2021/04/08 更新

写真a

サイトウ ノボル
齋藤 昇
SAITOU Noboru
所属
グローバル人材育成院 教授
職名
教授
外部リンク

学位

  • 農学博士 ( 名古屋大学 )

研究キーワード

  • ウズラ

  • 性分化

  • ニワトリ

  • 生殖腺

  • ミューラー管

  • ウオルフ管

研究分野

  • ライフサイエンス / 獣医学

  • ライフサイエンス / 獣医学

  • ライフサイエンス / 獣医学

  • ライフサイエンス / 動物生産科学

  • ライフサイエンス / 動物生産科学

学歴

  • 名古屋大学   Graduate School, Division of Agriculture  

    - 1985年

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  • 名古屋大学    

    - 1985年

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    国名: 日本国

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  • 東邦大学   理学部   生物学科

    - 1980年

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    国名: 日本国

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  • 東邦大学   Faculty of Science  

    - 1980年

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経歴

  • 岡山大学   大学院環境生命科学研究科   教授

    2012年8月 - 現在

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  • 名古屋大学   大学院生命農学研究科   准教授

    2007年 - 2012年

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  • 名古屋大学大学院生命農学研究科 助教授   Graduate School of Bioagricultural Sciences

    2001年 - 2007年

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  • 名古屋大学大学院生命農学研究科 助手   Graduate School of Bioagricultural Sciences

    1999年 - 2001年

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  • ドイツ連邦農業研究センター・小動物研究所 フンボルト財団リサーチフェロー

    1993年 - 1994年

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  • 名古屋大学農学部 助手   School of Agricultural Sciences

    1989年 - 1999年

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  • 日本学術振興会 特別研究員

    1989年

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  • 名古屋大学大学院農学研究科 研究生

    1988年 - 1989年

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  • 米国アーカンソ大学医学部 ポストドクトラルフェロー

    1986年 - 1988年

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  • 名古屋大学大学院農学研究科 研究生

    1985年 - 1986年

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▼全件表示

所属学協会

委員歴

  • 日本家禽学会   評議委員  

    2008年 - 現在   

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    団体区分:学協会

    日本家禽学会

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  • 日本家禽学会   編集委員  

    2000年 - 2009年   

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    団体区分:学協会

    日本家禽学会

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論文

  • Expression of aquaporin 4 in the chicken oviduct following tamoxifen treatment. 査読

    Socha JK, Saito N, Wolak D, Sechman A, Hrabia A

    Reproduction of domestic Animal doi: 10.1111/rda.13248.   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Expression of aquaporin 4 in the chicken ovary in relation to follicle development 査読

    M. Nowak, M. Grzesiak, N. Saito, M. Kwasniewska, A. Sechman, A. Hrabia

    REPRODUCTION IN DOMESTIC ANIMALS52 ( 5 ) 857 - 864   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    In the mammalian ovary, aquaporins (AQPs) are thought to be involved in the regulation of fluid transport within the follicular wall and antrum formation. Data concerning the AQPs in the avian ovary is very limited. Therefore, the present study was designed to examine whether the AQP4 is present in the chicken ovary, and if so, what is its distribution in the ovarian compartment of the laying hen. Localization of AQP4 in the ovarian follicles at different stage of development was also investigated. After decapitation of hens the stroma with primordial follicles and white (1-4 mm), yellowish (4-8 mm), small yellow and the three largest yellow pre-ovulatory follicles F3-F1 (F3 < F2 < F1; 20-36 mm) were isolated from the ovary. The granulosa and theca layers were separated from the pre-ovulatory follicles. The AQP4 mRNA and protein were detected in all examined ovarian compartments by the real-time PCR and Western blot analyses, respectively. The relative expression of AQP4 was depended on follicular size and the layer of follicular wall. It was the lowest in the granulosa layer of pre-ovulatory follicles and the highest in the ovarian stroma as well as white and yellowish follicles. Along with approaching of the largest follicle to ovulation the gradual decrease in AQP4 protein level in the granulosa layer was observed. Immunoreactivity for AQP4 was present in the granulosa and theca cells (theca interna >= theca externa > granulosa). The obtained results suggest that AQP4 may take part in the regulation of water transport required for follicle development in the chicken ovary.

    DOI: 10.1111/rda.12990

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  • The muscular dystrophic chicken is hypernatremic 査読

    N. Saito, H. Hirayama, K. Yoshimura, Y. Atsumi, M. Mizutani, K. Kinoshita, A. Fujiwara, T. Namikawa

    BRITISH POULTRY SCIENCE58 ( 5 ) 506 - 511   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    1. The E3 ubiquitin protein ligase 1 (WWP1) gene, the mutation of which causes muscular dystrophy in chickens, is expressed not only in the pectoral muscle, but also in a number of tissues such as the kidney. Therefore, this study examined some parameters related to kidney function in muscular dystrophic (MD) chickens.2. Plasma osmolality, Na+ and K+ concentrations, aldosterone levels, and the expression of aquaporin (AQP) 2, AQP3, and subunits of the amiloride-sensitive epithelial sodium channel (ENaC) were analysed in the kidneys of 5-week-old MD chickens and White Leghorn (WL) chickens under physiological conditions or after one day of water deprivation.3. Plasma osmolality, Na+ concentrations, and plasma aldosterone levels were significantly higher in MD chickens than in WL chickens. ENaC mRNA expression levels were lower in MD chickens than in WL chickens. AQP2 and AQP3 mRNA expression levels were similar in the two strains of chickens.4. Plasma osmolality correlated with aldosterone levels and AQP2 and ENaC mRNA levels in WL chickens. In MD chickens, plasma osmolality correlated with AQP2 mRNA levels, but not with plasma aldosterone or ENaC mRNA levels.5. These results suggest that neither water reabsorption nor the expression of AQP2 and AQP3 is impaired in MD chickens and that a WWP1 gene mutation may or may not directly induce an abnormality in Na+-reabsorption in the kidneys of MD chickens, potentially through ENaC.

    DOI: 10.1080/00071668.2017.1354356

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  • Female-biased sex difference in vasotocin-immunoreactive neural structures in the developing quail brain 査読

    Nicoletta Aste, Naoki Yoshioka, Emiko Sakamoto, Noboru Saito

    JOURNAL OF CHEMICAL NEUROANATOMY77   41 - 54   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The bed nucleus of the stria terminalis pars medialis (BSTM), medial preoptic nucleus (POM), and lateral septal region (LS) exhibit more vasotocin-immunoreactive (VT-ir) neural structures in male than in female adult quail. VT-ir cells and fibers in these regions are sensitive to gonadal steroids only in males. The insensitivity of adult female VT-ir neural structures to sex steroids is attributed to estradiol exposure during a critical period in embryonic life.
    Although the VT-ir system has been intensively examined in adult quail, information is limited in embryos and juveniles. Therefore, we herein investigated the development of VT-immunoreactive neural structures from embryonic day (E) 9 to adulthood with a particular focus on the BSTM, POM and LS of both sexes.
    VT-ir neural structures were more evident in female than in male embryos from E9 (BSTM and POM) and Eli (LS). This sex difference disappeared between E15 and post-hatch day 1 in the BSTM and POM, and during the first week of life in the LS. Male-biased sex differences in VT-ir structures appeared at puberty. Female-biased sexual dimorphism in the density of the VT-ir structures of BSTM was reflected by the stronger expression of VT mRNA in females than in males. However, the density of VT mRNA somata was comparable in the two sexes.
    The exposure of male embryos to estradiol resulted in the feminization of VT-ir neural structures in the BSTM, but not in the POM or LS at E11.
    Collectively, these results suggest that sex differences in VT-ir neural structures changes drastically throughout quail life. In embryos, endogenous estradiol may stimulate the expression of VT in females, resulting in a robust sex difference in VT-ir cells and fibers in favor of this sex. (C) 2016 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jchemneu.2016.05.002

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  • Vasotocin mRNA Expression is Sensitive to Testosterone and Oestradiol in the Bed Nucleus of the Stria Terminalis in Female Japanese Quail 査読

    N. Aste, E. Sakamoto, M. Kagami, N. Saito

    JOURNAL OF NEUROENDOCRINOLOGY25 ( 9 ) 811 - 825   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Vasotocin-producing parvocellular neurones in the medial part of the bed nucleus of the stria terminalis (BSTM) of many species of birds and mammals show sexual dimorphism and great plasticity in response to hormonal and environmental stimuli. In the BSTM of Japanese quail, vasotocin-immunoreactive neurones are visible and sensitive to testosterone exclusively in males. In males, gonadectomy decreases and testosterone restores vasotocin-immunoreactive cells and fibres by acting on vasotocin mRNA transcription. The insensitivity of female vasotocin-immunoreactive neurones to the activating effects of testosterone is the result of organisational effects of early exposure to oestradiol. Female quail also show vasotocin mRNA-expressing neurones in the BSTM, although it is not known whether the insensitivity of the vasotocinergic neurones to testosterone originates at the level of vasotocin gene transcription in this sex. Therefore, initially, the present study analysed the effects of acute treatment with testosterone on vasotocin mRNA expression in the BSTM of gonadectomised male and female quail using in situ hybridisation. Gonadectomy decreased (and a single injection of testosterone increased) the number of vasotocin mRNA-expressing neurones and intensity of the vasotocin mRNA hybridisation signal similarly in both sexes. Notably, testosterone increased vasotocin mRNA expression in ovariectomised females over that shown by intact quail. However, this treatment had no effect on vasotocin immunoreactivity. A second experiment analysed the effects of testosterone metabolites, oestradiol and 5-dihydrotestosterone, on vasotocin mRNA expression in female quail. Oestradiol (but not 5-dihydrotestosterone) fully mimicked the effects of testosterone on the number of vasotocin mRNA-expressing neurones and the intensity of the vasotocin mRNA hybridisation signal. Taken together, these results show, for the first time, that gonadal steroids strongly activate vasotocin mRNA expression in the BSTM of female quail.

    DOI: 10.1111/jne.12076

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  • Inheritance and Developmental Pattern of Cerebral Hernia in the Crested Polish Chicken 査読

    Keiji Yoshimura, Keiji Kinoshita, Makoto Mizutani, Yoichi Matsuda, Noboru Saito

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION318B ( 8 ) 613 - 620   2012年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    A number of crested chicken strains, such as the Polish chicken, exhibit a bony protuberance in the anterodorsal region of their skulls. The shape of their brain shows the anatomical peculiarity that is characterized by the upthrusting of cerebral hemispheres, called cerebral hernia. Some early works suggested that this phenotype may be caused by a genetic factor and any modifiers influencing the development of brain and/or skull. However, the causative gene and its formation mechanism are still unclear. The present study is aimed to analyze the inheritance and ontogenic process of cerebral hernia in the crested Polish chicken. Firstly, we constructed the resource family with the Polish chicken and PNP inbred strain. Genetic analysis of this family revealed that cerebral hernia is controlled by a single autosomal recessive gene and is closely associated with crest formation. Furthermore, our morphological analysis of brain structures in the progenies suggested that the significant enlargement of brain cavity at later stages of embryos, particularly after 15 days of incubation, may be the main cause of cerebral hernia. J. Exp. Zool. (Mol. Dev. Evol.) 9999B:613620, 2012. (c) 2012 Wiley Periodicals, Inc.

    DOI: 10.1002/jez.b.22464

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  • The mechanism underlying the central glucagon-induced hyperglycemia and anorexia in chicks 査読

    Kazuhisa Honda, Hiroshi Kamisoyama, Taku Uemura, Takashi Yanagi, Noboru Saito, Yohei Kurose, Kunio Sugahara, Kazuo Katoh, Shin Hasegawa

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY163 ( 3-4 ) 260 - 264   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    We investigated the mechanism underlying central glucagon-induced hyperglycemia and anorexia in chicks. Male 8-day-old chicks (Gallus gallus) were used in all experiments. Intracerebroventricular administration of glucagon in chicks induced hyperglycemia and anorexia from 30 min after administration. However, the plasma insulin level did not increase until 90 mm after glucagon administration, suggesting that glucose-stimulated insulin secretion from pancreatic beta cells may be suppressed by central glucagon. The plasma corticosterone concentration significantly increased from 30 min to 120 min after administration, suggesting that central glucagon activates the hypothalamic pituitary adrenal (HPA) axis in chicks. However, central administration of corticotropin-releasing factor (CRF), which activates the HPA axis in chicken hypothalamus, significantly reduced not only food intake but also plasma glucose concentration, suggesting that CRF and the activation of the HPA axis are related to the glucagon-induced anorexia but not hyperglycemia in chicks. Phentolamine, an et-adrenergic receptor antagonist, significantly attenuated the glucagon-induced hyperglycemia, suggesting that glucagon induced hyperglycemia at least partly via a-adrenergic neural pathway. Co-administration of phentolamine and a-helical CRF, a CRF receptor antagonist, significantly attenuated glucagon-induced hyperglycemia and anorexia. It is therefore likely that central administration of glucagon suppresses food intake at least partly via CRF-induced anorexigenic pathway in chicks. (C) 2012 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.cbpa.2012.08.005

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  • Analysis of Egg White Lysozyme Polymorphisms among Japanese Quail Populations in Japan and France 査読

    Si L. Myint, Takeshi Shimogiri, Keiji Kinoshita, Keijiro Nirasawa, Noboru Saitoh, Hisako Watanabe, Kotaro Kawabe, Yoshizane Maeda, Shin Okamoto

    JOURNAL OF POULTRY SCIENCE49 ( 2 ) 74 - 78   2012年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN POULTRY SCIENCE ASSOC  

    Japanese quail egg white lysozyme possesses two alleles (S and F). In our previous study, the F phenotype was more effective against bacteria than the S phenotype, whereas the female-derived S phenotype led to improved hatchability. In this study, we analyzed gene frequency of egg white lysozyme using Acid-PAGE and mismatch PCR-RFLP in 22 Japanese quail populations in Japan and compared with five populations in INRA, France. Genotyping of the lysozyme locus in 22 Japanese quail populations in Japan revealed that this locus is polymorphic in all populations except Nagoya University and Kagoshima University (LL and SS) populations. The S allele was a major allele with frequency from 0.69 to 1.00. In five French populations, it was polymorphic in all populations except AA population. The S allele was a major allele with frequency from 0.69 to 1.00. From these results, the lysozyme locus was polymorphic in the Japanese quails and the S allele was a major allele. The S allele was fixed in several populations which have been maintained as closed and small population.

