Faculty Profiles - MIMURA Yukari

MISC - MIMURA Yukari

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フレーベル『人間の教育』に見る「身体」と保育内容「健康」領域との関連性(第3報)「第二篇幼児期の人間(後編)」を中心として—Relevance between "body" and childcare details, "health" field shown in "human education" by Frobel(3rd report)Focusing on "chapter 2 human in early childhood(second part)"

髙橋敏之, 馬場訓子, 三村由香里, 津島愛子, 横田咲樹

日本幼少児健康教育学会誌 = Japanese journal of health education for children / 日本幼少児健康教育学会紀要編集委員会編 6 ( 2 ) 127 - 137 2021

Results of Musculoskeletal Examinations in Elementary School Students and its Challenges

( 164 ) 41 - 47 2017

BMI and body dissatisfaction values in Japanese male and female university students:—Difference in body images according to shilhouette chart—

KUNIMOTO Ayumi, YAMASAKI Sakiya, OKAMOTO Hiroshi, ISHII Nobuko, TAGO Takahito, TUTIOKA Daisuke, FUKUDA Takashi, HAYASHI Kyosuke, OGURA Toshio, TOJO Mitsuhiko, MIMURA Yukari, KIKUNAGA Shigeshi, MATSUEDA Mutsumi, KAMIMURA Hiroko, TSUSHIMA Aiko, KAGA Masaru, SAKO Haruko, DOI Mayu, SUZUKI Hisao, OKAZAKI Kanzou, AMALEO Katsuhiro, SAGAWA Kazunori, SHINTAKU Yukinori, TSUMIYAMA Takatsune, INOUE Yumiko, NARIYAMA Kouichi

Japanese Journal of Health Education and Promotion 25 ( 2 ) 74 - 84 2017

Language：Japanese Publisher：JAPANESE SOCIETY OF HEALTH EDUCATION AND PROMOTION

Objective: We conducted this study to examine the relationships among five ranges of BMI and two types of body dissatisfaction in male and female university students in Japan.<br>Method: We performed a cross sectional study using body composition measurement and questionnaire on body images among Japanese students (4,118 men and 2,677 women), 18 to 22 years old from 14 universities.<br>Results: The significant difference was detected in all five BMI ranges of female students between the degree of ideal dissatisfaction (ideal shape - current shape) and healthy dissatisfaction (healthy shape - current shape) (p＜0.001). Among men, however, such a significant difference was not found. In addition, the significant difference was not found in the choice of an attractive man between men and women. Conversely, the average silhouette value of the attractive female shape for men was 4.65, and the silhouette value of the attractive female shape for men from the viewpoint of women was 3.97. The female students showed significantly lower body image score than male students (t＝25.08, p＜0.001, d＝0.70).<br>Conclusion: For female college students, a healthy looking shape was a more plump woman than an ideal shape. As for the attractive shape of men, the difference was not detected between the views from both male and female students. However, for that of women, a slim woman was considered more attractive.

DOI： 10.11260/kenkokyoiku.25.74

異所性ACTH症候群を呈した胸腺発生大細胞神経内分泌癌の一例

当真貴志雄, 稲垣兼一, 小松原基志, 原孝行, 寺坂友博, 細谷武史, 越智可奈子, 山内尚子, 三村由香里, 和田淳, 大塚文男, 槇野博史

日本内分泌学会雑誌 90 ( 3 ) 915 - 915 2014.10

甲状腺乳頭癌を合併した多発性内分泌腫瘍症(MEN)1型の一例

越智可奈子, 稲垣兼一, 小松原基志, 原孝行, 寺坂友博, 細谷武史, 当真貴志雄, 山内尚子, 三村由香里, 小倉俊郎, 和田淳, 大塚文男, 槇野博史

日本内分泌学会雑誌 90 ( 3 ) 908 - 908 2014.10

Evaluation of Physical Assessment Training Using a Simulated Case for Yogo teacheres

9 ( 1 ) 31 - 46 2014

PRL分泌に対するBMP-4とメラトニン作用の影響:GH3細胞を用いた検討

越智可奈子, 大塚文男, 当真貴志雄, 中村絵里, 塚本尚子, 稲垣兼一, 三村由香里, 小倉俊郎, 岩崎泰正, 槇野博史

日本内分泌学会雑誌 90 ( 1 ) 2014

オーストラリア連邦のスクールナースの役割ニューサウスウェールズ州における調査から

山内愛, 松枝睦美, 加納亜紀, 三村由香里, 高橋香代

学校保健研究 55 ( 5 ) 425 - 435 2013.12

Language：Japanese Publisher：(一社)日本学校保健学会

わが国の養護教諭に相当するスクールナース(SN)の、オーストラリア連邦・ニューサウスウェールズ(NSW)州の私立学校における役割を明らかにし、A県の私立学校の養護教諭と比較することを目的にアンケート調査を行い、NS44名、養護教諭22名より回答を得た。また、NS2名を対象に面談調査を行った。その結果、SNが高く意識していた職務内容は「救急処置」と「保健室経営」で、NSW州では学校保健の職務はSNの他、スクールカウンセラー、保健機関、委託業者、保護者などが関わり学校内外で役割分担がなされており、SNは子どもの健康課題の直接的ケアを行う看護職としての役割を担っていることが分かった。一方、養護教諭が高く意識していた職務内容は、「救急処置」と「保健室経営」の他、「保健教育」「健康相談」などで、学校保健に関する幅広い役割を担っていた。

Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2013&ichushi_jid=J00253&link_issn=&doc_id=20140108490003&doc_link_id=%2Fej6gahok%2F2013%2F005505%2F006%2F0425-0435%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fej6gahok%2F2013%2F005505%2F006%2F0425-0435%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

若年者の甲状腺疾患大学新入生健康診断によるスクリーニング

小倉俊郎, 岩崎良章, 古賀光, 三村由香里, 稲垣兼一, 三好智子, 塚本尚子, 大塚文男

日本内分泌学会雑誌 89 ( 2 ) 479 - 479 2013.9

FDG-PETで集積を認めたACTH産生性下垂体腺腫の1例

越智可奈子, 大塚文男, 細谷武史, 原孝行, 当真貴志雄, 中村絵里, 塚本尚子, 稲垣兼一, 三好智子, 三村由香里, 小倉俊郎, 山田正三, 槇野博史

日本内分泌学会雑誌 89 ( 3 ) 2013

左眼瞼下垂を契機に診断された若年胃癌のトルコ鞍部転移の一例

稲垣兼一, 当真貴志雄, 中村絵里, 越智可奈子, 塚本尚子, 三好智子, 三村由香里, 小倉俊郎, 大塚文男, 槇野博史

ACTH Related Peptides 24 2013

Rapid progression of Graves' ophthalmopathy despite the administration of thiamazole.

Katsuyama T, Takeda M, Otsuka F, Toma K, Inagaki K, Sada KE, Mimura Y, Ogura T, Makino H

Intern Med 52 ( 20 ) 2317 - 2320 2013

Erdheim-Chester病が強く疑われた下垂体機能低下症の一例

三好智子, 大塚文男, 稲垣兼一, 田中康司, 越智可奈子, 藤井一恭, 藤井伸治, 当真貴志雄, 中村絵里, 塚本尚子, 武田昌也, 三村由香里, 小倉俊郎, 景山甚郷, 槇野博史

日本内分泌学会雑誌 88 ( 3 ) 1017 - 1017 2012.12

KisspeptinによるGnRH発現調節とBMP/Estrogenの関与

寺坂友博, 大塚文男, 稲垣兼一, 三好智子, 中村絵里, 塚本尚子, 武田昌也, 越智可奈子, 三村由香里, 小倉俊郎, 槇野博史

日本内分泌学会雑誌 88 ( 1 ) 267 - 267 2012.4

Assessment Checklist for Seriously Injured Arms and Legs by Yogo Teacher's First Aid at School : A trial clarifies present conditions and problems

15 ( 2 ) 13 - 22 2012

オクトレオチドが著効した急性発症・原因不明の異所性ＡＣＴＨ症候群の１例

稲垣兼一, 大塚文男, 当真貴志雄, 越智可奈子, 中村絵里, 塚本尚子, 武田昌也, 三好智子, 三村由香里, 小倉俊郎, 槇野博史

ACTH RELATED PEPTIDES 23 58 - 60 2012

重度の吸収障害でLT４投与法に苦慮した甲状腺機能低下症の一例.

越智可奈子, 大塚文男, 中村絵里, 塚本尚子, 武田昌也, 稲垣兼一, 三好智子, 三村由香里, 小倉俊郎, 名和秀起, 槇野博史

日本甲状腺学会雑誌特集１ホルモン作用：作用機序と破綻による疾患 3 ( 1 ) 41 - 45 2012

反復する低血糖より発見された高齢発症の膵Nesidioblastosisの一例

武田昌也, 大塚文男, 当真貴志雄, 越智可奈子, 中村絵里, 塚本尚子, 稲垣兼一, 三村由香里, 小倉俊郎, 景山甚郷, 槇野博史

日本内分泌学会雑誌 88 ( 1 ) 2012

局在不明の腫瘍性低リン血症性骨軟化症の一例

細谷武史, 稲垣兼一, 益田加奈, 越智可奈子, 中村絵里, 塚本尚子, 武田昌也, 三好智子, 三村由香里, 小倉俊郎, 景山甚郷, 大塚文男, 槇野博史

日本内分泌学会雑誌 88 ( 3 ) 2012

ペグビソマント少量間欠投与にて肝障害を認めた先端巨大症の1例

稲垣兼一, 大塚文男, 当真貴志雄, 越智可奈子, 中村絵里, 塚本尚子, 三好智子, 三村由香里, 小倉俊郎, 景山甚郷, 山田正三, 槇野博史

日本内分泌学会雑誌 88 ( 3 ) 2012

筋強直性ジストロフィー合併原発性副甲状腺機能亢進症の一例

塚本尚子, 稲垣兼一, 当真貴志雄, 越智可奈子, 中村絵里, 三好智子, 三村由香里, 小倉俊郎, 景山甚郷, 大塚文男, 槇野博史

日本内分泌学会雑誌 88 ( 3 ) 2012

頭痛と脳神経症状にて発見された若年胃癌による下垂体転移の一例

中村絵里, 稲垣兼一, 当真貴志雄, 越智可奈子, 塚本尚子, 三好智子, 三村由香里, 小倉俊郎, 景山甚郷, 大塚文男, 槇野博史

日本内分泌学会雑誌 88 ( 3 ) 2012

術後肝障害を呈したCushing症候群3症例の検討

当真貴志雄, 大塚文男, 越智可奈子, 中村絵里, 塚本尚子, 武田昌也, 三好智子, 稲垣兼一, 三村由香里, 小倉俊郎, 影山甚郷, 槇野博史

日本内分泌学会雑誌 88 ( 3 ) 2012

GT1-7細胞におけるBMPとEstrogenによるGnRH分泌制御とKisspeptinの関与

寺坂友博, 大塚文男, 稲垣兼一, 三好智子, 中村絵里, 塚本尚子, 武田昌也, 三村由香里, 小倉俊郎, 槇野博史

日本内分泌学会雑誌 87 ( 1 ) 332 - 332 2011.4

抗甲状腺薬により劇症肝炎をきたし肝移植にて救命しえた一例

塚本尚子, 大塚文男, 鈴木二郎, 武田昌也, 稲垣兼一, 小倉可奈子, 中村絵里, 三好智子, 三村由香里, 小倉俊郎, 元木崇之, 槇野博史

日本内分泌学会雑誌 87 ( Suppl. ) 35 - 37 2011

Language：Japanese Publisher：(一社)日本内分泌学会

26歳女。動悸を契機にバセドウ病と診断され、チアマゾールで治療を開始したが、皮疹が出現したために内服を自己中断した。プロピルチオウラシル(PTU)に変更して、甲状腺機能の正常化を認めたが、肝機能障害が出現し、PTUを減量した。著明な肝機能障害の増悪を指摘され、PTUを中止し無機ヨード内服に変更となり、紹介入院となった。劇症肝炎に対しステロイドパルス、FFP輸注及びCHDFを行うも、肝萎縮の進行と肝性脳症が出現し、保存的加療での救命は困難と判断した。同胞(兄)をドナーとして、準緊急的生体部分肝移植を施行した。摘出肝の組織検査所見では、劇症肝炎に合致する所見であった。甲状腺は、移植後第6病日に甲状腺全摘出術を施行した。その後は肝機能も安定し、レボチロキシン補充を行い外来通院にて経過観察となった。移植肝は拒絶反応なく生着したことがCTにて確認した。

