the natural body growth factors. L51P is molecularly engineered bone morphogentic protein-2 (BMP-2) variant with a substitution of the 51st leucine with a proline residue. L51P is deficient in BMP receptor binding, but maintains its structure and affinity for inhibitory proteins such as noggin, chordin, and gremlin. These modifications convert the BMP-2 variant L51P into a receptor-inactive inhibitor of BMP antagonists. This current approach may prevent the uncontrolled bone overgrowth using high concentration of BMPs and thus regulates the possible growth factor's high-dose side effects. Exploring of L51P biological functions is required to broad our understanding of BMP mutant biological functions and their potential clinical applications. The progress of L51P researches would hopefully lead to the development of multiple applications for using the L51P in bone and fracture healing disorders.

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  • Bone Marrow Cells Inhibit BMP-2-Induced Osteoblast Activity in the Marrow Environment. 国際誌

    Ha Thi Nguyen, Mitsuaki Ono, Yasutaka Oida, Emilio Satoshi Hara, Taishi Komori, Kentaro Akiyama, Ha Thi Thu Nguyen, Kyaw Thu Aung, Hai Thanh Pham, Ikue Tosa, Takeshi Takarada, Koichi Matsuo, Toshihide Mizoguchi, Toshitaka Oohashi, Takuo Kuboki

    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research   34 ( 2 )   327 - 332   2019年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bone morphogenetic protein 2 (BMP-2) is widely known as a potent growth factor that promotes bone formation. However, an increasing number of studies have demonstrated side effects of BMP-2 therapy. A deeper understanding of the effect of BMP-2 on cells other than those involved directly in bone remodeling is of fundamental importance to promote a more effective delivery of BMP-2 to patients. In this study, we aimed to investigate the effect of BMP-2 in the marrow environment. First, BMP-2 adsorbed onto titanium implants was delivered at the tooth extraction socket (marrow-absent site) or in the mandible marrow of beagle dogs. BMP-2 could induce marked bone formation around the implant at the tooth extraction socket. Surprisingly, however, no bone formation was observed in the BMP-2-coated titanium implants inserted in the mandible marrow. In C57BL/6 mice, BMP-2 adsorbed in freeze-dried collagen pellets could induce bone formation in marrow-absent calvarial bone. However, similar to the canine model, BMP-2 could not induce bone formation in the femur marrow. Analysis of osteoblast differentiation using Col1a1(2.3)-GFP transgenic mice revealed a scarce number of osteoblasts in BMP-2-treated femurs, whereas in the control group, osteoblasts were abundant. Ablation of femur marrow recovered the BMP-2 ability to induce bone formation. In vitro experiments analyzing luciferase activity of C2C12 cells with the BMP-responsive element and alkaline phosphatase activity of MC3T3-E1 osteoblasts further revealed that bone marrow cells inhibit the BMP-2 effect on osteoblasts by direct cell-cell contact. Collectively, these results showed that the effect of BMP-2 in inducing bone formation is remarkably repressed by marrow cells via direct cell-cell contact with osteoblasts; this opens new perspectives on the clarification of the side-effects associated with BMP-2 application. © 2018 American Society for Bone and Mineral Research.

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  • Digital transfer of the subgingival contour and emergence profile of the provisional restoration to the final bone-anchored fixed restoration. 査読

    Yoko Kurosaki, Takuya Mino, Kenji Maekawa, Koji Izumi, Takuo Kuboki

    Journal of prosthodontic research   63 ( 1 )   125 - 129   2019年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: This report was written to introduce an attempt at clinical application of our newly developed digital workflow to reproduce the morphology of the subgingival contour and the emergence profile of the provisional restoration within the final bone-anchored fixed restoration, using a bounded unilateral edentulous case. METHODS: This digital workflow involves superimposition of the composite images of two specific types of working casts onto the working cast for the provisional restoration namely, a split cast screwed with a titanium base and a split cast screwed with a provisional restoration and integrating these with the whole intraoral surface image, in which the provisional restoration was present. The final restoration fabricated using this technique could be installed without any clinical problems. The results of in silico analysis revealed that the cubic volume ratio of the total discrepancy between the provisional and the final restorations was only 2.4%. Further, sufficient oral hygiene was maintained and the patient was satisfied with the outcome of the treatment. CONCLUSIONS: This technical report suggests that our newly developed digital workflow provided clinical applicability and may enable accurate transfer of the morphology of the subgingival contour and emergence profile of the provisional to the final bone-anchored fixed restoration.

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  • A multi-centered epidemiological study evaluating the validity of the treatment difficulty indices developed by the Japan Prosthodontic Society 査読

    Takuo Kuboki, Tetsuo Ichikawa, Kazuyoshi Baba, Masanori Fujisawa, Hironobu Sato, Hideki Aita, Shigeto Koyama, Masayuki Hideshima, Yuji Sato, Hiroyuki Wake, Aya Kimura-Ono, Kan Nagao, Yorika Kodaira-Ueda, Katsushi Tamaki, Shinsuke Sadamori, Kazuhiro Tsuga, Yasuhiro Nishi, Takashi Sawase, Hisashi Koshino, Shinichi Masumi, Kaoru Sakurai, Kanji Ishibashi, Takashi Ohyama, Yasumasa Akagawa, Toshihiro Hirai, Keiichi Sasaki, Kiyoshi Koyano, Hirofumi Yatani, Hideo Matsumura

    Annals of Japan Prosthodontic Society   11 ( 4 )   355 - 375   2019年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Prosthodontic Society  

    DOI: 10.2186/ajps.11.355

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  • DNA Methylation-Based Regulation of Human Bone Marrow-Derived Mesenchymal Stem/Progenitor Cell Chondrogenic Differentiation. 査読 国際誌

    Yu Nomura, Emilio Satoshi Hara, Yuya Yoshioka, Há Thi Nguyen, Shuji Nosho, Taishi Komori, Kei Ishibashi, Toshitaka Oohashi, Mitsuaki Ono, Takuo Kuboki

    Cells, tissues, organs   207 ( 3-4 )   115 - 126   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Stem cells have essential applications in in vitro tissue engineering or regenerative medicine. However, there is still a need to understand more deeply the mechanisms of stem cell differentiation and to optimize the methods to control stem cell function. In this study, we first investigated the activity of DNA methyltransferases (DNMTs) during chondrogenic differentiation of human bone marrow-derived mesenchymal stem/progenitor cells (hBMSCs) and found that DNMT3A and DNMT3B were markedly upregulated during hBMSC chondrogenic differentiation. In an attempt to understand the effect of DNMT3A and DNMT3B on the chondrogenic differentiation of hBMSCs, we transiently transfected the cells with expression vectors for the two enzymes. Interestingly, DNMT3A overexpression strongly enhanced the chondrogenesis of hBMSCs, by increasing the gene expression of the mature chondrocyte marker, collagen type II, more than 200-fold. Analysis of the methylation condition in the cells revealed that DNMT3A and DNMT3B methylated the promoter sequence of early stem cell markers, NANOG and POU5F1(OCT-4). Conversely, the suppression of chondrogenic differentiation and the increase in stem cell markers of hBMSCs were obtained by chemical stimulation with the demethylating agent, 5-azacitidine. Loss-of-function assays with siRNAs targeting DNMT3A also significantly suppressed the chondrogenic differentiation of hBMSCs. Together, these results not only show the critical roles of DNMTs in regulating the chondrogenic differentiation of hBMSCs, but also suggest that manipulation of DNMT activity can be important tools to enhance the differentiation of hBMSCs towards chondrogenesis for potential application in cartilage tissue engineering or cartilage regeneration.

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  • Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu. 査読 国際誌

    Emilio Satoshi Hara, Masahiro Okada, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

    Journal of materials chemistry. B   6 ( 38 )   6153 - 6161   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Royal Society of Chemistry ({RSC})  

    Bone is a sophisticated organic-inorganic hybrid material, whose formation involves a complex spatio-temporal sequence of events regulated by the cells. A deeper understanding of the mechanisms behind bone mineralization at different size scales, and using a multidisciplinary approach, may uncover novel pathways for the design and fabrication of functional bone tissue in vitro. The objectives of this study were first to investigate the environmental factors that prime initial mineralization using the secondary ossification center as an in vivo model, and then to apply the obtained knowledge for rapid in vitro synthesis of bone-like tissue. First, the direct and robust measurement of pH showed that femur epiphysis is alkaline (pH ≅ 8.5) at the initial mineral stage at post-natal day 6. We showed that the alkaline milieu is decisive not only for alkaline phosphatase activity, which precedes mineral formation at P6, but also for determining initial mineral precipitation and spherical morphology. Next, engineering approaches were used to synthesize bone-like tissue based on alkaline milieu and artificial chondrocyte membrane nanofragments, previously shown to be the nucleation site for mineral formation. Interestingly, mineralization using artificial cell membrane nanofragments was achieved in just 1 day. Finally, ex vivo culture of femur epiphysis in alkaline pH strongly induced chondrocyte burst, which was previously shown to be the origin of chondrocyte membrane nanofragments, and also enhanced mineral formation. Taken together, these findings not only shed more light on the microenvironmental conditions that prime initial bone formation in vivo, but they also show that alkaline milieu can be used as an important factor for enhancing methods for in vitro synthesis of bone tissue.

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  • CCN4/WISP1 controls cutaneous wound healing by modulating proliferation, migration and ECM expression in dermal fibroblasts via α5β1 and TNFα 国際誌

    窪木 拓男

    Matrix Biology   68-69   533 - 546   2018年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.matbio.2018.01.004

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  • 多職種連携教育の導入・充実を目指して 全国29歯科大学、歯学部多職種連携教育アンケート集計結果 査読

    片岡 竜太, 浅沼 直樹, 窪木 拓男, 越野 寿, 澤瀬 隆, 角 忠輝, 筒井 睦

    日本歯科医学教育学会総会・学術大会プログラム・抄録集   37回   76 - 76   2018年7月

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    記述言語:日本語   出版者・発行元:(一社)日本歯科医学教育学会  

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  • The BMP-2 mutant L51P: a BMP receptor IA binding-deficient inhibitor of noggin 査読

    Khattab HM, Kubota S, Takigawa M, Kuboki T, Sebald W

    J Bone Miner Metab.   128 ( 3 )   181 - 196   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s00774-018-0925-0

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  • A multi-centered epidemiological study evaluating the validity of the treatment difficulty indices developed by the Japan Prosthodontic Society. 査読

    Takuo Kuboki, Tetsuo Ichikawa, Kazuyoshi Baba, Masanori Fujisawa, Hironobu Sato, Hideki Aita, Shigeto Koyama, Masayuki Hideshima, Yuji Sato, Hiroyuki Wake, Aya Kimura-Ono, Kan Nagao, Yorika Kodaira-Ueda, Katsushi Tamaki, Shinsuke Sadamori, Kazuhiro Tsuga, Yasuhiro Nishi, Takashi Sawase, Hisashi Koshino, Shin-Ichi Masumi, Kaoru Sakurai, Kanji Ishibashi, Takashi Ohyama, Yasumasa Akagawa, Toshihiro Hirai, Keiichi Sasaki, Kiyoshi Koyano, Hirofumi Yatani, Hideo Matsumura

    Journal of prosthodontic research   62 ( 2 )   162 - 170   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier {BV}  

    PURPOSE: The Japan Prosthodontic Society developed a multi-axis assessment protocol to evaluate the complex variations in patients who need prosthodontic care, and to classify the level of treatment difficulty. A previous report found the protocol to be sufficiently reliable. The purpose of this multi-center cohort study was to evaluate the validity of this multi-axis assessment protocol. METHODS: The treatment difficulty was evaluated using the multi-axis assessment protocol before starting prosthodontic treatment. The time required for active prosthodontic treatment, medical resources such as treatment cost, and changes in the oral health-related QOL before and after treatment, were evaluated after treatment completion. The construct validity of this protocol was assessed by the correlation between the dentist's pre-operative subjective assessment of the treatment difficulty, and the level of difficulty determined by this protocol. The predictive validity was assessed estimating the correlations between a "comprehensive level of treatment difficulty" based on the four axes of this protocol and total treatment cost, total treatment time, and changes in the oral health-related QOL before and after treatment. RESULTS: The construct validity of this protocol was well documented except for psychological assessment. Regarding the predictive validity, the comprehensive level of treatment difficulty assessed before treatment was significantly correlated with the three surrogate endpoints known to be related to the treatment difficulty (total treatment cost, treatment time, and improvement in the oral health-related QOL). To further clarify the validity of the protocol according to patients' oral condition, a subgroup analysis by defects was performed. Analyses revealed that treatment difficulty assessment before treatment was significantly related to one or two surrogate endpoints in the fully edentulous patients and the partially edentulous patients. No significant relationship was observed in the patients with mixture of full/partial edentulism and the patients with teeth problems, possibly due to the small sample size in these groups. CONCLUSION: This study revealed that the multi-axis assessment protocol was sufficiently valid to predict the level of treatment difficulty in prosthodontic care in patients with fully edentulous defects and with partially edentulous defects.

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  • Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium. 国際誌

    窪木 拓男

    Scientific Reports   8 ( 1 )   2612 - 2612   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-018-21000-0

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  • Chondrocyte burst promotes space for mineral expansion. 査読 国際誌

    Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Letycia Mary Iida, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

    Integrative biology : quantitative biosciences from nano to macro   10 ( 1 )   57 - 66   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Royal Society of Chemistry ({RSC})  

    Analysis of tissue development from multidisciplinary approaches can result in more integrative biological findings, and can eventually allow the development of more effective bioengineering methods. In this study, we analyzed the initial steps of mineral formation during secondary ossification of mouse femur based on biological and bioengineering approaches. We first found that some chondrocytes burst near the mineralized area. External factors that could trigger chondrocyte burst were then investigated. Chondrocyte burst was shown to be modulated by mechanical and osmotic pressure. A hypotonic solution, as well as mechanical stress, significantly induced chondrocyte burst. We further hypothesized that chondrocyte burst could be associated with space-making for mineral expansion. In fact, ex vivo culture of femur epiphysis in hypotonic conditions, or under mechanical pressure, enhanced mineral formation, compared to normal culture conditions. Additionally, the effect of mechanical pressure on bone formation in vivo was investigated by immobilization of mouse lower limbs to decrease the body pressure onto the joints. The results showed that limb immobilization suppressed bone formation. Together, these results suggest chondrocyte burst as a novel fate of chondrocytes, and that manipulation of chondrocyte burst with external mechano-chemical stimuli could be an additional approach for cartilage and bone tissue engineering.

    DOI: 10.1039/c7ib00130d

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  • Bioinspired Mineralization Using Chondrocyte Membrane Nanofragments 査読

    Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

    ACS Biomaterials Science &. Engineering   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model. 国際誌

    Mitsuaki Ono, Masamitsu Oshima, Miho Ogawa, Wataru Sonoyama, Emilio Satoshi Hara, Yasutaka Oida, Shigehiko Shinkawa, Ryu Nakajima, Atsushi Mine, Satoru Hayano, Satoshi Fukumoto, Shohei Kasugai, Akira Yamaguchi, Takashi Tsuji, Takuo Kuboki

    Scientific reports   7   44522 - 44522   2017年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Nature  

    Whole-organ regeneration has great potential for the replacement of dysfunctional organs through the reconstruction of a fully functional bioengineered organ using three-dimensional cell manipulation in vitro. Recently, many basic studies of whole-tooth replacement using three-dimensional cell manipulation have been conducted in a mouse model. Further evidence of the practical application to human medicine is required to demonstrate tooth restoration by reconstructing bioengineered tooth germ using a postnatal large-animal model. Herein, we demonstrate functional tooth restoration through the autologous transplantation of bioengineered tooth germ in a postnatal canine model. The bioengineered tooth, which was reconstructed using permanent tooth germ cells, erupted into the jawbone after autologous transplantation and achieved physiological function equivalent to that of a natural tooth. This study represents a substantial advancement in whole-organ replacement therapy through the transplantation of bioengineered organ germ as a practical model for future clinical regenerative medicine.

    DOI: 10.1038/srep44522

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  • Assessment of CCN2 Independent Modules Regenerative Capacity on Osteoarthritis and Further Selecting the Most Suitable Among them as a Potential Therapeutic Drug 査読

    Abdelkader Tarek, Aoyama Eriko, Nishida Takashi, Hattori Takako, Janune Danilo, Hara Emilio S, Ono Mitsuaki, Tabata Yasuhiko, Kuboki Takuo, Kubota Satoshi, Takigawa Masaharu

    FASEB JOURNAL   30   2016年4月

  • Assessment of CCN2 Independent Modules Regenerative Capacity on Osteoarthritis and Further Selecting the Most Suitable Among them as a Potential Therapeutic Drug 査読

    Abdelkader Tarek, Aoyama Eriko, Nishida Takashi, Hattori Takako, Janune Danilo, Hara Emilio S, Ono Mitsuaki, Tabata Yasuhiko, Kuboki Takuo, Kubota Satoshi, Takigawa Masaharu

    FASEB JOURNAL   30   2016年4月

  • Highly dynamic self-organizing SMG morphogenesis in 3D culture 査読

    Mahmoud Farahat, Gulsan Ara Sathi, Hiroaki Taketa, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

    2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015   2016年3月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:Institute of Electrical and Electronics Engineers Inc.  

    Biological tissues have specific cell patterns and well-organized structures which result from various organogenesis processes. Among which is branching morphogenesis which is the key developmental processes for many glandular tissues including lungs, kidney, salivary gland and liver. Many approaches were suggested to regenerate salivary gland tissue in vitro. Here, we developed a 3D culture technique to study and recapitulate the dynamic forces generated during SMG organogenesis by utilizing time lapse live imaging to monitor the cellular migration and reorganization process of both epithelium and mesenchyme cells.

    DOI: 10.1109/MHS.2015.7438259

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  • Cellular Reprogramming Using Defined Factors and MicroRNAs

    Takanori Eguchi, Takuo Kuboki

    STEM CELLS INTERNATIONAL   2016 ( 2016 )   1 - 12   2016年

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    記述言語:英語   出版者・発行元:HINDAWI PUBLISHING CORP  

    Development of human bodies, organs, and tissues contains numerous steps of cellular differentiation including an initial zygote, embryonic stem (ES) cells, three germ layers, and multiple expertized lineages of cells. Induced pluripotent stem (iPS) cells have been recently developed using defined reprogramming factors such as Nanog, Klf5, Oct3/4 (Pou5f1), Sox2, and Myc. This outstanding innovation is largely changing life science and medicine. Methods of direct reprogramming of cells into myocytes, neurons, chondrocytes, and osteoblasts have been further developed using modified combination of factors such as N-myc, L-myc, Sox9, and microRNAs in defined cell/tissue culture conditions. Mesenchymal stem cells (MSCs) and dental pulp stem cells (DPSCs) are also emerging multipotent stem cells with particular microRNA expression signatures. It was shown that miRNA-720 had a role in cellular reprogramming through targeting the pluripotency factor Nanog and induction of DNA methyltransferases (DNMTs). This review reports histories, topics, and idea of cellular reprogramming.

    DOI: 10.1155/2016/7530942

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  • Changes in joint space dimension after the correction of Class II division 1 malocclusion. 国際誌

    Alberto Cacho, Tsuyoshi Ono, Takuo Kuboki, Conchita Martin

    European journal of orthodontics   37 ( 5 )   467 - 73   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    OBJECTIVE: The aim of this prospective longitudinal investigation was to compare the relationship between the temporomandibular joint (TMJ) condyles and the temporal fossae by means of tomography before and after the orthodontic correction of Class II, division 1 malocclusion using the activator appliance. MATERIALS AND METHODS: The final sample consisted of 26 consecutively treated Class II, division 1 patients (19 boys and 7 girls with an average pre-treatment age of 11 years) who underwent orthodontic treatment by means of an activator appliance. Before treatment all patients were free of signs and symptoms associated to TMJ disorders. Bilateral tomographic records before and after treatment were taken and analyzed. Outlines of the condyle and temporal fossa were automatically determined by an edge-detection protocol, and the minimum joint space dimension was automatically measured every 2 degrees. For further analysis, the joint space was divided into anterior, superior, and posterior joint spaces. RESULTS: The average treatment time with the activator appliance was 366 days. In all subjects, activator treatment resulted in a Class I dental arch relationship. After activator treatment, no differences were found in the joint space measurements in any direction. Comparisons between the right and left condyles were not significantly different. CONCLUSIONS: Joint space dimension in Class II division I children was similar before and after treatment in both TMJs.

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  • Concealed Jiggling Effect: A Novel Risk Factor for Complications of Implant-Supported Fixed Partial Denture. 国際誌

    Ryu Nakajima, Kenji Maekawa, Wataru Sonoyama, Akihiro Ueda, Takuo Kuboki

    The Journal of oral implantology   41 ( 4 )   491 - 4   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ALLEN PRESS INC  

    DOI: 10.1563/AAID-JOI-D-13-00216

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  • A risk factor analysis of accumulated postoperative pain and swelling sensation after dental implant surgery using a cellular phone-based real-time assessment.

    Ryuta Kuroi, Hajime Minakuchi, Emilio Satoshi Hara, Aya Kawakami, Kenji Maekawa, Hiroki Okada, Takuo Kuboki

    Journal of prosthodontic research   59 ( 3 )   194 - 8   2015年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: The purpose of this study was to identify the related risk factors of dental implant accumulated postoperative pain and swelling by cellular phone-based assessment. METHODS: Subjects were a consecutive series of patients who received oral implant surgery at Okayama University Hospital. Cellular phone-based questionnaire was sent at pre-set schedule to each subject every 2h on the day of surgery, and every 24h from the 2nd to 7th day post-surgery. Subjects replied in real-time the pain and swelling levels at the operated sites by an 11- and 4-grade rating-scale questionnaire. Overall intensity of individual pain and swelling was calculated by means of area under curve that drew by their time-dependent changes. Predictor variables were age, gender, presence of diabetes mellitus and/or hypertension (DM/HT), history of implant surgery, number of inserted implants, flap operation, surgical duration, pre-surgery anxiety, osteoplasty, bone quality, premedication, dosage of prescribed analgesics and local anesthesia and accumulated postoperative pain/swelling. Compliance rate and risk factors correlated with accumulated postoperative pain and swelling were calculated by multiple regression analysis. RESULTS: Final subjects were 18 females and 7 male (mean age: 59.3±7.32 yrs). Significant factors correlated with accumulated postoperative pain were DM/HT, surgical duration, premedication, bone quality, pre-surgery anxiety and postoperative swelling (R(2)=0.769, p=0.001, 0.013, 0.032, 0.007, 0.035 and 0.007, respectively). Meanwhile, significant factors associated with postoperative swelling were postoperative pain, DM/HT and bone quality (R(2)=0.365, p=0.002, 0.004, 0.008, respectively). CONCLUSION: These results suggested DM/HT and bone quality are correlated to overall intensity of postoperative pain and swelling.

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  • Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro. 国際誌

    Hiroaki Taketa, Gulsan Ara Sathi, Mahmoud Farahat, Kazi Anisur Rahman, Takayoshi Sakai, Yoshiaki Hirano, Takuo Kuboki, Yasuhiro Torii, Takuya Matsumoto

    Scientific reports   5   11468 - 11468   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In vitro fabricated biological tissue would be a valuable tool to screen newly synthesized drugs or understand the tissue development process. Several studies have attempted to fabricate biological tissue in vitro. However, controlling the growth and morphology of the fabricated tissue remains a challenge. Therefore, new techniques are required to modulate tissue growth. RGD (arginine-glycine-aspartic acid), which is an integrin-binding domain of fibronectin, has been found to enhance cell adhesion and survival; it has been used to modify substrates for in vitro cell culture studies or used as tissue engineering scaffolds. In addition, this study shows novel functions of the RGD peptide, which enhances tissue growth and modulates tissue morphology in vitro. When an isolated submandibular gland (SMG) was cultured on an RGD-modified alginate hydrogel sheet, SMG growth including bud expansion and cleft formation was dramatically enhanced. Furthermore, we prepared small RGD-modified alginate beads and placed them on the growing SMG tissue. These RGD-modified beads successfully induced cleft formation at the bead position, guiding the desired SMG morphology. Thus, this RGD-modified material might be a promising tool to modulate tissue growth and morphology in vitro for biological tissue fabrication.

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  • Interdisciplinary orthodontic treatment for a patient with generalized aggressive periodontitis: Assessment of IgG antibodies to identify type of periodontitis and correct timing of treatment. 国際誌

    Yoshihito Ishihara, Kazuya Tomikawa, Toru Deguchi, Tadashi Honjo, Koji Suzuki, Takayuki Kono, Takuo Kuboki, Hiroshi Kamioka, Shogo Takashiba, Takashi Yamashiro

    American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics   147 ( 6 )   766 - 80   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MOSBY-ELSEVIER  

    Aggressive periodontitis is a great challenge to clinicians when providing orthodontic treatment because of the potential for progression of periodontal disease. In this article, we report the successful comprehensive orthodontic treatment of bimaxillary protrusion and severe crowding in an adult with generalized aggressive periodontitis. A woman, aged 22 years 7 months, with a chief complaint of incisal crowding was diagnosed with a skeletal Class I malocclusion associated with severe anterior crowding, possibly worsened by generalized aggressive periodontitis. In addition to a periodontal examination, a blood IgG antibody titer analysis and microbiologic examination for periodontal pathogens were used to diagnose the type of periodontal disease and determine the proper timing to initiate orthodontic treatment. The total active treatment period was 28 months, followed by periodontal prostheses and regeneration therapy. Consequently, satisfactory facial profile, occlusion, and periodontal health were maintained for at least 36 months. These results indicate that efficient screening is important for providing successful orthodontic treatment in patients with advanced periodontal disease. This report also demonstrates the diagnostic importance of blood IgG antibody titer assays and microbiologic examinations to detect periodontal pathogens.

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  • In silico comparison of the reproducibility of full-arch implant provisional restorations to final restoration between a 3D Scan/CAD/CAM technique and the conventional method.

    Takuya Mino, Kenji Maekawa, Akihiro Ueda, Shizuo Higuchi, Junichi Sejima, Tetsuo Takeuchi, Emilio Satoshi Hara, Aya Kimura-Ono, Wataru Sonoyama, Takuo Kuboki

    Journal of prosthodontic research   59 ( 2 )   152 - 8   2015年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: The aim of this article was to investigate the accuracy in the reproducibility of full-arch implant provisional restorations to final restorations between a 3D Scan/CAD/CAM technique and the conventional method. METHODS: We fabricated two final restorations for rehabilitation of maxillary and mandibular complete edentulous area and performed a computer-based comparative analysis of the accuracy in the reproducibility of the provisional restoration to final restoration between a 3D scanning and CAD/CAM (Scan/CAD/CAM) technique and the conventional silicone-mold transfer technique. Final restorations fabricated either by the conventional or Scan/CAD/CAM method were successfully installed in the patient. The total concave/convex volume discrepancy observed with the Scan/CAD/CAM technique was 503.50mm(3) and 338.15 mm(3) for maxillary and mandibular implant-supported prostheses (ISPs), respectively. On the other hand, total concave/convex volume discrepancy observed with the conventional method was markedly high (1106.84 mm(3) and 771.23 mm(3) for maxillary and mandibular ISPs, respectively). CONCLUSIONS: The results of the present report suggest that Scan/CAD/CAM method enables a more precise and accurate transfer of provisional restorations to final restorations compared to the conventional method.

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  • 咬合違和感を訴える患者の実態に関する多施設実態調査 査読

    澁谷 智明, 和気 裕之, 玉置 勝司, 島田 淳, 古谷野 潔, 鱒見 進一, 窪木 拓男, 皆木 省吾, 貞森 紳丞, 矢谷 博文, 藤澤 政紀, 林 勝彦, 玉井 和樹, 成田 紀之, 原 節宏, 馬場 一美, 尾口 仁志, 金村 清孝, 山口 泰彦, 西川 洋二, 塚原 宏泰, 松香 芳三, 葉山 莉香

    日顎誌   26 ( 3 )   196 - 203   2015年2月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.11246/gakukansetsu.26.196

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  • OstemiR: A Novel Panel of MicroRNA Biomarkers in Osteoblastic and Osteocytic Differentiation from Mesencymal Stem Cells 査読

    Eguchi Takanori, Hara Emilio Satoshi, Ono Mitsuaki, Watanabe Ken, Kuboki Takuo, Calderwood Stuart

    Faseb Journal   29   2015年

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    掲載種別:研究論文(学術雑誌)  

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  • The Effect of Tooth Loss and/or Oral Function to Nutritional Status -Assessment of These Relationship

    Takeshi KIKUTANI, Mitsuyoshi YOSHIDA, Takeo SUGA, Toshihide KIMURA, Fumiyo TAMURA, Takuo KUBOKI

    34   59 - 63   2015年

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  • Food Ingestion/Mastication/Swallowing Evaluation Method Corre- sponding to the Stage of Patients and Its Application: Feasibility of an Oral Function Test

    Mami INOUE-MINAKUCHI, Kenji MAEKAWA, Takeshi KIKUTANI, Fumiyo TAMURA, Mitsuyoshi YOSHIDA, Tomohisa ONO, Yasushi TAMADA, Hisatomo KONDO, Katsuhiko KIMOTO, Shuji OHKAWA, Yuji SATO, Hiroshi SUZUKI, Misao KAWARA, Takuo KUBOKI

    34   94 - 98   2015年

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  • Topical application of lithium chloride on the pulp induces dentin regeneration. 国際誌

    Kazuya Ishimoto, Satoru Hayano, Takeshi Yanagita, Hiroshi Kurosaka, Noriaki Kawanabe, Shinsuke Itoh, Mitsuaki Ono, Takuo Kuboki, Hiroshi Kamioka, Takashi Yamashiro

    PloS one   10 ( 3 )   e0121938   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    We herein describe a novel procedure for dentin regeneration that mimics the biological processes of tooth development in nature. The canonical Wnt signaling pathway is an important regulator of the Dentin sialophosphoprotein (Dspp) expression. Our approach mimics the biological processes underlying tooth development in nature and focuses on the activation of canonical Wnt signaling to trigger the natural process of dentinogenesis. The coronal portion of the dentin and the underlying pulp was removed from the first molars. We applied lithium chloride (LiCl), an activator of canonical Wnt signaling, on the amputated pulp surface to achieve transdifferentiation toward odontoblasts from the surrounding pulpal cells. MicroCT and microscopic analyses demonstrated that the topical application of LiCl induced dentin repair, including the formation of a complete dentin bridge. LiCl-induced dentin is a tubular dentin in which the pulp cells are not embedded within the matrix, as in primary dentin. In contrast, a dentin bridge was not induced in the control group treated with pulp capping with material carriers alone, although osteodentin without tubular formation was induced at a comparatively deeper position from the pulp exposure site. We also evaluated the influence of LiCl on differentiation toward odontoblasts in vitro. In the mDP odontoblast cell line, LiCl activated the mRNA expression of Dspp, Axin2 and Kallikrein 4 (Klk4) and downregulated the Osteopontin (Osp) expression. These results provide a scientific basis for the biomimetic regeneration of dentin using LiCl as a new capping material to activate dentine regeneration.

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  • 睡眠時ブラキシズムの簡便な診断法の確立と対処法の検討 査読

    大倉一夫, 大川周治, 藤澤政紀, 櫻井薫, 馬場一美, 小川匠, 矢谷博文, 窪木拓男, 松香芳三

    日本歯科医学会雑誌   34   79 - 83   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • BMP2アンタゴニストを阻害するL51Pは骨形成を促進する 国際誌

    Bone   69   165 - 73   2014年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and control.

    Hajime Minakuchi, Chiharu Sogawa, Emilio Satoshi Hara, Haruna Miki, Kenji Maekawa, Norio Sogawa, Shigeo Kitayama, Yoshizo Matsuka, Glenn T Clark, Takuo Kuboki

    Journal of prosthodontic research   58 ( 4 )   217 - 22   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: The aim of this study was to evaluate the correlation between sleep bruxism (SB) frequency and serotonin transporter (SERT)-driven serotonin (5-HT)-uptake in platelets. METHODS: Subjects were dental trainee residents and faculty members of Okayama University Hospital who were aware of having severe or no SB. SB frequency was assessed for 3-consecutive nights by a self-contained electromyographic detector/analyzer, which indicated individual SB levels as one of four grades (score 0, 1, 2 and 3). Subjects were classified as normal control (NC) when SB scores indicated only 0 or 1 during the 3 nights, or as severe SB for scores 2 or 3. Those subjects whose scores fluctuated from 0 to 3 during the 3 nights were omitted from further analysis. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. Amounts of SERTs proteins collected from peripheral platelets were quantified using ELISA, and SERTs transport activity was assessed by uptake assay using [3H]-5-HT. RESULTS: Thirteen severe SB subjects and 7 NC subjects were eligible. Gender distribution, mean age, 5-HT concentration and total amounts of SERT protein in platelets showed no significant differences between NC and severe SB (p=0.85: Chi-squared test; p=0.64, 0.26, 0.46: t-test). However, [3H]-5-HT uptake by platelets was significantly greater in NC compared to severe SB subjects (12.79±1.97, 8.27±1.91 fmol/10(5) platelets/min, p<0.001, t-test). CONCLUSION: The results of this pilot study suggest a possible correlation between peripheral platelet serotonin transporter uptake ability and SB severity.

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  • Dentin-smear remains at self-etch adhesive interface. 国際誌

    Atsushi Mine, Jan De Munck, Marcio Vivan Cardoso, Kirsten L Van Landuyt, André Poitevin, Annelies Van Ende, Mariko Matsumoto, Yasuhiro Yoshida, Takuo Kuboki, Hirofumi Yatani, Bart Van Meerbeek

    Dental materials : official publication of the Academy of Dental Materials   30 ( 10 )   1147 - 53   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    OBJECTIVE: The bonding potential of 'mild' self-etch adhesives may be compromised due to smear interference, as they may not dissolve/penetrate the smear layer effectively due to their relatively low acidity. We observed that the thickness of the dentin smear layer differed depending on the surface-preparation methodology used. METHODS: The interaction of an (ultra-)mild self-etch adhesive (Clearfil S3 Bond, Kuraray Noritake) with human dentin, prepared either using a medium-grit diamond bur ('thick', clinically relevant smear layer) or 600-grit SiC-paper ('thin' smear layer), or just fractured (smear-free), was evaluated using high-resolution transmission electron microscopy (TEM). Non-demineralized/demineralized 30-100nm interfacial cross-sections were prepared following common TEM-specimen processing and diamond-knife ultra-microtomy. RESULTS: The adhesive did not dissolve the bur-cut, nor the SiC-ground smear layer, but impregnated it. Within this 'resin-smear complex', hydroxyapatite was abundantly present. At fractured dentin, this complex was not present, while the actual layer of interaction of the adhesive was limited to about 100nm. Non-demineralized 'ultra-thin' (30-50nm) sections confirmed the interfacial ultra-structure to differ for the three surface-preparation methods. An electron dense band was consistently disclosed at the adhesive interface, most likely representing the documented chemical interaction of the functional monomer 10-MDP with Ca. SIGNIFICANCE: The dentin surface-preparation method significantly affects the nature of the smear layer and the interaction with the ultra-mild self-etch adhesive.

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  • 完全な臓器としての歯根膜付きインプラントの開発成功 国際誌

    Scientific Reports   4   6044 - 6044   2014年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep06044

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  • Direct interaction between CCN family protein 2 and fibroblast growth factor 1. 国際誌

    Tarek Abd El Kader, Satoshi Kubota, Ken Anno, Saho Tanaka, Takashi Nishida, Takayuki Furumatsu, Eriko Aoyama, Takuo Kuboki, Masaharu Takigawa

    Journal of cell communication and signaling   8 ( 2 )   157 - 63   2014年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    In an attempt to find out a new molecular counterpart of CCN family protein 2 (CCN2), a matricellular protein with multiple functions, we performed an interactome analysis and found fibroblast growth factor (FGF) -1 as one of the candidates. Solid-phase binding assay indicated specific binding between CCN2 and FGF-1. This binding was also confirmed by surface plasmon resonance (SPR) analysis that revealed a dissociation constant (Kd) of 3.98 nM indicating strong molecular interaction between the two. RNA analysis suggested that both FGF-1 and CCN2 could be produced by chondrocytes and thus their interaction in the cartilage is possible. These findings for the first time indicate the direct interaction of CCN2 and FGF-1 and suggest the co-presence of these molecules in the cartilage microenvironment. CCN2 is a well-known promoter of cartilage development and regeneration, whereas the physiological and pathological role of FGF-1 in cartilage mostly remains unclear. Biological role of FGF-1 itself in cartilage is also suspected.

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  • A short-term treatment with tumor necrosis factor-alpha enhances stem cell phenotype of human dental pulp cells. 国際誌

    Mayu Ueda, Takuo Fujisawa, Mitsuaki Ono, Emilio Satoshi Hara, Hai Thanh Pham, Ryu Nakajima, Wataru Sonoyama, Takuo Kuboki

    Stem cell research & therapy   5 ( 1 )   31 - 31   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    INTRODUCTION: During normal pulp tissue healing, inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) or interleukins, act in the initial 48 hours (inflammatory phase) and play important roles not only as chemo-attractants of inflammatory cells and stem/progenitor cells but also in inducing a cascade of reactions toward tissue regeneration or reparative dentin formation or both. Previous reports have shown that inflammatory cytokines regulate the differentiation capacity of dental pulp stem/progenitor cells (DPCs), but none has interrogated the impact of these cytokines on the stem cell phenotype of stem/progenitor cells. This study investigated the effects of a short-term treatment with TNF-α on the stem cell phenotype and differentiation ability of human DPCs. METHODS: An in vivo mouse model of pulp exposure was performed for analysis of expression of the mesenchymal stem cell marker CD146 in DPCs during the initial stage of inflammatory response. For in vitro studies, human DPCs were isolated and incubated with TNF-α for 2 days and passaged to eliminate TNF-α completely. Analysis of stem cell phenotype was performed by quantification of cells positive for mesenchymal stem cell markers SSEA-4 (stage-specific embryonic antigen 4) and CD146 by flow cytometry as well as by quantitative analysis of telomerase activity and mRNA levels of OCT-4 and NANOG. Cell migration, colony-forming ability, and differentiation toward odontogenesis and adipogenesis were also investigated. RESULTS: The pulp exposure model revealed a strong staining for CD146 during the initial inflammatory response, at 2 days after pulp exposure. In vitro experiments demonstrated that a short-term (2-day) treatment of TNF-α increased by twofold the percentage of SSEA-4+ cells. Accordingly, STRO-1, CD146, and SSEA-4 protein levels as well as OCT-4 and NANOG mRNA levels were also significantly upregulated upon TNF-α treatment. A short-term TNF-α treatment also enhanced DPC function, including the ability to form cell colonies, to migrate, and to differentiate into odontogenic and adipogenic lineages. CONCLUSIONS: A short-term treatment with TNF-α enhanced the stem cell phenotype, migration, and differentiation ability of DPCs.

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  • miRNA-720 regulates the stem cell phenotype and differentiation of human dental pulp-derived mesenchymal stromal cells 査読

    Hara Emilio Satoshi, Ono Mitsuaki, Eguchi Takanori, Hai Thanh Pham, Tajima Shoji, Calderwood Stuart K, Matsumoto Takuya, Kuboki Takuo, IEEE

    2014 International Symposium on Micro-Nanomechatronics and Human Science (Mhs)   2014年

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1109/MHS.2014.7006080

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  • 抜歯窩から採取した未分化間葉系幹細胞

    Journal of Dental Research   93 ( 11 )   1133 - 1140   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/0022034514549377

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  • Efficient bone formation in swine socket-lift model using E. coli-derived rhBMP-2 adsorbed in b-TCP.

    Ono M, Sonoyama W, Yamamoto K, Oida Y, Akiyama K, Shinkawa S, Nakajima R, Pham HT, Hara ES, Kuboki T

    199 ( 4 )   249 - 255   2014年

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  • Efficient bone formation in a swine socket lift model using Escherichia coli-derived recombinant human bone morphogenetic protein-2 adsorbed in β-tricalcium phosphate. 査読 国際誌

    Ono M, Sonoyama W, Yamamoto K, Oida Y, Akiyama K, Shinkawa S, Nakajima R, Pham HT, Hara ES, Kuboki T

    Cells, tissues, organs   199 ( 4 )   249 - 55   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1159/000369061

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  • Immunotherapy in Transplantation 査読

    Kentaro Akiyama, Emilio Hara Satoshi, Takuo Kuboki

    Stem Cell Biology and Tissue Engineering in Dental Sciences   831   2014年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier  

    DOI: 10.1016/B978-0-12-397157-9.00068-0

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  • Hydrolytic stability of three-step etch-and-rinse adhesives in occlusal class-I cavities. 査読 国際誌

    Jan De Munck, Atsushi Mine, Marcio Vivan Cardoso, Kirsten L Van Landuyt, Anne-Katrin Lührs, André Poitevin, Masao Hanabusa, Takuo Kuboki, Bart Van Meerbeek

    Clinical oral investigations   17 ( 8 )   1911 - 8   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:8  

    OBJECTIVES: A dental adhesive without small and hydrophilic monomers such as 2-hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) would be beneficial in order to avoid contact allergies. However, these monomers are important to increase infiltration and polymerization of the adhesive. Therefore, the purpose of this study was to evaluate the bonding effectiveness and bond durability of a more hydrophobic and biocompatible adhesive as compared to a conventional three-step etch-and-rinse adhesive. METHODS: Sixteen non-carious human third molars were used to determine the micro-tensile bond strength testing (μTBS) and interfacial ultrastructure by transmission electron microscopy (TEM) of the more hydrophobic cmf adhesive system (Saremco) adhesive as compared to the control OptiBond FL (Kerr). RESULTS: The more hydrophobic and biocompatible three-step etch-and-rinse adhesive was able to produce a reasonable short-time bonding effectiveness. In the long term, the collagen fibrils in the hybrid layer were not effectively protected and were prone to hydrolytic degradation. As a result, long-term bonding effectiveness of this novel adhesive was very low. CONCLUSIONS: Application of a more hydrophobic adhesive without altering the application procedure considerably results in a reduced durability of the created bond CLINICAL RELEVANCE: Omitting small and hydrophilic components from the adhesive formulation may impair the durability of your composite restoration.