    DOI: 10.2141/jpsa.011129

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  • Immunolocalization of aquaporin-4 in the brain, kidney, skeletal muscle, and gastro-intestinal tract of chicken 査読

    Keiji Yoshimura, Kanae Sugiura, Yasushige Ohmori, Nicoletta Aste, Noboru Saito

    CELL AND TISSUE RESEARCH344 ( 1 ) 51 - 61   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Aquaporin-4 (AQP4), a member of the water channel family, has high water permeability and multi-functional potentiality. Although an avian AQP4 homolog has recently been identified, its overall localization is still largely unknown. This study demonstrates the presence of AQP4 in several organs of chicken by using a specific chicken AQP4 antibody. Western blot analysis has revealed two bands of chicken AQP4 (30 and 32 kDa) in the brain, proventriculus, pectoral muscle, kidney, and ureter. The brain is the primary expression site of AQP4 in chicken. Immunohistochemical analysis of the brain has shown the highest AQP4 immunoreactivity around the cerebral ventricles, blood vessels, and the Purkinje cells. In peripheral organs, AQP4-immunoreactive elements have been observed in the ureter, glandular cells of the proventriculus, sarcolemma of the pectoral muscle, and the epithelium of the ceca and the rectum. Moreover, a heavily stained network of AQP4-immunoreactive fibers has been detected within the enteric plexuses.

    DOI: 10.1007/s00441-011-1134-5

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  • Novel Method of Gene Transfer in Birds: Intracytoplasmic Sperm Injection for Green Fluorescent Protein Expression in Quail Blastoderms 査読

    Shusei Mizushima, Soichi Takagi, Tamao Ono, Yusuke Atsumi, Akira Tsukada, Noboru Saito, Tomohiro Sasanami, Masaru Okabe, Kiyoshi Shimada

    BIOLOGY OF REPRODUCTION83 ( 6 ) 965 - 969   2010年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC STUDY REPRODUCTION  

    This study was conducted to establish a new method of avian transgenesis by intracytoplasmic sperm injection (ICSI). First, we evaluated the fertilization ability of quail oocytes after microinjection of Triton X-100 (TX-100)-treated quail sperm with PLCZ cRNA. The quail oocytes were cultured for 24 h, and blastoderm development was examined by histological observation. The TX-100 treatment induced damage to the quail sperm membrane and interfered with fertilization of oocytes injected with sperm. On the other hand, when quail oocytes were injected with TX-100-treated sperm and PLCZ cRNA simultaneously, 43.5% (10/23) of the oocytes developed into blastoderms. This rate of development was comparable to that for oocytes injected with sperm without TX-100 treatment but with PLCZ cRNA (6[42.9%] of 14). Second, we evaluated the rate of transduction of the enhanced green fluorescent protein (EGFP) gene in quail oocytes injected with TX-100-treated sperm and PLCZ cRNA. The EGFP expression was assessed by histological observation of fluorescence emission in the embryos. The intracytoplasmic injection of sperm without TX-100 treatment but with PLCZ cRNA and EGFP vector induced blastoderm development in 40% (4/10) of the oocytes, but those oocytes showed no fluorescence emission. In contrast, the intracytoplasmic injection of TX-100-treated sperm and PLCZ cRNA induced blastoderm development in 43.8% (7/16) of the oocytes, and, importantly, 85.7% (6/7) of oocytes showed fluorescence emission. In addition, PCR analysis detected GFP fragments in 50% (3/6) of GFP-expressing blastoderms. These results indicate that this ICSI method with additional treatments described herein may be the first step toward the production of transgenic birds.

    DOI: 10.1095/biolreprod.110.085860

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  • Distribution and sex differences in aromatase-producing neurons in the brain of Japanese quail embryos 査読

    Nicoletta Aste, Yumi Watanabe, Nobuhiro Harada, Noboru Saito

    JOURNAL OF CHEMICAL NEUROANATOMY39 ( 4 ) 272 - 288   2010年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The biochemical properties, neuroanatomical location, and function of aromatase (ARO), the enzyme that converts testosterone to 17 beta-estradiol, have been studied extensively in the adult quail brain. Conversely, very little is known about ARO in quail embryos. This study investigated the distribution of ARO in quail prosencephalon at embryonic days (E) 9, 11, and 15 by immunocytochemistry. ARO-immunoreactive cells were observed within the walls of the cerebral ventricles, the ventral striatum, medial preoptic nucleus (POM), medial part of the bed nucleus of the stria terminalis (BSTM), lateral part of the BST, and in the tuberal region. The BSTM and to a lesser extent the POM showed transient, female-biased sex-differences. In the BSTM, the number of the ARO-immunoreactive cells, the fractional area covered by ARO-immunoreactive structures, and the overall extension of ARO-immunoreactivity were greater in females at E9 and E11, but these differences largely disappeared at E15 and post-hatch day 1. The sex differences were confirmed at the transcriptional level by in situ hybridization. In the lateral part of the POM, females showed slightly more ARO-immunoreactivity than males at E11. Treatment of E9 male embryos with estradiol completely feminized ARO-immunoreactivity at E11. The origins and the functional significance of these sex differences remain unknown. (c) 2010 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jchemneu.2010.02.003

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  • TonEBP regulates hyperosmolality-induced arginine vasotocin gene expression in the chick (Gallus domesticus) 査読

    Noboru Saito, Mariko Fujii, Kanae Sugiura, Nicoletta Aste, Kiyoshi Shimada

    NEUROSCIENCE LETTERS468 ( 3 ) 334 - 338   2010年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Arginine vasotocin (AVT) is expressed mainly in the paraventircular and supraoptic nuclei of the hypothalamus in chicken. This peptide is known to act as an antidiuretic hormone and its gene expression is stimulated by hyperosmolality. However, the transcription factors that regulate the AVT gene expression induced by hyperosmolality are still unknown. In this study, we examined the role of hyper-tonicity enhancer binding protein (TonEBP) in the transcriptional regulation of AVT gene in chicken. TonEBP mRNA expression levels increased at I h after salt-loading treatment in the hypothalamus. This increase preceded that in AVT and c-fos mRNA expression. Intracerebroventricular injections of TonEBP antisense oligonucleotides, before the salt-loading treatment, prevented the increase in AVT gene expression. These results, all together, suggest that the transcription factor TonEBP may be involved in the regulation of AVT genes expression in response to a hyperosmotic environment in chicken. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2009.11.027

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  • Phospholipase Cζ mRNA expression and its potency during spermatogenesis for activation of quail oocyte as a sperm factor. 査読

    Mizushima, S, Takagi. S, Ono, T, Atsumi, Y, Tsukada, A, Saito, N, Shimada, K

    Molecular Reproduction and Development76 ( 12 ) 1200 - 1207   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/mrd.21097

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  • Identification of Two Types of Growth Hormone Receptor Mutations in Two Strains of Sex-linked Dwarf Chickens 査読

    Kenichi Tahara, Akira Tsukada, Takanobu Hanai, Kenta Okumura, Kikumi Yamada, Atsushi Murai, Rikiya Yamamoto, Makoto Maeno, Noboru Saito, Kiyoshi Shimada

    JOURNAL OF POULTRY SCIENCE46 ( 3 ) 249 - 256   2009年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN POULTRY SCIENCE ASSOC  

    Sex-linked dwarf (SLD) of White Leghorn (WL S23MA line, Okazaki station. Japan) and White Plymouth Rock (WPR 15 line, Hyogo station, Japan) strains were established from the MA line (WL) and 16 line (WPR) of a normal growing line, respectively. However, the responsible genes in the two lines of SLD chicken have not been identified. In this Study, we characterized the phenotypes and identified the responsible genes in the SLD chickens of these two strains. SLD chickens of both Strains showed low body weight, short tarsometatarsus length. and high blood growth hormone (GH) level compared with the normal growing lines. From these particular results. it was indicated that the SLID chickens might possess a defect in the growth hormone receptor (GHR). We identified two types of Mutations in the GHR gene ill each SLD chicken by Northern blot and polymerase chain reaction analyses. The S23MA line had a single base mutation ill the Splice donor Site of the exon 5/intron 5 oil the GHR gene, whereas the 15 line lacked a large part of exon 10 of the GHR R gene. which contained 27 highly conserved amino acids at the 3' end of the coding region and 3'-UTR. Furthermore. it was revealed that growth retardation was caused by reduction in food intake of the SLD chickens. These two genetically distinguishable lines of dwarf chickens would serve as all effective tool for analyzing novel GH function and GHR signal transduction in chicken.

    DOI: 10.2141/jpsa.46.249

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  • mRNA Expression of cytochrome P450 17αhydroxylase, cytochrome P450 aromatase, anti-Müllerian hormone, estrogen receptor α, and androgen receptor in developing gonads of Japanese quail. 査読

    Nakamura, K, Shibuya, K, Saito, N, Shimada, K, Ohshima, A, Hirai, T, Nunoya, T

    Journal of Poultry Science45 ( 4 ) 298 - 302   2008年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.45.298

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  • Effect of hyperosmotic stimulation on aquaporins gene expression in chick kidney 査読

    K. Sugiura, N. Aste, M. Fujii, K. Shimada, N. Saito

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY151 ( 2 ) 173 - 179   2008年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Birds can produce hyperosmotic urine, but their renal morphology differs from that of mammals. Recent studies in mammals, suggested that various aquaporins (AQPs) are present in the kidney and play crucial roles in urine production. To elucidate the role of AQPs; in the avian kidney, we first examined for the presence of AQP1, 2, 3, 4, 7 and 9 mRNAs in the chick (Gallus gallus) kidney by RT-PCR analysis. Next, we quantified variations of AQPs mRNAs levels in chick kidney after hyperosmotic stimulation (water-deprivation or salt-loading) by real-time RT-PCR analysis. Our study showed that in addition to AQP1, 2, 3, 4 and 7, chick kidney also expressed AQP9 and that hyperosmotic stimulation induced changes in AQPs; expression. In particular, water-deprivation increased AQP2 and AQP3 mRNAs levels. whereas salt-loading induced a significant increase in AQP1, AQP2 and AQP9 mRNAs levels. AQP4 and AQP7 mRNA levels were not affected by any hyperosmotic stimulation. Taken together, these results indicated that the presence of AQPs in chick kidney is similar to that in mammals, that the chick kidney has an additional AQP9 and that AQP1, 2, 3 and 9 may play a crucial but different role in water permeability in this organ. (C) 2008 Elsevier Inc. All rights reserved.

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  • Expression of P450arom, AMH and ERα mRNA in gonads of turkey, duck and goose within one week of age. 査読

    Koba, N, Ohfuji, T, Ha, Y, Mizushima, S, Tsukada, A, Saito, N, Shimada, K

    Journal of Poultry Science45 ( 3 ) 220 - 226   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Profiles of mRNA expression of FOXL2, P450arom, DMRT1, AMH. P450c17, SF1, ERα and AR, in relation to gonadal sex differentiation in duck embryo. 査読

    Koba, N, Ohfuji, T, Ha, Y, Mizushima, S, Tsukada, A, Saito, N, Shimada, K

    Journal of Poultry Science45 ( 2 ) 132 - 138   2008年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Effects of aromatase inhibitor (Fadrozole)-induced sex-reversal on gonadal differentiation and mRNA expression of P450arom, AMH and ERα in quail embryos and growth in posthaching quail. 査読

    Koba, N, Ohfuji, T, Ha, Y, Mizushima, S, Tsukada, A, Saito, N, Shimada, K

    Journal of Poultry Science45 ( 2 ) 116 - 124   2008年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Developmental enhancement of intracytoplasmic sperm injection (ICSI)-generated quail embryos by phospholipase Cζ. 査読

    Mizushima, S, Takagi, S, Ono, T, Atsumi, Y, Tsukada, A, Saito, N, Shimada, K

    Journal of Poultry Science45 ( 2 ) 152 - 158   2008年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Sex- and age-related variation in neurosteroidogenic enzyme mRNA levels during quail embryonic development 査読

    Nicoletta Aste, Yumi Watanabe, Kiyoshi Shimada, Noboru Saito

    BRAIN RESEARCH1201   15 - 22   2008年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Brain can synthesize steroids de novo from cholesterol and this biochemical feature is a conserved property of vertebrates. There is growing evidence indicating that neurosteroids might participate in sexual differentiation of the brain. Therefore, in this study we investigated the presence, the sex differences, and the development-dependent variation of mRNAs coding for key neurosteroidogenic enzymes, namely cytochrome P450 side-chain cleavage enzyme (P450scc), 3 beta-hydroxysteroid-dehydrogenase/Delta 5-Delta 4-isomerase (3 beta-HSD), cytochrome P450 17 alpha-hydroxylase/c17, 20-lyase (P450c17), and aromatase in embryonic prosencephali. Our results indicated that 3 beta-HSD mRNA levels were sexually dimorphic and developmental age-dependent. In particular, 3 beta-HSD mRNA levels were higher in females than in males at E7, whereas, this dimorphism was reversed at E9 and E15. In females, the relative levels of 3 beta-HSD mRNA were highest at E7, whereas, in males they were significantly higher at E9 and E15 than at E7 and at Ell. This sexual dimorphism was a peculiar feature of the prosencephalon, it could not be observed before gonadal sexual differentiation and it was not paralleled by a dimorphism in the brain content of progesterone. The level of mRNA coding for P450scc and for P450c17 did not show obvious developmental- or sex-related variation. Aromatase mRNA varied as a function of the embryonic age but not of the sex. These results, taken together, are suggestive of a potential role of some neurosteroidogenic enzymes in the development of quail brain and suggest that sexual differences in the hormonal environment may occur during brain development. (C) 2008 Elsevier B.V. All rights reserved.