Development of case material and evaluation of case-method education for Yogo teacher students

Journal of Japan Association of Universities of Education 29 249 - 262 2011

養護教諭養成教育における「教職実践ポートフォリオ」の開発ー岡山大学の取り組みー

上村弘子, 住野好久, 松枝睦美, 三村由香里, 田嶋八千代, 伊藤武彦, 門田新一郎, 太田泰子, 本田浩江, 番場和恵, 村田亜矢子, 高橋香代

日本教育大学協会研究年報 29 73 - 90 2011

First Aid Performed by Yogo Teachers : Judgment and Response in Practice

53 ( 5 ) 399 - 410 2011

急性膵炎を合併し生検にてIgG4関連下垂体炎と診断し得た1例

越智可奈子, 大塚文男, 中村絵里, 塚本尚子, 武田昌也, 稲垣兼一, 三好智子, 三村由香里, 小倉俊郎, 石田穣治, 小野成紀, 伊達勲, 槇野博史

日本内分泌学会雑誌 87 ( 3 ) 2011

海綿静脈洞サンプリングにて同定しえたクッシング病の一例

三好智子, 大塚文男, 当真貴志雄, 越智可奈子, 中村絵里, 塚本尚子, 武田昌也, 稲垣兼一, 三村由香里, 小倉俊郎, 景山甚郷, 槇野博史, 黒住和彦, 小野成紀, 伊達勲

日本内分泌学会雑誌 87 ( 3 ) 2011

慢性イレウス・吸収不良により経口T₄製剤不応性の甲状腺機能低下症の1例

越智可奈子, 大塚文男, 中村絵里, 塚本尚子, 武田昌也, 稲垣兼一, 三好智子, 三村由香里, 小倉俊郎, 名和秀起, 槇野博史

日本内分泌学会雑誌 87 ( 2 ) 2011

重度の吸収障害でLT4投与法に苦慮した甲状腺機能低下症の1例

越智可奈子, 大塚文男, 中村絵里, 塚本尚子, 武田昌也, 稲垣兼一, 三好智子, 三村由香里, 小倉俊郎, 名和秀起, 槇野博史

日本内分泌学会雑誌 87 ( 3 ) 2011

血栓性血小板減少性紫斑病を生じたクッシング症候群の1例.

大塚文男, 瀧上慶一, 氏家はる代, 稲垣兼一, 三好智子, 武田昌也, 塚本尚子, 中村絵里, 三村由香里, 小倉俊郎, 槇野博史

ACTH Related Peptides 22 80 - 81 2011

35年前の外傷による視床下部・下垂体機能低下症と診断した一例

稲垣兼一, 山成俊夫, 難波早耶香, 越智可奈子, 中村絵里, 塚本尚子, 武田昌也, 三好智子, 大塚文男, 三村由香里, 槇野博史

日本内分泌学会雑誌 87 ( 3 ) 2011

妊娠・出産の経過中に検出されたマクロプロラクチン血症の一例

塚本尚子, 大塚文男, 服部尚樹, 鎌田泰彦, 越智可奈子, 中村絵里, 武田昌也, 三好智子, 稲垣兼一, 三村由香里, 小倉俊郎, 槇野博史

日本内分泌学会雑誌 87 ( 3 ) 2011

同時摘出した副甲状腺機能亢進症とパラガングリオーマの合併例

安田麻衣子, 稲垣兼一, 大塚文男, 小倉可奈子, 武田昌也, 塚本尚子, 中村絵里, 三好智子, 鈴木二郎, 三村由香里, 小倉俊郎, 槇野博史

日本内分泌学会雑誌 86 ( 3 ) 745 - 745 2010.12

Effects of fibroblast growth factor (FGF)-8 on ovarian steroidogenesis: involvement of upregulation of BMP receptor singnaling

Tomoko Miyoshi, Fumio Otsuka, Misuzu Yamashita, Kenichi Inagaki, Jiro Suzuki, Eri Nakamura, Naoko Tsukamoto, Masaya Takeda, Yukari Mimura, Toshio Ogura, Hirofumi Makino

ENDOCRINE JOURNAL 57 S613 - S614 2010.3

Enhancement of aldosterone-induced catecholamine production by bone morphogenetic protein-4 through activating Rho and SAPK/JNK pathway in adrenomedullar cells

Junko Goto, Fumio Otsuka, Misuzu Yamashita, Jiro Suzuki, Hiroyuki Otani, Hiroko Takahashi, Tomoko Miyoshi, Yukari Mimura, Toshio Ogura, Hirofumi Makino

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 296 ( 4 ) E904 - E916 2009.4

Language：English Publisher：AMER PHYSIOLOGICAL SOC

Goto J, Otsuka F, Yamashita M, Suzuki J, Otani H, Takahashi H, Miyoshi T, Mimura Y, Ogura T, Makino H. Enhancement of aldosterone-induced catecholamine production by bone morphogenetic protein-4 through activating Rho and SAPK/JNK pathway in adrenomedullar cells. Am J Physiol Endocrinol Metab 296: E904-E916, 2009. First published February 9, 2009; doi:10.1152/ajpendo.90840.2008.-Here we investigated the effects of mineralocorticoid in the regulation of catecholamine biosynthesis using rat pheochromocytoma PC12 cells. Expression of mineralocorticoid receptor (MR) was confirmed in undifferentiated PC12 cells. Aldosterone stimulated dopamine production by PC12 cells without any increase in cAMP activity. Aldosterone-induced dopamine accumulation was enhanced in accordance with the increase in the rate-limiting enzyme tyrosine hydroxylase (TH). Blocking MR with eplerenone suppressed aldosterone-induced increases of TH mRNA and dopamine production. A glucocorticoid receptor (GR) antagonist, RU-486, attenuated dexamethasone-but not aldosterone-induced TH expression. Cycloheximide reduced both aldosterone-and dexamethasone-induced TH mRNA. A SAPK/JNK inhibitor, SP600125, suppressed aldosterone-induced TH mRNA expression; however, the aldosterone-induced TH expression was not affected by inhibition of ERK1/2, p38-MAPK, Rho-kinase, PI 3-kinase, and PKC. It was of note that cotreatment with eplerenone and SP600125 restored aldosterone-induced TH mRNA expression to basal levels. To investigate the involvement of bone morphogenetic protein (BMP) actions in aldosterone-induced catecholamine production, we examined the effects of BMP-4 and BMP-7, which are expressed in the adrenal medulla, on catecholamine biosynthesis. BMP-4 preferentially enhanced aldosterone-induced TH mRNA and dopamine production, although BMP-4 alone did not affect TH expression. The BMP-4 enhancement of aldosterone-induced TH expression was not observed in cells treated with eplerenone. BMP-4 did not affect MR expression of PC12 cells; however, it did enhance aldosterone-induced SAPK/JNK phosphorylation. Inhibition of SAPK/JNK or Rho suppressed BMP-4 enhancement of aldosterone-induced TH expression. Collectively, our findings demonstrate that aldosterone stimulates catecholamine biosynthesis in adrenomedullar cells via MR through genomic action and partly through nongenomic action by Rho-SAPK/JNK signaling, the latter of which is facilitated by BMP-4. A functional link between MR actions and endogenous BMP may be involved in the catecholamine production.

DOI： 10.1152/ajpendo.90840.2008

A simple device to measure and record wet bulb globe temperature for prevention of heat disorders

Bulletin of Graduate School of Education, Okayama University ( 140 ) 7 - 11 2009

Usefulness and future problems of moist wound healing in school

Journal of Japanese Association of Yogo Teacher Education 12 ( 1 ) 105 - 111 2009

A simple device to measure and record wet bulb globe temperature for prevention of heat disorders.

Ito T, Mimura Y, Suzuki H

Bulletin of Graduate School of Education, Okayama University ( 140 ) 7 - 11 2009

Usefulness and future problems of moist wound healing in school

Mimura Y, Kamimura H, Kuroki K, Kinumi Y, Matsueda M, Ogura T, Takahashi K

Journal of Japanese Association of Yogo Teacher Education 12 ( 1 ) 105 - 111 2009

低Na血症を契機に発見された結核性リンパ節炎の１例

大谷寛之, 大塚文男, 塚本尚子, 中村絵里, 後藤順子, 三好智子, 武田昌也, 鈴木二郎, 三村由香里, 小倉俊郎, 槇野博史

日本内分泌学会雑誌 85 ( suppl ) 54 - 55 2009

4年の経過でレニン抑制が顕著となり、腺腫の摘出に至った原発性アルドステロン症の一例

塚本尚子, 大塚文男, 鈴木二郎, 三好智子, 大谷寛之, 後藤順子, 三村由香里, 小倉俊郎, 槇野博史

日本内分泌学会雑誌 83 ( 4 ) 1085 - 1085 2008.3

A rare tumor in the adrenal region: Neuron-specific enolase (NSE)-producing leiomyosarcoma in an elderly hypertensive patient

Junko Goto, Fumio Otsuka, Ryo Kodera, Tomoko Miyoshi, Masaru Kinomura, Hiroyuki Otani, Yukari Mimura, Toshio Ogura, Hiroyuki Yanai, Yasutomo Nasu, Hirofumi Makino

ENDOCRINE JOURNAL 55 ( 1 ) 175 - 181 2008.2

Language：English Publisher：JAPAN ENDOCRINE SOC

A 73-year-old Japanese woman was referred for examination of right flank pain and progressive hypertension. Abdominal CT incidentally detected a right adrenal mass 8 cm in size. The tumor exhibited isodensity by CT and contained high-intense lesion by T2-weighted MRI. Scintigraphy with I-131-metaiodobenzylguanidine and I-131-adosterol showed no abnormal uptake by whole body scan. Positron emission tomography scan with F-18-2-fluoro-D-deoxyglucose demonstrated an exclusive uptake in the right adrenal mass. Adrenocortical hormone levels and catecholamine secretion were within normal range; however, the level of serum neuron-specific enolase (NSE) was found to be markedly high. After controlling systemic blood pressure with an alpha 1-blocker, the right adrenal tumor was surgically removed, along with the right kidney and inferior vena cava which adhered to it. The tumor was pathologically proven to be leiomyosarcoma, which was immunohistochemically positive with a-smooth muscle actin and negative with CD57, S-100 and c-kit proteins. Notably, NSE protein was massively expressed in the resected tumor. After surgery blood pressure was controlled with regular medication and serum NSE levels have since normalized. The possibility of leiomyosarcoma should be kept in mind in adrenal incidentalomas with rapid growth and atypical radiological images. Our findings suggest that circulating NSE levels may be clinically useful for early detection of recurrence.

