Updated on 2024/12/23

写真a

 
Motoki Ko
 
Organization
Faculty of Environmental, Life, Natural Science and Technology Assistant Professor
Position
Assistant Professor
Homepage
https://www.elst.okayama-u.ac.jp/education/mc/chikyu/areas09_plant/
External link

Degree

  • Doctor of Agriculture ( 2022.7   Kyoto University )

Research History

  • 岡山大学 学術研究院 環境生命自然科学学域   助教

    2023.4

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  • 京都大学農学研究科 附属農場   助教

    2019.8 - 2023.3

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  • 京都大学大学院 農学研究科 博士後期課程

    2018.4 - 2019.7

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  • 京都大学大学院 農学研究科 博士前期課程

    2016.4 - 2018.3

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  • 京都大学 農学部 資源生物科学科

    2011.4 - 2016.3

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Papers

  • Development of an AI-based Image Analysis System to Calculate the Visit Duration of a Green Blow Fly on a Strawberry Flower Reviewed

    Hiroki Taniguchi, Yuki Tsukuda, Ko Motoki, Tanjuro Goto, Yuichi Yoshida, Ken‑ichiro Yasuba

    The Horticulture Journal   2025

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    Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hortj.qh-159

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  • A low-cost dpMIG-seq method for elucidating complex inheritance in polysomic crops: A case study in tetraploid blueberry Reviewed

    Kyoka Nagasaka, Kazusa Nishimura, Ko Motoki, Keigo Yamagata, Soichiro Nishiyama, Hisayo Yamane, Ryutaro Tao, Ryohei Nakano, Tetsuya Nakazaki

    Horticulture Research   2024.9

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    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    Next-generation sequencing (NGS) library construction often requires high-quality DNA extraction, precise adjustment of DNA concentration, and restriction enzyme digestion to reduce genome complexity, which results in increased time and cost in sample preparation and processing. To address these challenges, a PCR-based method for rapid NGS library preparation, named dpMIG-seq, has been developed and proven effective for high-throughput genotyping. However, the application of dpMIG-seq has been limited to diploid and polyploid species with disomic inheritance. In this study, we obtained genome-wide single nucleotide polymorphism (SNP) markers for tetraploid blueberry to evaluate genotyping and downstream analysis outcomes. Comparison of genotyping qualities inferred across samples with different DNA concentrations and multiple bioinformatics approaches revealed high accuracy and reproducibility of dpMIG-seq-based genotyping, with Pearson’s correlation coefficients between replicates in the range of 0.91 to 0.98. Furthermore, we demonstrated that dpMIG-seq enables accurate genotyping of samples with low DNA concentrations. Subsequently, we applied dpMIG-seq to a tetraploid F1 population to examine the inheritance probability of parental alleles. Pairing configuration analysis supported the random meiotic pairing of homologous chromosomes on a genome-wide level. On the other hand, preferential pairing was observed on chr-11, suggesting that there may be an exception to the random pairing. Genotypic data suggested quadrivalent formation within the population, although the frequency of quadrivalent formation varied by chromosome and cultivar. Collectively, the results confirmed applicability of dpMIG-seq for allele dosage genotyping and are expected to catalyze the adoption of this cost-effective and rapid genotyping technology in polyploid studies.

    DOI： 10.1093/hr/uhae248

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  • Development of the “GRAFT method”, a rapid floral induction technique of cruciferous crops by grafting onto radish rootstock Invited

    Regulation of Plant Growth & Development   59 ( 1 )   46 - 54   2024.7

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    Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

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  • Utilization of lysed and dried bacterial biomass from the marine purple photosynthetic bacterium Rhodovulum sulfidophilum as a sustainable nitrogen fertilizer for plant production Reviewed

    Shamitha Rao Morey-Yagi, Yu Kinoshita, Ko Motoki, Yu Iwahashi, Dao Duy Hanh, Shota Kato, Ryohei Nakano, Kumiko Ochiai, Masaru Kobayashi, Tetsuya Nakazaki, Keiji Numata