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  • Identification of differentially expressed genes involved in the regression and development of Müllerian duct of chicken. 査読

    Ha, Y, Tsukada, A, Saito, N, Shimada, K

    International Journal of Developmental Biology52 ( 8 ) 1135 - 1141   2008年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail (Coturnix japonica) 査読

    Shusei Mizushima, Soichi Takagi, Tamao Ono, Yusuke Atsumi, Akira Tsukada, Noboru Saito, Kiyoshi Shimada

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY307A ( 11 ) 647 - 653   2007年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    Although a rise in intracellular calcium concentration of vertebrate oocytes plays a pivotal role for the initiation of fertilization or oocyte activation, no study on this subject has been reported in birds. This study was conducted to study the role of intracellular calcium in relation to fertilization in avian oocytes. First, immediately after a quail oocyte was injected with a sperm, it was treated with strontium chloride as an inducer for intracellular calcium rise at doses of 0, 2.5, 5, 7.5, 10 mM for 4 hr in the culture medium and was followed by 20-hr culture. Treatment with 5 mM of strontium chloride induced blastodermal development in 24.2% of injected eggs, although no oocytes developed without strontium treatment. Second, quail oocytes were injected with a sperm and 0.1 M calcium chloride or a sperm and saline solution, cultured without calcium for 4 hr and was followed by 20-hr culture without strontium. The calcium solution induced blastodermal development in 20.5% of the oocytes, although no oocytes developed without calcium treatment. Third, quail oocytes were injected with 1,2-bis (o-aminophenoxy) ethane-N,N,N',N-tetraacetic acid (BAPTA) as a calcium chelator, cultured with strontium (5 mM) for 4 hr followed by 20-hr culture without strontium. Only one oocyte developed after BAPTA and strontium treatment of 36 oocytes examined. Developmental stages of all the oocytes ranged from IV to VII. These results suggest that intracellular calcium rise may participate in quail oocyte activation and allow fertilization and blastodermal development.

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  • Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing the W chromosome 査読

    S. Takagi, T. Ono, A. Tsukada, Y. Atsumi, S. Mizushima, N. Saito, K. Shimada

    POULTRY SCIENCE86 ( 5 ) 937 - 943   2007年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:POULTRY SCIENCE ASSOC INC  

    Our previous study demonstrated that elongated spermatids and sperm carrying the female-specific W-chromosome of the sex-reversed domestic fowl can activate the mouse oocyte, but whether they can fertilize the avian oocyte and lead to a developing zygote remains undetermined. A single sperm isolated from the semen and testis of normal rooster and from a testis of sex-reversed hen was microinjected into a quail oocyte and cultured for 20 to 24 h. Blastoderms were fixed, cleaved, nuclei stained by 4',6'-diamidino-2-phenylindole, and developmental stages were assessed. In the normal rooster group, ejaculated and testicular sperm induced blastodermal development in 22.6 and 20%, of the quail oocytes, respectively. The developmental stages ranged from IV to VII. In the sex-reversal group, 20% of injected testicular sperm induced blastodermal development. The blastodermal stages varied from stage III to VI. Blastoderms after 4',6'-diamidino-2-phenylindole staining were assayed by PCR to identify the W chromosome of either chicken sperm or quail oocyte. The PCR assay results showed that 2 out of 9 developed blastoderms microinjected with sperm of sex-reversed hen were identified containing the female-specific W chromosome derived from sex-reversed hen. From these results, it is concluded that chicken sperm bearing the W chromosome possess fertilizing ability and can function to stimulate blastoderm development similar to that of normal chicken sperm carrying the Z chromosome.

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  • Central administration of glucagon suppresses food intake in chicks 査読

    Kazuhisa Honda, Hiroshi Kamisoyama, Noboru Saito, Yohei Kurose, Kunio Sugahara, Shin Hasegawa

    NEUROSCIENCE LETTERS416 ( 2 ) 198 - 201   2007年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Food intake in chickens is regulated in a manner similar to that in mammals. Corticotropin-releasing factor (CRF), which increases the plasma corticosterone concentration, plays an important role as a mediator of many appetite-suppressive peptides in the central nervous system in both species. Central administration of glucagon suppresses food intake in rats. However, the anorexigenic action of glucagon in chicks has not yet been identified. In the present study, we investigated the effects of central administration of glucagon on food intake in chicks. Intracerebroventricular administration of glucagon in chicks significantly suppressed food intake and significantly induced hyperglycemia. In contrast, peripheral administration of the same dose of glucagon did not influence food intake and plasma glucose concentration. These results suggest that glucagon functions in chicks as an appetite-suppressive peptide in the central nervous system. Intracerebroventricular administration of glucagon in chicks also significantly increased CRF mRNA expression and plasma corticosterone concentration, suggesting that CRF acts as a downstream molecule for a glucagon-induced appetite-suppressive pathway in chicks. It is likely that the induction of hyperglycemia by central administration of glucagon is involved in its anorexigenic action, because peripheral administration of glucose in chicks suppressed food intake. These results suggest that CRF- and/or hyperglycemia-mediated pathways are involved in the anorexigenic action of glucagon in chicks. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

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  • Z-chromosome specific primers for chicken-quail hybrid blastoderm 査読

    Soichi Takagi, Tamao Ono, Akira Tsukada, Yusuke Atsumi, Shusei Mizushima, Noboru Saito, Kiyoshi Shimada

    Journal of Poultry Science44 ( 2 ) 209 - 212   2007年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.

    DOI: 10.2141/jpsa.44.209

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  • Fertilizing ability of chicken sperm bearing W chromosome, 査読

    Takagi, S, Ono, T, Tsukada, A, Atsumi, Y, Mizushima, S, Saito, N, Shimada, K

    Poultry Science86   730 - 737   2007年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Cloning of cDNA and mRNA expression of androgen receptor in male Japanese quail (Coturnix coturnix japonica). 査読

    Mizushima, S, Saito, N, Shimada, K

    J. Poult. Sci.43 ( 2 ) 173 - 179   2006年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Effect of water deprivation on aquaporin 4 (AQP4) mRNA expression in chickens (Gallus domesticus) 査読

    N Saito, H Ikegami, K Shimada

    MOLECULAR BRAIN RESEARCH141 ( 2 ) 193 - 197   2005年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Aquaporin (AQP) 4 is a member of the AQP gene family of water-selective transport proteins. We studied the effect of water deprivation on AQP4 gene expression in chickens. The nucleotide sequence of a chicken aquaporin 4 (AQP4) cDNA that encodes a protein of 335 amino acids showed high homology to mammalian AQP4. Using Northern blotting analysis, AQP4 mRNA in chickens was observed as a band of approximately 5.5 kb in several tissues in addition to the hypothalamus, proventriculus, kidney, and breast muscle. Quantitative analysis by real-time RT-PCR analysis showed that the mRNA expression of AQP4 in the hypothalamus significantly increased after dehydration. On the other hand, the mRNA expression of AQP4 in the kidney significantly decreased after dehydration. This suggests that AQP4 may play a pivotal role in osmoregulation in the chicken brain. (c) 2005 Elsevier B.V. All rights reserved.

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  • Identification of Spermatogenic cells expressiong protamine mRNA in Japanese quail by RT-PCR 査読

    Upi Chairun Nisa, Tsukada, A, Takagi, S, Yamamoto, I, Ha, Y, Hrabia, A, Saito, N, Shimada, K

    J. Poult. Sci.42 ( 1 ) 70 - 78   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Changes in mRNA expression of MMP-2 in the Mullerian duct of chicken embryo 査読

    YJ Ha, A Tsukada, N Saito, K Shimada

    GENERAL AND COMPARATIVE ENDOCRINOLOGY139 ( 2 ) 131 - 136   2004年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Although asymmetric development of the ovary and the oviduct is a unique characteristic in birds, the mechanism of asymmetric development still remains unclear. Recently, degradation of extracellular matrix has been suggested as an important factor related to the regression of the Mullerian duct in mammals. The present study was conducted to examine a possible role of metalloproteinase-2 (MMP-2) in the regression of the right Mullerian duct in the developing chicken embryo. Morphological changes in the Mullerian ducts were studied on day 15 of incubation and mRNA expresseion of MMP-2 was studied on days 12, 15, and 18 of incubation. Morphological observation demonstrated the disappearance of basement membrane in the right Mullerian duct which undergoes the regression. RT-PCR analysis showed that MMP-2 mRNA expression of the right Mullerian duct increased on days 15 and 18 of incubation coincidently with the time of regression. In the right Mullerian duct, regression was prevented by diethylstilbestrol treatment on day 4 of incubation and a coincident decrease in MMP-2 expression was observed when compared to the control group. These results suggest that MMP-2 may be involved in the regression of the right Mullerian duct in the female embryos of the chicken. (C) 2004 Elsevier Inc. All rights reserved.

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  • Increased expression of elastolytic cysteine proteases, cathepsins S and K, in the neointima of balloon-injured rat carotid arteries 査読

    XW Cheng, M Kuzuya, T Sasaki, K Arakawa, S Kanda, D Sumi, T Koike, K Maeda, N Tamaya-Mori, GP Shi, N Saito, A Iguchi

    AMERICAN JOURNAL OF PATHOLOGY164 ( 1 ) 243 - 251   2004年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC INVESTIGATIVE PATHOLOGY, INC  

    The matrix-degrading activity of several proteases are involved in the accelerated breakdown of extracellular matrix associated with vascular remodeling during the development of atherosclerosis and vascular injury-induced neointimal formation. Previous studies have shown that the potent elastolytic cysteine proteases, cathepsins S and K, are overexpressed in atherosclerotic lesions in human and animal models. However, the role of these cathepsins in vascular remodeling remains unclear. In the present study, the expressions of cathepsin S and K and their inhibitor cystatin C were examined during arterial remodeling using a rat carotid artery balloon-injury model. The increase in both cathepsin S and K mRNA levels was observed from day I and day 3 through day 14 following the induction of balloon injury, respectively. Western blotting analysis revealed that both cathepsin S and K protein levels also increased in the carotid arteries during neointima formation, coinciding with an increase elastolytic activity assayed using Elastin-Congo red, whereas, no significant change in the expressions of cystatin C mRNA and protein was observed during follow-up periods after injury. Immunohistochemistry, Western blot, and in situ hybridization showed that the increase of cathepins S and K and the decrease of cystatin C occurred preferentially in the developing neointima. These findings suggest that cathepsin S and K may participate in the pathological arterial remodeling associated with restenosis.

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  • Profiling of mRNA expression of genes related to sex differentiation of the gonads in the chicken embryo 査読

    Yamamoto, I, Tsukada, A, Saito, N, Shimada, K

    Poultry Sci.82   1462 - 1467   2003年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Expression of Protamine mRNA in Relation to Spermatogenic Activity in Japanese Quail 査読

    Upi Chairun Nisa, Akira Tsukada, Masahiko Mori, Noboru Saito, Kiyoshi Shimada

    Journal of Poultry Science40 ( 4 ) 309 - 318   2003年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This study was conducted to elucidate relationship between testicular protamine mRNA expression and spermatogenicactivity in the quail as the following experiments. (+1) Quail were raised under continuous light condition after hatching and body weights, testis weights, spermatogenic activity and protamine mRNA levels were determined weekly between 3 and 8 weeks. (2) The other group of quail raised under continuous light condition up to 6 weeks after hatching were kept in either continuous light or reduced light-length (8-hour light, 16-hour darkness) conditions for 3 weeks. At 6 and 9 weeks old, the same parameters were measured as in the foregoing experiment. Spermatogenic activity was evaluated by Bartholomew's classification after histological examination of the testis and mRNA expression of testis protamine was analyzed by Northern blot. Between 3 and 5 weeks old, body weights, testis weights, and cloacal gland protrusion areas significantly increased and thereafter they stayed at plateau levels. Spermatogenic activity markedly increased from stage II to VI between 3 and 5 weeks old and stayed at stage VI up to 8 weeks old. Spermatids and immature sperm were firstly observed at 4 weeks old. Protamine mRNA levels were also first detected at 4 weeks old and increased at 5 weeks old. A marked expression were maintained after 6 weeks old. In contrast, short daylengths treatment after 6 weeks old significantly reduced testis weights, cloacal gland protrusion areas, spermatogenic activity, and protamine mRNA levels at 9 weeks old (P&lt
    0.05) when compared to those raised under continuous light condition. Spermatogenic activity dropped from the full activity of stage VI to elongated spermatid (IV) or spermatocyte (II-III) stage after the treatment. Protamine mRNA levels also significantly decreased in response to short daylengths. These results suggest that protamine mRNA expression is highly related to spermatogenicactivity in the quail. © 2003, Japan Poultry Science Association. All rights reserved.