DOI： 10.1507/endocrj.K07E-020

甲状腺機能低下症により顕在化したApparent Mineralocorticoid Excwss(AME)病態の一例

稲垣兼一, 大塚文男, 鈴木二郎, 三好智子, 大谷寛之, 三村由香里, 小倉俊郎, 槇野博史

ホルモンと臨床 56 119 - 123 2008

Study on first aid performed by Yogo teachers in schools: Yogo teachers' duties suggested by precedents

Japanese journal of school health 50 ( 4 ) 221 - 233 2008

Checklist proposal for assessing head injury severity in Yogo practice

Journal of Japanese Association of Yogo Teacher Education 11 ( 1 ) 16 - 25 2008

養護教諭の救急処置における困難と今後の課題－記録と研修に着目して－

武田和子, 三村由香里, 松枝睦美, 河本妙子, 上村弘子, 高橋香代

日本養護教諭教育学会誌 11 ( 1 ) 33 - 43 2008

Effects of peroxisome proliferator-activated receptor activation on gonadotropin transcription and cell mitosis induced by bone morphogenetic proteins in mouse gonadotrope L beta T2 cells

Masaya Takeda, Fumio Otsuka, Hiroyuki Otani, Kenichi Inagaki, Tomoko Miyoshi, Jiro Suzuki, Yukari Mimura, Toshio Ogura, Hirofumi Makino

JOURNAL OF ENDOCRINOLOGY 194 ( 1 ) 87 - 99 2007.7

Language：English Publisher：SOC ENDOCRINOLOGY

Involvement of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) activation and bone morphogenetic protein (BMP) signaling in regulating cell proliferation and hormonal production of pituitary tumors has been reported, although the underlying mechanism remains poorly understood. Here, we investigated regulatory roles of PPAR alpha. and PPAR gamma in gonadotropin transcription and cell mitosis modulated by pituitary activin/BMP systems using a mouse gonadotropinoma cell line L beta T2, which expresses activin/BMP receptors, transcription factor Smads, PPAR alpha, and PFARy. In L beta T2 cells, BMP signaling shown by Smad 1/5/8 phosphorylation and Id-1 transcription was readily activated by BMPs. A PPAR gamma agonist, pioglitazone significantly reduced BMP-induced DNA synthesis by L beta T2; whereas the PPARa agonist, fenofibric acid, did not. In accordance with the effects on cell mitosis, pioglitazone but not fenofibric acid significantly decreased BMP-induced Id-1-Luc activation. Neither fenofibric acid nor pioglitazone affected activin signaling detected by (CAGA)(9)-Luc activity. Both PPAR alpha and PPAR gamma ligands directly suppressed transcriptional activities of FSH beta, LH beta, and GnRHR. Activation of PPARg. and PPAK gamma increased mRNA levels of follistatin, but did not affect the expression of follistatin-related gene. Thus, PPAR agonists not only directly suppress gonadotropin transcription and BMP signaling, but also inhibit the biological actions of activins which facilitate gonadotropin transcription through upregulating follistatin expression. In addition, pioglitazone increased BMP ligands mRNA, but decreased activin-beta B mRNA in L beta T2 cells. Collectively, PPAR activation differentially regulates gonadotrope cell proliferation and gonadotropin transcription in a ligand-dependent manner.

DOI： 10.1677/JOE-07-0138

リンパ球性漏斗神経葉炎と肺癌脳転移を伴った中枢性尿崩症の一例

大谷寛之, 大塚文男, 廣田大昌, 三好智子, 稲垣兼一, 鈴木二郎, 後藤順子, 三村由香里, 小倉俊郎, 田端雅弘, 槇野博史

日本内分泌学会雑誌 83suppl 208 - 210 2007

養護診断『心理的な要因が存在する可能性のある状態』の診断名と診断指標の開発

岡田加奈子, 葛西敦子, 三村由香里, 徳山美智子, 竹鼻ゆかり, 荒木田美香子, 荒川雅子, 井上智恵子, 齋藤理砂子, 西能代, 冨塚都仁子, 高田しずか, 外間朝子, 岩田美保, 山本雅

日本養護教諭教育学会誌 10 1 - 18 2007

MDSを合併した先端巨大症に対するオクトレオチドによる１治療例

三好智子, 大塚文男, 稲垣兼一, 鈴木二郎, 大谷寛之, 後藤順子, 三村由香里, 小倉俊郎, 景山甚郷, 前田嘉信, 槇野博史

日本内分泌学会雑誌 83suppl 79 - 81 2007

高血圧の精査にて発見されたNSE産生性副腎平滑筋肉腫の一例.

後藤順子, 大塚文男, 小寺亮, 稲垣兼一, 三好智子, 大谷寛之, 鈴木二郎, 三村由香里, 小倉俊郎, 那須保友, 柳井広之, 槇野博史

日本内分泌学会雑誌 83 242 - 245 2007

多嚢胞性卵巣症候群を合併した先端巨大症の一例.

大塚文男, 稲垣兼一, 鈴木二郎, 三好智子, 大谷寛之, 後藤順子, 三村由香里, 小倉俊郎, 槇野博史

第三回アクロメガリーフォーラム記録集 10 2007

Mutual regulation of follicle-stimulating hormone signaling and bone morphogenetic protein system in human granulosa cells

T Miyoshi, F Otsuka, Suzuki, I, M Takeda, K Inagaki, Y Kano, H Otani, Y Mimura, T Ogura, H Makino

BIOLOGY OF REPRODUCTION 74 ( 6 ) 1073 - 1082 2006.6

Language：English Publisher：SOC STUDY REPRODUCTION

Bone morphogenetic proteins (BMPs) play critical roles in folliculogenesis by modulating the actions of follicle-stimulating hormone (FSH) in the ovary. However, the effects of FSH on the BMP system remain unknown. Here, we have investigated the effects of FSH on BMP signaling using the human granulosa-like tumor cell line KGN. KGN cells express BMP type 1 and type II receptors and the BMP signaling molecules SMADs. FSH administration upregulated BMP type IA (BMPR1A) and IB (BMPR1B) receptors, activin type II receptor (ACVR2), and BMP type II receptor (BMPR2). FSH also augmented SMAD1 and SMAD5 expression, and conversely, FSH suppressed the expression of the inhibitory SMADs, SMAD6 and SMAD7. Bioassays revealed that FSH enhances BMP-induced SMAD1/5/8 phosphorylation and cellular DNA synthesis induced by BMP6 and BMP7. Since overexpression of BMPR1A and BMPR1B, but not SMADs, significantly enhanced the BMP responses, these type I receptors were revealed to be limiting factors for BMP signaling in KGN cells. BMPs significantly suppressed progesterone synthesis induced by forskolin and dibutyryl-cAMP (BtcAMP) but had no effect on estradiol induced by the same factors. KGN cAMP levels induced by forskolin were not altered by BMPs, suggesting that BMPs regulate steroidogenesis at a level downstream of cAMP synthesis in KGN cells. In this regard, BMPs specifically reduced the STAR transcription, whereas the levels of CYP11A, HSD3B2, and CYP19 stimulated by forskolin as well as BtcAMP were not altered. Collectively, the two major factors, FSH-cAMP pathway and BMP system, are reciprocally and functionally linked. Given that BMPs downregulate FSH receptors in KGN cells, this interaction may contribute to finetuning of the mutual sensitivity toward BMP ligands and FSH.

DOI： 10.1095/biolreprod.105.047969

Involvement of bone morphogenetic protein-6 in differential regulation of aldosterone production by angiotensin II and potassium in human adrenocortical cells

Kenichi Inagaki, Fumio Otsuka, Jiro Suzuki, Yoshihiro Kano, Masaya Takeda, Tomoko Miyoshi, Hiroyuki Otani, Yukari Mimura, Toshio Ogura, Hirofumi Makino

ENDOCRINOLOGY 147 ( 6 ) 2681 - 2689 2006.6

Language：English Publisher：ENDOCRINE SOC

Aldosterone production is modified by several growth factors that reside in the adrenal. We have recently reported the existence of a bone morphogenetic protein (BMP) system in human adrenocortical cells, in which BMP-6 augments aldosterone synthesis. Here, we investigated functional roles of BMP-\6, focusing on the differential regulation of aldosterone synthesis induced by angiotensin (Ang) II and potassium (K). In human adrenocortical H295R cells, BMP-6 augmented Ang II-induced CYP11B2 transcription and mRNA and aldosterone roduction but had no effect on K-induced aldosterone production. Inhibition of endogenous BMP-6 action by neutralizing antibodies impaired aldosterone production induced by Ang II but not that induced by K. Blockage of ligand-receptor binding using extracellular domain (ECD) of BMP type I receptors revealed that ECDs to activin receptor-like kinase (ALK)-2 and ALK-3 significantly reduced the aldosterone production induced by Ang II. None of the type I-receptor ECDs tested had any effect on K-induced aldosterone levels. Overexpression of a dominant negative-activin type II receptor construct selectively decreased Ang II-induced aldosterone production without having any effect on K-induced aldosterone production. BMP type II receptor-dominant negative had no effect on aldosterone induced by either Ang II or K. These results infer that BMP-6 acts through ALK-2, ALK-3, and activin type II receptor receptors in adrenocortical cells. BMP-6 pretreatment extends the induction of ERK1/2 phosphorylation by Ang II and treatment with ECDs to ALK-2 and ALK-3 impaired Ang II-induced ERK phosphorylation. The specific inhibitor of ERK activation, U0126, suppressed the activation of CYP11B2 transcription induced by BMP-6 without affecting Smad phosphorylation and Tlx2-Luc activity. Collectively, the endogenous BMP-6 system plays critical roles in aldosterone production between Ang II and K through ERK signaling pathway.