    npj Sustainable Agriculture   2 ( 1 )   2024.6

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Plant-based agricultural systems rely heavily on inorganic nitrogen (N) fertilizers to increase yields and ensure food security for the rapidly growing global population. However, the production and overuse of synthetic fertilizers lead to significant amounts of greenhouse gas emissions, causing a critical need for the development of alternative and sustainable organic N fertilizers. Here, we demonstrate the effective use of lysed and dried bacterial biomass from the marine purple photosynthetic bacterium Rhodovulum sulfidophilum as an alternative source of N fertilizer for the cultivation of Japanese mustard spinach (komatsuna, Brassica rapa var. perviridis). To assess the suitability of this processed bacterial biomass, containing approximately 11% N, as a N fertilizer, we examined the effects of its application on plant germination and growth (measured by leaf chlorophyll, maximum leaf length, and dry weight) under two different temperature regimes, comparing it to that of a conventional N-containing mineral fertilizer. Application at rates up to four times that of the mineral fertilizer had no negative effects on seed germination and plant growth. The bacterial biomass had to be applied at approximately twice the rate of mineral fertilizer to obtain similar plant growth parameters, roughly corresponding to the predicted 62% rate of mineralization of the bacterial N. Our findings confirm the ability of plants to take up N from the lysed and dried biomass of marine purple photosynthetic bacteria, demonstrating the potential for using R. sulfidophilum as a source of N fertilizer.

    DOI： 10.1038/s44264-024-00018-0

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    Other Link： https://www.nature.com/articles/s44264-024-00018-0

  • Degenerate oligonucleotide primer <scp>MIG</scp>‐seq: an effective <scp>PCR</scp>‐based method for high‐throughput genotyping Reviewed

    Kazusa Nishimura, Hiroyuki Kokaji, Ko Motoki, Akira Yamazaki, Kyoka Nagasaka, Takashi Mori, Rihito Takisawa, Yasuo Yasui, Takashi Kawai, Koichiro Ushijima, Masanori Yamasaki, Hiroki Saito, Ryohei Nakano, Tetsuya Nakazaki

    The Plant Journal   118 ( 6 )   2296 - 2317   2024.3

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    SUMMARY

    Next‐generation sequencing (NGS) library construction often involves using restriction enzymes to decrease genome complexity, enabling versatile polymorphism detection in plants. However, plant leaves frequently contain impurities, such as polyphenols, necessitating DNA purification before enzymatic reactions. To overcome this problem, we developed a PCR‐based method for expeditious NGS library preparation, offering flexibility in number of detected polymorphisms. By substituting a segment of the simple sequence repeat sequence in the MIG‐seq primer set (MIG‐seq being a PCR method enabling library construction with low‐quality DNA) with degenerate oligonucleotides, we introduced variability in detectable polymorphisms across various crops. This innovation, named degenerate oligonucleotide primer MIG‐seq (dpMIG‐seq), enabled a streamlined protocol for constructing dpMIG‐seq libraries from unpurified DNA, which was implemented stably in several crop species, including fruit trees. Furthermore, dpMIG‐seq facilitated efficient lineage selection in wheat and enabled linkage map construction and quantitative trait loci analysis in tomato, rice, and soybean without necessitating DNA concentration adjustments. These findings underscore the potential of the dpMIG‐seq protocol for advancing genetic analyses across diverse plant species.

    DOI： 10.1111/tpj.16708

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  • Pollen Dispersion is a Key Factor for Autonomous Fruit Set Under High Temperatures in the Capsicum annuum ‘Takanotsume’ Reviewed

    Akira Yamazaki, Ao Takezawa, Kazusa Nishimura, Ko Motoki, Kyoka Nagasaka, Ryohei Nakano, Tetsuya Nakazaki, Munetaka Hosokawa

    The Horticulture Journal   2024

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    Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hortj.qh-094

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  • Elucidation of Low-temperature Regulated Flavone Synthesis in &lt;i&gt;Dahlia Variabilis&lt;/i&gt; and its Effects on Flower Color Reviewed

    Edna K. Muthamia, Koji Naito, Hiromasa Okada, Yukino Karasawa, Tokuyu Kikumura, Takuya Nara, Yasunori Hamauzu, Ko Motoki, Ken-ichiro Yasuba, Yuichi Yoshida, Yoshikuni Kitamura, Tanjuro Goto

    The Horticulture Journal   93 ( 4 )   335 - 343   2024

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    Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hortj.qh-144

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  • RoseTracker: A system for automated rose growth monitoring Reviewed

    Risa Shinoda, Ko Motoki, Kensho Hara, Hirokatsu Kataoka, Ryohei Nakano, Tetsuya Nakazaki, Ryozo Noguchi

    Smart Agricultural Technology   5   100271 - 100271   2023.10

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.atech.2023.100271

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  • A simple method for measuring pollen germination rate using machine learning Reviewed