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  • 補綴歯科治療病名システムの信頼性と妥当性の検討 査読

    松香 芳三, 萩原 芳幸, 玉置 勝司, 竹内 久裕, 藤澤 政紀, 小野 高裕, 築山 能大, 永尾 寛, 津賀 一弘, 會田 英紀, 近藤 尚知, 笛木 賢治, 塚崎 弘明, 石橋 寛二, 藤井 重壽, 平井 敏博, 佐々木 啓一, 矢谷 博文, 五十嵐 順正, 佐藤 裕二, 市川 哲雄, 松村 英雄, 山森 徹雄, 窪木 拓男, 馬場 一美, 古谷野 潔

    日補綴会誌   5 ( 3 )   281 - 290   2013年11月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2186/ajps.5.281

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  • Regulation of CCN2 gene expression and possible roles in developing tooth germs. 査読 国際誌

    Manabu Kanyama, Tsuyoshi Shimo, Hiroki Sugito, Motohiko Nagayama, Takuo Kuboki, Maurizio Pacifici, Eiki Koyama

    Archives of oral biology   58 ( 11 )   1659 - 66   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    CCN proteins are extracellular and cell-associated molecules involved in several developmental processes, but their expression patterns and regulation in tooth development remain unclear. Here we first determined the expression patterns of CCN genes in mouse tooth germs. We found that at early stages CCN2 was detected in dental lamina, dental mesenchyme, and primary enamel knot, while other CCN family members were expressed broadly. By the bell stage, all members were expressed in differentiating odontoblasts and ameloblasts, but CCN1 and CCN2 transcripts were conspicuous in differentiating osteoblasts in dental follicle. Next, we asked what signalling molecules regulate CCN2 expression and what roles CCN2 may have. We found that upon surgical removal of dental epithelium CCN2 was not longer expressed in dental mesenchyme in cultured bud stage germs. Implantation of beads pre-coated with BMPs and FGFs onto E12-13 mandibular explants induced CCN2 expression in dental mesenchyme. There was a dose-dependent effect of BMP-4 on CCN2 induction; a concentration of 100 ng/μl was able to induce strong CCN2 expression while a minimum concentration of 25 ng/μl was needed to elicit appreciable expression. Importantly, Noggin treatment inhibited endogenous and BMP-induced CCN2 expression, verifying that CCN2 expression in developing tooth germs requires BMP signalling. Lastly, we found that rCCN2 stimulated proliferation in dental mesenchyme in a dose-dependent manner. Together, the data indicate that expression of CCN genes is spatio-temporally regulated in developing tooth germs. CCN2 expression appears to depend on epithelial and mesenchymal-derived signalling factors, and CCN2 can elicit strong proliferation in dental mesenchyme.

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  • 固定性架工義歯学技工実習での視覚教材としての動画配信システム 査読

    中島 隆, 水口 一, 新川重彦, 瀧内博也, 正木明日香, 三野卓哉, 笈田育尚, 木村 彩, 前川賢治, 窪木拓男

    岡山歯学会雑誌   32 ( 1 )   17 - 23   2013年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

    Web媒体のデモンストレーション動画配信システム(Web Class)を歯学部学生の固定性架工義歯学基礎実習に導入し、教育効果に与える影響について検討した。対象はWeb Class導入前の歯学部4年次学生59名と導入後の65名である。実習期間にWeb Classを利用した総回数は1132回、総利用時間は207時間28分で、1人当りの平均利用時間は3時間12分であった。Web Class導入前後で、学生の技能習得の向上が認められた。導入後のアンケートにおいて、Web Classは実習を行う上で非常に有益、いくらか有益と回答したのはそれぞれ69%、31%で、予習に活用できた、イメージトレーニングやシミュレーションをするのに役立った、などの意見があった。

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  • Anti-fibrotic effect of CCN3 accompanied by altered gene expression profile of the CCN family. 査読 国際誌

    Tarek Abd El Kader, Satoshi Kubota, Danilo Janune, Takashi Nishida, Takako Hattori, Eriko Aoyama, Bernard Perbal, Takuo Kuboki, Masaharu Takigawa

    Journal of cell communication and signaling   7 ( 1 )   11 - 8   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    CCN family proteins 2 and 3 (CCN2 and CCN3) belong to the CCN family of proteins, all having a high level of structural similarity. It is widely known that CCN2 is a profibrotic molecule that mediates the development of fibrotic disorders in many different tissues and organs. In contrast, CCN3 has been recently suggested to act as an anti-fibrotic factor in several tissues. This CCN3 action was shown earlier to be exerted by the repression of the CCN2 gene expression in kidney tissue, whereas different findings were obtained for liver cells. Thus, the molecular action of CCN3 yielding its anti-fibrotic effect is still controversial. Here, using a general model of fibrosis, we evaluated the effect of CCN3 overexpression on the gene expression of all of the CCN family members, as well as on that of fibrotic marker genes. As a result, repression of CCN2 gene expression was modest, while type I collagen and α-smooth muscle actin gene expression was prominently repressed. Interestingly, not only CCN2, but also CCN4 gene expression showed a decrease upon CCN3 overexpression. These findings indicate that fibrotic gene induction is under the control of a complex molecular network conducted by CCN family members functioning together.

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  • Novel chondrogenic and chondroprotective effects of the natural compound harmine. 査読 国際誌

    Emilio Satoshi Hara, Mitsuaki Ono, Satoshi Kubota, Wataru Sonoyama, Yasutaka Oida, Takako Hattori, Takashi Nishida, Takayuki Furumatsu, Toshifumi Ozaki, Masaharu Takigawa, Takuo Kuboki

    Biochimie   95 ( 2 )   374 - 81   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A significant number of natural compounds have been shown to regulate the behavior of the cells, in collaboration with cellular proteins. CCN2/connective tissue growth factor (CTGF) has been reported to have essential roles in cartilage development, chondrocyte proliferation and differentiation as well as regulation of the extracellular matrix metabolism. Previous studies demonstrated the capability of CCN2 to regenerate surgical defects in articular cartilage of rat knee. Also, transgenic mice over-expressing cartilage-specific CCN2 were shown to be more resistant to aging-related cartilage degradation. We hypothesized that small molecules that induce CCN2 in chondrocytes could be novel candidates to increase the resistance to aging-related cartilage degradation, or even to correct cartilage degenerative changes incurred in OA. Therefore, this study screened a compound library and identified the β-carboline alkaloid harmine as a novel inducer of CCN2 in human chondrocytic HCS-2/8 cells and osteoarthritic articular chondrocytes. Harmine increased the expression of the cartilage markers aggrecan and COL2α1, as well as that of the master regulator of chondrogenesis, SOX-9. Moreover, harmine notably induced chondrogenesis of prechondrocytic ATDC5 cells in micromass cultures. The chondroprotective effect of harmine was investigated under inflammatory condition by stimulation with TNFα, and harmine was shown to ameliorate TNFα-induced decrease in expression of CCN2 and cartilage markers. These findings uncover novel chondrogenic effects of harmine and indicate harmine as a potential drug for prevention and/or repair of cartilage degradation.

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  • The CCN2-inducer harmine promotes chondrogenesis and protects against TNFα-induced ablation of chondrocytic phenotype 査読

    Hara, E.S, M. Ono, S. Kubota, W. Sonoyama, Y. Oida, T. Hattori, T. Nishida, T. Furumatsu, T. Ozaki, M. Takigawa, T. Kuboki

    Biochimie   95   374 - 381   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Regeneration of calvarial defects with Escherichia coli -derived rhBMP-2 adsorbed in PLGA membrane. 査読 国際誌

    Mitsuaki Ono, Wataru Sonoyama, Kazuki Nema, Emilio Satoshi Hara, Yasutaka Oida, Hai Thanh Pham, Katushi Yamamoto, Kazuo Hirota, Kazushige Sugama, Walter Sebald, Takuo Kuboki

    Cells, tissues, organs   198 ( 5 )   367 - 76   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: Escherichia coli-derived recombinant human bone morphogenetic protein-2 (E-BMP-2) has been shown to be as effective as mammalian cell-derived BMP-2. However, several in vitro and in vivo experiments are still necessary to validate the effectiveness of E-BMP-2 due to the difference in synthesis process, mainly related to protein nonglycosylation. The objective of this study was to investigate whether biodegradable polylactide-co-glycolide (PLGA) membrane is a suitable carrier for E-BMP-2 delivery for bone regeneration of critical-sized defects in rat calvaria. MATERIALS AND METHODS: First, the osteoinductive effect of E-BMP-2 was confirmed in vitro in mouse bone marrow stromal cells by analysis of osteocalcin mRNA levels, and calcium deposition was detected by alizarin red staining. Before in vivo experiments, the release profile of E-BMP-2 from PLGA membranes was determined by ELISA. E-BMP-2 (0, 1, 5 and 10 μg/μl) was applied for ectopic and orthotopic bone formation and was analyzed by X-ray, micro-CT and histology. RESULTS: Release-profile testing showed that PLGA membrane could retain 94% of the initially applied E-BMP-2. Ectopic bone formation assay revealed that combination of E-BMP-2/PLGA membrane strongly induced bone formation. Stronger osteoinductivity with complete repair of critical-sized defects was observed only with PLGA membranes adsorbed with 5 and 10 μg/μl of E-BMP-2, whereas no bone formation was observed in the groups that received no membrane or 0-μg/μl dose of E-BMP-2. CONCLUSION: PLGA membrane was shown to be a suitable carrier for sustained release of E-BMP-2, and the E-BMP-2/PLGA membrane combination was demonstrated to be efficient in bone regeneration in a model of critical-sized defects.

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  • Multiple sleep bruxism data collected using a self-contained EMG detector/analyzer system in asymptomatic healthy subjects 査読

    Hajime Minakuchi, Chiyomi Sakaguchi, Emilio S. Hara, Kenji Maekawa, Yoshizo Matsuka, Glenn T. Clark, Takuo Kuboki

    SLEEP AND BREATHING   16 ( 4 )   1069 - 1072   2012年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER HEIDELBERG  

    Small, self-contained electromyographic (EMG) detector/analyzer (D/A) devices have become available for the detection of jaw muscle activity events above threshold. These devices claim to be less intrusive to the subjects sleep so it is less prone to induce disturbed sleep. The objective of this study was to evaluate for night-to-night variability and examine for a systematic alteration on the first night in EMG levels.
    Ten asymptomatic healthy volunteers (mean age, 26.8 +/- 3.78) were recorded for six sequential nights in their home environment using EMG D/A system. The device yields a nightly EMG level above threshold score on a 0-4 level. Because the data are categorical and nonparametric, the data of the ten subjects across six nights were submitted to a Friedman repeated measures ANOVA. The significant level was set as alpha equal to 0.05.
    The median and mode values of the subjects were tabulated and analyzed and we did not find a significant difference in EMG D/A level across the six nights (p = 0.287, Kendall's coefficient of concordance = 0.124, Friedman two-way repeated measures ANOVA). The data did show clear and substantial night-to-night variability.
    Substantial night-to-night variability in masseter EMG activity levels was clearly observed in our subjects. There was no evidence of a suppressed or elevated first-night effect-like variability on masseter muscle EMG level seen in these subjects using a small portable self-contained EMG detector/analyzer. These data suggest that recordings should be at least 5-6-nights duration to establish a reasonable measure of an individual's average nightly masseter EMG level.

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  • A problem-based learning tutorial for dental students regarding elderly residents in a nursing home in Japan. 査読 国際誌

    Yoshizo Matsuka, Ryu Nakajima, Haruna Miki, Aya Kimura, Manabu Kanyama, Hajime Minakuchi, Shigehiko Shinkawa, Hiroya Takiuchi, Kumiko Nawachi, Kenji Maekawa, Hikaru Arakawa, Takuo Fujisawa, Wataru Sonoyama, Atsushi Mine, Emilio Satoshi Hara, Takeshi Kikutani, Takuo Kuboki

    Journal of dental education   76 ( 12 )   1580 - 8   2012年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:12  

    This educational trial was an eight-day problem-based learning (PBL) course for fourth-year predoctoral students at Okayama University's dental school who interviewed elderly residents living in a nursing home. The purpose of this PBL course was to introduce geriatric dentistry to the students by allowing them, independently, to discover the clinical problems of elderly patients as well as the solutions. The sixty-five students were divided into nine small groups and received patient information (age, gender, degree of care needed, medical history, food type, medications, and oral condition) in datasheets before visiting the nursing home. Each group of students directly interviewed one patient and the caregivers and identified the patient's medical, psychological, and social problems. After the interview, the students participated in a PBL tutorial to delineate a management approach for the patient's problems. To measure the efficacy of this program, the students completed a questionnaire before and after the course regarding their level of understanding of and attitudes toward geriatric dentistry, clinical research, and self-study. The results showed that student's perceptions of their knowledge about and attitudes toward oral health care for the elderly significantly increased after the PBL course, which suggests that such tutorials should be an option for dental curricula.

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  • Multiple sleep bruxism data collected using a self-contained EMG detector/analyzer system in asymptomatic healthy subjects. 査読 国際誌

    Hajime Minakuchi, Chiyomi Sakaguchi, Emilio S Hara, Kenji Maekawa, Yoshizo Matsuka, Glenn T Clark, Takuo Kuboki

    Sleep & breathing = Schlaf & Atmung   16 ( 4 )   1069 - 72   2012年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: Small, self-contained electromyographic (EMG) detector/analyzer (D/A) devices have become available for the detection of jaw muscle activity events above threshold. These devices claim to be less intrusive to the subjects sleep so it is less prone to induce disturbed sleep. The objective of this study was to evaluate for night-to-night variability and examine for a systematic alteration on the first night in EMG levels. METHODS: Ten asymptomatic healthy volunteers (mean age, 26.8 ± 3.78) were recorded for six sequential nights in their home environment using EMG D/A system. The device yields a nightly EMG level above threshold score on a 0-4 level. Because the data are categorical and nonparametric, the data of the ten subjects across six nights were submitted to a Friedman repeated measures ANOVA. The significant level was set as alpha equal to 0.05. RESULTS: The median and mode values of the subjects were tabulated and analyzed and we did not find a significant difference in EMG D/A level across the six nights (p = 0.287, Kendall's coefficient of concordance = 0.124, Friedman two-way repeated measures ANOVA). The data did show clear and substantial night-to-night variability. CONCLUSION: Substantial night-to-night variability in masseter EMG activity levels was clearly observed in our subjects. There was no evidence of a suppressed or elevated first-night effect-like variability on masseter muscle EMG level seen in these subjects using a small portable self-contained EMG detector/analyzer. These data suggest that recordings should be at least 5-6-nights duration to establish a reasonable measure of an individual's average nightly masseter EMG level.

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  • A prosthodontic medicine with collaboration between medical and dental sciences. 査読

    Takuo Kuboki

    Journal of prosthodontic research   56 ( 4 )   227 - 8   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.jpor.2012.10.002

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  • Matrix metalloproteinase-8 is the major potential collagenase in active peri-implantitis. 査読

    Hikaru Arakawa, Junji Uehara, Emilio Satoshi Hara, Wataru Sonoyama, Aya Kimura, Manabu Kanyama, Yoshizo Matsuka, Takuo Kuboki

    Journal of prosthodontic research   56 ( 4 )   249 - 55   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: Current clinical procedures to control or regenerate bone loss due to peri-implantitis are not predictable neither accomplish complete resolution. Therefore, early detection of the onset and the active periods of bone loss are crucial for prevention of extensive peri-implant bone resorption. This study aimed to determine a possible association between the presence of collagenases (MMP-1, MMP-8 and MMP-13) in peri-implant sulcular fluid (PISF) and active periods of bone loss by annually adjusted vertical bone loss (AVBL) measurements. METHODS: Intended sample consisted of 76 consecutive patients who received oral implant treatment at the Fixed Prosthodontic Clinic of Okayama University Hospital from 1990 to 2000. Twelve subjects were lost to follow-up or refused to participate. Consequently, the actual sample consisted of 64 patients who were followed-up for at least one year. Those patients with AVBL>0.6mm were included in the severe peri-implantitis group, and randomly selected, age-, gender- and implantation site-matched healthy patients (AVBL<0.3mm) comprised the control group. PISF samples were collected from both groups and further analyzed by western blot for detection of collagenases. RESULTS: Four patients presented severe peri-implantitis. MMP-8 was the only collagenase detected in peri-implant sites with ongoing bone loss. PISF samples from control group showed no positive reactions to any collagenase. CONCLUSION: This study showed MMP-8 as a possible marker for progressive bone loss in peri-implantitis.

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  • Matrix metalloproteinase-8 is the major potential collagenase in active peri-implantitis 査読

    Hikaru Arakawa, Junji Uehara, Emilio Satoshi Hara, Wataru Sonoyama, Aya Kimura, Manabu Kanyama, Yoshizo Matsuka, Takuo Kuboki

    JOURNAL OF PROSTHODONTIC RESEARCH   56 ( 4 )   249 - 255   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Purpose: Current clinical procedures to control or regenerate bone loss due to peri-implantitis are not predictable neither accomplish complete resolution. Therefore, early detection of the onset and the active periods of bone loss are crucial for prevention of extensive pen-implant bone resorption. This study aimed to determine a possible association between the presence of collagenases (MMP-1, MMP-8 and MMP-13) in peri-implant sulcular fluid (PISF) and active periods of bone loss by annually adjusted vertical bone loss (AVBL) measurements.
    Methods: Intended sample consisted of 76 consecutive patients who received oral implant treatment at the Fixed Prosthodontic Clinic of Okayama University Hospital from 1990 to 2000. Twelve subjects were lost to follow-up or refused to participate. Consequently, the actual sample consisted of 64 patients who were followed-up for at least one year. Those patients with AVBL &gt; 0.6 mm were included in the severe peri-implantitis group, and randomly selected, age-, gender- and implantation site-matched healthy patients (AVBL &lt;0.3 mm) comprised the control group. PISF samples were collected from both groups and further analyzed by western blot for detection of collagenases.
    Results: Four patients presented severe peri-implantitis. MMP-8 was the only collagenase detected in pen-implant sites with ongoing bone loss. PISF samples from control group showed no positive reactions to any collagenase.
    Conclusion: This study showed MMP-8 as a possible marker for progressive bone loss in peri-implantitis. (C) 2012 Japan Prosthodontic Society. Published by Elsevier Ireland. All rights reserved.

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  • Identification of novel transcription-regulating genes expressed during murine molar development. 査読 国際誌

    Kenta Uchibe, Hirohito Shimizu, Shigetoshi Yokoyama, Takuo Kuboki, Hiroshi Asahara

    Developmental dynamics : an official publication of the American Association of Anatomists   241 ( 7 )   1217 - 26   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: The mechanism of tooth development is a complex process regulated by numerous genes including transcription factors, growth factors, and other intra- and extracellular molecules. Especially, transcription factors play a central role in gene expression, regulating a wide spectrum of biological processes including organogenesis. Substantial evidence has been demonstrated by a number of studies using genetically engineered animal models. However, detailed molecular mechanisms of tooth development have not been completely elucidated, partially because numerous genes that play essential roles in tooth development remain unidentified. RESULTS: In this study, we conducted an expression-based screening using gene expression database and in situ hybridization assays. Based on the gene expression database "EMBRYS," 207 out of 1,520 genes were expressed in the maxillary and/or mandibular processes and thus were selected for further analysis by section in situ hybridization. Among these candidates, 28 genes were newly identified as potential factors associated with tooth development by in situ hybridization assays with frontal sections of embryonic day 13.5 and 14.5 mouse embryos. The expression patterns were also examined at embryonic day 16.5 and 18.5. CONCLUSIONS: These results will contribute to elucidating the mechanisms of tooth development and to improving the technology for regeneration of tooth.

    DOI: 10.1002/dvdy.23808

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  • Bonding effectiveness of a new 'multi-mode' adhesive to enamel and dentine. 査読 国際誌

    Masao Hanabusa, Atsushi Mine, Takuo Kuboki, Yasuko Momoi, Annelies Van Ende, Bart Van Meerbeek, Jan De Munck

    Journal of dentistry   40 ( 6 )   475 - 84   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:6  

    OBJECTIVES: Self-etch adhesives are well adopted in general practice, obviously primarily thanks to their ease of use and fast application time. Nevertheless, phosphoric acid is still often recommended to beforehand etch enamel following a so-called 'selective' enamel-etch technique, this in particular when most cavity margins end in enamel. The purpose of this study was to test if a new one-step adhesive can be applied in a multi-mode manner, this following different, either 'full' or 'selective', self-etch and etch-and-rinse approaches. Specific research hypotheses tested were that prior phosphoric-acid etching did not affect the bonding effectiveness of the one-step adhesive to enamel and dentine, and that the bonding effectiveness to dentine was also not affected when the adhesive was applied either following a 'dry-bonding' or 'wet-bonding' etch-and-rinse technique. METHODS: The micro-tensile bond strength (μTBS) of the one-step self-etch adhesive G-Bond Plus (GC, Tokyo, Japan; 1-SEA) was measured when it was bonded to bur-cut enamel following either a 'self-etch' or an 'etch-and-rinse' adhesive protocol, and to bur-cut dentine when applied following either a 'self-etch', a 'dry-bonding' or a 'wet-bonding' etch-and-rinse adhesive protocol. Bond-strength testing was corroborated by ultra-structural analysis of the interfacial interaction at enamel and dentine using transmission electron microscopy (TEM). RESULTS: Prior phosphoric-acid etching significantly increased the bonding effectiveness of the 1-SEA to enamel. A clearly enhanced micro-retentive surface was revealed by TEM. To dentine, no statistically significant difference in bonding effectiveness was recorded when the 1-SEA was either applied following a self-etch or both etch-and-rinse approaches. The 'dry-bonding' etch-and-rinse protocol was significantly more effective than its 'wet-bonding' version. TEM however revealed indications of low-quality hybridisation following both etch-and-rinse approaches, in particular in the form of a porous and poorly resin-infiltrated collagen mesh. CONCLUSIONS: While phosphoric-acid etching definitely improved bonding of the one-step self-etch adhesive to enamel, one should be more careful with additional phosphoric-acid etching of dentine. Although the bond strength was not reduced, the resultant adhesive interface appeared ultra-structurally more vulnerable to biodegradation.

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  • Videoendoscopic assessment of swallowing function to predict the future incidence of pneumonia of the elderly 査読

    N. Takahashi, T. Kikutani, F. Tamura, M. Groher, T. Kuboki

    Journal of Oral Rehabilitation   39 ( 6 )   429 - 437   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of the present study was to examine what dysphagic signs identified by videoendoscopy (VE) could predict the incidence of pneumonia and body weight loss in elderly patients living in nursing homes. This study was performed at six nursing care facilities in Japan from March 2007 to February 2009. The 148 subjects (85·1±8·0years, male/female: 43/105) were evaluated for their feeding and swallowing movements by clinical and VE examinations during the consumption of a regular meal. The VE examination items included the existence/absence of pharyngeal residue, laryngeal penetration, and aspiration of food and saliva. The patients were followed-up for 3months with individualized feeding therapy based on the results of the clinical/VE examination at baseline, and the incidence of pneumonia was examined as the primary outcome. In patients without pneumonia, the body weight change was also measured as a secondary outcome. The risk factors for pneumonia and body weight loss (of 3% or more) were identified among the clinical/VE examination items by a Cox proportional hazard analysis. Even with elaborative feeding therapy, 12 (8·1%) of the 148 patients developed pneumonia during the 3months follow-up period. The existence of signs of 'silent aspiration of saliva' or 'aspiration of saliva' detected by VE examination was a significant risk factor for both pneumonia and a body weight loss of 3% or more. This study shows that 'aspiration of saliva' detected by VE is a significant risk factor for both pneumonia and body weight loss in elderly patients living in nursing homes. © 2012 Blackwell Publishing Ltd.

    DOI: 10.1111/j.1365-2842.2011.02286.x

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  • 施設に入所している要介護高齢者の問題点を抽出するテュートリアル演習 査読

    松香 芳三, 縄稚 久美子, 中島 隆, 三木 春奈, 木村 彩, 完山 学, 水口 一, 新川 重彦, 瀧内 博也, 前川 賢治, 荒川 光, 藤澤 拓生, 園山 亘, 峯 篤史, 菊谷 武, 窪木 拓男

    日本補綴歯科学会誌   4 ( 特別号 )   279 - 279   2012年5月

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    記述言語:日本語   出版者・発行元:(公社)日本補綴歯科学会  

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  • Stage-specific embryonic antigen-4 identifies human dental pulp stem cells. 査読 国際誌

    Noriaki Kawanabe, Satoko Murata, Hiroaki Fukushima, Yoshihito Ishihara, Takeshi Yanagita, Emmy Yanagita, Mitsuaki Ono, Hiroshi Kurosaka, Hiroshi Kamioka, Tomoo Itoh, Takuo Kuboki, Takashi Yamashiro

    Experimental cell research   318 ( 5 )   453 - 63   2012年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Embryonic stem cell-associated antigens are expressed in a variety of adult stem cells as well as embryonic stem cells. In the present study, we investigated whether stage-specific embryonic antigen (SSEA)-4 can be used to isolate dental pulp (DP) stem cells. DP cells showed plastic adherence, specific surface antigen expression, and multipotent differentiation potential, similar to mesenchymal stem cells (MSC). SSEA-4+ cells were found in cultured DP cells in vitro as well as in DP tissue in vivo. Flow cytometric analysis demonstrated that 45.5% of the DP cells were SSEA-4+. When the DP cells were cultured in the presence of all-trans-retinoic acid, marked downregulation of SSEA-3 and SSEA-4 and the upregulation of SSEA-1 were observed. SSEA-4+ DP cells showed a greater telomere length and a higher growth rate compared to ungated and SSEA-4- cells. A clonal assay demonstrated that 65.5% of the SSEA-4+ DP cells had osteogenic potential, and the SSEA-4+ clonal DP cells showed multilineage differentiation potential toward osteoblasts, chondrocytes, and neurons in vitro. In addition, the SSEA-4+ DP cells had the capacity to form ectopic bone in vivo. Thus, our results suggest that SSEA-4 is a specific cell surface antigen that can be used to identify DP stem cells.

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  • Influence of resin coating materials on Porphyromonas gingivalis attachment. 査読

    Ai Kumada, Yoshizo Matsuka, Atsushi Mine, Mitsuaki Ono, Junji Uehara, Norihiro Sonoi, Takashi Ito, Shogo Takashiba, Takuo Kuboki

    Dental materials journal   31 ( 1 )   86 - 91   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:1  

    Resin coating materials have been used for composite resin or provisional restoration in order to prevent plaque accumulation on their surfaces. However, it is not clear whether the coating materials influence attachment of periodontal bacteria. Therefore, we investigated the effect of resin coating materials on the attachment of Porphyromonas gingivalis (Pg). The polymerized auto cure resin plates were coated with two resin coating materials. To estimate the Pg attachment, each plate was immersed in brain heart infusion medium containing Pg. The quantity of bacteria attached on each plate was evaluated by crystal violet quantification. Morphological change of Pg was recorded using scanning electron microscopy. Both coating groups presented significantly lower Pg attachment compared to the control. The Pg shapes on the plates with resin coating materials were similar to the non-treated control plates. The resin coating materials clearly prevent Pg attachment on the polymerized auto cure resin plate.

    DOI: 10.4012/dmj.2011-164

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  • A problem-based learning tutorial on elder inpatients in a nursing home in Japan. 査読

    Matsuka Y, Nakajima R, Miki H, Kimura A, Kanyama M, Minakuchi H, Shinkawa S, Takiuchi H, Nawachi K, Maekawa K, Arakawa H, Fujisawa T, Sonoyama W, Mine A, Hara ES, Kikutani T, Kuboki T

    Journal of Dental Education   2012年

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  • Effect of low-shrinking composite on the bonding effectiveness of two adhesives in occlusal Class-I cavities.

    Atsushi Mine, Jan De Munck, Marcio V Cardoso, Kirsten L Van Landuyt, André Poitevin, Takuo Kuboki, Yasuhiro Yoshida, Kazuomi Suzuki, Bart Van Meerbeek

    Dental materials journal   31 ( 3 )   418 - 26   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of this study was to evaluate if a low-shrinking composite can improve the bonding effectiveness of adhesives in highly constrained conditions. A low-shrinking composite ('els-extra low shrinkage', Saremco) was bonded in standardized occlusal Class-I cavities using a three-step ('cmf', Saremco) and a two-step etch-and-rinse adhesive ('XP Bond', Dentsply). Both adhesives were also combined with a conventional composite ('Z100', 3M ESPE). Half of the restored cavities were exposed to 20,000 thermo-cycles. 3-way ANOVA revealed a significant effect for the factors 'adhesive' and 'composite' (both p<0.0001), but not for 'thermo-cycling' (p=0.994). Significantly higher bond strengths were recorded for the low-shrinking composite than for the control composite, using either of the adhesives. The low-shrinking composite in combination with the three-step etch-and-rinse adhesive performed best in the high C-factor Class-I cavity. The two-step etch-and-rinse adhesive suffered strongly from polymerization-shrinkage stress, which could be partially restored by using the low-shrinking composite.

    DOI: 10.4012/dmj.2011-261

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  • Intradermal injection of Botulinum toxin type A alleviates infraorbital nerve constriction-induced thermal hyperalgesia in an operant assay 査読

    Kumada A, Matsuka Y, Spigelman I, Maruhama K, Yamamoto Y, Neubert J.K, Nolan T.A, Watanabe K, Maekawa K, Kamioka H, Yamashiro T, Kuboki T, Oguma K

    Journal of Oral Rehabilitation   39 ( 1 )   63 - 72   2012年

  • Application of purified botulinum type a neurotoxin to treat experimental trigeminal neuropathy in rats and patients with urinary incontinence and prostatic hyperplasia. 査読 国際誌

    Yoshizo Matsuka, Teruhiko Yokoyama, Yumiko Yamamoto, Tomonori Suzuki, Ni Nengah Dwi Fatmawati, Atsushi Nishikawa, Tohru Ohyama, Toshihiro Watanabe, Takuo Kuboki, Atsushi Nagai, Keiji Oguma

    Journal of toxicology   2012   648384 - 648384   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Type A neurotoxin (NTX) of Clostridium botulinum was purified by a simple procedure using a lactose gel column. The toxicity of this purified toxin preparation was retained for at least 1 year at -30°C by supplementation with either 0.1% albumin or 0.05% albumin plus 1% trehalose. When purified NTX was used to treat 49 patients with urinary incontinence caused by either refractory idiopathic or neurogenic detrusor overactivity, 36 patients showed significant improvement in symptoms. These beneficial effects were also observed in cases of prostatic hyperplasia. The results obtained with NTX were similar to that of Botox. The effects of NTX on trigeminal neuralgia induced by infraorbital nerve constriction (IoNC) in rats were also studied. Trigeminal ganglion neurons from ipsilateral to IoNC exhibited significantly faster onset of FM4-64 release than sham-operated contralateral neurons. Intradermal injection of NTX in the area of IoNC alleviated IoNC-induced pain behavior and reduced the exaggerated FM4-64 release in trigeminal ganglion neurons.

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  • Occlusal dysesthesia: a qualitative systematic review of the epidemiology, aetiology and management 査読

    Hara, E. S., Matsuka, Y., Minakuchi, H., Clark, G. T., Kuboki, T.

    Journal of Oral Rehabilitation   39 ( 8 )   630 - 638   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Summary Occlusal dysesthesia refers to a persistent complaint of uncomfortable bite sensation with no obvious occlusal discrepancy. This systematic review aimed to draw a picture of such patients, to present an agreement of previously reported diagnostic criteria and to analyse the evidence level of the recommended management approaches. An electronic search for all relevant reports on occlusal dysesthesia was thoroughly performed based on previous nomenclatures (e.g. phantom bite, occlusal hyperawareness) in PubMed and The Cochrane Library in July, 2011. A total of 84 reports were matched, among which only 11 studies were included after a two-step (abstract and detailed full-text revision) screening process. Additionally, a thorough manual review of reference lists of the included reports enabled the inclusion of two additional studies. Data analysis revealed that 37 occlusal dysesthesia patients presented a mean age of 51・7 ± 10・6 years and were predominantly women (male/female: 1/5・1) with symptom duration of more than 6 years (average: 6・3 ± 7・5 years) and with concomitant psychological disturbances (e.g. mood disorders, somatoform disorders, personality disorders). Only four authors presented diagnostic criteria for occlusal dysesthesia, which served as the basis for an agreement in the diagnostic criteria. Treatment approaches included psychotherapy, cognitive/behaviour therapy, splint therapy and prescription of anti-depressants or anti-anxiety drugs. Classification of evidence level of management approaches, however, revealed that most of them were expert opinions with single- or multiple-case report(s). Future studies are necessary for a deeper understanding of the mechanisms behind the occlusal dysesthesia symptoms, and consequently, for improvements in evidence-based management approaches.

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  • Response shift in oral health-related quality of life measurement in patients with partial edentulism 査読

    Kimura, A., Arakawa, H., Noda, K., Yamazaki, S., Hara, E. S., Mino, T., Matsuka, Y., Mulligan, R., Kuboki, T.

    Journal of Oral Rehabilitation   39 ( 1 )   44 - 54   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purposes of this study were to determine whether a response shift was observable after partial denture treatment and to identify the predictors that influenced the response shift magnitude and direction. A total of 173 consecutive patients with no more than eight missing teeth who received implant-supported, fixed or removable partial dentures at Okayama University Dental Hospital were asked to complete a full-version Oral Health-Related Quality of Life (OHRQoL) questionnaire before (pre-test) and after treatment (post-test). Additionally, a short form (then-test) consisting of seven questions selected from the full version had its reliability verified and was utilised to retrospectively assess the pre-treatment OHRQoL status. The difference between the summary scores of the then-test and the pre-test determined the response shift magnitude and direction. The then-test mean score (22・9 ± 6・6) was significantly lower (worse OHRQoL) than that of the pre-test (26・4 ± 5・2). The response shift effect size was of moderate magnitude and negative direction (d = -0・78). A multiple regression analysis showed that age (younger patients) (P &lt; 0・01), number of replaced teeth (fewer) (P &lt; 0・01) and pre-test scores (lower) (P &lt; 0・01) were the significant predictors for response shift. In conclusion, a response shift phenomenon with negative and moderate effect size was observed after partial denture treatment. The significant predictor variables were young age, fewer numbers of replaced teeth and lower pre-test scores.

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  • The association between oral health-related quality of life and self-rated presenteeism 査読

    Takiuchi Hiroya, Kimura-Ono Aya, Arakawa Hikaru, Mino Takuya, Masaki Asuka, Hara Emilio Satoshi, Kanyama Manabu, Matsuka Yoshizo, Kuboki Takuo

    Quality of Life Research   21   71 - 72   2012年

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  • RELATIONSHIP BETWEEN SLEEP BRUXISM FREQUENCY AND UPTAKE ABILITY OF HUMAN PERIPHERAL SEROTONIN TRANSPORTER 査読

    Minakuchi H, Hara E. S, Sogawa C, Miki H, Fujisawa T, Maekawa K, Matsuka Y, Sogawa N, Kitayama S, Kuboki T

    Sleep   34   A201 - A202   2011年

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  • Peripheral administration of botulinum toxin type A neurotoxin reverses trigeminal neuropathic pain symptoms 査読

    Ai Kumada, Yoshizo Matsuka, Igor Spigelman, John K Neubert, Todd A Nolan, Yumiko Yamamoto, Keiko Sato, Kazuhiro Watanabe, Takuo Kuboki, Keiji Oguma

    http://f1000.com/posters/browse/summary/763   2011年

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    記述言語:英語   掲載種別:研究論文(大学,研究機関等紀要)  

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  • The relationship between temporomandibular joint pathosis and muscle tenderness in the orofacial and neck/shoulder region. 査読

    Eitoku Inoue, Kenji Maekawa, Hajime Minakuchi, Chiyomi Nagamatsu-Sakaguchi, Tsuyoshi Ono, Yoshizo Matsuka, GT Clark, Takuo Kuboki

    Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics   Vol.109 ( No.1 )   86 - 90   2010年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    These results indicated painful disk displacement to possibly be correlated with ipsilateral muscle tenderness.

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  • Novel Transcriptional Regulation of CCN2/CTGF by Nuclear Translocation of MMP3 査読

    Takanori Eguchi, Satoshi Kubota, Kazumi Kawata, Yoshiki Mukudai, Junji Uehara, Toshihiro Ohgawara, Soichiro Ibaragi, Akira Sasaki, Takuo Kuboki, Masaharu Takigawa

    CCN PROTEINS IN HEALTH AND DISEASE: AN OVERVIEW OF THE FIFTH INTERNATIONAL WORKSHOP ON THE CCN FAMILY OF GENES   255 - +   2010年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:SPRINGER-VERLAG BERLIN  

    CCN2/CTGF, previously known as Connective Tissue Growth Factor, is a crucial regulator of extra-cellular matrix (ECM), which promotes ECM synthesis and stabilization. As their family name clearly implies, matrix metalloproteases (MMPs) are also localized in the ECM, where they function as proteases, modulating cell signaling by cleaving proteins such as matrix proteins, growth factors and growth factor receptors. Strong expression of CCN2/CTGF in chondrocytic cells occurs through transcription enhancer dominant in chondrocytes (TRENDIC). Matrix metalloprotease-3 (MMP3) is a novel TRENDIC-binding transcription factor for CCN2/CTGF expression. First, MMP3 cDNA was cloned as a TRENDIC-binding factor by Southwestern screening. The interaction between MMP3 and TRENDIC was confirmed by a gel shift assay and chromatin immunoprecipitation. The CCN2/CTGF promoter was activated by transfected MMP3, whereas a TRENDIC mutant for the promoter lost the response. In addition, the knockdown of MMP3 suppressed CCN2/CTGF expression. Cytochemical and histochemical analyses demonstrated that MMP3 was detected in the nuclei of chondrocytic cells in culture and also in the nuclei of normal and osteoarthritic chondrocytes in vivo. The nuclear translocation of externally added recombinant MMP3 was observed in 30 min after the addition, and six putative nuclear localization signals were found in MMP3. These results indicated a novel trans-activation mechanism of CCN2/CTGF by the nuclear translocation of MMP3 through binding with TRENDIC in chondrocytes. Although MMPs historically had been recognized as a protease for extra-cellular proteins, this study indicated that it also stimulates ECM synthesis through CCN2/CTGF trans-activation. This novel regulatory role of the ECM may contribute to understanding the mechanism of not only the development, but also the pathogenesis of arthritis fibrosis and periodontitis.

