Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.ajoms.2023.01.005

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Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND: We investigated and compared the osteogenic potential and bone regeneration capacities of dedifferentiated fat cells (DFAT cells) and adipose-derived stem cells (ASCs). METHOD: We isolated DFAT cells and ASCs from GFP mice. DFAT cells were established by a new culture method using a mesh culture instead of a ceiling culture. The isolated DFAT cells and ASCs were incubated in osteogenic medium, then alizarin red staining, alkaline phosphatase (ALP) assays, and RT-PCR (for RUNX2, osteopontin, DLX5, osterix, and osteocalcin) were performed to evaluate the osteoblastic differentiation ability of both cell types in vitro. In vivo, the DFAT cells and ASCs were incubated in osteogenic medium for four weeks and seeded on collagen composite scaffolds, then implanted subcutaneously into the backs of mice. We then performed hematoxylin and eosin staining and immunostaining for GFP and osteocalcin. RESULTS: The alizarin red-stained areas in DFAT cells showed weak calcification ability at two weeks, but high calcification ability at three weeks, similar to ASCs. The ALP levels of ASCs increased earlier than in DFAT cells and showed a significant difference (p < 0.05) at 6 and 9 days. The ALP levels of DFATs were higher than those of ASCs after 12 days. The expression levels of osteoblast marker genes (osterix and osteocalcin) of DFAT cells and ASCs were higher after osteogenic differentiation culture. CONCLUSION: DFAT cells are easily isolated from a small amount of adipose tissue and are readily expanded with high purity; thus, DFAT cells are applicable to many tissue-engineering strategies and cell-based therapies.

DOI： 10.3390/ijms222212392

PubMed

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Language：English   Publishing type：Research paper (scientific journal)  

Cathepsin K inhibitors are new drugs with the potential for the treatment of osteoporosis because they sustain bony remodelling better than bone resorption inhibitors such as bisphosphonates. The treatment of osteoporosis with inhibitors of bony resorption is associated with osteonecrosis of the jaw, as the deterioration in bony quality that they induce is thought to be one of its causes. The quality of bone is delineated by structural and material characteristics (which include the degree and quality of mineralisation, and depends on the content of proteoglycan and the structural integrity of the bony collagen).1,2 Animal and clinical studies have shown that cathepsin K inhibitors improve the mineral density and structural characteristics of bone, but their effect on the rest remains unknown. We therefore hypothesised that these inhibitors will affect the material characteristics of newly-formed mandibular bone. To verify our hypothesis, we used Raman microspectroscopy to examine such bone in rats that were given a cathepsin K inhibitor, and found unusual crystallinity and an increased substitution of carbonate (CO32-) in its crystal structure.

DOI： 10.1016/j.bjoms.2018.08.003

PubMed

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Language：English   Publishing type：Research paper (scientific journal)  

Bone quality is defined by structural and material characteristics. Most studies on the mandible have focused on the analysis of structural characteristics, with insufficient investigation of material characteristics. This study tested whether zoledronate affects the material characteristics of newly formed mandibular bone. Thirty-six female Wistar rats were assigned to three groups: sham-ovariectomized rats (SHAM, n=12), ovariectomized rats (OVX, n=12), and ovariectomized rats treated with zoledronate (ZOL, n=12). The left side of the mandibular ramus of all rats was drilled bicortically. Twenty-eight days after surgery, all surviving rats were euthanized and all mandibles were removed. Raman microspectroscopy was performed, and five spectra per specimen of newly formed mandibular bone were analysed. Compared with OVX rats, the mineral/matrix ratio in ZOL rats was significantly increased (5.43±1.88 vs. 7.86±2.05), while crystallinity (0.055±0.002 vs. 0.050±0.002), relative proteoglycan content (0.43±0.10 vs. 0.31±0.05), and collagen structural integrity (1.16±0.21 vs. 0.72±0.06) were significantly decreased. These changes in material characteristics may explain why rats that received zoledronate exhibited peculiar biological phenomena such as bisphosphonate-related osteonecrosis of the jaw.

DOI： 10.1016/j.ijom.2018.02.002

PubMed

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Language：Japanese   Publisher：岡山歯学会  

骨治癒過程におけるビスフォスフォネート製剤(BP)のTリンパ球分画への影響について検討した。12週齢C57BL/6J雌性Wildマウスを、生理食塩水を腹腔内投与したcontrol群とBPのひとつであるゾレドロネートを投与したzol群に分け、各群ともに12週間投与した。胸腺細胞では、Control群と比較してzol群はCD4シングルポジティブ(SP)細胞、CD8SP細胞、CD4CD8ダブルネガティブ(DN)細胞、γδT細胞で増加傾向を認め、CD4CD8ダブルポジティブ(DP)細胞で有意な減少を認めた。脾臓細胞では、control群と比較してzol群ではCD4SP細胞、DN細胞、γδT細胞で増加傾向を認め、CD8SP細胞、DP細胞で有意な減少を認めた。末梢血において、control群と比較してzol群はCD4SP細胞で増加傾向を認めた。BP投与が骨治癒過程におけるTリンパ球分画およびその関連因子である血清サイトカインに与える影響が明らかとなった。

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Language：Japanese   Publisher：(NPO)日本顎変形症学会  

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Language：Japanese   Publisher：(NPO)日本顎変形症学会  

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Language：Japanese   Publisher：(一社)日本口腔ケア学会  

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J-GLOBAL

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Language：Japanese   Publisher：(NPO)日本口腔科学会  

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Event date： 2023.11.10 - 2023.11.12

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2023.11.10 - 2023.11.12

Language：Japanese  

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Event date： 2023.6.8 - 2023.6.9

Language：Japanese   Presentation type：Poster presentation  

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Event date： 2023.6.8 - 2023.6.9

Language：Japanese   Presentation type：Poster presentation  

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Event date： 2023.4.20 - 2023.4.21

Language：English   Presentation type：Oral presentation (general)  

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