    Akira Yamazaki, Ao Takezawa, Kyoka Nagasaka, Ko Motoki, Kazusa Nishimura, Ryohei Nakano, Tetsuya Nakazaki

    Plant Reproduction   36 ( 4 )   355 - 364   2023.6

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00497-023-00472-9

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    Other Link： https://link.springer.com/article/10.1007/s00497-023-00472-9/fulltext.html

  • Upregulation of tandem duplicated BoFLC1 genes is associated with the non-flowering trait in Brassica oleracea var. capitata Reviewed

    Yu Kinoshita, Ko Motoki, Munetaka Hosokawa

    Theoretical and Applied Genetics   136 ( 3 )   2023.3

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00122-023-04311-3

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    Other Link： https://link.springer.com/article/10.1007/s00122-023-04311-3/fulltext.html

  • Indicator Candidate Traits for Autonomous Fruit Set Ability Under High Temperatures in Capsicum Reviewed

    Akira Yamazaki, Ao Takezawa, Ryohei Nakano, Kazusa Nishimura, Ko Motoki, Munetaka Hosokawa, Tetsuya Nakazaki

    Journal of Horticultural Research   30 ( 2 )   105 - 116   2023.1

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    Publishing type：Research paper (scientific journal)   Publisher：Walter de Gruyter GmbH  

    Abstract

    “Autonomous fruit set” refers to self-pollination and fruit set without pollen vectors such as vibration or insects. Autonomous fruit set under high-temperature stress is an important breeding goal as climate change can reduce fruit yields in Capsicum. We screened Capsicum cultivars for autonomous fruit set ability in a greenhouse environment and investigated pollen germination, viability, pollen grains number, chlorophyll fluorescence (Fv/Fm), style length, anther cone length, and anthesis stage under high temperatures in order to identify indicator traits for screening more genotypes with autonomous fruit set ability. The fruit set of the ‘Takanotsume’ (57.7 ± 20.6%) and ‘Goshiki Kyokko’ (52.2 ± 14.2%) cultivars (both C. annuum) were higher than those of other cultivars. Correlation analysis showed that pollen germination had the highest correlation with fruit set in C. annuum cultivars (r = 0.63). These results indicate that ‘Takanotsume’ and ‘Goshiki Kyokko’ are useful cultivars for novel breeding programs focusing on autonomous fruit sets under high temperatures, and pollen germination in C. annuum was a convincing candidate for an indicator trait of autonomous fruit set ability under high temperatures.

    DOI： 10.2478/johr-2022-0017

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  • Investigation of the field performance of progenies obtained by a non-vernalization-grafting floral induction method in terms of application to cabbage breeding and seed production Reviewed

    Ko Motoki, Yu Kinoshita, Ryohei Nakano, Munetaka Hosokawa, Tetsuya Nakazaki

    The Horticulture Journal   92 ( 1 )   66 - 76   2023

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hortj.qh-018

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  • Quantitative Analysis of Florigen for the Variability of Floral Induction in Cabbage/Radish Inter-generic Grafting Reviewed

    Ko Motoki, Yu Kinoshita, Ryohei Nakano, Munetaka Hosokawa, Tetsuya Nakazaki

    Plant and Cell Physiology   63 ( 9 )   1230 - 1241   2022.9

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    Grafting-induced flowering is a key phenomenon to understand systemic floral induction caused by florigen. It can also be used as a breeding technique enabling rapid seed production of crops with long generation times. However, the degree of floral induction in grafted plants is often variable. Moreover, it is difficult in some crop species. Here, we explored the factors promoting variability in the grafting-induced flowering of cabbage (Brassica oleracea L. var. capitata), an important vegetable crop with a long generation time, via the quantitative analysis of florigen accumulation. Significant variability in the flowering response of grafted cabbage was observed when rootstocks of different genotypes were used. As reported previously, B. oleracea rootstocks did not induce the flowering of grafted cabbage plants, but radish (Raphanus sativus L.) rootstocks unstably did, depending on the accessions used. Immunoblotting analysis of the FLOWERING LOCUS T (FT) protein, a main component of florigen, revealed that floral induction was quantitatively correlated with the level of accumulated FT protein in the grafted scion. To identify rootstock factors that cause variability in the floral induction of the grafted scion, we investigated FT protein accumulation and flowering response in grafted scions when the transcription levels of FT and the leaf area of rootstocks were altered by vernalization, daylength and leaf trimming treatments. We concluded that increasing the total amount of FT protein produced in the rootstock is important for the stable floral induction of the grafted cabbage, and this can be accomplished by increasing FT transcription and the leaf area of the rootstock.