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    その他リンク: http://orcid.org/0000-0002-9600-4430

  • 顎関節症の診療ガイドラインに関するプロジェクト研究—スプリント治療ガイドラインの確立—. 査読

    皆木省吾, 築山能大, 有馬太郎, 市川哲雄, 窪木拓男, 兒玉直紀, 佐久間重光, 新谷明喜, 高津匡樹, 竹内哲男, 津賀一弘, 坪井明人, 中島啓一朗, 中野雅徳, 成田紀之, 波多野泰夫, 藤澤政紀, 船登雅彦, 鱒見進一, 松香芳三

    日歯会誌   29   62 - 66   2010年

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  • Peripheral administration of botulinum toxin type A decreases exaggerated neurotransmitter release and reverses neuropathy-induced gene expression changes in trigeminal ganglia 査読

    Ai Kumada, Yoshizo Matsuka, Igor Spigelman, Yoshihito Ishihara, Yumiko Yamamoto, Takayuki Hikasa, Wataru Sonoyama, Hiroshi kamioka, Takashi Yamashiro, Takuo Kuboki, Keiji Oguma

    http://f1000.com/posters/browse/summary/87   2010年

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    記述言語:英語   掲載種別:研究論文(大学,研究機関等紀要)  

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  • 顎関節症のスプリント治療に関する実態調査 -大学病院顎関節症外来および一般開業歯科医院における診療時間の比較- 査読

    兒玉直紀, 築山能大, 有馬太郎, 市川哲雄, 窪木拓男, 佐久間重光, 新谷明喜, 高津匡樹, 津賀一弘, 坪井明人, 中野雅徳, 成田紀之, 波多野泰夫, 藤澤政紀, 船登雅彦, 鱒見進一, 松香芳三, 皆木省吾

    日本顎関節学会雑誌   21 ( 3 )   222 - 227   2009年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.11246/gakukansetsu.21.222

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  • Simvastatin induces the odontogenic differentiation of human dental pulp stem cells in vitro and in vivo. 査読

    Yosuke Okamoto, Wataru Sonoyama, Mitsuaki Ono, Kentaro Akiyama, Takuo Fujisawa, Masamitsu Oshima, Yohei Tsuchimoto, Yoshizo Matsuka, Tatsuji Yasuda, Songtao Shi, Takuo Kuboki

    Journal of Endodontics   Vol.35 ( No.3 )   367 - 372   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Statin, 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, is known to promote bone formation. However, it is not clear whether statin affects the differentiation of pulp cells. This study used a cell proliferation assay, cell cycle analysis, quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and in vivo transplantation to examine the effects of simvastatin on human dental pulp stem cells (DPSCs) in vitro and in vivo. Simvastatin at 1 mumol/L was able to significantly suppress the proliferation of DPSCs without inducing apoptosis. Quantitative RT-PCR revealed both osteocalcin and dentin sialophosphoprotein to be significantly up-regulated when DPSCs were cultured with simvastatin in comparison to bone morphogenetic protein-2 treatment. The in vivo transplantation data showed that simvastatin treatment promoted mineralized tissue formation. Taken together, these results suggest that statin might be an ideal active ingredient to accelerate the differentiation of DPSCs.

    DOI: 10.1016/j.joen.2008.11.024

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  • Corrigendum to "botulinum toxin type A (150 kDa) decreases exaggerated neurotransmitter release from trigeminal ganglion neurons and relieves neuropathy behaviors induced by infraorbital nerve constriction" [Neuroscience 159 (2009) 1422-1429] (DOI:10.1016 査読

    Kitamura Y, Matsuka Y, Spigelman I, Ishihara Y, Yamamoto Y, Sonoyama W, Kamioka H, Yamashiro T, Kuboki T, Oguma K

    Neuroscience   161 ( 3 )   2009年

  • Botulinum toxin blocks neurotransmitter release from somata of rat neuropathic trigeminal ganglion neurons 査読

    Kitamura Yoichi, Matsuka Yoshizo, Ishihara Yoshihito, Spigelman Igor, Yamamoto Yumiko, Sonoyama Wataru, Kamioka Horoshi, Yamashiro Takashi, Oguma Keiji, Kuboki Takuo

    TOXICON   51   6   2008年6月

  • 粘膜圧迫により疼痛の軽減がみられた三叉神経に生じた持続性ニューロパシー性疼痛の2例 査読

    松香 芳三, 熊田 愛, 前川 賢治, 水口 一, 窪木 拓男

    日本口腔顔面痛学会雑誌   Vol.1 ( No.1 )   55 - 59   2008年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.11264/jjop.1.55

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  • Promotion of hydroxyapatite-associated, stem cell-based bone regeneration by CCN2. 査読 国際誌

    Mitsuaki Ono, Satoshi Kubota, Takuo Fujisawa, Wataru Sonoyama, Harumi Kawaki, Kentaro Akiyama, Kengo Shimono, Masamitsu Oshima, Takashi Nishida, Yasuhiro Yoshida, Kazuomi Suzuki, Masaharu Takigawa, Takuo Kuboki

    Cell transplantation   17 ( 1-2 )   231 - 40   2008年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Multiple roles have been already recognized for CCN2 in cartilage development and regeneration. However, the effects of CCN2 on bone regeneration remain to be elucidated. In this study, the utility of CCN2 on bone regeneration was examined in vitro and in vivo in combination with hydroxyapatite (HAp) as a scaffold. Human bone marrow stromal cells (hBMSCs) were isolated from human iliac bone marrow aspirates of healthy donors and expanded, and the effects of CCN2 on their proliferation and migration were examined in vitro. The proliferation of hBMSCs on a plastic or HAp plate was significantly enhanced by CCN2. Moreover, the migration of hBMSCs also dramatically increased by CCN2. Interestingly, a C-terminal signal modular fragment of CCN2 (CT-module) also enhanced the cell proliferation and migration as efficiently as the full-length CCN2. Next, in order to estimate the effect of CCN2 on the migration and survival of hBMSCs and bone formation inside the HAp scaffold in vivo, two experiments were performed. First, the porous HAp carrier was cultured with hBMSCs for a week, and the cell-scaffold hybrid was transplanted with or without CCN2 subcutaneously into immunocompromised mice. CCN2 accelerated the hBMSC-like cell migration and survival inside the porous HAp within 4 weeks after transplantation. Second, the porous HAp carrier with or without CCN2 was directly implanted into bone defects within a rabbit mandible, and bone regeneration inside was evaluated. As a result, CCN2 efficiently induced the cell invasion and bone formation inside the porous HAp scaffold. These findings suggest that CCN2 and its CT-module fragment could be useful for regeneration and reconstruction of large-scale bone defects.

    DOI: 10.3727/000000008783907143

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  • Effect of surface pre-treatment on durability of resin-based cements bonded to titanium. 国際誌

    Youhei Tsuchimoto, Yasuhiro Yoshida, Maho Takeuchi, Atsushi Mine, Hirofumi Yatani, Yoh-ichi Tagawa, Bart Van Meerbeek, Kazuomi Suzuki, Takuo Kuboki

    Dental materials : official publication of the Academy of Dental Materials   22 ( 6 )   545 - 52   2006年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: Titanium has in recent years become a material of great opportunities in dentistry and orthopedics. In this study, we searched for a method to effectively pre-treat titanium as part of an adhesive luting and/or resin-veneering procedure. METHODS: Ti plates were treated with two different solutions of hydrochloric acid (0.1, 1N), 37wt% phosphoric acid or kept untreated (control). The tensile bond strength of the composite cement (Panavia Fluoro Cement, Kuraray) to the untreated and pre-treated Ti plates was determined without and after 20,000 thermo-cycles. XPS was used to chemically analyze the effect of the three Ti pre-treatments, as well as the interaction of 10-methacryloxydecyl dihydrogen phosphate (10-MDP; functional monomer of Panavia Fluoro Cement) with Ti treated with 1N HCl. RESULTS: Although no significant difference in immediate tensile bond strength was measured, thermo-cycling significantly decreased the bond strength of all experimental groups except for Ti treated with 1N HCl. No difference in surface roughness was found between untreated and treated Ti. XPS demonstrated that HCl effectively decontaminated Ti in a dose-dependent manner, while H(3)PO(4) was strongly adsorbed on the Ti surface. While the latter potentially inhibits adsorption of the phosphoric groups of the functional monomer 10-MDP, Ti pre-treated with 1N HCl improved the adsorption of 10-MDP as compared to untreated Ti. SIGNIFICANCE: It is concluded that 1N HCl effectively pre-treats Ti in order to make it more receptive to resin, while H(3)PO(4) should better not be used.

    DOI: 10.1016/j.dental.2005.08.002

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  • [Longitudinal follow-up of symptom severity of a patient with continuous neuropathic pain assessed by actual drug consumption record].

    Chiyomi Nagamatsu, Kumiko Nawachi, Atsushi Mine, Kenji Maekawa, Takuo Kuboki

    Nihon Hotetsu Shika Gakkai zasshi   49 ( 5 )   691 - 6   2005年10月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    PATIENT: The patient was a 56-year-old female, suffering from severe burning pain in the left maxillary alveolar area. The pain was initiated by periodontal curettage under no local anesthesia. No abnormal local findings in dental X-ray images and no abnormal central nervous system findings in brain magnetic resonance images were observed. Extraction of the tooth temporarily abated her pain, however the pain recurred gradually. Since the observed signs and symptoms of the patient agreed well with the diagnostic standard for continuous neuropathic pain (atypical alveolar pain) of Graff-Radford & Solberg (1992), medications (tricyclic antidepressant, non-steroidal anti-inflammatory drug (NSAID), anxiolytics, topical application of capsacin cream) and supportive psychological therapy have been administered continuously. To measure symptom severity over a long term, daily amounts of taken NSAID and anxiolytics were recorded continuously by the patient in a simple table diary for 7 years. As a result, the total amount of the drugs per month gradually decreased with the passage of time. DISCUSSION AND CONCLUSIONS: Clinical evidence regarding the prognosis of continuous neuropathic pain in the orofacial region is obviously insufficient, making it difficult to inform such patients of their prognosis of the disorder on the basis of clinical evidence. In this report, we have plotted the total amount of drugs taken per month by the patient as a good reference for the pain severity and the results clearly suggest that pain severity decreased with time. This kind of information may help both clinicians and patients for case-based explanation of the disorder.

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  • Adsorption of polyphosphoric acid to titanium surface and its effect on hBMSC attachment 査読

    K. Maekawa, A. Mine, T. Fujisawa, Y. Yoshida, K. Suzuki, T. Kuboki

    International Congress Series   1284   332 - 333   2005年9月

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    The aim of this study was to evaluate the effect of pre-treatment of titanium (Ti) surface with polyphosphoric acid on the initial attachment of human bone marrow derived mesenchymal stem cells (hBMSCs). The results of this study revealed that adsorption of polyphosphoric acid to Ti significantly enhanced initial attachment of hBMSCs. © 2005 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.ics.2005.06.091

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  • [Needs and current research directions of biological regenerative medicine in prosthodontic practice--to attain reliable and sophisticated dental implant therapy].

    Takuo Kuboki

    Nihon Hotetsu Shika Gakkai zasshi   49 ( 4 )   576 - 86   2005年8月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    Appropriate dental implants are known to improve the quality of life in totally and partially edentulous patients, as well as to prevent future tooth loss, and so prosthetic treatment modalities have drastically changed recently with reconstruction therapy. However, dental implant therapy did not win the confidence of the dental community in the early developmental stage, until osseointegration between titanium and surrounding bone tissue was discovered and the modality utilizing osseointegration became reliable and produced durable treatment outcomes for long-term function. On the other hand, the biological mechanism of osseointegration has not been clarified yet and the time required for osseointegration is still long, e.g. three to four months. As well, when the implant is applied in the upper posterior region, the acquisition of osseointegration and the long-term survival of the implant are still not clinically adequate. Therefore, to reduce the time required for osseointegration and to regenerate enough alveolar bone mass in the target implantation site, the oral implant modality must be made more useful and potent as one of the treatment options for partial and total edentulism. With this background, we have studied biological strategies for reducing the time required for osseointegration and for regenerating enough alveolar bone mass, e.g., investigation of the specific genes for osseointegration between titanium and bone, nano-level surface modification of the titanium, biodegradable apatite foam for alveolar bone regeneration, application of bone formation-related growth factors with biological scaffold, and autologous cell transplantation of bone marrow derived mesenchymal stem cells. In this article, we review the current status of regenerative medicine being applied in prosthodontics and discuss our future research direction.

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  • Regeneration of defects in articular cartilage in rat knee joints by CCN2 (connective tissue growth factor). 国際誌

    Takashi Nishida, Satoshi Kubota, Shunji Kojima, Takuo Kuboki, Kyouji Nakao, Toshihiro Kushibiki, Yasuhiko Tabata, Masaharu Takigawa

    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research   19 ( 8 )   1308 - 19   2004年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    UNLABELLED: CTGF/CCN2, a hypertrophic chondrocyte-specific gene product, possessed the ability to repair damaged articular cartilage in two animal models, which were experimental osteoarthritis and full-thickness defects of articular cartilage. These findings suggest that CTGF/CCN2 may be useful in regeneration of articular cartilage. INTRODUCTION: Connective tissue growth factor (CTGF)/CCN2 is a unique growth factor that stimulates the proliferation and differentiation, but not hypertrophy, of articular chondrocytes in vitro. The objective of this study was to investigate the therapeutic use of CTGF/CCN2. MATERIALS AND METHODS: The effects of recombinant CTGF/CCN2 (rCTGF/CCN2) on repair of damaged cartilage were evaluated by using both the monoiodoacetic acid (MIA)-induced experimental rat osteoarthritis (OA) model and full-thickness defects of rat articular cartilage in vivo. RESULTS: In the MIA-induced OA model, quantitative real-time RT-PCR assays showed a significant increase in the level of CTGF/CCN2 mRNA, and immunohistochemical analysis and in situ hybridization revealed that the clustered chondrocytes, in which clustering indicates an attempt to repair the damaged cartilage, produced CTGF/CCN2. Therefore, CTGF/CCN2 was suspected to play critical roles in cartilage repair. In fact, a single injection of rCTGF/CCN2 incorporated in gelatin hydrogel (rCTGF/CCN2-hydrogel) into the joint cavity of MIA-induced OA model rats repaired their articular cartilage to the extent that it became histologically similar to normal articular cartilage. Next, to examine the effect of rCTGF/CCN2 on the repair of articular cartilage, we created defects (2 mm in diameter) on the surface of articular cartilage in situ and implanted rCTGF/CCN2-hydrogel or PBS-hydrogel therein with collagen sponge. In the group implanted with rCTGF/CCN2-hydrogel collagen, new cartilage filled the defect 4 weeks postoperatively. In contrast, only soft tissue repair occurred when the PBS-hydrogel collagen was implanted. Consistent with these in vivo effects, rCTGF/CCN2 enhanced type II collagen and aggrecan mRNA expression in mouse bone marrow-derived stromal cells and induced chondrogenesis in vitro. CONCLUSION: These findings suggest the utility of CTGF/CCN2 in the regeneration of articular cartilage.

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  • [Cartilage and mechanical stress from the point of the view of development, growth, pathology and therapeutic aspects].

    Takuo Fujisawa, Masaharu Takigawa, Takuo Kuboki

    Clinical calcium   14 ( 7 )   29 - 35   2004年7月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    Articular cartilage is always subjected to various types, magnitudes and cycles of mechanical stress. While it has been recognized that these stresses regulate the chondrocyte growth and differentiation, the mechanism is still unclear. Here, we summarize the effect of mechanical stress on cartilage metabolism from the point of view of development, growth, pathology and therapeutic aspect.

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  • Connective tissue growth factor expressed in rat alveolar bone regeneration sites after tooth extraction. 国際誌

    Manabu Kanyama, Takuo Kuboki, Kentaro Akiyama, Kumiko Nawachi, Fabiora M Miyauchi, Hirofumi Yatani, Satoshi Kubota, Tohru Nakanishi, Masaharu Takigawa

    Archives of oral biology   48 ( 10 )   723 - 30   2003年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    OBJECTIVE: To understand bone regeneration process after tooth extraction could be a clue to develop a new strategy for alveolar bone reconstruction. Recently, accumulated evidences support that connective tissue growth factor (CTGF) is implicated in tissue repair of many tissues. In this study, we investigated the spatial and temporal expression of CTGF in the rat tooth extraction sockets. DESIGN: Five weeks old wild type male rats (weighing 120 g) were used for this experiment. Expression of CTGF was determined by immunohistochemistry and in situ hybridization in the rat upper molar tooth extraction sockets at 2, 4, 7, 10 and 14 days after tooth extraction. RESULTS: CTGF was expressed strongly in the endothelial cells migrating into the granulation tissue at the bottom of the sockets during 4 days after tooth extraction. During the reparative process, no apparent chondrocyte-like cell appeared in the sockets, while osteoblast-like cells proliferated in the sockets with low CTGF expression at 7, 10, 14 days after extraction. As expected, no staining was observed with the preimmune rabbit IgG and CTGF sense probe. CTGF may play an important role in angiogenesis and granulation tissue formation specifically at early healing stage after tooth extraction to initiate alveolar bone repair. CONCLUSION: CTGF was expressed at early healing stage of the rat tooth extraction wound.

    DOI: 10.1016/S0003-9969(03)00153-5

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  • A repetitive, steady mouth opening induced an osteoarthritis-like lesion in the rabbit temporomandibular joint

    T. Fujisawa, T. Kuboki, T. Kasai, W. Sonoyama, S. Kojima, J. Uehara, C. Komori, H. Yatani, T. Hattori, M. Takigawa

    Journal of Dental Research   82 ( 9 )   731 - 735   2003年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Intern. and American Associations for Dental Research  

    Although excessive mechanical stress is assumed to be one of the factors contributing to pathogenesis of temporomandibular joint (TMJ) osteoarthritis (OA), no pure mechanical-stress-induced OA model has been developed without surgical manipulation or puncture of the joint cavity. The purpose of this study was to establish a genuine mechanical-stress-induced OA model of the rabbit TMJ. In the experimental rabbits, repetitive, forced jaw-opening, 3 hrs/day for 5 days, was applied with the use of a general anesthesia protocol. By histological assessment of the TMJ articular tissues, partial eburnation of the articular cartilage, reactive marginal proliferation of the articular cartilage chondrocytes, and nested proliferation of chondrocytes in the subchondral bone area were observed at 7 days after the repetitive, forced-jaw-opening period. These results suggest that the repetitive, forced-jaw-opening protocol without surgical intervention can induce evident OA-like lesions in the rabbit TMJ, and this OA model may greatly contribute to the elucidation of the cartilage degradation mechanism in TMJ OA.

    DOI: 10.1177/154405910308200914

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  • 口腔アプライアンス療法が奏効した重度の閉塞性睡眠時無呼吸症症例 査読

    井上 栄徳, 窪木 拓男, 前川 賢治, 土井 郁世, 矢谷 博文, 宮脇 卓也, 佐藤 俊樹

    日本補綴歯科学会雑誌   46 ( 5 )   649 - 654   2002年12月

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    記述言語:日本語   出版者・発行元:(公社)日本補綴歯科学会  

    42歳男.強度のいびきと無呼吸を家族に指摘された.終夜睡眠ポリグラフィーでは無呼吸指数が33.4,無呼吸低呼吸指数が48.5で,重度の睡眠時無呼吸症と診断した.経鼻的持続陽圧呼吸療法は不快感から施行できず,口腔アプライアンス療法として,上顎と下顎に装着した全歯被覆型のアプライアンスを自作のハーベストタイプの金属製連結装置で連結し,下顎を相対的に前方位で保持させるツーピース型を使用した.治療開始時の下顎前方誘導量は,最大前方滑走量の50%程度の前突量,即ち切端咬合位からスタートし,1ヵ月に1度の頻度で連結装置に金属製のスペーサを追加して前方誘導量を増加させた.その結果,7ヵ月後には無呼吸指数4.5,無呼吸低呼吸指数9.7と著しく改善し,徐波睡眠も全睡眠時間の1.5%から17.4%に増加した.最終的な下顎前方誘導量は最大滑走量の70%であった

    DOI: 10.2186/jjps.46.649

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  • CTGF/Hcs24, a hypertrophic chondrocyte specific gene product, stimulates the proliferation and expression of the cartilage phenotype but not hypertrophy or calcification or articular cartilage in culture.

    Nishida T, Kubota S, Nakanishi T, Kuboki T, Yosimichi G, Kondo S, Takigawa M

    J Cell Physiol   192,   55 - 63   2002年

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  • Correlation of the near-infrared spectroscopy signals with signal intensity in T<inf>2</inf>-weighted magnetic resonance imaging of the human masseter muscle 査読

    Takuo Kuboki, Koji Suzuki, Kenji Maekawa, Mami Inoue-Minakuchi, Claver O. Acero, Yoshinobu Yanagi, Toru Wakasa, Kanji Kishi, Hirofumi Yatani, Glenn T. Clark

    Archives of Oral Biology   46   721 - 727   2001年7月

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    The purpose of this study was to compare and contrast blood volume changes transcutaneously measured using near-infrared (NIR) spectroscopy against water signal intensity changes taken from a transverse T2-weighted MR image of the masseter muscle in healthy human subjects before, during and after contraction. Eight healthy non-smoking males with no history of chronic muscle pain or vascular headaches participated (mean age: 23.9 ± 0.6 years). The MRI data were gathered using a turbo spin echo sequence (TR: 2300 ms; TE: 90 ms; FOV: 188 × 300 mm; scanning time: 30 s; slice thickness: 10 mm) and the slice level was set at the mid-point between the origin and insertion of the masseter. Intramuscular haemoglobin (Hb) levels and water content of the right masseter muscle were continuously monitored for 2 min before, 30 s during and 15 min after a maximum voluntary clenching (MVC) task. Both the near-infrared and MRI data were baseline-corrected and normalized and mean levels were established and plotted. Plots of the data showed that both near-infrared-based total Hb and T2-weighted MRI-based signal-intensity levels clearly decreased during contraction and a clear post-contraction rebound response was evident after the contraction. The near-infrared data were found to be highly correlated with MRI-based signal-intensity data (Pearson&#039;s r = 0.909, P &lt; 0.0001). In conclusion, these data provide powerful evidence that near-infrared data (total Hb), transcutaneously taken from the masseter muscle in humans, will reflect the intramuscular water signal intensity changes seen using a T2-weighted MRI imaging method. © 2001 Elsevier Science Ltd.

    DOI: 10.1016/S0003-9969(01)00030-9

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  • 一般歯科医院の受診患者における顎関節症の症状と徴候の変動 査読

    西村 真紀, 景山 正登, 松香 芳三, 畑中 乾志, 吉田 栄介, 水口 一, 窪木 拓男, 矢谷 博文

    日本顎頭蓋機能学会誌   Vol.14 ( No.1 )   23 - 31   2001年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • Biomechanical calculation of human TM joint loading with jaw opening 査読

    T Kuboki, Y Takenami, K Maekawa, M Shinoda, A Yamashita, GT Clark

    JOURNAL OF ORAL REHABILITATION   27 ( 11 )   940 - 951   2000年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    A three-dimensional, static mathematical calculation of the stomatognathic system was done to predict total temporomandibular joint (TMJ) loading at different levels of jaw opening. The model assumed that muscle forces acting on the mandible could be simulated by a combination of contractile components (CCs) and elastic components (ECs) and that static equilibrium existed within the body of the mandible. The model also imposed the constraint that any generated joint reaction force would act on the centre of the condyle. The results of the model demonstrated that under all conditions of opening and for all values of the elastic modulus selected, the forces between the TMJ condyle and the articular eminence were compressive in nature. The compressive force magnitude increased from 2 .7 to 27 .6 N incrementally as the jaw opened from 10 to 40 mm. Overall data in this study indicated that the TMJ tissues undergo low levels of compression at open positions up to 40mm. Finally, the condition of trismus (increased jaw closing activation with opening) was simulated, the Joint reaction force at 20 mm opening increased from 7 .7 to 64 .9 N with only a 20% activation of the dosers.

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  • Four-year longitudinal course of TMU symptoms in adult population and the estimation of risk factors in relation to the symptoms.(共著)

    Journal of Orofacial Pain   14 ( 3 )   224 - 232   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Haemodynamic responses in chronically painful, human trapezius muscle to cold pressor stimulation.(共著)

    Archires of Oral Bilogy   44 ( 10 )   805 - 812   1999年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S0003-9969(99)00082-5

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  • Direct adenovirus mediated gene delivery to the temporomandibular joint in guinea pigs. (共著)

    Archs Oral Biol   44 ( 9 )   701 - 709   1999年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S0003-9969(99)00069-2

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  • Effect of intravenous infusion of a beta-adrenergic blocking agent on the haemodynamic changes in human masseter muscle induced by cold pressor Stimulation. (共著)

    Archs Oral Biol   44 ( 6 )   475 - 483   1999年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S0003-9969(99)00028-X

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  • Effect of intravenous infusion of an alpha-adrenergic blocking agent on the haemodynamic changes in human masseter muscle induced by cold pressor stimulation. (共著)

    Archs Oral Biol   44 ( 4 )   319 - 327   1999年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S0003-9969(98)00127-7

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  • Haemodynamic response to cold pressor stimulation on chronically painful trapezius muscle 査読

    C.O.Acero, Jr, T.Kuboki, K.Maekawa, A.Yamashita, G.T.Clark

    Arch Oral Biol   1999年

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  • Connective tissue growth factor induces the proliferation, migration and tube formation of vascular endothelial cells in vitro and angiogenesis in vivo. (共著)

    J Biochemistry   126 ( 1 )   137 - 145   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/oxfordjournals.jbchem.a022414

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  • Cyclic mechanical stress induces extracellular matrix degradation in cultured chondrocytes via gene expression of matrix metalloproteinases and interleukin-1. (共著)

    J Biochemistry   125 ( 5 )   966 - 975   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/oxfordjournals.jbchem.a022376

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  • The effects of sustained incisal clenching on the temporomandibular joint space. (共著)

    Dent Maxillofac Radiol   28 ( 4 )   214 - 218   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/sj.dmfr.4600443

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  • Effect of occlusal appliances and clenching on the internally-deranged temporomandibular joint space.(共著)

    J Orofac Pain   13 ( 1 )   38 - 48   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Clinical predictability of temporomandibular joint disc displacement.(共著)

    J Dent Res   78 ( 2 )   650 - 660   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/00220345990780020401

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  • Cold pressor stimulus temparature and resting masseter muscle haemodynamics in normal human subjects. (共著)

    Archs Oral Biol   43 ( 11 )   849 - 859   1998年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S0003-9969(98)00072-7

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  • Macroscopic osseous changes in the temporomandibular joint related to dental attrition in Japanese macaque skull. 査読

    Yoshizo Matsuka, T Iijima, K Suzuki, T Kuboki, A Yamashita

    Journal of Oral Rehabilitation   Vol.25 ( No.9 )   687 - 693   1998年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of this study was to investigate the relationship between osseous changes in the temporomandibular joint (TMJ) and dental attrition in the Japanese macaque. One hundred and thirty Japanese macaque skulls (54 male and 76 female) from animals which had been bred in the same environment, were randomly sampled from a collection at the Primate Research Institute of Kyoto University. The age at death had been recorded in all cases. TMJ osseous changes were independently evaluated by three examiners, and were defined as an irregular surface or a perforated compact bone layer with a markedly irregular surface on either the temporal or condylar components. Age was a significant factor in predicting TMJ osseous changes (P &lt; 0.001). A strong relation was observed between age and dental attrition (P &lt; 0.001), while dental attrition was not a significant factor in predicting TMJ osseous changes (P = 0.334). The prevalence TMJ osseous changes in male animals was slightly higher than in females (P = 0.057). The results of this study suggest that osseous changes in the macaque TMJ are mainly related to age, not to dental attrition.

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  • The relationship between signs and symptoms of temporomandibular disorders and bilateral occlusal contact patterns during lateral excursions

    E. K. Watanabe, H. Yatani, T. Kuboki, Y. Matsuka, S. Terada, M. G. Orsini, A. Yamashita

    Journal of Oral Rehabilitation   25 ( 6 )   409 - 415   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Blackwell Publishing Ltd  

    The relationship between signs and symptoms of temporomandibular disorders (TMD) and bilateral occlusal contact patterns was investigated in 143 TMD patients (mean age: 34.0 ± 15.9 years
    38 male and 105 female). In addition to an interview regarding chief complaints and accompanying symptoms, various muscles and the temporomandibular joints were palpated bilaterally and occlusal analyses were made. Only 5 out of 108 paired variables were found to be significantly associated by using the chi-squared test. Medial pterygoid muscle pain on palpation showed significant associations with the occlusal contact pattern (P &lt
    0.005), especially working side contacts (interocclusal tooth contacts on the working side) (P &lt
    0.005), during contralateral excursions
    sternocleidomastoid muscle pain on palpation showed a significant association with balancing side contacts (interocclusal tooth contacts on the balancing side) during ipsilateral excursions P &lt
    0.05)
    shoulder stiffness and pain in the eye showed significant associations with balancing side contacts during contralateral excursions (P &lt
    0.05). The results show only a weak relationship between some TMD symptomatology and bilateral occlusal contact patterns during lateral excursions. The findings suggesting the specific laterality of a few TMD signs and symptoms associated with particular occlusal contacts may deserve closer case-control study. © 1998 Blackwell Science Ltd.

    DOI: 10.1046/j.1365-2842.1998.00262.x

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  • Diagnostic value of four criteria to interpret temporomandibular joint normal disc position on magnetic resonance images. (共著)

    Oral Surg Oral Med Oral Pathol Oral Radiol Endod   86 ( 4 )   489 - 497   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S1079-2104(98)90380-8

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  • Viscoelastic properties of the pig temporomandibular joint articular soft tissues of the condyle and disc 査読

    T Kuboki, M Shinoda, MG Orsini, A Yamashita

    JOURNAL OF DENTAL RESEARCH   76 ( 11 )   1760 - 1769   1997年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC DENTAL RESEARCH  

    It has been suggested that a sustained loading condition such as clenching could compress the temporomandibular joint (TMJ) articular soft tissues. However, there is still no clear understanding of how the TM joint articular tissues respond under compression. To answer this question, we performed in vitro indentation tests on fresh articular discs and cartilage-bone systems of the condyles of 10 Yorkshire pigs (aged 7 months) using a self-developed indentation tester. The indenter was 5 mm in diameter and was controlled by means of a computer-aided feedback mechanism. Bilateral condyles from the same mandible were uniformly prepared; one was used for measurements under sustained compression (SC) and the other for measurements under intermittent compression (IC). The displacements of the indenter induced by a SC of 10, 20, and 30 Newtons (N, units of force) for 10 min and by an IC, also of 10, 20, and 30 N, with one-second duration and two-second intervals for 10 min were measured by means of a displacement sensor with a resolution of 0.001 mm. From these data, the indentation curves of the articular discs and the cartilage-bone systems were calculated. Both the disc and the articular cartilage showed characteristic displacement vs. time curves-namely, an instantaneous deformation upon load application, followed by a time-dependent creep phase of asymptotically increasing deformation under constant load. However, the indentation curves of the two tissues were not identical: The deformation of the articular cartilage was dose-dependent, but that of the disc was not. Moreover, the articular cartilage deformed significantly less under IC than under SC. This difference was not found in the disc. It can be concluded that both the disc and the articular cartilage of the pig temporomandibular joint have viscoelastic properties against compression; however, the disc is stiffer than the articular cartilage.

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  • ニホンザル顎関節の形態計測学的研究 査読

    松香 芳三, 飯島 輝明, 鈴木 康司, 窪木 拓男, 矢谷 博文, 山下 敦, 上月 生也, 鈴木 秀典

    京都大学霊長類研究所年報   Vol.26   81 - 81   1997年

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    記述言語:日本語   掲載種別:研究論文(大学,研究機関等紀要)  

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  • Long-term Follow-up Study on Drop-out TMD Patients with Self-administered Questionnaires. (共著)

    J. Orafac. Pain   11 ( 3 )   258 - 269   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • The Influence of Observer Calibration in Temporomandibular Joint Magntic Resonance Imaging Diagnosis (共著)

    Oral Surg. Oral Med. Oral Pathol. Oral Raidol. Endod.   84 ( 1 )   82 - 87   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S1079-2104(97)90301-2

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  • The effect of occlusal appliances and clenching on the temporomandibular joint space

    Takuo Kuboki, Yoshiharu Azuma, Maria G. Orsini, Tetsuhiko Hirooka, Hirofumi Yatani, Atsushi Yamashita

    Journal of Orofacial Pain   11 ( 1 )   67 - 77   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    It has been suggested that stabilization appliances and mandibular anterior repositioning appliances ivork by decompressing the temporomandibular joint. To indirectly test this assumption, tomograms of right temporomandibular joints of seven subjects were taken during comfortable closure and maximum clenching in maximum intercuspation and on the two types of occlusal appliances. Outlines of the condyle and the temporal fossa were automatically determined by an edge detection protocol. Upon comfortable closure, the anterior joint space dimension was reduced with stabilization appliances and mandibular anterior repositioning appliances. Upon maximum clenching, the minimum joint space dimension on stabilization appliances was equivalent to that seen in maximum intercuspation, while that on mandibular anterior repositioning appliances was substantially less (P &lt
    .05). Findings do not indicate that these appliances induce an increase in joint space during clenching.

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  • Effect of Appliances and Clenching on the Temporomandibular Joint Space(共著)

    J. Orofac. Pain   11 ( 1 )   67 - 77   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Temporomandibular Disorders in the Adult Population of Okayama City, Japan(共著)

    J. Craniomandib. Pract.   14 ( 2 )   158 - 162   1996年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/08869634.1996.11745962

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  • Effect of Sustained Unilateral Molar Clenching on the Temporomendibular Joint Space. (共著)

    Oral Surg Oral Med Oral Pathol Oral Radio Endod   82 ( 6 )   616 - 624   1996年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S1079-2104(96)80435-5

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  • Effect of Sustained Unilateral Molar Clenching on the Temporomandibular Joint Space(共著)

    Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod.   82 ( 6 )   616 - 624   1996年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S1079-2104(96)80435-5

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  • A CASE-REPORT OF A PATIENT WITH UNILATERAL ANTERIOR DISK DISPLACEMENT (RECIPROCAL CLICKING) FINALIZED BY OCCLUSAL PORCELAIN BONDING

    H YATANI, T KUBOKI, Y AMANO, A YAMASHITA

    PATHOPHYSIOLOGY OF HEAD AND NECK MUSCULOSKELETAL DISORDERS   7   27 - 33   1990年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:KARGER  

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▼全件表示

書籍等出版物

  • 垂直的および水平的歯槽堤増大術 ソーセージテクニックと新たなコンビネーショングラフト

    クインテッセンス出版株式会社  2017年 

     詳細を見る

  • よくわかる口腔プラント学第3版.

    医歯薬出版株式会社  2017年 

     詳細を見る

  • 平成28年度岡山県医療介護総合確保基金事業「要介護高齢者の低栄養を防ぐための医師・歯科医師と管理栄養士による口腔栄養関連サービス推進事業」実績報告書.

    岡山大学病院  2017年 

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  • 口腔顔面痛の診断と治療ガイドブック第2版

    医歯薬出版株式会社  2016年 

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  • 平成27年度岡山県医療介護総合確保基金事業「要介護高齢者の低栄養を防ぐための医師・歯科医師と管理栄養士による口腔栄養関連サービス推進事業」実績報告書.

    岡山大学病院  2016年 

     詳細を見る

  • 平成27年度地域医療再生基金「要介護高齢者の低栄養を防ぐための県民公開講座」実績報告書.

    岡山大学病院  2016年 

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  • 咬合のサイエンスとアート

    クイッテンス出版株式会社  2016年 

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  • 骨・関節・軟骨治療のための新製品開発と臨床ニーズ. 治療薬,診断薬,診断装置,健康食品

    技術情報協会  2015年  ( ISBN:9784861045950

     詳細を見る

  • クラウンブリッジ補綴学第5版

    医歯薬出版  2014年  ( ISBN:9784263457832

     詳細を見る

  • 日本歯科評論

    株式会社ヒョーロン・パブリッシャーズ  2014年 

     詳細を見る

  • 日本歯科評論

    株式会社ヒョーロン・パブリッシャーズ  2014年 

     詳細を見る

  • 日本歯科評論別冊2014 65歳以上の患者へのインプラント治療・管理ガイド-要介護になっても対応できるために-

    株式会社ヒョーロン・パブリッシャーズ  2014年 

     詳細を見る

  • 日本歯科評論別冊2014 65歳以上の患者へのインプラント治療・管理ガイド-要介護になっても対応できるために-

    株式会社ヒョーロン・パブリッシャーズ  2014年 

     詳細を見る

  • 歯界展望

    医歯薬出版  2014年 

     詳細を見る

  • 口腔科学

    朝倉書店  2013年  ( ISBN:9784254350012

     詳細を見る

  • 新編 顎関節症

    永末書店  2013年  ( ISBN:9784816012617

     詳細を見る

  • 写真でマスターする顎関節症治療のためのスプリントのつくり方・つかい方

    ヒョーロンパブリッシャーズ  2011年 

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  • 顎関節症に対する関節鏡視下手術,Arthroscopy for temporomandibular disorders

    コクランレビューアブストラクト  2011年 

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  • 歯垢除去ならびに歯肉の健康における電動歯ブラシの相違Different powered toothbrushes for plaque control and gingival health

    コクランレビューアブストラクト  2011年 

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  • 顎関節症治療のための歯列矯正治療 Orthodontics for treating temporomandibular joint (TMJ) disorders,Cochrane Database of Systematic Reviews

    コクランレビューアブストラクト  2011年 

     詳細を見る

  • 口腔科学 歯の欠損と機能障害に対する治療1)クラウンブリッジによる歯牙欠損治療(6)メインテナンス

    朝倉書店  2010年 

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  • 別冊 YEAR BOOK 2010 2010年の歯科臨床19トレンド [2]エビデンスの成熟した活用を求めて,Part 4. 2010ホームデンティストの視点 TMDとエビデンス

    ザ・クインテッセンス出版  2010年 

     詳細を見る

  • チェアーサイド歯科英会話 ―分かる伝わるコミュニケーション!―

    吉備人出版  2010年 

     詳細を見る

  • 欠損歯を補うための介入:1回法と2回法インプラント治療,Interventions for replacing missing teeth: 1- versus 2-stage implant placement

    コクランレビューアブストラクト  2010年 

     詳細を見る

  • 口腔科学 5.2 歯の欠損と機能障害に対する治療1)クラウンブリッジによる歯牙欠損治療(5)可撤性ブリッジ

    朝倉書店  2010年 

     詳細を見る

  • コクランレビューアブストラクト

    コクランレビューアブストラクト  2009年 

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  • クラウンブリッジ補綴学

    医歯薬出版株式会社  2009年 

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  • 岡山TLO技術シーズ集 岡山県産業振興財団

    2008年 

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  • 補綴臨床別冊 審美歯科・インプラントワードブック 2008年春号

    医歯薬出版株式会社,東京  2008年 

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  • 補綴臨床別冊 審美歯科・インプラントワードブック 2008年春号

    医歯薬出版,東京  2008年 

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  • 補綴臨床別冊 審美歯科・インプラントワードブック 2008年春号

    東京  2008年 

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  • 補綴臨床別冊 審美歯科・インプラントワードブック 2008年春号

    東京  2008年 

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  • 補綴臨床別冊 審美歯科・インプラントワードブック 2008年春号

    東京  2008年 

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  • 補綴臨床別冊 審美歯科・インプラントワードブック 2008年春号

    東京  2008年 

     詳細を見る

  • 日本歯科医学会雑誌『平成19年度委託研究課題』報告書

    日本歯科医学会  2008年 

     詳細を見る

  • 平成17-20年度 科学研究費補助金(基盤研究B)実績報告書

    2008年 

     詳細を見る

  • 平成19-20年度 科学研究費補助金(基盤研究C)実績報告書

    2008年 

     詳細を見る

  • 平成18-19年度 科学研究費補助金(基盤研究C)研究成果報告書

    2008年 

     詳細を見る

  • コクランレビューアブストラクト

    2008年 

     詳細を見る

  • コクランレビューアブストラクト

    2008年 

     詳細を見る

  • コクランレビューアブストラクト

    2008年 

     詳細を見る

  • コクランレビューアブストラクト

    2008年 

     詳細を見る

  • コクランレビューアブストラクト

    2008年 

     詳細を見る

  • 平成17-18年度 科学研究費補助金(基盤研究C)実績報告書

    2006年 

     詳細を見る

  • Management of temporomandibular Disorders and Occlusion

    医歯薬出版  2006年 

     詳細を見る

  • Management of temporomandibular Disorders and Occlusion

    医歯薬出版  2006年 

     詳細を見る

  • 平成17-20年度 科学研究費補助金(基盤研究A)実績報告書

    2006年 

     詳細を見る

  • からだの科学増刊 高度先進医療

    日本評論社,東京  2005年 

     詳細を見る

  • よくわかる口腔インプラント学

    医歯薬出版,東京  2005年 

     詳細を見る

  • 歯界展望 特別号 健康な心と身体は口腔から-発 ヨコハマ 2004-

    医歯薬出版,東京  2005年 

     詳細を見る

  • 歯界展望 特別号 健康な心と身体は口腔から -発 ヨコハマ 2004-

    医歯薬出版,東京  2005年 

     詳細を見る

  • 平成14-16年度科学研究費補助金(基盤研究B)研究成果報告書

    2005年 

     詳細を見る

  • 平成15-16年度科学研究費補助金(基盤研究C)研究成果報告書

    2005年 

     詳細を見る

  • 平成15-16年度科学研究費補助金(基盤研究C)研究成果報告書

    2005年 

     詳細を見る

  • Interface Oral Health Science

    Elsevier Science,Amsterdam  2005年 

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  • 臨床における不安と疑問

    ヒョーロン・パブリッシャーズ,東京  2004年 

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  • う蝕治療のミニマルインターベンション

    クインテッセンス出版,東京  2004年 

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  • 平成14-15年度科学研究費補助金(基盤研究C)研究成果報告書

    2004年 

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  • 平成14-15年度科学研究費補助金(基盤研究C)研究成果報告書