DOI： 10.1210/en.2005-1250

バセドウ病を合併した甲状腺不応症の１例

稲垣兼一, 大塚文男, 鈴木二郎, 三好智子, 武田昌也, 大谷寛之, 中村絵里, 三村由香里, 小倉俊郎, 槇野博史

日本内分泌学会雑誌 2006

Functional roles of the bone morphogenetic protein system in thyrotropin signaling in porcine thyroid cells

J Suziki, F Otsuka, M Takeda, K Inagaki, T Miyoshi, Y Mimura, T Ogura, H Doihara, H Makino

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 327 ( 4 ) 1124 - 1130 2005.2

Language：English Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE

We uncovered a new regulation of thyrocyte function by bone morphogenetic protein (BMP) under the influence of thyrotropin (TSH) using primary culture of porcine thyrocytes. The BMP type I receptors, ALK-2 (ActRIA), -3 (BMPRIA), and -6 (BMPRIB), were expressed in porcine thyrocytes, while ALK-6 was not detected in human thyroid. Treatment with BMP-2,-4,-6,-7, and TGF-beta1 exhibited a dose-dependent suppression of DNA synthesis by porcine thyrocytes. BMP-2, -4, -6, -7, and TGF-beta1 suppressed TSH receptor mRNA expression on thyrocytes, which was consistent with their suppressive effect on TSH-induced cAMP synthesis and TSH-induced insulin-like growth factor-1 expression. Activin exhibited minimal suppression of thyrocyte DNA synthesis and did not exhibit suppressive effects on TSH receptor mRNA expression. Phosphorylated Smad1/5/8 was detected in the lysates of porcine thyrocytes treated with BMP-2, -4,-6, and -7. However, in the presence of TSH, BMP-6 and -7 failed to activate Smad1/5/8 phosphorylation and 3TP-reporter activity, whereas BMP-2 and -4 maintained clear activation of the BMP signaling regardless of the presence of TSH. This diverged regulation of thyroid BMP system by TSH is most likely due to the reduction of ALK-6 expression caused by TSH. Thus, the thyroid BMP system is functionally linked to TSH actions through modulating TSH receptor expression and TSH, in turn, selectively inhibits BMP signaling. Given that BMP system is present in human thyroid and the expression pattern of ALK-2 and BMPRII is different between follicular adenomas and normal thyroid tissues, the endogenous BMP system may be involved in regulating thyrocyte growth and TSH sensitivity of human thyroid adenomas. (C) 2004 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2004.12.122

副腎偶発腫の経過観察中に両側副腎の腫大を生じ，PETにより原発巣を検出した転移性副腎腫瘍の一例

大塚文男, 三好智子, 鈴木二郎, 稲垣謙一, 武田昌也, 加納義浩, 岸田雅之, 三村由香里, 小倉俊郎, 景山甚郷, 橋本浩三, 槇野博史

ACTH RELATED PEPRIDES 16 223 - 227 2005

Language：Japanese Publisher：間脳・下垂体・副腎系研究会

56歳男.健診にて血糖異常を指摘されスクリーニングCTにて右副腎腫瘍を認め,精査目的で入院した.アドステロールシンチを含む検査結果より,非機能性副腎腺腫が疑われたため外来経過観察とした.6ヵ月後右腹部から右腰背部に鈍痛が出現し,腹部画像検査にて副腎腫瘍の増大を認め,腫瘍内出血を疑い入院した.副腎腫瘍はホルモン産生を欠き,両側性であること,非常に急速に増大していることから,副腎癌,転移,悪性リンパ腫などが考えられたが,ガリウムシンチ・骨シンチでリンパ節転移は否定された.開腹手術により両側性副腎摘出術を施行し,副腎組織に腺癌浸潤像を認めた.術後,FDG-PET検査にて左上肺野に有意な核種取り込みを認め,肺腺癌の両側副腎転移として化学療法を行ったが,肺のブラ所見に変化は認められなかった

小学生における調節誤差に関する検討－近視進行と読書距離に着目して－

宝諸昌世, 三村由香里, 大角博子, 長谷部聡, 高橋香代

学校保健研究 2005

中枢性尿崩症で発症し，汎下垂体機能低下を来したgerminomaの１例

稲垣兼一, 大塚文男, 松井利浩, 鈴木二郎, 武田昌也, 三好智子, 加納義浩, 岸田雅之, 三村由香里, 小倉俊郎, 市村浩一, 景山甚郷, 槇野博史

日本内分泌学会雑誌 2005

Aldosterone regulation for 18 years in a case of primary aldosteronism

T Miyoshi, F Otsuka, T Ogura, J Suzuki, K Inagaki, M Takeda, M Kishida, Y Mimura, A Nagai, H Makino

AMERICAN JOURNAL OF HYPERTENSION 17 ( 6 ) 546 - 548 2004.6

Recent trends of hyperuricemia and obesity in Japanese male adolescents, 1991 through 2002

T Ogura, K Matsuura, Y Matsumoto, Y Mimura, M Kishida, F Otsuka, K Tobe

METABOLISM-CLINICAL AND EXPERIMENTAL 53 ( 4 ) 448 - 453 2004.4

Language：English Publisher：W B SAUNDERS CO

The aim of the present study was to determine the change of serum uric acid (UA) levels in male adolescents and to characterize the relationship between UA levels and obesity or its related factors. This study was conducted in 17,155 students at enrollment in Okayama University from 1991 through 2002, in which the mean serum UA level as a whole was 5.64 +/- 0.009 mg/dL (mean +/- SEM) and the incidence of hyperuricemia (greater than or equal to7.6 mg/dL) was 4.13%. Serum UA levels were correlated with obesity-related indicators, including body mass index (BMI; r = 0.282, P < .0001) and skin-fold thickness (r = 0.286, P < .0001). The incidence of hyperuricemia was increased in parallel with BMI. In the last 4 years (1999 through 2002) of the study period, serum UA levels (5.76 mg/dL) and the incidence of hyperuricemia (4.5%) were significantly increased compared with those in the earlier period (1991 through 1994: 5.50 mg/dL and 3.5%, respectively). However, BMI has been rather gradually decreased throughout 12-year observation in all the subjects. Hyperuricemia was related to the presence of other risk factors, including hypercholesterolemia, liver function abnormality, and hypertension. The frequencies of such abnormalities were higher than euuricemic subjects and this trend was notable in the most recent students enrolled from 1999 through 2002. Hyperuricemia was even found in the group of non-obese male adolescents. Taking into consideration that hyperuricemia is associated with a high prevalence of lifestyle-related diseases in adults, it is of great importance to prevent hyperuricemia at the early stage in Japanese adolescents. (C) 2004 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.metabol.2003.11.017

妊娠中に顕在化した周期性クッシング病の一例

大塚文男, 三好智子, 鈴木二郎, 稲垣謙一, 武田昌也, 岸田雅之, 三村由香里, 小倉俊郎, 景山甚郷, 橋本浩三, 槇野博史

ACTH RELATED PEPRIDES 15 187 - 193 2004

Language：Japanese Publisher：間脳・下垂体・副腎系研究会

19歳女.妊娠後33週より高血圧・蛋白尿が出現し,35週には浮腫と体重増加を伴う妊娠中毒症を呈した.食事療法と安静によって妊娠36週に経腟分娩で男児を出産した.しかし,出産後も体重増加と高血圧が持続し,産後4ヵ月には尿路結石にも罹患した.血中ACTH高値とコルチゾール高値を認めたためクッシング症候群を疑い,入院となった.精査によりクッシング病と診断し,外来で経過観察を続けた.クッシング徴候が臨床的に増悪したため,経蝶形骨洞的下垂体腫瘍摘出術を施行した.摘出した下垂体腫瘍は免疫組織学的にACTH産生性腺腫であった.現在は長時間作用ドパミンアゴニストであるカベルゴリンによる後療法を行い経過観察中であるが再発は認めていない

Languages using in journals concerning judgment/diagnosis doing by Yogo teacher and its objects

( 12 ) 15 - 26 2004

Issues and Challenges in Yogo Diagnosis for Yogo Teacher : Consideration from Nuesing Diagnosis

Ksai Atsuko, Okada Kanako, Mimura Yukari, Tokuyama Michiko

Bulletin of the Faculty of Education, Hirosaki University 92 167 - 171 2004

養護教諭の行う養護診断の確立に向けて医学領域における「診断」から考える

三村由香里, 岡田加奈子, 葛西敦子, 徳山美智子

日本保健医療行動科学学会年報 19 217 - 223 2004

蝶形骨洞内に発生した異所性ACTH産生下垂体腺腫の１例

鈴木二郎, 大塚文男, 武田昌也, 三好智子, 稲垣謙一, 岸田雅之, 三村由香里, 小倉俊郎, 橋本浩三, 槇野博史

ホルモンと臨床 52 6 - 10 2004

A lethal complication, acute necrotizing pancreatitis, of Turner's syndrome with primary hyperparathyroidism

K Masayuki, O Fumio, M Yukari, O Toshio, M Hirofumi

ENDOCRINE JOURNAL 50 ( 6 ) 835 - 836 2003.12

Hypothyroidism associated with anti-human chorionic gonadotropin antibodies secondarily produced by gonadotropin in a case of idiopathic hypothalamic hypogonadism

T Ogura, Y Mimura, F Otsuka, M Kishida, K Yokota, J Suzuki, A Nagai, S Hirakawa, H Makino, K Tobe

JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION 26 ( 11 ) 1128 - 1135 2003.11

Language：English Publisher：EDITRICE KURTIS S R L

We report a 22-yr-old male patient with idiopathic hypothalamic hypogonadism who showed secondary resistance to gonadotropin (Gn) therapy over 3 yr after successful treatment with hCG combined with human menopausal Gn. The patient simultaneously developed subclinical hypothyroidism. Endocrine examination revealed low levels of testosterone (0.3 ng/ml), free T-4 (0.91 ng/dl), and increased levels of TSH (31.1 muU/ml) in the serum. Serum autoantibodies to thyroid gland were all negative. Interestingly, thyroid function was improved after discontinuation of Gn therapy. In vitro assays by immunoprecipitation using (125)l-hCG or (125)l-TSH elucidated the presence of anti-hCG antibody in the serum 13 months after commencement of Gn therapy but anti-TSH antibody was not detected in the serum. Furthermore, the anti-hCG antibody specifically bound to hCG but not to other glycoproteins including TSH and FSH based on a competitive displacement assay. Bioassays using porcine thyroid cells revealed that the serum gamma-globulin fraction enables the suppression of cyclic AMP (cAMP) synthesis stimulated by TSH. Our findings suggest that anti-hCG and/or anti-idiotypic hCG antibodies induced by hCG therapy impaired TSH-dependent cAMP production through interfering with binding of TSH to its receptor, and this resulted in subclinical hypothyroidism in this patient. (C) 2003, Editrice Kurtis.