    DOI： 10.1093/pcp/pcac098

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    Other Link： https://academic.oup.com/pcp/article-pdf/63/9/1230/45852457/pcac098.pdf

  • Analysis of mechanism regulating high total soluble solid content in the parthenocarpic tomato fruit induced by pat-k gene Reviewed

    Chiaki Fukudome, Rihito Takisawa, Ryohei Nakano, Miyako Kusano, Makoto Kobayashi, Ko Motoki, Kazusa Nishimura, Tetsuya Nakazaki

    Scientia Horticulturae   301   111070 - 111070   2022.7

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.scienta.2022.111070

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  • MIG-seq is an effective method for high-throughput genotyping in wheat (Triticum spp.) Reviewed International journal

    Kazusa Nishimura, Ko Motoki, Akira Yamazaki, Rihito Takisawa, Yasuo Yasui, Takashi Kawai, Koichiro Ushijima, Ryohei Nakano, Tetsuya Nakazaki

    DNA Research   29 ( 2 )   2022.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    MIG-seq (Multiplexed inter-simple sequence repeats genotyping by sequencing) has been developed as a low cost genotyping technology, although the number of polymorphisms obtained is assumed to be minimal, resulting in the low application of this technique to analyses of agricultural plants. We applied MIG-seq to 12 plant species that include various crops and investigated the relationship between genome size and the number of bases that can be stably sequenced. The genome size and the number of loci, which can be sequenced by MIG-seq, are positively correlated. This is due to the linkage between genome size and the number of simple sequence repeats (SSRs) through the genome. The applicability of MIG-seq to population structure analysis, linkage mapping, and quantitative trait loci (QTL) analysis in wheat, which has a relatively large genome, was further evaluated. The results of population structure analysis for tetraploid wheat showed the differences among collection sites and subspecies, which agreed with previous findings. Additionally, in wheat biparental mapping populations, over 3,000 SNPs/indels with low deficiency were detected using MIG-seq, and the QTL analysis was able to detect recognized flowering-related genes. These results revealed the effectiveness of MIG-seq for genomic analysis of agricultural plants with large genomes, including wheat.

    DOI： 10.1093/dnares/dsac011

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    Other Link： https://academic.oup.com/dnaresearch/article-pdf/29/2/dsac011/43439343/dsac011.pdf

  • Characterization of a Non-flowering Cabbage Mutant Discovered 42 Years Ago Reviewed

    Yu Kinoshita, Ko Motoki, Munetaka Hosokawa

    The Horticulture Journal   90 ( 4 )   374 - 381   2021

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    Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Horticultural Science  

    DOI： 10.2503/hortj.utd-277

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  • Non-vernalization Flowering and Seed Set of Cabbage Induced by Grafting Onto Radish Rootstocks Reviewed

    Ko Motoki, Yu Kinoshita, Munetaka Hosokawa

    Frontiers in Plant Science   9   2019.1

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    DOI： 10.3389/fpls.2018.01967

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  • Cryopreservation of viroid-infected chrysanthemum shoot tips Reviewed

    Jing-Wei Li, Munetaka Hosokawa, Tomoyuki Nabeshima, Ko Motoki, Haruka Yamada, Qiao-Chun Wang

    Scientia Horticulturae   244   1 - 9   2019.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    Chrysanthemum stunt viroid (CSVd) and chrysanthemum chlorotic mottle viroid (CChMVd) are the two viroids most frequently infecting chrysanthemum. This study attempted to cryopreserve in vitro shoot tips of Chrysanthemum morifolium 'Piato' infected with high or low titers of CSVd, and co-infected with CSVd and CChMVd. By optimizing several key factors including the time and light regimes during cold-hardening of stock shoots, and the size of shoot tips, an encapsulation-vitrification procedure was established for cryopreservation of viroid-infected shoot tips. Viroid-infected stock shoots were cold-hardened in vitro at 4 degrees C in a 16-h photo-period for 6 weeks. Shoot tips (1.5 mm in size) containing 3-4 LPs were excised from the cold-hardened stock shoots and subjected to encapsulation-vitrification cryopreservation. With this protocol, about 65%, 45% and 42% of shoot regrowth levels were obtained in cryopreserved shoot tips derived from in vitro stock shoots infected with low or high titers of CSVd, and co-infected with CSVd and CChMVd, respectively. All regenerants from cryopreservation maintained their viroid-infected status, identical to those of their in vitro stock shoots. Histological observations and in situ hybridization elucidated why cryopreservation could maintain viroids in cryopreserved shoot tips. Cryopreservation of viroid-infected plant materials has potential applications to all types of viroid-related basic and applied studies.