    2004年 

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  • 目で見る咬合の基礎知識 月刊歯科技工別冊

    医歯薬出版,東京  2002年 

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  • 目で見る咬合の基礎知識 月刊歯科技工別冊

    医歯薬出版,東京  2002年 

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  • 目で見る咬合の基礎知識 月刊歯科技工別冊

    医歯薬出版,東京  2002年 

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  • 口腔顔面痛-基礎から臨床へ-

    クインテッセンス出版、東京  2001年 

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  • 口腔顔面痛-基礎から臨床へ-

    クインテッセンス出版、東京  2001年 

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  • 顎関節症入門

    医師薬出版  2001年 

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  • より良いエビデンスを求めて

    永末書店  2001年 

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  • 最新生理咬合学と顎関節症の治療

    クインテッセンス出版株式会社  1993年 

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  • The Modern Neuromuscular Occlusion and Management of Craniomandibular Disorders

    Quintessence Books Publishing Co.  1993年 

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  • The Modern Neuromuscular Occlusion and Management of Craniomandibular Disorders

    Quintessence Books Publishing Co.  1993年 

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  • 顎関節のバイオメカニクス-顎関節部負荷を科学する-

    Dental Diamond 増刊号顎関節症 こんな患者が来院したら  1991年 

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  • Preralence of craniomandibular dysfunction in adult populations of Okayama city, Japan(共著)

    Advanced Prosthodontics Worldwide  1991年 

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  • Temporomandibular joint remodeling following conservative treatment of patients with disk displacement(共著)

    Advanced Prosthodontics Worldwide  1991年 

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  • Biomechanics of the Masticatory System-

    Dental Diamond Supplmentum.  1991年 

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  • 平成18-20年度 科学研究費補助金(基盤研究B)実績報告書

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  • 平成18-19年度 科学研究費補助金(基盤研究C)実績報告書

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  • 平成18-19年度 科学研究費補助金(基盤研究C)実績報告書

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  • 平成18-19年度 科学研究費補助金(基盤研究C)実績報告書

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  • 平成17-20年度 科学研究費補助金(基盤研究B)実績報告書

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  • 平成18-19年度 科学研究費補助金(萌芽研究)実績報告書

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  • 平成18-19年度 科学研究費補助金(基盤研究C)実績報告書

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  • 平成18-19年度 科学研究費補助金(基盤研究C)実績報告書

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  • 平成18-19年度 科学研究費補助金(基盤研究C)実績報告書

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  • 平成17-20年度 科学研究費補助金(基盤研究B)実績報告書

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  • 平成18-19年度 科学研究費補助金(萌芽研究)実績報告書

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  • 平成17-19年度 科学研究費補助金(基盤研究C)実績報告書

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  • 平成18-21年度 科学研究費補助金(基盤研究B)実績報告書

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  • 平成17-18年度 科学研究費補助金(基盤研究C)実績報告書

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  • 平成17-20年度 科学研究費補助金(基盤研究A)実績報告書

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▼全件表示

MISC

  • Secondary Publication 補綴歯科治療の難易度を測定するプロトコルの妥当性の検討 (公社)日本補綴歯科学会による多施設臨床研究

    窪木 拓男, 市川 哲雄, 馬場 一美, 藤澤 政紀, 佐藤 博信, 會田 英紀, 小山 重人, 秀島 雅之, 佐藤 裕二, 和気 裕之, 大野 彩, 木村, 永尾 寛, 上田 順加, 平, 玉置 勝司, 貞森 紳丞, 津賀 一弘, 西 恭宏, 澤瀬 隆, 越野 寿, 鱒見 進一, 櫻井 薫, 石橋 寛二, 大山 喬史, 赤川 安正, 平井 敏博, 佐々木 啓一, 古谷野 潔, 矢谷 博文, 松村 英雄

    日本補綴歯科学会誌   11 ( 4 )   355 - 375   2019年10月

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    記述言語:日本語   出版者・発行元:(公社)日本補綴歯科学会  

    目的:日本補綴歯科学会は、補綴歯科治療を必要とする患者の複雑な多様性を評価し、それをもとに難易度分類を行うことを目的に「補綴歯科治療の難易度を測定する多軸診断プロトコル」を作成し、すでに臨床上十分な信頼性を示すことを確認した。本研究では多施設臨床追跡研究により本プロトコルの妥当性を検討した。方法:新たに作成した多軸診断プロトコルは、口腔内の状態、身体社会的状態、口腔関連QOL(OHIP-J54)、精神心理学的状態の4つの診断軸からなる。それぞれグレード0(易しい)から3(難しい)の4段階評価を行う。日本補綴歯科学会の認定研修施設である大学病院補綴科9施設で、歯質もしくは歯列欠損に対し補綴歯科治療が必要と判断された初診患者を連続サンプリングした。補綴歯科治療前に、本プロトコルを用いて難易度評価を行った。そして補綴歯科治療後に、治療に要した時間、医療費などの医療資源および口腔関連QOL評価を行った。本プロトコルの構成概念妥当性は、治療前の術者の主観による難易度評価と、本プロトコルの各軸の難易度の相関を求め検討した。予測妥当性は、総医療費、総治療時間、治療前後の口腔関連QOL変化量をサロゲートエンドポイントとして用い、本プロトコルの4つの診断軸の難易度を統合した総合難易度との相関を求めた。結果と考察:口腔内の状態(Axis I)、身体社会的状態(Axis II)、口腔関連QOL(Axis III)と治療前の術者の主観による難易度評価は、有意な正の相関を示した。一方、精神心理学的状態(Axis IV)と治療前の術者の主観による難易度評価には相関が認められなかった。術前に本プロトコルで評価した患者の総合難易度は、治療の難易度とよく関連すると考えられるサロゲートエンドポイント(総治療費、総治療時間、口腔関連QOL改善量)のいずれとも有意に相関した。口腔内状況別にこの関係を精査すると、術前の総合難易度評価は、全部歯列欠損症例や部分歯列欠損症例においては、これらのサロゲートエンドポイントの一部と有意に相関が認められたが、全部歯列欠損と部分歯列欠損の混合症例や歯の問題の症例においては、それらのサンプルサイズが小さいこともあり有意差が認められなかった。結論:補綴歯科治療の難易度を初めて多軸診断することに成功した本プロトコルの総合難易度評価は、全部歯列欠損症例や部分歯列欠損においては、臨床使用上十分な予測妥当性を示すことがわかった。(著者抄録)

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  • 【歯学部における多職種連携教育の現状と取組事例の紹介】調査報告 歯学部多職種連携教育に関する全国調査

    片岡 竜太, 浅沼 直樹, 窪木 拓男, 越野 寿, 澤瀬 隆, 角 忠輝, 筒井 睦

    日本歯科医学教育学会雑誌   35 ( 1 )   32 - 34   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本歯科医学教育学会  

    歯学部における保健・医療・福祉・介護などの分野と連携した多職種連携教育の実態を把握し、情報を共有して各大学の多職種連携教育の推進や改善・充実に役立てる目的で、全国29歯科大学、歯学部を対象にアンケート調査を実施した。全校で多職種連携教育が実施されており、学部教育のみが5校(17%)、学部教育と臨床研修歯科医教育の両方が23校(79%)であった。必修で実施している場合、学部教育では医療施設、介護施設の両施設での実施は16校(55%)、臨床研修では4校(14%)であった。臨床実習に参加している資格保持者は学部教育・臨床研修歯科医教育ともに、看護師や大学教員以外の医師・歯科医師、歯科衛生士、歯科技工士が多かった。学部教育・臨床研修歯科医教育ともに、要介護高齢者施設における歯科医師の職務遂行、誤嚥性肺炎などの感染症予防を目的とした口腔ケアの遂行、病院病棟における周術期口腔管理が目標として多くみられた。

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  • 多職種連携教育の導入・充実を目指して 全国29歯科大学、歯学部多職種連携教育アンケート集計結果

    片岡 竜太, 浅沼 直樹, 窪木 拓男, 越野 寿, 澤瀬 隆, 角 忠輝, 筒井 睦

    日本歯科医学教育学会総会・学術大会プログラム・抄録集   37回   76 - 76   2018年7月

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    記述言語:日本語   出版者・発行元:(一社)日本歯科医学教育学会  

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  • 口腔インプラント支持暫間上部構造の形態を最終上部構造へ反映させるデジタル新技法を応用した一例 暫間上部構造を作製した作業模型を利用する方法

    黒崎 陽子, 三野 卓哉, 和泉 幸治, 瀬島 淳一, 上田 明広, 大野 彩, 前川 賢治, 窪木 拓男

    日本口腔インプラント学会誌   31 ( 2 )   E81 - E82   2018年6月

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    記述言語:日本語   出版者・発行元:(公社)日本口腔インプラント学会  

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  • MCSF orchestrates branching morphogenesis in developing submandibular gland tissue. 国際誌

    Gulsan Ara Sathi, Mahmoud Farahat, Emilio Satoshi Hara, Hiroaki Taketa, Hitoshi Nagatsuka, Takuo Kuboki, Takuya Matsumoto

    Journal of cell science   130 ( 9 )   1559 - 1569   2017年5月

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    記述言語:英語   出版者・発行元:The Company of Biologists  

    The importance of macrophages in tissue development and regeneration has been strongly emphasized. However, the specific roles of macrophage colony-stimulating factor (MCSF), the key regulator of macrophage differentiation, in glandular tissue development have been unexplored. Here, we disclose new macrophage-independent roles of MCSF in tissue development. We initially found that MCSF is markedly upregulated at embryonic day (E)13.5, at a stage preceding the colonization of macrophages (at E15.5), in mouse submandibular gland (SMG) tissue. Surprisingly, MCSF-induced branching morphogenesis was based on a direct effect on epithelial cells, as well as indirectly, by modulating the expression of major growth factors of SMG growth, FGF7 and FGF10, via the phosphoinositide 3-kinase (PI3K) pathway. Additionally, given the importance of neurons in SMG organogenesis, we found that MCSF-induced SMG growth was associated with regulation of neurturin expression and neuronal network development during early SMG development in an in vitro organogenesis model as well as in vivo These results indicate that MCSF plays pleiotropic roles and is an important regulator of early SMG morphogenesis.

    DOI: 10.1242/jcs.196907

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  • Limited interaction of a self-adhesive flowable composite with dentin/enamel characterized by TEM. 国際誌

    Atsushi Mine, Jan De Munck, Annelies Van Ende, André Poitevin, Mariko Matsumoto, Yasuhiro Yoshida, Takuo Kuboki, Kirsten L Van Landuyt, Hirofumi Yatani, Bart Van Meerbeek

    Dental materials : official publication of the Academy of Dental Materials   33 ( 2 )   209 - 217   2017年2月

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    記述言語:英語   出版者・発行元:ELSEVIER SCI LTD  

    OBJECTIVES: A new category of composite which does not require any acid etching or bonding protocol prior to application has been introduced. The purpose of this study was to characterize the interfacial ultra-structure at enamel and dentin surfaces by means of transmission electron microscopy (TEM). METHODS: Non-carious human third molars were randomly divided into 6 groups (bur-cut dentin, SiC-ground dentin, fractured dentin, bur-cut enamel, SiC-ground enamel, and un-cut enamel). After preparation of the respective surfaces, the self-adhesive flowable composite (Vertise Flow, Kerr) was applied. Non-demineralized/demineralized and non-stained/stained sections of 70-90nm were prepared following common TEM-specimen processing procedures. Additional specimens were immersed in an ammoniacal silver nitrate solution. RESULTS: The composite-dentin interface was free of voids and no de-bonding occurred during specimen preparation. For bur-cut and SiC-ground dentin, no surface demineralization was observed and the smear contained residual hydroxyapatite. On fractured dentin (i.e. without smear interference), a very thin interaction area of 100-200nm without apparent signs of surface demineralization was seen. When the composite was bonded to enamel, a distinct separation between the self-adhesive composite and enamel was present in all three groups. A tight interaction, yet without distinct dissolution of hydroxyapatite, could only be seen in some regions at bur-cut enamel where smear was absent or sparse. Silver nitrate infiltration was associated with the presence of smear. SIGNIFICANCE: The self-adhesive composite showed limited interaction with smear-covered substrates and aprismatic enamel, which explains its inferior diminished bonding capacity in comparison with current adhesives.

    DOI: 10.1016/j.dental.2016.11.010

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  • Multicentered epidemiological study of factors associated with total bacterial count in the saliva of older people requiring nursing care.

    Takashi Tohara, Takeshi Kikutani, Fumiyo Tamura, Mitsuyoshi Yoshida, Takuo Kuboki

    Geriatrics & gerontology international   17 ( 2 )   219 - 225   2017年2月

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    記述言語:英語   出版者・発行元:WILEY  

    AIM: To clarify whether the number of present teeth, independent of other well-known factors, was associated with the total bacterial count in the saliva of older people requiring care at nursing homes in a multicentered epidemiological survey. METHOD: The participants were 618 older people (mean age 86.8 ± 6.9 years; 122 men, 496 women) residing in 14 nursing homes across Japan. The dependent variable was the participant's salivary bacterial count, and the independent variables were basic demographic data, oral conditions and activity of daily living (measured by Barthel Index). Statistical analysis was first carried out by Student's t-test, Pearson's correlation coefficient analysis and Spearman's rank correlation coefficient analysis. Independent variables found to have a significant relationship to their salivary bacterial count by the univariate analyses were further examined by stepwise multivariate analysis. RESULTS: The independent variables shown by univariate analysis to have a significant positive relationship with higher salivary bacterial count were presence of food residue (P = 0.001), absence of mouth dryness (P = 0.001), need of oral care assistance (P = 0.001), inability to keep the mouth opened (P = 0.009), inability to gargle (P = 0.002), denture use (P = 0.004), higher number of present teeth (P = 0.006) and lower Barthel Index (P = 0.001). Subsequent multivariate analysis identified presence of food residue (P = 0.031), higher number of present teeth (P = 0.043) and lower Barthel Index (P = 0.001) as independent associated factors for higher salivary bacterial count. CONCLUSIONS: The present study found that presence of food residue, higher number of present teeth and decreased activity of daily living were significantly related to higher bacterial count in the saliva of older people requiring care. Geriatr Gerontol Int 2017; 17: 219-225.

    DOI: 10.1111/ggi.12695

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  • Reliability and validity of the patient disability-oriented diagnostic nomenclature system for prosthetic dentistry

    Yoshizo Matsuka, Yoshiyuki Hagiwara, Katsushi Tamaki, Hisahiro Takeuchi, Masanori Fujisawa, Takahiro Ono, Yoshihiro Tsukiyama, Kan Nagao, Kazuhiro Tsuga, Hideki Aita, Hisatomo Kondo, Kenji Fueki, Hiroaki Tsukasaki, Keisuke Nishigawa, Shogo Ozawa, Rika Kuwatsuru, Hajime Minakuchi, Toshimitsu Iinuma, Takashi Matsuura, Kanji Ishibashi, Shigehisa Fujii, Toshihiro Hirai, Keiichi Sasaki, Hirofumi Yatani, Yoshimasa Igarashi, Yuji Sato, Tetsuo Ichikawa, Tetsuo Yamamori, Takuo Kuboki, Kazuyoshi Baba, Kiyoshi Koyano, Hironobu Sato, Hideo Matsumura

    JOURNAL OF PROSTHODONTIC RESEARCH   61 ( 1 )   20 - 33   2017年1月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    Purpose: The Japan Prosthodontic Society (JPS) has proposed a new diagnostic nomenclature system (DNS), based on pathogenesis and etiology, to facilitate and improve prosthodontic treatment. This systemspecifies patient disability and the causative factor (i.e. "B (disability) caused by A (causative factor)''). The purpose of this study was to examine the reliability and validity of this DNS.
    Study selection: The JPS Clinical Guideline Committee assessed mock patient charts and formulated disease names using the new DNS. Fifty validators, comprising prosthodontic specialists and dental residents, made diagnoses using the same patient charts. Reliability was evaluated as the consistency of the disease names among the validators, and validity was evaluated using the concordance rate of the disease names with the reference disease names.
    Results: Krippendorff's a was 0.378 among all validators, 0.370 among prosthodontic specialists, and 0.401 among dental hospital residents. Krippendorff's alpha for 10 validators (3 specialists and 7 residents) with higher concordance rates was 0.524. Two validators (1 specialist and 1 resident) with the highest concordance rates had a Krippendorff's alpha of 0.648. Common disease names had higher concordance rates, while uncommon disease names showed lower concordance rates. These rates did not show correlation with clinical experience of the validator or time taken to devise the disease name.
    Conclusions: High reliability was not found among all validators; however, validators with higher concordance rates showed better reliability. Furthermore, common disease names had higher concordance rates. These findings indicate that the new DNS for prosthodontic dentistry exhibits clinically acceptable reliability and validity. (C) 2016 The Authors. Published by Elsevier Ltd on behalf of Japan Prosthodontic Society. This is an open access article under the CC BY-NC-ND license.

    DOI: 10.1016/j.jpor.2016.06.005

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  • Challenges for stem cell-based "regenerative prosthodontics".

    Kunimichi Niibe, Fumio Suehiro, Masamitsu Oshima, Masahiro Nishimura, Takuo Kuboki, Hiroshi Egusa

    Journal of prosthodontic research   61 ( 1 )   3 - 5   2017年1月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.jpor.2016.09.001

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  • 歯科再生医療の実現に向けた大型動物モデルにおける歯の再生.

    大島正充, 大野充昭, 辻孝, 窪木拓男

    BIOINDUSTRY.   34 ( 9 )   1 - 11   2017年

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  • A multi-centered epidemiological study evaluating the validity of the treatment difficulty indices developed by the Japan Prosthodontic Society.

    Kuboki T, Ichikawa T, Baba K, Fujisawa M, Sato H, Aita H, Koyama S, Hideshima M, Sato Y, Wake H, Kimura-Ono A, Nagao K, Kodaira-Ueda Y, Tamaki K, Sadamori S, Tsuga K, Nishi Y, Sawase T, Koshino H, Masumi SI, Sakurai K, Ishibashi K, Ohyama T, Akagawa Y, Hirai T, Sasaki K, Koyano K, Yatani H, Matsumura H

    Journal of Prosthodontic Research   56 ( 2 )   71 - 86   2017年

  • 調整済みの暫間上部構造の形態を口腔インプラントの最終上部構造へ反映させる新規デジタルワークフロー.

    和泉幸治, 三野卓哉, 黒﨑陽子, 大野 彩, 前川賢治, 窪木拓男

    日本口腔インプラント学会誌.   30 ( 4 )   294 - 300   2017年

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  • 地域包括ケアの多職種協働の中で,今まさに「歯科医師」が果たす役割は?

    日本歯科医学教育学会雑誌   33 ( 3 )   25 - 27   2017年

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  • いつまでもおいしく食べるために知っておきたいこと

    窪木拓男, 縄稚久美子, 水口真実, 前田あずさ

    要介護高齢者の低栄養を防ぐための医師・歯科医師と管理栄養士による口腔栄養関連サービス推進事業パンフレット   1 - 6   2017年

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  • 歯学教育における診療参加型臨床実習のための電子版連携ログブック(電子ログブック)の開発と今後の課題について

    川瀬明子, 宮脇卓也, 小河達之, 窪木拓男, 久保田聡, 浅海淳一

    岡山歯学会雑誌   36 ( 2 )   53 - 59   2017年

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  • 虫歯になっても大丈夫?イヌの歯の再生に成功!

    窪木拓男, 大島正充, 大野充昭

    子供の科学.   80 ( 6 )   8 - 8   2017年

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  • 咀嚼筋の筋・筋膜痛に関連して生じたと考えられる歯肉の搔痒感を訴えた患者の1症例

    日本口腔顔面痛学会雑誌   9 ( 1 )   105 - 111   2017年

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  • Regenerative Medicine Reasearch with Bite

    Ono M, Hara ES, Kuboki T

    Nature/Nature Index 2017 Japan   543 ( 7645 )   S32-S32   2017年

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  • 軟骨内骨化中に最初に形成されるミネラルの同定.

    Hara ES, 岡田正弘, 長岡紀幸, 窪木拓男, 松本卓也

    日本歯科理工学会誌   36 ( 2 )   119 - 119   2017年

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  • MSCs feeder layers induce SMG self-organization and branching morphogenesis. 国際誌

    Mahmoud Farahat, Gulsan Ara Sathi, Emilio Satoshi Hara, Hiroaki Taketa, Takuo Kuboki, Takuya Matsumoto

    PloS one   12 ( 4 )   e0176453 - 5435   2017年

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    記述言語:英語   出版者・発行元:Public Library of Science ({PLoS})  

    Dysfunction of salivary glands leads to several oral health problems, including dental caries, mastication and swallowing dysfunctions and multiple oral infections. Conventional treatments for such condition fell short of providing satisfying therapeutic results. Recent advances in organ regeneration therapy which utilize tissue stem cells to fabricate bioengineered 3D organ buds, have introduced a promising therapeutic tool for full functional organ regeneration. However, finding a sustainable and easily accessible cell source for such approaches is still challenging, especially in case of severely atrophied tissues such as irradiated salivary glands. In response to this, we hypothesized that bone marrow derived mesenchymal stem cells (MSCs) could be used as feeder cells to induce salivary epithelial tissues/cells branching. Indeed, in 2D cultures, MSCs supported branching of embryonic submandibular salivary gland (SMG) epithelium. Interestingly, this enhancing effect was dependent on the initial number of MSC feeder cells. In addition, MSCs supported the self-assembly of SMG epithelial progenitor cells into well-patterned and branched 3D salivary organoids. Therefore, these findings propose MSCs as a valuable candidate cell source for induced SMG epithelial branching, which can potentially be applied in future methods for SMG regeneration approaches.

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  • 高度な硬軟両組織マネージメントと補綴歯科治療のコラボレーション

    窪木拓男, 大島正充, 大野充昭

    日本補綴歯科学会雑誌   9 ( 2 )   132 - 135   2017年

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  • Effect of prosthetic restoration on masticatory function in patients with shortened dental arches: a multicentre study

    K. Fueki, Y. Igarashi, Y. Maeda, K. Baba, K. Koyano, K. Sasaki, Y. Akagawa, T. Kuboki, S. Kasugai, N. R. Garrett

    JOURNAL OF ORAL REHABILITATION   43 ( 7 )   534 - 542   2016年7月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    The aim of this multicentre study was to investigate the effect of prosthetic restoration for missing posterior teeth on mastication in patients with shortened dental arches (SDAs). Partially dentate patients who had an intact teeth in anterior region and missed distal molar(s) (2-12 missing occlusal units) classified as Kennedy Class I or Class II were recruited from seven university-based dental hospitals in Japan. Of the 125 subjects who underwent baseline (pre-treatment) and follow-up/post-treatment evaluation, 53 chose no replacement of missing teeth and 72 chose treatment with removable partial dentures (n = 53) or implant-supported fixed partial dentures (n = 19). Objective masticatory performance (MP) was evaluated using a gummy jelly test. Perception of chewing ability (CA) was rated using a food intake questionnaire. In the no-treatment group, mean MP and CA scores at baseline were similar to those at follow-up evaluation (P &gt; 005). In the treatment group, mean MP after treatment was significantly greater than the pre-treatment mean MP (P &lt; 005). However, the mean perceived CA in the treatment groups was similar at pre- and post-treatment (P &gt; 005). In a subgroup analysis of subjects in the treatment group, subjects with lower pre-treatment CA showed a significant CA increase after treatment (P = 0004), but those with higher pre-treatment CA showed a significant decrease in CA (P = 0001). These results suggest that prosthetic restoration for SDAs may benefit objective masticatory performance in patients needing replacement of missing posterior teeth, but the benefit in subjective chewing ability seems to be limited in subjects with perceived impairment in chewing ability before treatment.

    DOI: 10.1111/joor.12387

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  • 平成27年度にODAPUSプログラムで岡山大学に留学をした5か国6校の学生の意識調査

    藤田 麻里子, 柳 文修, 久富 美紀, 此内 浩信, 村上 純, 前田 あずさ, 窪木 拓男, 宮脇 卓也, 仲野 道代, 皆木 省吾, 浅海 淳一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集   35回   97 - 97   2016年7月

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    記述言語:日本語   出版者・発行元:日本歯科医学教育学会  

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  • Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects. 国際誌

    Hajime Minakuchi, Chiharu Sogawa, Haruna Miki, Emilio S Hara, Kenji Maekawa, Norio Sogawa, Shigeo Kitayama, Yoshizo Matsuka, Glenn Thomas Clark, Takuo Kuboki

    Sleep & breathing = Schlaf & Atmung   20 ( 1 )   271 - 6   2016年3月

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    記述言語:英語  

    PURPOSE: To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency. METHODS: Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity (V max), and an affinity constant (K m ) were assessed with a [(3)H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated. RESULTS: Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [(3)H] 5-HT uptake was observed (Spearman's correlation R (2) = 0.063, p = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, V max, and K m values (p = 0.08, 0.12, 0.71, and 0.68, respectively). CONCLUSIONS: Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.

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  • CCN4/WISP-1 positively regulates chondrogenesis by controlling TGF-β3 function 国際誌

    Yuya Yoshioka, Mitsuaki Ono, Azusa Maeda, Tina M. Kilts, Emilio Satoshi Hara, Hany Khattab, Junji Ueda, Eriko Aoyama, Toshitaka Oohashi, Masaharu Takigawa, Marian, F. Young, Takuo Kuboki

    Bone, Metabolic Bone Disease and Related Research   83   162 - 170   2016年2月

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  • Cellular Reprogramming Using Defined Factors and MicroRNAs. 国際誌

    Takanori Eguchi, Takuo Kuboki

    Stem cells international   2016   7530942 - 7530942   2016年

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    記述言語:英語   出版者・発行元:Hindawi Publishing Corporation  

    Development of human bodies, organs, and tissues contains numerous steps of cellular differentiation including an initial zygote, embryonic stem (ES) cells, three germ layers, and multiple expertized lineages of cells. Induced pluripotent stem (iPS) cells have been recently developed using defined reprogramming factors such as Nanog, Klf5, Oct3/4 (Pou5f1), Sox2, and Myc. This outstanding innovation is largely changing life science and medicine. Methods of direct reprogramming of cells into myocytes, neurons, chondrocytes, and osteoblasts have been further developed using modified combination of factors such as N-myc, L-myc, Sox9, and microRNAs in defined cell/tissue culture conditions. Mesenchymal stem cells (MSCs) and dental pulp stem cells (DPSCs) are also emerging multipotent stem cells with particular microRNA expression signatures. It was shown that miRNA-720 had a role in cellular reprogramming through targeting the pluripotency factor Nanog and induction of DNA methyltransferases (DNMTs). This review reports histories, topics, and idea of cellular reprogramming.

    DOI: 10.1155/2016/7530942

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  • 早期見学実習の一環として実施した外来患者エスコート実習の学生評価 実習直後と5年経過後の臨床実習終了時での振り返り評価との比較.

    吉田登志子, 山中玲子, 鈴木康司, 河野隆幸, 白井 肇, 塩津範子, 武田宏明, 兒玉直紀, 鳥井康弘, 窪木拓男

    日本歯科医学教育学会雑誌.   32 ( 2 )   84 - 92   2016年

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  • 審美歯科治療の構造化,標準化.

    窪木拓男, 鈴木秀典

    日本補綴歯科学会誌.   8 ( 2 )   159 - 160   2016年

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  • Antagonistic Effects of Insulin and TGF-β3 during Chondrogenic Differentiation of Human BMSCs under a Minimal Amount of Factors 国際誌

    Emilio Satoshi Hara, Mitsuaki Ono, Yuya Yoshioka, Junji Ueda, Yuri Hazehara, Hai Thanh Pham, Takuya Matsumoto, Takuo Kuboki

    Cells Tissues Organs, Acta Anatomica   201 ( 2 )   88 - 96   2016年

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  • 間葉系幹細胞の新しい機能-免疫調節細胞としての間葉系幹細胞-.

    秋山謙太郎, 古味佳子, 窪木拓男

    日本補綴歯科学会誌.   8 ( 4 )   346 - 353   2016年

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  • Fluocinolone Acetonide Is a Potent Synergistic Factor of TGF-β3-Associated Chondrogenesis of Bone Marrow-Derived Mesenchymal Stem Cells for Articular Surface Regeneration. 国際誌

    Emilio Satoshi Hara, Mitsuaki Ono, Hai Thanh Pham, Wataru Sonoyama, Satoshi Kubota, Masaharu Takigawa, Takuya Matsumoto, Marian F Young, Bjorn R Olsen, Takuo Kuboki

    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research   30 ( 9 )   1585 - 96   2015年9月

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    記述言語:英語  

    Articular cartilage repair remains a challenging problem. Based on a high-throughput screening and functional analysis, we found that fluocinolone acetonide (FA) in combination with transforming growth factor beta 3 (TGF-β3) strongly potentiated chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). In an in vivo cartilage defect model in knee joints of immunocompromised mice, transplantation of FA/TGF-β3-treated hBMSCs could completely repair the articular surface. Analysis of the intracellular pathways revealed that FA enhanced TGF-β3-induced phosphorylation of Smad2 and Smad3. Additionally, we performed a pathway array and found that FA activates the mTORC1/AKT pathway. Chemical inhibition of mTORC1 with rapamycin substantially suppressed FA effect, and inhibition of AKT completely repressed chondrogenesis of hBMSCs. Inhibition of glucocorticoid receptor with mifepristone also suppressed FA effect, suggesting that FA involves binding to the glucocorticoid receptor. Comparative analysis with other glucocorticoids (triamcinolone acetonide [TA] and dexamethasone [DEX]) revealed the unique ability of FA to repair articular cartilage surgical defects. Analysis of intracellular pathways showed that the mTORC1/AKT pathway and the glucocorticoid receptor was highly activated with FA and TA, but to a lesser extent with DEX. Collectively, these results show a unique ability of FA to enhance TGF-β3-associated chondrogenesis, and suggest that the FA/TGF-β3 combination may be used as major inducer of chondrogenesis in vitro. Additionally, FA/TGF-β3 could be potentially applied in a clinical setting to increase the efficiency of regenerative approaches based on chondrogenic differentiation of stem cells.

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  • Effect of prosthetic restoration on oral health-related quality of life in patients with shortened dental arches: a multicentre study

    K. Fueki, Y. Igarashi, Y. Maeda, K. Baba, K. Koyano, K. Sasaki, Y. Akagawa, T. Kuboki, S. Kasugai, N. R. Garrett

    JOURNAL OF ORAL REHABILITATION   42 ( 9 )   701 - 708   2015年9月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    The aim of this multicentre prospective study was to investigate the effect of prosthetic restoration for missing posterior teeth in patients with shortened dental arches (SDAs). SDA patients with 2-12 missing occlusal units (a pair of occluding premolars corresponds to one unit, and a pair of occluding molars corresponds to two units) were consecutively recruited from seven university-based dental hospitals in Japan. Patients chose no replacement of missing teeth or prosthetic treatment with removable partial dentures (RPDs) or implant-supported fixed partial dentures (IFPDs). Oral health-related quality of life (OHRQoL) was measured using the oral health impact profile (Japanese version - OHIP-J) at baseline and follow-up/post-treatment evaluation. Of the 169 subjects who completed baseline evaluation, 125 subjects (mean age; 63.0 years) received follow-up/post-treatment evaluation. No-treatment was chosen by 42% (53/125) of the subjects, and 58% (72/125) chose treatment with a RPD (n = 53) or an IFPD (n = 19). In the no-treatment (NT) group, the mean OHIP summary score at baseline was similar to that at follow-up evaluation (P = 0.69). In the treatment (TRT) group, the mean OHIP summary score decreased significantly after the RPD treatment (P = 0.002), and it tended to decrease, though not statistically significant (P = 0.18), after the IFPD treatment. The restoration of one occlusal unit was associated with a 1.2-point decrease in OHIP summary score (P = 0.034). These results suggest that the replacement of missing posterior teeth with RPDs or IFPDs improved OHRQoL. Prosthetic restoration for SDAs may benefit OHRQoL in patients needing replacement of missing posterior teeth.

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  • A longitudinal retrospective study of the analysis of the risk factors of implant failure by the application of generalized estimating equations.

    Kinji Noda, Hikaru Arakawa, Aya Kimura-Ono, Seiya Yamazaki, Emilio Satoshi Hara, Wataru Sonoyama, Kenji Maekawa, Kazuo Okura, Ayumi Shintani, Yoshizo Matsuka, Takuo Kuboki

    Journal of prosthodontic research   59 ( 3 )   178 - 84   2015年7月

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    記述言語:英語  

    PURPOSE: Many studies have identified risk factors for dental implant failure, although few have investigated the correlation among implant fixtures within single patients. A better analytical method may include repeated measures analysis including generalized estimating equations (GEE). This retrospective cohort study aimed to (1) identify the risk factors for failure of dental implantation and (2) evaluate an analytical method using GEE analysis. METHODS: We analyzed data on early and late implant failures in 296 patients providing 721 rough surface dental implants (2.44 implants per patient). Potential predictors of implant failure included age, gender, smoking, location of implant, use of bone augmentation, number of remaining teeth, opposing tooth condition, fixture length, fixture diameter and type of suprastructure (fixed or removable partial denture). The likelihood of early and late implant failure was estimated by GEE. RESULTS: The early failure rate was 1.5% (11/721 implants, 7/296 patients) and the 10-year cumulative survival rate was 94.0% (7/710 implants, 5/293 patients). The GEE analysis revealed that a significant risk factor for early implant failure was smoking (p<0.01), whereas significant risk factors for late failure were maxillary implant (p=0.02), posterior implant (p<0.01), number of remaining teeth (≥20) (p<0.01), opposing unit being a removable partial denture or nothing (p=0.04) and having a removable type suprastructure (p<0.01). CONCLUSIONS: GEE analysis showed that smoking was a risk factor for early implant failure, and several risk factors were identified for late implant failure.

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  • Fluocinolone Acetonide Is a Potent Synergistic Factor of TGF-β3-Associated Chondrogenesis of Bone Marrow-Derived Mesenchymal Stem Cells for Articular Surface Regeneration

    Hara ES, Ono M, Pham HT, Sonoyama W, Kubota S, Takigawa M, Matsumoto T, Young MF, Olsen BR, Kuboki T

    Jounal of Bone and Mineral Research   30 ( 9 )   1585 - 1596   2015年

  • A longitudinal retrospective study of the analysis of the risk factors of implant failure by the application of generalized estimating equations

    Noda, Kinji, Arakawa, Hikaru, Kimura-Ono, Aya, Yamazaki, Seiya, Hara, Emilio Satoshi, Sonoyama, Wataru, Maekawa, Kenji, Okura, Kazuo, Shintani, Ayumi, Matsuka, Yoshizo, Kuboki, Takuo

    Journal of Prosthodontic Research   59 ( 3 )   178 - 184   2015年

  • 歯の喪失ならびに口腔機能低下が栄養状態に及ぼす影響-アセスメント法の開発-

    菊谷 武, 吉田光由, 菅 武雄, 木村年秀, 田村文誉, 窪木拓男

    日本歯科医学会誌   34   59 - 63   2015年

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  • 患者のステージに応じた摂食・咀嚼・嚥下評価法とその対応方法に関する研究

    水口真実, 前川賢治, 菊谷 武, 田村文誉, 吉田光由, 大野友久, 玉田泰嗣, 近藤尚知, 木本克彦, 大川周治, 佐藤裕二, 鈴木浩司, 川良美佐雄, 窪木拓男

    日本歯科医学会誌   34   94 - 98   2015年

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  • 審美領域における口腔インプラント治療の予後の安定性.

    窪木拓男

    Quintessence DENTAL Implantology   22 ( 5 )   3 - 4   2015年

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  • コンピュータ支援による顎運動解析「貼付一体型睡眠時ブラキシズム検出・解析装置」

    水口 一, 窪木拓男

    日本歯科理工学会誌別誌歯科材料・器械 DE:The Journal of Dental Engineering.   34 ( 3 )   199 - 202   2015年

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  • The BMP2 antagonist inhibitor L51P enhances the osteogenic potential of BMP2 by simultaneous and delayed synergism

    Hany Mohamed Khattab, Mitsuaki Ono, Wataru Sonoyama, Yasutaka Oida, Shigehiko Shinkawa, Yuya Yoshioka, Kenji Maekawa, Yasuhiko Tabata, Kazushige Sugama, Walter Sebald, Talmo Kuboki

    BONE   69   165 - 173   2014年12月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    Bone morphogenetic protein 2 (BMP2) is a potent osteoinductive cytokine that plays crucial roles in bone repair. However, large amounts of BMP2 are required to induce sufficient bone formation in humans possibly due to a feedback response of BMP antagonists. The engineered BMP2 variant L51P is deficient in BMP receptor type I activation but maintains affinity for BMP antagonists and can allow for the inactivation of BMP antagonists, and eventually enhance BMP2 action. As hypothesized, simultaneous addition of L51P enhanced the BMP2-induced osteogenesis. To test the ability of L51P to competitively inactivate BMP antagonists, cell binding affinity of BMP2 ligands was investigated in the presence or absence of L51P. Because the BMP antagonists were highly expressed 3 days after exogenous BMP2 stimulation, we collected supernatants from 3-day stimulated cell cultures and used as condition culture media (CM). The results showed a significant decrease in the cell binding of BMP2 ligands when cells were incubated with exogenous BMP2 and CM, whereas L51P addition competitively rescued the suppression of BMP2-to-cell binding induced by CM incubation. In a delayed experimental model, L51P was applied 3 days after exogenous BMP2 stimulation and we could observe a striking enhancement of the BMP2-induced SMAD-1/5/8 phosphorylation and luciferase activity of the Id1 promoter compared to the simultaneous addition of the two factors. These findings provide a deeper insight into the cellular and molecular mechanisms involved in the effect of L51P in suppressing the BMP antagonists and enhancing BMP activity. Additionally, these results demonstrate that L51P is a promising down regulator of BMP-induced negative feedback, which could have a significant impact in future applications of BMP2 in research and clinical settings. (C) 2014 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bone.2014.09.011

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  • Functional tooth restoration by next-generation bio-hybrid implant as a bio-hybrid artificial organ replacement therapy. 国際誌

    Masamitsu Oshima, Kaoru Inoue, Kei Nakajima, Tetsuhiko Tachikawa, Hiromichi Yamazaki, Tomohide Isobe, Ayaka Sugawara, Miho Ogawa, Chie Tanaka, Masahiro Saito, Shohei Kasugai, Teruko Takano-Yamamoto, Takashi Inoue, Katsunari Tezuka, Takuo Kuboki, Akira Yamaguchi, Takashi Tsuji

    Scientific reports   4   6044 - 6044   2014年8月

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    記述言語:英語  

    Bio-hybrid artificial organs are an attractive concept to restore organ function through precise biological cooperation with surrounding tissues in vivo. However, in bio-hybrid artificial organs, an artificial organ with fibrous connective tissues, including muscles, tendons and ligaments, has not been developed. Here, we have enveloped with embryonic dental follicle tissue around a HA-coated dental implant, and transplanted into the lower first molar region of a murine tooth-loss model. We successfully developed a novel fibrous connected tooth implant using a HA-coated dental implant and dental follicle stem cells as a bio-hybrid organ. This bio-hybrid implant restored physiological functions, including bone remodelling, regeneration of severe bone-defect and responsiveness to noxious stimuli, through regeneration with periodontal tissues, such as periodontal ligament and cementum. Thus, this study represents the potential for a next-generation bio-hybrid implant for tooth loss as a future bio-hybrid artificial organ replacement therapy.

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  • 新規X線造影剤による変形性膝関節症ラット関節軟骨の高解像度マイクロCT造影

    大橋 俊孝, 加来田 博貴, 芳谷 学, 大月 孝志, 大野 充昭, 山田 翔也, 前原 亜美, 廣畑 聡, 西田 圭一郎, 窪木 拓男, 二宮 善文

    日本結合組織学会学術大会・マトリックス研究会大会合同学術集会プログラム・抄録集   46回・61回   106 - 106   2014年6月

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    記述言語:日本語   出版者・発行元:日本結合組織学会・マトリックス研究会  

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  • The regenerative effects of CCN2 independent modules on chondrocytes in vitro and osteoarthritis models in vivo. 国際誌

    Tarek Abd El Kader, Satoshi Kubota, Takashi Nishida, Takako Hattori, Eriko Aoyama, Danilo Janune, Emilio S Hara, Mitsuaki Ono, Yasuhiko Tabata, Takuo Kuboki, Masaharu Takigawa

    Bone   59 ( 59C )   180 - 8   2014年2月

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    記述言語:英語  

    The role of CCN family proteins has been proven to be of extreme importance in the process of cartilage development and endochondral ossification. The second member, CCN2, consists of 4 conserved modules that interact with a number of cofactors to display multiple functions. Although the potentially therapeutic effect of intact CCN2 on cartilage regeneration has been indicated by a number of studies, the regenerative effect of independent modules comprising CCN2 has never been evaluated before. This study aims to discover a more robust and effective CCN2 derivative to induce regeneration through assessing the effect of CCN2 independent modules on regeneration in vitro and in vivo, in comparison to the full length CCN2. In vitro evaluation using human chondrocytic cells showed a remarkable enhancing effect of several single modules on the gene expression of cartilaginous extracellular matrix components; whereas combinations of 2 or 3 modules rather diminished such effects. Interestingly, combination of all 4 modules redeemed the effect of intact CCN2 in vitro. Suspecting the re-assembly of the 4 modules, interaction among the modules was examined by surface plasmon resonance analysis. However, the results did not support the possible formation of a tetramodular complex. Next, the thrombospondin 1 type 1 repeat module (TSP1), which was found most promising in the experiments in vitro, and the combination of 4 modules were forwarded further to in vivo confirmation using 2 rat osteoarthritis (OA) models. As a result, TSP1 displayed more prominent regenerative effects than intact CCN2 on damaged cartilage. Unexpectedly, the combination of 4 modules showed limited effects in vivo. These results indicate the utility of TSP1 in the regenerative therapeutics of OA. Possible molecular mechanism that enables conditional reconstruction of CCN2 by 4 modules is discussed as well.