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  • Effect of Endocrine Disrupters on mRNA Expression of Vitellogenin (VTG) II and Very Low Density Lipoprotein (apo VLDL) II in the Liver of Quail Embryos 査読

    Kouhei Ichikawa, Yonju Ha, Akira Tsukada, Noboru Saito, Kiyoshi Shimada

    Journal of Poultry Science40 ( 1 ) 45 - 52   2003年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The present study was conducted to assess estrogenic activity of nonylphenol (NP) and octylphenol (OP) in quail embryos by determining mRNA levels of liver vitellogenin (VTG) II and very low density lipoprotein (apoVLDL) II. The fertile eggs were treated with a single injection of either NP, OP or ethynyl estradiol (EE) at doses of 10 and 100 nmole/egg in 20μl on day 13 of incubation. In the control group the eggs were treated with the vehicle (corn oil, 20μl/egg). On day 15 of incubation the liver was collected and total RNA was extracted. Both mRNA levels were determined by RT-PCR assay and were expressed in relation to ø actin mRNA levels. No expression of VTG II mRNA was detected in the control group, whereas a marked induction of VTG II mRNA was revealed in the EE treatment. A weak but distinct expression of VTG II mRNA was evident in the NP and OP treatment groups. ApoVLDLII transcripts were detected in the control group and induced markedly by the injection of EE with higher expression in females. NP also induced considerable expression in females, whereas no transcripts were detected in males. OP also induced the transcript in females but in males OP at 10 nmole was effective. This study indicates that NP and OP possess estrogenic activity in terms of liver VTG II and apoVLDLII mRNA expression in the quail embryo, and that apoVLDLII expression in female embryo is more sensitive to estrogenic substances. © 2003, Japan Poultry Science Association. All rights reserved.

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  • cDNA Cloning and mRNA Expression of Estrogen Receptor α in Japanese Quail 査読

    Kouhei Ichikawa, Ichiro Yamamoto, Akira Tsukada, Noboru Saito, Kiyoshi Shimada

    Journal of Poultry Science40 ( 2 ) 121 - 129   2003年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The biological actions of estrogen are mediated via estrogen receptors (ER). Although chicken ER cDNA for subtypes α and β have been identified, only the β form of ER cDNA was cloned in quail. Hence, in this study the cDNA of ERα subtype was cloned and levels of mRNA expression were studied in different tissues in quail. Quail ERα cDNA containing the sequence containing the open reading frame (1770bp) encodes the predicted 589 amino acid residues and had high homology to chicken ERa (98.8%). Both RT-PCR and northern blot analyses indicate that relatively higher expression was observed in the liver, ovary, oviduct and testes. Relatively lower expression was detected in adrenal, cerebrum, hypothalamus, gizzard, heart, kidney and intestine by RT-PCR analysis. Conversely, low levels of expression of quail ERβ mRNA was detected in all the examined tissues. The results indicate that ERa subtype plays a predominant role for estrogenic actions in reproduction in quail. © 2003, Japan Poultry Science Association. All rights reserved.

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  • cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo 査読

    Yamamoto, I, A Tsukada, N Saito, K Shimada

    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION1579 ( 2-3 ) 185 - 188   2002年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo. (C) 2002 Elsevier Science B.V All rights reserved.

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  • Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β, and Cytochrome P450 Aromatase in the Gonad of Chicken Embryos 査読

    Masanori Sakimura, Akira Tsukada, Noboru Saito, Kiyoshi Shimada, Makoto Usami, Yasuo Ohno, Shozo Hanzawa

    Journal of Poultry Science39 ( 4 ) 302 - 309   2002年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Effects of estradiol-17β (E2) and p-nonylphenol (NP) on mRNA expression of estrogen receptor α (ERα), estrogen receptor β (ERβ) and cytochrome P450 aromatase (P450arom) were examined in the early stage of chicken embryonic gonads. Fertilized eggs were treated with either E2 (1.0mg/egg) or NP (0.01, O.1mg/egg) immediately before incubation (day 0 of incubation) and were incubated at 37.5°C. In the control group the eggs were treated with the vehicle (10% propanediol, PD, 50μl). On day 10 of incubation the gonad was collected and mRNA expression was determined for semi-quantitative assay by the reverse transcription-polymerase chain reaction (RT-PCR). In the control group, ERα and P450arom mRNA showed higher expression in females than in males while ERβ mRNA showed no sexual difference. In the E2 treated group, the expression of ERα and P450arom mRNA markedly increased in males (about 4- and 16-fold, respectively). NP increased mRNA expression of ERα and P450 arom (about 2-fold each) in males. ERβ mRNA expression did not apparently change after the E2 or NP treatment in both sexes. These results suggest that NP induces some female-specific genes in the male gonads during the critical period of sex determination in chicken embryos. © 2002, Japan Poultry Science Association. All rights reserved.

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  • Supression effect of p-nonylphenol on male-specific mRNA expression in the embryonic gonad of chickens 査読

    Sakimura, M, Usami, M, Hanzawa, S, Tsukada, A, Saito, N, Ohno, Y, Shimada, K

    J. Poult. Sci.39 ( 2 ) 91 - 99   2002年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.39.91

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  • Molecular cloning of chicken vasoactive intestinal polypeptide receptor complementary DNA, tissue distribution and chromosomal localization 査読

    N Kansaku, K Shimada, T Ohkubo, N Saito, T Suzuki, Y Matsuda, D Zadworny

    BIOLOGY OF REPRODUCTION64 ( 5 ) 1575 - 1581   2001年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC STUDY REPRODUCTION  

    Chicken vasoactive intestinal polypeptide receptor (VIPR) cDNA was cloned by the reverse transcription-polymerase chain reaction method using primers designed on the basis of other species of VIPR cDNA. The cDNA obtained was sequenced by the dideoxy-mediated chain-termination method, Of the 2227 nucleotides that were sequenced, 84, 855, and 1338 bases represent the 5 ' -untranslated region (UTR), the 3 ' -UTR, and the open reading frame that predicts a peptide of 446 amino acids. The cDNA of the chicken VIPR shows 65% and 60% homologies to human cDNA of VIP1 and VIP2 receptors, respectively. The clone had the expected similarity to highly conserved features of the other G protein-coupled receptors (GPCRs) such as six cysteine residues that are functionally important in the VIPR subfamily. In addition, the seven potential membrane-spanning domains characteristic of the family B group III GPCR superfamily and highly conserved motif within the third cellular loop between transmembrane regions 5 and 6. Northern blot hybridization analysis in this study indicated mRNA expression of VIPRs in the various tissues of the chicken. Strong signal was detected in the brain and anterior pituitary gland. High levels of VIPR mRNA in the brain was consistent with VIP-binding experiments and with the function of VIP in the brain as a neuroendocrine factor or neurotransmitter. Expression of VIPR was detected in the anterior pituitary gland of chick embryos, The expression of VIPR mRNA in the chick anterior pituitary gland may indicate a regulatory function of VIP on prolactin (PRL) production or PRL cell proliferation during embryogenesis. Chicken VIPR shows high homology with mammalian type I VIPR but, in some part, possesses similarity of amino acid sequence. Expression of VIPR in various tissues supports diverse functions for VIP in the chicken.

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  • Effect of AVT antisense oligodeoxynucleotides on AVT release induced by hypertonic stimulation in chicks 査読

    N Saito, T Sasaki, M Furuse, K Arakawa, K Shimada

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY128 ( 1 ) 147 - 153   2001年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    In birds, arginine vasotocin (AVT) and mesotocin (MT) are the neurohypophyseal hormones. AVT is known to be an avian antidiuretic hormone and is released from the neurohypophysis by dehydration or hyperosmotic stimulation. The purpose of this study was to determine whether the mechanism of AVT synthesis is related to the mechanism of hormone release from the neurohypophysis. Four-day-old chicks received an AVT antisense oligodeoxynucleotide (ODN) injection into the cerebral ventricle (icv). Following antisense administration, the chicks received hypertonic saline stimulation. Plasma levels of AVT and MT were measured by radioimmunoassays. In control birds, a hypertonic saline injection resulted in the increase of plasma AVT level. The administration of a high dose (50 mug) of antisense ODN inhibited the increase of plasma AVT level induced by the hypertonic saline stimulation. Plasma levels of MT did not change with the administration of hypertonic saline or antisense ODN. These results suggest that the mechanisms that regulate the secretion of AVT from the neurohypophysis may be coupled to the mechanisms that regulate the synthesis of AVT. (C) 2001 Published by Elsevier Science Inc.

    DOI: 10.1016/S1095-6433(00)00290-7

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  • Effect of estradiol and nonylphenol on mRNA levels of vitellogeninII in the liver of chicken embryos. 査読

    Sakimura M, Hanazawa S, Tsukada A, Yamamoto I, Saito N, Usami M, Ohno Y, Shimada K

    J. Poult. Sci.38   250 - 257   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.38.250

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  • Changes in calcitonin receptor binding in the shell gland of laying hens (Gallus domesticus) during the oviposition cycle. 査読

    Ieda, T, Takahashi, T, Saito, N, Yasuoka, T, Kawashima, M, Izumi, T, Shimada, K

    J. Poult. Sci.38 ( 3 ) 203 - 212   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.38.203

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  • Intracerebroventricular injeciton of fusaric acid attenuates the anorexia by glucagon-like peptide-1 in the neonetal chick. 査読

    Bungo, T, Kawakami, S. I, Ohushi, A, Sasaihara, K, Saito, N, Sugahara, K, Hasegawa, S, Denbow, D. M, Furuse, M

    Pharmacology, Biochemistry and Behavior 70, 251-255.70 ( 2/3 ) 251 - 255   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S0091-3057(01)00613-X

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  • Regulation of opioid peptides on the release of arginine vasotocin in the hen 査読

    T Sasaki, K Shimada, N Saito

    JOURNAL OF EXPERIMENTAL ZOOLOGY286 ( 5 ) 481 - 486   2000年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    Arginine vasotocin (AVT), an avian neurohypophysial hormone, is released during osmotic stimulation and oviposition. In the present study, the role of opioid peptides on AVT release was studied by examining the effects of an opioid agonist and antagonist on osmotic- and oviposition-induced secretion of AVT. The administration of hypertonic saline (1.5 M NaCl) induced an increase in the plasma levels of AVT. The simultaneous administration of morphine, an opioid receptor agonist, inhibited the osmotically induced increase in plasma levels of AVT in a dose-dependent manner. On the other hand, the co-administration of morphine with naloxone, an opioid receptor antagonist, attenuated the inhibitory effect of morphine. Moreover, injection of naloxone alone enhanced the osmotically induced increase in plasma levels of AVT. However, the administration of morphine did not inhibit the oviposition-induced increase in plasma levels of AVT. These results suggest that osmotic-induced release of AVT may be under opioid regulation, while oviposition-induced release of AVT may be controlled by a different mechanism. (C) 2000 Wiley-Liss, Inc.

    DOI: 10.1002/(SICI)1097-010X(20000401)286:5<481::AID-JEZ5>3.0.CO;2-J

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  • Changes in parathyroid hormone-related peptide receptor binding in the shell gland of laying hens (Gallus domesticus) during the oviposition cycle 査読

    T Ieda, T Takahashi, N Saito, T Yasuoka, M Kawashima, K Shimada

    GENERAL AND COMPARATIVE ENDOCRINOLOGY117 ( 2 ) 182 - 188   2000年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC  

    In this study, parathyroid hormone (PTH) receptor binding was examined in the membrane fraction of the endometrium of the shell gland of laying and nonlaying hens by the use of I-125-PTH-related peptide (PTHrP) binding assays. Specific I-125-PTHrP binding was found in the endometrium of the shell gland and increased during the first 1 h of incubation and then reached a plateau. Specific I-125-PTHrP binding increased in line with an increase in protein concentration from 10 to 80 mu g The membrane fraction had specific binding to I-125-PTHrP. The Scatchard plot analysis revealed a linear profile that indicated the presence of one type of receptor. The equilibrium dissociation constant (Kd) and the maximum binding capacity (B-max) in the shell gland decreased 19 h before oviposition in egg-laying hens and remained low for about 20 h during the period of calcification. In contrast, the (K)d and the B-max in nonlaying hens were constant throughout a 24-h period. These results suggest that PTH acts on the endometrium of the shell gland during the eggshell calcification. (C) rare Academic Press.