    DOI： 10.1016/j.scienta.2018.09.004

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  • Farmer Research Groups: Institutionalizing participatory agricultural research in Ethiopia (Book review) Reviewed

    Ko Motoki

    Nilo-Ethiopian Studies   2018 ( 23 )   47 - 48   2018

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    Authorship：Lead author,　Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

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MISC

Presentations

  • 接ぎ木を用いたアブラナ科作物の早期開花誘導技術『GRAFT法』の開発 Invited

    元木 航

    第16回 中国地域育種談話会  2024.12.14 

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    Event date： 2024.12.14 - 2024.12.15

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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  • 他者を咲かせる植物をつくる -接ぎ木による開花誘導技術の育種・採種への利用- Invited

    元木 航

    第56回 種生物学シンポジウム  2024.12.7 

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    Event date： 2024.12.6 - 2024.12.8

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • ダイコン台木の接ぎ木花成誘導能力に対する2つのFLC低発現アレルの組み合わせ効果

    小林紗季, 後藤丹十郎, 安場健一郎, 元木 航

    園芸学会令和6年度秋季大会  2024.11.3 

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    Event date： 2024.11.3 - 2024.11.5

    Presentation type：Oral presentation (general)  

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  • 非開花性キャベツ変異体‘不抽苔’におけるBoFLC1遺伝子のサイレンシング阻害に関与するDNAメチル化候補変異の解析

    木下有羽, 元木 航, 青柳優太, 平川英樹, 中野龍平, 細川宗孝

    園芸学会令和6年度秋季大会  2024.11.3 

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    Event date： 2024.11.3 - 2024.11.5

    Presentation type：Oral presentation (general)  

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  • 接ぎ木による開花誘導に対して異なる開花応答を示すキャベツ品種の茎頂における遺伝子発現比較

    元木 航, 森本莉世, 木下有羽, 鹿島 誠, 細川宗孝, 中﨑鉄也, 中野龍平

    園芸学会令和6年度秋季大会  2024.11.3 

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    Event date： 2024.11.3 - 2024.11.5

    Presentation type：Oral presentation (general)  

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  • ダイコンへの接ぎ木によるアブラナ科作物の早期開花誘導技術「GRAFT法」の開発 Invited

    元木 航

    令和6年度野菜花き課題別研究会-アブラナ科野菜の育種・生産を取り巻く現状と展望-  2024.10.22 

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    Event date： 2024.10.22 - 2024.10.23

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Identification of genes contributing to high florigenexpression capacity in radish

    Ko Motoki, Kazusa Nishimura, Makoto Kashima, Tetsuya Nakazaki, Ryohei Nakano, Munetaka Hosokawa

    2024.9.20 

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    Event date： 2024.9.19 - 2024.9.20

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Development of a non-vernalization-grafting floral induction method and suitable rootstocks for rapid breeding of Brassica crops

    Motoki K, Kinoshita Y, Nakano R, Hosokawa M, Nakazaki T

    16th International Rapeseed Congress  2023.9.27 

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    Event date： 2023.9.25 - 2023.9.27

    Language：English   Presentation type：Oral presentation (general)  

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  • Analysis of rootstock factors responsible for the variability of floral induction in cabbage/radish inter-generic grafting

    Ko Motoki, Yu Kinoshita, Ryohei Nakano, Munetaka Hosokawa, Tetsuya Nakazaki

    The 4th Asian Horticultural Congress  2023.8.29 

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    Event date： 2023.8.28 - 2023.8.30

    Language：English   Presentation type：Oral presentation (general)  

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  • Analysis of scion factors responsible for the variability of floral induction in cabbage/radish inter-generic grafting

    Rise Morimoto, Ko Motoki, Kazusa Nishimura, Kyoka Nagasaka, Yu Iwahashi, Hiromo Inoue, Ryohei Nakano, Yu Kinoshita, Munetaka Hosokawa, Tetsuya Nakazaki

    The 4th Asian Horticultural Congress  2023.8.29 

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    Event date： 2023.8.28 - 2023.8.30

    Language：English   Presentation type：Poster presentation  

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  • トウガラシ‘タカノツメ’が有する高温期の自動着果性の遺伝解析