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  • The pathophysiology of heterotopic ossification: Current treatment considerations in dentistry

    Kengo Shimono, Kenta Uchibe, Takuo Kuboki, Masahiro Iwamoto

    Japanese Dental Science Review   50 ( 1 )   1 - 8   2014年2月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等  

    Heterotopic ossification (HO) consists of the formation of ectopic cartilage followed by endochondral bone, and is triggered by major surgeries, large wounds, and other conditions. Daily functions of HO patients can be hampered by the loss of normal posture, pain, inflammation, reduced mobility, formation of pressure ulcers, deep venous thrombosis, and other complications. Research so far revealed the molecular and cellular pathways leading HO formation, and proposed several possible mechanisms behind such pathways. Nonsteroidal anti-inflammatory drug (NSAID) regimens and localized low-dose irradiation are currently available as prophylaxis of HO formation. However, they are not always effective and do not target skeletogenic processes directly. New therapeutic modalities targeting pathological process of HO formation, such as bone morphogenetic proteins (BMP) inhibitors like Noggin, BMP type 1 receptor inhibitor, and nuclear retinoic acid receptor-gamma (RARγ) agonists are currently under investigation. In this review, we will summarize our current understanding of the pathology and molecular and cellular mechanisms of HO, especially endochondral heterotopic ossification, and then discuss its current and future therapies. We will also discuss the potential application of heterotopic ossification in the dental field. © 2013 Japanese Association for Dental Science.

    DOI: 10.1016/j.jdsr.2013.07.003

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  • Mesenchymal Stem/Progenitor Cell Isolation from Tooth Extraction Sockets

    Nakajima, R., Ono, M., Hara, E. S., Oida, Y., Shinkawa, S., Pham, H. T., Akiyama, K., Sonoyama, W., Maekawa, K., Kuboki, T.

    Journal of Dental Research   93 ( 11 )   1133 - 1140   2014年

  • 顎関節症はなぜ発症するのか

    前川賢治, 窪木拓男

    日本歯科評論   2014年

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  • Efficient bone formation in swine socket-lift model using E. coli-derived rhBMP-2 adsorbed in β-TCP.

    Ono M, Sonoyama W, Yamamoto K, Oida Y, Akiyama K, Shinkawa S, Nakajima R, Pham HT, Hara ES, Kuboki T

    Cells Tissues Organs   199 ( 4 )   249 - 255   2014年

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  • Occlusion and Weight Change in a Patient After Esophagectomy: Success Derived from Restoration of Occlusal Support

    Reiko Yamanaka, Yoshihiko Soga, Mami Minakuchi, Kumiko Nawachi, Takayuki Maruyama, Takuo Kuboki, Manabu Morita

    INTERNATIONAL JOURNAL OF PROSTHODONTICS   26 ( 6 )   574 - 576   2013年11月

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    記述言語:英語   出版者・発行元:QUINTESSENCE PUBLISHING CO INC  

    Occlusal support may be an important factor affecting nutritional support after major surgery. This report presents a patient who gained body weight after receiving a new prosthesis. The patient was an 82-year-old man with thoracic esophageal carcinoma. He did not have occlusal support because of multiple caries lesions. His body weight slowly increased after surgery, but almost stopped in the period of 54 to 68 days after surgery. After treatment with dentures (day 72 postsurgery), body weight gain was observed again, although his medical treatment had not changed. An appropriate prosthesis could contribute to perioperative nutrition support and may lead to earlier recovery after surgery.

    DOI: 10.11607/ijp.3622

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  • Retrospective investigation of the remaining teeth status of patients with implant-supported fixed partial dentures in unilateral free-end edentulism.

    Seiya Yamazaki, Hikaru Arakawa, Kenji Maekawa, Emilio Satoshi Hara, Kinji Noda, Hajime Minakuchi, Wataru Sonoyama, Yoshizo Matsuka, Takuo Kuboki

    Journal of prosthodontic research   57 ( 4 )   262 - 7   2013年10月

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    記述言語:英語  

    PURPOSE: This study aimed to compare the various complication-free rates and survival rates of remaining teeth among implant-supported fixed dentures (IFDs), removable partial dentures (RPDs) and no-restoration (NR) patients with unilateral free-end edentulism. MATERIAL AND METHODS: The study subjects were selected among those who received prosthodontic treatment at Okayama University Dental Hospital for their unilateral free-end edentulism (2 or 3 missing teeth). Thirty-three patients were included in the IFD group, 41 matched patients in the RPD group, and 10 patients who received RPDs but refused their use were regarded as NR group. The remaining dentition was classified into five subcategories in relation to the missing portion: adjacent teeth to the missing portion (AD), contralateral posterior dentition in the same jaw (CS) and in the opposite jaw (CO), ipsilateral opposing posterior dentition (IO), and anterior dentition (AN). Complications were defined as tooth extraction, periodontal lesions, periapical lesions or loss of retention of the prosthesis and were assessed by one examiner based on the hospital chart records. RESULTS: The cumulative complication-free rates in the remaining teeth were significantly different among each of the three groups (p<0.01), with a significantly lower incidence rate in the IFD group. Regarding the cumulative survival rate of the remaining teeth, there was a significant difference only between IFD and NR group (p=0.01), especially in the CO region (p=0.04). CONCLUSIONS: Stable posterior occlusal support obtained with IFD treatment for unilateral free-end edentulism may reduce the incidence of complications in the remaining teeth, by decreasing the adverse mechanical stress.

    DOI: 10.1016/j.jpor.2013.08.001

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  • 咬合違和感患者の治療予後に関する要因の多施設調査

    澁谷智明, 玉置勝司, 和気裕之, 島田淳, 葉山莉香, 古谷野潔, 鱒見進一, 窪木拓男, 皆木省吾, 貞森紳丞, 矢谷博文, 藤澤政紀, 杉崎正志, 成田紀之, 玉井和樹, 原節宏, 馬場一美, 尾口仁志, 金村清孝, 山口泰彦, 西川洋二, 塚原宏泰, 松香芳三

    日本顎関節学会雑誌   25 ( Suppl. )   103 - 103   2013年7月

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    記述言語:日本語   出版者・発行元:(一社)日本顎関節学会  

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  • Retrospective comparative ten-year study of cumulative survival rates of remaining teeth in large edentulism treated with implant-supported fixed partial dentures or removable partial dentures.

    Seiya Yamazaki, Hikaru Arakawa, Kenji Maekawa, Emilio Satoshi Hara, Kinji Noda, Hajime Minakuchi, Wataru Sonoyama, Yoshizo Matsuka, Takuo Kuboki

    Journal of prosthodontic research   57 ( 3 )   156 - 61   2013年7月

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    記述言語:英語  

    PURPOSE: This study aimed to compare the survival rates of remaining teeth between implant-supported fixed dentures (IFDs) and removable partial dentures (RPDs) in patients with large edentulous cases. The second goal was to assess the risk factors for remaining tooth loss. MATERIALS AND METHODS: The study subjects were selected among those who received prosthodontic treatment at Okayama University Dental Hospital for their edentulous space exceeding at least four continuous missing teeth. Twenty-one patients were included in the IFD group and 82 patients were included in the RPD group. Survival rates of remaining teeth were calculated in three subcategories: (1) whole remaining teeth, (2) adjacent teeth to intended edentulous space, and (3) opposing teeth to intended edentulous space. RESULTS: The ten-year cumulative survival rate of the whole remaining teeth was significantly higher in the IFD group (40.0%) than in the RPD group (24.4%). On the other hand, there was no significant difference between two groups in the survival rate of teeth adjacent or opposing to intended edentulous space. A Cox proportional hazard analysis revealed that RPD restoration and gender (male) were the significant risk factors for remaining tooth loss (whole remaining teeth). CONCLUSIONS: These results suggest that IFD treatment can reduce the incidence of remaining tooth loss in large edentulous cases.

    DOI: 10.1016/j.jpor.2013.03.003

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  • Consensus statement from JPS Global Workshop Kyoto 2012.

    Kiyoshi Koyano, Yoshinobu Maeda, Christian S Stohler, Regina Mericske-Stern, James D Hudson, Soon-Ho Yim, Hyeong-Seob Kim, Udey Vir Gandhi, Hironobu Sato, Takuo Kuboki, Hirofumi Yatani

    Journal of prosthodontic research   57 ( 3 )   153 - 5   2013年7月

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    記述言語:英語   掲載種別:会議報告等   出版者・発行元:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.jpor.2013.05.001

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  • 咬合異常感(違和感)の発症因子に関する多施設実態調査

    澁谷 智明, 玉置 勝司, 和気 裕之, 島田 淳, 古谷野 潔, 鱒見 進一, 窪木 拓男, 皆木 省吾, 貞森 紳丞, 矢谷 博文, 藤澤 政紀, 杉崎 正志, 成田 紀之, 原 節宏, 馬場 一美, 尾口 仁志, 金村 清孝, 山口 泰彦, 西川 洋二, 塚原 宏泰

    日本補綴歯科学会誌   5 ( 2 )   E169 - E169   2013年4月

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    記述言語:日本語   出版者・発行元:(公社)日本補綴歯科学会  

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  • L51P, a BMP-2 Antagonist Inhibitor, Enhances the Osteoiduction of BMP-2 by a Negative Feedback Regulation

    Hany Khattab, Mitsu Ono, Wataru Sonoyama, Yasutaka Oida, Shigehiko Shinkawa, Kenji Maekawa, Kazushige Sugama, Walter Sebald, Takuo Kuboki

    JOURNAL OF BONE AND MINERAL RESEARCH   28   2013年2月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:WILEY-BLACKWELL  

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  • OHIP日本語版を用いた補綴治療患者の口腔関連QOL評価における最小重要差の検討

    大野 彩, 木村, 窪木 拓男, 市川 哲雄, 秀島 雅之, 佐藤 裕二, 玉置 勝司, 赤川 安正, 永尾 寛, 櫻井 薫, 馬場 一美, 皆木 省吾, 鱒見 進一, 佐藤 博信, 澤瀬 隆, 西 恭宏, 和気 裕之, 大山 喬史, 平井 敏博, 佐々木 啓一

    日本補綴歯科学会誌   5 ( 1 )   E113 - E113   2013年1月

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    記述言語:日本語   出版者・発行元:(公社)日本補綴歯科学会  

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  • OstemiR: a novel panel of microRNA biomarkers in osteoblastic and osteocytic differentiation from mesencymal stem cells. 国際誌

    Takanori Eguchi, Ken Watanabe, Emilio Satoshi Hara, Mitsuaki Ono, Takuo Kuboki, Stuart K Calderwood

    PloS one   8 ( 3 )   e58796   2013年

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    記述言語:英語  

    MicroRNAs (miRNAs) are small RNA molecules of 21-25 nucleotides that regulate cell behavior through inhibition of translation from mRNA to protein, promotion of mRNA degradation and control of gene transcription. In this study, we investigated the miRNA expression signatures of cell cultures undergoing osteoblastic and osteocytic differentiation from mesenchymal stem cells (MSC) using mouse MSC line KUSA-A1 and human MSCs. Ninety types of miRNA were quantified during osteoblastic/osteocytic differentiation in KUSA-A1 cells utilizing miRNA PCR arrays. Coincidently with mRNA induction of the osteoblastic and osteocytic markers, the expression levels of several dozen miRNAs including miR-30 family, let-7 family, miR-21, miR-16, miR-155, miR-322 and Snord85 were changed during the differentiation process. These miRNAs were predicted to recognize osteogenic differentiation-, stemness-, epinegetics-, and cell cycle-related mRNAs, and were thus designated OstemiR. Among those OstemiR, the miR-30 family was classified into miR-30b/c and miR-30a/d/e groups on the basis of expression patterns during osteogenesis as well as mature miRNA structures. In silico prediction and subsequent qRT-PCR in stable miR-30d transfectants clarified that context-dependent targeting of miR-30d on known regulators of bone formation including osteopontin/spp1, lifr, ccn2/ctgf, ccn1/cyr61, runx2, sox9 as well as novel key factors including lin28a, hnrnpa3, hspa5/grp78, eed and pcgf5. In addition, knockdown of human OstemiR miR-541 increased Osteopontin/SPP1 expression and calcification in hMSC osteoblastic differentiation, indicating that miR-541 is a negative regulator of osteoblastic differentiation. These observations indicate stage-specific roles of OstemiR especially miR-541 and the miR-30 family on novel targets in osteogenesis.

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  • 実物大臓器立体モデルを活用した手術支援の実際と歯科技工士の関わり

    竹内哲男, 有地秀裕, 松本 洋, 中原龍一, 水川展吉, 吉岡徳枝, 木股敬裕, 尾崎敏文, 飯田征二, 佐々木朗, 三野卓哉, 前川賢治, 森田 学, 槇野博史, 窪木拓男

    QDT別冊 CAD/CAM YEAR BOOK 2013   別冊   22 - 31   2013年

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  • 矯正歯科治療患者の口腔関連Quality of Lifeの評価

    古森紘基, 川邊紀章, 片岡伴記, 白賀のり子, 大野 彩, 窪木拓男, 山城 隆

    岡山歯学会誌   31 ( 2 )   96 - 97   2013年

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  • miRNA-720 controls stem cell phenotype, proliferation and differentiation of human dental pulp cells. 国際誌

    Emilio Satoshi Hara, Mitsuaki Ono, Takanori Eguchi, Satoshi Kubota, Hai Thanh Pham, Wataru Sonoyama, Shoji Tajima, Masaharu Takigawa, Stuart K Calderwood, Takuo Kuboki

    PloS one   8 ( 12 )   e83545   2013年

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    記述言語:英語  

    Dental pulp cells (DPCs) are known to be enriched in stem/progenitor cells but not well characterized yet. Small non-coding microRNAs (miRNAs) have been identified to control protein translation, mRNA stability and transcription, and have been reported to play important roles in stem cell biology, related to cell reprogramming, maintenance of stemness and regulation of cell differentiation. In order to characterize dental pulp stem/progenitor cells and its mechanism of differentiation, we herein sorted stem-cell-enriched side population (SP) cells from human DPCs and periodontal ligament cells (PDLCs), and performed a locked nucleic acid (LNA)-based miRNA array. As a result, miR-720 was highly expressed in the differentiated main population (MP) cells compared to that in SP cells. In silico analysis and a reporter assay showed that miR-720 targets the stem cell marker NANOG, indicating that miR-720 could promote differentiation of dental pulp stem/progenitor cells by repressing NANOG. Indeed, gain-and loss-of-function analyses showed that miR-720 controls NANOG transcript and protein levels. Moreover, transfection of miR-720 significantly decreased the number of cells positive for the early stem cell marker SSEA-4. Concomitantly, mRNA levels of DNA methyltransferases (DNMTs), which are known to play crucial factors during stem cell differentiation, were also increased by miR-720 through unknown mechanism. Finally, miR-720 decreased DPC proliferation as determined by immunocytochemical analysis against ki-67, and promoted odontogenic differentiation as demonstrated by alizarin red staining, as well as alkaline phosphatase and osteopontin mRNA levels. Our findings identify miR-720 as a novel miRNA regulating the differentiation of DPCs.

    DOI: 10.1371/journal.pone.0083545

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  • 長期的服薬状況評価により,プレガバリンが著効を示したと判断された神経障害性歯痛の一症例

    前川賢治, 古味佳子, 窪木拓男

    日本口腔顔面痛学会雑誌   6 ( 1 )   13 - 17   2013年

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  • 神経障害性疼痛へのボツリヌス毒素の効果

    松香芳三, 丸濵功太郎, 三宅愛, 山本由弥子, 前川賢治, 古味佳子, 園山亘, 小熊惠二, 窪木拓男

    歯界展望   2013年

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  • Identification of risk factors for fracture of veneering materials and screw loosening of implant-supported fixed partial dentures in partially edentulous cases

    Noda, K., Arakawa, H., Maekawa, K., Hara, E. S., Yamazaki, S., Kimura-Ono, A., Sonoyama, W., Minakuchi, H., Matsuka, Y., Kuboki, T.

    Journal of Oral Rehabilitation   40 ( 3 )   214 - 220   2013年

  • A novel vibratory stimulation-based occlusal splint for alleviation of TMD painful symptoms: a pilot study

    Hara, E. S., Witzel, A. L., De Luca, C. E. P., Ballester, R. Y., Kuboki, T., Bolzan, M. C.

    Journal of Oral Rehabilitation   40 ( 3 )   179 - 184   2013年

  • A Retrospective Comparative 8-Year Study of Cumulative Complications in Teeth Adjacent to Both Natural and Implant-Supported Fixed Partial Dentures

    Yamazaki, Seiya, Arakawa, Hikaru, Maekawa, Kenji, Noda, Kinji, Hara, Emilio Satoshi, Minakuchi, Hajime, Sonoyama, Wataru, Matsuka, Yoshizo, Kuboki, Takuo

    International Journal of Prosthodontics   26 ( 3 )   260 - 264   2013年

  • Corrigendum to "A multi-centered epidemiological study evaluating the reliability of the treatment difficulty indices developed by the Japan Prosthodontic Society"

    Takuo Kuboki, Tetsuo Ichikawa, Kazuyoshi Baba, Masayuki Hideshima, Yuji Sato, Hiroyuki Wake, Kan Nagao, Yorika Kodaira-Ueda, Aya Kimura-Ono, Katsushi Tamaki, Kazuhiro Tsuga, Kaoru Sakurai, Hironobu Sato, Kanji Ishibashi, Hirofumi Yatani, Takashi Ohyama, Yasumasa Akagawa, Toshihiro Hirai, Keiichi Sasaki, Kiyoshi Koyano

    Journal of Prosthodontic Research   56   297   2012年10月

  • 咬合異常感(違和感)の発症因子に関する多施設による実態調査の結果報告

    玉置勝司, 古谷野潔, 鱒見進一, 窪木拓男, 皆木省吾, 貞森紳丞, 矢谷博文, 藤澤政紀, 杉崎正志, 成田紀之, 原節宏, 馬場一美, 尾口仁志, 金村清孝, 山口泰彦, 西川洋二, 塚原宏泰

    日本歯科医師会雑誌   65 ( 5 )   636 - 636   2012年8月

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  • Occlusal dysesthesia: a qualitative systematic review of the epidemiology, aetiology and management

    E. S. Hara, Y. Matsuka, H. Minakuchi, G. T. Clark, T. Kuboki

    JOURNAL OF ORAL REHABILITATION   39 ( 8 )   630 - 638   2012年8月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:WILEY-BLACKWELL  

    Occlusal dysesthesia refers to a persistent complaint of uncomfortable bite sensation with no obvious occlusal discrepancy. This systematic review aimed to draw a picture of such patients, to present an agreement of previously reported diagnostic criteria and to analyse the evidence level of the recommended management approaches. An electronic search for all relevant reports on occlusal dysesthesia was thoroughly performed based on previous nomenclatures (e.g. phantom bite, occlusal hyperawareness) in PubMed and The Cochrane Library in July, 2011. A total of 84 reports were matched, among which only 11 studies were included after a two-step (abstract and detailed full-text revision) screening process. Additionally, a thorough manual review of reference lists of the included reports enabled the inclusion of two additional studies. Data analysis revealed that 37 occlusal dysesthesia patients presented a mean age of 51.7 +/- 10.6 years and were predominantly women (male/female: 1/5.1) with symptom duration of more than 6 years (average: 6.3 +/- 7.5 years) and with concomitant psychological disturbances (e.g. mood disorders, somatoform disorders, personality disorders). Only four authors presented diagnostic criteria for occlusal dysesthesia, which served as the basis for an agreement in the diagnostic criteria. Treatment approaches included psychotherapy, cognitive/behaviour therapy, splint therapy and prescription of anti-depressants or anti-anxiety drugs. Classification of evidence level of management approaches, however, revealed that most of them were expert opinions with single- or multiple-case report(s). Future studies are necessary for a deeper understanding of the mechanisms behind the occlusal dysesthesia symptoms, and consequently, for improvements in evidence-based management approaches.

    DOI: 10.1111/j.1365-2842.2012.02300.x

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  • 補綴歯科治療の難易度を測定するプロトコルの信頼性の検討 : (社)日本補綴歯科学会による多施設臨床研究

    窪木 拓男, 市川 哲雄, 馬場 一美, 秀島 雅之, 佐藤 裕二, 和気 裕之, 永尾 寛, 上田 小平, 大野 木村, 玉置 勝司, 津賀 一弘, 櫻井 薫, 佐藤 博信, 石橋 寛二, 矢谷 博文, 大山 喬史, 赤川 安正, 平井 敏博, 佐々木 啓一, 古谷野 潔

    日本補綴歯科学会誌 = Annals of Japan Prosthodontic Society   56 ( 2 )   71 - 86   2012年4月

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    記述言語:英語   出版者・発行元:Japan Prosthodontic Society  

    目的:(社)日本補綴歯科学会は,補綴治療を行う患者の複雑な多様性を評価し,それをもとに難易度分類を行うことを目的に「補綴治療の難易度を測定するプロトコル」を新たに作成した.本研究では本プロトコルの内容を紹介するとともに,多施設臨床研究により本プロトコルの信頼性を検討した.方法:新たに作成した多軸診断プロトコルは,口腔内の形態学的状態,身体社会的状態,口腔関連QOL(OHIP-J54),精神心理学的状態の4つの診断軸からなる.それぞれグレード0(易しい)から3(難しい)の4段階評価を行う.日本補綴歯科学会の認定研修施設である大学病院補綴科5施設で,歯質もしくは歯列欠損に対し補綴治療が必要と判断された初診患者を連続サンプリングした.本プロトコルの妥当性を大規模なコホート研究により検討する前に信頼性の検討をテスト・リテスト法により行った.具体的には,同じ評価を約2週間の間隔をおいて2度行い,その結果の一致度を重み付けカッパ(Kw)値を算出することにより評価した.結果と考察:4つの診断軸のうち,口腔内の形態学的状態のテスト・リテスト一致度は,Landis and Koch1)の基準に照らすと「十分な一致」となった(全部歯列欠損群/部分歯列欠損群/歯質欠損群の平均Kw値:0.82/0.73/0.78).さらに,身体社会的状態(0.88),OHIP-J54総得点(0.74),精神心理学的状態(0.61)でも「十分な一致」もしくは「優秀な一致」となった.結論:補綴歯科治療の難易度を初めて多軸診断することに成功した本プロトコルは,臨床使用上十分な信頼性を示した.

    DOI: 10.1016/j.jpor.2012.02.007

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  • Real time assessment of surface interactions with a titanium passivation layer by surface plasmon resonance. 国際誌

    Isao Hirata, Yasuhiro Yoshida, Noriyuki Nagaoka, Kyou Hiasa, Yasuhiko Abe, Kenji Maekawa, Takuo Kuboki, Yasumasa Akagawa, Kazuomi Suzuki, Bart Van Meerbeek, Phillip B Messersmith, Masayuki Okazaki

    Acta biomaterialia   8 ( 3 )   1260 - 6   2012年3月

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    Due to the high corrosion resistance and strength to density ratio titanium is widely used in industry, and also in a gamut of medical applications. Here we report for the first time on our development of a titanium passivation layer sensor that makes use of surface plasmon resonance (SPR). The deposited titanium metal layer on the sensor was passivated in air, similarly to titanium medical devices. Our "Ti-SPR sensor" enables analysis of biomolecule interactions with the passivated surface of titanium in real time. As a proof of concept, corrosion of a titanium passivation layer exposed to acid was monitored in real time. The Ti-SPR sensor can also accurately measure the time-dependence of protein adsorption onto the titanium passivation layer at sub-nanogram per square millimeter accuracy. Besides such SPR analyses, SPR imaging (SPRI) enables real time assessment of chemical surface processes that occur simultaneously at "multiple independent spots" on the Ti-SPR sensor, such as acid corrosion or adhesion of cells. Our Ti-SPR sensor will therefore be very useful to study titanium corrosion phenomena and biomolecular titanium-surface interactions with application in a broad range of industrial and biomedical fields.

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  • Test-retest reliability of MRI-based disk position diagnosis of the temporomandibular joint. 国際誌

    Chiyomi Nagamatsu-Sakaguchi, Kenji Maekawa, Tsuyoshi Ono, Yoshinobu Yanagi, Hajime Minakuchi, Shouichi Miyawaki, Junichi Asaumi, Teruko Takano-Yamamoto, Glenn T Clark, Takuo Kuboki

    Clinical oral investigations   16 ( 1 )   101 - 8   2012年2月

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    記述言語:英語  

    This study evaluated the test-retest reliability for determining the temporomandibular joint (TMJ) disk position, diagnosed using magnetic resonance imaging (MRI). These assessments were done as a base-line measurement for a prospective cohort study, which examines the risk factors for precipitation and progression of temporomandibular disorders. Fifteen subjects (mean age, 24.2 ± 0.94 years; male/female = 8/7) were recruited from the students of Okayama University Dental School. Sagittal MR TMJ images were taken with a 1.5-T MR scanner (Magneton Vision, Siemens) in close and maximal open positions twice at about 1-week (6-11 days) interval. The images were displayed using 200% magnification on a computer screen with a commercially available image software package (OSIRIS, UIN/HCUG). Three calibrated examiners diagnosed the disk positions using the standardized criteria. The disk position of each joint was classified as normal, anterior disk displacement with or without reduction, and others. The first and second disk position diagnoses were compared, and the test-retest reliability level was calculated using the kappa index. The second disk position diagnosis was consistent with the first in 27 out of 30 joints. The calculated kappa value representing the test-retest reliability level between the first and second disk position diagnosis was 0.812. These results indicated that the test-retest reliability of MRI-based diagnosis of TMJ disk positions at about 1-week interval was substantially high, even though they were not completely consistent.

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  • プロビジョナルレストレーションを口腔機能に調和した補綴物に置き換えるための一手法

    樋口鎮央, 山地康之, 三野卓哉, 窪木拓男, 和田主実, 和田弘毅

    日本歯科産業学会誌   26 ( 2 )   37 - 44   2012年

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  • 文献と臨床の橋わたし 接着試験のデータを使いこなす 接着強さ(MPa)の真実と必要接着強さ

    峯 篤史, 窪木 拓男, 鈴木 一臣

    日本歯科評論   71 ( 10 )   164 - 166   2011年10月

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    記述言語:日本語   出版者・発行元:(株)ヒョーロン・パブリッシャーズ  

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  • TEM interfacial characterization of an experimental self-adhesive filling material bonded to enamel/dentin. 国際誌

    Masao Hanabusa, Atsushi Mine, Takuo Kuboki, Yasuko Momoi, Kirsten L Van Landuyt, Bart Van Meerbeek, Jan De Munck

    Dental materials : official publication of the Academy of Dental Materials   27 ( 8 )   818 - 24   2011年8月

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    記述言語:英語   出版者・発行元:ELSEVIER SCI LTD  

    UNLABELLED: A great challenge regarding the ease-of-use of composites involves the development of 'self-adhesive' composites that no longer require a separate adhesive to bond to tooth enamel/dentin. OBJECTIVES: To characterize the interfacial ultra-structure of an experimental self-adhesive filling material bonded to enamel and dentin using transmission electron microscopy (TEM). METHODS: The experimental self-adhesive material was bonded to bur-cut human enamel and dentin, and to fractured (smear-free) dentin, strictly according to the manufacturers' instructions. The specimens were stored for 1 day in distilled water (37 °C) prior to further common specimen processing for TEM. RESULTS: The experimental self-adhesive filling material revealed a typical micro-hybrid filler distribution. At bur-cut enamel, a tight interface was formed, mostly exhibiting only tiny micro-tags without distinct surface demineralization. At bur-cut dentin, the experimental self-adhesive filling material interacted superficially, with the surface structure being more irregular because of the bur preparation. No clear resin tags were formed due to the obstruction of dentin tubules with smear plugs. At fractured dentin, the formation of a relatively thin hybrid layer of maximum a few hundreds of nanometer was disclosed without clear surface demineralization. Distinct resin tags were formed due to the absence of smear plugs. Silver-nitrate infiltration showed a pattern of spot-like appearance of nano-leakage. Ag deposition was observed more along the dentin-adhesive interface of bur-cut dentin, as compared to that of fractured dentin. SIGNIFICANCE: The obtained tight interface at both enamel and dentin demonstrates the self-adhesive capacity of the experimental self-adhesive filling material.

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  • Factors related to prosthetic restoration in patients with shortened dental arches: a multicentre study

    K. Fueki, Y. Igarashi, Y. Maeda, K. Baba, K. Koyano, Y. Akagawa, K. Sasaki, T. Kuboki, S. Kasugai, N. R. Garrett

    JOURNAL OF ORAL REHABILITATION   38 ( 7 )   525 - 532   2011年7月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    P&gt;The aim of this study was to identify the factors related to prosthetic restoration in patients with shortened dental arches (SDA). SDA patients with 2-12 missing occlusal units were consecutively enrolled from seven university-based dental hospitals in Japan. Of the 145 subjects (mean age; 63 center dot 4 years), 41% chose no treatment and 59% sought to replace their edentulous spaces with removable partial dentures or implant-supported fixed partial dentures. Restoration decisions were related to tooth loss patterns. Only 3% of subjects missing just second molar(s) sought to receive prosthetic treatment, while the percentage increased to 58% in subjects who were missing first and second molars and 93% in subjects missing premolar(s). Logistic regression analyses found that young age, increased number of missing occlusal units, asymmetric arch and presence of chewing complaint were significant predictors for prosthetic restoration (P &lt; 0 center dot 05). Increased number of missing occlusal units and asymmetric arch were significant predictors for the presence of chewing complaint (P &lt; 0 center dot 05). These results suggest that perceived impairment of chewing ability owing to missing occlusal units is a critical factor for prosthetic restoration in SDA patients.

    DOI: 10.1111/j.1365-2842.2010.02183.x

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  • 補綴歯科治療の難易度を測定するプロトコルの信頼性と妥当性の検討

    木村 彩, 窪木 拓男, 市川 哲雄, 秀島 雅之, 佐藤 裕二, 玉置 勝司, 赤川 安正, 永尾 寛, 櫻井 薫, 馬場 一美, 皆木 省吾, 鱒見 進一, 佐藤 博信, 澤瀬 隆, 長岡 英一, 和気 裕之, 大山 喬史, 平井 敏博, 佐々木 啓一

    日本補綴歯科学会誌   3 ( 特別号 )   185 - 185   2011年5月

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    記述言語:日本語   出版者・発行元:(公社)日本補綴歯科学会  

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  • 固定性架工義歯学技工実習での視覚教材としての動画配信システム

    中島 隆, 水口 一, 新川重彦, 瀧内博也, 正木明日香, 三野卓哉, 笈田育尚, 木村 彩, 前川賢治, 窪木拓男

    岡山歯学会雑誌   30 ( 2 )   83 - 84   2011年

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  • 施設に入所している要介護高齢者の問題点を抽出するテュートリアル演習の試み.

    松香芳三, 縄稚久美子, 木村 彩, 完山 学, 水口 一, 三野卓哉, 丸濵功太郎, 前川賢治, 藤澤拓生, 園山 亘, 峯 篤史, 菊谷 武, 窪木拓男

    老年歯科医学   26 ( 1 )   36 - 45   2011年

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  • 顎関節症治療のための歯列矯正治療

    松香 芳三, 窪木 拓男

    コクラン・レビューアブストラクト   2011年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • 歯垢除去ならびに歯肉の健康における電動歯ブラシの相違

    松香 芳三, 窪木 拓男

    コクラン・レビューアブストラクト   2011年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • コラム「文献と臨床の橋わたし」 顎関節円板前方転位の自然経過.

    前川賢治, 窪木拓男

    日本歯科評論   71 ( 12 )   158 - 160   2011年

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  • コラム「文献と臨床の橋わたし」「生物学的見地から見た睡眠時ブラキシズムの現状と将来展望」.

    水口 一, 窪木拓男

    日本歯科評論   71 ( 11 )   172 - 174   2011年

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  • Enamel-Smear Compromises Bonding by Mild Self-Etch Adhesives

    A. Mine, J. De Munck, M. Vivan Cardoso, K. L. Van Landuyt, A. Poitevin, T. Kuboki, Y. Yoshida, K. Suzuki, B. Van Meerbeek

    JOURNAL OF DENTAL RESEARCH   89 ( 12 )   1505 - 1509   2010年12月

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    記述言語:英語   出版者・発行元:SAGE PUBLICATIONS INC  

    In light of the increased popularity of less acidic, so-called &apos;ultra-mild&apos; self-etch adhesives, adhesion to enamel is becoming more critical. It is hypothesized that this compromised enamel bonding should, to a certain extent, be attributed to interference of bur debris smeared across enamel during cavity preparation. High-resolution transmission electron microscopy revealed that the enamel smear layer differed not only in thickness, but also in crystal density and size, depending on the surface-preparation method used. Lab-demineralization of sections clearly disclosed that resin-infiltration of an ultra-mild self-etch adhesive progressed preferentially along micro-cracks that were abundantly present at and underneath the bur-cut enamel surface. The surface-preparation method significantly affected the nature of the smear layer and the interaction with the ultra-mild adhesive, being more uniform and dense for a lab-SiC-prepared surface vs. a clinically relevant bur-prepared surface.

    DOI: 10.1177/0022034510384871

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  • Basic position of the Japan Prosthodontic Society with respect to the policy statement on TMD by the American Association for Dental Research (AADR).

    Keiichi Sasaki, Hirofumi Yatani, Takuo Kuboki

    Journal of prosthodontic research   54 ( 4 )   151 - 2   2010年10月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.jpor.2010.07.004

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  • The effect of clinical experience on dentine bonding effectiveness: students versus trained dentists

    M. Ueda, A. Mine, J. De Munck, T. Hakogi, B. Van Meerbeek, T. Kuboki

    JOURNAL OF ORAL REHABILITATION   37 ( 9 )   653 - 657   2010年9月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    P&gt;Clinical successful application of dentine adhesives depends not only on material-related but also on operator-related factors. The purpose of this study was to evaluate the dentine bonding effectiveness of a self-etch composite cement applied by operators with or without clinical experience under well-standardized, randomized and blind conditions. Forty-eight bovine dentine surfaces were randomly divided into two groups. The first group consisted of eight dental students with no clinical experience at all, and the second group consisted of eight dentists with extensive experience in adhesive dentistry (mean experience of 11 center dot 4 years). Next, a 4-mm-diameter stainless steel rod (SUS-304) was bonded to the dentine surface using Panavia Fluoro cement (Kuraray Medical Inc., Tokyo, Japan). After application procedures, the specimens were randomized and shear bond-strength measurements were performed by a single blinded operator. Mann-Whitney U test was used to determine statistical differences in bond strength between the two groups, and Kruskal-Wallis was used to determine statistical difference between the student and dentist groups. The means and standard deviations of bond strength were 11 center dot 5 +/- 8 center dot 1 MPa for the student group and 7 center dot 1 +/- 4 center dot 3 MPa for the dentist group, respectively. The bond strength of the student group was significantly higher than that of the dentist group. However, the variability in bond strength was significantly higher in the student group, and some specimens failed prior to actual testing (included as 0 MPa). Clinical experience did not have a positive effect on the bonding effectiveness of the self-etch composite cement to dentine.

    DOI: 10.1111/j.1365-2842.2010.02095.x

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  • The Quasi-Three-Dimensional Marginal Leakage of Full-Coverage Crowns: Resin Coating Versus Sodium Hypochlorite Treatment

    Daizo Kawahara, Atsushi Mine, Jan De Munck, Takuo Kuboki, Yasuhiro Yoshida, Kazuomi Suzuki, Bart Van Meerbeek, Hirofumi Yatani

    INTERNATIONAL JOURNAL OF PROSTHODONTICS   23 ( 5 )   406 - 409   2010年9月

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    記述言語:英語   出版者・発行元:QUINTESSENCE PUBLISHING CO INC  

    This study compared the effects of various surface treatments and techniques on the marginal leakage of full-coverage crowns using a quasi-three-dimensional evaluation. Crowns were cast using a gold-silver-palladium alloy by means of the lost-wax technique. Twenty-eight recently extracted human molars were divided randomly into four groups according to surface treatment before crown cementation: (1) no pretreatment (negative control), (2) primer (positive control), (3) resin coating and primer, and (4) phosphoric acid, sodium hypochlorite, and primer. All specimens were cemented with composite cement. The lowest marginal leakage was observed in group 4. Variation in marginal leakage between specimens originating from the same tooth was observed. Int J Prosthodont 2010; 23: 406-409.

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  • Relationship between a chronically painful trapezius muscle and its metabolic state analyzed with PET/CT. 国際誌

    Satoru Takiguchi, Kenji Maekawa, Tsuyoshi Ono, Nobuya Sasai, Mitsumasa Kaji, Glenn T Clark, Takuo Kuboki

    Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics   110 ( 1 )   54 - 61   2010年7月

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    記述言語:英語   出版者・発行元:MOSBY-ELSEVIER  

    OBJECTIVES: The aim of this study was to investigate the intramuscular metabolic state in chronically painful muscles using positron-emission tomography/computerized tomography (PET/CT). STUDY DESIGN: The study included 140 consecutive noncancer subjects who underwent PET/CT screening for a physical checkup (mean age 56.0 +/- 10.22 y). The demographic data and information on pain in the neck/shoulder region were obtained using a questionnaire. The subjects who had an awareness of pain in the neck/shoulder region for >6 months were regarded to be pain subjects (n = 39). The minimal and maximal standardized uptake values (SUV) of [(18)F]fluorodeoxyglucose ((18)F-FDG) of the trapezius muscle in each subject were automatically calculated. RESULTS: The unpaired t test revealed that both the minimal and the maximal SUVs were significantly lower in the pain subjects than in the asymptomatic subjects. A multiple linear regression analysis also demonstrated a significant association between pain in the neck/shoulder region and the SUVs in the trapezius muscle. CONCLUSIONS: Uptake of (18)F-FDG was lower in the chronically painful trapezius muscle.

    DOI: 10.1016/j.tripleo.2010.02.028

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  • TEM characterization of a silorane composite bonded to enamel/dentin. 国際誌

    Atsushi Mine, Jan De Munck, Annelies Van Ende, Marcio Vivan Cardoso, Takuo Kuboki, Yasuhiro Yoshida, Bart Van Meerbeek

    Dental materials : official publication of the Academy of Dental Materials   26 ( 6 )   524 - 32   2010年6月

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    記述言語:英語   出版者・発行元:ELSEVIER SCI LTD  

    OBJECTIVES: The low-shrinking composite composed of combined siloxane-oxirane technology (Filtek Silorane, 3M ESPE, Seefeld, Germany) required the development of a specific adhesive (Silorane System Adhesive, 3M ESPE), in particular because of the high hydrophobicity of the silorane composite. The purpose of this study was to characterize the interfacial ultra-structure at enamel and dentin using transmission electron microscopy (TEM). METHODS: Non-demineralized/demineralized 70-90 nm sections were prepared following common TEM specimen processing procedures. RESULTS: TEM revealed a typical twofold build-up of the adhesive resin, resulting in a total adhesive layer thickness of 10-20 microm. At bur-cut enamel, a tight interface without distinct dissolution of hydroxyapatite was observed. At bur-cut dentin, a relatively thin hybrid layer of maximum a few hundreds of nanometer was formed without clear surface demineralization. No clear resin tags were formed. At fractured dentin, the interaction appeared very superficial (100-200 nm). Distinct resin tags were formed due to the absence of smear plugs. Silver-nitrate infiltration showed a varying pattern of both spot- and cluster-like appearance of nano-leakage. Traces of Ag were typically detected along some part of the enamel-adhesive interface and/or between the two adhesive resin layers. Substantially more Ag-infiltration was observed along the dentin-adhesive interface of bur-cut dentin, as compared to that of fractured dentin. CONCLUSIONS: The nano-interaction of Silorane System Adhesive should be attributed to its relatively high pH of 2.7. The obtained tight interface at both enamel and dentin indicates that the two-step self-etch adhesive effectively bridged the hydrophilic tooth substrate with the hydrophobic silorane composite.