    DOI: 10.1006/gcen.1999.7395

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  • Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken 査読

    FI Tan, SJ Lolait, MJ Brownstein, N Saito, MacLeod, V, DA Baeyens, PR Mayeux, SM Jones, LE Cornett

    BIOLOGY OF REPRODUCTION62 ( 1 ) 8 - 15   2000年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC STUDY REPRODUCTION  

    In chickens, oviposition is correlated with increased plasma levels of the neurohypophysial hormone vasotocin, and vasotocin stimulates contraction of uterine strips in vitro. A gene encoding a vasotocin receptor subtype that we have designated the VT1 receptor was cloned from the domestic chicken. The open reading frame encodes a 370-amino acid polypeptide that displays seven segments of hydrophobic amino acids, typical of guanine nucleotide-protein-coupled receptors. Other structural features of the VT1 receptor include two potential N-linked glycosylation sites in the extracellular N-terminal region, a conserved aspartic acid in transmembrane domain 2 that is found in nearly all guanine nucleotide-protein-coupled receptors, and two potential protein kinase C phosphorylation sites in the third intracellular loop and C-terminal tail. Expressed VT1 receptors in COS7 cells bind neurohypophysial hormones with the following rank order of potency: vasotocin congruent to vasopressin &gt; oxytocin congruent to mesotocin &gt; isotocin. In addition, the expressed VT1 receptor mediates vasotocin-induced phosphatidylinositol turnover and Ca2+ mobilization. In the chicken, expression of VT1 receptor gene transcripts is limited to the shell gland (uterus) and the brain. Thus, the VT1 receptor that we have cloned may mediate contractions of the shell gland during oviposition and activate reproductive behaviors known to be stimulated by vasotocin in lower vertebrates.

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  • Sex differentiation and mRNA expression of P450c17, P450arom and AMH in gonads of the chicken 査読

    H Nishikimi, N Kansaku, N Saito, M Usami, Y Ohno, K Shimada

    MOLECULAR REPRODUCTION AND DEVELOPMENT55 ( 1 ) 20 - 30   2000年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017 alpha hydroxylase (P450c17), P450 aromatase (P450arom) and anti-Mullerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads at days 4-8 of incubation were assessed by in situ hybridization analysis using digoxigenin labeling method. The in situ hybridization data were analyzed by relative expression of specific hybridizable signals of each mRNA corrected by the non-specific background by employing an image analyzer. P450c17 mRNA expression increased rapidly at day 6 of incubation in the male but decreased thereafter. In contrast to the transient expression in the male, the expression was gradually increased in the female. P450arom mRNA was not expressed in the male but was detectable in the female as early as day 6 and increased subsequently with days of incubation. AMH mRNA was expressed as early as day 5 of incubation followed by a sharp increase on day 6, which was maintained in the male thereafter In contrast, the female showed very little expression. The injection of Fadrozole caused no effect on P450c17 mRNA expression, while it suppressed P450arom mRNA expression but increased AMH mRNA expression in the female. In contrast, the injection of estradiol induced P450arom mRNA expression significantly but suppressed AMH mRNA expression in the mate. These results indicate that expression of P450arom and AMH is sexually dimorphic and is reciprocally regulated during early ontogenic life in chicken gonads. (C) 2000 Wiley-Liss, Inc.

    DOI: 10.1002/(SICI)1098-2795(200001)55:1<20::AID-MRD4>3.0.CO;2-E

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  • The effects of highly selective opioid receptor antagaonists on the release of AVT induced by hyperosmotic stimulation and angiotensin II injection. 査読

    Sasaki, T, Arakawa, K, Shimada, K, Saito, N

    General and Comparative Endocrinology118   365 - 372   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1006/gcen.2000.7470

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  • Comparative effect of isolated soybean protein and casein on body fat deposition in chicks. 査読

    Honda, K, Kamiyosayama, H, Kato, H, Furuya, N, Ishiwata, H, Motoki, T, Saito, N, Hasegawa, S

    Japanese Poultry Science37 ( 6 ) 365 - 371   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.37.365

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  • Expression of messenger ribonucleic acids of luteinizing hormone and follicule-stimulating hormone receptors in the embryonic and posthatch gonads of the chicken. 査読

    Mao, X, Zhang, C, Saito, N, Shimada, K

    Japanese Poultry Science37   212 - 220   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.37.212

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  • Gene expression of arginine vasotocin in ovarian and uterine tissues of the chicken 査読

    N Saito, R Grossmann

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES12 ( 5 ) 695 - 701   1999年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC  

    The hypothalamus is the classic site of synthesis of arginine vasotocin as neurohypophyseal hormone in the chicken. However, high concentrations of arginine vasotocin were also measured in ovarian tissues by radioimmunoassay. At first. we observed specific positive signal of mRNA encoding AVT in the hypothalamus by Northern hybridization. However, we could not find any specific bands in ovarian and uterine tissues. For evidence of transcription of the arginine vasotocin gene in gonadal tissues of the chicken, this study has applied the polymerase chain reaction as a highly sensitive assay. The hypothalamus, the four largest preovulatory ovarian follicles and the shell gland (uterus) were collected at 4 h and 20 h before oviposition. The ovarian follicular tissues were separated into granulosa theca interns and theca externa layers. The uterine tissues were separated into myometrium and endometrium The extracted mRNA was converted to cDNA by reverse-transcriptase using oligo-d(T)(15) primer. Then, the cDNA was amplified by Vent polymerase and arginine vasotocin specific primers. The amplification reaction was incubated by 30 cycles successively, 95 degrees C, 55 degrees C and 72 degrees C earth for 1 min. The comparisons of the mRNA levels encoding arginine vasotocin between the tissues were determined by semi-quantification methods. After amplification of the cDNA, the PCR products were detected in hypothalamus, ovarian tissues and uterine tissues. The results of semi-quantification showed that the levels of arginine vasotocin mRNA in ovarian iud uterine tissues were about from 1/50 to 1/1000 when compared to that in the hypothalamus. The very low levels of mRNA encoding arginine vasotocin in ovarian and uterine tissues probably led us to conclude that arginine vasotocin may play a role of local mediate acting autocrine and/or paracrine.

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  • Effects of naloxone on neurohypophyseal peptide release by hypertonic stimulation in chicks 査読

    N Saito, M Furuse, T Sasaki, K Arakawa, K Shimada

    GENERAL AND COMPARATIVE ENDOCRINOLOGY115 ( 2 ) 228 - 235   1999年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC  

    The effects of opioid peptides on the osmotic release of neurohypophyseal hormones, arginine vasotocin (AVT) and mesotocin (MT), were determined in 2-day-and chicks. Experiment 1 examined the effect of a variety of doses of naloxone, an opioid antagonist, on chicks administered isotonic or hypertonic solution. Plasma osmolality in chicks administered hypertonic solution was significantly higher than that in groups administered isotonic solution. None of the doses of naloxone affected plasma osmolality in response to isotonic and hypertonic solution. Plasma levels of AVT increased in hypertonic solution and this response was further enhanced by naloxone injection as the doses increased. The hypertonic solution alone did not affect plasma levels of MT, but additional treatment with naloxone slightly increased plasma levels of MT. Experiment 2 examined the effect of DAMGO ([D-Ala(2),N-Me-Phe(4), Gly-ol]-enkephalin), a specific mu receptor agonist. Relatively high plasma osmolality caused by hypertonic solution was not affected by additional treatment with DAMGO. Plasma levels of AVT in response to hypertonic solution and to additional treatment with naloxone were reduced by higher doses of DAMGO. Experiment 3 examined the effect of naloxone on chicks administered different concentrations of NaCl. Administration of hypertonic solution resulted in an increase in plasma osmolality and plasma levels of AVT. Naloxone administration enhanced the increase in plasma AVT levels in response to hypertonic solution. Experiment 4 examined the effect of naloxone on different kinds of hypertonic solution, 0.15 M NaCl, 1.5 M NaCl, 2.55 M urea, and 1.95 M sucrose. The increases in plasma osmolality resulting from the administration of the urea and sucrose solutions were the same as those in the chicks injected with 1.5 M NaCl. In sucrose-treated chicks, plasma levels of AVT increased in chicks administered naloxone but not in chicks injected with normal saline. In contrast, no;significant changes in plasma levels of AVT were observed in urea treatment with or without naloxone. In Experiments 3 and 4, plasma levels of MT after administration of hypertonic solutions did not change. However, naloxone administration enhanced plasma levels of MT in osmotically stimulated chicks. The results of the present study suggest that opioid peptides attenuate the increase in plasma AVT and MT in hypertonic states. (C) 1999 Academic Press.

    DOI: 10.1006/gcen.1999.7302

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  • Effect of low calcium diet on messenger ribonucleic acid levels of calbindin-D28K of intestine and shell gland in laying hens in relation to egg shell quality. 査読

    Ieda, T, Saito, N, Shimada, K

    Japanese Poultry Science 36, 295-303.36 ( 5 ) 295 - 303   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.36.295

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  • Intraventricular injection of glucagon-like peptide-1 inhibits water intake of the neonatal chick. 査読

    Furuse, M, Matsumoto, M, Pinotoan, R, Saito, N, Sugahara, K, Hasegawa, S

    Japanese Poultry Science36 ( 4 ) 229 - 235   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.36.229

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  • Effects of naloxone on the anorexic action by glucagon-like peptide-1 (7-36) in the neonatal chick. 査読

    Bungo, T, Shimojo, M, Masuda, Y, Saito, N, Sugahara, K, Hasegawa, S, Furuse, M

    Japanese Poultry Science36 ( 2 ) 109 - 115   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.36.109

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  • Influence of intracerebroventricular administration of morphine and kyotrophin on food intake of the neonatal chick. 査読

    Bungo, T, Ando, R, Ao, R, Shimojo, M, Masuda, Y, Choi ,Y.-H, Saito, N, Furuse, M

    Japanese Poultry Science36 ( 5 ) 319 - 324   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.36.319

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  • Control gastrin inhibits leeding behavior and food passage in neonatal chicks 査読

    Furuse, M, Ao, R, Bungo, T, Ando, R, Shimojo, M, Masuda, Y, Saito, N

    Life Science65 ( 3 ) 305 - 311   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S0024-3205(99)00249-0

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  • Effects of substitution of N-terminal amino acid of glucagon-like peptide-1 (7-36) amide on food intake of the neonatal chick 査読

    Furuse, M, Ao, R, Bungo, T, Ando, R, Shimojo, M, Masuda, Y, Saito, N

    Life Sciences25   PL295   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Intracerebroventricularly administration of glucagon-like peptide-1 induces sleep-like behavior in the neonatal chick. 査読

    Bungo, T, Shimojo, M, Masuda, Y, Saito, N, Sugahara, K, Hasegawa, S, Furuse, M

    Japanese Poultry Science36 ( 6 ) 377 - 381   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.36.377

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  • Expression of messenger ribonucleic acids of luteinizing hormone receptor, P450 17a-hydroxylase and P450 aromatase in isolated granulosa, theca interna and theca externa layers of chicken ovarian follicles during follicular growth. 査読

    Kansaku, N, Tsuji, Y, Saito, N, Shimada, K

    Animal Science Journal70   12 - 17   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2508/chikusan.70.12

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  • Refeeding increases hepatic insulin-like growth factor-I (IGF-I) gene expression and plasma IGF-I concentration in fasted chicks 査読

    K Kita, K Hangsanet, T Shibata, MA Conlon, T Sasaki, N Saito, J Okumura

    BRITISH POULTRY SCIENCE39 ( 5 ) 679 - 682   1998年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CARFAX PUBLISHING  

    1. We examined the influence of refeeding after 2 d of fasting on plasma insulin-like growth factor-I (IGF-I) concentration and hepatic IGF-I gene expression in chickens at 6 weeks of age.
    2. Hepatic IGF-I mRNA was measured by ribonuclease protection assay and plasma IGF-I concentration was determined by radioimmunoassay.
    3. Plasma IGF-I concentration decreased following fasting, increased to the level of fed controls after 2 h of refeeding but then fell back to the level of fasted chickens after 6 h of refeeding.
    4. Fasting reduced hepatic IGF-I mRNA concentrations to less than half of those in the fed controls. Refeeding increased IGF-I mRNA sharply at 2 h after refeeding, but by 6 h after refeeding they had taller back again to levels significantly lower than at 2 h.
    5. A significant correlation between plasma IGF-I concentration and hepatic IGF-I gene expression was found, suggesting that when chicks are refed after 2 d of fasting, the short-term increase in plasma IGF-I concentration may be partly regulated by the alteration in hepatic IGF-I mRNA.

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  • Refeeding increases hepatic insulin-like growth factor-I (IGF-I) gene expression and plasma IGF-I concentration in fasted chicks 査読

    K Kita, K Hangsanet, T Shibata, MA Conlon, T Sasaki, N Saito, J Okumura

    BRITISH POULTRY SCIENCE39 ( 5 ) 679 - 682   1998年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CARFAX PUBLISHING  

    1. We examined the influence of refeeding after 2 d of fasting on plasma insulin-like growth factor-I (IGF-I) concentration and hepatic IGF-I gene expression in chickens at 6 weeks of age.
    2. Hepatic IGF-I mRNA was measured by ribonuclease protection assay and plasma IGF-I concentration was determined by radioimmunoassay.
    3. Plasma IGF-I concentration decreased following fasting, increased to the level of fed controls after 2 h of refeeding but then fell back to the level of fasted chickens after 6 h of refeeding.
    4. Fasting reduced hepatic IGF-I mRNA concentrations to less than half of those in the fed controls. Refeeding increased IGF-I mRNA sharply at 2 h after refeeding, but by 6 h after refeeding they had taller back again to levels significantly lower than at 2 h.
    5. A significant correlation between plasma IGF-I concentration and hepatic IGF-I gene expression was found, suggesting that when chicks are refed after 2 d of fasting, the short-term increase in plasma IGF-I concentration may be partly regulated by the alteration in hepatic IGF-I mRNA.