    山崎 彬, 武澤亜緒, 西村和紗, 長坂京香, 岩橋 優, 井上博茂, 元木 航, 中野龍平, 細川宗孝, 中﨑鉄也

    園芸学会令和５年度春季大会  2023.3.19 

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    Event date： 2023.3.19 - 2023.3.20

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  • キャベツ変異体‘不抽苔’の非開花性にはタンデム重複したBoFLC1の発現増加が関与する

    木下有羽, 元木 航, 細川宗孝

    園芸学会令和５年度春季大会  2023.3.19 

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    Event date： 2023.3.19 - 2023.3.20

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  • Development of breeding and seed production technologies using florigen of radish Invited

    2022.11.22 

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    Event date： 2022.11.22

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • FTタンパク質の定量解析によるキャベツの接ぎ木花成誘導条件の解明

    元木 航, 木下有羽, 中野龍平, 細川宗孝, 中﨑鉄也

    園芸学会 令和４年度秋季大会  2022.9.8 

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    Event date： 2022.9.8 - 2022.9.11

    Language：Japanese   Presentation type：Poster presentation  

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  • The mode of inheritance of non-flowering characteristics in cabbage mutant ‘nfc’

    Yu Kinoshita, Ko Motoki, Munetaka Hosokawa

    2022.3.20 

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    Event date： 2022.3.20 - 2022.3.21

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Image recognition of growth stages of flower shoots by acquiring and using overhead video images in a rose greenhouse

    R. Shinoda, K. Motoki, R. Nakano, T. Nakazaki, J. Miyasaka

    2022.3.20 

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    Event date： 2022.3.20 - 2022.3.21

    Language：Japanese  

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  • Development of “Flowering induction rootstock” as a breeding and seed production tool for cruciferous crops Invited

    Ko Motoki

    2021.9.24 

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    Event date： 2021.9.23 - 2021.9.26

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • 育種・採種のツールとして利用可能な”花成誘導台木”の開発

    元木 航, 木下有羽, 中野龍平, 細川宗孝, 中﨑鉄也

    超分野植物科学研究会・第一回研究集会  2021.6.4 

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    Event date： 2021.6.4

    Language：Japanese   Presentation type：Poster presentation  

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  • ダイコンのFTタンパクを検出するペプチド抗体の開発

    元木 航, 木下有羽, 中野龍平, 細川宗孝, 中﨑鉄也

    園芸学会 令和 3 年度春季大会  2021.3.27 

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    Event date： 2021.3.21 - 2021.3.30

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • “花成誘導台木”はキャベツ穂木の種類を問わず開花させることができるのだろうか？

    元木 航, 木下有羽, 細川宗孝

    園芸学会 令和元年度秋季大会  2019.9.15 

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    Event date： 2019.9.15 - 2019.9.17

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • A candidate mechanism for non-vernalization flowering of cabbage by grafting on radish stocks

    Motoki K, Doi M, Hosokawa M

    30th International Horticultural Congress（IHC2018）  2018.8 

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    Event date： 2018.8.12 - 2018.8.16

    Language：English   Presentation type：Poster presentation  

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  • The analysis of flowering characteristics and expression of genes related to flowering of non-flowering cabbage mutant.

    Kinoshita Y, Motoki K, Doi M, Hosokawa M

    Annual Meeting of the Korean Society for Plant Biotechnology  2018.6.1 

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    Event date： 2018.5.31 - 2018.6.2

    Language：English   Presentation type：Poster presentation  

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  • “接ぎ木”を使って花を咲かせる〜その分子基盤とアブラナ科作物の育種・遺伝資源保存への応用〜 Invited

    元木 航

    第 8 回 技能習得と発達についての人類学的研究研究会  2023.10.27 

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  • Development of a non-vernalization-grafting floral induction method for rapid breeding of Brassica crops

    Ko Motoki

    2023.8.27 

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    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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  • ダイコンのフロリゲンを利用したアブラナ科作物の早期開花技術の開発 Invited

    元木 航

    第18回けいはんな地区植物科学懇話会  2022.11.12 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • ダイコンがもつ花咲かホルモンの解析 ~植物育種に利用可能な“花成誘導台木”の開発に向けて~ Invited

    元木 航

    けいはんな学研都市7大学連携 市民公開講座2022  2022.9.16 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • ダイコンのフロリゲンを利用したキャベツの早期開花技術の開発 Invited