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  • 周術期における歯科介入の重要性 岡山大学病院周術期管理センター歯科部門の取り組み

    丸山 貴之, 山中 玲子, 曽我 賢彦, 縄稚 久美子, 柳 文修, 兒玉 直紀, 中田 貴, 村田 尚道, 有岡 享子, 山根 美榮子, 三浦 留美, 羽川 操, 木南 美香, 高橋 明子, 住吉 由季子, 竹内 哲男, 窪木 拓男, 佐々木 朗, 森田 学

    日本口腔ケア学会雑誌   4 ( 1 )   83 - 83   2010年3月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔ケア学会  

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  • The effect of growth factors for bone augmentation to enable dental implant placement: A systematic review

    Kengo Shimono, Masamitsu Oshima, Hikaru Arakawa, Aya Kimura, Kumiko Nawachi, Takuo Kuboki

    Japanese Dental Science Review   46 ( 1 )   43 - 53   2010年2月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等  

    This systematic review assessed the potential benefits of growth factors for bone augmentation prior to the placement of dental implants in human. A systematic online review of the Medline database, using the PubMed search machine was performed between 1966 and November 2008 by entering the MeSH terms. The primary outcome of the included studies was bone regeneration of localized alveolar ridge defects. The initial search identified 119 papers from the electronic database. This review produced seven eligible papers that reported on bone augmentation with recombinant human Bone Morphogenetic Protein-2 (rhBMP-2), recombinant human Platelet-Derived Growth Factor (rhPDGF) and Plasma-Rich Growth Factor (PRGF). The rhBMP-2 affected local bone augmentation with increasing volume for higher doses. Both rhPDGF and PRGF showed a positive effect in favor of the growth factor. Differing levels and quantity of evidence were noted to be available for the growth factors evaluated, revealing that rhBMP-2, rhPDGF, and PRGF may stimulate local bone augmentation to various conditions. Especially the potential of rhBMP-2 is supportive. However, the confined number of investigators using these techniques and the low number of patient treatments reported in the literature, the generalizability of this approach is limited at this time. © 2009 Japanese Association for Dental Science.

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  • The relationship between temporomandibular joint pathosis and muscle tenderness in the orofacial and neck/shoulder region. 国際誌

    Eitoku Inoue, Kenji Maekawa, Hajime Minakuchi, Chiyomi Nagamatsu-Sakaguchi, Tsuyoshi Ono, Yoshizo Matsuka, Glenn T Clark, Takuo Kuboki

    Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics   109 ( 1 )   86 - 90   2010年1月

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    記述言語:英語   出版者・発行元:MOSBY-ELSEVIER  

    OBJECTIVE: The objective of this study was to investigate the association between TMJ pain/disk pathosis and the muscle tenderness pattern in the orofacial and neck/shoulder region. STUDY DESIGN: One hundred seventy-one TMD patients were divided into 4 groups, including group 1: patients with painful unilateral TMJ disk displacement (DD); group 2: patients with painless unilateral TMJ DD; group 3: patients with painless bilateral TMJ DD; and group 4: patients with a bilateral normal TMJ disk position (n = 41). Each subject underwent muscle palpation and the side-by-side number of muscle tenderness points was combined as the number of muscle tenderness points on each side. Within each group, DD with and without reduction subjects were separated into subgroups and then were analyzed. RESULTS: In group 1, the median muscle tenderness points on the side with painful TMJ DD without reduction was significantly higher than on the normal side (P = .019), whereas the palpation scores for painless DD patients showed no significant difference between the DD and normal sides. CONCLUSIONS: These results indicated painful disk displacement to possibly be correlated with ipsilateral muscle tenderness.

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  • Gene expression profile of mouse masseter muscle after repetitive electrical stimulation.

    Tsuyoshi Ono, Kenji Maekawa, Wataru Sonoyama, Shunji Kojima, Tsutomu Tanaka, Glenn T Clark, Takuo Kuboki

    Journal of prosthodontic research   54 ( 1 )   36 - 41   2010年1月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    PURPOSE: To examine gene expression profile changes in the mouse masseter muscle tissue after repetitive electrical stimulation by using a DNA microarray technique. METHODS: Nine male ICR mice aged 10 weeks were used. Each anesthetized mouse was secured on a platform in a supine position and the masseter muscle tissues on both sides were exposed. Bipolar electrodes were set on the right masseteric fascia to electrically stimulate the masseter muscle (8 V, 10 Hz, 20 ms) for 30 min. After cessation of stimulation bilateral masseter muscle tissues were sampled at 0 h (n=3), 1h (n=3), 2h (n=3). Total RNA was isolated from the homogenized muscle tissues and purified mRNA samples (50 microg) were processed and hybridized with microarray slides. Probe arrays were then scanned and analyzed to calculate the signal density. Gene expression profiles were compared at each time point between the right (stimulation side) and left (control side) masseter. When the gene expression levels were different more than 2-fold, the difference was regarded as positive. RESULTS: Of the 6400 genes assessed, 1733 genes were up-regulated and 515 genes were down-regulated in the stimulation side at least once during the experimental time course. These up- or down-regulated genes were associated with autoimmune/inflammatory disease (28/114), cardiovascular disease (17/61), neuroscience (12/50), apoptosis (27/93), diabetes/obesity (9/28), signal transduction (66/250) and others. 28 genes were up-regulated and 25 genes were down-regulated at all time points. CONCLUSIONS: Dramatic gene expression changes were induced by the repetitive electrical muscle stimulation in mouse masseter.

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  • 携帯電話を応用したEMAによる口腔インプラント一次手術後急性痛の測定における測定間隔の検討

    黒井 隆太, 水口 一, 川上 彩, 松香 芳三, 岡田 宏基, 窪木 拓男

    日本補綴歯科学会誌   2 ( 1 )   E29 - E29   2010年1月

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    記述言語:日本語   出版者・発行元:(公社)日本補綴歯科学会  

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  • 研究室から診療室へ State of the art, Prosthodontics 臨床家が知っておきたい補綴最新トピックス(第6回)未来の補綴歯科を切り開くために.

    窪木拓男

    補綴臨床   2010年

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  • 咬合崩壊を来した重度歯周炎患者に対する歯周・矯正・補綴専門医によるInterdisciplinary Approach

    完山学, 本城正, 高柴正悟, 藪中友絵, 山本悠美子, 藤田泰弘, 山城隆, 窪木拓男

    岡山歯学会雑誌   29 ( 1 )   55 - 60   2010年

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  • 地域の歯科医療関係者に対する支援 –口腔インプラント講習会と摂食・嚥下リハビリテーション教育–

    東 哲司, 有岡享子, 荒川 光, 江草正彦, 窪木拓男, 森田 学

    岡山医学会雑誌   121 ( 3 )   183 - 188   2010年

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  • (社)日本補綴歯科学会『歯の欠損の補綴歯科診療ガイドライン2008』の患者向けガイドライン作成に向けたペイシェントクエスチョンの収集-患者アンケートによる収集-

    石井 拓男, 窪木 拓男, 松香 芳三, 木村 彩

    厚生労働省科学研究費補助金(地域医療基盤開発推進事業)分担研究報告書   2010年

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  • 国際短期交換留学生ペアリング・システムの創成

    松尾 龍二, 山本 敏男, 皆木 省吾, 高柴 正悟, 佐々木 朗, 窪木 拓男, 永塚 仁, 松香 芳三, 白井 肇, 吉田 登志子

    平成21年度岡山大学学長裁量経費(教育研究プロジェクト等)成果報告書   2010年

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  • The quasi-three-dimensional marginal leakage of full-coverage crowns: Resin coating versus sodium hypochlorite treatment

    Daizo Kawahara, Atsushi Mine, Jan De Munck, Takuo Kuboki, Yasuhiro Yoshida, Kazuomi Suzuki, Bart Van Meerbeek, Hirofumi Yatani

    International Journal of Prosthodontics   23 ( 5 )   406 - 409   2010年

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    記述言語:英語  

    This study compared the effects of various surface treatments and techniques on the marginal leakage of full-coverage crowns using a quasi-three-dimensional evaluation. Crowns were cast using a gold-silver-palladium alloy by means of the lost-wax technique. Twenty-eight recently extracted human molars were divided randomly into four groups according to surface treatment before crown cementation: (1) no pretreatment (negative control), (2) primer (positive control), (3) resin coating and primer, and (4) phosphoric acid, sodium hypochlorite, and primer. All specimens were cemented with composite cement. The lowest marginal leakage was observed in group 4. Variation in marginal leakage between specimens originating from the same tooth was observed. © 2010 by Quintessence Publishing Co Inc.

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  • スプリント療法ガイドラインの確立

    皆木省吾, 築山能大, 有馬太郎, 市川哲雄, 窪木拓男, 兒玉直紀, 佐久間重光, 新谷明喜, 高津匡樹, 竹内哲男, 津賀一弘, 坪井明人, 中島啓一郎, 中野雅徳, 成田紀之, 波多野泰夫, 藤澤政紀, 船登雅彦, 鱒見進一, 松香芳三

    日本歯科医学会誌   2010年

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  • 卒前歯学臨床教育における学外インターンシップ実習の構築と今後の展望

    前川賢治, 窪木拓男, 伊澤俊次, 皆木省吾, 高柴正悟, 宮脇卓也, 江草正彦, 木村年秀, 山本敏男, 森田 学, 佐々木朗, 松尾龍二

    岡山歯科医学会雑誌   2010年

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  • 研究室から診療室へ State of the art, Prosthodontics 臨床家が知っておきたい補綴最新トピックス(第2回) 少数歯欠損における口腔インプラント治療

    荒川 光, 小田師巳, 窪木拓男

    補綴臨床   2010年

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  • A 15-year Clinical Comparative Study of the Cumulative Survival Rate of Cast Metal Core and Resin Core Restorations Luted with Adhesive Resin Cement

    Hikasa, Takayuki, Matsuka, Yoshizo, Mine, Atsushi, Minakuchi, Hajime, Hara, Emilio Satoshi, Van Meerbeek, Bart, Yatani, Hirofumi, Kuboki, Takuo

    International Journal of Prosthodontics   23 ( 5 )   2010年

  • 研究室から診療室へ State of the art, Prosthodontics 臨床家が知っておきたい補綴最新トピックス(第5回)顎関節症と補綴治療, 矯正治療.

    窪木拓男

    補綴臨床   2010年

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  • 研究室から診療室へ State of the art,Prosthodontics 臨床家が知っておきたい補綴最新トピックス(第3回)即時修復プロトコル、多数歯欠損と口腔インプラント治療

    窪木拓男, 完山 学, 笈田育尚, 上田明広

    補綴臨床   2010年

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  • Bonding effectiveness of two contemporary self-etch adhesives to enamel and dentin. 国際誌

    Atsushi Mine, Jan De Munck, Marcio V Cardoso, Kirsten L Van Landuyt, André Poitevin, Takuo Kuboki, Yasuhiro Yoshida, Kazuomi Suzuki, Paul Lambrechts, Bart Van Meerbeek

    Journal of dentistry   37 ( 11 )   872 - 83   2009年11月

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    記述言語:英語   出版者・発行元:ELSEVIER SCI LTD  

    OBJECTIVES: Among contemporary adhesives, self-etch adhesives have been adopted by general practitioners for routine adhesive restorative purposes, mainly because of their ease of use. However, many versions that differ for their clinical application procedure, pH, number of components, etc., are currently available on the market. The purpose of this study was to determine the bonding effectiveness of two new self-etch adhesives (Adper Easy Bond and Adper ScotchBond SE, 3M ESPE) to enamel and dentin using a micro-tensile bond strength (microTBS) protocol and to characterise the interfacial ultra-structure at enamel and dentin using transmission electron microscopy (TEM). METHODS: The adhesives were applied onto coronal human enamel and dentin surfaces and built up with the micro-hybrid resin composite Z100 (3M ESPE). The 'gold-standard' two-step self-etch adhesive Clearfil SE Bond (Kuraray) served as control. Specimens were sectioned to sticks and trimmed at the interface to a cylindrical hour-glass shape ('trimmed' micro-specimens). Non-demineralized and demineralized TEM sections through the adhesive-dentin/enamel interface were prepared by ultra-microtomy. RESULTS: The microTBS of the two self-etch adhesives to enamel was statistically significantly lower than that of the control. To dentin, the microTBS of Adper Easy Bond was significantly lower than that of Adper ScotchBond SE and the control. TEM showed a tight interface to enamel for all three self-etch adhesives. A relatively thick, completely demineralized and acid-resistant hybrid layer was formed at dentin by Adper ScotchBond SE, whereas the interaction of Adper Easy Bond was much shallower, and comparable to that of so-called 'ultra-mild' self-etch adhesives. Some degree of spot- and cluster-like nano-leakage was observed for both adhesives, but did not differ in extent and form from that observed for the control. CONCLUSIONS: Although the new two self-etch adhesives revealed a tight interaction at both enamel and dentin, their bond strength to both tooth tissues was generally lower than that of the control adhesive. Nevertheless, their bonding effectiveness appears in line with other simplified self-etch adhesives.

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  • 舌接触補助床(PAP)のガイドライン(案)

    植松 宏, 戸原 玄, 中島 純子, 菊谷 武, 高橋 浩二, 野原 幹司, 前田 芳信, 小野 高裕, 吉田 光由, 大野 友久, 佐々木 啓一, 高橋 裕, 田中 貴信, 鈴木 哲也, 谷口 尚, 小正 裕, 岡崎 定司, 津賀 一弘, 吉川 峰加, 西 恭宏, 飯沼 利光, 川良 美佐雄, 皆木 省吾, 古屋 純一, 小山 重人, 木本 統, 飯島 守雄, 乙丸 貴史, 隅田 由香, 猪原 健, 中根 綾子, 若杉 葉子, 大内 ゆかり, 都島 千明, 田村 文誉, 尾澤 昌悟, 吉岡 文, 堀 一浩, 城下 尚子, 洲脇 道弘, 難波 謙介, 清水 博史, 津江 文武, 今井崎 太一, 加地 彰人, 長岡 英一, 佐藤 裕二, 高井 良招, 赤川 安正, 井上 農夫男, 服部 正己, 森戸 光彦, 北川 昇, 窪木 拓男, 藤澤 政紀, 玉置 勝司, 築山 能大, 永尾 寛, 萩原 芳幸, 松香 芳三, 宮城 敦, 重枝 昭広, 中村 全宏, 日本老年歯科医学会

    老年歯科医学   24 ( 2 )   104 - 116   2009年9月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

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  • 携帯電話を用いたEMAシステムの歯科領域への応用の試み

    黒井 隆太, 水口 一, 坂口 千代美, 川上 彩, 松香 芳三, 岡田 宏基, 窪木 拓男

    岡山歯学会雑誌   28 ( 1 )   87 - 87   2009年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • Polyphosphoric acid treatment promotes bone regeneration around titanium implants

    K. Maekawa, K. Shimono, M. Oshima, Y. Yoshida, B. Van Meerbeek, K. Suzuki, T. Kuboki

    JOURNAL OF ORAL REHABILITATION   36 ( 5 )   362 - 367   2009年5月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    This study was conducted to evaluate the effect of polyphosphoric acid (PPA) treatment on bone regeneration around titanium (Ti) implants in vivo. Adsorption of PPA by Ti was achieved by immersing Ti implants (2 mm in diameter, 4 mm in length) in different concentrations of PPA solution (0, 1 and 10 wt%) for 24 h at 37 degrees C after proper Ti surface cleaning. The treated Ti implants were implanted on 8-week-old-male rat (n = 30) tibiae. Two or four weeks after implantation, all animals were deeply anaesthetized and underwent perfusion fixation. Ten specimens in each condition were further immersed in the same fixative for 1 week and eventually embedded in polyester resin. Afterwards, undecalcified sections were ground to a thickness of approximately 70 mu m parallel to the long axis of the implant. The sections were stained with basic fuchsine and methylene blue and then examined by light microscopy. For quantitative evaluation of bone regeneration around the implants, the bone-implant contact ratio (BICR) was determined. Polyphosphoric acid treatment of the Ti implant surface significantly enhanced direct bone contact to the Ti surface. Especially, the BICRs of the 1 wt% PPA-treated Ti implants were significantly higher than those of the control untreated Ti implants, both 2 and 4 weeks after implantation. At 4 weeks, 10 wt% PPA-treated implants also significantly increased the BICR as compared to that of the untreated Ti implants. These results suggest that PPA treatment promotes osteoconductivity of Ti in vivo.

    DOI: 10.1111/j.1365-2842.2008.01928.x

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  • BOTULINUM TOXIN TYPE A (150 kDa) DECREASES EXAGGERATED NEUROTRANSMITTER RELEASE FROM TRIGEMINAL GANGLION NEURONS AND RELIEVES NEUROPATHY BEHAVIORS INDUCED BY INFRAORBITAL NERVE CONSTRICTION

    Y. Kitamura, Y. Matsuka, I. Spigelman, Y. Ishihara, Y. Yamamoto, W. Sonoyama, T. Kuboki, K. Oguma

    NEUROSCIENCE   159 ( 4 )   1422 - 1429   2009年4月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Many patients with trigeminal neuropathies suffer severe chronic pain which is inadequately alleviated with centrally-acting drugs. These drugs also possess severe side effects making compliance difficult. One strategy is to develop new treatments without central side effects by targeting peripheral sensory neurons, since sensory neuron excitability and neurotransmitter release increase in chronic pain states. Such treatments may include the highly purified botulinum toxin type A 150 kDa (BoNT/A) which reportedly blocks vesicular neurotransmitter release. We set out to determine if experimental trigeminal neuropathy induced by infraorbital nerve constriction (IoNC) in rats could alter neurotransmitter release from somata of trigeminal sensory neurons and if it could be aften-uated by BoNT/A. Thus, we monitored the secretory activity of acutely dissociated trigeminal ganglion (TRG) neurons from naive and IoNC rats by measuring the fluorescence intensity of the membrane-uptake marker (N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium dibromide (FM4-64). FM4-64 staining showed that neurons possess a pool of recycled vesicles which could be released by high KCl (75 mM) application. BoNT/A pre-treatment of acutely dissociated TRG neurons from naive rats significantly reduced the rate of FM4-64 dye release. Neurons isolated from TRG ipsilateral to IoNC exhibited significantly faster onset of FM4-64 release than neurons contralateral to

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  • Simvastatin induces the odontogenic differentiation of human dental pulp stem cells in vitro and in vivo. 国際誌

    Yosuke Okamoto, Wataru Sonoyama, Mitsuaki Ono, Kentaro Akiyama, Takuo Fujisawa, Masamitsu Oshima, Yohei Tsuchimoto, Yoshizo Matsuka, Tatsuji Yasuda, Songtao Shi, Takuo Kuboki

    Journal of endodontics   35 ( 3 )   367 - 72   2009年3月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    Statin, 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, is known to promote bone formation. However, it is not clear whether statin affects the differentiation of pulp cells. This study used a cell proliferation assay, cell cycle analysis, quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and in vivo transplantation to examine the effects of simvastatin on human dental pulp stem cells (DPSCs) in vitro and in vivo. Simvastatin at 1 mumol/L was able to significantly suppress the proliferation of DPSCs without inducing apoptosis. Quantitative RT-PCR revealed both osteocalcin and dentin sialophosphoprotein to be significantly up-regulated when DPSCs were cultured with simvastatin in comparison to bone morphogenetic protein-2 treatment. The in vivo transplantation data showed that simvastatin treatment promoted mineralized tissue formation. Taken together, these results suggest that statin might be an ideal active ingredient to accelerate the differentiation of DPSCs.

    DOI: 10.1016/j.joen.2008.11.024

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  • CCN family 2/connective tissue growth factor modulates BMP signalling as a signal conductor, which action regulates the proliferation and differentiation of chondrocytes. 国際誌

    Azusa Maeda, Takashi Nishida, Eriko Aoyama, Satoshi Kubota, Karen M Lyons, Takuo Kuboki, Masaharu Takigawa

    Journal of biochemistry   145 ( 2 )   207 - 16   2009年2月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Both CCN family 2/connective tissue growth factor (CCN2/CTGF) and bone morphogenetic protein (BMP)-2 play an important role in cartilage metabolism. We evaluated whether or not CCN2 would interact with BMP-2, and examined the combination effect of CCN2 with BMP-2 (CCN2-BMP-2) on the proliferation and differentiation of chondrocytes. Immunoprecipitation-western blotting analysis, solid-phase binding assay and surface plasmon resonance (SPR) spectroscopy showed that CCN2 directly interacted with BMP-2 with a dissociation constant of 0.77 nM as evaluated by SPR. An in vivo study revealed that CCN2 was co-localized with BMP-2 at the pre-hypertrophic region in the E18.5 mouse growth plate. Interestingly, CCN2-BMP-2 did not affect the BMP-2/CCN2-induced phosphorylation of p38 MAPK but caused less phosphorylation of ERK1/2 in cultured chondrocytes. Consistent with these results, cell proliferation assay showed that CCN2-BMP-2 stimulated cell growth to a lesser degree than by either CCN2 or BMP-2 alone, whereas the expression of chondrocyte marker genes and proteoglycan synthesis, representing the mature chondrocytic phenotype, was increased collaboratively by CCN2-BMP-2 treatment in cultured chondrocytes. These findings suggest that CCN2 may regulate the proliferating and differentiation of chondrocytes by forming a complex with BMP-2 as a novel modulator of BMP signalling.

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  • 携帯電話を用いたEMAシステムの歯科領域への応用の試み

    黒井 隆太, 水口 一, 坂口 千代美, 川上 彩, 松香 芳三, 岡田 宏基, 窪木 拓男

    日本補綴歯科学会誌   1 ( 1 )   E10 - E10   2009年1月

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    記述言語:日本語   出版者・発行元:(公社)日本補綴歯科学会  

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  • 臨床科が知っておきたい補綴最新トピックス 第1回 補綴臨床における専門性

    窪木拓男

    補綴臨床   42 ( 6 )   660 - 667   2009年

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  • 三叉神経痛治療のための薬物治療法の開発

    松香芳三, 北村洋一, 山本由弥子, 園山 亘, Spigelman I, 小熊恵二, 窪木拓男

    日本歯科医学会誌   28 ( 1 )   49 - 53   2009年

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  • 咀嚼能力評価の標準化に関する研究

    市川哲雄, 永尾 寛, 窪木拓男, 木村 彩, 秀島雅之, 玉置勝司, 小平順可, 貞森紳至, 津賀一弘, 越野 寿, 赤川安正, 平井敏博

    日本歯科医学会誌   28 ( 1 )   34 - 38   2009年

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  • 病診連携下に腸骨移植による顎骨増量術と歯科インプラント治療を行った3症例 —咬合機能回復後の5年以上経過症例—

    加納みわ, 植野高章, 脇本真理, 白数信明, 太田晃子, 沢木聖子, 森谷徳文, 山近英樹, 松村達志, 山田朋弘, 三島克章, 窪木拓男, 緒方憲一郎, 佐野和生

    岡山歯学会雑誌   28 ( 1 )   71 - 74   2009年

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  • 歯科PBL教育の効果に対するチューターの経験度の影響

    松香芳三, 礒兼衣里, 完山 学, 水口 一, 上原淳二, 縄稚久美子, 上枝麻友, 笈田育尚, 三野卓哉, 新川重彦, 前川賢治, 荒川 光, 園山 亘, 窪木拓男

    岡山歯学会雑誌   28 ( 1 )   11 - 17   2009年

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  • 岡山大学病院周術期管理センター(歯科部門)設立後5ヶ月間の活動内容および今後の展開

    山中玲子, 曽我賢彦, 縄稚久美子, 柳 文修, 兒玉直紀, 中田 貴, 三浦留美, 羽川 操, 竹内哲男, 山根美栄子, 森田 学, 高柴正悟, 浅海淳一, 皆木省吾, 吉山昌宏, 下野 勉, 窪木拓男, 佐々木朗, 森田 潔

    岡山歯学会雑誌   28 ( 1 )   37 - 42   2009年

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  • 超高気孔率ハイドロキシアパタイト(アパセラムAX)の骨増量術への使用経験

    植野高章, 太田晃子, 白数信明, 加納みわ, 脇本真理, 森谷徳文, 水川展吉, 山近英樹, 山田朋弘, 松村達志, 窪木拓男, 佐野和生, 玉村 亮, 長塚 仁

    岡山歯学会雑誌   28 ( 1 )   65 - 69   2009年

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  • 安静時ヒト咬筋血流動態に対する直線偏光近赤外線照射の影響

    瀧口 悟, 前川賢治, 小野 剛, 窪木拓男

    日本顎関節学会雑誌   21 ( 2 )   105 - 109   2009年

  • 岡山大学歯学部戦略的計画 -求められている今後の対応策-

    松香芳三, 池亀美華, 吉田登志子, 有馬太郎, 皆木省吾, 山本敏男, 高柴正悟, 窪木拓男, 北山滋雄, 滝川正春, 松尾龍二

    岡山歯学会雑誌   28 ( 2 )   123 - 130   2009年

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  • 家族の介護により経口摂取が可能となり、胃瘻から脱却した症例

    松香芳三, 笈田育尚, 熊田 愛, 縄稚久美子, 西山憲行, 菊谷 武, 窪木拓男

    老年歯科医学会雑誌   24 ( 2 )   91 - 96   2009年

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  • QDT発刊400号記念特集 現在, そしてこれからの補綴を考える: 第1部 補綴装置別: これからの補綴はどう変わっていくか? (1)クラウン・ブリッジ編

    窪木拓男

    QDT   34 ( 4 )   14 - 25   2009年

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  • 咬合違和感への対応—咬合感覚異常症の疾患概念確立に向けて

    窪木拓男

    Dental Diamond   34 ( 486 )   62 - 67   2009年

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  • 岡山大学病院NST専門療法士研修プログラムにおける口腔ケア実習の試み

    縄稚久美子, 住吉由季子, 三浦留美, 野口絢子, 有岡享子, 石田 瞭, 皆木省吾, 内藤 稔, 伊野英男, 坂本八千代, 松香芳三, 窪木拓男

    歯界展望特別号「めざせ! 健・口・美 未来に向けた歯科医療 第21回日本歯科医学会総会」   特別号   270   2009年

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  • 大腸菌由来・遺伝子組換BMP-2と塩基性ゼラチンを用いた骨補填材の効果

    下野賢吾, 大島正充, 完山 学, 大野充昭, 縄稚久美子, 田畑泰彦, Sebald W, 洲鎌和茂, 窪木拓男

    歯界展望特別号「めざせ! 健・口・美 未来に向けた歯科医療 第21回日本歯科医学会総会」   特別号   296   2009年

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  • ポリリン酸処理を用いたチタンインプラントの高機能化

    水口 一, 坂口千代美, 川上 彩, 黒井隆太, 松香芳三, Clark GT, 窪木拓男

    歯界展望特別号「めざせ! 健・口・美 未来に向けた歯科医療 第21回日本歯科医学会総会」   特別号   295   2009年

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  • 小型貼付型ブラキシズム検出装置の歯科臨床への応用

    水口 一, 坂口千代美, 川上 彩, 黒井隆太, 松香芳三, Clark GT, 窪木拓男

    歯界展望特別号「めざせ! 健・口・美 未来に向けた歯科医療 第21回日本歯科医学会総会」   特別号   223   2009年

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  • Bonding effectiveness and interfacial characterization of a HEMA/TEGDMA-free three-step etch&rinse adhesive. 国際誌

    Atsushi Mine, Jan De Munck, Kirsten L Van Landuyt, André Poitevin, Takuo Kuboki, Yasuhiro Yoshida, Kazuomi Suzuki, Paul Lambrechts, Bart Van Meerbeek

    Journal of dentistry   36 ( 10 )   767 - 73   2008年10月

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    記述言語:英語   出版者・発行元:ELSEVIER SCI LTD  

    OBJECTIVES: Small and hydrophilic monomers as HEMA and TEGDMA can easily penetrate human tissues. For biocompatibility issues it is therefore better to avoid such monomers in dental adhesive formulations. The purpose of this study was (1) to determine the micro-tensile bond strength (muTBS) to enamel/dentin of a HEMA/TEGDMA-free three-step etch&rinse adhesive (cmf Adhesive System, Saremco), and (2) to characterize it's interfacial interaction with enamel/dentin using transmission electron microscopy (TEM). METHODS: Twelve intact freshly extracted human third molars were used for muTBS. The three-step etch&rinse adhesive OptiBond FL (Kerr) served as control adhesive. After bonding procedures and subsequent composite build-up (Z100, 3M ESPE), specimens were sectioned to sticks and trimmed at the interface to a cylindrical hour-glass shape. TEM section of non-demineralized and demineralized sections were prepared by ultra-microtomy and examined. RESULTS: The muTBS of the cmf Adhesive System to enamel was not statistically significantly different from that of the control (OptiBond FL) adhesive (p=0.3512); the muTBS to dentin was however significantly lower (p<0.0001). TEM showed a tight interface at enamel for both adhesives. A relatively thick, completely demineralized and acid-resistant hybrid layer was formed at dentin. Some degree of spot- and cluster-like nano-leakage was observed with cmf Adhesive System, but did not differ in extent and form in comparison to that observed with the OptiBond FL control. CONCLUSIONS: The new three-step adhesive cmf Adhesive System showed indications of good bonding effectiveness, certainly to enamel and only significantly lower to dentin, when compared to the gold-standard adhesive. The latter may be related to the adapted HEMA/TEGDMA-free formulation and the somewhat lower mechanical properties.

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  • 高齢者の歯列欠損補綴治療法に関するチュートリアル学習とその効果

    前川 賢治, 前田 あずさ, 内部 健太, 北村 洋一, 暈 貴行, 上原 淳二, 完山 学, 縄稚 久美子, 荒川 光, 藤沢 拓生, 水口 一, 峯 篤史, 松香 芳三, 窪木 拓男

    日本歯科医学教育学会雑誌   24 ( 2 )   207 - 213   2008年8月

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    記述言語:日本語   出版者・発行元:(一社)日本歯科医学教育学会  

    われわれの教室では、高齢者の歯列欠損補綴に際し、欠損や口腔内の問題により患者がどのような障害をきたしているかを理解し、個々の患者の全身状態、高齢者特有の社会的背景、精神的・心理的特徴を考慮に入れた臨床決断を行うための思考能力を開発することを目標としたチュートリアル教育を行った。対象は、平成17年度岡山大学歯学部4年次生57名である。チュートリアル演習を行うため、これらの学生を少人数のグループ(7〜8名)に分けた。次に、グループ単位で歯列欠損を有する高齢患者の診療を見学させ、医療面接によりその患者に生じている医学的、精神・社会的な問題や苦悩について実際に質問、抽出させた。この患者見学を起点とし、本患者の問題点や苦悩を解決するための方策について、270分×5回の問題発見解決型チュートリアル演習を行った。毎回の学習後に各グループ構成員に対して本学習の行動目標に当たる9項目:思考能力(4項目)、分析力(1項目)、概念を創出する力(2項目)、学習態度(2項目)について4段階で評価し、各項目の中央値の経時的変化を本学習の効果として評価した。その結果、評価した9項目すべてにおいて、結果の中央値が演習最終日までの期間に有意に上昇し、個々の患者の全身状態、高齢者特有の社会的背景、精神的・心理的特徴を考慮に入れた臨床決断を行うという本学習目標に対応して教育効果が得られたことが示された。(著者抄録)

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  • 高齢者の歯列欠損補綴治療法に関するチュートリアル学習とその効果

    前川 賢治, 前田 あずさ, 内部 健太, 北村 洋一, 暈 貴行, 上原 淳二, 完山 学, 縄稚 久美子, 荒川 光, 藤沢 拓生, 水口 一, 峯 篤史, 松香 芳三, 窪木 拓男

    日本歯科医学教育学会雑誌   24 ( 2 )   207 - 213   2008年8月

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    記述言語:日本語   出版者・発行元:(一社)日本歯科医学教育学会  

    われわれの教室では、高齢者の歯列欠損補綴に際し、欠損や口腔内の問題により患者がどのような障害をきたしているかを理解し、個々の患者の全身状態、高齢者特有の社会的背景、精神的・心理的特徴を考慮に入れた臨床決断を行うための思考能力を開発することを目標としたチュートリアル教育を行った。対象は、平成17年度岡山大学歯学部4年次生57名である。チュートリアル演習を行うため、これらの学生を少人数のグループ(7〜8名)に分けた。次に、グループ単位で歯列欠損を有する高齢患者の診療を見学させ、医療面接によりその患者に生じている医学的、精神・社会的な問題や苦悩について実際に質問、抽出させた。この患者見学を起点とし、本患者の問題点や苦悩を解決するための方策について、270分×5回の問題発見解決型チュートリアル演習を行った。毎回の学習後に各グループ構成員に対して本学習の行動目標に当たる9項目:思考能力(4項目)、分析力(1項目)、概念を創出する力(2項目)、学習態度(2項目)について4段階で評価し、各項目の中央値の経時的変化を本学習の効果として評価した。その結果、評価した9項目すべてにおいて、結果の中央値が演習最終日までの期間に有意に上昇し、個々の患者の全身状態、高齢者特有の社会的背景、精神的・心理的特徴を考慮に入れた臨床決断を行うという本学習目標に対応して教育効果が得られたことが示された。(著者抄録)

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  • Relationship between the frequency of sleep bruxism and the prevalence of signs and symptoms of temporomandibular disorders in an adolescent population

    Chiyomi Nagamatsu-Sakaguchi, Hajime Minakuchi, Glenn T. Clark, Takuo Kuboki

    INTERNATIONAL JOURNAL OF PROSTHODONTICS   21 ( 4 )   292 - 298   2008年7月

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    記述言語:英語   出版者・発行元:QUINTESSENCE PUBLISHING CO INC  

    Purpose: The relationship between sleep bruxism (SB) and temporomandibular disorders (TMD) is unclear. This study aimed to estimate SB prevalence in an adolescent population and to investigate the relationship between SB frequency and prevalence of TMD signs/symptoms. Materials and Methods: First-year students at a high school in Okayama, Japan, were recruited in 2005, with 195 subjects responding. The SB detection device was a miniature disposable device (BiteStrip, SLP) that indicated the total SB events per night on a 4-grade score. The subjects were divided into severe and nonsevere SB groups with SB frequency cutoffs. The subjects were examined for temporomandibular joint (TMJ) noise during mouth opening/closing, tenderness of the masticatory/cervical muscles, and range of TMJ condylar movement. The presence/absence of headache and shoulder stiffness was also determined by the interview. The odds ratio (OR) and confidence interval (CI) were calculated to test the relationship between SB frequency, gender difference, and presence of the TMD signs/symptoms by multiple regression analysis. Results: Severe SB (more than 125 events per night) was significantly related to the presence of TMJ clicking (OR: 3.74, CI: 1.22-11.49, P =.02), while gender (male) was not related to the presence of TMJ clicking. Severe SB was not related to headache, though gender (male) was significantly related to headache (OR: 2.52, CI: 1.04-6.11, P = .04) in these subjects. Conclusion: These results suggest that the presence of TMJ clicking was closely related to severe SB in an adolescent population.

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  • CCN family 2/connective tissue growth factor (CCN2/CTGF) stimulates proliferation and differentiation of auricular chondrocytes

    T. Fujisawa, T. Hattori, M. Ono, J. Uehara, S. Kubota, T. Kuboki, M. Takigawa

    OSTEOARTHRITIS AND CARTILAGE   16 ( 7 )   787 - 795   2008年7月

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    記述言語:英語   出版者・発行元:W B SAUNDERS CO LTD  

    Objectives: CCN family 2/connective tissue growth factor (CCN2/CTGF) is an atypical growth factor for growth plate chondrocytes. It plays an important role in their proliferation and differentiation in vitro, but does not stimulate hypertrophy or calcification of articular chondrocytes. We herein report for the first time that CCN2/CTGF promotes growth and differentiation of auricular chondrocytes and maintains their molecular phenotype in vitro and in vivo.
    Methods: Auricular chondrocytes were isolated from rabbit auricular cartilage by trypsin-collagenase treatment, and treated with human recombinant CCN2/CTGF or infected with adenovirus harboring the ccn2/ctgf gene. Cell proliferation was measured by [3 H] thymidine incorporation and MTS assay, and changes in gene expression of auricular chondrocyte markers were monitored by real-time polymerase chain reaction, Northern hybridization, and histological analysis. For in vivo studies, auricular chondrocytes were cultured as pellets and implanted subcutaneously after treatment of recombinant human CCN2/CTGF. Ectopically formed cartilage was subjected to histological analysis. Cell death was monitored by in situ TUNEL analysis.
    Results: CCN2/CTGF stimulated proliferation, differentiation and synthesis of elastin and proteoglycans of rabbit primary auricular chondrocytes in a dose-dependent manner. CCN2/CTGF caused a 2.5-fold increase in the expression of elastin in comparison to the control, resulting in enhanced deposition of elastin fibers in a monolayer culture of auricular chondrocytes. Mineralization was not induced; in contrast, CCN2/CTGF stimulated expression of matrix gla protein which is known to impair mineralization. Furthermore, pretreatment of pellets of auricular chondrocytes with CCN2/CTGF and subcutaneous implantation significantly enhanced the growth of ectopic auricular cartilage pieces expressing phenotypic markers of auricular chondrocytes including type 11 and X collagen. Notably, chondrocyte apoptosis was impaired by CCN2/CTGF.
    Conclusions: These findings show that CCN2/CTGF may be a suitable agent for promoting differentiation and growth of auricular chondrocytes, while preventing mineralization and apoptosis, and suggests that CCN2/CTGF may be useful for the repair or reconstruction Of Elastic cartilage. (C) 2007 Ostecarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.joca.2007.11.001

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  • The effect of peripheral administration of botulinum toxin type A in rats with neuropathic pain

    Yoshizo Matsuka, Yoichi Kitamura, Igor Spigelman, Rahena Akther, Yumiko Yamamoto, Wataru Sonoyama, Keiji Oguma, Takuo Kuboki

    TOXICON   51   7 - 7   2008年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    DOI: 10.1016/j.toxicon.2008.04.020

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  • Novel transcription-factor-like function of human matrix metalloproteinase 3 regulating the CTGF/CCN2 gene. 国際誌

    Takanori Eguchi, Satoshi Kubota, Kazumi Kawata, Yoshiki Mukudai, Junji Uehara, Toshihiro Ohgawara, Soichiro Ibaragi, Akira Sasaki, Takuo Kuboki, Masaharu Takigawa

    Molecular and cellular biology   28 ( 7 )   2391 - 413   2008年4月

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    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    Matrix metalloproteinase 3 (MMP3) is well known as a secretory endopeptidase that degrades extracellular matrices. Recent reports indicated the presence of MMPs in the nucleus (A. J. Kwon et al., FASEB J. 18:690-692, 2004); however, its function has not been well investigated. Here, we report a novel function of human nuclear MMP3 as a trans regulator of connective tissue growth factor (CCN2/CTGF). Initially, we cloned MMP3 cDNA as a DNA-binding factor for the CCN2/CTGF gene. An interaction between MMP3 and transcription enhancer dominant in chondrocytes (TRENDIC) in the CCN2/CTGF promoter was confirmed by a gel shift assay and chromatin immunoprecipitation. The CCN2/CTGF promoter was activated by overexpressed MMP3, whereas a TRENDIC mutant promoter lost the response. Also, the knocking down of MMP3 suppressed CCN2/CTGF expression. By cytochemical and histochemical analyses, MMP3 was detected in the nuclei of chondrocytic cells in culture and also in the nuclei of normal and osteoarthritic chondrocytes in vivo. The nuclear translocation of externally added recombinant MMP3 and six putative nuclear localization signals in MMP3 also were shown. Furthermore, we determined that heterochromatin protein gamma coordinately regulates CCN2/CTGF by interacting with MMP3. The involvement of this novel role of MMP3 in the development, tissue remodeling, and pathology of arthritic diseases through CCN2/CTGF regulation thus is suggested.

    DOI: 10.1128/MCB.01288-07

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  • Effect of polyphosphoric acid pre-treatment of titanium on attachment, proliferation, and differentiation of osteoblast-like cells (MC3T3-E1). 国際誌

    Kenji Maekawa, Yasuhiro Yoshida, Atsushi Mine, Bart van Meerbeek, Kazuomi Suzuki, Takuo Kuboki

    Clinical oral implants research   19 ( 3 )   320 - 5   2008年3月

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING  

    PURPOSE: To evaluate the effect of polyphosphoric acid (PPA) pre-treatment of titanium (Ti) on the initial attachment, proliferation, and differentiation of mouse osteoblast-like cells (MC3T3-E1). MATERIALS AND METHODS: Adsorption of PPA to Ti was achieved by immersing Ti disks (15 mm in diameter) into 0, 1, and 10 wt% PPA and 10 wt% orthophosphoric acid (OPA) for 24 h. On each pre-treated Ti disk, 5.0 x 10(4) MC3T3-E1 cells were seeded, and 1, 3, and 5 h later cell attachment was evaluated. Cell proliferation was also determined 1, 3, and 5 days after cell seed. Cell differentiation was evaluated 5, 10, and 15 days after cell seed using osteoblast marker gene expression. Total RNA was purified from each culture and Type-I collagen, alkaline phosphatase, and osteocalcin mRNA expression levels were measured by real-time reverse transcription polymerase chain reaction. RESULTS: Adsorption of PPA or OPA to Ti significantly accelerated initial cell attachment at every time point (P<0.0001). As with cell attachment, cell proliferation was also accelerated on the PPA-treated Ti disks in a dose-dependent manner at every time point (P<0.0001). However, OPA-treated Ti disks did not enhance the cell proliferation. Regarding osteoblastic differentiation, PPA-treated Ti significantly accelerated the Type-I collagen gene expression at 5 and 10 days after cell seed. Regarding alkaline phosphatase and osteocalcin gene expression, no significant difference was found among the different Ti surface conditions. CONCLUSION: The accelerated cell behavior following Ti pre-treatment with PPA is a promising new strategy to fabricate bioactive Ti implants.