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  • Effects of short-term dehydration on plasma osmolality, levels of arginine vasotocin and its hypothalamic gene expression in the laying hen 査読

    N Saito, R Grossmann

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY121 ( 3 ) 235 - 239   1998年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    The neurohypophysial hormone, arginine vasotocin (AVT), plays an important role in the osmoregulation of birds. After a prolonged period of water deprivation, plasma osmolality and plasma concentration of AVT are elevated. In this study, the effects of short term dehydration were examined in laying hens by measuring plasma osmolality, plasma levels of potassium, sodium and AVT and hypothalamic concentrations of mRNA encoding AVT during 8 h of water deprivation. Plasma osmolality increased significantly after at 6 h of water deprivation. Plasma sodium levels, however, did not change. Plasma potassium concentrations gradually decreased during dehydration. Plasma AVT levels and hypothalamic AVT mRNA levels increased significantly after 8 h. The results of this study demonstrate that depriving chickens of water results first in an increase in plasma osmolality followed by increases in AVT levels in plasma and AVT mRNA levels in the hypothalamus. The data indicate that the synthesis of AVT in the magnocellular neurons in the hypothalamus is activated soon after the animals are deprived of water. This indicates that both de novo synthesized AVT as well as AVT stored in the neurohypophysis are available to meet the increasing demands for the hormone during osmotic stress. (C) 1998 Elsevier Science Inc. All rights reserved.

    DOI: 10.1016/S1095-6433(98)10123-X

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  • Effects of various N-terminal fragments of glucagon-like peptide-1(7-36) on food intake in the neonatal chick 査読

    M Furuse, T Bungo, M Shimojo, Y Masuda, N Saito, S Hasegawa, K Sugahara

    BRAIN RESEARCH807 ( 1-2 ) 214 - 217   1998年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Recently, the suppressive effect on food intake by the central administration of glucagon-like peptide-1 (GLP-1) has been confirmed in both rats and chicks, The importance of the N-terminal amino acid, histidine, for the bioactivity of GLP-1(7-36) in the central nervous system was suggested, though the role for C-terminal amino acids in the central nervous system has not been reported, The present study was done to elucidate the central effect of N-terminal fragments of GLP-1(7-36) on food intake of the neonatal chick. Intracerebroventricular (i.c.v.) administration of mammalian GLP-1(7-36) inhibited food intake of chicks, hut the fragments of GLP-1(7-16) and GLP-1(7-26) did not show the suppressive effect on food intake. Furthermore, the extended fragments, GLP-1(7-30) and GLP-1(7-33), also had no effects on food intake. It is concluded that C-terminal amino acids of GLP-1(7-36) have an important role for the bioactivity in the central nervous system with special reference to feeding behavior. (C) 1998 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0006-8993(98)00798-7

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  • Changes of AVT levels in plasma, neurohypophysis and hypothalamus in relation to oviposition in the laying hen 査読

    T Sasaki, K Shimada, N Saito

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY121 ( 2 ) 149 - 153   1998年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Arginine vasotocin (AVT) is a neurohypophysial hormone involved in the reproductive function in avian species. We measured AVT concentrations in the neurohypophysis and plasma in relation to indomethacin blocked oviposition and to prostaglandin (PG) E-2-induced premature oviposition in the hen. In addition, AVT concentration in the hypothalamus was measured in relation to spontaneous oviposition. In the control group the concentration of AVT decreased in the neurohypophysis and increased in plasma at oviposition. In hens that were administrated indomethacin, oviposition was delayed for several hours and no changes in the levels of AVT in neurohypophysis and plasma were observed at the predicted time of oviposition. Premature oviposition was induced within 5 min after the administration of PGE(2).. The levels of AVT decreased in the neurohypophysis and increased in plasma at the PGE(2)-injected premature oviposition. The increases of AVT levels in paraventricular and supraoptic nuclei were observed 2 and 5 h after spontaneous oviposition. The results indicate that oviposition causes an increase in the release of AVT from neurohypophysis and suggest that the synthesis of hypothalamic AVT may be stimulated by oviposition. (C) 1998 Elsevier Science Inc. All rights reserved.

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  • Role of retinoic acid in regulation of mRNA expression of CaBP-D28k in the cerebellum of the chicken 査読

    K Matsumoto, T Ieda, N Saito, T Ono, K Shimada

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY120 ( 2 ) 237 - 242   1998年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    In contrast to vitamin D-3-dependent gene expression of calbindin (CaBP-D-28k) in intestine and kidney, the cerebellar mRNA expression seems independent of vitamin D-3. The present study was conducted to elucidate correlation of mRNA expression of CaBP-D-28k and vitamin D-3 receptor (VDR) in the kidney and cerebellum of the developing chick by Northern blot analysis, localization of CaBP-D28k mRNA within the cerebellum by in situ hybridization, and effect of retinoic acid in ovo on CaBP-D-28k mRNA levels. CaBP-D-28k mRNA levels were low in the cerebellum until embryonic day 16 (E16) but markedly increased on E18 and reached plateau levels on E20. VDR mRNA levels were low until E16 and significantly increased on E18 bur decreased on E20 and remained low on 1 and 7 days after hatching. In the mesonephros, CaBP-D-28k mRNA levels were high until E16 but abruptly decreased on E18, while VDR mRNA levels remained relatively constant throughout the examined period between E10 and 20. In situ hybridization analysis clearly demonstrated CaBP-D-28k mRNA signals within the Purkinje cells of the cerebellum in the embryo on E12-E18. Although 1,25 dihydroxyvitamin D-3 [1,25(OH)(2)D-3, 3 x 10(-10) M] injected in ovo on E15 increased CaBP-D-28k mRNA levels in the mesonephros on E10 but had no effect on those in the cerebellum. On the contrary, in ovo injection of retinoic acid (10(-10) and 10(-8) M) caused no effect on CaBP-D-28k mRNA levels in the mesonephros but significantly increased those in the cerebellum. The results indicate a regulatory role of retinoic acid on mRNA expression of CaBP-D-28k in the cerebellar Purkinje cells of the chicken embryo. (C) 1998 Elsevier Science Inc. All rights reserved.

    DOI: 10.1016/S1095-6433(98)00022-1

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  • Identification of sperm-bearing female-specific chromosome in the sex-reversed chicken 査読

    Abinawanto, C Zhang, N Saito, Y Matsuda, K Shimada

    JOURNAL OF EXPERIMENTAL ZOOLOGY280 ( 1 ) 65 - 72   1998年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    Sexual differentiation in the female chick embryo was phenotypically reversed to the male sex by injection of an aromatase inhibitor (Fadrozole, 0.1 mg/egg) into the embryo at day 5 of incubation. The birds were raised to 10 months of age, and the morphology of the gonads of sex-reversed hens were examined by the light-microscopic morphology, and the presence of the W chromosome gene was determined by fluorescence in situ hybridization (FISH) technique and used for PCR analysis of a single isolated sperm. The sex-reversed hens possessed two testes with a fully developed oviduct on the left side. The testes contained essentially the same cellular components as those of normal testes, although sperm counts were low. FISH analysis revealed numerous spermatids and several sperm bearing W-chromosomes, indicating that the second meiosis occurred normally but that the transformation from the spermatid to the spermatozoon is partially impaired. PCR analysis using the DNA of a single sperm also indicates that sperm-carrying the W chromosome were produced. (C) 1998 Wiley-Liss, Inc.

    DOI: 10.1002/(SICI)1097-010X(19980101)280:1<65::AID-JEZ8>3.0.CO;2-F

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  • Relationship of insulin-like growth factor-I to muscle protein synthesis in fasted chicks. 査読

    Kita, K, Shibata, T, Aman Yaman, M, Conlon, M. A, Sasaki, T, Saito, N, Okumura, J

    Japanese Poultry Science35 ( 5 ) 263 - 270   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Effects of protein kinase A inhibitor (H-89) on VIP- a GRF-iuced release a mRNA expression of prolactin a growth hormone in the chicken pituitary gla 査読

    Kansaku, N, Shimada, K, Saito, N, Hidaka, H

    Comparative Biochemistry a Physiology119C   89 - 95   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S0742-8413(97)00195-3

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  • Influence of intracerebroventricular administration of exendin (9-39) on food intake of the newly-hatched chick. 査読

    Furuse, M, Bungo, T, Shimojo, M, Masuda, Y, Saito, N, Hasegawa, S, Sugahara, K

    Japanese Poultry Science35 ( 6 ) 376 - 380   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Effects of aminoglutethimide on levels of messenger ribonucleic acid of CaBP-D28k and of vitamin D3 receptor in the shell gland of laying hen. 査読

    Ieda, T, Saito, N, Shimada K

    Japanese Poultry Science35 ( 6 ) 346 - 355   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • The central corticotropin-releasing factor and glucagon-like peptide-1 in food intake of the neonatal chick 査読

    Mitsuhiro Furuse, Megumi Matsumoto, Noboru Saito, Kunio Sugahara, Shin Hasegawa

    European Journal of Pharmacology339 ( 2-3 ) 211 - 214   1997年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Recently, we have reported that central administration of glucagon-like peptide-1 (GLP-1) strongly decreases food intake of chicks. The aim of this study was to elucidate whether suppressed food intake induced by the central injection of GLP-1 is mediated by activation of the hypothalamic-pituitary-adrenal axis. First, the effects of central administration of corticotropin-releasing factor (CRF) were investigated. Birds (2-day-old) were food-deprived for 3 h and then CRF or saline was injected intracerebroventricular (i.c.v.). CRF strongly inhibited food intake. Thereafter, effects of central CRF or GLP-1 on plasma corticosterone concentration were examined. CRF significantly stimulated corticostersne release, but GLP-1 did not alter plasma corticosterone concentration. These results suggest that CRF is a potent inhibitor of food intake in the chick, but the suppression of food intake induced by central GLP-1 may not be involved in the activation of hypothalamic-pituitary-adrenal axis.

    DOI: 10.1016/S0014-2999(97)01391-5

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  • Effect of oestradiol replacement in ovariectomized chickens on pituaitary LH concentrations and concentrations of mRNAs encoding LHβ and αsubunits. 査読

    Terada, O, Shimada, K, Saito, N

    J. Reprod. Fert.111 ( 1 ) 59 - 64   1997年9月

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  • Expression of messenger ribonucleic acids of luteinizing hormone and follicle-stimulating hormone receptors in granulosa and theca layers of chicken preovulatory follicles 査読

    CQ Zhang, K Shimada, N Saito, N Kansaku

    GENERAL AND COMPARATIVE ENDOCRINOLOGY105 ( 3 ) 402 - 409   1997年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Expression of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) mRNAs was demonstrated in the granulosa and theca layers of the large preovulatory follicles of the chicken ovary by Northern hybridization and reverse transcription-polymerase chain reaction (RT-PCR). Northern hybridization results showed multiple LHR and FSHR mRNA transcripts and the predominant species were 2.3 and 2.5 kb, respectively. The highest abundance of LHR mRNA was found in the granulosa layer of the largest follicle (F-1 follicle), while the abundance remained low in the granulosa layers of the third (F-3) and fifth largest (F-1) follicles. FSHR mRNA abundance was the highest in the granulosa layer of F-5 follicle, but decreased in the granulosa layers of F-3 and F-1 follicles. In the theca layers of all three follicles examined LHR and FSHR mRNAs were extremely low. These results were confirmed by RT-PCR experiment which involved coamplification of LHR or FSHR mRNA and beta-actin mRNA as the internal control. The LHR PCR product was sequenced and indicated 92.2% homology with the corresponding region of the quail LHR cDNA. This study indicated that the marked increase in expression of LHR mRNA in granulosa layer of the F-1 follicle might be important for LHR protein synthesis and succeeding bonus progesterone production in F-1 follicle destined to ovulation. However, higher expression of FSHR mRNA in the granulosa layer of the less mature follicles may be involved in the differentiation and maturation of granulosa cells in these follicles. (C) 1997 Academic Press.