    元木 航

    第44回 岩手育種談話会・第27回植物生命科学セミナー  2022.9.15 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • なぜダイコンはキャベツを開花させるのか？〜植物育種に利用可能な"花成誘導台木"の開発に向けて〜 Invited

    元木 航

    京都大学農場オープンファーム・第25回 附属農場公開講座  2021.11.3 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • トマトの単為結果性遺伝⼦ pat-kによる高糖度化の機構解明に向けたRNA-seq解析

    福留千映, 滝澤理仁, 永野 惇, 元木 航, 山崎 彬, 西村和紗, 間合絵里, 中野龍平, 中﨑鉄也

    園芸学会令和3年度秋季大会  2021.9.11 

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  • ‘紅博桃’の新規枝変わりの第４染色体に検出された大規模なヘテロ接合性の消失

    谷口裕起, 牛島幸一郎, 河井 崇, 田村尚之, 福田文夫, 髙田大輔, 佐藤 守, 西村和紗, 間合絵里, 元木 航, 山崎 彬, 中﨑鉄也, 中野龍平

    園芸学会令和3年度秋季大会  2021.9.11 

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  • 単為結果性ニホンカボチャ‘OM1-P’の果実形成に関するオミックス解析

    小川萌, 滝澤理仁, 永野惇, 小嶋美紀子, 竹林裕美子, 榊原均, 元木航, 間合絵里, 西村和紗, 山崎彬, 中野龍平, 中﨑鉄也

    園芸学会令和 3 年度春季大会  2021.3 

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  • トマトのモデル品種‘Micro-Tom’の果実重を制御するQTLの機能解明と候補遺伝子の探索

    西田 敦, 滝澤理仁, 西村和紗, 間合絵里, 元木 航, 山崎 彬, 中野龍平, 中﨑鉄也

    園芸学会令和３年春季大会  2021.3 

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  • トマトの単為結果性遺伝⼦pat-kによる高糖度化の機構解明に向けたメタボローム解析

    福留千映, 滝澤理仁, 草野都, 小林誠, 斎藤和季, 間合絵里, 元木航, 西村和紗, 山崎彬, 中野龍平, 中﨑鉄也

    園芸学会令和 3 年度春季大会  2021.3 

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  • A candidate mechanism controlling floral repression in hardly flowering cabbage mutant

    Kinoshita Y, Motoki K, Hosokawa M

    3rd Asian Horticultural Congress (AHC2020)  2020.12 

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  • イネ品種台中65号の出穂日はBVPにおける日長条件によって変化する

    安部 学, 小梶裕之, 齋藤大樹, 西村和紗, 清水顕史, 中川博視, 矢部志央理, 中野龍平, 間合絵里, 元木 航, 永野 惇, 中﨑鉄也

    日本育種学会第138回講演会  2020.9 

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  • トマトの単為結果性遺伝子pat-kが果実内の糖代謝へ及ぼす影響

    福留千映, 滝澤理仁, 間合絵里, 元木 航, 西村和紗, 中野龍平, 中崎鉄也

    日本育種学会第138回講演会  2020.9 

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  • 難開花性キャベツ変異体‘不抽苔’においてFT遺伝子は機能を保持している

    木下有羽, 元木航, 白澤健太, 細川宗孝

    令和2年度園芸学会春季大会  2020.3.21 

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  • トマトの単為結果性変異体‘No.14’における単為結果と種子形成に関する特性評価

    北川直也, 滝澤理仁, 間合絵里, 西村和紗, 元木航, 中野龍平, 中﨑鉄也

    園芸学会令和2年春季大会  2020.3 

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  • 多作期移行試験による感光性が異なるイネ4品種の基本栄養成長期間の評価

    安部 学, 小梶裕之, 齋藤大樹, 西村和紗, 中川博視, 矢部志央理, 中野龍平, 滝澤理仁, 間合絵里, 元木 航, 中﨑鉄也

    育種学会第137回講演会  2020.3 

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Awards

  • 園芸学会年間優秀論文賞

    2024.3   一般社団法人 園芸学会   Investigation of the Field Performance of Progenies Obtained by a Non-vernalizationgrafting Floral Induction Method in Terms of Application to Cabbage Breeding and Seed Production.