Role of bradykinin in renoprotective effects by angiotensin II type 1 receptor antagonist in salt-sensitive hypertension

K Yokota, M Kishida, T Ogura, J Suzuki, F Otsuka, Y Mimura, M Takeda, Y Nakamura, H Makino

HYPERTENSION RESEARCH 26 ( 3 ) 265 - 272 2003.3

Language：English Publisher：JAPANESE SOC HYPERTENSION CENT ACADEMIC SOC, PUBL OFFICE

To elucidate whether bradykinin is involved in the renoprotective effect produced by angiotensin II type 1 receptor antagonist (AT1A) in chronic salt-sensitive hypertension, Dahl salt-sensitive rats receiving a high-salt (8%) diet were treated either with an AT1A (candesartan, 1 mg/kg/day), a bradykinin B2 receptor antagonist (BKB2A; FR172357, 30 mg/kg/day) or a combination of AT1A and BKB2A for 7 weeks. None of the treatments changed the markedly increased systolic blood pressure induced by a high-salt diet. However, chronic treatment with AT1A significantly improved the histological hallmarks of renal damage-i.e., glomerular sclerosis and cell proliferation-despite the presence of severe hypertension. This beneficial action of AT1A was abolished by the concomitant administration of BKB2A. In agreement with these histologically based findings, increases in levels of creatinine clearance induced by AT1A were also reversed back to the basal levels when BKB2A was administered in conjunction with AT1A. Furthermore, urinary excretions of nitrate plus nitrite and prostaglandin E-2 increased moderately in response to the administration of AT1A alone, but not in combination with BKB2A. Thus, the blockade of bradykinin signaling abrogates the renoprotective actions of the angiotensin II type 1 (AT1) receptor antagonism. Collectively, these data show that when AT1 action is chronically blocked, endogenous bradykinin plays a pivotal role in preventing the progression of glomerular injury in salt-sensitive hypertension.

DOI： 10.1291/hypres.26.265

Relationship between adrenomedullin and vasopressin-aquaporin system under general anesthesia

T Ogura, K Morita, M Takeuchi, F Otsuka, T Iwasaki, K Oe, K Matsuura, K Tobe, Y Mimura, M Kishida, H Makino, M Hirakawa

HORMONE RESEARCH 59 ( 1 ) 30 - 34 2003

Language：English Publisher：KARGER

Aim:The roles of adrenomedullin (AM) in body fluid balance under general anesthesia were investigated. Methods: Time course changes in plasma osmolality, AM, arginine vasopressin (AVP), and urinary aquaporin 2 (AQP2) in 17 patients undergoing abdominal surgery under general anesthesia were examined. Results: Increases in plasma AM levels were observed in parallel with increases in the levels of urinary AQP2/creatinine (Cr) before induction and 90 and 180 min after initiation of anesthesia. Significant correlations between plasma AM and urinary AQP2/Cr (r = 0.62, p < 0.0001) as well as urinary AVP/Cr and AQP2/Cr (r = 0.60, p < 0.0001) were uncovered. Multivariate stepwise analysis identified plasma AM as the critical independent factor affecting urinary AQP2/Cr level. Conclusion: A novel correlation of AM and AQP2 which overlays an AVP-AQP2 system may play a key role in fluid homeostasis during general anesthesia. Copyright (C) 2003 S. Karger AG, Basel.

DOI： 10.1159/000067933

岡山大学における養護教諭養成カリキュラムの開発

高橋香代, 伊藤武彦, 三村由香里, 河内信子, 小出彌生, 門田新一郎

教科教育学研究 21 145 - 158 2003

学校保健の現場における看護的支援を要する児童・生徒の実態とこれからの支援のあり方

岡田加奈子, 三村由香里

リハビリテーション看護研究 2003

Issue and challenge in Yogo diagnosis.

Atsuko K, Okada K, Mimura Y, Tokuyama M

Japanease Journal of School Health 2003

Learning Needs with Respect to Curriculum of Nursing in Yogo teacher Training at Schools of Education

FUKUDA Hiromi, AMANO Atsuko, OKADA Kanako, HONDA Yuko, MATSUSHIMA Noriko, SHIBAKI Misako, TSUMURA Naoko, KOIDE Yayoi, MIMURA Yukari, NAKAMURA Tomoko, NISHIZAWA Yoshiko

Japanese journal of school health 45 ( 4 ) 331 - 342 2003

Learning Needs with Respect to Curriculum of Nursing in Yogo teacher Training at Schools of Education

FUKUDA Hiromi, AMANO Atsuko, OKADA Kanako, HONDA Yuko, MATSUSHIMA Noriko, SHIBAKI Misako, TSUMURA Naoko, KOIDE Yayoi, MIMURA Yukari, NAKAMURA Tomoko, NISHIZAWA Yoshiko

Japanese journal of school health 45 ( 4 ) 331 - 342 2003

Relationship between reduced serum IGF-I levels and accumulation of visceral fat in Japanese men

M Kunitomi, J Wada, K Takahashi, Y Tsuchiyama, Y Mimura, K Hida, N Miyatake, M Fujii, S Kira, K Shikata, H Makino

INTERNATIONAL JOURNAL OF OBESITY 26 ( 3 ) 361 - 369 2002.3

Language：English Publisher：NATURE PUBLISHING GROUP

OBJECTIVE: To investigate whether the changes in IGF-1 concentrations after weight reduction in Japanese overweight men are associated with changes in visceral and subcutaneous fat.
DESIGN: Cross-sectional and longitudinal clinical intervention study with exercise education.
SUBJECTS: One-hundred and twelve Japanese overweight men aged 30-59y (body mass index (BMI) 28.4+/-2.5 kg/m(2)) and 33 normal-weight men aged 30-39y (BMI 22.1+/-1.5 kg/m(2)) at baseline. From the participants, 56 randomly selected overweight men (BMI 28.8+/-2.8) were further enrolled into a 1 y exercise program.
MEASUREMENTS: Fat distribution was evaluated by visceral fat M and subcutaneous fat (S) areas measured with computed tomography scanning at umbilical levels, metabolic parameters and hormones including insulin, leptin and IGF-1 at baseline and after 1 y.
RESULTS: In 112 overweight subjects at baseline, insulin (10.5+/-5.0 muUl/ml) and leptin (6.4+/-3.7 ng/ml) significantly correlated with both V (r=0.260, P=0.0073; r=0.410, P<0.0001) and S areas (r=0.377, P<0.0001; r=0.613, P<0.0001), respectively. IGF-1 (156.8+/-48.7 muU/ml) significantly and negatively correlated with V area (r= -0.242, P=0.0125)and age (r= - 0.192, P=0.0480). In normal-weight men aged 30-39y (n=33) and age-matched subjects (n=30) selected from the 112 overweight men, the serum IGF-1 further tightly correlated with V area (r= - 0.467, P<0.0001). Visceral fat area and age were independently related to serum IGF-1 levels by multiple regression analysis. By intervention with exercise education, 56 overweight subjects showed an increase in daily steps (6224+/-2781 to 7898+/-4141 steps/day) and reduction of BMI (28.8+/-2.8 to 27.7+/-2.9). DeltaIGF-1 significantly correlated with DeltaV area (r= - 0.432, P = 0.0009) but not with DeltaS area or DeltaBMI.
CONCLUSION: The present study indicated a negative correlation between IGF-1 levels and visceral fat at baseline as well as an association between the reduction in visceral fat and increase in IGF-1 levels after an exercise intervention.

DOI： 10.1038/sj/ijo/0801899

Basedow病で発症し中枢性甲状腺機能低下に移行した1型糖尿病の一例

永瀬亮, 臼井仁美, 松田充浩, 大石徹也, 四方賢一, 槇野博史, 小倉俊郎, 三村由香里, 和田淳

糖尿病 45 ( 2 ) 162 - 162 2002.2

Japanese family with glucocorticoid-remediable aldosteronism diagnosed by long-polymerase chain reaction

K Yokota, T Ogura, M Kishida, J Suzuki, F Otsuka, Y Mimura, T Oishi, M Hirata, K Tobe, H Makino

HYPERTENSION RESEARCH 24 ( 5 ) 589 - 594 2001.9

Language：English Publisher：JAPANESE SOC HYPERTENSION CENT ACADEMIC SOC, PUBL OFFICE

We report a Japanese family with glucocorticoid-remediable aldosteronism (GRA) in whom gene abnormality was identified by the long-polymerase chain reaction (PCR) method. The proband was a 21-year-old female incidentally found to have high blood pressure (173/107 mmHg). Laboratory tests showed hypokalemia (3.7 mmol/l), and high plasma aldosterone concentration (PAC, 234 pg/ml) with suppressed plasma renin activity (PRA, < 0.1 ng/ml/h). The circadian rhythm pattern and the results of a rapid adrenocorticotrophic hormone (ACTH) test indicated ACTH-dependent changes in PAC. Imaging studies showed no adrenal mass on either side. A dexamethasone (Dexa) suppression test (1.0 mg/day orally for 7 days) showed a marked decrease of PAC 2 days after administration, and this decreased level was maintained throughout Dexa administration. High blood pressure and hypokalemia also improved during Dexa treatment. The proband's younger sister was 19 years old and had hypertension, PAC of 231 pg/ml, and PRA < 0.1 ng/ml/h. The mother was 53 years old and had hypertension, PAC of 98.5 pg/ml, and PRA < 0.1 ng/ml/h. The proband's elder sister was a 22-year-old normotensive with PAC of 110 pg/ml and PRA of 0.1 ng/ml. Long-PCR was performed for detection of the chimeric gene associated with GRA, using DNA samples from all four cases and two normal control subjects. Although the aldosterone synthase gene was expressed among all DNA samples, the chimeric gene was detected only in the proband, her younger sister and her mother. Our clinical data and genetic investigation confirmed the presence of GRA in this Japanese family.

DOI： 10.1291/hypres.24.589

Chronic treatment with amlodipine modulates adrenocortical angiotensin II receptors in spontaneously hypertensive rats

M Kishida, F Otsuka, T Ogura, H Kataoka, Y Nakamura, T Yamauchi, M Takahashi, K Yokota, Y Mimura, H Makino

LIFE SCIENCES 69 ( 1 ) 113 - 122 2001.5

Language：English Publisher：PERGAMON-ELSEVIER SCIENCE LTD

We investigated the effects of long-term treatment with calcium-antagonist, amlodipine, on angiotensin II receptors in the adrenal cortex of spontaneously hypertensive rats (SHR). Seven-week-old male SHR were treated with oral amlodipine (10 mg/kg/day) or vehicle (saline) for four weeks. Age-matched normotensive Wistar-Kyoto (WKY) rats were treated with the vehicle similar to control SHR. Systolic blood pressure (SBP) showed time-dependent increase in SHR but not in WKY rats, while amlodipine treatment significantly reduced the high SEP in SHR, Plasma renin activity was serially increased in SHR, which was further enhanced by amlodipine treatment, But the plasma aldosterone level which was increased in SHR was not changed by amlodipine. Competitive reverse transcriptase-polymerase chain reaction showed that the level of adrenocortical angiotensin II type 1 receptor (AT1R) mRNA progressively decreased in vehicle-treated SHR compared to WKY rats and that 4-week course of amlodipine treatment significantly increased AT1R mRNA in SHR to levels comparable to those in WKY rats. Amlodipine treatment reduced the level of adrenocortical angiotensin II type 2 receptor (AT2R) mRNA in SHR from 8 weeks of age. Thus, chronic amlodipine treatment differently modulates both adrenocortical AT1R and AT2R in SHR in a possibly direct manner, (C) 2001 Elsevier Science Inc. All rights reserved.