    DOI: 10.1111/j.1600-0501.2007.01477.x

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  • Dental implant treatment for a patient with bilateral cleft lip and palate

    Masako Sawaki, Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Kozo Honda, Nobuaki Shirasu, Takno Kuboki, Toshio Sugahara

    ACTA MEDICA OKAYAMA   62 ( 1 )   59 - 62   2008年2月

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    記述言語:英語   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    Dental reconstruction in the cleft space is difficult in some patients with cleft lip and palate because of oronasal fistulas. Most of these patients receive a particle cancellous bone marrow (PCBM) graft to close the alveolar cleft, and secondary bone grafting is also required. Treatment options for the alveolar cleft including fixed or removable prostheses require the preparation of healthy teeth and are associated with functional or social difficulties. Recently, the effectiveness of dental implant treatment for cleft lip and palate patients has been reported. However, there have been few reports on the use of this treatment in bilateral cleft lip and palate patients. We report the case of a patient who had bilateral cleft lip and palate and was missing both lateral incisors. She received dental implant treatment after a PCBM graft and ramus bone onlay grafting (RBOG). A 34-month postoperative course was uneventful.

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  • 骨補填材と自家骨移植を用いた顎骨増量後に即時荷重インプラント治療を行った1例

    太田晃子, 植野高章, 緒方憲一郎, 白数信明, 高木慎, 窪木拓男, 菅原利夫

    日本口腔インプラント学会誌   21 ( 1 )   207 - 208   2008年

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  • 欠損歯を補うための介入:総義歯の咬合面が及ぼす影響について

    窪木 拓男, 松香 芳三

    コクラン・レビューアブストラクト   2008年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • ボツリヌス毒素由来のポリペプチドを有効成分として含む鎮痛作用を有する医薬品

    窪木 拓男, 松香 芳三, 小熊 恵一, 北村 洋一

    岡山TLO技術シーズ集   10 - 10   2008年

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    記述言語:日本語  

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  • 【審美歯科・インプラントワードブック】 ベーシック補綴 全身とQOL 精神医学的分類

    松香 芳三, 窪木 拓男

    補綴臨床   140 - 141   2008年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • 高度萎縮上顎骨に腸骨移植を行ないコンピュータガイドシステム(ノーベルガイド)により即時荷重を行なったインプラント治療の1症例

    植野高章, 脇本真理, 白数信明, 本多浩三, 水川展吉, 窪木拓男, 菅原利夫

    岡山歯学会雑誌   27 ( 1 )   69   2008年

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  • 歯学部通信(2008年版)

    窪木拓男

    岡山大学歯学部同窓会誌   2008年

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  • 専用ホームページならびにインターネット掲示板を利用した補綴スキル教育における学生・教官相互コミュニケーション

    暈貴行, 水口一, 内部健太, 北村洋一, 前田あずさ, 永松(坂口, 千代美, 上原淳二, 松香芳三, 窪木拓男

    岡山歯学会雑誌   27 ( 1 )   23 - 28   2008年

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  • EBMワークショップと大学院教育の実質化

    松香芳三, 福岡敏雄, 完山 学, 前川賢治, 荒川 光, 水口 一, 園山 亘, 上原淳二, 縄稚久美子, 山崎聖也, 窪木拓男, 滝川正春, 松尾龍二, 田中紀章

    岡山歯学会雑誌   27 ( 1 )   43 - 49   2008年

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  • レスポンスシフトが歯科治療に際したQOL測定に与える影響.

    木村 彩, 荒川 光, 窪木拓男

    NPO法人健康医療評価研究機構 iHope Newsletter   ( 20号 )   1 - 3   2008年

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  • 【Multiple problemsの治療戦略】 内科診療におけるmultiple problems 【薬物投与のジレンマ】 抗凝固薬,抗血小板薬投与中の患者が抜歯を必要とする場合.

    宮脇卓也, 窪木拓男

    Medicina   45 ( 9 )   1618 - 1621   2008年

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  • 医療系大学院を魅力的な教育現場に再生するために―大学院教育の実質化とポストEBM教育―

    窪木拓男

    EBMジャーナル   9 ( 5 )   634 - 639   2008年

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  • 食べる知恵と全身健康.―生涯健康においしく食べるためのお話です―

    窪木拓男

    Vision   16   2008年

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  • 学生諸君大志を抱け―研究の勧め.

    窪木拓男

    岡山大学歯学部広報   3   2008年

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  • Responses of the hypothalamic-pituitary-adrenal axis and pain threshold changes in the orofacial region upon cold pressor stimulation in normal volunteers. 国際誌

    Koji Suzuki, Kenji Maekawa, Hajime Minakuchi, Hirofumi Yatani, Glenn T Clark, Yoshizo Matsuka, Takuo Kuboki

    Archives of oral biology   52 ( 8 )   797 - 802   2007年8月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    AIM: This study focused on the relationship between the HPA axis function and the heat pain threshold in the orofacial region upon cold pressor (CP) stimulation. METHODS: Ten healthy male individuals participated in this study. CP stimulation was applied to each participant, and their peripheral blood was collected 5 min before, during and 5, 15, 30, 45, 60 min after receiving CP. In addition, 5 of those 10 participants were selected at random and they experienced a mock CP trial on different days. The heat pain thresholds on the facial skin about 10mm anterior to the right external auditory canal (trigeminal V2 region) in each subject were simultaneously recorded 5 min before and 5, 30, 60 min after CP stimulation. The blood pressure and heart rate were continuously monitored throughout the course of the CP and mock trials using an electric blood pressure meter. RESULTS: Significant increases in the plasma concentration of cortisol, beta-endorphin and ACTH were induced by CP stimulation, while no significant increases were observed under the mock trial conditions. The blood pressure and heart rate showed concomitant increases during CP stimulation. In addition, the heat pain threshold in the orofacial region significantly increased after receiving CP stimulation. These results suggest that CP stimulation activated the HPA axis thereby increasing the heat pain threshold in the orofacial region in healthy individuals. CONCLUSIONS: This observed pain threshold increase might be due to the activation of an endogenous opioid system, such as increase in the circulating beta-endorphin levels.

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  • Responses of the hypothalamic-pituitary-adrenal axis and pain threshold changes in the orofacial region upon cold pressor stimulation in normal volunteers

    Koji Suzuki, Kenji Maekawa, Hajime Minakuchi, Hirofumi Yatani, Glenn T. Clark, Yoshizo Matsuka, Takuo Kuboki

    ARCHIVES OF ORAL BIOLOGY   52 ( 8 )   797 - 802   2007年8月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Aim: This study focused on the relationship between the HPA axis function and the heat pain threshold in the orofacial region upon cold pressor (CP) stimulation.
    Methods: Ten healthy male individuals participated in this study. CP stimulation was applied to each participant, and their peripheral blood was collected 5 min before, during and 5, 15, 30, 45, 60 min after receiving CP. In addition, 5 of those 10 participants were selected at random and they experienced a mock CP trial on different days. The heat pain thresholds on the facial skin about 10 mm anterior to the right external auditory canal (trigeminal V2 region) in each subject were simultaneously recorded 5 min before and 5, 30, 60 min after CP stimulation. The blood pressure and heart rate were continuously monitored throughout the course of the CP and mock trials using an electric blood pressure meter.
    Results: Significant increases in the plasma concentration of cortisol, P-endorphin and ACTH were induced by CP stimulation, while no significant increases were observed under the mock trial conditions. The blood pressure and heart rate showed concomitant increases during CP stimulation. In addition, the heat pain threshold in the orofacial region significantly increased after receiving CP stimulation. These results suggest that CP stimulation activated the HPA axis thereby increasing the heat pain threshold in the orofacial region in healthy individuals.
    Conclusions: This observed pain threshold increase might be due to the activation of an endogenous opioid system, such as increase in the circulating P-endorphin levels. (C) 2007 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.archoralbio.2007.01.008

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  • Chemical interaction of polyphosphoric acid with titanium and its effect on human bone marrow derived mesenchymal stem cell behavior

    Kenji Maekawa, Yasuhiro Yoshida, Atsushi Mine, Takuo Fujisawa, Bart Van Meerbeek, Kazuomi Suzuki, Takuo Kuboki

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   82A ( 1 )   195 - 200   2007年7月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    The aim of this study was to evaluate the effect of treating titanium (Ti) with polyphosphoric acid on the attachment and proliferation of human bone marrow derived mesenchymal stem cells (hBMSCs). Cleaned Ti disks were immersed into three different concentrations of polyphosphoric acid solution (0.1, 1, and 10 wt %) and 10 wt % orthophosphoric acid solution for 24 h at 37 C. Ti immersed in distilled water for 24 h at 37 C served as control. The level of polyphosphoric acid that interacted with the Ti surface was determined by measuring the surface P/Ti ratio (atom%/atom%) using X-ray photoelectron spectroscopy. Degrees of cell attachment (1, 3, 5 h after cell seed) and proliferation (l, 3, 5, and 7 days after cell seed) on each treated Ti disk were evaluated by MTS assay. The mean surface P/Ti ratios increased in a polyphosphoric acid concentration dependent manner. A significantly higher cell attachment was found on Ti treated with polyphosphoric acid in contrast to untreated Ti (control) for all three culture periods. MTS assay also revealed that cell proliferation levels significantly increased following a polyphosphoric acid dose dependency. Ti surface treatment with orthophosphoric acid did not influence the cell attachment and proliferation. It was concluded that polyphosphoric acid treatment of Ti enhanced the attachment and proliferation of hBMSCs. (c) 2007 Wiley Periodicals, Inc.

    DOI: 10.1002/jbm.a.31139

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  • Chemical interaction of polyphosphoric acid with titanium and its effect on human bone marrow derived mesenchymal stem cell behavior. 国際誌

    Kenji Maekawa, Yasuhiro Yoshida, Atsushi Mine, Takuo Fujisawa, Bart Van Meerbeek, Kazuomi Suzuki, Takuo Kuboki

    Journal of biomedical materials research. Part A   82 ( 1 )   195 - 200   2007年7月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    The aim of this study was to evaluate the effect of treating titanium (Ti) with polyphosphoric acid on the attachment and proliferation of human bone marrow derived mesenchymal stem cells (hBMSCs). Cleaned Ti disks were immersed into three different concentrations of polyphosphoric acid solution (0.1, 1, and 10 wt %) and 10 wt % orthophosphoric acid solution for 24 h at 37 degrees C. Ti immersed in distilled water for 24 h at 37 degrees C served as control. The level of polyphosphoric acid that interacted with the Ti surface was determined by measuring the surface P/Ti ratio (atom%/atom%) using X-ray photoelectron spectroscopy. Degrees of cell attachment (1, 3, 5 h after cell seed) and proliferation (1, 3, 5, and 7 days after cell seed) on each treated Ti disk were evaluated by MTS assay. The mean surface P/Ti ratios increased in a polyphosphoric acid concentration dependent manner. A significantly higher cell attachment was found on Ti treated with polyphosphoric acid in contrast to untreated Ti (control) for all three culture periods. MTS assay also revealed that cell proliferation levels significantly increased following a polyphosphoric acid dose dependency. Ti surface treatment with orthophosphoric acid did not influence the cell attachment and proliferation. It was concluded that polyphosphoric acid treatment of Ti enhanced the attachment and proliferation of hBMSCs.

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  • 金属アレルギー患者に対するインプラント治療 チタン製修復物による暴露試験を行った1症例

    完山 学, 真野 隆充, 荒川 光, 峯 篤史, 上山 吉哉, 窪木 拓男

    日本口腔インプラント学会誌   20 ( 2 )   293 - 298   2007年6月

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    記述言語:日本語   出版者・発行元:(公社)日本口腔インプラント学会  

    70歳女。金銀パラジウム合金を用いたレジン前装冠ブリッジを装着したが、上唇のヒリヒリ感、ブヨブヨ感を訴え、パノラマX線で残存歯に僅かな水平的な骨吸収が認めれた。上顎前歯部ブリッジに対する金属アレルギーが疑いパッチテストを施行し陽性であれば、金属製の歯冠修復物を除去し、ハイブリッドセラミックなどの材料に置換すると説明し了解を得た。パッチテストの結果、塩化コバルト、塩化パラジウムに陽性反応を認めた。上顎前歯部ブリッジ金属(パラジウム)に対するアレルギーと診断し、口腔内に存在する全ての金銀パラジウム修復物、補綴物の除去を行い、上顎前歯部ブリッジに対しては即時重合レジンを用いた暫間ブリッジを装着し、それ以外の除去部位にはハイブリッドセラミックによるジャケット冠やインレーを装着した。ハイブリッドセラミックで製作を予定したが歯根が破折したためチタン製インプラント補綴修復を提案し長期曝露試験を行いアレルギーがなく使用可能となった。

    DOI: 10.11237/jsoi.20.293

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  • Promotion of attachment of human bone marrow stromal cells by CCN2. 国際誌

    Mitsuaki Ono, Satoshi Kubota, Takuo Fujisawa, Wataru Sonoyama, Harumi Kawaki, Kentaro Akiyama, Masamitsu Oshima, Takashi Nishida, Yasuhiro Yoshida, Kazuomi Suzuki, Masaharu Takigawa, Takuo Kuboki

    Biochemical and biophysical research communications   357 ( 1 )   20 - 5   2007年5月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Cell attachment is a crucial step in tissue regeneration. In this study, human bone marrow stromal cells (hBMSCs) were isolated, and the effects of CCN2 on their attachment were examined. CCN2 significantly enhanced the hBMSC attachment, and this enhanced cell attachment was mainly regulated by the C-terminal module of CCN2. This enhancement was negated by the anti-integrin alpha(v)beta(3) antibody and p38 MAPK inhibitor, and phosphorylation of p38 MAPK was detected upon the enhanced cell attachment mediated by CCN2. We thus conclude that CCN2 enhances hBMSC attachment via integrin-p38 MAPK signal pathway. Enhanced hBMSC attachment on hydroxyapatite plates by CCN2 further indicated the utility of CCN2 in bone regeneration.

    DOI: 10.1016/j.bbrc.2007.03.052

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  • Muscle contraction accelerates IL-6 mRNA expression in the rat masseter muscle

    Tsuyoshi Ono, Kenji Maekawa, Shogo Watanabe, Hisao Oka, Takuo Kuboki

    ARCHIVES OF ORAL BIOLOGY   52 ( 5 )   479 - 486   2007年5月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: : This study was conducted to determine if interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) mRNA expression increase in response to muscle contraction caused by repetitive electrical stimulation of the rat masseter muscle.
    Methods: : Male Wistar rats weighing 140-160 g were divided randomly into the following three groups: electrical stimulation (ES) group (n = 21), carrageenan injection (CI) group (n = 24), and ES under dantrolene sodium (muscle relaxant) injection (ESDI) group (n = 7). ES or CI was done to the left masseter; and mock ES or mock Cl to the right. Muscle tissues on both sides were sampled for total RNA isolation. Real-time RT-PCR was performed, with the cyclophilin A (CypA) mRNA level in each sample as an internal control. Mean relative IL-6 (il-6/cypA) and IL-1 beta (il-1 beta/cypA) mRNA levels were compared between the experimental and mock-treated sides within each group.
    Results: Mean IL-6/CypA levels in the ES- or Cl-treated muscle significantly increased, without any significant incremental change observed in either mock-treated muscle. Interestingly, the increase in the il-6/cypA level caused by the ES was suppressed by the injection of dantrolene sodium in the ESDI group. Furthermore, the mean il-1 beta/cypA level in the Cl-treated masseter also significantly increased without any significant incremental change observed in the mock-treated muscle. However, there was no significant difference in the mean il-1 beta/cypA levels in the masseter between the ES- and the mock-treated sides.
    Conclusions: : These results show that IL-6 mRNA expression in the rat masseter muscle was accelerated by the CI or by repetitive muscle contraction induced by ES. Since the mRNA level of IL-1 beta, a well-known proinflammatory cytokine, was not altered by the contraction, the accelerated IL-6 mRNA expression elicited by the muscle contraction does not seem to be related to local inflammation. (c) 2006 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.archoralbio.2006.10.025

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  • Promotion of attachment of human bone marrow stromal cells by CCN2

    Mitsuaki Ono, Satoshi Kubota, Takuo Fujisawa, Wataru Sonoyama, Harumi Kawaki, Kentaro Akiyama, Masamitsu Oshima, Takashi Nishida, Yasuhlro Yoshida, Kazuomi Suzuki, Masaharu Takigawa, Takuo Kuboki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   357 ( 1 )   20 - 25   2007年5月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Cell attachment is a crucial step in tissue regeneration. In this study, human bone marrow stromal cells (hBMSCs) were isolated, and the effects of CCN2 on their attachment were examined. CCN2 significantly enhanced the hBMSC attachment, and this enhanced cell attachment was mainly regulated by the C-terminal module of CCN2. This enhancement was negated by the anti-integrin alpha(v)beta(3) antibody and p38 MAPK inhibitor, and phosphorylation of p38 MAPK was detected upon the enhanced cell attachment mediated by CCN2. We thus conclude that CCN2 enhances hBMSC attachment via integrin-p38 MAPK signal pathway. Enhanced hBMSC attachment on hydroxyapatite plates by CCN2 further indicated the utility of CCN2 in bone regeneration. (c) 2007 Elsevier Inc. All rights reserved.

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  • Muscle contraction accelerates IL-6 mRNA expression in the rat masseter muscle. 国際誌

    Tsuyoshi Ono, Kenji Maekawa, Shogo Watanabe, Hisao Oka, Takuo Kuboki

    Archives of oral biology   52 ( 5 )   479 - 86   2007年5月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    OBJECTIVE: This study was conducted to determine if interleukin-6 (IL-6) and interleukin-1beta (IL-1beta) mRNA expression increase in response to muscle contraction caused by repetitive electrical stimulation of the rat masseter muscle. METHODS: Male Wistar rats weighing 140-160 g were divided randomly into the following three groups: electrical stimulation (ES) group (n=21), carrageenan injection (CI) group (n=24), and ES under dantrolene sodium (muscle relaxant) injection (ESDI) group (n=7). ES or CI was done to the left masseter; and mock ES or mock CI to the right. Muscle tissues on both sides were sampled for total RNA isolation. Real-time RT-PCR was performed, with the cyclophilin A (CypA) mRNA level in each sample as an internal control. Mean relative IL-6 (il-6/cypA) and IL-1beta (il-1beta/cypA) mRNA levels were compared between the experimental and mock-treated sides within each group. RESULTS: Mean IL-6/CypA levels in the ES- or CI-treated muscle significantly increased, without any significant incremental change observed in either mock-treated muscle. Interestingly, the increase in the il-6/cypA level caused by the ES was suppressed by the injection of dantrolene sodium in the ESDI group. Furthermore, the mean il-1beta/cypA level in the CI-treated masseter also significantly increased without any significant incremental change observed in the mock-treated muscle. However, there was no significant difference in the mean il-1beta/cypA levels in the masseter between the ES- and the mock-treated sides. CONCLUSIONS: These results show that IL-6 mRNA expression in the rat masseter muscle was accelerated by the CI or by repetitive muscle contraction induced by ES. Since the mRNA level of IL-1beta, a well-known proinflammatory cytokine, was not altered by the contraction, the accelerated IL-6 mRNA expression elicited by the muscle contraction does not seem to be related to local inflammation.

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  • Comparison of inter-twin concordance in symptoms of temporomandibular disorders: a preliminary investigation in an adolescent twin population. 国際誌

    Yoshizo Matsuka, Chiyomi Nagamatsu, Shingo Itoh, Takayuki Tomonari, Ali Makki, Hajime Minakuchi, Kenji Maekawa, Manabu Kanyama, Takuo Kuboki

    Cranio : the journal of craniomandibular practice   25 ( 1 )   23 - 9   2007年1月

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    記述言語:英語   出版者・発行元:CHROMA INC  

    There is controversy as to the genetic contribution to the pathogenesis of temporo-mandibular disorders (TMD). Several reports reveal a marked familial aggregation in the signs and symptoms of TMD, while others do not. Therefore, our goal was to investigate the hypothesis using a sophisticated research design, which was a well-known genetic survey inter-twin concordance assessment in the symptoms of TMD. This study is the first step to survey TMD symptoms of a twin group in junior and high schools as a preliminary trial. The 63 twins were asked to participate in this study in junior and senior high schools affiliated with the University of Tokyo, Japan, schools which kept ten twins in each grade for the purpose of several genetic survey programs. After excluding incomplete filling out of questionnaire sheets and data from male-female pairs, 43 monozygotic (MZ) (15.3+/-1.7 yrs, male/female = 17/26 pairs) and nine dyzygotic (DZ) (15.2+/-1.8 yrs, male/female = 6/3 pairs) twins were studied. Outcomes consisted of a prevalidated 14-item self-administered questionnaire, which assessed proband- and pair-wise concordance levels in the MZ and DZ twins. These results demonstrated that the MZ twins had a higher tendency of inter-twin concordance than DZ twins in terms of jaw pain in wide mouth opening (proband-wise concordance = 66.7% in MZ, 0% in DZ), difficulty in mouth opening (20% in MZ, 0% in DZ) and difficulty in mouth closing (50.0% in MZ, 33.3% in DZ), while there was no significant difference between the MZ and DZ concordance levels in other general health-related and behavior-related items, except toothache. However, the pair-wise concordance rates of jaw pain in wide mouth opening and difficulty in mouth opening in the MZ twins were not significantly higher compared to the DZ rate. Possibly, a genetic factor contributed to the pathogenesis of TMD in an adolescent population. The sample size needs to be increased, and there are plans to survey the next sample in the same schools.

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  • Comparison of inter-twin concordance in symptoms of temporomandibular disorders: A preliminary investigation in an adolescent twin population

    Yoshizo Matsuka, Chiyomi Nagamatsu, Shingo Itoh, Takayuki Tomonari, Ali Makki, Hajime Minakuchi, Kenji Mackawa, Manabu Kanyama, Takuo Kuboki

    CRANIO-THE JOURNAL OF CRANIOMANDIBULAR PRACTICE   25 ( 1 )   23 - 29   2007年1月

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    記述言語:英語   出版者・発行元:CHROMA INC  

    There is controversy as to the genetic contribution to the pathogenesis of temporomandibular disorders (TMD). Several reports reveal a marked familial aggregation in the signs and symptoms of TMD, while others do not. Therefore, our goal was to investigate the hypothesis using a sophisticated research design, which was a well-known genetic survey inter-twin concordance assessment in the symptoms of TMD. This study is the first step to survey TMD symptoms of a twin group in junior and high schools as a preliminary trial. The 63 twins were asked to participate in this study in junior and senior high schools affiliated with the University of Tokyo, Japan, schools which kept ten twins in each grade for the purpose of several genetic survey programs. After excluding incomplete filling out of questionnaire sheets and data from male-female pairs, 43 monozygotic (MZ) (15.3 +/- 1.7 yrs, male/female = 17/26 pairs) and nine dyzygotic (DZ) (15.2 +/- 1.8 yrs, male/female = 6/3 pairs) twins were studied. Outcomes consisted of a prevaliclated 14-item self-administered questionnaire, which assessed proband- and pair-wise concordance levels in the MZ and DZ twins. These results demonstrated that the MZ twins had a higher tendency of inter-twin concordance than DZ twins in terms of jaw pain in wide mouth opening (proband-wise concordance = 66.7% in MZ, 0% in DZ), difficulty in mouth opening (20% in MZ, 0% in DZ) and difficulty in mouth closing (50.0% in MZ, 33.3% in DZ), while there was no significant difference between the MZ and DZ concordance levels in other general health-related and behavior-related items, except toothache. However, the pair-wise concordance rates of jawpain in wide mouth opening and difficulty in mouth opening in the MZ twins were not significantly higher compared to the DZ rate. Possibly, a genetic factor contributed to the pathogenesis of TMD in an adolescent population. The sample size needs to be increased, and there are plans to survey the next sample in the same schools.

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  • Cell proliferation on Titania layer with in vitro apatite forming ability

    S. Yabe, K. Tsuru, S. Hayakawa, A. Osaka, Y. Yoshida, K. Suzuki, T. Kuboki

    BIOCERAMICS, VOL 19, PTS 1 AND 2   330-332   131 - +   2007年

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    記述言語:英語   出版者・発行元:TRANS TECH PUBLICATIONS LTD  

    Titania layer was fabricated on the titanium substrates with chemical treatment with 20ml or 40ml of hydrogen peroxide solution and subsequent heat treatment at 400 degrees C, coded as CHT20 and CHT40, respectively. CHT20 spontaneously deposited apatite on the surface in a simulated body fluid (SBF), while CHT40 did not. TF-XRD patterns showed that the diffraction intensity of anatase of CHT20 was higher than that of CHT40. It was suggested that the thicker titania layer indicated in vitro apatite forming ability. The cell proliferation of CHT20 and CHT40 were lower than NT and HT. Since the surface of titania layers became hydrophobic after autoclaving, we can suppose that the cell proliferation on CHT20 and CHT40 were lower than NT and HT due to their surface hydrophobicity.

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  • 附属病院の歯科系広報誌から,附属病院,研究科,学部をまとめた岡山大学歯学部総合情報交換誌「歯学だより」へ

    窪木拓男

    歯学だより   3   7   2007年

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  • 今年の夏は岡山歯学会に集合しよう

    窪木拓男

    岡山大学歯学部広報   2007年

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  • Cell proliferation on Titania layer with in vitro apatite forming ability

    S. Yabe, K. Tsuru, S. Hayakawa, A. Osaka, Y. Yoshida, K. Suzuki, T. Kuboki

    BIOCERAMICS, VOL 19, PTS 1 AND 2   330-332   131 - +   2007年

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    記述言語:英語   出版者・発行元:TRANS TECH PUBLICATIONS LTD  

    Titania layer was fabricated on the titanium substrates with chemical treatment with 20ml or 40ml of hydrogen peroxide solution and subsequent heat treatment at 400 degrees C, coded as CHT20 and CHT40, respectively. CHT20 spontaneously deposited apatite on the surface in a simulated body fluid (SBF), while CHT40 did not. TF-XRD patterns showed that the diffraction intensity of anatase of CHT20 was higher than that of CHT40. It was suggested that the thicker titania layer indicated in vitro apatite forming ability. The cell proliferation of CHT20 and CHT40 were lower than NT and HT. Since the surface of titania layers became hydrophobic after autoclaving, we can suppose that the cell proliferation on CHT20 and CHT40 were lower than NT and HT due to their surface hydrophobicity.

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  • 附属病院の歯科系広報誌から,附属病院,研究科,学部をまとめた岡山大学歯学部総合情報交換誌「歯学だより」へ

    窪木拓男

    歯学だより   3   7   2007年

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  • 今年の夏は岡山歯学会に集合しよう

    窪木拓男

    岡山大学歯学部広報   2007年

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  • Current research directions and management innovations for temporomandibular disorders and orofacial pain.

    窪木拓男

    The Twelfth Scientific Meeting Asian Academy of Craniomandibular Disorders.Program and Abstracts,   21   2007年

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  • 目指せ,日本一の医学部・歯学部附属病院

    窪木拓男

    Okayama University Hospital Monthly Letter   2007年

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  • 第20回日本顎頭蓋機能学会記念学術大会を終えて

    窪木拓男

    日本顎頭蓋機能学会誌   19 ( 1 )   巻頭言   2007年

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  • 金属アレルギー患者に対するインプラント治療ーチタン製修復物による暴露試験を行った一症例ー

    完山 学, 真野隆充, 荒川 光, 峯 篤史, 上山吉哉, 窪木拓男

    日本口腔インプラント学会雑誌   20 ( 2 )   293 - 298   2007年

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  • 岡山大学歯学部5年次生に対する口腔インプラント実習の試み

    完山 学, 荒川 光, 園山 亘, 北村洋一, 木村 彩, 野田欣志, 川上 彩, 松香芳三, 窪木拓男

    岡山歯学会雑誌   26 ( 2 )   119 - 124   2007年

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  • 目指せ,日本一の医学部・歯学部附属病院

    窪木拓男

    Okayama University Hospital Monthly Letter   2007年

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  • 歯科補綴研究に役立つ統計学-臨床研究における予測・結果因子,統計解析-

    窪木拓男, 水口 一, 荒川 光

    日本補綴歯科学会雑誌   51 ( 1 )   1   2007年

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  • 研究プロジェクトの精鋭たち:患者のQOLの維持と向上に貢献できる歯科医療をめざして「岡山大学大学院医歯薬学総合研究科顎口腔機能制御学分野(旧歯科補綴学第一講座)」

    窪木拓男

    ザ・クインテッセンス   26 ( 8 )   144 - 144   2007年

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  • 歯科治療における再生医療のニーズと現時点での研究動向-確実かつ質の高いインプラント治療をめざして-

    窪木拓男

    岡山歯学会雑誌   26 ( 1 )   1 - 14   2007年

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  • 歯科補綴研究に役立つ統計学-臨床研究における予測・結果因子,統計解析-

    窪木拓男, 水口 一, 荒川 光

    日本補綴歯科学会雑誌   51 ( 1 )   1   2007年

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  • Responses of the Hypothalamic-Pituitary-Adrenal axis and pain threshold changes in the orofacial region upon cold pressor stimulation in normal volunteers.

    Suzuki K, Maekawa K, Minakuchi H, Yatani H, Clark G T, Matsuka Y, Kuboki T

    Archives of oral biology   52   797 - 802   2007年

  • Responses of the hypothalamic-pituitary-adrenal axis and pain threshold changes in the orofacial region upon cold pressor stimulation in normal volunteers

    Suzuki K, Maekawa K, Minakuchi H, Yatani H, Clark GT, Matsuka Y, Kuboki T

    Archives of Oral Biology   52 ( 8 )   797 - 802   2007年

  • 歯科治療における再生医療のニーズと現時点での研究動向-確実かつ質の高いインプラント治療をめざして-

    窪木拓男

    岡山歯学会雑誌   26 ( 1 )   1 - 14   2007年

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  • 第20回日本顎頭蓋機能学会記念学術大会を終えて

    窪木拓男

    日本顎頭蓋機能学会誌   19 ( 1 )   巻頭言   2007年

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  • 岡山大学歯学部5年次生に対する口腔インプラント実習の試み

    完山 学, 荒川 光, 園山 亘, 北村洋一, 木村 彩, 野田欣志, 川上 彩, 松香芳三, 窪木拓男

    岡山歯学会雑誌   26 ( 2 )   119 - 124   2007年

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  • 生体工学を応用した三叉神経痛治療のための遺伝子導入法の開発

    松香芳三, 北村洋一, 福田朋樹, 完山 学, 前川賢治, 窪木拓男

    日本歯科医学会雑誌   26 ( 133 )   2007年

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  • 生体工学を応用した三叉神経痛治療のための遺伝子導入法の開発

    松香芳三, 北村洋一, 福田朋樹, 完山 学, 前川賢治, 窪木拓男

    日本歯科医学会雑誌   26 ( 133 )   2007年

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  • Regression of pustulosis palmaris et plantaris by periodontal treatment in a subject with severe periodontitis. 国際誌

    Hiroshi Akazawa, Fushanori Nishimura, Hiroshi Maeda, Shogo Takashiba, Atsushi Mine, Kenji Maekawa, Takuo Kuboki

    International journal of dermatology   45 ( 12 )   1420 - 2   2006年12月

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING  

    A 38-year-old woman with pustulosis palmaris et plantaris (PPP) was referred to the dentist with a suspicion of metal allergy (Fig. 1A). She had noticed the symptoms 3 years previously on her palms and legs. As her symptoms did not improve spontaneously, she visited her dermatologist. There, she was recorded as follows: (1) pustules localized only on the palms and soles; (2) no lesions with eczema or psoriasis on any other parts of the body; and (3) no family history of pustulosis. On the basis of these observations, she was diagnosed with PPP. She was treated with topical application of corticosteroids; however, her symptoms did not improve. Her dermatologist then suspected metal allergy and performed a patch test. Her skin reacted positively with chromium sulfate, zinc chloride, and mercury bichloride. Therefore, she was referred to our dental hospital for the investigation of the possible association of PPP with oral restorative materials.
    Her medical records were unremarkable, except for extremely severe periodontitis, which she was aware of more than 10 years previously; however, she had not received any comprehensive periodontal treatment. On presentation, we first analyzed the restorative materials of the teeth to determine whether they contained any positive elements detected by the patch test. Unfortunately, we could not detect any positive elements in the oral cavity. Her blood chemistry did not indicate any abnormalities. The white blood cell count was 5600/mm(3), with differential counts of 49.3% neutrophils, 28.0% lymphocytes, 7.6% monocytes, 14.4% eosinophils, and 0.7% basophils. C-reactive protein was not elevated, as measured by the conventional method. Immunoglobulin G (IgG), IgA, and IgM concentrations were all within the normal range (IgG, 1243.0 mg/dL; IgA, 131.6 mg/dL; IgM, 149.6 mg/dL). Because of these laboratory data, we first treated her periodontitis to determine whether there was a possible relationship of her PPP to severe periodontitis.
    Her periodontitis was very severe (Fig. 2A,B). The extraction of several teeth, as well as the correction of the shape of the inflamed alveolar bone (the bone supporting the teeth) to a physiological shape, was necessary. We first treated her periodontitis with the topical application of antibiotics (minocycline-HCl ointment) to determine the effect of anti-infectious periodontal treatment on PPP. We administered the antibiotics in every periodontal pocket once a week for a period of 1 month. One month after treatment, her symptoms appeared to improve slightly (Fig. 1B). We next performed surgical treatment, including teeth extractions and correction of the bone shape. Two to three days after surgery, her PPP symptoms worsened suddenly (Fig. 1C), and continued for up to 1 month (Fig. 1D). From 1 month after surgery, however, her symptoms gradually improved and complete remission was observed (Fig. 1E). In addition, although more than 2 years have passed since complete remission, no recurrence has been observed (Fig. 1F).

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  • Spectroscopic characterization of enamel surfaces irradiated with Er:YAG laser.

    Atsushi Mine, Yasuhiro Yoshida, Kazuomi Suzuki, Youichi Nakayama, Hirofumi Yatani, Takuo Kuboki

    Dental materials journal   25 ( 2 )   214 - 8   2006年6月

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    記述言語:英語   出版者・発行元:JAPANESE SOC DENTAL MATERIALS DEVICES  

    The purpose of this study was to investigate the effect of laser irradiation on the enamel surface spectroscopically. Polished bovine enamel surface was irradiated with an Er:YAG laser apparatus and chemically analyzed using X-ray photoelectron spectroscopy (XPS). XPS detected core electron peaks such as carbon (C 1s) and nitrogen (N 1s), as well as calcium (Ca 2s, Ca 2p, Ca 3s, Ca 3p), phosphorous (P 2s, P 2p), and oxygen (O 1s), from the control enamel surface. After irradiation, XPS revealed that the intensity of C 1s peak slightly decreased while that of N 1s diminished below the detection limit. Detailed C 1s narrow scan spectrum of the Er:YAG laser-irradiated enamel surface showed that C-O/C-N, C = O/-CON=, -COO-, and CO,3(2-) components, attributed to the specific composition of enamel and any common carbon contamination in surplus, relatively decreased. The binding energies of Ca 2p and P 2p, delta (Ca 2p, P 2p), and the Ca/P ratio of lased enamel were found to be different from those of non-lased enamel. It was also shown that Er:YAG laser ablation caused surface alterations to the apatitic inorganic components of tooth tissue.

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  • 顎関節MRIを用いた関節円板動態診断の変動と信頼性

    永松千代美, 小野剛, 前川賢治, 柳文修, 浅海淳一, 荒木佳子, 片山朗, 谷本裕子, 宮脇正一, 山本照子, 岸幹二, 窪木拓男

    日本顎関節学会雑誌   18 ( 1 )   60 - 60   2006年4月

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    記述言語:日本語   出版者・発行元:(一社)日本顎関節学会  

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  • ASIC3-immunoreactive neurons in the rat vagal and glossopharyngeal sensory ganglia

    T Fukuda, H Ichikawa, R Terayama, T Yamaai, T Kuboki, T Sugimoto

    BRAIN RESEARCH   1081   150 - 155   2006年4月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    ASIC3-immunoreactivity (ir) was examined in the rat vagal and glossopharyngeal sensory ganglia. In the jugular, petrosal and nodose ganglia, 24.8%, 30.8% and 20.6% of sensory neurons, respectively, were immunoreactive for ASIC3. These neurons were observed throughout the ganglia. A double immuno fluorescence method demonstrated that many ASIC3-immunoreactive (ir) neurons co-expressed calcitonin gene-related peptide (CGRP)- or vanilloid receptor subtype 1 (VRL-1)-ir in the jugular (CGRP, 77.8%; VRL-1, 28.0%) and petrosal ganglia (CGRP, 61.7%; VRL-1, 21.5%). In the nodose ganglion, however, such neurons were relatively rare (CGRP, 6.3%; VRL-1, 0.4%). ASIC3-ir neurons were mostly devoid of tyrosine hydroxylase in these ganglia. However, some ASIC3-ir neurons co-expressed calbindin D28k in the petrosal (5.5%) and nodose ganglia (3.8%). These findings may suggest that ASIC3-containing neurons have a wide variety of sensory modalities in the vagal and glossopharyngeal sensory ganglia. (c) 2006 Elsevier B.V. All rights reserved.

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  • Effect of 4-MET- and 10-MDP-based primers on resin bonding to titanium.

    Youhei Tsuchimoto, Yasuhiro Yoshida, Atsushi Mine, Mariko Nakamura, Norihiro Nishiyama, Bart Van Meerbeek, Kazuomi Suzuki, Takuo Kuboki

    Dental materials journal   25 ( 1 )   120 - 4   2006年3月

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    記述言語:英語   出版者・発行元:JAPANESE SOC DENTAL MATERIALS DEVICES  

    The purpose of this study was to investigate the effect of a 4-MET- and 10-MDP-based primer on the bond strength of two resin cements (SuperBond C&B, Sun Medical; Panavia Fluoro Cement, Kuraray) to titanium (Ti). Ti plates were treated with six experimental primers consisting of, respectively, 10-MDP and 4-MET in concentrations of 0.1, 1 and 10wt%, or were kept untreated (control). The highest tensile bond strength of Panavia Fluoro Cement to Ti was obtained when the Ti surface was pre-treated with 10wt% 10-MDP and was significantly higher than that when a lower concentrated 10-MDP-based primer or any 4-MET-based primer was used. On the contrary, no significant difference in tensile bond strength of SuperBond C&B was found for the untreated and six pre-treated Ti surfaces, although pre-treatment with each 10-MDP-based primer resulted in a higher tensile bond strength as compared to any 4-MET pre-treatment. Altogether, the data obtained strongly suggest that 10-MDP is effective to improve the adhesive performance of resin to titanium.

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  • 特集 Orofacial Pain-歯科はどこまでかかわるか- 第3部 精神疾患と歯科治療-特に咬合感覚異常症の疾患概念確立に向けて-

    窪木拓男

    歯界展望   108(5):1019-1025   2006年

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  • ヘパリン結合活性を増強した遺伝子改変BMP-2を徐放するキャリアの最適化とその効果 査読

    下野賢吾, 大野充昭, 大島正充, 木村祐, Sebald W, 洲鎌和茂, 田畑泰彦, 窪木拓男

    第9回日本組織工学会(2006.9.7-8 京都)   2006年

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  • 萎縮上顎無歯顎へのZygoma implant fixture systemの手術経験.

    植野高章, 坂田吉朗, 香川智正, 加納みわ, 本多浩三, 脇本真理, 水川展吉, 福永城司, 窪木拓男, 菅原利夫

    岡山歯学会雑誌   2006年

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  • CCN ファミリータンパク質の内軟骨性骨化制御機構と骨・軟骨再生作用 査読

    久保田聡, 椋代義樹, 菊池剛, 大野充昭, 川木晴美, 柳田剛志, 西田崇, 田畑泰彦, 窪木拓男, 滝川正春

    第24 回日本骨代謝学会シンポジウム 硬組織再生研究の最前線(2006.7.7. 東京)   2006年

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    記述言語:日本語  

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  • 日本人を対象とした口腔インプラント治療の臨床成績に関するSystematic Review.

    荒川 光, 窪木拓男

    歯界展望   108,1,131-144   2006年

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  • 歯質欠損,部分歯列欠損,無歯顎に対する症型分類の提案.