    DOI: 10.1006/gcen.1996.6843

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  • Possible AVT involvement in regulation of body water balance dureing the inusbation period in the chabohen 査読

    Shimada, K, Saito, N

    Japanese Poultry Science34 ( 3 ) 169 - 174   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • The central corticotropin-releasing a glucagon-like peptide-1 in food intake of the neonatal chick 査読

    Furuse, M, Matsumoto, M, Saito, N, Sugahara, K, Hasegawa, S

    European Journal of Pharmacology339 ( 2/3 ) 211 - 214   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Effects of aromatase inhibitor on sex differentiation a levels of P450c17 a P450 arom mRNA of gonads in broiler embryos a broiler chickens 査読

    Abinawanto, Shimada, K, Saito, N, Sugishima, T, Shiokawa, E

    Japanese Poultry Science34 ( 5 ) 318 - 328   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Sex-reversal effects of non-steroidal aromatase inhibitor on aromatase (P450arom) mRNA expression in adult chicken gonads 査読

    Abinawanto, Shimada, K, Saito, N

    Japanese Poultry Science34 ( 3 ) 158 - 168   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Expression of P450<sub>17α</sub> hydroxylase and P450 aromatase genes in the chicken gonad before and after sexual differentiation 査読

    Yoshida, K, Shimada, K, Saito, N

    General and Comparative Endocrinology102 ( 2 ) 233 - 240   1996年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1006/gcen.1996.0064

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  • Effects of aromatase inhibitor on sex differentiation and levels of P450<sub>17α</sub> and P450 arom messenger ribonucleic acid of gonads in chicken embryos 査読

    Abinawanto, Shimada, K, Yoshida, K, Saito, N

    General and Comparative Endocrinology102 ( 2 ) 241 - 246   1996年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1006/gcen.1996.0065

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  • Triiodothyronine (T3) Enhances the Stimulatory Effect of 1, 25-Dihydroxyvitamin D3 on Calbindin-D28K mRNA Expression in the Kidney and Intestine but Not in Cerebellum of the Chick 査読

    A. Sechman, K. Shimada, N. Saito, T. Ieda, T. Ono

    Asian-Australasian Journal of Animal Sciences9 ( 1 ) 37 - 44   1996年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Asian-Australasian Association of Animal Production Societies  

    The present study was conducted to investigate the role of thyroid hormones in the regulation of gene expression of calbindin-D28k (CaBP-D28K) in the chicken. By employing slot blot and RIA analyses, levels of CaBP-D28K mRNA and CaBP-D28K protein in the intestine, kidney, cerebellum and liver were measured 6 and 12 h after i.m. injection of 1, 25-dihydroxyvitamin D3 [1, 25 (OH)2D3
    250 ng/chick] and 3, 5, 3′-triiodothyronine (T3
    500 ng/chick) in one-day-old chicks. The abundant messages of CaBP-D28K mRNA were detected in the intestine, kidney and cerebellum while there was little message in the liver. After 1, 25 (OH)2D3 treatment (6 + 12 hours), levels of CaBP-D28K mRNA increased in the intestine, but there was no change in the mRNA levels in the kidney and cerebellum. Although T3 alone had no effect on CaBP-D28K mRNA levels, simultaneous administration of T3 enhanced the. 1, 25 (OH)2D3 effect on levels of CaBP-D28K mRNA in the intestine both 6 and 12 h post-treatment, and in the kidney 12 h post-treatment. At a protein level, co-treatment with 1, 25 (OH)2D3 and T3 elicited a significant increase in CaBP-D28K expression in the intestine 12 h post-treatment, as compared to treatment with only 1, 25 (OH)2D3, whereas no differences were observed in the CaBP-D28K protein levels in the kidney and cerebellum. These results suggest that thyroid hormones may play a synergistic role with 1, 25 (OH)2D3 for CaBP-D28K gene expression in the intestine and kidney in chicks.

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  • EFFECTS OF PRESENCE OF AN EGG AND CALCIUM DEPOSITION IN THE SHELL GLAND ON LEVELS OF MESSENGER-RIBONUCLEIC-ACID OF CABP-D-28K AND OF VITAMIN-D-3 RECEPTOR IN THE SHELL GLAND OF THE LAYING HEN 査読

    T IEDA, N SAITO, T ONO, K SHIMADA

    GENERAL AND COMPARATIVE ENDOCRINOLOGY99 ( 2 ) 145 - 151   1995年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Levels of calbindin-D-28K (CaBP-D-28K) mRNA and vitamin D-3, 1(alpha),25(OH)(2)D-3, receptor (VDR) mRNA in the intestine and shell gland were measured in the laying hen during the ovulatory cycle by Northern and slot blot analyses. In addition, effects of presence of an egg and calcium deposition in the shell gland on the levels of both mRNAs were studied either by delay in oviposition (retaining the egg in the shell gland beyond the expected time of oviposition) or premature oviposition (emptying the shell gland and arrest of calcium deposition). During the ovulatory cycle mRNA levels of CaBP-D-28K and of VDR remained relatively constant in the intestine. In contrast, both mRNA levels of the shell gland were low when there was an egg in the infundibulum, magnum, and isthmus, but significantly increased when there was an egg in the shell gland during shell formation. The im injection of indomethacin 3 hr before expected ovulation delayed oviposition but increased neither shell thickness nor mRNA levels of CaBP-D-28K and of VDR. On the other hand, premature oviposition reduced mRNA levels of both CaBP-D-28K and VDR in the shell gland. These results suggest that the presence of an egg and calcium deposition in the shell gland may be a stimulatory factor for synthesis and accumulation of CaBP-D-28K mRNA and VDR mRNA in association with calcification. (C) 1995 Academic Press, lnc.

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  • EFFECTS OF PRESENCE OF AN EGG AND CALCIUM DEPOSITION IN THE SHELL GLAND ON LEVELS OF MESSENGER-RIBONUCLEIC-ACID OF CABP-D-28K AND OF VITAMIN-D-3 RECEPTOR IN THE SHELL GLAND OF THE LAYING HEN 査読

    T IEDA, N SAITO, T ONO, K SHIMADA

    GENERAL AND COMPARATIVE ENDOCRINOLOGY99 ( 2 ) 145 - 151   1995年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Levels of calbindin-D-28K (CaBP-D-28K) mRNA and vitamin D-3, 1(alpha),25(OH)(2)D-3, receptor (VDR) mRNA in the intestine and shell gland were measured in the laying hen during the ovulatory cycle by Northern and slot blot analyses. In addition, effects of presence of an egg and calcium deposition in the shell gland on the levels of both mRNAs were studied either by delay in oviposition (retaining the egg in the shell gland beyond the expected time of oviposition) or premature oviposition (emptying the shell gland and arrest of calcium deposition). During the ovulatory cycle mRNA levels of CaBP-D-28K and of VDR remained relatively constant in the intestine. In contrast, both mRNA levels of the shell gland were low when there was an egg in the infundibulum, magnum, and isthmus, but significantly increased when there was an egg in the shell gland during shell formation. The im injection of indomethacin 3 hr before expected ovulation delayed oviposition but increased neither shell thickness nor mRNA levels of CaBP-D-28K and of VDR. On the other hand, premature oviposition reduced mRNA levels of both CaBP-D-28K and VDR in the shell gland. These results suggest that the presence of an egg and calcium deposition in the shell gland may be a stimulatory factor for synthesis and accumulation of CaBP-D-28K mRNA and VDR mRNA in association with calcification. (C) 1995 Academic Press, lnc.

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  • REGIONALIZED GENE-EXPRESSION OF PROLACTIN AND GROWTH-HORMONE IN THE CHICKEN ANTERIOR-PITUITARY GLAND 査読

    N KANSAKU, K SHIMADA, N SAITO

    GENERAL AND COMPARATIVE ENDOCRINOLOGY99 ( 1 ) 60 - 68   1995年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Messenger ribonucleic acid (mRNA) levels of PRL and GH in perifused cephalic and caudal lobes of the anterior pituitary gland of the chicken were determined by Northern blot analysis and are related to hypothalamic peptide perifusion. Perifusion of vasoactive intestinal peptide (VIP, 10 and 100 nM) significantly increased both PRL secretion and levels of PRL mRNA in the cephalic lobe, whereas no effects of VIP were observed in the caudal lobe. In contrast, growth hormone-releasing factor (GRF) (1-100 nM) did not induce an increase in either GH secretion or levels of GH mRNA in the cephalic lobe but did increase GH secretion and levels of GPI mRNA in the caudal lobe. In addition, forskolin perifusion increased secretion and levels of PRL and GH mRNA in both cephalic and caudal lobes, respectively. These results indicate that gene expression of PRL and GH in response to VIP and GRF, respectively, is localized to specific lobes of the anterior pituitary gland, but that they may share, in part, a common second messenger pathway, namely, cAMP. (C) 1995 Academic Press, Inc.

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  • REGIONALIZED GENE-EXPRESSION OF PROLACTIN AND GROWTH-HORMONE IN THE CHICKEN ANTERIOR-PITUITARY GLAND 査読

    N KANSAKU, K SHIMADA, N SAITO

    GENERAL AND COMPARATIVE ENDOCRINOLOGY99 ( 1 ) 60 - 68   1995年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Messenger ribonucleic acid (mRNA) levels of PRL and GH in perifused cephalic and caudal lobes of the anterior pituitary gland of the chicken were determined by Northern blot analysis and are related to hypothalamic peptide perifusion. Perifusion of vasoactive intestinal peptide (VIP, 10 and 100 nM) significantly increased both PRL secretion and levels of PRL mRNA in the cephalic lobe, whereas no effects of VIP were observed in the caudal lobe. In contrast, growth hormone-releasing factor (GRF) (1-100 nM) did not induce an increase in either GH secretion or levels of GH mRNA in the cephalic lobe but did increase GH secretion and levels of GPI mRNA in the caudal lobe. In addition, forskolin perifusion increased secretion and levels of PRL and GH mRNA in both cephalic and caudal lobes, respectively. These results indicate that gene expression of PRL and GH in response to VIP and GRF, respectively, is localized to specific lobes of the anterior pituitary gland, but that they may share, in part, a common second messenger pathway, namely, cAMP. (C) 1995 Academic Press, Inc.

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  • Expression of P450 17α hydroxylase and P450 aromatase genes in isolated granulosa, theca interna and theca externa layers of the chicken ovarian follicles during follicular growth 査読

    Kato, M, Shimada, K, Saito, N, Noda, K, Ohta, M

    Biology of Reproduction52 ( 2 ) 405 - 410   1995年2月

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  • PROLACTIN, GROWTH-HORMONE, AND LUTEINIZING HORMONE-BETA SUBUNIT GENE-EXPRESSION IN THE CEPHALIC AND CAUDAL LOBES OF THE ANTERIOR-PITUITARY GLAND DURING EMBRYOGENESIS AND DIFFERENT REPRODUCTIVE STAGES IN THE CHICKEN 査読

    N KANSAKU, K SHIMADA, O TERADA, N SAITO

    GENERAL AND COMPARATIVE ENDOCRINOLOGY96 ( 2 ) 197 - 205   1994年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Messenger RNA (mRNA) levels of prolactin (PRL), growth hormone (GH), and luteinizing hormone (LH)-beta subunit in the cephalic and caudal lobes of the anterior pituitary gland of the chicken during embryonic development and different reproductive stages were determined by Northern blot analysis and are related to hormone levels in plasma and each lobe. Steady-state levels of 1.4-kb transcript of PRL of the cephalic and caudal lobes increased on Days 18 and 20 of incubation, respectively. The levels were greater in the cephalic lobe than in the caudal lobe on Days 18 and 20 of incubation. Levels of PRL in each lobe show similar changes to those of mRNA levels. GH mRNA levels of both lobes started to increase on Day 16 of incubation but the levels were higher in the caudal lobe than in the cephalic lobe on Days 16-20 of incubation. Levels of GH in the cephalic lobe increased discontinuously, while those in the caudal lobe increased continuously on days of incubation. LH-beta mRNA was not detected in embryos. Levels of PRL mRNA increased approximately 18-, 20-, and 3-fold in hens at 1 and 3 weeks of egg incubation and brooding stages in the hen, relative to prelaying, respectively. These levels were higher in the cephalic lobe than in the caudal lobe at all stages except for prelaying stages. Levels of PRL in each lobe displayed essentially similar changes to the mRNA levels. GH mRNA levels did not significantly vary during the cycle but were higher in the caudal lobe than in the cephalic lobe. There was no difference in LH-beta mRNA levels between the two lobes but the levels were higher at prelaying and laying stages than at incubation and brooding stages. The same changes in LH concentrations in plasma as those of LH-beta mRNA were found at the stages. This study shows regional differences between PRL and GH mRNA levels in the anterior pituitary gland in relation to embryogenesis and different reproductive stages. (C) 1994 Academic Press, Inc.

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  • TISSUE-SPECIFIC EXPRESSION OF CALBINDIN-D-28K GENE DURING ONTOGENY OF THE CHICKEN 査読

    A SECHMAN, K SHIMADA, N SAITO, T IEDA, T ONO

    JOURNAL OF EXPERIMENTAL ZOOLOGY269 ( 5 ) 450 - 457   1994年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    The vitamin D-3-dependent calcium binding protein, calbindin-D28K (CaBP-D28K), plays an important role in transepithelial calcium translocation. To evaluate its role in chick embryonic calcium metabolism, steady-state levels of CaBP-D28K mRNA in various tissues of the chick embryo were determined by Northern blot and slot blot analyses, and CaBP-D28K concentrations in the examined tissues and plasma were estimated by RIA. High levels of CaBP-D28K mRNA were found in the embryonic kidney (mesonephros) on embryonic day (E) 10 and E12 and thereafter gradually decreased until hatching. CaBP-D28K mRNA levels were low in the yolk sac until E16 but increased on E18 and reached a maximum on E20. A steady increase in CaBP-D28K mRNA levels was observed in the cerebellum during the development from E10 to post-hatching. CaBP-D28K mRNA levels in the intestine were very low during the incubation period but significantly increased on days 1 and 7 after hatching. By Northern blot analysis, CaBP-D28K mRNA was barely detectable in liver, heart, and chorioallantoic membrane of the embryonic chick. Changes in immunoreactive CaBP-D28K of each tissue paralleled observed changes in mRNA levels. In plasma, measurable levels of CaBP-D28K were found as early as E8 and were stable until E18, when 6.5-fold increase was observed compared to E16. The highest level of CaBP-D28K in plasma was found on E20 and decreased after hatching. These temporal profiles of CaBP-D28K suggest that it may play an important role in the regulation of chick embryonic calcium homeostasis. (C) 1994 Wiley-Liss, Inc.