DOI： 10.1016/S0024-3205(01)01071-2

Coexistence of Graves' disease and struma ovarii: Case report and literature review

Y Mimura, M Kishida, H Masuyama, N Suwaki, J Kodama, F Otsuka, H Kataoka, T Yamauchi, T Ogura, T Kudo, H Makino

ENDOCRINE JOURNAL 48 ( 2 ) 255 - 260 2001.4

Language：English Publishing type：Book review, literature introduction, etc. Publisher：JAPAN ENDOCRINE SOCIETY

We report a rare case of Graves' disease associated with struma ovarii. A 26-year-old Japanese woman had preexisting Graves' disease and was positive for TSH receptor antibody. She had been on antithyroid medication at presentation. She noted a mass in the lower left abdomen, which was diagnosed as a left struma ovarii by radiological work-up including computed tomography, magnetic resonance imaging and scintigraphy. The surgically excised teratomatous tumor, containing cystic spaces with thyroid tissue, was histologically proved to be struma ovarii. Since thyroid function tests and TSH receptor antibody did not change after surgery, her hyperthyroidism was considered to be due to Graves' disease. Our case was diagnosed as struma ovarii before surgery using various imaging studies.

DOI： 10.1507/endocrj.48.255

The role of nitric oxide and the renin-angiotensin system in salt-restricted Dahl rats

H Kataoka, F Otsuka, T Ogura, T Yamauchi, M Kishida, M Takahashi, Y Mimura, H Makino

AMERICAN JOURNAL OF HYPERTENSION 14 ( 3 ) 276 - 285 2001.3

Language：English Publisher：ELSEVIER SCIENCE INC

To elucidate the role of nitric oxide (NO) and renin angiotensin system (RAS) in the development of salt-sensitive hypertension, we investigated the presser responses and renal histologic changes after long-term inhibition of endogenous NO synthesis in Dahl-Iwai salt-sensitive (DS) and salt-resistant (DR) rats under salt-restricted conditions that exaggerate RAS activation. Male DS and DR rats (6 weeks old) were fed with a low-salt (0.3%) diet for 5 weeks, N-G-nitro-L-arginine (L-NA: dissolved in 60 mg/L deionized water), an arginine analogue acting as a NO-inhibitor, was also administered for 5 weeks. L-NA administration induced a gradual increase in systolic blood pressure (SBP) in both strains, and the presser response in DS rats was apparently more enhanced relative to that in DR rats. Urinary nitrate plus nitrite (u-NOx) excretion was decreased by L-NA, with a significant negative correlation between SEP and u-NOx excretion in DS rats but not in DR rats. Plasma renin activity and urinary aldosterone level were significantly increased in L-NA-treated DS rats on week 5. Marked histologic changes with glomerular sclerosis and increased protein-uria and urinary N-acetyl-beta -glucosaminidase excretion were found in L-NA-treated DS rats but not DR rats. Competitive RT-PCR of mRNA extracted from the glomeruli revealed that angiotensin II type 1 receptor (AT(1)R) mRNA level was significantly lower in DS rats than in DR rats at week 2, and that L-NA administration significantly reduced glomerular AT(1)R level of DS rats at week 5, possibly because of downregulation. Our results showed that, even under sodium restriction, the presser response and renal injury induced by chronic NO inhibition were markedly more enhanced in DS rats than in DR rats, which indicates that depletion of NO participates in both the development of hypertension and glomerular injury in DS rats through a potential activation of RAS irrespective of sodium loading. These data suggest that endogenous NO is an essential determinant of salt-sensitive hypertension in DS rats. (C) 2001 American Journal of Hypertension, Ltd.

DOI： 10.1016/S0895-7061(00)01255-3

当大学における10年間の甲状腺疾患ﾑ昨年経験した甲状腺癌３例を中心にー

小倉俊郎, 松浦一陽, 塚本千秋, 絹見佳子, 内藤恵子, 福森明美, 今井あゆみ, 岸田雅之, 戸部和夫, 三村由香里

CAMPUS HEALTH 2001

Hyperthyroidism in a patient with TSH-producing pituitary adenoma coexisting with thyroid papillary adenocarcinoma

M Kishida, F Otsuka, H Kataoka, K Yokota, T Oishi, T Yamauchi, H Doihara, T Tamiya, Y Mimura, T Ogura, H Makino

ENDOCRINE JOURNAL 47 ( 6 ) 731 - 738 2000.12

Language：English Publisher：JAPAN ENDOCRINE SOCIETY

A 27-year-old woman who presented with a left thyroid nodule was found to have hyperthyroidism caused by a syndrome of inappropriate secretion of TSH. The levels of free T-3, free T-4 and TSH were 9.50 pg/mL, 4.05 ng/dL and 2.16 muU/mL, respectively. Magnetic resonance imaging of the head revealed a pituitary macroadenoma. The TSH response to TRH stimulation was normal and responses of other anterior pituitary hormones to stimulation tests were also normally preserved. Administration of octreotide with iodine successfully reversed hyperthyroidism prior to total resection of pituitary adenoma, which was followed by hemithyroidectomy of the left thyroid five months later. Histologically, the resected pituitary adenoma was a TSH-producing adenoma (TSH-oma) and the thyroid nodule was a papillary adenocarcinoma. Serum TSH diminished to undetectable levels immediately following pituitary adenomectomy but gradually normalized over nine months. Coexistence of a TSH-oma with thyroid cancer is very rare and only two similar cases have previously been documented. This combination raises the possibility that TSH may be involved in tumorigenesis in the thyroid gland.

DOI： 10.1507/endocrj.47.731

Differential effect of chronic inhibition of calcium channel and angiotensin II type 1-receptor on aldosterone synthesis in spontaneously hypertensive rats

F Otsuka, T Ogura, H Kataoka, M Kishida, M Takahashi, Y Mimura, T Yamauchi, H Makino

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 74 ( 3 ) 125 - 136 2000.10

Language：English Publisher：PERGAMON-ELSEVIER SCIENCE LTD

We have investigated the in vivo effect of chronic blockade of Ca2+-channels and angiotensin II type I (AT(1))-receptors on aldosterone (Aldo)-synthesis in the adrenal glands of spontaneously hypertensive rats (SHR). Male SHR were administered Ca2+-antagonist, amlodipine (10 mg/kg per day) or AT(1)-receptor-antagonist, TCV-116 (1 mg/kg per day) from 7 until 11 weeks of age. Systolic blood pressure (SBP) and heart rate (HR) were significantly higher in SHR than Wistar-Kyoto (WKY) rats. Both treatments resulted in equivalent and significant reduction in SEP in SHR. Aldo-secretion in SHR, which was significantly higher than in WKY rats, was profoundly suppressed by TCV-116 compared with amlodipine. Both treatments resulted in thickening of the tuna glomerulosa, which immunohistochemically contains Aldo, at the end of therapy. Competitive reverse transcription-polymerase chain reaction (RT-PCR) showed that CYP11A (P450scc) mRNA regulating the first step of Aldo-synthesis was significantly reduced from week 9 of age by amlodipine, and that CYP11A (P450aldo) mRNA regulating the last step of Aldo-synthesis was potently suppressed from 9 weeks of age by TCV-116. Our results indicate that chronic treatment with different antihypertensive agents directly modulates adrenocortical aldosterone synthesis in SHR in vivo via different mechanisms. (C) 2000 Elsevier Science Ltd. All rights reserved.

DOI： 10.1016/S0960-0760(00)00096-0

Testosterone modulates serum leptin concentrations in a male patient with hypothalamic hypogonadism

T Ogura, K Tobe, Y Mimura, F Otsuka, T Yamauchi, A Imai, C Tsukamoto, Y Iwasaki, K Matsuura

JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION 23 ( 4 ) 246 - 250 2000.4

Language：English Publisher：EDITRICE KURTIS S R L

Serial measurements of body mass index (BMI), serum concentrations of testosterone (T), estradiol (E) and leptin (L) were performed before and after gonadotropin (Gn) therapy in an 18-year-old male subject (BMI 25.4 kg/m(2)) with idiopathic hypothalamic hypogonadism (IHH). We also measured the BMI and serum concentrations of L in 99 age-matched healthy subjects. Serum L correlated significantly with BMI in control subjects (r=0.84, p<0.0001). Baseline serum concentrations of L in our case were markedly high and both T and E were very low, but On therapy resulted in a gradual decrease in L and improvement in T and E, finally reaching the control levels of BMI-matched subjects. Our results demonstrate that T is a powerful negative modulator of serum L independent of BMI in conditions associated with low T levels, such as IHH. (C) 2000, Editrice Kurtis.

Pseudomalabsorption of levothyroxine: A case report

D Ogawa, F Otsuka, Y Mimura, A Ueno, H Hashimoto, M Kishida, T Ogura, H Makino

ENDOCRINE JOURNAL 47 ( 1 ) 45 - 50 2000.2

Language：English Publisher：JAPAN ENDOCRINE SOCIETY

A 51-year-old woman who had been treated with levothyroxine sodium because of hypothyroidism after total thyroidectomy for thyroidal cancer was admitted to our hospital for persistent hypothyroidism despite large dose administration of levothyroxine (600 mu g/day). The patient complained of severe general fatigue and body weight gain. Free thyroxine, free triiodothyronine and thyrotropin levels were 0.97 ng/dl, 1.55 pg/ml and 24.51 mu U/ml, respectively, under oral administration of levothyroxine. Levothyroxine loading test performed by liquid form, pulverized tablets via nasogastric tube and intravenous administration revealed no evidence of malabsorption or metabolic disorder of levothyroxine, although oral intake of tablets was ineffective due to her factitiousness. We report here a possible case of "pseudomalabsorption of levothyroxine" to emphasize the clinical recognition of this disorder in patients with resistant hypothyroidism.

Effects of angiotensin-converting enzyme inhibitor and α1- adrenoceptor antagonist on hypertension induced in rats by long- term inhibition of nitric oxide production

M. Takahashi, F. Otsuka, T. Ogura, T. Yamauchi, H. Kataoka, M. Kishida, Y. Mimura, H. Makino

Clinical and Experimental Nephrology 4 ( 2 ) 126 - 132 2000

Language：English Publisher：Springer Japan

Background: We aimed to elucidate the involvement of the renin-angiotensin system (RAS) and sympathetic nervous system in hypertension induced by the long-term inhibition of nitric oxide (NO) production. Methods: We compared the effects of 9-week treatment with an angiotensin-converting enzyme inhibitor (ACEI), cilazapril (10 mg/kg per day), to that with an α1-adrenoceptor antagonist, doxazosin (10 mg/kg per day), on systemic blood pressure and renal histological changes in Sprague-Dawley rats continuously treated with oral N(G)nitro-L-arginine (L-NA). Results: L-NA induced renal damage associated with a significant fall in urinary nitrate and nitrite (NOx) excretion and a significant rise in systolic blood pressure. Although cilazapril and doxazosin restored urinary NOx to a similar level, only cilazapril treatment significantly suppressed the hypertensive effect of L-NA. Urinary protein excretion in L-NA-treated rats was also significantly reduced by cilazapril treatment. Histologically, treatment with cilazapril, but not doxazosin, significantly inhibited the glomerular injury of mesangial expansion and glomerular sclerosis induced by L-NA treatment. Furthermore, cilazapril significantly reduced urinary aldosterone level. Conclusion: Our findings indicate that the hypertension and hypertensive glomerular injury induced by long-term L-NA treatment were abrogated by an ACEI but not by an α1- adrenoceptor antagonist, and that the fall in high blood pressure induced by treatment with the ACEI was independent of urinary NOx excretion in this model.