    市川哲雄, 佐藤博信, 窪木拓男, 佐藤裕二, 秀島雅之, 和気裕之, 安田 登, 服部正巳, 貞森紳丞, 尾関雅彦, 友竹偉則, 長尾 寛, 大山喬史, 赤川安正

    日本歯科医学会誌   25,63-75   2006年

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  • 特集 Orofacial Pain-歯科はどこまでかかわるか- 企画にあたって なぜ今, 歯科医が口腔顔面痛や口腔運動器疾患, 精神医学的問題を学ばなければならないのか?

    窪木拓男, 前川賢治

    歯界展望   108,4,684   2006年

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  • 歯ぎしりセンサーの評価と使い方.

    水口 一, 窪木拓男

    補綴臨床   39,5,536-541   2006年

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  • 貼付型ブラキシズム診断装置「BiteStrip」について.

    水口 一, 窪木拓男

    日本歯科評論   66,6,113-116   2006年

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  • Immunohistochemical localization of gamma and beta subunits of epithelial Na+ channel in the rat molair tooth pulp

    H Ichikawa, T Fukuda, R Terayama, T Yamaai, T Kuboki, T Sugimoto

    BRAIN RESEARCH   1065 ( 1-2 )   138 - 141   2005年12月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    The distribution of gamma and beta subunits of epithelial Na+ channel (ENaC), markers for low-threshold mechanoreceptors in peripheral tissues, was examined in the tooth pulp. In the root pulp, gamma ENaC- and beta ENaC-immunoreactive (IR) nerve fibers showed a thick smooth appearance. These nerve fibers ascended toward the pulp horn and formed subodontoblastic nerve plexuses. Immunoelectron microscopic method revealed that 63% of axons were immunoreactive for gamma ENaC in the root pulp. Virtually all myelinated axons showed gamma ENaC-IR (97%), whereas unmyelinated axons were mostly devoid of it (12%). These findings suggest that myelinated tooth pulp nociceptors; respond to mechanical stimuli. (C) 2005 Elsevier B.V. All rights reserved.

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  • Stress induces changes of internal standard genes of amygdala. 国際誌

    Shigeru Maeda, Ichiro Nakatsuka, Takuya Miyawaki, Takuo Kuboki, Masahiko Shimada

    Brain research. Molecular brain research   140 ( 1-2 )   133 - 7   2005年10月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    In this study, we tested changes of internal standard genes of the brain after electrical tail shock in rats. The level of 28S rRNA of the amygdala was increased significantly after the stress. There was no significant change in GAPDH, beta-actin, 36B4 mRNA and 28S rRNA at the subfornical organ and the hippocampus.

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  • 服薬状況を用いて評価した持続性神経障害性疼痛患者の長期治療経過

    永松 千代美, 縄稚 久美子, 峯 篤史, 前川 賢治, 窪木 拓男

    日本補綴歯科学会雑誌   49 ( 5 )   691 - 696   2005年10月

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    記述言語:日本語   出版者・発行元:(公社)日本補綴歯科学会  

    56歳女.上顎左側第一大臼歯相当歯槽部の慢性灼熱痛で受診した.慢性辺縁性歯周炎の診断で無麻酔下で歯肉縁下の盲嚢掻爬を受けた直後より同部の持続性慢性灼熱痛を訴えるようになり,疼痛部位である第二小臼歯と第一大臼歯の抜髄処置を受けた.痛みが軽減しないため,難治性の根尖性歯周炎の診断のもと,第二小臼歯の抜歯を受けた.抜歯後,痛みが激烈となった.持続性神経障害性疼痛(非定型性歯痛)の臨床診断基準にほぼ当てはまり,左側上顎臼歯部の無麻酔下盲嚢掻爬に端を発した持続性神経障害性疼痛(非定型性歯痛)と診断した.カプサイシン局所塗布,三環系抗うつ剤を中心とした薬物療法,簡易精神療法を行い痛みの程度に減少傾向を認めた

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  • Connective tissue growth factor (CTGF/CCN2) reinforces the molecular phenotype of aauricular chondrocytes in vitro.

    T Fujisawa, K Nakao, T Hattori, S Kubota, T Kuboki, M Takigawa

    JOURNAL OF BONE AND MINERAL RESEARCH   20 ( 9 )   S197 - S197   2005年9月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER SOC BONE & MINERAL RES  

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  • Connective tissue growth factor (CTGF/CCN2) enhanced human bone marrow stromall cell attachment in vitro and migration and survival of the cells in a hydroxyapatite scaffold in vivo.

    M Ono, W Sonoyama, K Akiyama, T Fujisawa, T Nishida, M Takigawa, T Kuboki

    JOURNAL OF BONE AND MINERAL RESEARCH   20 ( 9 )   S203 - S204   2005年9月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER SOC BONE & MINERAL RES  

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  • 岡山大学歯学部1年次学生に対するテュートリアル教育の成果 : 平成15年度プログラムの多面的分析.

    久保田聡, 完山 学, 吉田登志子, 十川紀夫, 山本龍生, 平田あずみ, 美藤純弘, 十川千春, 市川博之, 鈴木康司, 衣田圭宏, 丸尾幸憲, 宮脇卓也, 香川智正, 西村英紀, 窪木拓男, 松村誠士

    岡山歯学会雑誌   2005年

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  • 補綴の未来にいかに貢献するか

    窪木拓男

    補綴臨床   2005年

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  • 岡山大学歯学部1年次生におけるテュートリアル教育の問題点と今後の課題.

    完山 学, 久保田 聡, 市川博之, 十川紀夫, 平田あずみ, 美藤純弘, 岡本 信, 福永智広, 香川智正, 中山周子, 坂本友紀, 吉田登志子, 宮脇卓也, 西村英紀, 松村誠士, 窪木拓男

    岡山歯学会雑誌   2005年

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  • Intramuscular haemodynamics using near infra-red spectroscopy as a research strategy to understand chronic muscle pain pathophysiology

    T Kuboki, K Maekawa, G Clark

    SPECTROSCOPY-AN INTERNATIONAL JOURNAL   19 ( 1 )   27 - 36   2005年

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    記述言語:英語   出版者・発行元:IOS PRESS  

    Recently, localized intramuscular haemodynamic disturbance has been recognized as one of the cardinal features of a chronically painful muscle. To test this hypothesis, we have carried out several studies that have assessed intramuscular haemodynamics to understand more about haemodynamic alteration under conditions of an experimentally induced vasoreactive challenges in healthy controls and in patients with chronic muscle pain using near infra-red (NIR) spectroscopy. In addition, we have validated the NIR based haemodynamic signals by comparing against a water signal intensity change taken from a transverse T-2-weighted magnetic resonance image (MRI) of the muscles. As a result, we discovered that muscle pain patients have a diminished ability to vasodilate in painful muscle. We also provided powerful evidences that NIR data (total haemoglobin level), transcutaneously taken from the muscles in humans could be an analogue of intramuscular haemodynamics.

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  • The 1st Congress of Asian Academy of Osseointegration (AAO)に出席して

    窪木拓男, 大野光昭

    歯界展望   2005年

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  • 再生医療と歯科補綴学の接点.補綴治療における再生医療のニーズと現時点での研究動向.確実かつ質の高いインプラント治療をめざして.

    窪木拓男

    日本補綴歯科学会雑誌   49,4,576-586   2005年

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  • Movement of the lateral and medial poles of the working condyle during mastication in patients with unilateral posterior crossbite. 国際誌

    Shouichi Miyawaki, Yuko Tanimoto, Yoshiko Araki, Akira Katayama, Takuo Kuboki, Teruko Takano-Yamamoto

    American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics   126 ( 5 )   549 - 54   2004年11月

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    記述言語:英語   出版者・発行元:MOSBY-ELSEVIER  

    Patients with unilateral posterior crossbite often show reverse sequential jaw movement patterns on the frontal view during mastication on the crossbite side. Recent studies show that such patients are prone to suffer from temporomandibular joint (TMJ) disc displacement, particularly the lateral portion. The purpose of this study was to examine the movement of the lateral and medial poles of the working condyle during mastication in such patients. Subjects were 12 consecutive patients with unilateral posterior crossbites and without TMJ disc displacements and 12 normal subjects. An optoelectronic jaw-tracking system with 6 degrees of freedom was used to record the motion of the lateral and medial poles of the working condyle during mastication of standardized hard, gummy jelly. The data from the first 10 cycles were analyzed. The lateral and medial poles of the condyle on the crossbite side moved more in the medial direction and less in the lateral direction during mastication in the crossbite patients than the condyle in the normal subjects. The lateral pole of the working condyle moved more in the posterior and inferior directions and less in the anterior direction than the medial pole in all subjects. These results suggest that these condylar movements in patients with unilateral posterior crossbites might be related to the susceptibility to TMJ disc displacement, particularly the lateral portion.

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  • Regeneration of defects in the articular cartilage in rat knee joints by connective tissue growth factor hypertrophic chondrocyte-specific gene product 24 CCN family member 2 (CTGF/Hcs24/CCN2).

    T Nishida, S Kubota, T Kuboki, K Nakao, T Kushibiki, Y Tabata, M Takigawa

    JOURNAL OF BONE AND MINERAL RESEARCH   19   S216 - S216   2004年10月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER SOC BONE & MINERAL RES  

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  • Self-reported remission, difficulty, and satisfaction with nonsurgical therapy used to treat anterior disc displacement without reduction

    H Minakuchi, T Kuboki, K Maekawa, Y Matsuka, H Yatani

    ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS   98 ( 4 )   435 - 440   2004年10月

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    記述言語:英語   出版者・発行元:MOSBY, INC  

    Objective. The purpose of this study was to identify the appropriate treatment element for initial anterior disc displacement without reduction subjects.
    Study design. Sixty-nine consecutive patients with temporomandibular joint disc displacement without reduction confirmed on magnetic resonance images were randomly divided into 3 experimental treatment groups. The treatment of group 1 consisted of short-term nonsteroidal anti-inflammatory drugs and self-care instructions (palliative care group); group 2, nonsteroidal anti-inflammatory drugs, self-care instructions, and occlusal appliance and mobilization therapy (physical medicine group); and group 3, no treatment (control group). Outcomes were assessed by means of a 5-item questionnaire that evaluated (1) symptom improvement, (2) difficulty of treatment, and (3) satisfaction with treatment during the 8-week observation period.
    Results. Improvement scores in the palliative care group were significantly better than those in the physical medicine group or the no-treatment group. Satisfaction scores showed no significant difference among the 3 groups. Difficulty from treatment for the physical medicine group was significantly greater than that for other 2 groups.
    Conclusion. These data suggest that palliative care would be more appropriate as the initial therapy to treat painful anterior disc displacement without reduction.

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  • 歯胚発生初期におけるBMP-4によるCTGFの発現制御とその機能

    完山 学, 志茂 剛, 窪木 拓男, Pacifici Maurizio, 小山 英樹

    日本口腔科学会雑誌   53 ( 4 )   179 - 179   2004年9月

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    記述言語:日本語   出版者・発行元:(NPO)日本口腔科学会  

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  • Soluble tumour necrosis factor receptors in synovial fluids from temporomandibular joints with painful anterior disc displacement without reduction and osteoarthritis. 国際誌

    Junji Uehara, Takuo Kuboki, Takuo Fujisawa, Shunji Kojima, Kenji Maekawa, Hirofumi Yatani

    Archives of oral biology   49 ( 2 )   133 - 42   2004年2月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The objective of this study was to detect soluble-form tumour necrosis factor receptors (sTNFRs) in temporomandibular joint (TMJ) synovial fluid aspirates, and to compare the sTNFR concentrations between painful anterior disc displacement without reduction and osteoarthritis (ADDwoR/OA) and asymptomatic TMJs. Synovial fluid was sampled from the superior TMJ cavity of 11 painful ADDwoR/OA cases (mean age: 36.9 years) and 10 asymptomatic females (mean age: 24.7 years) by diluted aspiration. The concentrations of sTNFR-I and -II in the synovial fluid were measured using human sTNFR-I and -II enzyme-linked immunosorbent assays. The total protein concentrations in synovial fluids were measured using a bicinchoninic acid protein assay kit. All data were normalised to the total protein concentration of each sample.Two-way factorial analysis of variance and post hoc multiple comparison revealed that: (1). mean normalised sTNFR-I and -II concentrations were higher in TMJ synovial aspirates from ADDwoR/OA patients than from healthy controls; (2). in the ADDwoR/OA patients and the healthy controls, the sTNFR-I concentration in TMJ synovial aspirates was higher than the sTNFR-II concentration; and (3). high TMJ synovial aspirate sTNFR-II seemed to be associated with less TMJ pain and a less restricted range of mouth opening in the ADDwoR/OA patients. The concentrations of sTNFRs in TMJ synovial fluid are higher in the presence of painful ADDwoR/OA, which could modulate intracapsular inflammation.

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  • 結合組織成長因子CTGF/CCN2 によるラット関節軟骨の再生 査読

    久保田聡, 西田崇, 小島俊司, 窪木拓男, 櫛引俊宏, 田畑泰彦, 滝川正春

    第3 回日本再生医療学会(2004.3.23-25. 千葉)   2004年

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  • 少数歯欠損患者の治療オプションの選択 ー義歯と口腔インプラント治療の比較ー.

    荒川 光, 窪木拓男

    日本歯科評論   64,4,59-65   2004年

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  • 歯科医療の全身健康へのかかわりをいかにひもとくか ーもう1つのパラダイムシフトを目前にしてー.

    窪木拓男

    日本歯科補綴学会雑誌   2004年

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  • 軟骨の医学 軟骨とメカニカルストレス ー発生、成長、病態、治療などとの観点からー.

    藤澤拓生, 窪木拓男, 滝川正春

    Clinical Calcium   14,7,1049-1055   2004年

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  • 市販カリエスリスクテストを用いた成人唾液中のう蝕原性細菌数検査の信頼性

    杉山 英樹, 窪木 拓男, 水口 一, 上原 淳二, 峯 篤史, 矢谷 博文, 山下 敦

    日本補綴歯科学会雑誌   47 ( 5 )   807 - 813   2003年12月

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    記述言語:日本語   出版者・発行元:(公社)日本補綴歯科学会  

    近年になり開発されたOrion製Dentocult(Dentocult)は,う蝕活動性に関連する複数の因子を効率よく検査することを可能としたが,使用法が若干煩雑であった.これを改善し,より簡便で高い診断精度を得るためにIvoclar Vivadent製CRT(CRT)が開発された.しかし,これらの検査法は幼年期から青年期までの若年層を対象に開発されたものであり,成人における信頼性は明らかではない.そこで,DentocultならびにCRTの刺激唾液中S.mutans菌数及びLactobacilli菌数測定検査の検者内・検者間,ならびに複数回行った検査結果の信頼性を比較し,成人被検者における臨床上の有用性を考察した.両測定法とも,臨床応用可能な信頼性を示すことが示唆された

    DOI: 10.2186/jjps.47.807

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  • Fiber post with resin cement coating: study of mechanical properties

    Yoshiga, SR, A Mine, J Tanaka, K Suzuki, T Kuboki, A Yamashita

    JOURNAL OF DENTAL RESEARCH   82   427 - 427   2003年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • ヒト歯髄由来間葉系幹細胞の細胞接着ならびに増殖に対する各種成長因子の影響

    園山 亘, 藤沢 拓生, 大野 充昭, 志茂 剛, 西田 崇, 滝川 正春, 窪木 拓男

    日本再生歯科医学会誌   1 ( 1 )   77 - 77   2003年12月

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    記述言語:日本語   出版者・発行元:日本再生歯科医学会  

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  • A repetitive, steady mouth opening induced an osteoarthritis-like lesion in the rabbit temporomandibular joint

    T Fujisawa, T Kuboki, T Kasai, W Sonoyama, S Kojima, J Uehara, C Komori, H Yatani, Hattori, I, M Takigawa

    JOURNAL OF DENTAL RESEARCH   82 ( 9 )   731 - 735   2003年9月

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    記述言語:英語   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

    Although excessive mechanical stress is assumed to be one of the factors contributing to pathogenesis of temporomandibular joint (TMJ) osteoarthritis (OA), no pure mechanical-stress-induced OA model has been developed without surgical manipulation or puncture of the joint cavity. The purpose of this study was to establish a genuine mechanical-stress-induced OA model of the rabbit TMJ. In the experimental rabbits, repetitive, forced jaw-opening, 3 hrs/day for 5 days, was applied with the use of a general anesthesia protocol. By histological assessment of the TMJ articular tissues, partial eburnation of the articular cartilage, reactive marginal proliferation of the articular cartilage chondrocytes, and nested proliferation of chondrocytes in the subchondral bone area were observed at 7 days after the repetitive, forced-jaw-opening period. These results suggest that the repetitive, forced-jaw-opening protocol without surgical intervention can induce evident OA-like lesions in the rabbit TMJ, and this OA model may greatly contribute to the elucidation of the cartilage degradation mechanism in TMJ OA.

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  • Cbfa1/Runx2 gene expression in articular chondrocytes of the mice temporomandibular and knee joints in vivo. 国際誌

    Takuo Kuboki, Manabu Kanyama, Tohru Nakanishi, Kentaro Akiyama, Kumiko Nawachi, Hirofumi Yatani, Kazuo Yamashita, Teruko Takano-Yamamoto, Masaharu Takigawa

    Archives of oral biology   48 ( 7 )   519 - 25   2003年7月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Healthy articular cartilage is thought to be maintained by the modulation of Cbfa1 expression, although little is currently known about Cbfa1 expression in such tissues. Therefore, we examined in vivo Cbfa1 transcript levels in the temporomandibular (TM) and knee joints of 3- and 10-week-old male ICR mice (weighing 50-70 g). A digoxigenin-11-UTP-labeled single-stranded RNA probe (0.6 kbp PstI-HindIII fragment of the 3' of untranslated region in exon 8 of mouse Cbfa1 cDNA) was prepared and in situ hybridization was performed on paraffin-embedded TM and knee joint sections. The antisense probe detected Cbfa1 transcripts in prehypertrophic chondrocytes, but not in the articular surface layer chondrocytes of 3- and 10-week-old mice TMJs. Despite the intense Cbfa1 expression in prehypertrophic chondrocytes, articular surface layer chondrocytes of the knee joints expressed low and undetectable level of Cbfa1 in the 3- and 10-week-old mice, respectively. These results indicate that Cbfa1 are highly expressed in the prehypertrophic chondrocytes presumably for articular tissue remodeling during the entire lifespan of the mouse, whereas Cbfa1 expression is suppressed in the articular surface chondrocytes in the adult mouse TM and knee joints to obtain the permanent cartilage phenotype.

    DOI: 10.1016/S0003-9969(03)00088-8

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  • 超音波洗浄や滅菌処置が歯科用金属アレルギーの非破壊的原因金属同定結果に及ぼす影響

    峯 篤史, 窪木 拓男, 長岡 紀幸, 土本 洋平, 園山 亘, 前川 賢治, 矢谷 博文

    岡山歯学会雑誌   22 ( 1 )   183 - 188   2003年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

    研磨用ポイント(WP)で歯科鋳造用銀合金及び陶材焼付用白色合金を研磨し,WPの表面を走査型電子顕微鏡で観察した.表面には金属付着が観察され,元素分析により銀合金においてはC,O,Al,Zn,Agが,白色合金ではC,O,Al,Cr,Niが検出された.又,試料を無処理群,蒸留水浸漬群,超音波処理群に分け,同様に金属分析を行ったところ,超音波処理群で銀合金の主成分であるAg,白色合金の主成分のNi,Crが検出され,無処理群,蒸留水浸漬群と同等の結果であった.銀合金でのZnは超音波処理群で全試料の1/3に,他の2群では2/3に検出された.次に試料を薬液滅菌群とオートクレーブ群に分け,超音波洗浄後金属分析を行った.両群とも主成分が検出されたが,銀合金のZnはオートクレーブ群で1/3のみ検出され,薬液滅菌群では検出されなかった.又,両群とも無処理及び蒸留水浸漬に比較してCが検出されにくかった

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  • Tyrosine kinase-type receptors erbB4 and m-csfr/c-fms gene expression in chondrocytes.

    K. Nawachi, S. Kubota, M. Inoue, T. Nishida, T. Kuboki, T. Nakanishi, H. Yatani, M. Takigawa

    JOURNAL OF DENTAL RESEARCH   82   B357 - B357   2003年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • Effect of Er : YAG laser irradiation on compressive properties of dental hard tissues.

    A. Mine, K. Suzuki, T. Kuboki, H. Yatani

    JOURNAL OF DENTAL RESEARCH   82   B377 - B377   2003年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • Responses of hypothalamic-pituitary-adrenal axis to cold pressor stimulation.

    K. Suzuki, K. Maekawa, T. Kuboki, H. Yatani

    JOURNAL OF DENTAL RESEARCH   82   B296 - B296   2003年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • Daily activity limitation and subcategories of TMJ internal derangements.

    T. Kuroi, Y. Nakazawa, K. Maekawa, T. Kuboki, H. Yatani

    JOURNAL OF DENTAL RESEARCH   82   B231 - B231   2003年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • Plasma beta-endorphin concentration and pain threshold changes upon CP stimulation.

    K. Maekawa, K. Suzuki, Y. Nakazawa, T. Kuboki, H. Yatani

    JOURNAL OF DENTAL RESEARCH   82   B160 - B160   2003年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • Regeneration of defects in the articular cartilage in rat knee joints by connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24)

    T Nishida, S Kubota, S Kojima, T Kuboki, T Kushibiki, Y Tabata, M Takigawa

    BONE   32 ( 5 )   S101 - S101   2003年5月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE INC  

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  • 片側臼歯部交叉こう合患者の顎関節空隙について

    荒木佳子, 宮脇正一, 谷本裕子, 片山朗, 窪木拓男, 山本照子

    日本顎関節学会雑誌   15 ( 1 )   137   2003年4月

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  • 正常こう合者のそしゃく運動時における下顎頭内外側極点の運動について

    片山朗, 宮脇正一, 谷本裕子, 荒木佳子, 窪木拓男, 山本照子

    日本顎関節学会雑誌   15 ( 1 )   136   2003年4月

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  • レーザーを用いたう蝕治療 招待

    峯 篤史, 鈴木一臣, 矢谷博文, 窪木拓男

    う蝕治療のミニマルインターベンション(監修 吉山 昌宏,桃井 保子)   180 - 183   2003年1月

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    記述言語:日本語   出版者・発行元:クインテッセンス出版株式会社, 東京  

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  • Function of β2-adrenergic receptors in chronic localized myalgia. 国際誌

    Maekawa K, Kuboki T, Inoue E, Inoue-Minakuchi M, Suzuki K, Yatani H, Clark GT

    J Orofac Pain   17 ( 2 )   140 - 4   2003年

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    記述言語:英語  

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  • 実際の患者の問題点に基づいた少数歯欠損機能回復法に関する問題発見解決型(チュートリアル)教育の試み.

    窪木拓男, 園山 亘, 荒川 光, 前川賢治, 鈴木康司, 藤沢拓生, 小島俊司, 笠井昭夫, 上原淳二, 杉山英樹, 完山 学, 矢谷博文, 山下 敦

    岡山歯学会雑誌   2003年

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  • Use of a porcelain laminate veneer for esthetic improvement after orthodontic treatment.

    Kuroda S, Kuboki T, Katayama A, Takano-Yamamoto T

    Orthodontic Waves   2003年

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  • 筋筋膜疼痛の関連痛として現れた非歯原性歯痛(筋筋膜性歯痛)の一症例.

    前川賢治, 窪木拓男, 矢谷博文

    岡山歯学会雑誌   2003年

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  • 少数歯欠損患者への治療オプション提示に必要なコミュニケーション能力評価の試み--客観的臨床能力試験(OSCE)を用いた卒前臨床実習評価?-.

    荒川 光, 窪木拓男, 水口 一, 前川賢治, 鈴木康司, 藤沢拓生, 園山 亘, 笠井昭夫, 小島俊司, 上原淳二, 完山 学, 矢谷博文

    岡山歯学会雑誌   2003年

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  • 口唇・口蓋裂患者の顎裂骨移植部にBronemarkインプラントを応用した2例.

    藤井崇史, 植野高章, 三島克章, 香川智正, 石田展久, 澤木康一, 加納みわ, 平田晃弘, 小島俊司, 窪木拓男, 菅原利夫

    岡山歯学会雑誌   2003年

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  • 早期発症型歯周炎患者における自家骨移植とインプラントによる治療経験.

    加納みわ, 植野高章, 石田展久, 藤井崇史, 平田晃弘, 吉本智人, 本多浩三, 澤木康一, 近藤誠二, 福永城司, 荒川 光, 窪木拓男, 菅原利夫

    岡山歯学会雑誌   2003年

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  • 骨膜下インプラント撤去後の高度な顎堤吸収症例に対してインプラント治療を行った1例.

    園山 亘, 真野隆充, 窪木拓男, 上山吉哉, 鈴木秀典, 山田庸介, 松村智弘

    岡山歯学会雑誌   2003年

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  • Signal intensity changes in T2-weighted MR image of the human trapezius muscle upon cold pressor stimulation

    M Inoue-Minakuchi, T Kuboki, K Maekawa, Y Yanagi, E Inoue, T Wakasa, K Kishi, H Yatani, GT Clark

    DENTOMAXILLOFACIAL RADIOLOGY   31 ( 6 )   350 - 354   2002年11月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Objective: The ability to measure hemodynamics of skeletal muscle proper is one of the major goals for muscle pain researchers. The aim of the present study was to evaluate the ability of signal intensity (SI) in T2-weighted trapezius muscle magnetic resonance imaging (MRI) to detect intramuscular hemodynamic changes during cold pressor stimulation (CPS).
    Materials and methods: Fifteen healthy volunteers (mean age, 25.9 +/- 2.1 years) participated in this study. T2-weighted MRI was acquired using a 1.5 tesla MR unit with a body array coil. The slice level was set perpendicular to the muscle long axis at the mid-point of the horizontal portion of the right trapezius muscle. Cold pressor stimulation (4degreesC) was applied to each subject's right foot and ankle for 2 min. The SI changes were recorded continuously for 7 min before, 2 min during, and 6 min after withdrawal of cold pressor stimulation. Six of these subjects also underwent a mock-CPS trial.
    Results: The mean SI level in T2-weighted trapezius muscle MRI significantly increased during CPS (P&lt;0.0001, one way repeated measure ANOVA) and returned to the baseline level after cold pressor withdrawal. No statistically significant signal changes were observed across the mock-CPS trial subjects. These findings are identical to the cold pressor-induced hemodynamic changes documented in the trapezius muscle by near-infrared spectroscopy evaluation.
    Conclusions: Sl measurement in T2-weighted trapezius muscle MRI is sufficiently sensitive to detect intramuscular hemodynamic changes during CPS.

    DOI: 10.1038/sj.dmfr.4600721

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  • Tyrosine kinase-type receptor ErbB4 in chondrocytes: interaction with connective tissue growth factor and distribution in cartilage. 国際誌

    Kumiko Nawachi, Miho Inoue, Satoshi Kubota, Takashi Nishida, Gen Yosimichi, Tohru Nakanishi, Manabu Kanyama, Takuo Kuboki, Hirofumi Yatani, Tomoichiro Yamaai, Masaharu Takigawa

    FEBS letters   528 ( 1-3 )   109 - 13   2002年9月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    In order to identify receptor molecules that participate in the growth and differentiation of chondrocytes, we cloned a number of cDNA fragments from HCS-2/8 chondrocytic cells, by using tyrosine kinase-specific primers for amplification. The mRNA expression of one such receptor, ErbB4, was increased by connective tissue growth factor/hypertrophic chondrocyte-specific gene product (CTGF/Hcs24), which promotes all stages of the endochondral ossification in vitro. ErbB4 expression was observed through all stages of chondrocytic differentiation in vitro, corresponding to the wide distribution of CTGF/Hcs24 target cells. Furthermore, positive signals for erbB4 mRNA were detectable throughout most populations of chondrocytes, in growth and articular cartilage in vivo. These results demonstrate for the first time that ErbB4 is expressed in chondrocytes and may play some roles in chondrocytic growth and differentiation along with CTGF/Hcs24.

    DOI: 10.1016/S0014-5793(02)03263-5

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  • Effects of IL-1beta and LPS on CTGF expression in mouse-derived odontoblast-like cells, MDPC-23.

    W Sonoyama, T Kuboki, T Fujisawa, T Eguchi, J Uehara, H Yatani, M Takigawa

    JOURNAL OF BONE AND MINERAL RESEARCH   17   S327 - S327   2002年9月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER SOC BONE & MINERAL RES  

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  • CTGF/Hcs24, a hypertrophic chondrocyte-specific gene product, stimulates proliferation and differentiation but not hypertrophy of cultured articular chondrocytes.

    T Nishida, S Kubota, T Nakanishi, T Kuboki, G Yosimichi, S Kondo, M Takigawa

    JOURNAL OF BONE AND MINERAL RESEARCH   17   S180 - S181   2002年9月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER SOC BONE & MINERAL RES  

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  • Novel cis-element TRENDIC that enhance connective tissue growth factor (ctgf) gene expression in chondrocytic HCS-2/8.

    T Eguchi, S Kubota, S Kondo, Y Mukudai, T Kuboki, H Yatani, M Takigawa

    JOURNAL OF BONE AND MINERAL RESEARCH   17   S224 - S224   2002年9月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER SOC BONE & MINERAL RES  

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  • Quality of life assessment in patients with implant-supported and resin-bonded fixed prosthesis for bounded edentulous spaces. 国際誌

    Wataru Sonoyama, Takuo Kuboki, Soichiro Okamoto, Hidenori Suzuki, Hikaru Arakawa, Manabu Kanyama, Hirofumi Yatani, Atsushi Yamashita

    Clinical oral implants research   13 ( 4 )   359 - 64   2002年8月

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    記述言語:英語   出版者・発行元:BLACKWELL MUNKSGAARD  

    OBJECTIVES: Dental implants have become increasingly popular in the prosthetic rehabilitation of patients with bounded edentulous spaces. Oral condition-related quality of life (QOL) levels have rarely been assessed in these patients. MATERIAL AND METHODS: Two groups of subjects with bounded edentulous spaces were studied: an implant-supported fixed prosthesis group (11 patients) and a resin-bonded fixed prosthesis group (33 patients). The two groups were well matched in terms of sex, age, missing units and location of missing units. The patients were requested to answer a self-administered QOL questionnaire with two major subscales - oral condition- and general condition-related QOL scores. The test-retest reliability of each question was pre-examined and found acceptable (mean Spearman rank correlation coefficient was 0.55 +/- 0.16). Mean QOL score differences between the two groups were analyzed by the Mann-Whitney U-test. RESULTS: Mean oral condition-related QOL scores of the implant-supported and resin-bonded fixed prosthesis groups were 87.8 +/- 9.5 and 87.1 +/- 12.3% (P = 0.85), and mean general condition-related QOL scores were 73.8 +/- 14.8 and 71.6 +/- 15.2% (P = 0.95), respectively. No significant QOL differences between the two groups were observed in the two subscales. CONCLUSION: In patients with bounded edentulous spaces, multidimensional QOL levels of patients with an implant-supported fixed prosthesis do not exceed those of patients with a resin-bonded fixed prosthesis in a short follow-up period.

    DOI: 10.1034/j.1600-0501.2002.130403.x

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  • Reply to commentaries

    GT Clark, K Maekawa, T Kuboki

    JOURNAL OF PAIN   3 ( 4 )   270 - 271   2002年8月

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    記述言語:英語   出版者・発行元:CHURCHILL LIVINGSTONE  

    DOI: 10.1054/jpai.2002.125926

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  • Intramuscular hypoperfusion, adrenergic receptors, and chronic muscle pain. 国際誌

    Kenji Maekawa, Glenn T Clark, Takuo Kuboki

    The journal of pain : official journal of the American Pain Society   3 ( 4 )   251 - 60   2002年8月

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    記述言語:英語   出版者・発行元:CHURCHILL LIVINGSTONE  

    Despite a high prevalence of chronic muscle pain disorders such as fibromyalgia and regional myofascial pain, there is still limited knowledge about the factors that initiate and perpetuate these pain states. Although there are also likely to be downstream neuropathic changes in the central nervous system and spinal cord that sustain and exacerbate the pain states known as fibromyalgia, the focus of this critical review is on studies that examined the connection between both fibromyalgia and regional myofascial pain and sympathetic function. Specifically, we looked at studies that described Raynaud-like symptoms, cardiovascular dysfunction and altered intramuscular perfusion in chronic muscle pain. Our analysis showed that although the first 2 phenomena were intermittently present, a prominent and consistent feature for regional myofascial pain and to a lesser degree for fibromyalgia was intramuscular hypoperfusion. Several hypotheses can be offered why this hypoperfusion exists, and additional studies comparing and contrasting these theories are needed. This review focuses on one of these theories, namely, agonist-induced beta-adrenergic receptor desensitization as an explanatory model for hypoperfusion. What cannot be done at this time and is needed in the future is to compare and contrast to what degree the regional muscle pain disorder (myofascial) is similar or different from the more generalized disorder (fibromyalgia).

    DOI: 10.1054/jpai.2002.125923

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  • A novel cis-element that enhances connective tissue growth factor gene expression in chondrocytic cells. 国際誌

    Takanori Eguchi, Satoshi Kubota, Seiji Kondo, Takuo Kuboki, Hirofumi Yatani, Masaharu Takigawa

    Biochemical and biophysical research communications   295 ( 2 )   445 - 51   2002年7月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    To clarify the chondrocyte-specific regulatory mechanism of connective tissue growth factor (ctgf) gene expression, we analyzed the functionality and DNA-protein interaction of the CTGF promoter. Comparative luciferase assay of the CTGF promoter deletion mutants among HCS-2/8 chondrocytic cells and fibroblastic cells revealed that a 110-bp region in the promoter was crucial for the HCS-2/8-specific transcriptional enhancement. Subsequent competitive gel shift assay revealed that transcription factors in HCS-2/8 nuclei bound to a 60-bp portion in the corresponding region. Relative luciferase activity from a CTGF promoter with mutant TGF-beta response element (TbRE) was 16.9% lower than that from an intact promoter. On the other hand, relative luciferase activity from a CTGF promoter with 4bp point mutations at 30bp upstream of the TbRE was 47.7% lower than that from the intact one. The binding activity of HCS-2/8 nuclear factor(s) to the sequence over the 4-bp was remarkably higher than that of any nuclear extract from other types of cells. Therefore, we entitled the sequence 'TRENDIC', a transcription enhancer dominant in chondrocytes, which stands for a novel enhancer for chondrocyte-specific CTGF gene expression.

    DOI: 10.1016/S0006-291X(02)00700-3

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  • Reply to commentaries

    J de Peyer

    PSYCHOANALYTIC DIALOGUES   12 ( 4 )   545 - 549   2002年7月

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    記述言語:英語   出版者・発行元:ANALYTIC PRESS  

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  • CTGF/Hcs24, a hypertrophic chondrocyte-specific gene product, stimulates proliferation and differentiation, but not hypertrophy of cultured articular chondrocytes. 国際誌

    Takashi Nishida, Satoshi Kubota, Tohru Nakanishi, Takuo Kuboki, Gen Yosimichi, Seiji Kondo, Masaharu Takigawa

    Journal of cellular physiology   192 ( 1 )   55 - 63   2002年7月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    We previously reported that connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) stimulated the proliferation and differentiation of rabbit growth cartilage (RGC) cells in vitro. In this study, we investigated the effects of CTGF/Hcs24 on the proliferation and differentiation of rabbit articular cartilage (RAC) cells in vitro. RAC cells transduced by recombinant adenoviruses generating mRNA for CTGF/Hcs24 synthesized more proteoglycan than the control cells. Also, treatment of RAC cells with recombinant CTGF/Hcs24 (rCTGF/Hcs24) increased DNA and proteoglycan syntheses in a dose-dependent manner. Northern blot analysis revealed that the rCTGF/Hcs24 stimulated the gene expression of type II collagen and aggrecan core protein, which are markers of chondrocyte maturation, in both RGC and RAC cells. However, the gene expression of type X collagen, a marker of hypertrophic chondrocytes, was stimulated by rCTGF/Hcs24 only in RGC cells, but not in RAC cells. Oppositely, gene expression of tenascin-C, a marker of articular chondrocytes, was stimulated by rCTGF/Hcs24 in RAC cells, but not in RGC cells. Moreover, rCTGF/Hcs24 effectively increased both alkaline phosphatase (ALPase) activity and matrix calcification of RGC cells, but not of RAC cells. These results indicate that CTGF/Hcs24 promotes the proliferation and differentiation of articular chondrocytes, but does not promote their hypertrophy or calcification. Taken together, the data show that CTGF/Hcs24 is a direct growth and differentiation factor for articular cartilage, and suggest that it may be useful for the repair of articular cartilage.

    DOI: 10.1002/jcp.10113

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  • 片側臼歯部交叉こう合患者におけるそしゃく運動時の下顎頭運動について

    宮脇正一, 谷本裕子, 菅原康代, 荒木佳子, 窪木拓男, 山本照子

    日本顎関節学会雑誌   14 ( 1 )   126 - 127   2002年4月

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    記述言語:日本語  

    J-GLOBAL

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  • CTGF upregulation observed in the rat tooth extraction sockets.

    M Kanyama, T Kuboki, K Akiyama, F Miyauchi, K Nawachi, H Yatani, S Kubota, T Nakanishi, M Takigawa

    JOURNAL OF DENTAL RESEARCH   81   A107 - A107   2002年3月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • Temporomandibular joint space distribution and prognosis of ADDwor patients.

    H Minakuchi, Y Azuma, K Maekawa, T Kuboki, H Yatani

    JOURNAL OF DENTAL RESEARCH   81   A212 - A212   2002年3月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • Beta-adrenergic receptor function in chronic localized myalgia subjects.

    K Maekawa, E Inoue, M Inoue-Minakuchi, K Suzuki, T Kuboki, H Yatani, GT Clark

    JOURNAL OF DENTAL RESEARCH   81   A296 - A296   2002年3月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • Cold-pressor-induced signal intensity change in the T2-weighted trapezius muscle MRI.

    M Inoue-Minakuchi, Y Yanagi, K Maekawa, T Kuboki, E Inoue, K Kishi, H Yatani, GT Clark

    JOURNAL OF DENTAL RESEARCH   81   A131 - A131   2002年3月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • Effects of proinflammatory factors on CTGF expression in odontoblast-like cells

    W Sonoyama, T Kuboki, T Fujisawa, T Eguchi, J Uehara, S Takashiba, H Yatani, M Takigawa

    JOURNAL OF DENTAL RESEARCH   81   A155 - A155   2002年3月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • Disturbed hemodynamic responses in painful trapezius muscle to CP stimulation.

    T Kuboki, CO Acero, K Maekawa, M Inoue-Minakuchi, H Yatani, GT Clark

    JOURNAL OF DENTAL RESEARCH   81   A316 - A316   2002年3月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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  • 外傷に対する歯牙欠損に対しインプラント治療を施行した症例の検討

    田村章規, 真野隆充, 上山吉哉, 岸本晃治, 山田庸介, 新谷 悟, 完山 学, 荒川 光, 園山 亘, 窪木拓男, 矢谷博文, 松村智弘

    岡山歯学会雑誌   2002年

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  • Is there a relation between intramuscular hypoperfusion and chronic muscle pain?

    Thomas Graven-Nielsen, Lars Arendt-Nielsen, Glenn T. Clark, Kenji Maekawa, Takuo Kuboki

    Journal of Pain   3 ( 4 )   261 - 271   2002年

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    記述言語:英語   掲載種別:速報,短報,研究ノート等(学術雑誌)  

    DOI: 10.1054/jpai.2002.125927

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  • The search for the cause of persistent muscle pain

    Christian S. Stohler, Glenn T. Clark, Kenji Maekawa, Takuo Kuboki

    Journal of Pain   3 ( 4 )   268 - 271   2002年

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    記述言語:英語   掲載種別:速報,短報,研究ノート等(学術雑誌)  

    DOI: 10.1054/jpai.2002.125924

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  • オッセオインテグレーションの獲得と維持からみた口腔インプラントの生存に関する疫学調査

    荒川 光, 窪木拓男, 完山 学, 園山 亘, 小島俊司, 矢谷博文, 植野高章, 高木 慎, 菅原利夫, 真野隆充, 松村智弘

    日本口腔インプラント学会雑誌   2002年

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  • 初診時アンケートにおける顎関節症患者の訴えと細病態分類

    鈴木康司, 前川賢治, 窪木拓男, 矢谷博文

    日本補綴歯科学会雑誌   2002年

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  • Do we know enough to put forward a unifying hypothesis?

    Siegfried Mense, Glenn T. Clark, Kenji Maekawa, Takuo Kuboki

    Journal of Pain   3 ( 4 )   264 - 271   2002年

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    記述言語:英語   掲載種別:速報,短報,研究ノート等(学術雑誌)  

    DOI: 10.1054/jpai.2002.125925

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  • 骨軟骨組織修復における結合組織成長因子CTGF/Ecogeninの役割.

    西田 崇, 小島俊司, 久保田聡, 窪木拓男, 田畑泰彦, 櫛引俊宏, 中西 徹, 滝川正春

    第4回大阪組織工学研究セミナー.   2002年

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