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  • PITUITARY AND PLASMA ARGININE VASOTOCIN LEVELS IN THE LAMPREY, LAMPETRA-JAPONICA 査読

    M UCHIYAMA, N SAITO, K SHIMADA, T MURAKAMI

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY107 ( 1 ) 23 - 26   1994年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Pituitary and plasma arginine vasotocin (AVT) concentrations were examined in correlation with plasma mineral concentrations in the lamprey acclimated to freshwater (FW) and diluted seawater (SW). There were significant differences in plasma osmolality and plasma concentrations of sodium, chloride, calcium and magnesium between lampreys acclimated to FW and those acclimated to diluted SW. Although the plasma AVT concentration in the FW group was not significantly different from that in the diluted SW group, the pituitary AVT content in the FW group was significantly higher than that in the SW group. These results seem to show that synthesis of AVT is increased in FW lampreys.

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  • High-level expression of biologically active chicken prolactin in E. coli. 査読

    Ohkubo, T, Tanaka, M, Nakashima, K, Shimada, K. Saito, N, Sato, K

    Comparative Biochemistry and Physiology105A   123 - 128   1993年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • ALTERATIONS IN UTERINE CONTRACTILITY DURING THE OVIPOSITION CYCLE IN DOMESTIC HENS 査読

    N SAITO, TI KOIKE

    BRITISH POULTRY SCIENCE33 ( 3 ) 671 - 676   1992年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CARFAX PUBL CO  

    1. The potencies of several neurohypophysial hormones were examined at different times during the oviposition cycle in an in vitro fowl oxytocic assay.
    2. Uterine tissues were removed 2 h before (-2h-OP), immediately after (0h-OP) and 5 h after (+5h-OP) spontaneous oviposition. In addition, uterine tissue was removed immediately after oviposition was induced, by administering prostaglandin E2 2 h before an expected oviposition (Induced-OP).
    3. The rank order of oxytocic potencies for the peptides was arginine vasotocin=vasopressin&gt;oxytocin&gt;mesotocin. The sensitivity of the uterus to the hormones was 0h-OP = Induced-OP &gt; -2h-OP = +5-OP.
    4. These results suggest that uterine sensitivity to neurohypophysial hormones changes during the oviposition cycle in domestic fowls.

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  • Effects of removal of chicks from hens or concentrations of prolactin, luteinizing hormone and oestradiol in plasma of broody Gifu jidori hens. 査読

    Kuwayama, T, Shimada, K, Saito, N, Ohkubo, T, Sato, K, Wada, M, Ichinoe, K

    Journal of Reproduction & Fertility95   617 - 622   1992年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Seasonal changes in the repreoductive function of Java sparrous (Padda oryzinora) 査読

    Saito, N, Shimada, K, Nomura, N, Oguri, K, Sato, K, Wada, M

    Comparative Biochem. Physiology101A   459 - 463   1992年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Changes in body weight, egg production, Haclkle growth, and plasma sex steroid hormones and prolactin during the annual reproduction cycle in domestic geese. 査読

    Izumi, T, Shimada, K, Saito, N, Ishida, H, Sato, K, Uchide, K, Tomita, Y, Sakakida, S, Nishida, E

    Japanese Poultry Science29 ( 6 ) 378 - 388   1992年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.29.378

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  • ARGININE VASOTOCIN AND MESOTOCIN LEVELS IN THECA AND GRANULOSA LAYERS OF THE OVARY DURING THE OVIPOSITION CYCLE IN HENS (GALLUS-DOMESTICUS) 査読

    N SAITO, S KINZLER, TI KOIKE

    GENERAL AND COMPARATIVE ENDOCRINOLOGY79 ( 1 ) 54 - 63   1990年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

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  • Ovarian prostaglandin levels at the midsequence oviposition, the terminal oviponsition and the first ovulation of a sequence in the hen 査読

    Saito, N, Shimada, K

    Japanese Poultry Science25   296 - 304   1988年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.25.296

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  • Prostaglandin levels in plasma and follicular and uterine tissues of the quail in relation to midsequence oviposition 査読

    Saito, N, Shimada, K

    Japanese Poultry Science25   261 - 267   1988年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.25.261

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  • INTERRELATIONSHIP BETWEEN ARGININE VASOTOCIN, PROSTAGLANDIN, AND UTERINE CONTRACTILITY IN THE CONTROL OF OVIPOSITION IN THE HEN (GALLUS-DOMESTICUS) 査読

    N SAITO, K SHIMADA, TI KOIKE

    GENERAL AND COMPARATIVE ENDOCRINOLOGY67 ( 3 ) 342 - 347   1987年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

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  • Prostaglandin levels in peripheral and follicular plasma, the isolated theca and granulosa layers of the shell gland (uterus) during a midsequance-oviposition cycle of the hen(Gallus domesticus) 査読

    Saito, N, Sato, K, Shimada, K

    Biology of Reproduction36   89 - 96   1987年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Changes plasma concentration of arginine vasotocin after injection of prostaglandine for and cetylcholine at various time during the oviposivtion cycle of the donestic hen 査読

    Shimada, K, Saito, N, Itogawa, K, Koike, T. I

    J. Reproduction Fertility80   143 - 150   1987年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • ONTOGENIC STEROIDOGENESIS BY TESTES, OVARY, AND ADRENALS OF EMBRYONIC AND POSTEMBRYONIC CHICKENS (GALLUS-DOMESTICUS) 査読

    Y TANABE, N SAITO, T NAKAMURA

    GENERAL AND COMPARATIVE ENDOCRINOLOGY63 ( 3 ) 456 - 463   1986年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

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  • Prolonged inhibitory effect of progestorone on ovulation in the domestic hen 査読

    Saito, N, Tsuzuki, N, Shimada, K

    Japanese Poultry Science23   211 - 221   1986年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2141/jpsa.23.211

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  • DISTRIBUTION OF LHRH-LIKE IMMUNOREACTIVITY IN THE BRAIN OF THE JAPANESE EEL (ANGUILLA-JAPONICA) WITH SPECIAL REFERENCE TO THE NERVUS TERMINALIS 査読

    M NOZAKI, FUJITA, I, N SAITO, T TSUKAHARA, H KOBAYASHI, K UEDA, K OSHIMA

    ZOOLOGICAL SCIENCE2 ( 4 ) 537 - 547   1985年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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書籍等出版物

  • ホルモンハンドブック新訂ebook版

    南江堂  2007年 

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  • Dimorphic mRNA expression of P450arom and AMH effects of estradiol in the chicken embryo.

    Recent Progress in Molecular and Comparative Endocrinology.  1999年 

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  • Effects of opioid peptides on neurohypophysial hormone release in chicks.

    Recent Progress in Molecular and Comparative Endocrinology.  1999年 

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  • Dimorphic mRNA expression of P450arom and AMH effects of estradiol in the chicken embryo.

    Recent Progress in Molecular and Comparative Endocrinology.  1999年 

     詳細を見る

  • Effects of opioid peptides on neurohypophysial hormone release in chicks.

    Recent Progress in Molecular and Comparative Endocrinology.  1999年 

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  • Molecular mechanisms of sex determination and sex differentiation.

    Reproductive Biology Up date -novel tools for assessment of environmental toxicity.  1998年 

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  • Molecular mechanisms of sex determination and sex differentiation.

    Reproductive Biology Up date -novel tools for assessment of environmental toxicity.  1998年 

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  • Control of ovarian steroidogenesis: gene expression of steroidogenic enzymes.

    Perspectives in Avian Endocrinology  1997年 

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  • Control of ovarian steroidogenesis: gene expression of steroidogenic enzymes.

    Perspectives in Avian Endocrinology  1997年 

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  • The molecular biology of prolactin.

    Avian Endocrinology  1993年 

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  • Ovarian prostaglondin and arginine vasotocin in relation to oviposition

    Avian Endocrinology  1993年 

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  • Ovarian prostaglondin and arginine vasotocin in relation to oviposition

    Avian Endocrinology  1993年 

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  • The molecular biology of prolactin.

    Avian Endocrinology  1993年 

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MISC

  • In vivo and in vitro screening of endocrine disrupting chemicals with estrogenic activity in Japanese quail

    K Shimada, Y Ha, A Tsukada, N Saito, S Maekawa, M Nishizuka, M Imagawa

    AVIAN AND POULTRY BIOLOGY REVIEWS16 ( 1 ) 19 - 27   2005年

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    記述言語:英語   出版者・発行元:SCIENCE & TECHNOLOGY LETTERS  

    This review paper describes the (1) establishment of an in vitro method for screening endocrine disrupting chemicals with estrogenic activity and (2) study on effects of nonylphenol (NP) and octylphenol (OP) on m RNA expression of vitellogenin (VTG) II and very low density lipoprotein (apoVLDL) II in the liver of quail embryos. For in vitro screening, the cDNA of the ligand binding domain of quail estrogen receptor (ER) &alpha; or quail &beta; was ligated into the gluthathione S-transferase expression vector and expressed in E. coli. The fusion protein was used for competitive enzyme immunoassay. Of the 20 compounds examined, nine and five showed binding to ER &alpha; and ER &beta;, respectively. NP and OP showed the highest binding to ER &alpha;, and bisphenol-A to ER &beta;, with binding affinities relative to that of diethylstilbesterol (DES; 10(-9) M) of 6% and 6.7%, respectively. For the in vivo study, quail fertile eggs were treated with a single injection of either NP or OP (10 and 100 nmole/egg, in 20 &mu; l) on day 13 of incubation. VTGII and apoVLDLII mRNA levels on day 15 of incubation were determined by RT-PCR. A weak but distinct expression of VTG II and ApcVLDLII mRNAwas observed after NP and OP, indicating that NP and OP could be endocrine disruptors; in birds.

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  • Avian Endocrinology

    Narosa Publishing House   155 - 166   2001年

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  • Reproductive Biotechnology

    Hokuto Shobo   391 - 395   2001年

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  • Avian Endocrinology

    Narosa Publishing House   155 - 166   2001年

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  • Recent Progress in Molecular and Comparative Endocrinology

    Hormone Research Center   573 - 578   1999年

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  • Recent Progress in Molecular and Comparative Endocrinology

    Hormone Research Center   218 - 221   1999年

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  • Recent Progress in Molecular and Comparative Endocrinology

    Hormone Research Center   573 - 578   1999年

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  • Recent Progress in Molecular and Comparative Endocrinology

    Hormone Research Center   218 - 221   1999年

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  • Reproductive Biology Update -novel tools for assessment of enviromental toxicity

    Shoukadoh Booksellers Company   3 - 11   1998年

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  • Reproductive Biology Update -novel tools for assessment of enviromental toxicity

    Shoukadoh Booksellers Company   3 - 11   1998年

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  • Perspectives in Avian Endocrinology

    Journal of Endocrinology Ltd.   193 - 200   1997年

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  • "新しい卵巣ホルモン"の産生と作用

    斉藤 昇

    日本家禽学会誌34 ( 1 ) 1 - 8   1997年

  • """新しい卵巣ホルモン""の産生と作用"

    日本家禽学会誌34   1-8.   1997年

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  • Perspectives in Avian Endocrinology

    Journal of Endocrinology Ltd.   193 - 200   1997年

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  • 産卵制御の分子機構

    学術月報48   578 - 586   1995年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(その他)  

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  • Control of ovposition

    Critical Revieus in Poulty Biology   2   1989年

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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Works(作品等)

  • ニワトリの水分摂取・排泄の生理機構解明と家禽管理への展開

    2005年
    -
    2007年

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  • 鳥類の浸透圧調節機構に関する研究

    2002年
    -
    2004年

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  • 鳥類Z性染色体上Dmrt-1遺伝子の雄性決定因子としての機能解析

    2001年
    -
    2002年

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  • 鳥類の脳内浸透圧受容体と下垂体神経葉ホルモンの分泌調節機構

    2000年
    -
    2001年

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  • 鳥類における浸透圧受容体について

    2000年

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  • 鳥類の性決定分子機構

    1998年
    -
    2000年

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  • 自然界に存在しないW精子の機能の検定と応用研究

    1998年
    -
    2000年

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  • 性決定・性分化の分子機構に関する研究

    1998年
    -
    1999年

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  • 下垂体神経葉ホルモンの生理作用の解明とその比較動物学的検討

    1997年
    -
    1998年

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  • 孵卵行動発現の分子生物学的解明と遺伝子操作による発現の阻止

    1995年
    -
    1997年

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受賞

  • 日本家禽学会奨励賞

    1995年  

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    受賞国:日本国

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共同研究・競争的資金等の研究

  • 鳥類の脳の雌性化機構とアロマターゼ遺伝子発現調節機構の解明

    2014年 - 2016年

    科研費補助金 

    齋藤 昇

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    資金種別:競争的資金

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  • 抗利尿ホルモン遺伝子発現調節機構の解明

    2011年 - 2013年

    齋藤 昇

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    資金種別:競争的資金

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  • ニワトリ野生原種の保全と多種系統の基盤遺伝特性の解析による研究利用性の拡大

    2008年 - 2010年

    並河 鷹夫

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    資金種別:競争的資金

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  • ブロイラーとレイヤーにおける脳機能の違いの解明とそれを応用した飼養技術の導入

    2005年 - 2007年

    菅原 邦生

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    資金種別:競争的資金

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  • ニワトリの水分摂取・排泄の生理機構解明と家禽管理への展開

    2005年 - 2007年

    齋藤 昇

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    資金種別:競争的資金

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  • Mechanisms of sexual differentiation and gonadal development in birds

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    資金種別:競争的資金

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