DOI： 10.1007/PL00012163

Serum leptin correlates with serum uric acid but not serum testosterone in non-obese male adolescents

T Ogura, K Matsuura, F Otsuka, A Imai, C Tsukamoto, Y Mimura, Y Iwasaki, K Tobe

RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY 107 ( 1-2 ) 55 - 64 2000

Language：English Publisher：P J D PUBLICATIONS LTD

To identify the serum factors that affect circulating leptin levels, we measured the serum concentrations of leptin, testosterone (T), estradiol (E), serum alanine aminotransferase, total cholesterol and uric acid (UA) in healthy male adolescents (age, 18.3 +/- 0.1 years, n=96). We also measured body mass index (BMI), percent body fat and thickness of skin fold to assess the effect of body constitution on serum leptin level. Since serum concentration of leptin significantly correlated with BMI (r=0.820, p<0.001), we analyzed the relation-ship between leptin/BMI ratio (L/BMI) and serum parameters. Analysis of data of subjects with normal serum T level showed a significant inverse correlation between L/BMI and serum T levels (n=96, r=-0.294, p<0.005), but no such correlation was present among non-obese subjects (n=70) with BMI of +/- 20% of normal (22 kg/m(2)). There was no correlation between L/BMI and serum E level. Serum UA level significantly correlated with L/BMI in both the test group (n=96, r=0.520, p<0.001) and non-obese subjects (r=0.369, p<0.005). Stepwise multiple regression analysis showed that UA independently and significantly influenced serum leptin levels in both the test and control groups. Our results demonstrate that T weakly influences serum leptin concentration, and that UA concentrations strongly influences serum leptin in healthy male adolescents independent of their obesity level.

教育学部養護教諭養成の看護学系授業・臨床実習に対する卒業生のニーズ－フォーカス・グループ法による養護教諭対象調査より－

松島紀子, 岡田加奈子, 本田優子, 天野敦子, 福田博美, 津村直子, 芝木美沙子, 小出弥生, 三村由香里, 中村朋子, 西沢義子

日本養護教諭教育学会雑誌 3 ( 1 ) 87 - 95 2000

Treatment with a Ca2+ channel blocker, barnidipine, reduces platelet-derived growth factor B-chain mRNA in glomeruli of spontaneously hypertensive rats

M Hashimoto, T Yamauchi, T Ogura, T Oishi, Y Mimura, F Otsuka, N Kashihara, H Makino

AMERICAN JOURNAL OF NEPHROLOGY 19 ( 5 ) 615 - 621 1999.9

Language：English Publisher：KARGER

We investigated the effect of barnidipine hydrochloride, a Ca2+ chan nel blocker, on the glomerular level of mRNA expression of platelet-derived growth factor (PDGF) B-chain and transforming growth factor (TGF)-beta(1) in spontaneously hypertensive rats (SHR) with reverse transcription and polymerase chain reaction. Thirteen-week-old SHR were provided with food containing barnidipine (0.6 mg/g of food, average dose during treatment: 53 mg/kg of body mass/day) for 3 weeks. A stable reduction in systolic blood pressure relative to that of age-matched control SHR was recorded after week 1 of therapy. Although no renal histological changes were observed after 3 weeks of treatment with barnidipine, the level of expression of PDGF B-chain mRNA in glomeruli was significantly reduced relative to that in control SHR, The glomerular level of TGF-beta(1) mRNA expression was not affected by the treatment, Treatment with barnidipine significantly reduced the excretion of urinary protein, Thus, the stable reduction in systemic blood pressure by barnidipine is associated with a reduction in PDGF B-chain mRNA expression in the glomerulus and reduction in urinary protein excretion in SHR. Copyright (C) 1999 S. Karger AG, Basel.

Giant insulinoma in a patient with multiple endocrine neoplasia-type I: A case report

H Kataoka, F Otsuka, T Yamauchi, M Kishida, M Takahashi, T Tamiya, Y Mimura, T Ogura, H Makino

ENDOCRINE JOURNAL 46 ( 3 ) 429 - 435 1999.6

Language：English Publisher：JAPAN ENDOCRINE SOCIETY

We report a case of giant cystic insulinoma constituting part of multiple endocrine neoplasia (MEN) type I. A 29-year-old Japanese man presented with a history of recurrent hypoglycemic attacks. Endocrine examination showed hyperinsulinemia discordant with hypoglycemia, and a giant cystic insulinoma (11 x 10 cm) located in the pancreatic tail was detected radiologically. Hyperprolactinemia due to pituitary adenoma and hyperparathyroidism due to parathyroid hyperplasia were also present. The insulinoma, prolactinoma and hyperplastic parathyroid gland were surgically removed. Fluorescent microsatellite analysis detected loss of heterozygosity (LOH) in chromosome 11q13 in DNA samples from all resected tissues but not from white blood cells. This is a rare case of MEN type I because of the giant cystic insulinoma and the evidence of common LOH detected in all MEN type I tissues.

DOI： 10.1507/endocrj.46.429

Long-term administration of adrenocorticotropin modulates the expression of IGF-I and TGF-beta(1) mRNAs in the rat adrenal cortex

F Otsuka, T Ogura, T Yamauchi, H Kataoka, M Kishida, N Miyatake, Y Mimura, J Kageyama, H Makino

GROWTH HORMONE & IGF RESEARCH 9 ( 1 ) 41 - 51 1999.2

Language：English Publisher：CHURCHILL LIVINGSTONE

The effects of long-term adrenocorticotropin (ACTH) therapy on the expression of IGF-I and TGF-beta(1) on rat adrenal cortex was investigated. ACTH (0.1 mg/kg/day) or saline as control was injected intraperitoneally in 5-week-old Wistar rats every day for 4 weeks. ACTH significantly increased adrenal weight (P<0.05) and serum corticosterone (P<0.05). Competitive RT-PCR analysis on the adrenocortical mRNA showed increased IGF-I (P<0.01) at 4 weeks of ACTH and increased TGF-beta(1) (P<0.01) at 1 week of ACTH compared the control group. ACTH also significantly increased proliferating cell nuclear antigen mRNA level (P<0.01), at 4 weeks of treatment, which correlated with IGF-I level (P<0.01), but correlated negatively with ACTH-stimulated TGF-beta(1) level (P<0.05). There was a weak correlation between IGF-I and serum corticosterone (P<0.05), and between TGF-beta(1) mRNA levels and serum corticosterone concentration (P<0.05). Histologically, ACTH induced hypertrophy in the zona fasciculata cells and increased the clear cells containing lipid deposits. Immunohistochemistry showed that IGF-I peptide was mainly expressed in the periphery of the zona fasciculata at 4 weeks of ACTH therapy, while the same therapy caused a slight increase in TGF-beta(1) expression in the same area. Our results show that an increase in adrenocortical growth resulting from ACTH treatment is associated with an increase in IGF-I mRNA expression but only a transient increase in TGF-beta(1) mRNA expression. (C) 1999 Churchill Livingstone.

DOI： 10.1054/ghir.1998.0086

Present status and problems of visiting nurse station

MIMURA Yukari, MAKINO Hirofumi, OKA Youko, WATANABE Kimiko, KANAMI Hifumi, MORIWAKI Kazuhiko, KATAYAMA Eriko, FUZIWARA Hiromichi, SADAKANE Takazi, SATOU Yoshiyuki, HAYASHI Ippei, SATOU Kamehiro, OGURA Toshio

111 ( 3 ) 85 - 93 1999

Effect of treatment with barnidipine, a calcium channel blocker, on platelet-derived growth factor B chain from glomerulus in spontaneously hypertensive rats.

Hashimoto M, Yamauchi T, Ogura T, Oishi T, Mimura Y, Otsuka F, Kashihara N, Makino H

Am J Nephrol 1999

Renal AT(1) receptor: Autoradiographic localization and quantification in rat

N Asano, T Ogura, Y Mimura, M Kishida, H Kataoka, F Otsuka, T Yamauchi, H Makino

RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY 100 ( 2 ) 161 - 170 1998.5

Language：English Publisher：P J D PUBLICATIONS LTD

To elucidate the precise localization of angiotensin II (Ang II) type 1 (AT(1)) receptor in the kidney, we utilized in vitro macro- and micro-autoradiography (ARG) of [H-3]-Ang II bindings to the Wistar rat kidney in the presence of L158,809, a specific non-peptide AT(1) receptor antagonist. Besides, we estimated the density of renal AT, receptors using the quantification of macro-ARG. The density of [H-3]-Ang II binding to renal tissue was concentration-dependent in both renal cortex and medulla Although the addition with 500 nM arginine vasopressin and 500 nM atrial natriuretic peptide had no effect on [H-3]-Ang II, the total binding of [H-3] Ang II completely displaced by the addition with 500 nM unlabeled Ang II or L-158,809. Macro-ARG revealed that the amount of both Ang II and AT, receptors in the renal medulla gready exceeded those in the renal cortex In the medulla, the density of these receptors was not localized on the outer medulla but was confirmed mainly to the inner medulla, especially to the inner zone and longitudinal bands. Since the density and localization of AT(1) receptors was consistent with that of total Ang II receptors, it appears that AT(1) receptors comprise most of the Ang II receptors in the kidney. Micro-ARG revealed that Ang II receptors were mainly located in the glomerulus and proximal tubules of the renal cortex, as well as on the circumferences of vessels and the vasa recta of the renal medulla. The present study established a method for ARO of AT(1) receptors in the kidney as well as a method for quantifying the macro-ARG.

Renal AT(1) receptor: Computerized quantification in spontaneously hypertensive rats and DOCA-salt rats

N Asano, T Ogura, Y Mimura, F Otsuka, M Kishida, M Hashimoto, T Yamauchi, H Makino

RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY 100 ( 2 ) 171 - 180 1998.5

Language：English Publisher：P J D PUBLICATIONS LTD

To assess the involvement of angiotensin II (Ang II) in the regulation of blood pressure, we investigated the alterations of renal Ang II type 1 (AT(1)) receptors in two different models of hypertension; i.e., in spontaneously hypertensive rats (SHR) and deoxycorticosterone acetate (DOCA)-salt hypertensive rats by using the method for quantification of in vitro macroautoradiography (ARG). In the SHR model, although the number of cortical AT(1) receptors equaled that of Wistar Kyoto (WKY) rats at 4 and 12 weeks of age, the number of medullary AT(1) receptors in the 4-week-old SHR animals was significantly lower than that in age-matched WKY rats, and increased by 12 weeks of age. The renal AT(1) receptor number in DOCA-salt hypertensive rats was significantly higher than that in control rats. The amount of these receptors also increased with age in both DOCA-salt hypertensive rats and control rats. These findings indicate that the development of medullary AT(1) receptors in early stages of hypertension in the SHR model differs from that in WKY rats or DOCA-salt hypertensive rats. This suggests that renal AT(1) receptors may contribute to the hypertension seen in the SHR group. The renal AT(1) receptors appear to be up-regulated in early stages of DOCA-salt-induced hypertension as well.