    元木 航, 木下有羽, 中野龍平, 細川宗孝, 中﨑鉄也

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  • 園芸学会優秀発表賞（ポスター発表部門）

    2022.9   一般社団法人 園芸学会   FTタンパク質の定量解析によるキャベツの接ぎ木花成誘導条件の解明

    元木 航

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  • 優秀ポスター賞

    2021.6   超分野植物科学研究会   育種・採種のツールとして利用可能な"花成誘導台木"の開発

    元木 航

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  • Young Minds Award

    2018.8   A candidate mechanism for non-vernalization flowering of cabbage by grafting on radish stocks

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  • 若手研究者コンペティション 最優秀賞

    2017.9   園芸学会近畿支部   ダイコンへの接ぎ木により低温非遭遇で開花したキャベツの花成関連遺伝子の発現解析

    元木 航

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Research Projects

  • 接ぎ木による開花誘導において穂木の葉が開花を抑制するメカニズムの解明

    2024.09 - 2026.09

    公益財団法人両備てい園記念財団  研究助成金 

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    Authorship：Principal investigator 

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  • 根こぶ病抵抗性を付与した岩手県ブランドキャベツ品種「CR 春さやか」のスピードブリーディング

    2024.07 - 2029.03

    農業・食品産業技術総合研究機構，生物系特定産業技術研究支援センター  オープンイノべーション研究・実用化推進事業（開発研究ステージ，現場課題解決タイプ） 

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    Authorship：Coinvestigator(s) 

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  • マルチオミクス解析による接木を介した開花誘導メカニズムの解明と新規育種法の開発

    Grant number：24K21873  2024.06 - 2027.03

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

    門田 有希, 元木 航, 田中 勝

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    Grant amount：\6370000 （ Direct expense: \4900000 、 Indirect expense：\1470000 ）

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  • アブラナ科作物におけるフロリゲン遺伝子の機能解析

    Grant number：2419  2024.04 - 2025.03

    筑波大学つくば機能植物イノベーション研究センター  R６年度形質転換植物デザイン研究拠点共同利用・共同研究公募 

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  • キャベツ茎頂のフロリゲンへの応答性差異を生み出す遺伝子の同定

    Grant number：2024-4088  2024.04 - 2025.03

    公益財団法人 日本科学協会  笹川科学研究助成 

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    Authorship：Principal investigator 

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  • ダイコンの花成ホルモン高発現能に寄与するゲノム多型の同定・整理と新規素材の作出

    Grant number：23K13943  2023.04 - 2026.03

    日本学術振興会  科学研究費助成事業  若手研究

    元木 航

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    Grant amount：\4550000 （ Direct expense: \3500000 、 Indirect expense：\1050000 ）

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  • キャベツの強力な花成抑制機構を突破するダイコンの花成誘導因子の特定と採種への応用

    Grant number：20K15518  2020.04 - 2023.03

    日本学術振興会  科学研究費助成事業  若手研究

    元木 航

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    Grant amount：\4290000 （ Direct expense: \3300000 、 Indirect expense：\990000 ）

    本研究では、ダイコンへの接ぎ木による花成誘導法を利用した“花の咲かない野菜種子”生産体系の開発に向けて、ダイコンが示す強力な接ぎ木花成誘導能力に関わる因子の特定と、接ぎ木による採種法と通常の採種法とで得た種子の農業形質の比較を行う。本年度は接ぎ木したキャベツ穂木に蓄積したFTタンパク質を、昨年度に開発した抗FT抗体を用いて定量的に検出する手法の開発に取り組んだ。ダイコンに接ぎ木したキャベツの複数の組織からタンパク質を抽出し、抗FT抗体を用いたウエスタンブロット解析を行なった。その結果、上位葉の葉柄を用いることにより非特異的なバックグラウンドシグナルを抑えて、FTのバンドを検出することができた。さらに接ぎ木花成誘導能力に差がある2つのダイコン系統に接ぎ木したFTの蓄積量を比較したところ、系統間で違いが見られた。このことからダイコン系統間の接ぎ木花成誘導能力の違いはFTの供給能力の違いで説明できる可能性が示唆された。現在はこの仮説を検証するため、接ぎ木に用いるダイコン系統や、接ぎ木条件の違いによって生じるキャベツ穂木の開花反応の量的な違いが、FTの蓄積量から説明できるのかを調査している。同時に、接ぎ木花成誘導能力に差がある2つのダイコン系統の交雑F2集団を作出し、FT遺伝子発現量に関する遺伝解析を実施する準備を進めている。また採種法の異なるキャベツの種子の2年目の圃場試験を実施し、いずれの農業形質にも採種法による違いはほとんど認められないことを確認した。したがって、ダイコンへの接ぎ木による採種法は通常の採種法と同様に育種に利用できると考えられた。

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