In vitro Macro-and Microautoradiographic Localization of V1 and V2 Receptors in the Rat Kidney Using OPC-21268 and OPC-31260

Yukari Mimura, Toshio Ogura, Nobuhiko Hayakawa, Fumio Otsuka, Masami Hashimoto, Takayoshi Yamauchi, Hirofumi Makino, Norio Ogawa

Nephron 76 ( 3 ) 331 - 336 1997

Language：English

To elucidate the precise localization of vasopressin(VP) Vj and V2 receptors in the kidney, we utilized in vitro macroautoradiography (macro-ARG) and microautoradiography (micro-ARG) of these receptors in Wistar rat kidneys.This was done by using OPC-21268 and OPC-31260, two newly developed selective Vi (OPC-21268) and V2 (OPC-31260)receptor antagonists. For macro-ARG, 10-μm kidney sections were incubated with Tris-HCl buffer containing [3H]-VP with or without unlabeled ligand (VP, OPC-21268, orOPC-31260) at 20°C for 40 min. These sections were then loaded intoX-ray cassettes with Hyperfilm-[3H] and exposed inthe dark for 2 months. The autoradiograms were quantitativelyanalyzed by using the research analysis system RAS 1,000
the V1 and V2 receptors were quantitated by inftracting the nonspecific binding (incubatedwith OPC-21268 and OPC-31260, respectively) from the total binding. To assess a more precise localization of the V1and V2 receptors, we also investigated the micro-ARG of the renal V1 and V2 receptorsby dipping the kidney section slides used for macro-ARG into a photographic emulsion and observing the receptors under light microscopy. [3H]-VP binding to the rat kidney was completely displaced by unlabeled excess VP, but not by unlabeled angiotensin II, indicating that [3H]-VP binding was specific for VP receptors. Computerized quantification showed that V2 receptors, visualized by OPC-31260, were the predominant type of VP receptor in the kidney. Conversely, V1receptors, visualized by OPC-21268, werefewer in number. V) receptors were partly localized to the glomerulus, cortical vessels, interstitial cells, and the medullary vessels. The V2receptors localized to the collecting ducts and medullary tubules. Our findings indicated that renal V1 and V2 receptors can be detected by in vitro macro- and micro-ARG by using OPC-21268 and OPC-31260. © 1997 S.Karger AG, Basel.

DOI： 10.1159/000190200

In vitro Macro-and Microautoradiographic Localization of V1 and V2 Receptors in the Rat Kidney Using OPC-21268 and OPC-31260

Yukari Mimura, Toshio Ogura, Nobuhiko Hayakawa, Fumio Otsuka, Masami Hashimoto, Takayoshi Yamauchi, Hirofumi Makino, Norio Ogawa

Nephron 76 ( 3 ) 331 - 336 1997

Language：English

To elucidate the precise localization of vasopressin(VP) Vj and V2 receptors in the kidney, we utilized in vitro macroautoradiography (macro-ARG) and microautoradiography (micro-ARG) of these receptors in Wistar rat kidneys.This was done by using OPC-21268 and OPC-31260, two newly developed selective Vi (OPC-21268) and V2 (OPC-31260)receptor antagonists. For macro-ARG, 10-μm kidney sections were incubated with Tris-HCl buffer containing [3H]-VP with or without unlabeled ligand (VP, OPC-21268, orOPC-31260) at 20°C for 40 min. These sections were then loaded intoX-ray cassettes with Hyperfilm-[3H] and exposed inthe dark for 2 months. The autoradiograms were quantitativelyanalyzed by using the research analysis system RAS 1,000
the V1 and V2 receptors were quantitated by inftracting the nonspecific binding (incubatedwith OPC-21268 and OPC-31260, respectively) from the total binding. To assess a more precise localization of the V1and V2 receptors, we also investigated the micro-ARG of the renal V1 and V2 receptorsby dipping the kidney section slides used for macro-ARG into a photographic emulsion and observing the receptors under light microscopy. [3H]-VP binding to the rat kidney was completely displaced by unlabeled excess VP, but not by unlabeled angiotensin II, indicating that [3H]-VP binding was specific for VP receptors. Computerized quantification showed that V2 receptors, visualized by OPC-31260, were the predominant type of VP receptor in the kidney. Conversely, V1receptors, visualized by OPC-21268, werefewer in number. V) receptors were partly localized to the glomerulus, cortical vessels, interstitial cells, and the medullary vessels. The V2receptors localized to the collecting ducts and medullary tubules. Our findings indicated that renal V1 and V2 receptors can be detected by in vitro macro- and micro-ARG by using OPC-21268 and OPC-31260. © 1997 S.Karger AG, Basel.

DOI： 10.1159/000190200

Alterations of renal V1 and V2 receptors in spontaneously hypertensive rats and doca-salt hypertensive rats using computerized quantification for macro-autoradiogram

Yukari Mimura, Toshio Ogura, Hideo Kataoka, Teisuya Oishi, Naoko Asano, Masayuki Kishida, Takayoshi Yamauchi, Norio Ogawa, Hirofumi Makino

Research Communications in Molecular Pathology and Pharmacology 95 ( 1 ) 43 - 56 1997

Language：English

We previously examined the precise localization of vasopressin (VP) V1 and V2 receptors in the rat kidney using in vitro macro- and micro- autoradiography (ARG), and established the methodology of quantification for macro-ARG. In this study, to elucidate the role of VP in hypertension, we investigated the change within V1 and V2 receptors in the kidney of spontaneously hypertensive rats (SHR) and deoxycorticosterone acetate (DOCA)- salt hypertensive rats at different hypertensive stages. In SHR, although medullary V1 and V2 receptors decreased compared with age-matched Wistar- Kyoto (WKY) rats at the developmental hypertensive stage, these receptors increased once hypertension was established. Conversely, in DOCA-salt hypertensive rats, both renal V1 and V2 receptors decreased with elevated blood pressure. Therefore, expression of renal V1 and V2 receptors proved to be different between two models of hypertension, SHR and DOCA-salt hypertensive rats, when their blood pressure increases. In DOCA-salt hypertensive rats, renal V1 and V2 receptors may be down-regulated secondary to the high blood VP level. In SHR, the increase in renal V1 and V2 receptors may have a role in the development of high blood pressure in this strain of rats.

A patient with sarcoidosis associated with recurrent urolithiasis and tubular injury caused by calcium deposition

Yukari Mimura, Yohei Maeshima, Toshio Ogura, Naoki Kashihara, Kenji Kawabata, Nobuhiko Hayakawa, Yoshio Nagake, Kazue Hironaka, Hirofumi Makino, Zensuke Ota, Jingo Kageyama

Japanese Journal of Nephrology 38 ( 3 ) 141 - 145 1996

Language：English

A 38-year-old woman was hospitalized in January, 1994 with renal dysfunction and hypercalcemia. Before admission, she was diagnosed as having urolithiasis, and had been treated twice with extracorporeal shock wave lithotripsy (ESWL). Ophthalmologically, she exhibited iritis and secondary glaucoma. Hypercalcemia, an extremely low titer of parathyroid hormone (PTH), and elevation of angiotensin-converting enzyme (ACE) and lysozyme activity were noted. These findings suggested sarcoidosis, although the chest X-ray showed only fibrotic changes. Hypercalcemia was suspected of having been caused secondarily by sarcoidosis. Since her laboratory data also showed renal dysfunction and abnormal unnalysis, a renal biopsy was performed. The histological findings indicated a tubular and interstitial disorder without glomerular abnormality
calcium deposition, which was detected by X-ray energy dispersive analysis, was observed in the tubular cytoplasm. Administration of prednisolone alleviated the renal dysfunction and decreased the elevation of ACE activity and lysozyme level of the blood. Sarcoidosis is sometimes associated with hypercalcemia, but rarely with renal dysfunction. These findings suggested that sarcoidosis may be associated with renal dysfunction due to tubular injury caused by calcium deposition in the tubules, and that glucocorticoid therapy was effective for these disorders.

DOI： 10.14842/jpnjnephrol1959.38.141

A patient with sarcoidosis associated with recurrent urolithiasis and tubular injury caused by calcium deposition

Yukari Mimura, Yohei Maeshima, Toshio Ogura, Naoki Kashihara, Kenji Kawabata, Nobuhiko Hayakawa, Yoshio Nagake, Kazue Hironaka, Hirofumi Makino, Zensuke Ota, Jingo Kageyama

Japanese Journal of Nephrology 38 ( 3 ) 141 - 145 1996

Language：English

A 38-year-old woman was hospitalized in January, 1994 with renal dysfunction and hypercalcemia. Before admission, she was diagnosed as having urolithiasis, and had been treated twice with extracorporeal shock wave lithotripsy (ESWL). Ophthalmologically, she exhibited iritis and secondary glaucoma. Hypercalcemia, an extremely low titer of parathyroid hormone (PTH), and elevation of angiotensin-converting enzyme (ACE) and lysozyme activity were noted. These findings suggested sarcoidosis, although the chest X-ray showed only fibrotic changes. Hypercalcemia was suspected of having been caused secondarily by sarcoidosis. Since her laboratory data also showed renal dysfunction and abnormal unnalysis, a renal biopsy was performed. The histological findings indicated a tubular and interstitial disorder without glomerular abnormality
calcium deposition, which was detected by X-ray energy dispersive analysis, was observed in the tubular cytoplasm. Administration of prednisolone alleviated the renal dysfunction and decreased the elevation of ACE activity and lysozyme level of the blood. Sarcoidosis is sometimes associated with hypercalcemia, but rarely with renal dysfunction. These findings suggested that sarcoidosis may be associated with renal dysfunction due to tubular injury caused by calcium deposition in the tubules, and that glucocorticoid therapy was effective for these disorders.

DOI： 10.14842/jpnjnephrol1959.38.141

Effect of vasopressin V1-and V2-receptor stimulation on blood pressure in DOCA-salt hypertensive rats. (共著)

Mimura Yukari, Ogura Toshio, Yamauchi Takayoshi, Otsuka Fumio, Oishi Tetsuya, Harada Kazushi, Hashimoto Masami, Ota Zensuke

Acta Med Okayama 49 ( 4 ) 187 - 194 1995.8

Effect of vasopressin V1- and V2-receptor stimulation on blood pressure in DOCA-salt hypertensive rats.

Mimura Yukari, Ogura Toshio, Yamauchi Takayoshi, Otsuka Fumio, Oishi Tetsuya, Harada Kazushi, Hashimoto Masami, Ota Zensuke

Acta Medica Okayama 49 ( 4 ) 187 - 194 1995.8

In vitro micro-autoradiography of atrial natriuretic peptide in biopsy specimens from patients with renal diseases. (jointly worked)

Ogura T, Asano N, Katayama E, Oishi T, Mimura Y, Hironaka K, Kashihara N, Makino H, Ota Z, Ogawa N

Journal of Medicine 25 203 - 217 1994

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